Articles de revues : « Pseudomonas savastanoi »

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Pérez-Martínez, Isabel, Youfu Zhao, Jesús Murillo, George W. Sundin et Cayo Ramos. « Global Genomic Analysis of Pseudomonas savastanoi pv. savastanoi Plasmids ». Journal of Bacteriology 190, n^o 2 (9 novembre 2007) : 625–35. http://dx.doi.org/10.1128/jb.01067-07.

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ABSTRACT Pseudomonas savastanoi pv. savastanoi strains harbor native plasmids belonging to the pPT23A plasmid family (PFPs) which are detected in all pathovars of the related species Pseudomonas syringae examined and contribute to the ecological and pathogenic fitness of their host. However, there is a general lack of information about the gene content of P. savastanoi pv. savastanoi plasmids and their role in the interaction of this pathogen with olive plants. We designed a DNA macroarray containing 135 plasmid-borne P. syringae genes to conduct a global genetic analysis of 32 plasmids obtained from 10 P. savastanoi pv. savastanoi strains. Hybridization results revealed that the number of PFPs per strain varied from one to four. Additionally, most strains contained at least one plasmid (designated non-PFP) that did not hybridize to the repA gene of pPT23A. Only three PFPs contained genes involved in the biosynthesis of the virulence factor indole-3-acetic acid (iaaM, iaaH, and iaaL). In contrast, ptz, a gene involved in the biosynthesis of cytokinins, was found in five PFPs and one non-PFP. Genes encoding a type IV secretion system (T4SS), type IVA, were found in both PFPs and non-PFPs; however, type IVB genes were found only on PFPs. Nine plasmids encoded both T4SSs, whereas seven other plasmids carried none of these genes. Most PFPs and non-PFPs hybridized to at least one putative type III secretion system effector gene and to a variety of additional genes encoding known P. syringae virulence factors and one or more insertion sequence transposase genes. These results indicate that non-PFPs may contribute to the virulence and fitness of the P. savastanoi pv. savastanoi host. The overall gene content of P. savastanoi pv. savastanoi plasmids, with their repeated information, mosaic arrangement, and insertion sequences, suggests a possible role in adaptation to a changing environment.

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Matas, Isabel M., Isabel Pï¿½rez-Martï¿½nez, Josï¿½ M. Quesada, Josï¿½ J. Rodrï¿½guez-Herva, Ramï¿½n Penyalver et Cayo Ramos. « Pseudomonas savastanoi pv. savastanoi Contains Two iaaL Paralogs, One of Which Exhibits a Variable Number of a Trinucleotide (TAC) Tandem Repeat ». Applied and Environmental Microbiology 75, n^o 4 (19 décembre 2008) : 1030–35. http://dx.doi.org/10.1128/aem.01572-08.

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ABSTRACT In this study, Pseudomonas savastanoi pv. savastanoi isolates were demonstrated to contain two iaaL paralogs, which are both chromosomally located in most strains. Comparative analysis of iaaL nucleotide sequences amplified from these two paralogs revealed that one paralog, iaaL Psn, is 100% identical to iaaL from P. savastanoi pv. nerii, while the other paralog, iaaL Psv, exhibited 93% identity to iaaL from Pseudomonas syringae pv. tomato (iaaL Pto). A 3-nucleotide motif (TAC) comprised of 3 to 15 repeats, which remained stable after propagation of the strains in olive plants, was found in iaaL Psv. Based on the observed nucleotide sequence variations, a restriction fragment length polymorphism assay was developed that allowed differentiation among iaaL Psn, iaaL Psv, and iaaL Pto . In addition, reverse transcriptase PCR on total RNA from P. savastanoi pv. savastanoi strains demonstrated that both iaaL Psv and iaaL Psn containing 14 or fewer TAC repeats are transcribed. Capillary electrophoresis analysis of PCR-amplified DNA fragments containing the TAC repeats from iaaL Psv allowed the differentiation of P. savastanoi pv. savastanoi isolates.

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Rodríguez-Moreno, Luis, Antonio J. Jiménez et Cayo Ramos. « Endopathogenic lifestyle of Pseudomonas savastanoi pv. savastanoi in olive knots ». Microbial Biotechnology 2, n^o 4 (18 mars 2009) : 476–88. http://dx.doi.org/10.1111/j.1751-7915.2009.00101.x.

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Penyalver, Ramón, Amparo García, Amparo Ferrer, Edson Bertolini et María M. López. « Detection of Pseudomonas savastanoi pv. savastanoi in Olive Plants by Enrichment and PCR ». Applied and Environmental Microbiology 66, n^o 6 (1 juin 2000) : 2673–77. http://dx.doi.org/10.1128/aem.66.6.2673-2677.2000.

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ABSTRACT The sequence of the gene iaaL of Pseudomonas savastanoi EW2009 was used to design primers for PCR amplification. The iaaL-derived primers directed the amplification of a 454-bp fragment from genomic DNA isolated from 70 strains of P. savastanoi, whereas genomic DNA from 93 non-P. savastanoi isolates did not yield this amplified product. A previous bacterial enrichment in the semiselective liquid medium PVF-1 improved the PCR sensitivity level, allowing detection of 10 to 100 CFU/ml of plant extract. P. savastanoi was detected by the developed enrichment-PCR method in knots from different varieties of inoculated and naturally infected olive trees. Moreover,P. savastanoi was detected in symptomless stem tissues from naturally infected olive plants. This enrichment-PCR method is more sensitive and less cumbersome than the conventional isolation methods for detection of P. savastanoi.

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Pï¿½rez-Martï¿½nez, Isabel, Luis Rodrï¿½guez-Moreno, Lotte Lambertsen, Isabel M. Matas, Jesï¿½s Murillo, Stefania Tegli, Antonio J. Jimï¿½nez et Cayo Ramos. « Fate of a Pseudomonas savastanoi pv. savastanoi Type III Secretion System Mutant in Olive Plants (Olea europaea L.) ». Applied and Environmental Microbiology 76, n^o 11 (2 avril 2010) : 3611–19. http://dx.doi.org/10.1128/aem.00133-10.

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ABSTRACT Pseudomonas savastanoi pv. savastanoi strain NCPPB 3335 is a model bacterial pathogen for studying the molecular basis of disease production in woody hosts. We report the sequencing of the hrpS-to-hrpZ region of NCPPB 3335, which has allowed us to determine the phylogenetic position of this pathogen with respect to previously sequenced Pseudomonas syringae hrp clusters. In addition, we constructed a mutant of NCPPB 3335, termed T3, which carries a deletion from the 3′ end of the hrpS gene to the 5′ end of the hrpZ operon. Despite its inability to multiply in olive tissues and to induce tumor formation in woody olive plants, P. savastanoi pv. savastanoi T3 can induce knot formation on young micropropagated olive plants. However, the necrosis and formation of internal open cavities previously reported in knots induced by the wild-type strain were not observed in those induced by P. savastanoi pv. savastanoi T3. Tagging of P. savastanoi pv. savastanoi T3 with green fluorescent protein (GFP) allowed real-time monitoring of its behavior on olive plants. In olive plant tissues, the wild-type strain formed aggregates that colonized the intercellular spaces and internal cavities of the hypertrophic knots, while the mutant T3 strain showed a disorganized distribution within the parenchyma of the knot. Ultrastructural analysis of knot sections revealed the release of extensive outer membrane vesicles from the bacterial cell surface of the P. savastanoi pv. savastanoi T3 mutant, while the wild-type strain exhibited very few vesicles. This phenomenon has not been described before for any other bacterial phytopathogen during host infection.

