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Auteur : Grafiati
Publié le 11 mars 2023

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1

Soares, Hugo R., Ana I. Almeida, Hélio A. Tomás, Paula M. Alves et Ana S. Coroadinha. « Flexible pseudotyping of retrovirus using recombinase-mediated cassette exchange ». Biotechnology Letters 40, no 4 (20 janvier 2018) : 633–39. http://dx.doi.org/10.1007/s10529-018-2515-6.

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Nakano, M. « Production of viral vectors using recombinase-mediated cassette exchange ». Nucleic Acids Research 33, no 8 (28 avril 2005) : e76-e76. http://dx.doi.org/10.1093/nar/gni074.

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Premsrirut, Prem K., Lukas E. Dow, Youngkyu Park, Gregory J. Hannon et Scott W. Lowe. « Creating Transgenic shRNA Mice by Recombinase-Mediated Cassette Exchange ». Cold Spring Harbor Protocols 2013, no 9 (septembre 2013) : pdb.prot077057. http://dx.doi.org/10.1101/pdb.prot077057.

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Louwerse, Jeanine D., Miranda C. M. van Lier, Dirk M. van der Steen, Clementine M. T. de Vlaam, Paul J. J. Hooykaas et Annette C. Vergunst. « Stable Recombinase-Mediated Cassette Exchange in Arabidopsis Using Agrobacterium tumefaciens ». Plant Physiology 145, no 4 (5 octobre 2007) : 1282–93. http://dx.doi.org/10.1104/pp.107.108092.

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Murray, Johanne M., Adam T. Watson et Antony M. Carr. « Identifying Products of Recombinase-Mediated Cassette Exchange (RMCE) inSchizosaccharomyces pombe ». Cold Spring Harbor Protocols 2016, no 5 (mai 2016) : pdb.prot090944. http://dx.doi.org/10.1101/pdb.prot090944.

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Turan, Soeren, Melanie Galla, Ellen Ernst, Junhua Qiao, Christine Voelkel, Bernhard Schiedlmeier, Christoph Zehe et Juergen Bode. « Recombinase-Mediated Cassette Exchange (RMCE) : Traditional Concepts and Current Challenges ». Journal of Molecular Biology 407, no 2 (mars 2011) : 193–221. http://dx.doi.org/10.1016/j.jmb.2011.01.004.

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Nonet, Michael L. « Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange ». Genetics 215, no 4 (8 juin 2020) : 903–21. http://dx.doi.org/10.1534/genetics.120.303388.

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The application of CRISPR technology has greatly facilitated the creation of transgenic Caenorhabditis elegans lines. However, methods to insert multi-kilobase DNA constructs remain laborious even with these advances. Here, I describe a new approach for introducing large DNA constructs into the C. elegans genome at specific sites using a combination of Flp and Cre recombinases. The system utilizes specialized integrated landing sites that express GFP ubiquitously flanked by single loxP, FRT, and FRT3 sites. DNA sequences of interest are inserted into an integration vector that contains a sqt-1 self-excising cassette and FRT and FRT3 sites. Plasmid DNA is injected into the germline of landing site animals. Transgenic animals are identified as Rol progeny, and the sqt-1 marker is subsequently excised with heat shock Cre expression. Integration events were obtained at a rate of approximately one integration per three injected F0 animals—a rate substantially higher than any current approach. To demonstrate the robustness of the approach, I compared the efficiency of the Gal4/UAS, QF (and QF2)/QUAS, tetR(and rtetR)/tetO, and LexA/lexO bipartite expression systems by assessing expression levels in combinations of driver and reporter GFP constructs and a direct promoter GFP fusion each integrated at multiple sites in the genome. My data demonstrate that all four bipartite systems are functional in C. elegans. Although the new integration system has several limitations, it greatly reduces the effort required to create single-copy insertions at defined sites in the C. elegans genome.
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Sorrell, David A., Claire J. Robinson, Jo-Ann Smith et Andreas F. Kolb. « Recombinase mediated cassette exchange into genomic targets using an adenovirus vector ». Nucleic Acids Research 38, no 11 (5 avril 2010) : e123-e123. http://dx.doi.org/10.1093/nar/gkq192.

