Articles de revues : « Psoraleni »

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Diekmann, Janina, Isabell Theves, Kristoffer A. Thom et Peter Gilch. « Tracing the Photoaddition of Pharmaceutical Psoralens to DNA ». Molecules 25, n^o 22 (10 novembre 2020) : 5242. http://dx.doi.org/10.3390/molecules25225242.

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The psoralens 8-methoxypsoralen (8-MOP), 4,5′,8-trimethylpsoralen (TMP) and 5-methoxypsoralen (5-MOP) find clinical application in PUVA (psoralen + UVA) therapy. PUVA treats skin diseases like psoriasis and atopic eczema. Psoralens target the DNA of cells. Upon photo-excitation psoralens bind to the DNA base thymine. This photo-binding was studied using steady-state UV/Vis and IR spectroscopy as well as nanosecond transient UV/Vis absorption. The experiments show that the photo-addition of 8-MOP and TMP involve the psoralen triplet state and a biradical intermediate. 5-MOP forms a structurally different photo-product. Its formation could not be traced by the present spectroscopic technique.

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Jamalis, Joazaizulfazli, Faten Syahira Mohamed Yusof, Subhash Chander, Roswanira Abd Wahab, Deepak P. Bhagwat, Murugesan Sankaranarayanan, Faisal Almalki et Taibi Ben Hadda. « Psoralen Derivatives : Recent Advances of Synthetic Strategy and Pharmacological Properties ». Anti-Inflammatory & ; Anti-Allergy Agents in Medicinal Chemistry 19, n^o 3 (3 septembre 2020) : 222–39. http://dx.doi.org/10.2174/1871523018666190625170802.

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Psoralen or furocoumarin is a linear three ring heterocyclic compound. Psoralens are planar, tricyclic compounds, consisting of a furan ring fused to a coumarin moiety. Psoralen has been known for a wide spectrum of biological activities, spanning from cytotoxic, photosensitizing, insecticidal, antibacterial to antifungal effect. Thus, several structural changes were introduced to explore the role of specific positions with respect to the biological activity. Convenient approaches utilized for the synthesis of psoralen skeleton can be categorized into two parts: (i) the preparation of the tricyclic ring system from resorcinol, (ii) the exocyclic modification of the intact ring system. Furthermore, although psoralens have been used in diverse ways, we mainly focus in this work on their clinical utility for the treatment of psioraisis, vitiligo and skin-related disorder.

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Da Silva, Vinicius Barreto, Daniel Fábio Kawano, Ivone Carvalho, Edemilson Cardoso Conceição, Osvaldo Freitas et Carlos Henrique Tomich de Paula Silva. « Psoralen and Bergapten : In Silico Metabolism and Toxicophoric Analysis of Drugs Used to Treat Vitiligo ». Journal of Pharmacy & ; Pharmaceutical Sciences 12, n^o 3 (9 décembre 2009) : 378. http://dx.doi.org/10.18433/j3w01d.

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PURPOSE: to discuss the contribution of psoralen and bergapten metabolites on psoralens toxicity. METHODS: Computational chemistry prediction of metabolic reactions and toxicophoric groups based on the expert systems Derek and Meteor. RESULTS: a total of 15 metabolites were suggested for both psoralen and bergapten based on phase 1 and 2 biotransformations until the 3rd generation. Five toxicophoric substructures were shared among psoralen, bergapten and their corresponding metabolites; one toxicophoric marker (resorcinol) was only identified in bergapten and its biotransformation products. CONCLUSION: Although the toxic effects of psoralens are well known and documented, there is little information concerning the role of their metabolites in this process. We believe this work add to the knowledge of which molecular substructures are relevant to the process of metabolism and toxicity induction, thus guiding the search and development of more effective and less toxic drugs to treat vitiligo.

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Patel, Kanika, Vikas Kumar, Amita Verma, Mahfoozur Rahman et Dinesh Kumar Patel. « Health Benefits of Furanocoumarins ‘Psoralidin’ An Active Phytochemical of Psoralea corylifolia : The Present, Past and Future Scenario ». Current Bioactive Compounds 15, n^o 4 (4 juillet 2019) : 369–76. http://dx.doi.org/10.2174/1573407214666180511153438.

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Background: Phytoconstituents of fruits, vegetables, spice and herbs are important for human health. Phytoconstituents play an important role in the growth, development, defensive mechanism, colors, odour and flavors of plants. Psoralea corylifolia Linn (P. corylifolia) commonly known as ‘Bakuchi’ belongs to Leguminosae family widely distributed in China and Southeastern Asian countries. Methods: Various electronic databases such as PubMed, Science Direct, Scopus and Google were searched to collect the data of the present review. Further, all the collected information was categorized into different section as per the aim of the paper. Moreover, analyzed data were also presented in the graphical abstract. Results: Seventy-two research and review papers have been collected and were included in the present review. Nineteen papers contained general information’s of P. corylifolia, Psoralens and psoralidin whereas thirty paper data were presented in the pharmacological activities sections and remaining in the Analytical tools and discussion section. From these databases, we can say that P. corylifolia possesses antibacterial, anti-inflammatory, antifungal, antioxidant, antiflarial, estrogenic, antitumour, and immunomodulatory activities. Psoralens are mainly used in the treatment of psoriasis, vitiligo and dermatitis. Topical or oral psoralens followed by UVA radiation exposure are one of the best treatments of leucoderma. Psoralidin exhibits antioxidant, anti-apoptotic, anti-inflammatory and antitumor activities. Conclusion: This review summarizes an overview of P. corylifolia, psoralen and its derivative psoralidin with respects to their pharmacological activities, medicinal uses, biological activities and bioanalytical aspects. These data will be helpful in the translation of information from traditional to the modern medicine of psoralidin.

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Huseyn A. Abiyev et Khayala R. Mammadova. « Effects of α-tocopherol on phototoxicity of 8 - methoxypsoralene ». Magna Scientia Advanced Biology and Pharmacy 2, n^o 1 (30 mars 2021) : 01–08. http://dx.doi.org/10.30574/msabp.2021.2.1.0005.

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Effects of singlet oxygen extinguisher α-tocopherol on combined effects of psoralens and UV on erythema photosensitized with 8-methoxypsoralene were investigated. The leading role of the reactions of photocompounds in induction of PUVA - erythema is considered. A characteristic feature of biologically active psoralens is the high value of quantum output of intercombination conversion, due to which, after absorption of light quants, a significant number of psoralen molecules are formed in a triplet excited state. Psoralen derivatives differ greatly in their ability to photosensitize the skin. In the example of 8-psoralens, a correlation was found between the ability to induce erythema by photocompound to double-spiral DNA in vitro. A clear correlation was identified between the ability to photosensitize erythema and form diadducts. Α-tocopherol has been shown to protect the skin from PUVA erythema if presents during irradiation. In the case of application of α-tocopherol after irradiation, the inhibitory effect was not exhibited.

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Zhao, Gen-hua, Cui-ping Yan, Zi-sheng Xu, Qian-qian Gao, Zhi-peng Chen et Wei-dong Li. « The Effect of Salt-Processed Psoralea corylifolia on Generative Organ Targeting ». Journal of Analytical Methods in Chemistry 2016 (2016) : 1–12. http://dx.doi.org/10.1155/2016/7484202.