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Moreno-Pérez, Alba, Cayo Ramos et Luis Rodríguez-Moreno. « HrpL Regulon of Bacterial Pathogen of Woody Host Pseudomonas savastanoi pv. savastanoi NCPPB 3335 ». Microorganisms 9, n^o 7 (5 juillet 2021) : 1447. http://dx.doi.org/10.3390/microorganisms9071447.

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The Pseudomonas savastanoi species comprises a group of phytopathogenic bacteria that cause symptoms of disease in woody hosts. This is mediated by the rapid activation of a pool of virulence factors that suppress host defences and hijack the host’s metabolism to the pathogen’s benefit. The hrpL gene encodes an essential transcriptional regulator of virulence functions, including the type III secretion system (T3SS), in pathogenic bacteria. Here, we analyzed the contribution of HrpL to the virulence of four pathovars (pv.) of P. savastanoi isolated from different woody hosts (oleander, ash, broom, and dipladenia) and characterized the HrpL regulon of P. savastanoi pv. savastanoi NCPPB 3335 using two approaches: whole transcriptome sequencing (RNA-seq) and the bioinformatic prediction of candidate genes containing an hrp-box. Pathogenicity tests carried out for the P. savastanoi pvs. showed that HrpL was essential for symptom development in both non-host and host plants. The RNA-seq analysis of the HrpL regulon in P. savastanoi revealed a total of 53 deregulated genes, 49 of which were downregulated in the ΔhrpL mutant. Bioinformatic prediction resulted in the identification of 50 putative genes containing an hrp-box, 16 of which were shared with genes previously identified by RNA-seq. Although most of the genes regulated by HrpL belonged to the T3SS, we also identified some genes regulated by HrpL that could encode potential virulence factors in P. savastanoi.

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Martin, J. L., S. D. Comfort, P. J. Shea, R. A. Drijber et T. A. Kokjohn. « Denitration of 2,4,6-trinitrotoluene byPseudomonas savastanoi ». Canadian Journal of Microbiology 43, n^o 5 (1 mai 1997) : 447–55. http://dx.doi.org/10.1139/m97-063.

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Past disposal of wastewaters containing 2,4,6-trinitrotoluene (TNT) at the former Nebraska Ordnance Plant has resulted in numerous acres of TNT-contaminated soil. Examining the microbial population of these soils revealed several TNT-tolerant Pseudomonas spp. We selected one species, P. savastanoi, to determine its ability to transform TNT. Pure culture experiments were performed in pseudomonas minimal medium containing 0.31 mM TNT (70 mg TNT∙L−1) under varied nutrient and cell density regimes. Experiments with TNT as a sole C or N source showed that P. savastanoi has the ability to denitrate TNT, as evidenced by production of 2,4-dinitrotoluene (2,4-DNT) and NO2−with time. TNT denitration and formation of 2,4-DNT were enhanced by removing NH4+and adding NO2−to the growth medium. In all experiments, 2-amino-4,6-dinitrotoluene (2-ADNT) and 4-amino-2,6-dinitrotoluene (4-ADNT) appeared as incidental reduction products. Glucose addition to the medium enhanced 2-ADNT and 4-ADNT production and decreased denitration of TNT. Mid-log phase cells rapidly transformed [ring-14C(U)]TNT but were unable to mineralize significant quantities of TNT, as evidenced by conversion of less than 1% of the label to14CO2. These results indicate that P. savastanoi is a TNT-tolerant pseudomonad that can promote TNT degradation through reductive denitration and nitro moiety reduction.Key words: TNT, biodegradation, transformation, reduction, nitrite.

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Rodríguez-Moreno, Luis, Araceli Barceló-Muñoz et Cayo Ramos. « In Vitro Analysis of the Interaction of Pseudomonas savastanoi pvs. savastanoi and nerii with Micropropagated Olive Plants ». Phytopathology® 98, n^o 7 (juillet 2008) : 815–22. http://dx.doi.org/10.1094/phyto-98-7-0815.

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This study assessed the use of in vitro olive plants to evaluate the virulence of Pseudomonas savastanoi pv. savastanoi strains isolated from olive and P. savastanoi pv. nerii strains isolated from oleander knots. First, different olive isolates were inoculated into stem wounds and differences in knot formation and weight of overgrowths were observed for the selected strains. Tissue proliferation was clearly visible in all inoculated plants 30 days after inoculation. Virulence of P. savastanoi pv. nerii mutants with defects in regard to biosynthesis of indole-3-acetic acid and/or cytokinins was tested using this system. In agreement with data previously reported, all mutant strains multiplied in olive but induced attenuated symptoms. To analyze the virulence of P. savastanoi pv. savastanoi affected in their ability to grow in olive tissue, a trpE tryptophan auxotroph mutant was generated using a collection of signature tagged mutagenesis transposons. Virulence of this mutant was clearly reduced as evidenced by swelling of the olive tissue that evolved into attenuated knots. Furthermore, mixed infections with its parental strain revealed that the wild-type strain completely out-competed the trpE mutant. Results shown here demonstrate the usefulness of in vitro olive plants for the analysis of P. savastanoi pvs. savastanoi and nerii virulence. In addition, this system offers the possibility of quantifying virulence differences as weight of overgrowths. Moreover, we established the basis for the use of mixed infections in combination with signature tagged mutagenesis for high-throughput functional genomic analysis of this bacterial pathogen.

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Marchi, G., A. Sisto, A. Cimmino, A. Andolfi, M. G. Cipriani, A. Evidente et G. Surico. « Interaction between Pseudomonas savastanoi pv. savastanoi and Pantoea agglomerans in olive knots ». Plant Pathology 55, n^o 5 (octobre 2006) : 614–24. http://dx.doi.org/10.1111/j.1365-3059.2006.01449.x.

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Quesada, Jose M., Isabel Pérez-Martínez, Cayo Ramos, María M. López et Ramón Penyalver. « IS53 : an insertion element for molecular typing of Pseudomonas savastanoi pv. savastanoi ». Research in Microbiology 159, n^o 3 (avril 2008) : 207–15. http://dx.doi.org/10.1016/j.resmic.2007.12.010.

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Sisto, A., M. G. Cipriani et M. Morea. « Knot Formation Caused by Pseudomonas syringae subsp. savastanoi on Olive Plants Is hrp-Dependent ». Phytopathology® 94, n^o 5 (mai 2004) : 484–89. http://dx.doi.org/10.1094/phyto.2004.94.5.484.

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The virulence of Pseudomonas syringae subsp. savastanoi, which causes hyperplastic symptoms (knots) on olive plants, is associated with secreted phytohormones. We identified a Tn5-induced mutant of P. syringae subsp. savastanoi that did not cause disease symptoms on olive plants although it was still able to produce phytohormones. In addition, the mutant failed to elicit a hypersensitive response in a nonhost plant. Molecular characterization of the mutant revealed that a single Tn5 insertion occurred within an open reading frame encoding a protein 92% identical to the HrcC protein of P. syringae pv. syringae. Moreover, sequence analysis revealed that the gene encoding the HrcC protein in P. syringae subsp. savastanoi was part of an operon that included five genes arranged as in other phytopathogenic bacteria. These results imply that hrp/hrc genes are functional in P. syringae subsp. savastanoi and that they play a key role in the pathogenicity of this plant pathogen.

