Bibliographies thématiques / Protein material

Littérature scientifique sur le sujet « Protein material »

Auteur : Grafiati
Publié le 10 mars 2023

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Articles de revues sur le sujet "Protein material"

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Carter, Nathan A., et Tijana Z. Grove. « Functional protein materials : beyond elastomeric and structural proteins ». Polymer Chemistry 10, no 23 (2019) : 2952–59. http://dx.doi.org/10.1039/c9py00337a.

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In the past two decades researchers have shown great interest in mimicking biological structures and their complex structure–property relationships. Herein we highlight examples of hydrogels and bioelectronic materials that illustrate the rational design of material properties and function.
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Whyburn, Gordon P., Yujing Li et Yu Huang. « Protein and protein assembly based material structures ». Journal of Materials Chemistry 18, no 32 (2008) : 3755. http://dx.doi.org/10.1039/b807421f.

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Nichols, Patrick E., Jeffrey S. Bates et Taylor D. Sparks. « Exploration of Polytetrafluoroethylene as a Potential Material Replacement for Hemodialysis Applications ». MRS Advances 1, no 29 (2016) : 2147–53. http://dx.doi.org/10.1557/adv.2016.473.

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AbstractDialysis is the process by which an artificial kidney device removes waste and excess water from a patient. An outstanding problem with dialysis is that the body has a remarkable immune function where proteins and antigens mark foreign objects as possible threats despite the biocompatibility of the material. Upon adhesion to polymeric materials used currently in dialysis, proteins are lost. In this study, polytetrafluoroethylene (PTFE) is investigated as a potential replacement material for dialysis tubing because of its unreactive nature. The focus is to determine if PTFE will prove a viable material in minimizing protein adhesion and further reducing antibody loss of the patient. Protein loss as a function of filtration time was measured. PVC and PTFE materials were investigated following the same battery of testing where the protein concentrations in the blood were characterized using UV Visible spectrophotometry. Results demonstrate a loss of nearly 12 percent of blood proteins to the PVC material over the course of a typical dialysis treatment. Conversely, the protein loss due to adhesion to PTFE was less than two percent.
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Jawerth, Louise, Elisabeth Fischer-Friedrich, Suropriya Saha, Jie Wang, Titus Franzmann, Xiaojie Zhang, Jenny Sachweh et al. « Protein condensates as aging Maxwell fluids ». Science 370, no 6522 (10 décembre 2020) : 1317–23. http://dx.doi.org/10.1126/science.aaw4951.

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Protein condensates are complex fluids that can change their material properties with time. However, an appropriate rheological description of these fluids remains missing. We characterize the time-dependent material properties of in vitro protein condensates using laser tweezer–based active and microbead-based passive rheology. For different proteins, the condensates behave at all ages as viscoelastic Maxwell fluids. Their viscosity strongly increases with age while their elastic modulus varies weakly. No significant differences in structure were seen by electron microscopy at early and late ages. We conclude that protein condensates can be soft glassy materials that we call Maxwell glasses with age-dependent material properties. We discuss possible advantages of glassy behavior for modulation of cellular biochemistry.
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Srinivasan, U., G. H. Iyer, T. A. Przybycien, W. A. Samsonoff et J. A. Bell. « Crystine : fibrous biomolecular material from protein crystals cross-linked in a specific geometry ». Protein Engineering, Design and Selection 15, no 11 (novembre 2002) : 895–902. http://dx.doi.org/10.1093/protein/15.11.895.

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Baskaran, Nareshkumar, You-Cheng Chang, Chia-Hua Chang, Shun-Kai Hung, Chuan-Tse Kao et Yang Wei. « Quantify the Protein–Protein Interaction Effects on Adsorption Related Lubricating Behaviors of α-Amylase on a Glass Surface ». Polymers 12, no 8 (25 juillet 2020) : 1658. http://dx.doi.org/10.3390/polym12081658.

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Dental ceramic material is one of the widely preferred restorative materials to mimic the natural tooth enamel surface. However, it has continuously been degraded because of low wear resistance during mastication in the oral cavity. The friction involved was reduced by introducing the lubricant saliva protein layers to improve the wear resistance of the dental materials. However, little is understood regarding how the protein–protein interactions (PPI) influence the adsorbed-state structures and lubricating behaviors of saliva proteins on the ceramic material surface. The objective of this study is to quantify the influences of PPI effects on the structural changes and corresponding oral lubrications of adsorbed α-amylase, one of the abundant proteins in the saliva, on the dental ceramic material with glass as a model surface. α-Amylase was first adsorbed to glass surface under varying protein solution concentrations to saturate the surface to vary the PPI effects over a wide range. The areal density of the adsorbed protein was measured as an indicator of the level of PPI effects within the layer, and these values were then correlated with the measurements of the adsorbed protein’s secondary structure and corresponding friction coefficient. The decreased friction coefficient value was an indicator of the lubricated surfaces with higher wear resistance. Our results indicate that PPI effects help stabilize the structure of α-amylase adsorbed on glass, and the correlation observed between the friction coefficient and the conformational state of adsorbed α-amylase was apparent. This study thus provides new molecular-level insights into how PPI influences the structure and lubricating behaviors of adsorbed protein, which is critical for the innovations of dental ceramic material designs with improved wear resistance.
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Zegers, Ingrid, Thomas Keller, Wiebke Schreiber, Joanna Sheldon, Riccardo Albertini, Søren Blirup-Jensen, Myron Johnson et al. « Characterization of the New Serum Protein Reference Material ERM-DA470k/IFCC : Value Assignment by Immunoassay ». Clinical Chemistry 56, no 12 (1 décembre 2010) : 1880–88. http://dx.doi.org/10.1373/clinchem.2010.148809.