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Psoralen and isopsoralen are two isomers and main effective components within Psoralea corylifolia. In order to investigate the salt-processing effect on tissue distribution characters of psoralen and isopsoralen, a sensitive and accurate ultrahigh pressure liquid chromatographic tandem mass spectrometric (UHPLC-MS/MS) method has been developed and validated for simultaneous determination of the 2 components in rats’ tissues after administration of the extracts that came from either crude or salt-processed Psoralea corylifolia L. Data displayed that both areas under the curve (AUC) of psoralen and isopsoralen from salt-processed scurfpea fruit group were significantly increased compared with that of the crude herb group, especially in heart (p<0.05), ovary, and testes (p<0.001). Though the RE and RCmax of psoralen and isopsoralen in all of the investigated organs were over 1.0, generative organs kept the maximum value. The experiment manifested that salt-processing of scurfpea fruit can increase the distribution of psoralen and isopsoralen to generative organs, heart and spleen, and the distribution of psoralen and isopsoralen to generative organs is significantly higher compared to heart and spleen (p<0.01). Results indicate that salt-processing of scurfpea fruit can significantly increase the distribution of psoralen and isopsoralen to generative organs.

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Chen, Yuling, Rong Wang et Zilin Chen. « Sensitive determination of psoralen and isopsoralen in Fructus Psoraleae by online solid phase microextraction with a porphyrin-based porous organic polymer modified capillary ». Analytical Methods 11, n^o 1 (2019) : 29–35. http://dx.doi.org/10.1039/c8ay01885e.

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Thomas, D. C., D. L. Svoboda, J. M. Vos et T. A. Kunkel. « Strand specificity of mutagenic bypass replication of DNA containing psoralen monoadducts in a human cell extract. » Molecular and Cellular Biology 16, n^o 5 (mai 1996) : 2537–44. http://dx.doi.org/10.1128/mcb.16.5.2537.

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Psoralens are mutagenic compounds of vegetable origin that are used as photosensitizing agents in the treatment of various skin diseases, blood cell cancer, and autoimmune disorders. To study the mechanism of mutagenicity of psoralens in humans, we examined the efficiency and fidelity of simian virus 40 origin-dependent replication in a human cell extract of M13mp2 DNA randomly treated with the psoralen derivative 4'-hydroxymethyl-4,5',8-trimethyl psoralen plus UVA irradiation. Replication of DNA treated with variable amounts of 4'-hydroxymethyl-4,5',8-trimethyl psoralen and a fixed UVA fluence was inhibited in a concentration-dependent manner. However, covalently closed monomer-length circular replication products were observed. Product analysis by renaturing agarose gel electrophoresis after cross-linking with 250- to 280-nm UV light indicated that approximately 1 of 9 psoralen monoadducts was bypassed during in vitro replication. Introduction of product DNA into Escherichia coli to score replication errors in the lacZalpha reporter gene demonstrated that replication of the damaged DNA was more mutagenic than was replication of undamaged DNA. Sequence analysis of lacZ mutants revealed that damage-dependent replication errors were predominantly T.A-->C.G transitions, transversions at C.G base pairs, and deletions of single A.T base pairs, the last occurring most frequently in homopolymeric runs. A comparison of error specificities with two substrates having the replication origin asymmetrically placed on opposite sides of the mutational target suggests that the lagging-strand replication apparatus is less accurate than the leading-strand replication apparatus for psoralen monoadduct-dependent deletion errors. A model is proposed based on the preferential loopout of the monoadducted base from the strand that templates retrograde discontinuous synthesis.

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Grass, Joshua A., Derek J. Hei, Ken Metchette, George D. Cimino, Gary P. Wiesehahn, Laurence Corash et Lily Lin. « Inactivation of Leukocytes in Platelet Concentrates by Photochemical Treatment With Psoralen Plus UVA ». Blood 91, n^o 6 (15 mars 1998) : 2180–88. http://dx.doi.org/10.1182/blood.v91.6.2180.

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Abstract A photochemical treatment (PCT) process using a novel psoralen and long wavelength ultraviolet light (UVA, 320-400 nm) has been developed to inactivate bacteria and viruses in platelet concentrates. This study evaluated the efficacy of PCT for inactivation of leukocytes that contaminate platelet preparations. Three psoralens, 8-methoxypsoralen (8-MOP), 4′-aminomethyl 4,5′,8-trimethylpsoralen (AMT), and the novel psoralen S-59, were compared using the following four independent but complementary biological and molecular assays. (1) T-cell viability: Treatment with 150 μmol/L S-59 and 1.0 to 3.0 Joules/cm2 UVA inactivated >5.4 ± 0.3 log10 of T cells in full-sized single-donor plateletpheresis units. Using 1.0 Joule/cm2 UVA, the lowest dose of S-59, AMT and 8-MOP required to reduce the number of T cells to the limit of detection was 0.05 μmol/L, 1.0 μmol/L, and 10.0 μmol/L, respectively. (2) Cytokine synthesis: Treatment with 1.9 Joules/cm2 UVA and 150 μmol/L S-59 or AMT completely inhibited synthesis of the cytokine IL-8 by contaminating leukocytes during 5 days of platelet storage. After treatment with 75 μmol/L 8-MOP and 1.9 Joules/cm2 UVA, only low levels of IL-8 were detected. (3) Psoralen-DNA adduct formation: The combination of 1.9 Joules/cm2 UVA and 150 μmol/L S-59, AMT, or 8-MOP induced 12.0 ± 3.0, 6.0 ± 0.9, and 0.7 psoralen adducts per 1,000 bp DNA, respectively. (4) Replication competence: Polymerase chain reaction (PCR) amplification of small genomic DNA sequences (242-439 bp) after PCT was inhibited. The degree of PCR amplification inhibition correlated with the level of adduct formation (S-59 > AMT > 8-MOP). In contrast, 2,500 cGy gamma radiation, a dose that inactivates >5 log10 of T cells in blood products, had minimal effect on cytokine synthesis and did not induce sufficient DNA strand breaks to inhibit PCR amplification of the same small DNA sequences. These results demonstrate that leukocytes are sensitive to PCT with psoralens and among the psoralens tested S-59 is the most effective. Therefore, PCT has the potential to reduce the incidence of leukocyte-mediated adverse immune reactions associated with platelet transfusion.

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Grass, Joshua A., Derek J. Hei, Ken Metchette, George D. Cimino, Gary P. Wiesehahn, Laurence Corash et Lily Lin. « Inactivation of Leukocytes in Platelet Concentrates by Photochemical Treatment With Psoralen Plus UVA ». Blood 91, n^o 6 (15 mars 1998) : 2180–88. http://dx.doi.org/10.1182/blood.v91.6.2180.2180_2180_2188.

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A photochemical treatment (PCT) process using a novel psoralen and long wavelength ultraviolet light (UVA, 320-400 nm) has been developed to inactivate bacteria and viruses in platelet concentrates. This study evaluated the efficacy of PCT for inactivation of leukocytes that contaminate platelet preparations. Three psoralens, 8-methoxypsoralen (8-MOP), 4′-aminomethyl 4,5′,8-trimethylpsoralen (AMT), and the novel psoralen S-59, were compared using the following four independent but complementary biological and molecular assays. (1) T-cell viability: Treatment with 150 μmol/L S-59 and 1.0 to 3.0 Joules/cm2 UVA inactivated >5.4 ± 0.3 log10 of T cells in full-sized single-donor plateletpheresis units. Using 1.0 Joule/cm2 UVA, the lowest dose of S-59, AMT and 8-MOP required to reduce the number of T cells to the limit of detection was 0.05 μmol/L, 1.0 μmol/L, and 10.0 μmol/L, respectively. (2) Cytokine synthesis: Treatment with 1.9 Joules/cm2 UVA and 150 μmol/L S-59 or AMT completely inhibited synthesis of the cytokine IL-8 by contaminating leukocytes during 5 days of platelet storage. After treatment with 75 μmol/L 8-MOP and 1.9 Joules/cm2 UVA, only low levels of IL-8 were detected. (3) Psoralen-DNA adduct formation: The combination of 1.9 Joules/cm2 UVA and 150 μmol/L S-59, AMT, or 8-MOP induced 12.0 ± 3.0, 6.0 ± 0.9, and 0.7 psoralen adducts per 1,000 bp DNA, respectively. (4) Replication competence: Polymerase chain reaction (PCR) amplification of small genomic DNA sequences (242-439 bp) after PCT was inhibited. The degree of PCR amplification inhibition correlated with the level of adduct formation (S-59 > AMT > 8-MOP). In contrast, 2,500 cGy gamma radiation, a dose that inactivates >5 log10 of T cells in blood products, had minimal effect on cytokine synthesis and did not induce sufficient DNA strand breaks to inhibit PCR amplification of the same small DNA sequences. These results demonstrate that leukocytes are sensitive to PCT with psoralens and among the psoralens tested S-59 is the most effective. Therefore, PCT has the potential to reduce the incidence of leukocyte-mediated adverse immune reactions associated with platelet transfusion.