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Kůdela, V., I. Šafránková, V. Krejzar et J. Korba. « First report of Pseudomonas savastanoi pv. nerii on oleander in the Czech Republic ». Plant Protection Science 41, No. 1 (8 février 2010) : 33–37. http://dx.doi.org/10.17221/2733-pps.

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The bacterium Pseudomonas savastanoi pv. nerii was identified as the causal agent of parenchymatous galls on leaves of potted oleander plants grown at Brno, Moravia, in 2004. The plants had originated from cuttings made from firm shoots of a supposedly asymptomatic plant grown in and introduced from the Mediterranean region. The Biolog GN microplate system was used to identify the isolated bacterial strains. Successful inoculation of Nerium oleander seedlings proved the pathogenicity of the isolates. This is the first record of P. savastanoi pv. nerii in the Czech Republic. :

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Caballo-Ponce, Eloy, Pieter van Dillewijn, Regina Michaela Wittich et Cayo Ramos. « WHOP, a Genomic Region Associated With Woody Hosts in the Pseudomonas syringae Complex Contributes to the Virulence and Fitness of Pseudomonas savastanoi pv. savastanoi in Olive Plants ». Molecular Plant-Microbe Interactions® 30, n^o 2 (février 2017) : 113–26. http://dx.doi.org/10.1094/mpmi-11-16-0233-r.

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Bacteria from the Pseudomonas syringae complex belonging to phylogroups 1 and 3 (PG1 and PG3, respectively) isolated from woody hosts share a genomic region herein referred to as WHOP (from woody host and Pseudomonas spp.), which is absent in strains infecting herbaceous organs. In this work, we show that this region is also encoded in P. syringae pv. actinidifoliorum (PG1) and six additional members of PG3, namely, Pseudomonas savastanoi pv. retacarpa, three P. syringae pathovars, Pseudomonas meliae, and Pseudomonas amygdali. Partial conservation of the WHOP occurs in only a few PG2 strains. In P. savastanoi pv. savastanoi NCPPB 3335, the WHOP region is organized into four operons and three independently transcribed genes. While the antABC and catBCA operons mediate the catabolism of anthranilate and catechol, respectively, the ipoABC operon confers oxygenase activity to aromatic compounds. The deletion of antABC, catBCA, or ipoABC in NCPPB 3335 caused reduced virulence in woody olive plants without affecting knot formation in nonwoody plants; catBCA, dhoAB, and PSA3335_3206 (encoding a putative aerotaxis receptor) were also required for the full fitness of this strain exclusively in woody olive plants. Overall, this study sheds light on the evolution and adaptation of bacteria from the P. syringae complex to woody hosts and highlights the enzymatic activities encoded within the WHOP region that are essential for this process.

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Elmagdub, Fathea E., Nuria A. Elamri et Abdunabi M. Abughania. « Plasmid Profiles of Pseudomonas syringae pv. savastanoi. » Journal of Misurata University for Agricultural Sciences, n^o 01 (6 octobre 2019) : 354–78. http://dx.doi.org/10.36602/jmuas.2019.v01.01.28.

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Pseudomonas syringae pv. savastanoi (Psv) is the causal agent of olive knot disease. Forty nine bacterial isolates of Psv were isolated from knots on several hosts at the western area of Libya: 31 isolates from olive Olea europaea, 17 isolates from athel Tamarix aphylla (on which the disease is documented for the first time) and one isolate from retem Retama raetam. The isolates were identified on the basis of their morphological characteristics and LOPAT profile. They produced round, white creamy colonies on selective media (PAB and KB), from which 15 isolates produced fluorescent pigments. With the exception of other LOPAT analysis, all isolates were pectinolytic activity and arginine dihydrolase negative. some isolates were levan positive (10 isolates) and oxidase positive (12 isolates), while the rest of isolates were negative for both tests. Most of the isolates induced a hypersensitive reaction on tobacco and pepper leaves. Plasmid profile analysis of Psv strains indicated high genetic variability between the isolates of the same host or different hosts. Most of the olive isolates were classified according to their plasmid profile into five groups (A, B, C, D, F), however, the athel isolates were separated into three different groups designated as G, K, N, on the other hand, group E and H contained mixed isolates from different hosts: group H included two isolates from olive (OS25w and OS42w) and one isolate from retem (Ra1); only two strains OS6w and Ta5y from olive and athel respectively were classified within the same group designated as E. The remaining seven isolates from all hosts were unique. The total number of plasmids ranged from 1-4 for the strains tested, while the DNA content varied widely ranging from 540 to 13550 bp. No plasmid were detected in 14 isolates tested. Genome analysis based on plasmid profiles indicated the great potential of this technique to discriminate between the isolates of Psv from different hosts and geographical regions.

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Matas, Isabel M., M. Pilar Castañeda-Ojeda, Isabel M. Aragón, María Antúnez-Lamas, Jesús Murillo, Pablo Rodríguez-Palenzuela, Emilia López-Solanilla et Cayo Ramos. « Translocation and Functional Analysis of Pseudomonas savastanoi pv. savastanoi NCPPB 3335 Type III Secretion System Effectors Reveals Two Novel Effector Families of the Pseudomonas syringae Complex ». Molecular Plant-Microbe Interactions® 27, n^o 5 (mai 2014) : 424–36. http://dx.doi.org/10.1094/mpmi-07-13-0206-r.

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Pseudomonas savastanoi pv. savastanoi NCPPB 3335 causes olive knot disease and is a model pathogen for exploring bacterial infection of woody hosts. The type III secretion system (T3SS) effector repertoire of this strain includes 31 effector candidates plus two novel candidates identified in this study which have not been reported to translocate into plant cells. In this work, we demonstrate the delivery of seven NCPPB 3335 effectors into Nicotiana tabacum leaves, including three proteins from two novel families of the P. syringae complex effector super-repertoire (HopBK and HopBL), one of which comprises two proteins (HopBL1 and HopBL2) that harbor a SUMO protease domain. When delivered by P. fluorescens heterologously expressing a P. syringae T3SS, all seven effectors were found to suppress the production of defense-associated reactive oxygen species. Moreover, six of these effectors, including the truncated versions of HopAA1 and HopAZ1 encoded by NCPPB 3335, suppressed callose deposition. The expression of HopAZ1 and HopBL1 by functionally effectorless P. syringae pv. tomato DC3000D28E inhibited the hypersensitive response in tobacco and, additionally, expression of HopBL2 by this strain significantly increased its competitiveness in N. benthamiana. DNA sequences encoding HopBL1 and HopBL2 were uniquely detected in a collection of 31 P. savastanoi pv. savastanoi strains and other P. syringae strains isolated from woody hosts, suggesting a relevant role of these two effectors in bacterial interactions with olive and other woody plants.

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Moretti, Chiaraluce, Taha Hosni, Katrien Vandemeulebroecke, Carrie Brady, Paul De Vos, Roberto Buonaurio et Ilse Cleenwerck. « Erwinia oleae sp. nov., isolated from olive knots caused by Pseudomonas savastanoi pv. savastanoi ». International Journal of Systematic and Evolutionary Microbiology 61, n^o 11 (1 novembre 2011) : 2745–52. http://dx.doi.org/10.1099/ijs.0.026336-0.