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BACKGROUND The availability of a suitable matrix reference material is essential for standardization of the immunoassays used to measure serum proteins. The earlier serum protein reference material ERM-DA470 (previously called CRM470), certified in 1993, has led to a high degree of harmonization of the measurement results. A new serum protein material has now been prepared and its suitability in term of homogeneity and stability has been verified; after characterization, the material has been certified as ERM-DA470k/IFCC. METHODS We characterized the candidate reference material for 14 proteins by applying a protocol that is considered to be a reference measurement procedure, by use of optimized immunoassays. ERM-DA470 was used as a calibrant. RESULTS For 12 proteins [α2 macroglobulin (A2M), α1 acid glycoprotein (orosomucoid, AAG), α1 antitrypsin (α1-protease inhibitor, AAT), albumin (ALB), complement 3c (C3c), complement 4 (C4), haptoglobin (HPT), IgA, IgG, IgM, transferrin (TRF), and transthyretin (TTR)], the results allowed assignment of certified values in ERM-DA470k/IFCC. For CRP, we observed a bias between the lyophilized and liquid frozen materials, and for CER, the distribution of values was too broad. Therefore, these 2 proteins were not certified in the ERM-DA470k/IFCC. Different value transfer procedures were tested (open and closed procedures) and found to provide equivalent results. CONCLUSIONS A new serum protein reference material has been produced, and values have been successfully assigned for 12 proteins.
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Ganesan, Ramakrishnan, Karl Kratz et Andreas Lendlein. « Multicomponent protein patterning of material surfaces ». Journal of Materials Chemistry 20, no 35 (2010) : 7322. http://dx.doi.org/10.1039/b926690a.

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Messia, Maria Cristina, Francesca Cuomo, Luisa Falasca, Maria Carmela Trivisonno, Elisa De Arcangelis et Emanuele Marconi. « Nutritional and Technological Quality of High Protein Pasta ». Foods 10, no 3 (11 mars 2021) : 589. http://dx.doi.org/10.3390/foods10030589.

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Pasta has an important role in human nutrition for its high content of complex carbohydrates and its widespread use. It can be an efficient delivery system or carrier of non-traditional raw material, including additional health-promoting ingredients. The partial replacement of semolina with high-protein raw materials leads to the improvement of the biological value of pasta proteins. In order to obtain pasta with high nutritional protein value and with excellent cooking properties, various recipes have been formulated with different percentages of semolina and unconventional high-protein raw materials (peas and soy isolate proteins, egg white, whey proteins and Spirulina platensis). High-protein pasta was produced using a pasta making pilot plant and the nutritional quality (protein content and quality) and sensorial properties were assessed. All experimental pastas showed optimal performances. Pasta prepared with pea protein isolate, whey proteins and Spirulina platensis showed improved chemical score and digestible indispensable amino acid scores, an eye-catching color, and an excellent cooking quality.
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Woodruff, Jeffrey B., Oliver Wueseke et Anthony A. Hyman. « Pericentriolar material structure and dynamics ». Philosophical Transactions of the Royal Society B : Biological Sciences 369, no 1650 (5 septembre 2014) : 20130459. http://dx.doi.org/10.1098/rstb.2013.0459.

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A centrosome consists of two barrel-shaped centrioles embedded in a matrix of proteins known as the pericentriolar material (PCM). The PCM serves as a platform for protein complexes that regulate organelle trafficking, protein degradation and spindle assembly. Perhaps most important for cell division, the PCM concentrates tubulin and serves as the primary organizing centre for microtubules in metazoan somatic cells. Thus, similar to other well-described organelles, such as the nucleus and mitochondria, the cell has compartmentalized a multitude of vital biochemical reactions in the PCM. However, unlike these other organelles, the PCM is not membrane bound, but rather a dynamic collection of protein complexes and nucleic acids that constitute the organelle's interior and determine its boundary. How is the complex biochemical machinery necessary for the myriad centrosome functions concentrated and maintained in the PCM? Recent advances in proteomics and RNAi screening have unveiled most of the key PCM components and hinted at their molecular interactions ( table 1 ). Now we must understand how the interactions between these molecules contribute to the mesoscale organization and the assembly of the centrosome. Among outstanding questions are the intrinsic mechanisms that determine PCM shape and size, and how it functions as a biochemical reaction hub.
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Thèses sur le sujet "Protein material"

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Ilic, Natasa, Nektaria Lalangas, Jowan Rostami et Alexander Wiorek. « Nya material från protein-nanofibrer ». Thesis, KTH, Skolan för kemivetenskap (CHE), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-208749.