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Zobel, Alicja M., Stewart A. Brown et Raymond E. March. « Histological localization of psoralens in fruits of Psoralea bituminosa ». Canadian Journal of Botany 69, n^o 8 (1 août 1991) : 1673–78. http://dx.doi.org/10.1139/b91-212.

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Linear furanocoumarins were found on the surface of Psoralea bituminosa (Leguminosae) fruits in concentrations up to 1% of the total furanocoumarins. Most of the remainder was on the surface of the embryos and very young seedlings, with ca. 4% of the total furanocoumarins within the embryos, and very little in the seed coat and fruit. Psoralen was present in the highest concentration, over twice that of xanthotoxin. There was very little bergapten, whose distribution differed from that of the xanthotoxin and psoralen, ca. 20% being on the surface and the rest in dead tissues, i.e., fruit and seed covers. Evidence was obtained, through autofluorescence, that these coumarins are localized in discrete cells of the embryo, on the embryo surface, and on the fruit surface. Key words: Psoralea, Leguminosae, fruits, furanocoumarins, autofluorescence, histochemistry.

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Yu, Yingli, Pengli Wang, Ruili Yu, Jiaxi Lu, Miaomiao Jiang et Kun Zhou. « Long-Term Exposure of Psoralen and Isopsoralen Induced Hepatotoxicity and Serum Metabolites Profiles Changes in Female Rats ». Metabolites 9, n^o 11 (2 novembre 2019) : 263. http://dx.doi.org/10.3390/metabo9110263.

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Pre-clinical safety evaluation of traditional medicines is imperative because of the universality of drug-induced adverse reactions. Psoralen and isopsoralen are the major active molecules and quality-control components of a traditional herbal medicine which is popularly used in Asia, Fructus Psoraleae. The purpose of this study is to assess the long-term effects of psoralen and isopsoralen with low levels on the biochemical parameters and metabolic profiles of rats. Three doses (14, 28, and 56 mg/kg) of psoralen and one dose (28 mg/kg) of isopsoralen were administered to rats over 12 weeks. Blood and selected tissue samples were collected and analyzed for hematology, serum biochemistry, and histopathology. Metabolic changes in serum samples were detected via proton nuclear magnetic resonance (1H-NMR) spectroscopy. We found that psoralen significantly changed the visceral coefficients, blood biochemical parameters, and histopathology, and isopsoralen extra influenced the hematological index. Moreover, psoralen induced remarkable elevations of forvaline, isoleucine, isobutyrate, alanine, acetone, pyruvate, glutamine, citrate, unsaturated lipids, choline, creatine, phenylalanine, and 4-hydroxybenzoate, and significant reductions of ethanol and dimethyl sulfone. Isopsoralen only induced a few remarkable changes of metabolites. These results suggest that chronic exposure to low-level of psoralen causes a disturbance in alanine metabolism, glutamate metabolism, urea cycle, glucose-alanine cycle, ammonia recycling, glycine, and serine metabolism pathways. Psoralen and isopsoralen showed different toxicity characteristics to the rats.

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Shi, Mengjun, Juanjuan Zhang, Cunyu Liu, Yiping Cui, Changqin Li, Zhenhua Liu et Wenyi Kang. « Ionic Liquid-Based Ultrasonic-Assisted Extraction to Analyze Seven Compounds in Psoralea Fructus Coupled with HPLC ». Molecules 24, n^o 9 (30 avril 2019) : 1699. http://dx.doi.org/10.3390/molecules24091699.

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Psoralea Fructus is widely used in traditional Chinese medicine (TCM), and the content of psoralen, isopsoralen, neobavaisoflavone, bavachin, psoralidin, isobavachalcone, and bavachinin A is the main quality control index of Psoralea Fructus because of its clinical effects. Thus, a fast and environmentally-benign extraction method of seven compounds in Psoralea Fructus is necessary. In this work, an ionic liquid-based ultrasonic-assisted method (ILUAE) for the extraction of seven compounds from Psoralea Fructus was proposed. Several ILs of different types and parameters, including the concentration of ILs, concentration of ethanol (EtOH), solid–liquid ratio, particle size, ultrasonic time, centrifugal speed, and ultrasonic power, were optimized by the Placket–Burman (PB) design and Box–Behnken response surface analysis. Under this optimal condition, the total extraction yield of the seven compounds in Psoralea Fructus was 18.90 mg/g, and significantly greater than the conventional 75% EtOH solvent extraction.

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Jeong, Mijeong, Thao Hong, Kangwoo Lee, Hyunjung Hwangbo, Minjung Kim, Wenwen Ma et Michael Zahn. « HPLC Method for Simultaneous Quantification of Bakuchiol and Minor Furocoumarins in Bakuchiol Extract from Psoralea corylifolia ». Journal of AOAC INTERNATIONAL 98, n^o 4 (1 juillet 2015) : 902–6. http://dx.doi.org/10.5740/jaoacint.14-228.

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Abstract A simple, sensitive, and rapid HPLC method was developed to analyze bakuchiol and two furocoumarins (psoralen and angelicin) simultaneously in bakuchiol extracts from Psoralea corylifolia seeds. The analysis was performed within 30 min on a phenyl-hexyl column using gradient elution with a mobile phase composed of water and methanol with UV detection at 260 nm for bakuchiol and 246 nm for psoralen and angelicin. The method was validated with respect to linearity (r2 > 0.99 for all components), accuracy (>95% for all components), and precision (<2% RSD for both interday and intraday). Sensitivity of impurity detection in the sample was achieved as low as 0.36 and 0.31 μg/mL for psoralen and angelicin, respectively. Therefore, the method is suitable for QC of P. corylifolia extracts and bakuchiol related samples.

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Devor, D. C., A. K. Singh, R. J. Bridges et R. A. Frizzell. « Psoralens : novel modulators of Cl- secretion ». American Journal of Physiology-Cell Physiology 272, n^o 3 (1 mars 1997) : C976—C988. http://dx.doi.org/10.1152/ajpcell.1997.272.3.c976.