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Three endophytic bacterial isolates were obtained in Italy from olive knots caused by Pseudomonas savastanoi pv. savastanoi. Phenotypic tests in combination with 16S rRNA gene sequence analysis indicated a phylogenetic position for these isolates in the genera Erwinia or Pantoea, and revealed two other strains with highly similar 16S rRNA gene sequences (>99 %), CECT 5262 and CECT 5264, obtained in Spain from olive knots. Rep-PCR DNA fingerprinting of the five strains from olive knots with BOX, ERIC and REP primers revealed three groups of profiles that were highly similar to each other. Multilocus sequence analysis (MLSA) based on concatenated partial atpD, gyrB, infB and rpoB gene sequences indicated that the strains constituted a single novel species in the genus Erwinia. The strains showed general phenotypic characteristics typical of the genus Erwinia and whole genome DNA–DNA hybridization data confirmed that they represented a single novel species of the genus Erwinia. The strains showed DNA G+C contents ranging from 54.7 to 54.9 mol%. They could be discriminated from phylogenetically related species of the genus Erwinia by their ability to utilize potassium gluconate, l-rhamnose and d-arabitol, but not glycerol, inositol or d-sorbitol. The name Erwinia oleae sp. nov. (type strain DAPP-PG 531T = LMG 25322T = DSM 23398T) is proposed for this novel taxon.

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Salman, Mazen, Rachel Greenhut, John Preece, Louise Ferguson et Daniel Kluepfel. « Field evaluation of olive (Olea europaea) genotypes for resistance to Pseudomonas savastanoi pv. savastanoi ». Journal of Plant Pathology 102, n^o 3 (25 mai 2020) : 663–70. http://dx.doi.org/10.1007/s42161-020-00549-8.

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Silverstone, Sara E., David G. Gilchrist, Richard M. Bostock et Tsune Kosuge. « The 73-kb pIAA plasmid increases competitive fitness of Pseudomonas syringae subspecies savastanoi in oleander ». Canadian Journal of Microbiology 39, n^o 7 (1 juillet 1993) : 659–64. http://dx.doi.org/10.1139/m93-095.

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Pseudomonas syringae subsp. savastanoi causes tumors on olive and oleander by producing the plant growth regulators indoleacetic acid (IAA) and cytokinins following infection of the plant. The contribution of IAA production to the ability of P. syringae subsp. savastanoi to grow and survive in oleander leaf tissue was studied. Bacterial strains differing only with respect to IAA production were characterized. Growth and survival of wild-type and two mutant strains of P. syringae subsp. savastanoi in oleander leaf tissue were monitored by weekly colony counts and IAA plate assays. Growth rate of the three strains in culture and in planta did not differ significantly. However, the wild-type strain reached a higher population density and maintained its maximum density at least 9 weeks longer than either mutant population. An insertion mutant containing the IAA plasmid (pIAA), but incapable of IAA production, did not maintain a higher population density than a strain cured of the IAA plasmid. The pIAA-cured strain maintained a higher population density when coinoculated with an IAA-producing strain than when inoculated alone. These results suggest that IAA production may contribute to the fitness of P. syringae subsp. savastanoi in oleander tissue and that the iaa operon alone may be responsible for the competitive advantage of cells harboring pIAA.Key words: indoleacetic acid, bacterial ecology.

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Soby, Scott, Bruce Kirkpatrick et Tsune Kosuge. « Chemotaxis of Pseudomonas syringae subsp. savastanoi Virulence Mutants ». Applied and Environmental Microbiology 57, n^o 10 (1991) : 2918–20. http://dx.doi.org/10.1128/aem.57.10.2918-2920.1991.

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Temsah, M., L. Hanna et A. T. Saad. « Anatomical observations of Pseudomonas savastanoi on Rhamnus alaternus ». Forest Pathology 37, n^o 1 (février 2007) : 64–72. http://dx.doi.org/10.1111/j.1439-0329.2007.00483.x.

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Wells, J. M., F. J. Casano et G. Surico. « Fatty Acid Composition of Pseudomonas syringae pv. savastanoi ». Journal of Phytopathology 133, n^o 2 (octobre 1991) : 152–62. http://dx.doi.org/10.1111/j.1439-0434.1991.tb00148.x.

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Ghanney, Nadia, et Ali Ferchichi. « Anatomical pathogenesis and histological interaction between Pseudomonas savastanoi pv. savastanoi strain KT11 and Pseudomonas fluorescens strain PICF4 in olive knots ». Journal of Plant Pathology 101, n^o 4 (17 juin 2019) : 1025–34. http://dx.doi.org/10.1007/s42161-019-00333-3.

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Gardan, L., C. Bollet, M. Abu Ghorrah, F. Grimont et P. A. D. Grimont. « DNA Relatedness among the Pathovar Strains of Pseudomonas syringae subsp. savastanoi Janse (1982) and Proposal of Pseudomonas savastanoi sp. nov. » International Journal of Systematic Bacteriology 42, n^o 4 (1 octobre 1992) : 606–12. http://dx.doi.org/10.1099/00207713-42-4-606.

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Glickmann, Eric, Louis Gardan, Sylvie Jacquet, Shafik Hussain, Miena Elasri, Annik Petit et Yves Dessaux. « Auxin Production Is a Common Feature of Most Pathovars of Pseudomonas syringae ». Molecular Plant-Microbe Interactions® 11, n^o 2 (février 1998) : 156–62. http://dx.doi.org/10.1094/mpmi.1998.11.2.156.

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We investigated indole-3-acetic acid (IAA) production by 57 pathovars of Pseudomonas syringae and related species. Most of those analyzed produced IAA, especially in the presence of tryptophan. Eight strains produced high IAA concentrations in the absence of Trp. The iaaM and iaaH genes of P. savastanoi pv. savastanoi were detected in a limited number of strains only, including the eight above-mentioned strains. Thus, IAA synthesis in most assayed strains of P. syringae and related species does not involve genes highly similar to iaaM and iaaH. In contrast, the iaaL gene encoding an IAA-lysine synthase was detected in most pathovars, and was often found on plasmids.

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Harmon, Carrie Lapaire, Sujan Timilsina, John Bonkowski, Debra D. Jones, Xiaoan Sun, Gary E. Vallad, Laura Ramos Sepulveda, Carolee Bull et Jeffrey B. Jones. « Bacterial Gall of Loropetalum chinense caused by Pseudomonas amygdali pv. loropetali pv. nov. » Plant Disease 102, n^o 4 (avril 2018) : 799–806. http://dx.doi.org/10.1094/pdis-04-17-0505-re.

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In 2012, stem gall samples on Loropetalum chinense were sent to Florida diagnostic labs from Alabama and Florida nurseries. A fluorescent pseudomonad was consistently isolated from the galls. The organism was originally identified in Alabama based on 16S rRNA sequencing as Pseudomonas savastanoi, which causes a production-limiting disease of olive. The loropetalum strains and reference strains were compared using LOPAT, Biolog, fatty acid analysis, multilocus sequence analysis (MLSA), and pathogenicity tests. The LOPAT tests placed the loropetalum strains within Pseudomonas syringae. Biolog and fatty acid analysis placed the strains in various pathovars of P. syringae and P. savastanoi, respectively. MLSA of a set of housekeeping genes separated the loropetalum strains from the olive knot-inducing strains. Our work indicates there is a need to use more tests than 16S rRNA to accurately diagnose new bacterial diseases. In pathogenicity tests, the loropetalum strains produced galls only on loropetalum, but not on olive, mandevilla, or almond, indicating this strain is not a threat to the olive industry. Based on the pathogenicity assays and molecular tests, loropetalum strains represent a distinct and new pathovar, P. amygdali pv. loropetali pv. nov., for which the strain PDC13-208 (= DSMZ 105780PT) has been designated as the pathotype strain.