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Sun, Florence F., Justine E. Johnson, Martin P. Zeidler et Jack R. Bateman. « Simplified Insertion of Transgenes Onto Balancer Chromosomes via Recombinase-Mediated Cassette Exchange ». G3&#58 ; Genes|Genomes|Genetics 2, no 5 (mai 2012) : 551–53. http://dx.doi.org/10.1534/g3.112.002097.

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Cesari, Francesca, Verena Rennekampff, Kristina Vintersten, Lam Giang Vuong, Jost Seibler, J�rgen Bode, Franziska F. Wiebel et Alfred Nordheim. « Elk-1 knock-out mice engineered by Flp recombinase-mediated cassette exchange ». genesis 38, no 2 (2004) : 87–92. http://dx.doi.org/10.1002/gene.20003.

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Turan, Soeren, Johannes Kuehle, Axel Schambach, Christopher Baum et Juergen Bode. « Multiplexing RMCE : Versatile Extensions of the Flp-Recombinase-Mediated Cassette-Exchange Technology ». Journal of Molecular Biology 402, no 1 (septembre 2010) : 52–69. http://dx.doi.org/10.1016/j.jmb.2010.07.015.

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Kameyama, Yujiro, Yoshinori Kawabe, Akira Ito et Masamichi Kamihira. « Accumulative gene integration technique using recombinase mediate cassette exchange ». Journal of Bioscience and Bioengineering 108 (novembre 2009) : S18. http://dx.doi.org/10.1016/j.jbiosc.2009.08.488.

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Nanto, Kazuya, Kanna Sato, Yoshihiro Katayama et Hiroyasu Ebinuma. « Expression of a transgene exchanged by the recombinase-mediated cassette exchange (RMCE) method in plants ». Plant Cell Reports 28, no 5 (25 février 2009) : 777–85. http://dx.doi.org/10.1007/s00299-009-0683-5.

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Williams, Kristina, Xiaoyun Zhao et Wallace S. Chick. « A toolkit for transgenesis at the ROSA26 locus by recombinase-mediated cassette exchange ». F1000Research 2 (29 juillet 2013) : 162. http://dx.doi.org/10.12688/f1000research.2-162.v1.

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We describe a toolkit to perform transgenesis at the ROSA26 locus by recombinase-mediated cassette exchange that will eliminate the inherent problem of random insertion via traditional pronuclear injection. A recombination site-tagged embryonic stem (ES) cell line and two cloning vectors were constructed to facilitate the generation of targeted ES cells with the transgene of interest at ROSA26. The experimental procedure is simple and efficient, and can be readily adapted in many laboratories for rapid generation of transgenic mice.
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Li, Zhongsen, Aiqiu Xing, Bryan P. Moon, Richard P. McCardell, Kelly Mills et S. Carl Falco. « Site-Specific Integration of Transgenes in Soybean via Recombinase-Mediated DNA Cassette Exchange ». Plant Physiology 151, no 3 (8 mai 2009) : 1087–95. http://dx.doi.org/10.1104/pp.109.137612.

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Lee, Hong Jo, Hyung Chul Lee, Young Min Kim, Young Sun Hwang, Young Hyun Park, Tae Sub Park et Jae Yong Han. « Site‐specific recombination in the chicken genome using Flipase recombinase‐mediated cassette exchange ». FASEB Journal 30, no 2 (6 octobre 2015) : 555–63. http://dx.doi.org/10.1096/fj.15-274712.

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Watson, Adam T., Valerie Garcia, Neil Bone, Antony M. Carr et John Armstrong. « Gene tagging and gene replacement using recombinase-mediated cassette exchange in Schizosaccharomyces pombe ». Gene 407, no 1-2 (janvier 2008) : 63–74. http://dx.doi.org/10.1016/j.gene.2007.09.024.

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Noskov, Vladimir N., Li Ma, Stephen Chen et Ray-Yuan Chuang. « Recombinase-mediated cassette exchange (RMCE) system for functional genomics studies in Mycoplasma mycoides ». Biological Procedures Online 17, no 1 (2015) : 6. http://dx.doi.org/10.1186/s12575-015-0016-8.

