Thèses sur le sujet « Protein Mediated Membrane Fusion »
Créez une référence correcte selon les styles APA, MLA, Chicago, Harvard et plusieurs autres
Consultez les 50 meilleures thèses pour votre recherche sur le sujet « Protein Mediated Membrane Fusion ».
À côté de chaque source dans la liste de références il y a un bouton « Ajouter à la bibliographie ». Cliquez sur ce bouton, et nous générerons automatiquement la référence bibliographique pour la source choisie selon votre style de citation préféré : APA, MLA, Harvard, Vancouver, Chicago, etc.
Vous pouvez aussi télécharger le texte intégral de la publication scolaire au format pdf et consulter son résumé en ligne lorsque ces informations sont inclues dans les métadonnées.
Parcourez les thèses sur diverses disciplines et organisez correctement votre bibliographie.
Howard, Megan Wilder. « Coronavirus mediated membrane fusion / ». Connect to full text via ProQuest. Limited to UCD Anschutz Medical Campus, 2008. http://proquest.umi.com/pqdweb?did=1552538711&sid=1&Fmt=6&clientId=18952&RQT=309&VName=PQD.
Texte intégralTypescript. Includes bibliographical references (leaves 161-183). Free to UCD Anschutz Medical Campus. Online version available via ProQuest Digital Dissertations;
Mair, Caroline. « Membrane fusion mediated by the influenza virus hemagglutinin ». Doctoral thesis, Humboldt-Universität zu Berlin, Lebenswissenschaftliche Fakultät, 2015. http://dx.doi.org/10.18452/17217.
Texte intégralThe entry of influenza A virus into host cells is established by the hemagglutinin (HA) protein. New antiviral strategies aim to inhibit the fusion inducing conformational change of HA and thereby liberation of the viral genome into the cell. This process is strictly pH dependent since the conformational change of HA initiating the fusion of membranes only occurs upon protonation of yet unknown residues within HA at low pH (~5.0-6.0). The identification of conserved titrable residues and better understanding of the sequential structural rearrangements within HA may facilitate the development of new broad-spectrum antivirals. In the present work His184 and His110 were characterized as potential pH sensors by a comprehensive mutational and computational analysis. The results suggest that His184, but not His110, is an important regulator of HA conformational change at low pH. Furthermore, an exchange of charge at position 216 in vicinity to His184 was shown to alter the pH dependence of conformational change and of fusion in correlation to the known pKa dependence of histidines on neighboring residues. The result advocates that the mutation R216E, which emerged in the highly pathogenic H5 HA in 2003-2004, contributed to an altered acid stability of H5 HA via its effect on His184 and thus to the adaptation of avian H5N1 viruses to the human host. Therefore, the role of an altered acid stability of HA for viral fusion and infectivity in living cells was assessed. Recombinant viruses containing a destabilizing mutation in the HA protein were found to have a reduced infectivity and replication efficiency in MDCK cells compared to the respective wild type. Studying virus-endosome fusion kinetics in these cells we could resolve a significant difference in the timing of fusion induction suggesting that the time-point of fusion is a critical determinant of viral infection efficiency which depends on the endosomal acidification as well as on the acid stability of HA.
Liu, Tina Yu. « Mechanism of endoplasmic reticulum membrane fusion mediated by the Atlastin GTPase ». Thesis, Harvard University, 2014. http://nrs.harvard.edu/urn-3:HUL.InstRepos:13064987.
Texte intégralStone-Hulslander, Judith. « Mechanisms of Newcastle Disease Virus-Mediated Membrane Fusion : A Dissertation ». eScholarship@UMMS, 1999. https://escholarship.umassmed.edu/gsbs_diss/131.
Texte intégralAtfield, Rachel Sarah. « Herpes simplex virus glycoprotein-mediated membrane fusion ». Thesis, University of Cambridge, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.615860.
Texte intégralChen, Yong. « Structural and functional studies on SNAREs-mediated membrane fusion ». [Ames, Iowa : Iowa State University], 2006.
Trouver le texte intégralLu, Xiaobing. « Studies of intermediates and regulation in SNARE-mediated membrane fusion ». [Ames, Iowa : Iowa State University], 2008.