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We evaluated effects of psoralens on Cl- secretion (short-circuit current, I(sc)) across T84 monolayers. Methoxsalen failed to increase I(sc). Several observations suggest that psoralens open cystic fibrosis transmembrane conductance regulator Cl- channels. 1) After activation of the Ca2+-dependent basolateral membrane K+ channel (K(Ca)) by 1-ethyl-2-benzimidazolinone or thapsigargin, methoxsalen (10 microM) further increased I(sc). 2) When added before carbachol (CCh), methoxsalen potentiated the I(sc) response to CCh, as predicted, if it increased apical Cl- conductance. 3) After establishment of a mucosal-to-serosal Cl- gradient and permeabilization of basolateral membrane with nystatin, psoralens increased Cl- current, which was inhibited by glibenclamide. In contrast, neither TS-TM calix[4]arene nor Cd2+, inhibitors of outwardly rectifying Cl- channels and the ClC-2 Cl-channel, respectively, inhibited psoralen-induced Cl- current. In contrast to their effects on Cl- conductance, psoralens failed to significantly affect basolateral membrane K+ conductance; subsequent addition of 1-ethyl-2-benzimidazolinone induced a large increase in K+ conductance. Also, in excised patches, methoxsalen failed to activate K(Ca). In addition to potentiating the peak response to CCh, psoralens induced a secondary, sustained response. Indeed, when added up to 60 min after return of CCh-induced I(sc) to baseline, psoralens induced a sustained I(sc). This sustained response was inhibited by atropine, demonstrating the requirement for continuous muscarinic receptor activation by CCh. This sustained response was inhibited also by verapamil, removal of bath Ca2+, and charybdotoxin. These results suggest that return of I(sc) to baseline after CCh stimulation is not due to downregulation of Ca2+ influx or K(Ca). Finally, we obtained similar results with psoralens in rat colon and primary cultures of murine tracheal epithelium. On the basis of these observations, we conclude that psoralens represent a novel class of Cl- channel openers that can be used to probe mechanisms underlying Ca2+-mediated Cl- secretion.

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Wang, Yi, Chengtao Hong, Chenguang Zhou, Dongmei Xu et Hai-bin Qu. « Screening Antitumor Compounds Psoralen and Isopsoralen fromPsoralea corylifoliaL. Seeds ». Evidence-Based Complementary and Alternative Medicine 2011 (2011) : 1–7. http://dx.doi.org/10.1093/ecam/nen087.

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Psoralea corylifoliaL. (Fabaceae) is a widely used medical plant in China. This study was designed to screen and identify bioactive compounds with anticancer activity from the seeds ofPsoralea corylifoliaL. One volatile fraction (fraction I) and three other fractions (fraction II, III, IV) from methanol extraction ofP. corylifoliaL. were obtained. Bioactivities of these fractions were evaluated by the cytotoxicity on KB, KBv200, K562, K562/ADM cancer cells with MTT assay. Major components in the active fraction were identified by HPLC/MSn. Fraction IV significantly inhibits the growth of cancer cells in a dose-dependent manner. The IC50values were 21.6, 24.4, 10.0 and 26.9, respectively. Psoralen and isopsoralen, isolated from fraction IV, were subject to bioactive assay and presented a dose-dependent anticancer activity in four cancer cell lines (KB, KBv200, K562 and K562/ADM). The IC50values of psoralen were 88.1, 86.6, 24.4 and 62.6, which of isopsoralen were 61.9, 49.4, 49.6 and 72.0, respectively. Apoptosis of tumor cell significantly increased after treated with psoralen and isopsoralen. Induction of apoptotic activity was confirmed by flow cytometry after staining with Annexin V/PI. These results suggested psoralen and isopsoralen contribute to anticancer effect ofP. corylifoliaL.

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Thakur, Abhinay, Rohit Sharma, Vivek Sheel Jaswal, Eugenie Nepovimova, Ashun Chaudhary et Kamil Kuca. « Psoralen : A Biologically Important Coumarin with Emerging Applications ». Mini-Reviews in Medicinal Chemistry 20, n^o 18 (7 décembre 2020) : 1838–45. http://dx.doi.org/10.2174/1389557520666200429101053.

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Coumarin belongs to a class of lactones that are fundamentally comprised of a benzene ring fused to an α-pyrone ring; these lactones are known as benzopyrones. Similarly, coumarin has a conjugated electron-rich framework and good charge-transport properties. Plants produce coumarin as a chemical response to protect themselves from predation. Coumarins are used in different products, such as cosmetics, additives, perfumes, aroma enhancers in various tobaccos and some alcoholic drinks, and they play a relevant role in natural products and in organic and medicinal chemistry. In addition, as candidate drugs, many coumarin compounds have strong pharmacological activity, low toxicity, high bioavailability and better curative effects and have been used to treat various types of diseases. Various endeavors were made to create coumarin-based anticoagulant, antimicrobial, antioxidant, anticancer, antidiabetic, antineurodegenerative, analgesic and anti-inflammatory agents. A class of chemical compounds called furocoumarins has phototoxic properties and is naturally synthesized via the fusion of coumarin to a furan ring in different plant species. Psoralens belong to the furocoumarin class and occur naturally in various plants, e.g., lemons, limes, and parsnips. Angelicin is an isomer of psoralens, and most furocoumarins, e.g., xanthotoxin, bergapten, and nodekenetin, are derivatives of psoralens or angelicin. The present work demonstrated that psoralen molecules exhibit anti-tumoral activity against breast cancer and influence different intracellular signals to maintain the high survival of breast cancer cells. Psoralens perform different functions, e.g., antagonize metabolic pathways, protease enzymes, and cell cycle progression and even interfere in the crosslinking between receptors and growth factor mitogenic signaling.

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Dubey, Nidhi, Nitin Dubey, Rajendra Mehta et Ajay Kumar Saluja. « Determination of Psoralen and Plumbagin from Its Polyherbal Oil Formulations by an HPTLC Densitometric Method ». Journal of AOAC INTERNATIONAL 92, n^o 3 (1 mai 2009) : 779–84. http://dx.doi.org/10.1093/jaoac/92.3.779.

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Abstract Many polyherbal oil formulations in Indian and Chinese traditional systems of medicine used for control of skin diseases contain seeds of Psoralea corylifolia and roots of Plumbago zeylanica L. Psoralen and plumbagin are the reliable markers for Ps. corylifolia and Pl. zeylanica, respectively. However, no attempt is made to standardize the polyherbal oil formulations containing Ps. corylifolia and Pl. zeylanica in terms of their active ingredients or marker compounds. In this paper, a simple, rapid, and sensitive HPTLC method is described for the first time to identify and quantify psoralen and plumbagin from such polyherbal oil formulations. The methanolic extract of oil formulations was used for analysis of markers. Psoralen gives a sharp UV absorbance peak at 302 nm and plumbagin at 275 nm. Good resolution of psoralen (Rf = 0.37) and plumbagin (Rf = 0.77) was attained using tolueneethyl acetate (7.5 + 2.5, v/v) mobile phase. The method was validated in terms of calibration curve, limits of detection and quantification, precision, accuracy, and robustness following a standard protocol. Polyherbal oil formulations were analyzed with reasonable accuracy, and no matrix interference was observed. The method developed can be used for marker-based quality assurance of oil formulations containing Ps. corylifolia and Pl. zeylanica as one active ingredient.

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Zhou, Yi-Ting, Lin Zhu, Yunyun Yuan, Shuang Ling et Jin-Wen Xu. « Effects and Mechanisms of Five Psoralea Prenylflavonoids on Aging-Related Diseases ». Oxidative Medicine and Cellular Longevity 2020 (18 juin 2020) : 1–21. http://dx.doi.org/10.1155/2020/2128513.

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During the aging process, senescent cells gradually accumulate in the organs; they secrete proinflammatory cytokines and other factors, collectively known as the senescence-associated secretory phenotype (SASP). SASP secretions contribute to “inflammaging,” which is a state of chronic, systemic, sterility, low-grade inflammatory microenvironment and a key risk factor in the development of aging-related diseases. Fructus psoraleae is a traditional Chinese medical herb best known for delaying aging and treating osteoporosis. Prenylflavonoids from fructus psoraleae are the main bioactive compounds responsible for its pharmacological applications, such as beaching, bavachinin, bavachalcone, isobavachalcone, and neobavaisoflavone. In previous decades, there have been some promising studies on the pharmacology of fructus psoraleae. Here, we focus on the anti-inflammatory and antiaging diseases of five psoralea prenylflavonoids, such as cardiovascular protection, diabetes and obesity intervention, neuroprotection, and osteoporosis, and discuss the mechanism of these active ingredients for better understanding the material basis and drug application of fructus psoraleae in Chinese medicine.