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Penyalver, R., A. García, A. Ferrer, E. Bertolini, J. M. Quesada, C. I. Salcedo, J. Piquer et al. « Factors Affecting Pseudomonas savastanoi pv. savastanoi Plant Inoculations and Their Use for Evaluation of Olive Cultivar Susceptibility ». Phytopathology® 96, n^o 3 (mars 2006) : 313–19. http://dx.doi.org/10.1094/phyto-96-0313.

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Pseudomonas savastanoi pv. savastanoi causes olive knot disease, which is present in most countries where olive trees are grown. Although the use of cultivars with low susceptibility may be one of the most appropriate methods of disease control, little information is available from inoculation assays, and cultivar susceptibility assessments have been limited to few cultivars. We have evaluated the effects of pathogen virulence, plant age, the dose/response relationship, and the induction of secondary tumors in olive inoculation assays. Most P. savastanoi pv. savastanoi strains evaluated were highly virulent to olive plants, but interactions between cultivars and strains were found. The severity of the disease in a given cultivar was strongly dependent of the pathogen dose applied at the wound sites. Secondary tumors developed in noninoculated wounds following inoculation at another position on the stem, suggesting the migration of the pathogen within olive plants. Proportion and weight of primary knots and the presence of secondary knots were evaluated in 29 olive cultivars inoculated with two pathogen strains at two inoculum doses, allowing us to rate most of the cultivars as having either high, medium, or low susceptibility to olive knot disease. None of the cultivars were immune to the disease.

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Tsuji, Mizue, Kouki Ohta, Kouta Tanaka et Yuichi Takikawa. « Comparison among Japanese isolates of Pseudomonas savastanoi pv. savastanoi, causal agent of olive knot disease ». Journal of General Plant Pathology 83, n^o 3 (10 avril 2017) : 152–61. http://dx.doi.org/10.1007/s10327-017-0710-2.

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Sisto, Angelo, Maria Grazia Cipriani, Maria Morea, Stella Lisa Lonigro, Francesca Valerio et Paola Lavermicocca. « An Rhs-like genetic element is involved in bacteriocin production by Pseudomonas savastanoi pv. savastanoi ». Antonie van Leeuwenhoek 98, n^o 4 (20 juin 2010) : 505–17. http://dx.doi.org/10.1007/s10482-010-9468-7.

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Arvizu-Gómez, Jackeline Lizzeta, Alejandro Hernández-Morales, Rafael Arnulfo Juárez-Navarro, Juan Diego Paredes-Tadeo, Juan Campos-Guillén, Juan Ramiro Pacheco-Aguilar, Abril Bernardette Martínez-Rizo et Christian González-Reyes. « OxyR Positively Influences Phaseolotoxin Synthesis and Pyoverdin Production in Pseudomonas savastanoi pv. phaseolicola NPS3121 ». Microorganisms 10, n^o 11 (27 octobre 2022) : 2123. http://dx.doi.org/10.3390/microorganisms10112123.

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Phaseolotoxin is a major virulence factor of the bean pathogen bacterium P. savastanoi pv. phaseolicola. This toxin plays a key role in the development of the halo blight disease in bean plants. So far, the signal transduction pathways involved in the synthesis of phaseolotoxin have not been elucidated. The influence of regulation mechanisms related to the oxidative stress response, in particular the OxyR protein, it has been suggested to be involved in this process.. In this study we evaluated the role of OxyR in P. savastanoi pv. phaseolicola, mainly compared to the synthesis of phaseolotoxin and the virulence of this phytopathogen. Generation of the oxyR-mutant, pathogenicity and virulence tests, and analyses of gene expression by RT-PCR assays were performed. The results showed that OxyR exerts an effect on the synthesis of phaseolotoxin and positively influences the expression of the Pht and Pbo cluster genes. Likewise, OxyR influences the production of pyoverdine by the control of the expression of the genes encoding the PvdS sigma factor, involved in the synthesis of this pigment. This study is the first report on members of the OxyR regulon of P. savastanoi pv. phaseolicola NPS3121.

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Cinelli, T., D. Rizzo, G. Marchi et G. Surico. « First Report of Knot Disease Caused by Pseudomonas savastanoi on Sweet Olive in Central Italy ». Plant Disease 97, n^o 3 (mars 2013) : 419. http://dx.doi.org/10.1094/pdis-09-12-0818-pdn.

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In April 2012 the presence of hyperplastic outgrowths on trunks, branches, and twigs of sweet olive plants, Osmanthus fragrans Lour (Fam. Oleaceae), was recorded in two ornamental hedges made up of five and four plants, respectively, in the city center of Montecatini (Pistoia-Italy). All sweet olive plants were seriously affected by the disease with outgrowths appearing either singly or close together, often forming a single mass that could extend up to 20 cm along the stems, occasionally surrounding the entire circumference. The symptoms observed on O. fragrans closely resembled those induced by the bacterium Pseudomonas savastanoi on Olea europea (common olive) and other plant species. Suspecting a bacterial origin of the disorder, young knots were collected from four diseased plants and used for bacterial isolation with standard techniques on nutrient sucrose agar medium (1). After 3 days of incubation at 26°C, non-levan forming colonies about 3 mm in diameter that were circular, convex, smooth, and cream colored with entire margins appeared on the surface of the agar medium. Purified isolates were gram negative, levan negative, oxidase negative, potato rot negative, arginine dihydrolase negative, showed a tobacco hypersensitive reaction, and tested positive to PCR screening for the presence of the iaaM (tryptophan-2-monooxygenase), iaaH (indoleacetamide hydrolase), ptz (isopentenyl transferase) (1) and iaaL (IAA-lysine synthethase) (3) genes. Three isolates were selected arbitrarily and further characterized by sequencing a fragment of the housekeeping genes rpoD (sigma factor 70) and pgi (phosphoglucose isomerase) (2). All sequenced gene fragments, of 620 bp and 552 bp for the rpoD and pgi genes, respectively, were identical to those of P. savastanoi pv. savastanoi strain NCPPB3335. The pathogenicity of the three isolates was verified on three O. fragrans plants and three Olea europea (cv. Frantoio) plants. Per each isolate, three 1-cm wounds were made on the branches of each plant using a sterile scalpel dipped in a bacterial suspension (1 × 108 CFU/ml). P. savastanoi pv. savastanoi PVFi-t2b isolated from olive was also inoculated as reference strain. After 30 days, all isolates including the reference strain induced typical knots on both plant species while no symptoms were observed on wounds inoculated with sterile water. Bacteria were reisolated from induced knots and Koch's postulates were confirmed. On the basis of biochemical tests, PCR screening, pathogenicity testing, and sequence analyses, the causal agent of knot disease on O. fragrans was identified as P. savastanoi. The potential susceptibility of O. aquifolium Sieb. to the causal agent of olive knot disease has been demonstrated in the past by means of artificial inoculations but interestingly, in the same trials, O. fragrans had tested negative (4). To the best of our knowledge, this is the world's first report of O. fragrans as natural host of P. savastanoi, which extends the growing list of cultivated and ornamental plant species affected by this phytopathogenic bacterium. References: (1) G. Marchi et al. Eur J. Plant Pathol. 112:101, 2005. (2) N. Parkinson et al. Plant Pathol. 60:338, 2011. (3) R. Penyalver et al. Appl. Environ. Microbiol. 66:2673, 2000. (4) C. O. Smith. Phytopathology 12:271, 1922.