Trouver le texte intégralAbdulreda, Midhat H. « Investigation of Snare-Mediated Membrane Fusion Mechanism Using Atomic Force Microscope Spectroscopy ». Scholarly Repository, 2007. http://scholarlyrepository.miami.edu/oa_dissertations/55.
Texte intégralWebb, Stacy. « Viral Fusion Protein TM-TM Interactions : Modulators of Protein Function and Potential Antiviral Targets ». UKnowledge, 2017. http://uknowledge.uky.edu/biochem_etds/30.
Texte intégralKuwana, Tomomi. « Characterisation of a lysosomal protein that interfrers with membrane fusion assays ». Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337875.
Texte intégralMartinović, Vladan. « Protein complex formation and membrane remodelling in clathrin-mediated endocytosis ». Thesis, University of Cambridge, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.709269.
Texte intégralMühlenbrock, Peter [Verfasser]. « SNARE-mediated membrane fusion on pore-spanning membranes – several fusion pathways analyzed by single-vesicle content release / Peter Mühlenbrock ». Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2021. http://d-nb.info/1225121612/34.
Texte intégralHoffman, Mary M. « Mechanism of MDR protein mediated multidrug resistance / ». Access full-text from WCMC, 1997. http://proquest.umi.com/pqdweb?did=733008491&sid=6&Fmt=2&clientId=8424&RQT=309&VName=PQD.
Texte intégralDarsow, Tamara. « Molecular mechanisms for protein transport and regulation of membrane fusion in the vacuolar protein sorting pathway / ». Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2000. http://wwwlib.umi.com/cr/ucsd/fullcit?p3023449.
Texte intégralKuhlmann, Jan Wilhelm. « Modulation of lateral membrane tension and SNARE-mediated single vesicle fusion on pore spanning membranes ». Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2017. http://hdl.handle.net/11858/00-1735-0000-0023-3F13-E.
Texte intégralSuntoke, Tara R. « HIV entry : a biophysical and mutational analysis of gp41-mediated membrane fusion and its inhibition ». Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/31181.
Texte intégralIncludes bibliographical references.
The experiments described in this thesis were designed to elucidate the manner in which the HIV-1 envelope protein (Env) initiates infection of host cells, and to develop inhibitors of viral entry. Env comprises two non-covalently attached subunits, gpl20 and gp41, that associate as a trimer on the virion surface. Once gp 120 contacts the target cell, gp41 undergoes extensive conformational changes to mediate fusion of viral and cellular membranes. First, a short hydrophobic stretch of residues at the gp4 1 N-terminus insert into the target membrane, anchoring the protein in both viral and cellular membranes. This 'prehairpin' intermediate structure exposes an N-terminal a-helical coiled coil that is the target of promising antiviral peptides and small molecules. A previously unstudied region of N-terminal trimer was stabilized by fusion to a trimeric scaffold peptide and biophysically characterized (Chapter 2). This hybrid peptide itself potently inhibited HIV fusion, and the basis for this inhibition was assessed by studying mutant molecules. Efforts to use this peptide as an immunogen to elicit anti-gp41 antibodies are also outlined. Similar design strategies may be useful in developing N-terminal peptide inhibitors and HIV vaccine candidates, and in screening for antiviral molecules that bind to this region. As fusion progresses, the prehairpin intermediate resolves into a hairpin structure. This critical transition involves interaction of the N-terminal coiled coil with the gp41 C-terminal region. Evidence suggests that this N-C association provides the energy necessary to promote juxtaposition and merging of viral and cellular membranes.
(cont.) This hypothesis was tested using a biophysical and cell biological approach (Chapter 3), in which residues essential for this transition were mutated and analyzed. These studies confirm the hypothesis and highlight the importance of specific hydrophobic and polar contacts between the N- and C- terminal gp41 regions. This work contributes to a detailed understanding of the gp41 fusion machinery; furthermore, it shows that such knowledge can be used to design effective viral entry inhibitors. Finally, Chapter 4 places this work in the context of a broad overview of current drug and vaccine developments, and addresses some of the significant challenges that confront HIV researchers.
by Tara R. Suntoke.
Ph.D.