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Jin, Hualiang, Limin Wang, Changqing Xu, Bei Li, Qingli Luo, Jinfeng Wu, Yubao Lv, Genfa Wang et Jingcheng Dong. « Effects of Psoraleae fructus and Its Major Component Psoralen on Th2 Response in Allergic Asthma ». American Journal of Chinese Medicine 42, n^o 03 (janvier 2014) : 665–78. http://dx.doi.org/10.1142/s0192415x14500438.

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This study is aimed to evaluate the effects of Psoraleae fructus (PF) on Th2 responses in a rat model of asthma in vivo and psoralen, a major constituent in PF, on Th2 responses in vitro. A rat model of asthma was established by sensitization and challenged with ovalbumin (OVA). Airway hyperresponsiveness was detected by direct airway resistance analysis. Lung tissues were examined for cell infiltration and mucus hypersecretion. Bronchoalveolar lavage fluid (BALF) was assessed for cytokine levels. In vitro study, Th2 cytokine production was evaluated in the culture supernatant of D10.G4.1 (D10 cells) followed by the determination of cell viability, meanwhile Th2 transcription factor GATA-3 expression in D10 cells was also determined. The oral administration of PF significantly reduced airway hyperresponsiveness (AHR) to aerosolized methacholine and decreased IL-4 and IL-13 levels in the BALF. Histological studies showed that PF markedly inhibited inflammatory infiltration and mucus secretion in the lung tissues. In vitro study, psoralen significantly suppressed Th2 cytokines of IL-4, IL-5 and IL-13 by ConA-stimulated D10 cells without inhibitory effect on cell viability. Furthermore, GATA-3 protein expression was also markedly reduced by psoralen. This study demonstrated that PF exhibited inhibitory effects on hyperresponsiveness and airway inflammation in a rat model of asthma, which was associated with the suppression of Th2 response. Psoralen, a major constituent of PF, has immunomodulatory properties on Th2 response in vitro, which indicated that psoralen might be a critical component of PF for its therapeutic effects.

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Perera, Anthonige Vidya, James Brian Mendenhall, Charmain Tan Courcelle et Justin Courcelle. « Cho Endonuclease Functions during DNA Interstrand Cross-Link Repair in Escherichia coli ». Journal of Bacteriology 198, n^o 22 (29 août 2016) : 3099–108. http://dx.doi.org/10.1128/jb.00509-16.

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ABSTRACTDNA interstrand cross-links are complex lesions that covalently link both strands of the duplex DNA. Lesion removal is proposed to be initiated via the UvrABC nucleotide excision repair complex; however, less is known about the subsequent steps of this complex repair pathway. In this study, we characterized the contribution of nucleotide excision repair mutants to survival in the presence of psoralen-induced damage. Unexpectedly, we observed that the nucleotide excision repair mutants exhibit differential sensitivity to psoralen-induced damage, withuvrCmutants being less sensitive than eitheruvrAoruvrB. We show that Cho, an alternative endonuclease, acts with UvrAB and is responsible for the reduced hypersensitivity ofuvrCmutants. We find that Cho's contribution to survival correlates with the presence of DNA interstrand cross-links, rather than monoadducts, and operates at a step after, or independently from, the initial incision during the global repair of psoralen DNA adducts from the genome.IMPORTANCEDNA interstrand cross-links are complex lesions that covalently bind to both strands of the duplex DNA and whose mechanism of repair remains poorly understood. In this study, we show that Cho, an alternative endonuclease, acts with UvrAB and participates in the repair of DNA interstrand cross-links formed in the presence of photoactivated psoralens. Cho's contribution to survival correlates with the presence of DNA interstrand cross-links and operates at a step after, or independently from, the initial incision during the repair process.

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Chai, Lijuan, Kun Zhou, Shaoxia Wang, Han Zhang, Na Fan, Jie Li, Xiaofeng Tan, Limin Hu et Xiang Fan. « Psoralen and Bakuchiol Ameliorate M-CSF Plus RANKL-Induced Osteoclast Differentiation and Bone Resorption Via Inhibition of AKT and AP-1 Pathways in Vitro ». Cellular Physiology and Biochemistry 48, n^o 5 (2018) : 2123–33. http://dx.doi.org/10.1159/000492554.

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Background/Aims: Psoralen and bakuchiol are the main active compounds found in the traditional Chinese medicine Psoralea corylifolia L., and have been used to treat osteoporosis. This study aims to investigate the anti-osteoporosis effects of these two compounds using osteoclasts precursor differentiation and bone absorption assays in vitro. Methods: Primary mouse osteoclasts precursor cells were induced by M-CSF (macrophage colony stimulating factor) plus RANKL (receptor activator of nuclear factor kappa-B ligand) in vitro. TRACP (tartrate-resistant acid phosphatase) enzyme activity and toluidine blue staining were used to observe the effects of psoralen and bakuchiol on osteoclast differentiation and bone resorption, respectively. Gelatin zymography was used to assess MMP (matrix metalloproteinase) activity, and ELISA was performed to measure cathepsin K activity. Western blotting analysis for expression of phosphorylated AKT, ERK, NF-kB, and c-jun; and immunofluorescence analysis for c-jun and p65 nuclear translocation in induced osteoclasts were then used to determine the mechanism of anti-bone resorption of psoralen and bakuchiol. Results: Mature osteoclasts were induced by M-CSF plus RANKL from primary bone marrow macrophages in vitro. Both psoralen and bakuchiol significantly inhibited TRACP enzyme activity and slightly decreased the number of TRACP+ multinuclear osteoclasts induced by M-CSF plus RANKL. Bakuchiol significantly decreased bone lacunae area and attenuated MMP-2 activity induced by M-CSF plus RANKL in osteoclasts. Both psoralen and bakuchiol significantly decreased the expression and nuclear translocation of phosphorylated c-jun stimulated by M-CSF plus RANKL, but no significant effect on p65 translocation was observed in osteoclasts. Additionally, bakuchiol significantly attenuated the increased of M-CSF plus RANKL-induced phosphorylation of AKT in osteoclasts. Conclusions: Psoralen and bakuchiol ameliorated M-CSF plus RANKL-induced osteoclast differentiation and bone resorption via inhibition of AKT and AP-1 pathways activation in vitro.

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Machado, Rúbia Darc, Júlio C. G. Silva, Luís A. D. Silva, Gerlon de A. R. Oliveira, Luciano M. Lião, Eliana M. Lima, Mariana C. de Morais, Edemilson C. da Conceição et Kênnia R. Rezende. « Improvement in Solubility–Permeability Interplay of Psoralens from Brosimum gaudichaudii Plant Extract upon Complexation with Hydroxypropyl-β-cyclodextrin ». Molecules 27, n^o 14 (19 juillet 2022) : 4580. http://dx.doi.org/10.3390/molecules27144580.