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Under det här kandidatexamensarbetet har protein-nanofibers påverkan på material undersökts genom att jämföra fibrillerade filmer med ofibrillerade. Sojaproteinisolat fibrillerades under förhållandena pH 2 och 85 ◦C under minst ett dygn och de syntetiserade nanofibrerna analyserades med Thioflavin T (ThT) fluorescens och atomkraftsmikroskopi (AFM). Spektra från analysmetoden ThT fluorescens indikerade på förekomsten av β-flak och analyserna med AFM visade på att fibrerna hade en morfologi som är karakteristisk för protein-nanofibrer. Resultaten antyder att de parametrar som påverkar morfologin hos fibrerna är fibrilleringstid och typ av protein. De gjutna filmerna från fibrillära respektive ofibrillära proteiner var sammanhängande bortsett från vissa sprickor. Värdena på E-modulen från AFM visade att det fibrillerade materialet var mer heterogent än det ofibrillerade. Filmer med sammanhängande yta erhölls vid tillsats av det mjukgörande additivet glycerol. Slutligen, material av både fibrillär och ofibrillär form kan framställas, däremot krävs vidare forskning för att optimera materialens egenskaper.
During this bachelor thesis project, the impact of protein nanofibers on materials has been analysed by comparing films made from fibrillar and non-fibrillar protein. Fibrillation of soy protein isolate was performed during at least 24 hours at pH 2 and a temperature of 85 ◦C. Analysis of the nanofibers was made with Thioflavin T (ThT) fluorescence and atomic force microscopy (AFM). The spectra from ThT Fluorescens indicated the presence of β-sheets and AFM confirmed that the fibrils had a morphology that is characteristic of protein nanofibers. The results indicated that heating time and protein type were the parameters which had the largest impact on the morphology of the fibrils. The synthesised films from both fibrillar and non-fibrillar protein were coherent with exception of some cracks. The elastic modulus from AFM indicated that the fibrillar film was more heterogeneous compared to the non-fibrillar film. To attain coherent films, the plasticising agent glycerol was added. To summarise, both fibrillar as well as non-fibrillar materials were successfully synthesised, however, further research is necessary to optimise the properties of the material.
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Rosengren, Åsa. « Cell-protein-material interactions on bioceramics and model surfaces / ». Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4688.

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Rosengren, Åsa. « Cell-protein-material Interactions on Bioceramics and Model Surfaces ». Doctoral thesis, Uppsala University, Surface Biotechnology, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4688.

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The objective of this thesis was to investigate and characterize the interaction between blood proteins and different surfaces with emphasis on protein adsorption to bioceramics and model surfaces. Special effort was made to monitor the spontaneous and selective adsorption of proteins from human plasma and to examine the orientation, conformation and functional behavior of single proteins after adsorption.

Five different ceramic biomaterials: alumina (Al2O3), zirconia (ZrO2), hydroxyapatite (Ca10(PO4)6(OH)2) and two glass-ceramics, AP40 (SiO2-CaO-Na2O-P2O5-MgO-K2O-CaF2) and RKKP (AP40 with Ta2O3-La2O3), were exposed to human plasma and their protein binding capacities and affinities for specific proteins were studied by chromatography, protein assays, two-dimensional gel electrophoresis and Western blotting. The studies showed that all materials adsorbed approximately the same high amount of plasma proteins and that they therefore should be fully covered by proteins in an in vivo setting. The adsorbed proteins were different for most materials which could explain their previously observed different levels of tissue integration in vivo.

Four of the proteins that behaved differently, ceruloplasmin, prothrombin, α2-HS-glycoprotein and α1-antichymotrypsin, were selected for characterization with atomic force microscopy and ellipsometry. The studies, which were performed on ultraflat silicon wafers (silica), showed that the proteins oriented themselves with their long axis parallel to the surface or as in case of ceruloplasmin with one of its larger sides towards the surface. All of them had globular shapes but other conformational details were not resolved. Furthermore, prothrombin (none of the others) formed multilayers at high proteins concentrations.

The functional behaviour of the adsorbed proteins, referring to their cell binding and cell spreading capacity on silica and a positive cell adhesion reference surface (Thermanox®), was affected by the underlying substrate. Ceruloplasmin, α2-HS-glycoprotein and α1-antichymotrypsin stimulated cell attachment to silica, but suppressed attachment to Thermanox®. Prothrombin stimulated cell attachment to both surfaces. The attachment was in most cases mediated both by cell membrane-receptors (integrins) and by non-specific interactions between the cell and the material.

This thesis showed that the compositional mixture, orientation, conformation and functional behavior of the adsorbed proteins are determined by the properties of the underlying surface and if these parameters are controlled very different cellular responses can be induced.

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González, García Cristina. « BIOLOGICAL ACTIVITY OF FIBRONECTIN AT THE CELL-MATERIAL INTERFACE ». Doctoral thesis, Universitat Politècnica de València, 2012. http://hdl.handle.net/10251/17701.