White, Paul. « Bacterial protein import mediated by an iron transporter ». Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:20298bb4-0998-4dad-9dfa-dd9e52854dec.
Texte intégralTroke, Philip J. F. « The leukaemogenic fusion protein MOZ-TIF2 inhibits nuclear receptor-mediated transcription and mislocalises CBP ». Thesis, University of Leicester, 2003. http://hdl.handle.net/2381/29687.
Texte intégralSchwamborn, Miriam. « Establishment of a fluorescence assay for characterization of protein-mediated vesicle fusion and acidification ». Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2017. http://hdl.handle.net/11858/00-1735-0000-0023-3E83-7.
Texte intégralFass, Deborah 1970. « The protein structures underlying receptor binding and membrane fusion of ecotropic murine leukemia viruses ». Thesis, Massachusetts Institute of Technology, 1997. http://hdl.handle.net/1721.1/10385.
Texte intégralSalsman, Scott J. « Redox regulation of protein tyrosine phosphatases in cell membrane receptor-mediated signal transduction ». Oklahoma City : [s.n.], 2005.
Trouver le texte intégralWitkowska, Agata [Verfasser], Reinhard [Akademischer Betreuer] Jahn, Reinhard [Gutachter] Jahn et Andreas [Gutachter] Janshoff. « Study of SNARE-mediated membrane fusion with a novel single vesicle fusion assay / Agata Witkowska ; Gutachter : Reinhard Jahn, Andreas Janshoff ; Betreuer : Reinhard Jahn ». Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2018. http://d-nb.info/1161183191/34.
Texte intégralRyan, Marnie A. « The Role of Membrane Remodeling in Surfactant Protein B (SP-B) Function ». University of Cincinnati / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1127263783.
Texte intégralJoshi, Supriya. « Role of membrane fusion protein Ykt6 in regulating epithelial cell-cell and cell-matrix adhesions ». VCU Scholars Compass, 2014. http://scholarscompass.vcu.edu/etd/3350.
Texte intégralLing, Rebecca. « Construction of a fusion protein for anchoring the inflammatory receptor NLRP3 to the cell membrane ». Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17482.
Texte intégralBokvist, Marcus. « Membrane mediated aggregation of amyloid-β protein : a potential key event in Alzheimer's disease ». Doctoral thesis, Umeå universitet, Kemi, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-969.
Texte intégralHuber, Scott David. « On Protein Recruitment Dynamics in Clathrin-Mediated Endocytosis and its Relation to Membrane Tension ». The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1545921340769193.
Texte intégralWilliamson, Shawn T. « Trafficking of integral membrane proteins of the inner nuclear membrane can be mediated by the ''sorting motif'' of autographa californica nucleopolyhedrovirus odv-e66 ». Texas A&M University, 2005. http://hdl.handle.net/1969.1/4353.
Texte intégralWasiak, Sylwia. « Characterization of protein-protein interactions involved in clathrin-mediated budding at the plasma membrane and the trans-Golgi network ». Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=85656.
Texte intégralFirst, we discovered that the endocytic protein PACSIN 1 is a binding partner for the signaling molecule mSos1. Further analysis of this interaction revealed that both proteins form a complex in vivo, that they co-localize at actin-rich sites, and that their interaction is regulated by phosphorylation. These data strengthen the link between endocytosis, signaling and actin cytoskeleton dynamics and provide the basis for further investigation of the role of this protein complex.
Second, we used subcellular proteomics to identify novel components of brain-derived CCVs. Among these was an ENTH domain-containing protein, which we named enthoprotin. Further work demonstrated that enthoprotin localizes to CCVs, interacts with clathrin adaptors AP-1 and GGA2 at the TGN, and stimulates clathrin assembly in vitro. Altogether, our data suggest a role for enthoprotin in clathrin coat assembly on internal membranes. Analysis of the enthoprotin protein sequence led to the discovery of two peptide motifs responsible for interactions with AP-1 and GGA2. We used alanine-scan mutagenesis and nuclear magnetic resonance to biochemically and structurally characterize the high-affinity site responsible for the interaction between enthoprotin and the TGN adaptors. This experimental approach, coupled to alignments, allowed us to deduce an AP-1/GGA-binding consensus motif that can be used towards the identification of novel TGN adaptor-binding partners. Altogether, my doctoral research contributed to furthering knowledge of the mechanisms that underlie clathrin-mediated membrane budding.
