Littérature scientifique sur le sujet « Quantitative live-imaging »
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Articles de revues sur le sujet "Quantitative live-imaging"
Tany, Ryosuke, Yuhei Goto, Yohei Kondo et Kazuhiro Aoki. « Quantitative live-cell imaging of GPCR downstream signaling dynamics ». Biochemical Journal 479, no 8 (21 avril 2022) : 883–900. http://dx.doi.org/10.1042/bcj20220021.
Texte intégralGrossmann, Guido, Melanie Krebs, Alexis Maizel, Yvonne Stahl, Joop E. M. Vermeer et Thomas Ott. « Green light for quantitative live-cell imaging in plants ». Journal of Cell Science 131, no 2 (20 décembre 2017) : jcs209270. http://dx.doi.org/10.1242/jcs.209270.
Texte intégralYoun, Yeoan, Yongjae Lee, Gloria W. Lau et Paul R. Selvin. « Quantitative DNA-paint imaging of AMPA receptors in live neurons ». Biophysical Journal 121, no 3 (février 2022) : 141a. http://dx.doi.org/10.1016/j.bpj.2021.11.2028.
Texte intégralWoehler, Andrew. « Simultaneous Quantitative Live Cell Imaging of Multiple FRET-Based Biosensors ». PLoS ONE 8, no 4 (16 avril 2013) : e61096. http://dx.doi.org/10.1371/journal.pone.0061096.
Texte intégralLambert, Talley J., et Jennifer C. Waters. « Choosing a Fluorescence Microscopy Imaging Modality for Live Quantitative Experiments ». Microscopy and Microanalysis 20, S3 (août 2014) : 2122–23. http://dx.doi.org/10.1017/s1431927614012343.
Texte intégralTrivedi, Vikas, Yuwei Li, Thai V. Truong, David Koos, Chuong Cheng-Ming, Rex Moats et Scott E. Fraser. « How Embryonic Cartilage Grows : Insights Gained from Quantitative Live Imaging ». Biophysical Journal 106, no 2 (janvier 2014) : 575a. http://dx.doi.org/10.1016/j.bpj.2013.11.3188.
Texte intégralBurgess, Andrew, Thierry Lorca et Anna Castro. « Quantitative Live Imaging of Endogenous DNA Replication in Mammalian Cells ». PLoS ONE 7, no 9 (20 septembre 2012) : e45726. http://dx.doi.org/10.1371/journal.pone.0045726.
Texte intégralRudkouskaya, Alena, Nattawut Sinsuebphon, Jamie Ward, Kate Tubbesing, Xavier Intes et Margarida Barroso. « Quantitative imaging of receptor-ligand engagement in intact live animals ». Journal of Controlled Release 286 (septembre 2018) : 451–59. http://dx.doi.org/10.1016/j.jconrel.2018.07.032.
Texte intégralPlant, Anne L., Michael Halter et Jeffrey Stinson. « Probing pluripotency gene regulatory networks with quantitative live cell imaging ». Computational and Structural Biotechnology Journal 18 (2020) : 2733–43. http://dx.doi.org/10.1016/j.csbj.2020.09.025.
Texte intégralSengupta, Kheya, Eric Moyen, Magali Macé, Anne-Marie Benoliel, Anne Pierres, Frank Thibaudau, Laurence Masson, Laurent Limozin, Pierre Bongrand et Margrit Hanbücken. « Large-Scale Ordered Plastic Nanopillars for Quantitative Live-Cell Imaging ». Small 5, no 4 (20 février 2009) : 449–53. http://dx.doi.org/10.1002/smll.200800836.
Texte intégralThèses sur le sujet "Quantitative live-imaging"
Kim, Jang-Mi. « Quantitative live imaging analysis of proneural factor dynamics during lateral inhibition in Drosophila ». Electronic Thesis or Diss., Sorbonne université, 2022. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2022SORUS585.pdf.