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Esta tesis aborda la actividad biológica de la fibronectina (FN) como proteína de interfase en la interacción célula-material. La tesis investiga la respuesta de la proteína, en términos de cantidad adsorbida y conformación, ante diferentes propiedades físico-químicas del material. Además, se correlaciona la respuesta celular temprana y la funcionalidad celular con el estado de la proteína adsorbida sobre el material. Para ello se prepararon diferentes series de materiales con propiedades físico-químicas controladas. La distribución de FN sobre las diferentes superficies se caracterizó mediante el uso de la microscopía de fuerza atómica (AFM) y la densidad superficial adsorbida fue cuantificada mediante técnicas de marcado radioactivo y western blot. La respuesta celular se evaluó en términos de la adhesión inicial a las superficies, así como los procesos posteriores de diferenciación, proliferación, reorganización y producción de matriz extracelular. Se investigó el efecto de la nanotopografía en la adsorción de la FN y el comportamiento celular sobre una serie de topografías controladas en la escala nanométrica, obtenidas mediante el spin casting de soluciones de ácido poli(L-láctico)/poliestireno (PLLA/PS) de distintas concentraciones. La migración del PLLA hacia la superficie del film durante el proceso de spin coating proporciona superficies de PLLA con nanopicos de diferentes tamaños (14, 29 y 45 nm). El tamaño de la nanoestrutura afecta a la densidad de FN adsorbida, siendo mayor en la superficie de menor nanotopografía. En cuanto a la respuesta celular inicial, se observan adhesiones focales más desarrolladas y mejor reorganización celular de la capa de FN adsorbida en las superficies de mayor topografía (29 and 45 nm), lo que resulta en una mayor producción y organización de nueva matriz. Por otra parte se empleó una familia de materiales con sutiles variaciones en la composición química: polímeros acrílicos (polimetil, etil y butil acrilato -PMA, PEA y P
González García, C. (2012). BIOLOGICAL ACTIVITY OF FIBRONECTIN AT THE CELL-MATERIAL INTERFACE [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/17701
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Turnbull, Robert Edward. « Regulation of monocyte anti-thrombotic gene and protein expression through platelet-derived material ». Thesis, University of Leicester, 2016. http://hdl.handle.net/2381/36700.

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Activated platelets can recruit monocytes into thrombi through the P-selectin*PSGL-1 interaction. Cross-talk between the cells may regulate monocyte gene and protein expression. A previous genome-wide transcriptomic study identified >3000 genes upregulated in monocytes in response to activated platelets, including a number of antithrombotic genes; in particular tfpi and procr. This thesis aimed to establish the mechanisms by which platelets regulate these genes, and if this was through the nuclear receptor PPARγ. Using the same experimental model as in previous studies, in which platelets were activated in whole blood with the platelet-specific GPVI agonist CRP-XL, monocyte tfpi and procr expression was confirmed, shown to be affected by inhibitors of COX-1 (aspirin) and 12-LOX (esculetin and baicalein), and regulated through soluble factors released from platelets, which were shown to be oxylipins for tfpi and proteins ~10kDa for procr. Expression of pparγ was also increased and largely regulated through direct platelet-monocyte contact, although oxylipins potentiated expression. An associated increase in protein expression was partially confirmed for all three genes. Mass spectrometry (MS) of platelet-derived releasate measured 386 proteins and identified platelet factor 4 (PF4) and RANTES (CCL5) as likely candidates for procr regulation. Expression was attenuated with releasate incubated with heparin agarose (HA) but this was not rescued with exogenous PF4. LC-MS/MS measured no change in PF4 levels in the releasate incubated with HA but complete removal of RANTES. MS identified 10 oxylipins released from platelets (AA, 8-, 9-, 11-, 12-, 15-HETE, 9-, 13-HODE, PGD/E2 and TxA2) of which four (11-, 15-HETE, 9-, 13-HODE) were significantly, and TxA2 and PGD/E2 completely reduced by aspirin, and all but AA and 9-HETE reduced with 12-LOX inhibitors. These oxylipins included known PPARγ agonists. Incubation with 15d-PGJ2 and rosiglitazone confirmed potentiation of tfpi expression in monocytes. Using a transactivation assay 12- and 15-HETE were shown to activate PPARγ expression in vitro, while X-ray crystallography indicated, for the first time, interaction of both with PPARγ. These results are the first to show regulation of antithrombotic genes in monocytes by factors released from platelets. It is the first to profile oxylipins released from GPVI-activated platelets and identify PPARγ as a regulator of tfpi, and RANTES as a potential regulator of procr expression in monocytes. The observation that aspirin attenuates expression of both these genes raises issues with its use in the treatment of cardiovascular disease, for which it is relied on heavily.
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Allen, Mark Andrew. « Protein Cage Architectures as a Nano-Platform for Material Synthesis and Metal Binding ». Thesis, Montana State University, 2006. http://etd.lib.montana.edu/etd/2006/allen/AllenM0806.pdf.

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Supramolecular proteins that assemble into cage like architectures have been used for nano-material synthesis and as a scaffold for metal binding. Material synthesis can be performed by exploiting the cage-like properties of these nano-containers and relying on the electrostatically distinct interior environment that drive mineral encapsulation. Ferritin and ferritin like proteins can be used as size constrained reaction vessels that encapsulate materials that have sizes that are determined by the internal dimensions of the protein cage. These range from 5 nm for the ferritin like protein from Listeria innocua to 24 nm for the interior of an engineered plant virus (Cowpea chlorotic mottle virus). Inorganic materials synthesized within these constrained reaction volumes are monodisperse in size. The crystallinity and phase of material prepared is determined by the reaction conditions, which are mild compared to other preparative methods. The metal binding affinity of certain viral protein cages allows the study of the role that metals play in such processes as viral assembly and infection as well as transmission. If paramagnetic metal ions are bound to the viral protein cage, the biological scaffold has potential use as an MRI contrast agent. Here multiple protein cage platforms are discussed with an emphasis on engineering non-native functionality to many of the protein cages. Engineering nonnative function to protein cages involves both genetic and chemical modification for the purpose of increasing stability and changing electrostatic interactions. Together these modifications serve to reinforce the versatility of protein cage architectures for both mineral synthesis and metal binding.
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Llopis, Hernández Virginia. « Material-driven fibronectin fibrillogenesis to engineer cell function ». Doctoral thesis, Universitat Politècnica de València, 2017. http://hdl.handle.net/10251/90412.