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Psoralen (PSO) and 5-methoxypsoralen (5-MOP) are widely used drugs in oral photochemotherapy against vitiligo and major bioactive components of root bark extract of Brosimum gaudichaudii Trécul (EBGT), previously standardized by LC-MS. However, the exceptionally low water solubility of these psoralens can cause incomplete and variable bioavailability limiting their applications and patient adherence to treatment. Therefore, the purpose of this work was to investigate the effects of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex on the solubility and jejunal permeability of PSO and 5-MOP from EBGT. Characterization of inclusion complexes were evaluated by current methods in nuclear magnetic resonance studies on aqueous solution, Fourier transform infrared spectroscopy, thermal analysis, and scanning electron microscopy in solid state. Ex vivo rat jejunal permeability was also investigated and compared for both pure psoralens and plant extract formulation over a wide HP-β-CD concentration range (2.5 to 70 mM). Phase solubility studies of the PSO- and 5-MOP-HP-β-CD inclusion complex showed 1:1 inclusion complex formation with small stability constants (Kc < 500 M−1). PSO and 5-MOP permeability rate decreased after adding HP-β-CD by 6- and 4-fold for pure standards and EBGT markers, respectively. Nevertheless, the complexation with HP-β-CD significantly improved solubility of PSO (until 10-fold) and 5-MOP (until 31-fold). As a result, the permeability drop could be overcome by solubility augmentation, implying that the HP-β-CD inclusion complexes with PSO, 5-MOP, or EBGT can be a valuable tool for designing and developing novel oral drug product formulation containing these psoralens for the treatment of vitiligo.

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Xu, Yanyan, Yiwei Zhao, Jiabin Xie, Xue Sheng, Yubo Li et Yanjun Zhang. « The Evaluation of Toxicity Induced by Psoraleae Fructus in Rats Using Untargeted Metabonomic Method Based on UPLC-Q-TOF/MS ». Evidence-Based Complementary and Alternative Medicine 2017 (2017) : 1–9. http://dx.doi.org/10.1155/2017/6207183.

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Psoraleae Fructus is the dry and mature fruit of leguminous plant Psoralea corylifolia L., with the activity of warming kidney and enhancing yang, warming spleen, and other effects. However, large doses can cause liver and kidney toxicity. Therefore, it is necessary to evaluate the toxicity of Psoraleae Fructus systematically. Although traditional biochemical indicators and pathological tests have been used to evaluate the safety of drug, these methods lack sensitivity and specificity, so a fast and sensitive analytical method is urgently needed. In this study, an ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) method was used to analyze the metabolic profiles of rat plasma. The changes of metabolites in plasma samples were detected by partial least squares-discriminant analysis (PLS-DA). Compared with the control group, after 7 days of administration, the pathological sections showed liver and kidney toxicity, and the metabolic trend was changed. Finally, 13 potential biomarkers related to the toxicity of Psoraleae Fructus were screened. The metabolic pathways involved were glycerol phospholipids metabolism, amino acid metabolism, energy metabolism, and so forth. The discovery of these biomarkers laid a foundation for better explaining the hepatotoxicity and nephrotoxicity of Psoraleae Fructus and provided a guarantee for its safety evaluation.

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SUBHAN, SHAHNAZ, et M. Z. ABDIN M.Z. ABDIN. « Novel and Simple Method for in Vitro Production of Psoralen via Cotyledonary Culture of An Endangered Leguminous Taxon- Psoralea Corylifolia Linn. » Indian Journal of Applied Research 4, n^o 4 (1 octobre 2011) : 500–503. http://dx.doi.org/10.15373/2249555x/apr2014/158.

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Martins, Frederico Severino, Sherwin K. B. Sy, Maria José Vieira Fonseca et Osvaldo de Freitas. « Pharmacokinetics, Pharmacodynamics and Dermal Distribution of 5-Methoxypsoralen Based on a Physiologically Based Pharmacokinetic Model to Support Phytotherapy Using Brosimum gaudichaudii ». Planta Medica 86, n^o 04 (22 janvier 2020) : 276–83. http://dx.doi.org/10.1055/a-1087-8374.

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AbstractThe treatment of vitiligo includes the combination of psoralens and ultraviolet type A exposure. Psoralens belong to a group of natural furanocoumarins that cause the skin to become sensitive temporarily to ultraviolet type A. The aim of this study was to develop a physiologically based pharmacokinetic model of 5-MOP from Brosimum gaudichaudii to support psoralen and ultraviolet type A therapy. A study of rats was used to establish and validate rat tissue distribution. The same chemical-specific parameters used in the rat model were also employed in the human model to project human pharmacokinetics. The highest exposures in the rats were in the brain and skin. Following a single dose of 1.2 mg/kg 5-MOP in humans, the model predicted a maximum concentration of 20 ng/mL and an area under the curve of 125 ng.h/mL, matching clinical results. The half-maximum melanogenesis concentrations in B16F10 cells were 29.5, 18.5, 11.5, and 6.5 ng/mL for synthetic 5-MOP, synthetic 5-MOP with ultraviolet type A, B. gaudichaudii alone, and B. gaudichaudii plus ultraviolet type A, respectively. Physiologically based pharmacokinetic model prediction in humans supported a once-every-two-day regimen for optimal melanin production. This type of framework can be applied to support strategies for dose selection and to investigate the impact of drugs on melanocyte recovery.

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Hamerski, Daria, et Ulrich Matern. « Biosynthesis of psoralens Psoralen 5-monooxygenase activity from elicitor-treated Ammi majus cells ». FEBS Letters 239, n^o 2 (7 novembre 1988) : 263–65. http://dx.doi.org/10.1016/0014-5793(88)80930-x.

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Ariantari, Ni Putu, et Elizabeth S. P. Ratnasantasyacitta. « Pharmacologically Active Secondary Metabolites from Psoralea corylifolia ». Journal of Tropical Pharmacy and Chemistry 6, n^o 2 (28 novembre 2022) : 177–89. http://dx.doi.org/10.25026/jtpc.v6i2.431.

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Psoralea corylifolia has gained much attention, particularly in the cosmetic industry for the past few years owing to promising pharmacological activities of its metabolites. Seeds of P. corylifolia are the main source of bakuchiol, a meroterpene compound that is extensively harnessed in numerous skincare products. Furanocoumarins, psoralen and isopsoralen are other metabolites mainly from P. corylifolia seeds and known for their antipsoriatic activity. Moreover, various studies have reported several classes of secondary metabolites from this plant possessing diverse biological activities. This article highlights recent updates on P. corylifolia phytoconstituents and their promising pharmacological activities based on scientific publications during the last 10 years (2011-2021). The literature search was carried out through scientific-based websites and databases such as Google Scholar, NCBI, and PubMed. This paper included sixty-three bioactive metabolites reported in the last 10 years, belonging to the group of flavonoids, meroterpenes, furanocoumarins, coumestans, steroid and phenolic compounds. These phytoconstituents displayed a broad range of bioactivities including anti-inflammatory, antibacterial, antidiabetic, controlling obesity, hepatoprotective and cytotoxicity. Keywords: Bakuchiol, pharmacological activities, Psoralea corylifolia, bioactive metabolites.

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Hou, Jingxia, Shangyang Lin, Jinlan Lu, Yu Wu, Li Wu, Zhipeng Chen et Weidong Li. « Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats ». Journal of Analytical Methods in Chemistry 2020 (17 septembre 2020) : 1–15. http://dx.doi.org/10.1155/2020/8832736.

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Qing’e pills is clinically used for treating osteoporosis in postmenopausal women in China. Eucommiae Cortex and Psoraleae Fructus are the main herbs of Qing’e pills and are both required to be salt-processed. In order to study the influence of salt-processing on the tissue distribution of Qing’e pills, a UPLC-MS/MS method was established for studying the tissue distribution of 12 main bioactive ingredients of Qing’e pills in rats. The linear relationships of the 12 compounds in each tissue were good. The method was fully validated for its selectivity, accuracy, precision, stability, matrix effect, and extraction recovery. Then, the validated method was successfully applied for simultaneous determination of the 12 chemical components in Qing’e pills in tissues for the first time. Areas under the curve (AUC) results showed that, except for pinoresinol diglucoside, psoralen, and isopsoralen, the distribution of the other components was increased in the kidney, uterus, ovary, and testes. Relative targeting efficiency (RTE) results showed that all 12 chemical components targeted the kidney and sexual organs. The results indicated that the Eucommiae Cortex and Psoraleae Fructus after salt-processing could significantly increase the distribution of components to the kidney and generative organs.