Texte intégralLateral inhibition by Notch is a conserved mechanism that regulates the formation of regular patterns of cell fates1. In many tissues, intercellular Delta-Notch signaling coordinates in time and space binary fate decisions thought to be stochastic. In the context of sensory organ development in Drosophila, it has been proposed that fate symmetry breaking between equipotent cells relies on random fluctuations in the level of Delta/Notch2 (or one of their upstream regulators, e.g. YAP1 in the mouse gut3), with small differences being amplified and stabilized to generate distinct fates. Notch-mediated stochastic fate choices may also be biased by intrinsic, i.e. cell history4, or extrinsic factors. Although lateral inhibition has been extensively studied in many developmental contexts, a detailed in vivo analysis of fate and signaling dynamics is still lacking. Here, we used a quantitative live imaging approach to study the dynamics of sensory organ fate specification in the Drosophila abdomen. The accumulation of the transcription factor Scute (Sc), a key regulator of sensory organ formation in the abdomen, was used as a proxy to monitor proneural competence and SOP fate acquisition in developing pupae expressing GFP-tagged Sc. We generated high spatial and temporal resolution movies and segmented/tracked all nuclei using a custom-made pipeline. This allowed us to quantitatively study Sc dynamics in all cells. Having defined a fate difference index (FDI), we found that symmetry breaking can be detected early, when cells expressed very low and heterogeneous levels of Sc. We also observed rare cases of late fate resolution, e.g. when two cells close to each other accumulate high levels of GFP-Scute before being pulled away from each other. Interestingly, we did not observe a rapid decrease in GFP-Sc levels in non-selected cells right after symmetry breaking. Also, the rate of change of FDI values after symmetry breaking appeared to positively correlate with cell-to-cell heterogeneity in Sc levels. Whether increased heterogeneity is causally linked to symmetry breaking remains to be tested. We next addressed if this stochastic fate decision is biased by birth order (as proposed in the context of the AC/VU decision in worms4) or by the size and geometry of cell-cell contacts (as modeling suggested5). We found that neither appeared to significantly influence Notch-mediated binary fate decisions in the Drosophila abdomen. In conclusion, our live imaging data provide a detailed analysis of proneural dynamics during lateral inhibition in Drosophila
Boni, Andrea [Verfasser], et Jan [Akademischer Betreuer] Ellenberg. « Inner nuclear membrane protein targeting studied by quantitative live cell imaging and RNAi screening / Andrea Boni ; Betreuer : Jan Ellenberg ». Heidelberg : Universitätsbibliothek Heidelberg, 2016. http://d-nb.info/1180608046/34.
Texte intégralMonypenny, James Edward. « Development of quantitative live cell imaging techniques and their applications in the study of inter-cellular communication and Sarcoma cell motility ». Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.406165.
Texte intégralLu, Yi-Ju [Verfasser], et Jane [Akademischer Betreuer] Parker. « Live-cell imaging reveals subcellular localization of plant membrane compartments during oomycete infections and quantitative high-throughput imaging identifies endocytic trafficking mutants / Yi-Ju Lu. Gutachter : Jane Parker ». Köln : Universitäts- und Stadtbibliothek Köln, 2012. http://d-nb.info/1038225981/34.
Texte intégralTorrano, Adriano de Andrade [Verfasser], et CHRISTOPH [Akademischer Betreuer] BRAEUCHLE. « Quantitative live-cell imaging studies on the biological effects of nanoparticles at the cellular level / Adriano de Andrade Torrano. Betreuer : Christoph Bräuchle ». München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2015. http://d-nb.info/110469798X/34.
Texte intégralChamiolo, Jasmine. « Verbesserte FIT-Sonden für die selektive und quantitative RNA-Visualisierung in lebenden Zellen ». Doctoral thesis, Humboldt-Universität zu Berlin, 2020. http://dx.doi.org/10.18452/20889.