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This thesis ventures with the extracellular matrix protein (ECM) fibronectin (FN) as an interface protein in the interaction between cells and materials to design microenvironment for future use in tissue engineering. It is studied the FN adsorption and conformations, cell behaviour to different FN conformation, cell adhesion, reorganisation and remodelling of FN at the material interface, the role of growth factors (GF) and their interactions with components of the extracellular matrix (ECM), the immunology cell response, and the stem cell fate influenced by the extrinsic signals coming from the engineered microenvironments using ECM's proteins. To investigate the FN response, in terms of adsorbed amount and conformation to different chemical properties of the material, model surfaces were used. Self assembled monolayers (SAM) with different percentages of two different chemical groups were used: CH3 and OH. FN adsorption, initial cell adhesion and signalling (focal adhesions, integrin expression and phosphorylation of FAK) is related with the reorganisation and secretion of FN and matrix degradation. It is shown that matrix degradation at the cell material interface depends on surface chemistry in metalloproteinase-dependent way. A direct relationship between FN activity at the cell-material interface and metalloproteinase 9 (MMP9) expression was found, being the product of a sequence of events that include integrin expression, focal adhesion formation, matrix reorganisation and focal adhesion kinase (FAK) phosphorylation. Two different materials with subtle variations in their chemical composition were employed as a drastically different FN conformation: from a globular conformation on PMA (poly (methyl acrylate)) to the formation of a well-interconnected FN network (similar to the FN physiological fibrillar network) triggered by PEA (poly (ethyl acrylate)). The formation of focal adhesions (vinculin), FAK expression and phosphorylation, specific integrin binding, protein and gene expression for ¿5 and ¿v was studied, seeking to correlate cell adhesion with matrix degradation. It is demonstrated that the material-driven FN fibrillogenesis on PEA triggers proteolytic activity: MMP activity is higher as a compensatory mechanism to the inability of cells to reorganise this FN network. Looking into the role of protein-material interactions and stem cell fate, and with the knowledge on PEA, we engineer different synergistic microenvironments to direct cell and stem cell fate. FN has a growth factor (GF) binding domain on its molecule (FNIII12-14) and has been demonstrated to produce a synergistic response when occurs at the same time the recognition of the cell binding domain (FNIII9-10). It is demonstrated that this domain is available on the FN coated PEA, and exploiting these interactions between PEA, FN and GF, it is developed a microenvironment to control cell behaviour and tissue repair. It is studied the BMP2 binding and presentation, the effect of BMP2 presentation on MSC proliferation and differentiation. These systems allow not only enhanced activity of GF compared to soluble administration, but also reduce GF doses, improving safety and cost effectiveness. Finally, the immunological reaction of the microenvironment developed is studied using dendritic cells, beside the conformational structure of ECM protein importance in DC integrin-based activation it is studied, helping to establish the field of adhesion-based modulation of DC as a general mechanism that has previously not been defined. The microenvironment didn't induce any maturation in DC, while different FN conformation shows differences in DC morphology and citokine level production (IL-10 and IL-12).
En esta tesis se estudia la interacción de una proteina de la matriz extracelular, fibronectina (FN) como interfase en la interacción entre células y materiales, para diseñar microambientes con el propósito de ser usados en el futuro en ingeniería tisular. Se estudia la adsorción y conformación de FN y la relación con el diferente comportamiento celular: la adhesión celular, la reorganización y remodelado de la FN en la interfase célula-material, el papel que juegan los factores de crecimiento y sus interacciones con los componentes de la matriz extracelular, la respuesta immunológica y el destino celular de células madre influenciadas por las señales extrínsecas provenientes de microambientes elaborados a partir de proteínas de la matriz extracelular. Con el objetivo de investigar la respuesta a la FN en términos de conformación y cantidad absorbida a diferentes propiedades químicas del material, se usaron materiales modelo: monocapas autoensambladas (self-assembled monolayers, SAM). Las químicas estudiadas fueron CH3 and OH. La adsorption de FN, adhesion y señalización (adhesiones focales, expresión de interinas y fosforilación de quinasas de adhesiones focales (FAK)) se estudiaron en relación a la reorganización y secreción de FN y degradación de la matriz extracelular. Se demuestra que la degradación de la matriz extracelular en la interfase célula-material depende de la química de la superficie, a través de las metaloproteinasas. Se ha descubierto una relación directa entre la actividad de la FN que se encuentra en el material y la expresión de metaloproteinasa 9 (MMP9), a través de la expresión de integrinas, formación de adhesiones focales, reorganización de la matriz extracelular y fosforilación de FAK En el siguiente capítulo se emplean materiales poliméricos con una sutil diferencia en la composición química, provocando una diferencia drástica en la conformación de la FN: se pasa de una conformación globular en PMA (polimetil acrilato) a una conformación en forma de red interconectada en PEA (polietil acrilato). Con el propósito de relacionar la adhesión celular con la degradación de la matriz extracelular, se estudia la formación de adhesiones focales (vinculina), la expresión y fosforilación de FAK, la unión específica de integrinas y la expresión de las integrinas ¿5 and ¿v. Se demuestra que la formación de una red de FN sobre PEA induce la actividad proteolítica: la actividad de las MMPs es mayor, actuando como mecanismo compensatorio a la incapacidad de reorganización de la red de FN. Haciendo uso de la conformación de la FN sobre PEA, se estudiaron las interacciones entre la proteína-material y el destino celular de células madres. La FN posee un dominio de unión de factores de crecimiento (FNIII12-14) y se ha demostrado que se produce una respuesta sinérgica cuando el reconocimiento ocurre junto con el dominio de unión celular (FNIII9-10). En esta tesis se demuestra que el dominio de unión de factores de crecimiento está disponible en la conformación que adquiere sobre PEA y se diseñan microambientes para controlar el comportamiento celular y regeneración de tejido. Se estudia la unión y presentación de BMP2 y su efecto en la diferenciación de células madre mesenquimales. Los microambientes desarrollados, ademas de mejorar la actividad de los factores de crecimiento comparado con la administración soluble, también reduce la cantidad de factores de crecimiento que se tendría que administrar, mejorando la seguridad y efectividad. Finalmente se estudió la reacción inmunológica a los microambientes desarrollados usando células dendríticas, estudiando además la influencia de la estructura de la conformación de las proteínas en la activación de las células dendríticas a través de las integrinas. Los microambientes no indujeron ninguna maduración de células dendríticas, mientras que la conformación de la FN muestra control
En aquesta tesi s'estudia la interacció entre una proteïna de la matriu extracel.lular, fibronectina (FN) com interfase en la interaccio entre cèl·lules i materials, per a dissenyar microambients amb el propòsit d'utilitzar-se al futur en enginyeria tissular. S'estudia l'adsorció i conformació de la FN i la relació amb el diferent comportament cel·lular: l'adhesió cel·lular, la reorganització i remodelat de la FN a la interfase cèl·lula-material, el paper que juguen els factors de creixement i les seus interaccions amb els components de la matriu extracel·lular, la resposta immunològica i el destí cel·lular de cèl·lules mare influenciades pels senyals extrínseques provinents de microambients elaborats a partir de proteïnes de la matriu extracel·lular. Amb l'objectiu d'investigar la respostar a la FN en termes de conformació i quantitat absorbida a diferents propietats químiques del material, s'utilitzaren materials model: monocapes autoacoblades (self-assembled monolayers, SAM). Les químiques estudiades van ser CH3 and OH. L'absorció de FN, adhesió i senyalització (adhesions focals, expressió d'integrines i fosforilació de quinases d'adhesions focals (FAK)) es van estudiar en relació a al reorganització i secreció de la FN i degradació de la matriu extracel·lular. Es demostra que la degradació de la matriu extracelular en la interfase cèl·lula-material depèn de la química de la superficie, a través de les metal·loproteïnases. S'ha descobert una relació directa entra l'activitat de la FN que es troba en el material i l'expressió de metaloproteinasa 9, a través de l'expressió d'integrines, formació d'adhesions focals, reorganització de la matriu extracel·lular i fosforilació de FAK. Al següent capítol es fan servir materials polimèrics amb una subtil diferència en la composició química, provocant una diferència dràstica en la conformació de la FN: es passa d'una conformació globular en PMA (polimetil acrilat) a una conformació en forma de xarxa interconnectada en PEA (polietil acrilat). Amb el propòsit de relacionar l'adhesió cel·lular amb la degradació de la matriu extracel·lular, s'estudia la formació d'adhesions focals (vinculina), l'expressió i fosforilació de FAK, la unió específica d'integrines i l'expressió de les integrines ¿5 and ¿v. Es demostra que la formació d'una xarxa de FN sobre PEA indueix l'activitat proteolítica: l'activitat de les MMPs és més gran, actuant com a mecanisme compensatori a la incapacitat de reorganització de la xarxa de FN. Fent ús de la conformació de la FN sobre PEA, es van estudiar les interaccions entre la proteïna-material i el destí cel·lular de cèl·lules mares. La FN posseeix un domini d'unió de factors de creixement (FNIII12-14) i s'ha demostrat que es produeix una resposta sinèrgica quan el reconeixement ocurreix juntament amb el domini d'unió cel·lular (FNIII9- 10). En aquesta tesi es demostra que el domini d'unió de factors de creixement està disponible a la conformació que adquireix sobre PEA i es dissenyen microambients per controlar el comportament cel·lular i regeneració de teixit. S'estudia la unió i presentació de BMP2 i el seu efecte en la diferenciació de cèl·lules mare mesenquimals. Els microambientes desenvolupats, a més de millorar l'activitat dels factors de creixement comparat amb l'administració soluble, també redueix la quantitat de factors de creixement que s'hauria d'administrar, millorant la seguretat i efectivitat. Finalment es va estudiar la reacció immunològica als microambients desenvolupats usant cèl·lules dendrítiques, estudiant a més la influència de l'estructura de la conformació de les proteïnes en l'activació de les cèl·lules dendrítiques a través de les integrines. Els microambients no van induir cap maduració de cèl·lules dendrítiques, mentre que la conformació de la FN mostra controlar la morfologia de les cèl·lules dendrítiques i
Llopis Hernández, V. (2017). Material-driven fibronectin fibrillogenesis to engineer cell function [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/90412
TESIS
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Bodlund, Ida. « Coagulant Protein from plant materials : Potential Water Treatment Agent ». Licentiate thesis, KTH, Industriell bioteknologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-107335.