Chang, Andres. « EARLY EVENTS OF HUMAN METAPNEUMOVIRUS INFECTION ». UKnowledge, 2012. http://uknowledge.uky.edu/biochem_etds/5.
Texte intégralHuyton, Trevor. « Structural studies of p97, A AAA ATPase involved in membrane fusion and ubiquitin dependent protein pathways ». Thesis, Imperial College London, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.414829.
Texte intégralHaward, Fiona. « Investigation of the physiological roles of SRSF1-mediated translation ». Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/31188.
Texte intégralKalli, Antreas C. « Computational studies of talin-mediated integrin activation ». Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:ed4652dd-af20-4550-8c45-2b5f9c443ff6.
Texte intégralWan, Jun. « Elucidation of the JNK pathway mediated by Epstein-Barr virus encoded latent membrane protein 1 / ». View abstract or full-text, 2004. http://library.ust.hk/cgi/db/thesis.pl?BICH%202004%20WAN.
Texte intégralIncludes bibliographical references (leaves 105-128). Also available in electronic version. Access restricted to campus users.
Wang, Mingyang [Verfasser]. « Attachment of stearic acid to the Hemagglutinin-Esterase-Fusion (HEF) protein of influenza C virus affects membrane fusion and virus replication / Mingyang Wang ». Berlin : Freie Universität Berlin, 2015. http://d-nb.info/1077211996/34.
Texte intégralNour, El Din Suzanne [Verfasser]. « Azurin (P28) fusion protein mediated photodynamic therapy in the treatment of malignant tumors / Suzanne Nour El Din ». Ulm : Universität Ulm, 2017. http://d-nb.info/1140118137/34.
Texte intégralWu, Liming. « Elucidation of the NF-kB pathway mediated by Epstein - Barr virus-encoded latent membrane protein 1 (LMP1) / ». View abstract or full-text, 2005. http://library.ust.hk/cgi/db/thesis.pl?BICH%202005%20WU.
Texte intégralDashan, Li. « Factors affecting the membrane fusion-inducing capacity of the spike protein of avian infectious bronchitis coronavirus (IBV) ». Thesis, Royal Veterinary College (University of London), 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.522192.
Texte intégralTam, John. « Expression of the membrane fusion protein of measles virus in insect and mammalian cells using recombinant viruses ». Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=69521.
Texte intégralPostigo, Peláez Miguel Ángel. « Construction of a Fusion Gene : to anchor a truncated version of the inflammatory receptor NLRP3 to the cell membrane ». Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17573.
Texte intégralGaddy, Jennifer Angeline. « Acinetobacter baumannii Virulence Attributes : The Roles of Outer Membrane Protein A, Acinetobactin-mediated Iron Acquisition Functions, and Blue Light Sensing Protein A ». Miami University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=miami1289178632.
Texte intégralKlimyte, Edita M. « ELUCIDATING BINDING, FUSION AND ENTRY OF HUMAN METAPNEUMOVIRUS ». UKnowledge, 2016. http://uknowledge.uky.edu/biochem_etds/28.
Texte intégralHennerdal, Aron, Jenny Falk, Erik Lindahl et Arne Elofsson. « Internal duplications in alpha-helical membrane protein topologies are common but the nonduplicated forms are rare ». Stockholms universitet, Institutionen för biokemi och biofysik, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-51243.
Texte intégralauthorCount :4
Ding, Daniel N. « Membrane display of a fusion protein containing the ice-nucleation protein from Pseudomonas syringae and ScFv against c-myc oncoprotein in recombinant Escherichia coli ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape8/PQDD_0001/MQ42060.pdf.
Texte intégralBharadwaj, Prashant R. « Yeast as a Model for Studying Aβ Aggregation, Toxicity and Clearance ». Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2011. https://ro.ecu.edu.au/theses/404.