Texte intégralIn this work forced Intercalation (FIT) probes, developed by Seitz et al. were used for the mRNA characterization in living cells. The synthesis of improved FIT probes as well as the systematic study on the uptake of FIT probes by living Flp-In™ 293 T-REx™ cells was performed. Therefore FIT probe conjugates/aggregates as well as commercially available reagents, e.g. palmitic acid and the pore-forming enzyme Streptolysin-O were investigated under various conditions. Furthermore, the transfection was tested using an electroporator. The optimal transfection condition for the introduction of DNA and PNA FIT probes into Flp-In™ 293 T-REx™ cells was achieved using Streptolysin-O. Multicolor live cell imaging with the simultaneous use of three different FIT probes (BO, TO and QB) against three different target sequences was performed successfully. In addition, FIT probes were used for the differentiation between T cell lines. A competitive hybridization experiment with cells confirmed the specific fluorescence emission of the probes. Further studies with two cell lines and TO-FIT probes as well as terminal Cy7-labeled TO-FIT probes showed an increased TO emission in the presence of the complementary TCR mRNA target sequence in the cells. A second detection channel of the terminal Cy7 dye provided the advantage of comparing the Cy7- and TO-intensity ratio, thereby making it easier to exclude signals from unbound probe. This enabled the specific tagging of t cells. This was followed by the synthesis of QB-DNA-based FIT probes for the use in various biological applications e.g. as a pan selective marker for Influenza A infection. Moreover, the synthesis and evaluation of new dyes with an absorption maximum at 590/596 nm was performed. The incorporation of a cyclic PNA monomer next to the TO dye has also been realized to improve responsiveness and brightness in PNA-FIT probes.
Verkhedkar, Ketki Dinesh. « Quantitative Analysis of DNA Repair and p53 in Individual Human Cells ». Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10660.
Texte intégralWang, Renjie. « Quantitative analysis of chromatin dynamics and nuclear geometry in living yeast cells ». Thesis, Toulouse 3, 2016. http://www.theses.fr/2016TOU30122/document.
Texte intégralChromosome high-order architecture has been increasingly studied over the last decade thanks to technological breakthroughs in imaging and in molecular biology. It is now established that structural organization of the genome is a key determinant in all aspects of genomic transactions. Although several models have been proposed to describe the folding of chromosomes, the physical principles governing their organization are still largely debated. Nucleus is the cell’s compartment in which chromosomal DNA is confined. Geometrical constrains imposed by nuclear confinement are expected to affect high-order chromatin structure. However, the quantitative measurement of the influence of the nuclear structure on the genome organization is unknown, mostly because accurate nuclear shape and size determination is technically challenging. This thesis was organized along two axes: the first aim of my project was to study the dynamics and physical properties of chromatin in the S. cerevisiae yeast nucleus. The second objective I had was to develop techniques to detect and analyze the nuclear 3D geomtry with high accuracy. Ribosomal DNA (rDNA) is the repetitive sequences which clustered in the nucleolus in budding yeast cells. First, I studied the dynamics of non-rDNA and rDNA in exponentially growing yeast cells. The motion of the non-rDNA could be modeled as a two-regime Rouse model. The dynamics of rDNA was very different and could be fitted well with a power law of scaling exponent ~0.7. Furthermore, we compared the dynamics change of non-rDNA in WT strains and temperature sensitive (TS) strains before and after global transcription was actived. The fluctuations of non-rDNA genes after transcriptional inactivation were much higher than in the control strain. The motion of the chromatin was still consistent with the Rouse model. We propose that the chromatin in living cells is best modeled using an alternative Rouse model: the “branched Rouse polymer”. Second, we developed “NucQuant”, an automated fluorescent localization method which accurately interpolates the nuclear envelope (NE) position in a large cell population. This algorithm includes a post-acquisition correction of the measurement bias due to spherical aberration along Z-axis. “NucQuant” can be used to determine the nuclear geometry under different conditions. Combined with microfluidic technology, I could accurately estimate the shape and size of the nuclei in 3D along entire cell cycle. “NucQuant” was also utilized to detect the distribution of nuclear pore complexes (NPCs) clusters under different conditions, and revealed their non-homogeneous distribution. Upon reduction of the nucleolar volume, NPCs are concentrated in the NE flanking the nucleolus, suggesting a physical link between NPCs and the nucleolar content. In conclusion, we have further explored the biophysical properties of the chromatin, and proposed that chromatin in the nucleoplasm can be modeled as "branched Rouse polymers". Moreover, we have developed “NucQuant”, a set of computational tools to facilitate the study of the nuclear shape and size. Further analysis will be required to reveal the links between the nucleus geometry and the chromatin dynamics
Kawahira, Naofumi. « Quantitative analysis of 3D tissue deformation reveals key cellular mechanism associated with initial heart looping ». Kyoto University, 2020. http://hdl.handle.net/2433/254507.