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Access to fresh water is a human right, yet more than 780 million people, especially in rural areas, rely on unimproved sources and the need for finding ways of treating water is crucial. Although the use of natural coagulant protein in drinking water treatment has been discussed for a long time, the method is still not in practice, probably due to availability of material and limited knowledge. In this study, about hundred different crude extracts made from plant materials found in Southern India were screened for coagulation activity. Extracts of three Brassica species (Mustard, Cabbage and Cauliflower) were showing activity comparable to that of Moringa oleifera and were further investigated. Their protein content and profile were compared against each other and with coagulant protein from Moringa. Mustard (large) and Moringa seed proteins were also studied for their effect against clinically isolated bacterial strains. The protein profiles of Brassica extract showed predominant bands around 9kDa and 6.5kDa by SDS-PAGE. The peptide sequence analysis of Mustard large identified the 6.5kDa protein as Moringa coagulant protein (MO2.1) and the 9kDa protein band as seed storage protein napin3. Of thirteen clinical strains analysed, Moringa and Mustard large were proven effective in either aggregation activity or growth kinetic method or both in all thirteen and nine strains respectively. To my knowledge this is the first report on the presence of coagulant protein in Brassica seeds. Owing to the promising results Brassica species could possibly be used as a substitute to Moringa coagulating agent and chemicals in drinking water treatment.