Texte intégralBöhm, Daniela [Verfasser]. « Investigation of the mechanism of Epstein-Barr virus Latent Membrane Protein 1 mediated NF-kB activation / Daniela Böhm ». Berlin : Freie Universität Berlin, 2010. http://d-nb.info/102450249X/34.
Texte intégralAndreazza, Camilla. « Mechanism of atlastin-mediated membrane fusion and identification of atlastin functional partners involved in endoplasmic reticulum dynamics using Drosophila as a model ». Doctoral thesis, Università degli studi di Padova, 2013. http://hdl.handle.net/11577/3423008.
Texte intégralRiassunto La biogenesi e il mantenimento del Reticolo Endoplasmatico (ER) richiedono fusione delle membrane. La fusione omotipica delle membrane dell'ER dipende dalla GTPasi atlastina, tuttvia i meccanismi che governano il processo di fusione mediato da questa proteina sono ancora sconosciuti. Attraverso un’analisi struttura-funzione, abbiamo esaminato i meccanismi alla base del processo di fusione nucleotide-dipendente di atlastina nell’organismo modello Drosophila melanogaster. L'analisi dei domini di atlastina mostra che una regione conservata nella coda citoplasmatica C-terminale è indispensabile per la sua attività fusogena. Atlastina priva del dominio C-terminale non permette la fusione delle membrane. L’espressione di atlastina wild-type di Drosophila causa alterazione del caratteristico fenotipo reticolare dell'ER e marcatori del Reticolo evidenziano dilatazioni delle membrane reticolari di varie dimensioni. Al contrario, l'espressione di atlastina tronca della regione C-terminale non altera la morfologia del ER dimostrando che questa forma troncata è poco o non funzionale in vivo. L'espressione di atlastina tronca ha permesso di identificare un dominio intermedio costituito da un fascio di tre eliche che sono importanti per l'oligomerizzazione di atlastina. Mutazioni che distruggono la struttura delle alfa-eliche di questo dominio inattivano atlastina prevenendo l'avvicinamento e la conseguente fusione delle membrane del Reticolo Endoplasmatico. Inoltre, l’aumento della distanza di formazione del complesso di atlastina dalle membrane destinate a fondersi inibisce la fusione, suggerendo che questa distanza è cruciale per atlastina per promuovere la fusione. Recentemente è stato visto che atlastina interagisce con altre proteine coinvolte nel determinare la morfologia dell'ER appartenenti alle famiglie reticulon e REEP/Yop/DP1. I nostri studi hanno dimostrato che Dp1 e reticulon interagiscono funzionalmente con atlastina. La letalità dovuta a mutazione nulla di atlastina è recuperata dalla simultanea perdita di funzione di reticulon. Inoltre, l fenotipo di iperfusione causato da sovraespressione di atlastina in cellule COS-7 è recuperato co-esprimendo Dp1 o reticulon. Questo risultato è supportato da esperimenti nell'occhio di Drosophila. Espressione di atlastina wild type nell'occhio di Drosophila causa un occhio piccolo e rovinato. La sovraespressione simultanea di Dp1 e atlastina nell'occhio risulta nel recupero del fenotipo causato dall'espressione di atlastina. Al contrario, l'assenza di reticulon aumenta il fenotipo di occhio piccolo e rovinato dovuto a espressione di atlastina. Questi risultati mostrano una forte interazione genetica antagonistica tra atlastina e reticulon o Dp1, suggerendo che questa proteine esercitano funzioni opposte per regolare la struttura dell'ER. Ipotizziamo che un bilanciamento tra le attività di atlastina, reticulon e Dp1 sia importante nel mantenimento e nel determinare la morfologia del Reticolo Endoplasmatico.
Baljinnyam, Bolormaa. « Untersuchungen zur F-proteinvermittelten Fusion von Paramyxoviren ». Doctoral thesis, [S.l.] : [s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=968791549.
Texte intégralOjelabi, Ogooluwa A. « Small Molecule Modulation of GLUT1-Mediated Glucose Transport ». eScholarship@UMMS, 2017. https://escholarship.umassmed.edu/gsbs_diss/950.
Texte intégralIshikawa, Raga. « Development of Engineered Extracellular Vesicle-Liposome Hybrid Using Baculovirus-Expression System ». Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/263686.
Texte intégral