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Potter, Leah A., Eunyoung Choi, Susan B. Hipkens, Christopher V. E. Wright et Mark A. Magnuson. « A recombinase-mediated cassette exchange-derived cyan fluorescent protein reporter allele for Pdx1 ». genesis 50, no 4 (6 janvier 2012) : 384–92. http://dx.doi.org/10.1002/dvg.20804.

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Turan, Soeren, Christoph Zehe, Johannes Kuehle, Junhua Qiao et Juergen Bode. « Recombinase-mediated cassette exchange (RMCE) — A rapidly-expanding toolbox for targeted genomic modifications ». Gene 515, no 1 (février 2013) : 1–27. http://dx.doi.org/10.1016/j.gene.2012.11.016.

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Feng, Yong-Qing, Matthew C. Lorincz, Steve Fiering, John M. Greally et Eric E. Bouhassira. « Position Effects Are Influenced by the Orientation of a Transgene with Respect to Flanking Chromatin ». Molecular and Cellular Biology 21, no 1 (1 janvier 2001) : 298–309. http://dx.doi.org/10.1128/mcb.21.1.298-309.2001.

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ABSTRACT We have inserted two expression cassettes at tagged reference chromosomal sites by using recombinase-mediated cassette exchange in mammalian cells. The three sites of integration displayed either stable or silencing position effects that were dominant over the different enhancers present in the cassettes. These position effects were strongly dependent on the orientation of the construct within the locus, with one orientation being permissive for expression and the other being nonpermissive. Orientation-specific silencing, which was observed at two of the three site tested, was associated with hypermethylation but not with changes in chromatin structure, as judged by DNase I hypersensitivity assays. Using CRE recombinase, we were able to switch in vivo the orientation of the transgenes from the permissive to the nonpermissive orientation and vice versa. Switching from the permissive to the nonpermissive orientation led to silencing, but switching from the nonpermissive to the permissive orientation did not lead to reactivation of the transgene. Instead, transgene expression occurred dynamically by transcriptional oscillations, with 10 to 20% of the cells expressing at any given time. This result suggested that the cassette had been imprinted (epigenetically tagged) while it was in the nonpermissive orientation. Methylation analysis revealed that the methylation state of the inverted cassettes resembled that of silenced cassettes except that the enhancer had selectively lost some of its methylation. Sorting of the expressing and nonexpressing cell populations provided evidence that the transcriptional oscillations of the epigenetically tagged cassette are associated with changes in the methylation status of regulatory elements in the transgene. This suggests that transgene methylation is more dynamic than was previously assumed.
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22

Lu, Jun, et Randy L. Johnson. « Insertion of large-insert clones into the mouse genome by recombinase-mediated cassette exchange ». Developmental Biology 295, no 1 (juillet 2006) : 379–80. http://dx.doi.org/10.1016/j.ydbio.2006.04.165.

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Malchin, Natalia, Tatiana Molotsky, Ezra Yagil, Alexander B. Kotlyar et Mikhail Kolot. « Molecular analysis of recombinase-mediated cassette exchange reactions catalyzed by integrase of coliphage HK022 ». Research in Microbiology 159, no 9-10 (novembre 2008) : 663–70. http://dx.doi.org/10.1016/j.resmic.2008.09.005.

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Schetelig, M. F., Y. Yan, Y. Zhao et A. M. Handler. « Genomic targeting by recombinase-mediated cassette exchange in the spotted wing drosophila, Drosophila suzukii ». Insect Molecular Biology 28, no 2 (16 octobre 2018) : 187–95. http://dx.doi.org/10.1111/imb.12537.

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Fernandes, Fabiana, Mafalda M. Dias, João Vidigal, Marcos F. Q. Sousa, Marco Patrone, Ana P. Teixeira et Paula M. Alves. « Production of rotavirus core-like particles in Sf9 cells using recombinase-mediated cassette exchange ». Journal of Biotechnology 171 (février 2014) : 34–38. http://dx.doi.org/10.1016/j.jbiotec.2013.11.020.

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Feng, Yong-Qing, Jost Seibler, Raouf Alami, Andrew Eisen, Karen A. Westerman, Philippe Leboulch, Steven Fiering et Eric E. Bouhassira. « Site-specific chromosomal integration in mammalian cells : highly efficient CRE recombinase-mediated cassette exchange ». Journal of Molecular Biology 292, no 4 (octobre 1999) : 779–85. http://dx.doi.org/10.1006/jmbi.1999.3113.