QC 20121214

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Kappiyoor, Ravi. « Mechanical Properties of Elastomeric Proteins ». Diss., Virginia Tech, 2014. http://hdl.handle.net/10919/54563.

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When we stretch and contract a rubber band a hundred times, we expect the rubber band to fail. Yet our heart stretches and contracts the same amount every two minutes, and does not fail. Why is that? What causes the significantly higher elasticity of certain molecules and the rigidity of others? Equally importantly, can we use this information to design materials for precise mechanical tasks? It is the aim of this dissertation to illuminate key aspects of the answer to these questions, while detailing the work that remains to be done. In this dissertation, particular emphasis is placed on the nanoscale properties of elastomeric proteins. By better understanding the fundamental characteristics of these proteins at the nanoscale, we can better design synthetic rubbers to provide the same desired mechanical properties.
Ph. D.
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Robbins, Steven C. « Distribution of Colloidal Material in Activated Sludge as Influenced by Cations ». Thesis, Virginia Tech, 2004. http://hdl.handle.net/10919/45540.

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Activated sludge influent and effluent from five municipal wastewater treatment plants were analyzed to further elucidate the roles of aluminum, iron, and the monovalent to divalent cation ratio (M/D) on the influent and effluent characteristics of the systems. The size distribution of organic nitrogen, organic carbon, protein, humic acid, and polysaccharide was examined with respect to the concentration of cations in the activated sludge influent. It was found that the majority of organic nitrogen, organic carbon, protein and polysaccharides were found in material larger than 0.45μm in activated sludge influent. Humic acids were mostly found in material smaller than 0.45μm. Protein was the largest contributor to organic nitrogen and humic acids were the largest contributor to organic carbon. Using 0.45μm as a division between particulate and soluble material, the ratio of soluble to particulate material in activated sludge influent was found to be negatively correlated with the ratio of iron to aluminum. In activated sludge effluent, the majority of the organic nitrogen and protein was found in material larger than 0.45μm, while the majority of the organic carbon, humic acid, and polysaccharide were found in material smaller than 30kDa. Influent aluminum concentration had no observable effect on the concentration or distribution of organic nitrogen or organic carbon. Influent iron appeared to play a role in the flocculation of organic nitrogen and protein containing material between 0.45μm and 1kDa in size. A high monovalent to divalent cation ratio appeared to play a role in deflocculating organic nitrogen containing material larger than 1.5μm and increased the concentration of TOC smaller than 1kDa and the total polysaccharide concentration. Tertiary depth filtration removed all organic nitrogen and protein in material larger than 0.45μm, but a poor job of removing organic carbon from and an inconsistent job of removing polysaccharide from effluent Eight lab-scale activated sludge reactors were also run, but the data was not consistent enough for analysis and comparison with the municipal wastewater treatment plants. This thesis contains a series of four papers that each deal with a different aspect of the role of cations on activated sludge influent and effluent. The first paper focuses on activated sludge influent characteristics, the second on effluent organic nitrogen and organic carbon, the third on effluent EPS, and the last on the lab-scale reactors. The papers were divided in this way because of the unique analytical obstacles that were encountered with each set of data.
Master of Science
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Livres sur le sujet "Protein material"

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György, Marko-Varga, et Oroszlan Peter, dir. Emerging technologies in protein and genomic material anaylsis. Amsterdam : Elsevier, 2003.