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Ali, J., N. Akhtar, Y. Sultana, S. Baboota et S. Ahmad. « Thin-layer chromatographic analysis of psoralen in babchi (Psoralea corylifolia) oil ». Acta Chromatographica 20, n^o 2 (juin 2008) : 277–82. http://dx.doi.org/10.1556/achrom.20.2008.2.12.

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Parast, Behrooz M., Siva K. Chetri, Kuldeep Sharma et Veena Agrawal. « In vitro isolation, elicitation of psoralen in callus cultures of Psoralea corylifolia and cloning of psoralen synthase gene ». Plant Physiology and Biochemistry 49, n^o 10 (octobre 2011) : 1138–46. http://dx.doi.org/10.1016/j.plaphy.2011.03.017.

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Baskaran, P., et N. Jayabalan. « Psoralen production in hairy roots and adventitious roots cultures of Psoralea coryfolia ». Biotechnology Letters 31, n^o 7 (7 mars 2009) : 1073–77. http://dx.doi.org/10.1007/s10529-009-9957-9.

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Zobel, Alicja M., et Stewart A. Brown. « Psoralens on the surface of seeds of Rutaceae and fruits of Umbelliferae and Leguminosae ». Canadian Journal of Botany 69, n^o 3 (1 mars 1991) : 485–88. http://dx.doi.org/10.1139/b91-065.

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Linear furanocoumarins (psoralens) were present both inside and on the surface of dry fruits of seven species of Umbelliferae and one species of the Leguminosae and on seeds of three of Rutaceae examined. Surface concentrations varied between traces and 40 μg/g of the mature fruits or seeds, amounting to 0.38–43% of the total. In the whole fruits concentrations were very high in Angelica archangelica, Heracleum lanatum, Pastinaca saliva, and Psoralea bituminosa, reaching levels of milligrams per gram fresh weight of fruits. This high concentration inside the fruit may be the cause of poor germination of embryos in A. archangelica and H. lanatum. Seeds of the Rutaceae had much smaller concentrations of furanocoumarins both on the surface and within the seed. Key words: fruits, seeds, surface, furanocoumarins, Umbelliferae, Rutaceae, dermatitis, plant defense.

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Ahmed, Syed Abrar, et Mirza Mushtaq Vaseem Baig. « Biotic elicitor enhanced production of psoralen in suspension cultures of Psoralea corylifolia L. » Saudi Journal of Biological Sciences 21, n^o 5 (novembre 2014) : 499–504. http://dx.doi.org/10.1016/j.sjbs.2013.12.008.

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Mahajan, Neha, Bhupendra Koul, Jasleen Kaur, Mahendra Bishnoi, Pankaj Gupta, Amit Kumar, Bhahwal Ali Shah et al. « Antiobesity Potential of Bioactive Constituents from Dichloromethane Extract of Psoralea corylifolia L. Seeds ». BioMed Research International 2022 (25 août 2022) : 1–10. http://dx.doi.org/10.1155/2022/9504787.

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Purpose. Effectively controlling the accumulation of adipose tissue can be a therapeutic strategy for treating obesity, which is a global problem. The present study was designed for comparative assessment of in vitro antiobesity activities of the Psoralea corylifolia-dichloromethane seed extract (DCME) and the isolated phytochemicals, bakuchiol, isopsoralen, and psoralen, through antiadipogenesis and pancreatic lipase (PL) inhibition assays. Material and Methods. In vitro pancreatic lipase activity was determined spectrophotometrically by measuring the hydrolysis of p-nitrophenyl butyrate (p-NPB) to p-nitrophenol at 405 nm, and adipogenesis was assayed in 3 T3-L1 adipocytes (by using Oil Red O staining) using P. corylifolia-dichloromethane seed extract (DCME) and individual compounds, isolated from the extract. Result. Antilipase as well as antiadipogenesis activity was displayed by both the DCME and the compounds. Maximum antilipase property was recorded in DCME ( 26.02 ± .041 %) at 100 μg/ml, while, among the isolated compounds, bakuchiol exhibited a higher activity ( 24.2 ± 0.037 %) at 100 μg/ml concentration, compared to other isolates. DCME was found to exhibit antiadipogenesis property, 75 ± 0.003 % lipid accumulation, compared to the control at 100 μg/ml dose. Bakuchiol, isopsoralen, and psoralen inhibited the lipid accumulation in 3T3-L1 preadipocytes, 78.06 ± 0.002 %, 80.91 ± 0.004 %, and 80.91 ± 0.001 %, respectively, lipid accumulation in comparison to control at 25 μM dose. Conclusion. The present study highlights the antiobesity potential of P. corylifolia and its active constituents. Thus, it can be concluded that P. corylifolia has the potential to treat obesity and related diseases; however, further research on dose standardization and clinical trials are required.

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Agrawal, V., S. P. K. Chetri et B. M. Parast. « IN VITRO EVALUATION OF PSORALEN THROUGH NODAL CULTURES OF PSORALEA CORYLIFOLIA EXPOSED TO ELICITORS ». Acta Horticulturae, n^o 972 (janvier 2013) : 9–20. http://dx.doi.org/10.17660/actahortic.2013.972.1.

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Baskaran, P., et N. Jayabalan. « Effect of growth regulators on rapid micropropagation and psoralen production in Psoralea corylifolia L. » Acta Physiologiae Plantarum 30, n^o 3 (9 janvier 2008) : 345–51. http://dx.doi.org/10.1007/s11738-007-0129-z.

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Aruna, V., GV Amruthavalli et R. Gayathri. « Safety profile of synthetic versus natural Psoralen ». Journal of Medical Research 5, n^o 5 (25 octobre 2019) : 194–97. http://dx.doi.org/10.31254/jmr.2019.5507.

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Psoralen is used widely in the treatment of various skin diseases such as Psoriasis, Vitiligo etc. The Psoralen used for the above purpose is in oral dosage form and the topical dosage form is likely to reduce the severe side effects of oral Psoralen. However the phototoxic effect of Psoralen in topical form limit the medical fraternity from accepting it when compared to oral Psoralen. The safety profile of synthetic Psoralen versus natural Psoralen was done and natural Psoralen was found to be non-genotoxic and exerted least toxicity to keratinocytes and melanocytes when the cells were exposerd to UV exposure. The present study clearly conclude that natural Psoralen is extremely safe over synthetic Psoralen and the topical dosage form can bring down the side effects and enhance the treatment success. Details are discussed in the paper.

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Wu, Chi-Rei, Chia-Lin Chang, Ping-Ying Hsieh, Li-Wei Lin et Hui Ching. « Psoralen and Isopsoralen, Two Coumarins of Psoraleae Fructus, can Alleviate Scopolamine-Induced Amnesia in Rats ». Planta Medica 73, n^o 3 (mars 2007) : 275–78. http://dx.doi.org/10.1055/s-2007-967127.

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Wang, Yuhao, Yonglun Wang, Fang Li, Jie Zou, Xiaoqian Li, Mengxia Xu, Daojiang Yu et al. « Psoralen Suppresses Lipid Deposition by Alleviating Insulin Resistance and Promoting Autophagy in Oleate-Induced L02 Cells ». Cells 11, n^o 7 (22 mars 2022) : 1067. http://dx.doi.org/10.3390/cells11071067.