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Feng, Yong-Qing, Renaud Warin, Taihao Li, Emmanuel Olivier, Arnaud Besse, Amanda Lobell, Haiqing Fu, Chii Mei Lin, Mirit I. Aladjem et Eric E. Bouhassira. « The Human β-Globin Locus Control Region Can Silence as Well as Activate Gene Expression ». Molecular and Cellular Biology 25, no 10 (15 mai 2005) : 3864–74. http://dx.doi.org/10.1128/mcb.25.10.3864-3874.2005.

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ABSTRACT Using recombinase-mediated cassette exchange to test multiple transgenes at the same site of integration, we demonstrate a novel chromatin context-dependent silencer activity of the β-globin locus control region (LCR). This silencer activity requires DNase I hypersensitive sites HS2 and HS3 but not HS4. After silencing, the silenced cassettes adopt a typical closed chromatin conformation (histone H3 and H4 deacetylation, histone H3-K4 methylation, DNA methylation, and replication in late S phase). In the absence of the LCR at the same site of integration, the chromatin remains decondensed. We demonstrate that the LCR is necessary but not sufficient to trigger these chromatin changes. We also provide evidence that this novel silencing activity is caused by transcriptional interference triggered by activation of transcription in the flanking sequences by the LCR.
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Weng, Ruifen, Ya-Wen Chen, Natascha Bushati, Adam Cliffe et Stephen M. Cohen. « Recombinase-Mediated Cassette Exchange Provides a Versatile Platform for Gene Targeting : Knockout of miR-31b ». Genetics 183, no 1 (29 juin 2009) : 399–402. http://dx.doi.org/10.1534/genetics.109.105213.

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Minorikawa, Sachiko, et Manabu Nakayama. « Recombinase-mediated cassette exchange (RMCE) and BAC engineering via VCre/VloxP and SCre/SloxP systems ». BioTechniques 50, no 4 (avril 2011) : 235–46. http://dx.doi.org/10.2144/000113649.

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Malchin, Natalia, Tatiana Molotsky, Ilya Borovok, Yuri Voziyanov, Alexander B. Kotlyar, Ezra Yagil et Mikhail Kolot. « High Efficiency of a Sequential Recombinase-Mediated Cassette Exchange Reaction in Escherichia coli ». Journal of Molecular Microbiology and Biotechnology 19, no 3 (2010) : 117–22. http://dx.doi.org/10.1159/000321497.

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Wong, E. T. « Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange ». Nucleic Acids Research 33, no 17 (25 septembre 2005) : e147-e147. http://dx.doi.org/10.1093/nar/gni145.

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Liu, Kai, Susan Hipkens, Tao Yang, Robert Abraham, Wei Zhang, Nagesh Chopra, Bjorn Knollmann, Mark A. Magnuson et Dan M. Roden. « Recombinase-mediated cassette exchange to rapidly and efficiently generate mice with human cardiac sodium channels ». genesis 44, no 11 (novembre 2006) : 556–64. http://dx.doi.org/10.1002/dvg.20247.

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Chen, Chuan, Nan Li, Yun Zhao et Haiying Hang. « Coupling recombinase-mediated cassette exchange with somatic hypermutation for antibody affinity maturation in CHO cells ». Biotechnology and Bioengineering 113, no 1 (31 juillet 2015) : 39–51. http://dx.doi.org/10.1002/bit.25541.

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Ordovás, Laura, Ruben Boon, Mariaelena Pistoni, Yemiao Chen, Esther Wolfs, Wenting Guo, Rangarajan Sambathkumar et al. « Efficient Recombinase-Mediated Cassette Exchange in hPSCs to Study the Hepatocyte Lineage Reveals AAVS1 Locus-Mediated Transgene Inhibition ». Stem Cell Reports 5, no 5 (novembre 2015) : 918–31. http://dx.doi.org/10.1016/j.stemcr.2015.09.004.

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Ordovás, Laura, Ruben Boon, Mariaelena Pistoni, Yemiao Chen, Esther Wolfs, Wenting Guo, Rangarajan Sambathkumar et al. « Efficient Recombinase-Mediated Cassette Exchange in hPSCs to Study the Hepatocyte Lineage Reveals AAVS1 Locus-Mediated Transgene Inhibition ». Stem Cell Reports 10, no 2 (février 2018) : 673. http://dx.doi.org/10.1016/j.stemcr.2018.01.034.