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Schaad, N. W., A. K. Vidaver, G. H. Lacy, K. Rudolph et J. B. Jones. « Evaluation of Proposed Amended Names of Several Pseudomonads and Xanthomonads and Recommendations ». Phytopathology® 90, n^o 3 (mars 2000) : 208–13. http://dx.doi.org/10.1094/phyto.2000.90.3.208.

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In 1980, over 90% of all plant-pathogenic pseudomonads and xanthomonads were lumped into Pseudomonas syringae and Xanthomonas campestris, respectively, as pathovars. The term “pathovar” was created to preserve the name of plant pathogens, but has no official standing in nomenclature. Proposals to elevate and rename several pathovars of the genera Pseudomonas and Xanthomonas to the rank of species has caused great confusion in the literature. We believe the following changes have merit and expect to adopt them for publication in a future American Phytopathological Society Laboratory Guide for Identification of Plant Pathogenic Bacteria. Upon review of published data and the Rules of The International Code of Nomenclature of Bacteria, we make the following recommendations. We reject the proposal to change the name of P. syringae pvs. phaseolicola and glycinea to P. savastanoi pvs. phaseolicola and glycinea, respectively, because both pathogens are easily differentiated phenotypically from pv. savastanoi and convincing genetic data to support such a change are lacking. We accept the elevation of P. syringae pv. savastanoi to the rank of species. We accept the reinstatement of X. oryzae to the rank of species with the inclusion of X. oryzicola as a pathovar of X. oryzae and we accept the species X. populi. We agree with the elevation of the pvs. cassavae, cucurbitae, hyacinthi, pisi, and translucens to the rank of species but not pvs. melonis, theicola, and vesicatoria type B. We recommend that all type A X. vesicatoria be retained as X. campestris pv. vesicatoria and all type B X. vesicatoria be named X. exitiosa. We reject the newly proposed epithets arboricola, bromi, codiaei (poinsettiicola type B), hortorum, sacchari, and vasicola and the transfer of many pathovars of X. campestris to X. axonopodis. The proposed pathovars of X. axonopodis should be retained as pathovars of X. campestris.

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Sisto, A., M. G. Cipriani, S. Tegli, M. Cerboneschi, G. Stea et E. Santilli. « Genetic characterization by fluorescent AFLP of Pseudomonas savastanoi pv. savastanoi strains isolated from different host species ». Plant Pathology 56, n^o 3 (5 février 2007) : 366–72. http://dx.doi.org/10.1111/j.1365-3059.2007.01567.x.

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Tarakanov, Rashit I., Anna A. Lukianova, Peter V. Evseev, Stepan V. Toshchakov, Eugene E. Kulikov, Alexander N. Ignatov, Konstantin A. Miroshnikov et Fevzi S. U. Dzhalilov. « Bacteriophage Control of Pseudomonas savastanoi pv. glycinea in Soybean ». Plants 11, n^o 7 (30 mars 2022) : 938. http://dx.doi.org/10.3390/plants11070938.

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Bacterial viruses (bacteriophages) have been considered as potential agents for the biological control of bacterial phytopathogens due to their safety and host specificity. Pseudomonas savastanoi pv. glycinea (Psg) is a causative agent of the bacterial spotting of soybean (Glycine max Willd). The harm caused by this bacterium to crop production and the development of antibiotic resistance in Psg and other pathogenic microorganisms has led to the pursuit of alternative management strategies. In this study, three Psg-specific lytic bacteriophages were isolated from soybean field soil in geographically distant regions of Russia, and their potential for protective action on plants was assessed. Sequencing of phage genomes has revealed their close relatedness and attribution to the genus Ghunavirus, subfamily Studiervirinae, family Autographiviridae. Extensive testing of the biological properties of P421, the representative of the isolated phage group, has demonstrated a relatively broad host range covering closely related Pseudomonas species and stability over wide temperature (4–40 °C) and pH (pH 4–7) ranges, as well as stability under ultraviolet irradiation for 30 min. Application of the phages to prevent, and treat, Psg infection of soybean plants confirms that they are promising as biocontrol agents.

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Kheira, Benyoub, Kacem Mourad et Kaid-Harche Meriem. « Detection of Pseudomonas syringae pv. Savastanoi, causal agent of olive tuberculosis in two regions of Western Algeria (Ain TÃ©mouchent and Sig) ». South Asian Journal of Experimental Biology 9, n^o 2 (26 septembre 2019) : 64–71. http://dx.doi.org/10.38150/sajeb.9(2).p64-71.

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The present study on olive tuberculosis commenced by isolating bacteria of the genus Pseudomonas from the soils and necrosis of collected olive trees. A total of 180 samples were used in this study: (100) rhizospheric soil samples: (60) samples at the region of Ain Témouchent and (40) at the region of Sig in western of Algeria. In total, (80) galls were collected (40) at branch level and (40) galls at olive tree leaf (level). The isolates were identified by microbiological (macroscopic and microscopic examination), physiological (growth in the presence of Salt (NaCl), growth at different pH values and growth at different temperatures) and biochemical methods (the LOPAT and Galeries Api 20 NE test to identify species of the Pseudomonas group and conventional biochemical tests to identify the subspecies P. syringae pv. Savastanoi).This allowed to identify 110 isolates of Pseudomonas (60 isolates of P. aeruginosa, 35 isolates of P. fluorescens and 15 isolates of P. syringae pv Savastanoi the causal agent of olive node disease) which are now part of the collection of Pseudomonas bacteria of the laboratory of the Biotechnology Department (USTO-MB). The selection of technological performance isolates useful for our agriculture could solve phytopathological problems and finally constitute a collection of the bacteria preserved.

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FİLİZ DOKSÖZ, Senem, et İmam Adem BOZKURT. « Biological control of Pseudomonas savastanoi pv. savastanoi causing the olive knot disease with epiphytic and endophytic bacteria ». Journal of Plant Pathology 104, n^o 1 (16 novembre 2021) : 65–78. http://dx.doi.org/10.1007/s42161-021-00975-2.

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Hall, B. H., E. J. Cother, M. Whattam, D. Noble, J. Luck et D. Cartwright. « First report of olive knot caused by Pseudomonas savastanoi pv. savastanoi on olives (Olea europaea) in Australia ». Australasian Plant Pathology 33, n^o 3 (2004) : 433. http://dx.doi.org/10.1071/ap04031.

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Tsuji, M., K. Ohta, K. Tanaka et Y. Takikawa. « First Report of Knot Disease on Olive (Olea europaea) in Japan Caused by Pseudomonas savastanoi pv. savastanoi ». Plant Disease 99, n^o 10 (octobre 2015) : 1445. http://dx.doi.org/10.1094/pdis-12-14-1318-pdn.

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Mustafa ; AYSAN, MIRIK. « Marmara Bölgesinde zeytin dal kanseri hastalığının yaygınlığı ve Pseudomonas savastanoi pv. savastanoi izolatlarının fenotipik ve genotipik karakterizasyonu ». Tarım Bilimleri Dergisi 17, n^o 4 (2011) : 279–90. http://dx.doi.org/10.1501/tarimbil_0000001180.