Texte intégralChen, Huiyi. « System-Wide Studies of Gene Expression in Escherichia coli by Fluorescence Microscopy and High Throughput Sequencing ». Thesis, Harvard University, 2011. http://dissertations.umi.com/gsas.harvard:10044.
Texte intégralChapitres de livres sur le sujet "Quantitative live-imaging"
Le Marois, Alix, et Klaus Suhling. « Quantitative Live Cell FLIM Imaging in Three Dimensions ». Dans Advances in Experimental Medicine and Biology, 31–48. Cham : Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-67358-5_3.
Texte intégralSchöler, Ulrike, Anna-Lena Merten, Sebastian Schürmann et Oliver Friedrich. « Quantitative Live-Cell Ca2+ Imaging During Isotropic Cell Stretch ». Dans Methods in Molecular Biology, 155–76. New York, NY : Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3052-5_10.
Texte intégralKemper, Björn, et Jürgen Schnekenburger. « Digital Holographic Microscopy for Quantitative Live Cell Imaging and Cytometry ». Dans Advanced Optical Flow Cytometry, 211–37. Weinheim, Germany : Wiley-VCH Verlag GmbH & Co. KGaA, 2011. http://dx.doi.org/10.1002/9783527634286.ch8.
Texte intégralRakymzhan, Asylkhan, Helena Radbruch et Raluca A. Niesner. « Quantitative Imaging of Ca2+ by 3D–FLIM in Live Tissues ». Dans Advances in Experimental Medicine and Biology, 135–41. Cham : Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-67358-5_9.
Texte intégralKaminski, Clemens F., Eric J. Rees et Gabriele S. Kaminski Schierle. « A Quantitative Protocol for Intensity-Based Live Cell FRET Imaging ». Dans Methods in Molecular Biology, 445–54. Totowa, NJ : Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-649-8_19.
Texte intégralMarquet, Pierre, Benjamin Rappaz et Nicolas Pavillon. « Quantitative Phase-Digital Holographic Microscopy : A New Modality for Live Cell Imaging ». Dans New Techniques in Digital Holography, 169–217. Hoboken, NJ, USA : John Wiley & Sons, Inc., 2015. http://dx.doi.org/10.1002/9781119091745.ch5.
Texte intégralJelcic, Mark, Balázs Enyedi et Philipp Niethammer. « Quantitative Imaging of Endogenous and Exogenous H2O2 Gradients in Live Zebrafish Larvae ». Dans Methods in Molecular Biology, 283–99. New York, NY : Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9424-3_17.
Texte intégralKemper, Björn, Patrik Langehanenberg, Sebastian Kosmeier, Frank Schlichthaber, Christian Remmersmann, Gert von Bally, Christina Rommel, Christian Dierker et Jürgen Schnekenburger. « Digital Holographic Microscopy : Quantitative Phase Imaging and Applications in Live Cell Analysis ». Dans Handbook of Coherent-Domain Optical Methods, 215–57. New York, NY : Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4614-5176-1_6.
Texte intégralAmin, Viren, Mercedes Izquirdo, Doyle Wilson, Gene Rouse et Ronald Roberts. « Ultrasonic Evaluation of Quality Attributes in Live Beef Animals Using Real-Time B-Mode Ultrasound Imaging ». Dans Review of Progress in Quantitative Nondestructive Evaluation, 1329–34. Boston, MA : Springer US, 1996. http://dx.doi.org/10.1007/978-1-4613-0383-1_173.