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Bouhassira, Eric E., Karen Westerman et Philippe Leboulch. « Transcriptional Behavior of LCR Enhancer Elements Integrated at the Same Chromosomal Locus by Recombinase-Mediated Cassette Exchange ». Blood 90, no 9 (1 novembre 1997) : 3332–44. http://dx.doi.org/10.1182/blood.v90.9.3332.

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Abstract Efficient integration of transgenes at preselected chromosomal locations was achieved in mammalian cells by recombinase-mediated-cassette-exchange (RMCE), a novel procedure that makes use of the CRE recombinase together with Lox sites bearing different spacer regions. We have applied RMCE to the study of the human β-globin gene Locus Control Region by integrating at the same genetic locus in MEL cells, a LacZ gene driven by the human β-globin promoter linked to HS2 and HS3 alone or in combination with HS4. Expression studies at the cell population level and in individual cells before and after induction of differentiation with hemin or DMSO show that the presence of these enhancers is associated with variegated patterns of expression. We were able to show that the LCR fragments tested act by controlling both the probability of expression and the rate of transcription of the linked β-globin promoter. Both of these factors were also dependent on the state of differentiation of the MELc and on the presence of a second transcription unit located in cis. The ability to manipulate by RMCE constructs integrated into chromosomes should help in the creation of complex, rationally designed, artificial genetic loci.
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Baumann, Martina, Elisabeth Gludovacz, Sabine Vcelar et Nicole Borth. « Utilization of recombinase mediated cassette exchange (RMCE) for the generation of recombinant CHO cell lines with defined expression properties ». New Biotechnology 31 (juillet 2014) : S4. http://dx.doi.org/10.1016/j.nbt.2014.05.1623.

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Coroadinha, A. S., R. Schucht, L. Gama-Norton, D. Wirth, H. Hauser et M. J. T. Carrondo. « The use of recombinase mediated cassette exchange in retroviral vector producer cell lines : Predictability and efficiency by transgene exchange ». Journal of Biotechnology 124, no 2 (juillet 2006) : 457–68. http://dx.doi.org/10.1016/j.jbiotec.2006.01.037.

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Schetelig, Marc F., et Alfred M. Handler. « A Functional Comparison of the3xP3Promoter by Recombinase-Mediated Cassette Exchange inDrosophilaand a Tephritid Fly,Anastrepha suspensa ». G3&#58 ; Genes|Genomes|Genetics 3, no 4 (11 mars 2013) : 687–93. http://dx.doi.org/10.1534/g3.112.005488.

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Zhang, Lin, Mara C. Inniss, Shu Han, Mark Moffat, Heather Jones, Baohong Zhang, Wendy L. Cox, James R. Rance et Robert J. Young. « Recombinase-mediated cassette exchange (RMCE) for monoclonal antibody expression in the commercially relevant CHOK1SV cell line ». Biotechnology Progress 31, no 6 (13 octobre 2015) : 1645–56. http://dx.doi.org/10.1002/btpr.2175.

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Elias, Amer, Hala Kassis, Suha Abd Elkader, Natasha Gritsenko, Alessio Nahmad, Hodaya Shir, Liana Younis et al. « HK022 bacteriophage Integrase mediated RMCE as a potential tool for human gene therapy ». Nucleic Acids Research 48, no 22 (3 décembre 2020) : 12804–16. http://dx.doi.org/10.1093/nar/gkaa1140.