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Non-alcoholic fatty liver disease (NAFLD) held a high global prevalence in recent decades. Hepatic lipid deposition is the major characteristic of NAFLD. We aim to explore the mechanisms of psoralen on lipid deposition in NAFLD. The effects of psoralen on insulin resistance, lipid deposition, the expression and membrane translocation of glucose transporter type 4 (GLUT4), autophagy, and lipogenesis enzymes were determined on sodium oleate-induced L02 cells. Chloroquine and 3-MA were employed. The AMP-activated protein kinase alpha (AMPKα) was knocked down by siRNA. Psoralen alleviated insulin resistance in sodium oleate-induced L02 hepatocytes by upregulating the expression and membrane translocation of GLUT4. Psoralen inhibited lipid accumulation by decreasing the expression of key lipogenesis enzymes. Psoralen promotes autophagy and the autophagic flux to enhance lipolysis. Psoralen promoted the fusion of the autophagosome with the lysosome. Both chloroquine and 3-MA blocked the effects of psoralen on autophagy and lipid accumulation. The AMPKα deficiency attenuated the effects of psoralen on autophagy and lipid accumulation. Our study demonstrated that as an antioxidant, psoralen attenuates NAFLD by alleviating insulin resistance and promoting autophagy via AMPK, suggesting psoralen to be a promising candidate for NAFLD.

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Sultan, Tahira, et Sharique A. Ali. « Psoralea corylifoliaextracts stimulate cholinergic-like psoralen receptors of tadpole-tail melanophores, leading to skin darkening ». Journal of Receptors and Signal Transduction 31, n^o 1 (23 septembre 2010) : 39–44. http://dx.doi.org/10.3109/10799893.2010.508164.

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Baskaran, P., N. Jayabalan et J. Van Staden. « Production of psoralen by in vitro regenerated plants from callus cultures of Psoralea corylifolia L. » Plant Growth Regulation 65, n^o 1 (23 février 2011) : 47–54. http://dx.doi.org/10.1007/s10725-011-9573-2.

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Hari, Gajula, Kumar Vadlapudi, Poornima D. Vijendra, Rajashekar J., Torankumar Sannabommaji et Giridhara Basappa. « A combination of elicitor and precursor enhances psoralen production in Psoralea corylifolia Linn. suspension cultures ». Industrial Crops and Products 124 (novembre 2018) : 685–91. http://dx.doi.org/10.1016/j.indcrop.2018.08.050.

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Afek, U., N. Aharoni et S. Carmeli. « Increasing Celery Resistance to Pathogens during Storage and Reducing High-risk Psoralen Concentration by Treatment with GA3 ». Journal of the American Society for Horticultural Science 120, n^o 4 (juillet 1995) : 562–65. http://dx.doi.org/10.21273/jashs.120.4.562.

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Since psoralens have a very weak antifungal activity in vitro, we propose that (+)marmesin, the precursor of psoralens in celery (Apium graveolens L.) is associated with celery resistance to pathogens. (+)Marmesin has at least 100 times greater antifungal activity in vitro than psoralens. After 1 month of storage at 2C, the concentration of total psoralens increased from 8 to 67 μg·g-1 fresh weight, (+)marmesin decreased from 27 to 4 μg·g-1 fresh weight, and the incidence of decay increased from 0% to 34%. However, when celery was treated with GA3before 1 month of storage at 2C, decay increased to only 7%, the concentration of psoralens increased to 31 μg·g-1 fresh weight and the concentration of (+)marmesin decreased to 13 μg·g-1 fresh weight It seems that GA3 retarded celery decay during storage by slowing down the conversion of (+)marmesin to psoralens, thereby increasing the resistance to pathogens during storage.

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Wu, Yang, Yong-Zheng Zhang, Meng-Jia Li, Wen-qing Yang et Lu-feng Cheng. « The In Vitro Effect of Psoralen on Glioma Based on Network Pharmacology and Potential Target Research ». Evidence-Based Complementary and Alternative Medicine 2022 (27 août 2022) : 1–10. http://dx.doi.org/10.1155/2022/1952891.

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Glioma is an aggressive tumor, currently there is no satisfactory management available. Psoralen, as a natural product, has been found to have an effect of treating cancer in recent years, but its effect on glioma has not been explored. In this study, we investigated the in vitro inhibition effect and potential targets of psoralen on glioma through network pharmacology and in vitro glioma treatment experiments. First, we used network pharmacology to preliminarily predict the 21 core genes of psoralen in the treatment of glioma, including PIK3CA, PIK3CB, PIK3CG, and JAK2. The CCK-8 method was used to detect the effect of psoralen on the proliferation of glioma U87 and U251 cells, and the results showed that psoralen could significantly inhibit the proliferation of U87 and U251 cells. The flow cytometry was used to detect the apoptosis and cell cycle changes, and it was found that psoralen could significantly promote the early apoptosis of U87 and U251 cells and had a significant cycle arrest effect on the two cells. The cell scratch test showed that psoralen could significantly inhibit the migration of U87 and U251 cells. The relative expression levels of PIK3CA, PIK3CB, PIK3CG, and JAK2 were analyzed by Real-time Quantitative polymerase chain reaction (QT-PCR), and the results showed that psoralen could inhibit the gene expression of PIK3CA, PIK3CB, PIK3CG, and JAK2. Later, Western blotting (WB) experiments showed that psoralen could inhibit the protein expressions of PI3K and JAK2. This study has preliminarily explored and verified the antiglioma effect of psoralen in the form of inhibiting cell proliferation and migration, promoting cell apoptosis and organizing cell cycle in vitro. And may play a role by inhibiting the expression of PIK3CA, PIK3CB, PIK3CG, JAK2 gene and PI3K, JAK2 protein, psoralen has become a potential antiglioma drug.

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Lu, Jinlan, Ling Liu, Xingyu Zhu, Li Wu, Zhipeng Chen, Zisheng Xu et Weidong Li. « Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model ». Molecules 23, n^o 12 (14 décembre 2018) : 3321. http://dx.doi.org/10.3390/molecules23123321.

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Qing’e Pills is a Chinese traditional herbal product, which is often used to strengthen muscles and bones in TCM (traditional Chinese Medicine) practice. Its two main component herbs, namely, Cortex Eucommiae and Fructus Psoraleae are both required to be salt-fried according to TCM theory. We have evaluated the effects of salt-frying treated herbs on Caco-2 cell uptake behavior for those active ingredients of Qing’e Pills. By investigating of various variables, including MTT, temperature, inhibitors, pH, salt concentration and herb processing methods, we tried to clarify whether the salt-processing on herbs was necessary or not. Results showed that, compared to other processing methods, the salt-frying process significantly (p < 0.01) enhanced the absorption of effective components of Qing’e Pills. The way that psoralen, isopsoralen, psoralenoside and geniposide acid entered Caco-2 cells at low concentrations was via passive diffusion. These components were not substrates of P-glycoprotein. It demonstrated that the salt-frying process not only enhanced the concentration of active components in herb extract, but also changed their absorption behaviors. Nevertheless, the mechanism of absorption behavior changing needs to be further investigated.

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&NA;. « Psoralens ». Reactions Weekly &NA;, n^o 1166 (août 2007) : 19–20. http://dx.doi.org/10.2165/00128415-200711660-00060.

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&NA;. « Psoralens ». Reactions Weekly &NA;, n^o 1123 (octobre 2006) : 18–19. http://dx.doi.org/10.2165/00128415-200611230-00052.

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&NA;. « Psoralens ». Reactions Weekly &NA;, n^o 1136 (janvier 2007) : 22. http://dx.doi.org/10.2165/00128415-200711360-00070.

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&NA;. « Psoralens ». Reactions Weekly &NA;, n^o 1156 (juin 2007) : 22–23. http://dx.doi.org/10.2165/00128415-200711560-00068.

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