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Lamichhane, Jay Ram, et Leonardo Varvaro. « Epiphytic Pseudomonas savastanoi pv. savastanoi can infect and cause olive knot disease on Olea europaea subsp. cuspidata ». Australasian Plant Pathology 42, n^o 2 (23 octobre 2012) : 219–25. http://dx.doi.org/10.1007/s13313-012-0171-1.

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Pirc, M., M. Ravnikar et T. Dreo. « First Report of Pseudomonas savastanoi Causing Bacterial Leaf Spot of Mandevilla sanderi in Slovenia ». Plant Disease 99, n^o 3 (mars 2015) : 415. http://dx.doi.org/10.1094/pdis-07-14-0672-pdn.

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Foliar necrotic spots with narrow chlorotic halos were observed on different cultivars of Brazilian Jasmine (Mandevilla sanderi) during spring 2010 in several commercial greenhouses in Slovenia. Up to 70% were symptomatic and were unmarketable. No galls were observed on the stems of symptomatic plants. Circular, flat, granulated colonies with entire margins were isolated from symptomatic leaves of two plants from different greenhouses on King's B medium (KB). The isolates were negative for levan, oxidase, pectinolytic and arginine dihydrolase activity. They caused a hypersensitive reaction on tomato but not on tobacco cv. White Burley. Isolates were weakly fluorescent on KB under UV light. One isolate per sample (NIB Z 1413 and 1415) was further characterized. Partial sequences of 16S rDNA (1; GenBank KM603318 of 722 bp, KM603319 of 686 bp) grouped the isolates within genomospecies 2 of Pseudomonas. Repetitive polymerase chain reaction (PCR) assay using the BOXA1R primer (5) resulted in highly similar DNA fragment banding patterns of the two NIB Z isolates and other reference strains of genomospecies 2 (minimum 95.1% identity with Pearson's correlation). Partial sequences of rpoD (3) of the two Slovenian isolates (600 bp; GenBank KJ744202, KJ744201) were identical to the P. savastanoi isolate from Mandevilla B200 (W. Wohanka, Germany; GenBank KJ744203) and P. s. pv. nerii strain NCPPB 3334 (GenBank AB039513). The sequences differed in two nucleotides relative to the sequence of the pathotype strain of pv. nerii NCPPB 3278 (positions 487 and 510 relative to GenBank FN433279) and had an insertion of six nucleotides compared to available P. savastanoi pv. savastanoi rpoD sequences (NZ_JOJV01000073, CM001834). Pathogenicity of isolated bacteria (two isolates) was determined on M. sanderi cv. Pretty Rose inoculated by two different methods, spraying foliage and pricking stems. The abaxial and adaxial surfaces of leaves were sprayed with a 30-ml bacterial suspension (5 × 106 CFU/ml). Three plants were inoculated with each isolate: NIB Z 1413 and 1415 and the reference strain NCPPB 3278. Necrotic spots developed on leaves after 14 days of incubation, under >80% high relative humidity, with 16 h of daylight at 25°C and 8 h of dark at 21°C. One month after inoculation, necrosis also developed on stems and new growth. Inoculation of bacteria by pricking nodes of healthy M. sanderi cv. Pretty Rose with a needle dipped in the isolates grown on KB for 24 h (each of NIB Z 1413, 1415, and NCPPB 3278 for positive control) led to development of galls in 14 days at the inoculation points. The re-isolation was performed separately from necrotic spots on leaves, stems, new growth above the inoculation points, and galls. The BOX-PCR profiles of the bacteria isolated from symptomatic tissues were identical to the original profiles, thus confirming the systemic spread of the bacteria. None of the three negative control plants sprayed with 0.01M MgSO4 or pricked with a sterile needle developed symptoms. This is the first report of P. savastanoi on Mandevilla sanderi plants in greenhouse production in Slovenia. The galls caused by P. savastanoi have previously been reported from the United States (4) and Germany (2). This report broadens the geographical area where P. savastanoi, causing both galls on stems and necrotic spots on leaves, can be found in commercial production of Mandevilla spp. References: (1) U. Edwards et al. Nucleic Acids Res. 17:7843, 1989. (2) N. Eltlbany et al. Appl. Environ. Microbiol. 78:8492, 2012. (3) N. Parkinson et al. Plant Pathol. 60:338, 2011. (4) M. L. Putnam et al. Phytopathology 100:S104, 2010. (5) J. Versalovic et al. Methods Mol. Cell Biol. 5:25, 1994.

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Izadiyan, Mahsa, S. Mohsen Taghavi et Parisa Izadiyan. « Application of counter propagation artificial neural network for classification and genetic diversity assessment of somePseudomonasspecies ». Journal of Theoretical and Computational Chemistry 14, n^o 06 (septembre 2015) : 1550042. http://dx.doi.org/10.1142/s021963361550042x.

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Members of the genus Pseudomonas bacterium are of great interest because of their importance in plant disease. In this study, DNA fingerprints of 60 strains of Pseudomonas bacteria including three species of Pseudomonas syringae (Pseudomonas syringae pv. syringae (Pss) and Pseudomonas syringae pv. Lachrymans (Psl)), Pseudomonas savastanoi (Psa) and Pseudomonas tolaasii (Pt) were used for developing a robust predictive classification model. The DNA fingerprints were obtained by repetitive polymerase chain reaction (Rep-PCR) using enterobacterial repetitive intergenic consensus (ERIC), repetitive extragenic palindromes (REP), and BOXAIR primers. The classification results of counter propagation artificial neural network (CP-ANN) modeling indicated that a combination of Rep-PCR fingerprinting and chemometrics analysis can be used as an effective and powerful methodology to differentiate species of Pseudomonas and pathovars of P. syringae strains based on a predictive model.

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Wang, Jingru, Xiaolong Shao, Yingchao Zhang, Yanan Zhu, Pan Yang, Jian Yuan, Tingting Wang et al. « HrpS Is a Global Regulator on Type III Secretion System (T3SS) and Non-T3SS Genes in Pseudomonas savastanoi pv. phaseolicola ». Molecular Plant-Microbe Interactions® 31, n^o 12 (décembre 2018) : 1232–43. http://dx.doi.org/10.1094/mpmi-02-18-0035-r.

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The type III secretion system (T3SS) is the main machinery for Pseudomonas savastanoi and other gram-negative bacteria to invade plant cells. HrpR and HrpS form a hetero-hexamer, which activates the expression of HrpL, which induces all T3SS genes by binding to a ‘hrp box’ in promoters. However, the individual molecular mechanism of HrpR or HrpS has not been fully understood. Through chromatin immunoprecipitation coupled to high-throughput DNA sequencing, we found that HrpR, HrpS, and HrpL had four, 47, and 31 targets on the genome, respectively. HrpS directly bound to the promoter regions of a group of T3SS genes and non-T3SS genes. HrpS independently regulated these genes in a hrpL deletion strain. Additionally, a HrpS-binding motif (GTGCCAAA) was identified, which was verified by electrophoretic mobility shift assay and lux-reporter assay. HrpS also regulated motility and biofilm formation in P. savastanoi. The present study strongly suggests that HrpS alone can work as a global regulator on both T3SS and non-T3SS genes in P. savastanoi. [Formula: see text] Copyright © 2018 The Author(s). This is an open-access article distributed under the CC BY-NC-ND 4.0 International license .