Texte intégralBadal, Kerriann, Yibo Zhao, Kyle E. Miller et Sathyanarayanan V. Puthanveettil. « Live Imaging and Quantitative Analysis of Organelle Transport in Sensory Neurons of Aplysia Californica ». Dans Methods in Molecular Biology, 23–48. New York, NY : Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-1990-2_2.
Texte intégralActes de conférences sur le sujet "Quantitative live-imaging"
Wax, Adam. « Phase imaging of mechanical properties of live cells (Conference Presentation) ». Dans Quantitative Phase Imaging III, sous la direction de Gabriel Popescu et YongKeun Park. SPIE, 2017. http://dx.doi.org/10.1117/12.2255903.
Texte intégralChoi, Wonshik, Yongjin Sung, Yongkeun Park, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari et Michael S. Feld. « Quantitative live cell imaging with tomographic phase microscopy ». Dans Novel Techniques in Microscopy. Washington, D.C. : OSA, 2009. http://dx.doi.org/10.1364/ntm.2009.ntua1.
Texte intégralShu, Xin, Yi Zhang, Mengxuan Niu, Wei Luo et Renjie Zhou. « Compact and ease-of-use quantitative phase microscopy for real-time live-cell imaging ». Dans Quantitative Phase Imaging VIII, sous la direction de Gabriel Popescu, YongKeun Park et Yang Liu. SPIE, 2022. http://dx.doi.org/10.1117/12.2610489.
Texte intégralSlabý, Tomáš, Aneta Křížová, Martin Lošt'ák, Jana Čolláková, Veronika Jůzová, Pavel Veselý et Radim Chmelík. « Coherence-controlled holographic microscopy for live-cell quantitative phase imaging ». Dans SPIE BiOS, sous la direction de Gabriel Popescu et YongKeun Park. SPIE, 2015. http://dx.doi.org/10.1117/12.2080128.
Texte intégralKemper, Bjorn. « Multi-parameter quantitative live cell imaging with digital holographic microscopy ». Dans 2013 IEEE Photonics Conference (IPC). IEEE, 2013. http://dx.doi.org/10.1109/ipcon.2013.6656487.
Texte intégralSaintoyant, Anaïs, Sherazade Aknoun, Fabrice Valentino, Antoine Federici et Benoit Wattellier. « High-Definition Quantitative Phase Imaging System applied to live cell samples ». Dans Digital Holography and Three-Dimensional Imaging. Washington, D.C. : OSA, 2019. http://dx.doi.org/10.1364/dh.2019.th4a.6.
Texte intégralPandiyan, Vimal Prabhu, et Renu John. « Quantitative Phase imaging of Live Yeast cells using Digital Holographic Microscopy ». Dans International Conference on Fibre Optics and Photonics. Washington, D.C. : OSA, 2014. http://dx.doi.org/10.1364/photonics.2014.s5a.4.
Texte intégralZuo, Chao, Yefeng Shu et Jiasong Sun. « Adaptive optical quantitative phase imaging with annular illumination Fourier ptychographic microscopy ». Dans Digital Holography and Three-Dimensional Imaging. Washington, D.C. : Optica Publishing Group, 2022. http://dx.doi.org/10.1364/dh.2022.w7a.3.
Texte intégralLing, Zhi, Keyi Han, Wenhao Liu, Xuanwen Hua et Shu Jia. « Volumetric Live-Cell Autofluorescence Imaging Using Fourier Light-Field Microscopy ». Dans Frontiers in Optics. Washington, D.C. : Optica Publishing Group, 2023. http://dx.doi.org/10.1364/fio.2023.jm4a.79.
Texte intégralPandiyan, Vimal Prabhu, Kedar Khare et Renu John. « High resolution quantitative phase imaging of live cells with constrained optimization approach ». Dans SPIE BiOS, sous la direction de Gabriel Popescu et YongKeun Park. SPIE, 2016. http://dx.doi.org/10.1117/12.2209289.
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