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Abstract HK022 coliphage site-specific recombinase Integrase (Int) can catalyze integrative site-specific recombination and recombinase-mediated cassette exchange (RMCE) reactions in mammalian cell cultures. Owing to the promiscuity of the 7 bp overlap sequence in its att sites, active ‘attB’ sites flanking human deleterious mutations were previously identified that may serve as substrates for RMCE reactions for future potential gene therapy. However, the wild type Int proved inefficient in catalyzing such RMCE reactions. To address this low efficiency, variants of Int were constructed and examined by integrative site-specific recombination and RMCE assays in human cells using native ‘attB’ sites. As a proof of concept, various Int derivatives have demonstrated successful RMCE reactions using a pair of native ‘attB’ sites that were inserted as a substrate into the human genome. Moreover, successful RMCE reactions were demonstrated in native locations of the human CTNS and DMD genes whose mutations are responsible for Cystinosis and Duchene Muscular Dystrophy diseases, respectively. This work provides a steppingstone for potential downstream therapeutic applications.
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Bateman, Jack R., et C. ting Wu. « A Simple Polymerase Chain Reaction-Based Method for the Construction of Recombinase-Mediated Cassette Exchange Donor Vectors ». Genetics 180, no 3 (14 septembre 2008) : 1763–66. http://dx.doi.org/10.1534/genetics.108.094508.

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Callesen, Morten M., Martin F. Berthelsen, Sira Lund, Annette C. Füchtbauer, Ernst-Martin Füchtbauer et Jannik E. Jakobsen. « Recombinase-Mediated Cassette Exchange (RMCE)-in Reporter Cell Lines as an Alternative to the Flp-in System ». PLOS ONE 11, no 8 (19 août 2016) : e0161471. http://dx.doi.org/10.1371/journal.pone.0161471.

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Ramachandra, Chrishan J. A., Mohammad Shahbazi, Timothy W. X. Kwang, Yukti Choudhury, Xiao Ying Bak, Jing Yang et Shu Wang. « Efficient recombinase-mediated cassette exchange at the AAVS1 locus in human embryonic stem cells using baculoviral vectors ». Nucleic Acids Research 39, no 16 (17 juin 2011) : e107-e107. http://dx.doi.org/10.1093/nar/gkr409.

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Shin, Seunghyeon, Su Hyun Kim, Jae Seong Lee et Gyun Min Lee. « Streamlined Human Cell-Based Recombinase-Mediated Cassette Exchange Platform Enables Multigene Expression for the Production of Therapeutic Proteins ». ACS Synthetic Biology 10, no 7 (16 juin 2021) : 1715–27. http://dx.doi.org/10.1021/acssynbio.1c00113.

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Di Domenico, Alexandra I., Ioannis Christodoulou, Steve C. Pells, Jim McWhir et Alison J. Thomson. « Sequential Genetic Modification of the hprt Locus in Human ESCs Combining Gene Targeting and Recombinase-Mediated Cassette Exchange ». Cloning and Stem Cells 10, no 2 (juin 2008) : 217–30. http://dx.doi.org/10.1089/clo.2008.0016.

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Baumann, Martina, Elisabeth Gludovacz, Natalie Sealover, Scott Bahr, Henry George, Nan Lin, Kevin Kayser et Nicole Borth. « Preselection of recombinant gene integration sites enabling high transcription rates in CHO cells using alternate start codons and recombinase mediated cassette exchange ». Biotechnology and Bioengineering 114, no 11 (17 août 2017) : 2616–27. http://dx.doi.org/10.1002/bit.26388.

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Takata, Yuki, Saki Kondo, Naoki Goda, Yumi Kanegae et Izumu Saito. « Comparison of efficiency between FLPe and Cre for recombinase-mediated cassette exchange in vitro and in adenovirus vector production ». Genes to Cells 16, no 7 (24 juin 2011) : 765–77. http://dx.doi.org/10.1111/j.1365-2443.2011.01526.x.

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Jakobsen, Jannik Ejnar, Marianne G. Johansen, Mette Schmidt, Frederik Dagnæs-Hansen, Karen Dam, Anders Gunnarsson, Ying Liu et al. « Generation of minipigs with targeted transgene insertion by recombinase-mediated cassette exchange (RMCE) and somatic cell nuclear transfer (SCNT) ». Transgenic Research 22, no 4 (31 octobre 2012) : 709–23. http://dx.doi.org/10.1007/s11248-012-9671-6.

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Li, Zhongsen, Bryan P. Moon, Aiqiu Xing, Zhan-Bin Liu, Richard P. McCardell, Howard G. Damude et S. Carl Falco. « Stacking Multiple Transgenes at a Selected Genomic Site via Repeated Recombinase-Mediated DNA Cassette Exchanges ». Plant Physiology 154, no 2 (18 août 2010) : 622–31. http://dx.doi.org/10.1104/pp.110.160093.

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