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Cho, Jung-Hee, Min-Jung Jeong, Min-Ji Song, Kyu-Ock Yim, Hyok-In Lee, Jung-Hee Kim, Ji-Hyun Baeg et Jae-Soon Cha. « Development of PCR Primers to Detect Pseudomonas savastanoi pv. phaseolicola from the Bean Seeds ». Research in Plant Disease 16, n^o 2 (1 août 2010) : 129–35. http://dx.doi.org/10.5423/rpd.2010.16.2.129.

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Fernandes, A., et M. Marcelo. « EVALUATION OF THE SENSITIVITY OF PSEUDOMONAS SAVASTANOI TO SEVENTEEN ANTIBIOTICS ». Acta Horticulturae, n^o 791 (juin 2008) : 565–68. http://dx.doi.org/10.17660/actahortic.2008.791.87.

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Iacobellis, N. S., A. Caponero et A. Evidente. « Characterization of Pseudomonas syringae ssp. savastanoi strains isolated from ash ». Plant Pathology 47, n^o 1 (février 1998) : 73–83. http://dx.doi.org/10.1046/j.1365-3059.1998.00202.x.

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Ustun, Nursen. « Virulence and indole-3-acetic acid (IAA) biosynthesis ability of Turkish Pseudomonas savastanoi pv. savastanoi isolates and susceptibility of some native olive genotypes ». Spanish Journal of Agricultural Research 19, n^o 4 (décembre 2021) : e1003-e1003. http://dx.doi.org/10.5424/sjar/2021194-17492.

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Aim of study: To evaluate the virulence and indole-3-acetic acid (IAA) biosynthesis ability of several Turkish P. savastanoi pv. savastanoi isolates and the susceptibility of some native genotypes to olive knot. Area of study: The Aegean, Marmara, and Mediterranean Regions of Turkey. Material and methods: 101 isolated bacteria were identified on the basis of biochemical, PCR for amplification of the bacterial iaaL gene, and pathogenicity tests. The virulence of the isolates was determined in a randomized experimental trial carried out by stem inoculation of pot-grown seedlings of olive (cv. ‘Manzanilla’) in the growing chamber. The amounts of IAA produced by the isolates were determined colorimetrically. The susceptibility of native olive genotypes was evaluated on 2-yr old plants inoculated with two distinct strains. Main results: Tested P. savastanoi pv. savastanoi isolates showed significant differences in virulence found to be associated with their geographical origin. The isolates produced IAA amounts varied from 148.67 to 0.3 μg mL-1. The geographical variation in IAA biosynthesis ability of the isolates was observed. No correlation (R=0.0225) was determined between virulence and IAA amounts of the isolates. Native olive genotypes indicated different susceptibility levels to the olive knot pathogen. No genotype tested had complete resistance. However, low susceptible genotypes (‘Memecik’, ‘Ayvalık’ and ‘Uslu’) were identified. Some genotypes had variable reactions depending on the isolate used. Research highlights: The results undergird the differences in the virulence and IAA production of the isolates within the area and also between geographical locations. Genotypes with low susceptibility can be used as genitors in further breeding studies.

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Bouaichi, Abdelaaziz. « POTENTIAL EFFECT OF ANTAGONISTIC BACTERIA IN THE MANAGEMENT OF OLIVE KNOT DISEASE CAUSED BY PSEUDOMONAS SAVASTANOI PV. SAVASTANOI ». Journal of Microbiology, Biotechnology and Food Sciences 8, n^o 4 (février 2019) : 1035. http://dx.doi.org/10.15414/jmbfs.2019.8.4.1035-1040.

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Nguyen, K. A., H. Förster et J. E. Adaskaveg. « Genetic Diversity of Pseudomonas savastanoi pv. savastanoi in California and Characterization of Epidemiological Factors for Olive Knot Development ». Plant Disease 102, n^o 9 (septembre 2018) : 1718–24. http://dx.doi.org/10.1094/pdis-11-17-1709-re.

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Olive knot, caused by Pseudomonas savastanoi pv. savastanoi, is a limiting disease in the production of table and oil olives in California. The genetic variability among 152 strains from major production areas of California was determined using BOX, ERIC, and REP primers in repetitive element sequence-based polymerase chain reaction. Overall genetic variability was low, and strains shared at least 82% similarity. Phenetic analyses identified several genotypes but most strains belonged to one of two major groups. Three copper-resistant strains had two fingerprints that were distinct from any of the sensitive strains, indicating that they may have been introduced from other production areas or hosts. In inoculations, two copper-resistant strains were mostly equally as virulent as two copper-sensitive strains. Inoculum was exuded at high levels (>108 CFU/g of knot tissue) within 10 min from hydrated olive knots, and concentrations were 2- to 3-log higher than the minimum needed to induce knot formation. Arbequina olive was significantly more susceptible to infection and developed a higher incidence of knots on leaf scar and lateral wounds (59.7 to 80.6% incidence) than Manzanillo (47.4 to 68.2% incidence). In wound-healing studies, both types of wounds were less susceptible to infection ≥10 days after injury, indicating a critical period for infection and application of bactericides during favorable environments.

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Varympopi, Adamantia, Anastasia Dimopoulou, Ioannis Theologidis, Theodora Karamanidou, Alexandra Kaldeli Kerou, Afroditi Vlachou, Dimitrios Karfaridis et al. « Bactericides Based on Copper Nanoparticles Restrain Growth of Important Plant Pathogens ». Pathogens 9, n^o 12 (5 décembre 2020) : 1024. http://dx.doi.org/10.3390/pathogens9121024.

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Copper nanoparticles (CuNPs) can offer an alternative to conventional copper bactericides and possibly slow down the development of bacterial resistance. This will consequently lower the accumulation rate of copper to soil and water and lower the environmental and health burden imposed by copper application. Physical and chemical methods have been reported to synthesize CuNPs but their use as bactericides in plants has been understudied. In this study, two different CuNPs products have been developed, CuNP1 and CuNP2 in two respective concentrations (1500 ppm or 300 ppm). Both products were characterized using Dynamic Light Scattering, Transmission Electron Microscopy, Attenuated Total Reflection measurements, X-ray Photoelectron Spectroscopy, X-ray Diffraction and Scattering, and Laser Doppler Electrophoresis. They were evaluated for their antibacterial efficacy in vitro against the gram-negative species Agrobacterium tumefaciens, Dickeya dadantii, Erwinia amylovora, Pectobacterium carotovorum, Pseudomonas corrugata, Pseudomonas savastanoi pv. savastanoi, and Xanthomonas campestris pv. campestris. Evaluation was based on comparisons with two commercial bactericides: Kocide (copper hydroxide) and Nordox (copper oxide). CuNP1 inhibited the growth of five species, restrained the growth of P. corrugata, and had no effect in X. c. pv campestris. MICs were significantly lower than those of the commercial formulations. CuNP2 inhibited the growth of E. amylovora and restrained growth of P. s. pv. savastanoi. Again, its overall activity was higher compared to commercial formulations. An extensive in vitro evaluation of CuNPs that show higher potential compared to their conventional counterpart is reported for the first time and suggests that synthesis of stable CuNPs can lead to the development of low-cost sustainable commercial products.

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Goychuk, А. F., I. M. Kulbanska et M. V. Shvets. « Bacteria Associated with Pseudomonas syringae pv. savastanoi in the Pathology of Fraxinus excelsior L. » Mikrobiolohichnyi Zhurnal 82, n^o 3 (17 juin 2020) : 22–34. http://dx.doi.org/10.15407/microbiolj82.03.022.

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Articles de revues sur le sujet « Pseudomonas savastanoi »

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