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McGrath, Kevin, et David Kaplan, dir. Protein-Based Materials. Boston, MA : Birkhäuser Boston, 1997. http://dx.doi.org/10.1007/978-1-4612-4094-5.

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1962-, McGrath Kevin, et Kaplan David 1953-, dir. Protein-based materials. Boston : Birkhäuser, 1997.

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Program, Massachusetts Genetics. Maternal serum alpha-feto protein : Screening test. Boston, MA : Massachusetts Genetics Program, Division of Perinatal Health, Bureau of Parent, Child and Adolescent Health, Massachusetts Dept. of Public Health, 1990.

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Khataee, A. R. Mechanical and dynamical principles of protein nanomotors : The key to nano-engineering applications. New York : Nova Science Publishers, 2010.

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Khataee, A. R. Mechanical and dynamical principles of protein nanomotors : The key to nano-engineering applications. Hauppauge, N.Y : Nova Science Publishers, 2009.

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Dickmann, Nancy. Meat and protein. Chicago : Heinemann Library, 2011.

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Dickmann, Nancy. Meat and protein. Chicago : Heinemann Library, 2011.

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Seliger, Larsen Barbara, et McEwen Charles N. 1942-, dir. Mass spectrometry of biological materials. 2e éd. New York : Marcel Dekker, 1998.

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Stanisław, Błażewicz, dir. Biopolymers : Lignin, proteins, bioactive nanocomposites. Berlin : Springer, 2010.

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Chapitres de livres sur le sujet "Protein material"

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McKenzie, Janice L., Thomas J. Webster et J. L. McKenzie. « Protein Interactions at Material Surfaces ». Dans Biomedical Materials, 399–422. Cham : Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-49206-9_12.

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McKenzie, Janice L., et Thomas J. Webster. « Protein Interactions at Material Surfaces ». Dans Biomedical Materials, 215–37. Boston, MA : Springer US, 2009. http://dx.doi.org/10.1007/978-0-387-84872-3_8.

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Bragg, Jason G., et Andreas Wagner. « The Evolution of Protein Material Costs ». Dans Evolutionary Genomics and Systems Biology, 203–11. Hoboken, NJ, USA : John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470570418.ch11.

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Murtaza, Mian Anjum, et Kashif Ameer. « Food Processing Industrial Byproducts as Raw Material for the Production of Plant Protein Foods ». Dans Plant Protein Foods, 109–29. Cham : Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-91206-2_4.

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Kunz, Meik. « Material und Methoden ». Dans Modellierung und Simulation von Protein-Interaktionen am Beispiel von Wirts-Pathogen-Interaktionen, 43–48. Wiesbaden : Springer Fachmedien Wiesbaden, 2017. http://dx.doi.org/10.1007/978-3-658-16778-3_2.

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Schiesser, William E. « Virus Protein ODE/PDE Models ». Dans Virus Host Cell Genetic Material Transport, 1–4. Cham : Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-68865-3_1.

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Mills, O. E., et A. J. Broome. « Isolation of Flavor Compounds from Protein Material ». Dans ACS Symposium Series, 85–91. Washington, DC : American Chemical Society, 1998. http://dx.doi.org/10.1021/bk-1998-0705.ch008.

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Maschke, A., A. Lucke, W. Vogelhuber, C. Fischbach, B. Appel, T. Blunk et A. Göpferich. « Lipids : An Alternative Material for Protein and Peptide Release ». Dans ACS Symposium Series, 176–96. Washington, DC : American Chemical Society, 2004. http://dx.doi.org/10.1021/bk-2004-0879.ch013.

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Ward, C. A. « Blood Protein-Material Interactions That Lead to Cellular Adhesion ». Dans ACS Symposium Series, 551–65. Washington, DC : American Chemical Society, 1987. http://dx.doi.org/10.1021/bk-1987-0343.ch034.

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Blaich, Rolf. « Function of Genetic Material Transposable Elements, Seed Proteins, and Protein Synthesis in Higher Plants ». Dans Progress in Botany, 198–207. Berlin, Heidelberg : Springer Berlin Heidelberg, 1985. http://dx.doi.org/10.1007/978-3-642-45607-7_14.

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Actes de conférences sur le sujet "Protein material"

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Teng, Weibing, Joseph Cappello et Xiaoyi Wu. « Viscoelastic Properties of Genetically Engineered Silk-Elastin-Like Protein Polymers ». Dans ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192252.

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Genetic engineering of protein-based materials provides material scientists with high levels of control in material microstructures, properties, and functions [1]. For example, multi-block protein copolymers in which individual block may possess distinct mechanical or biological properties have been biosynthesized [2, 3]. Polypeptide sequences derived from well-studied structural proteins (e.g., collagen, silk, elastin) are often used as motifs in the design and synthesis of new protein-based material, in which new functional groups may be incorporated. In this fashion, we have produced a series of silk-elastin-like proteins (SELPs) consisting of polypeptide sequences derived from silk of superior mechanical strength and elastin that is extremely durable and resilient [2, 4]. Notably, the silk-like blocks are capable of crystallizing to form virtual cross-links between elastin-mimetic sequences, which, in turn, lower the crystallinity of the silk-like blocks and thus enhance the solubility of SELPs. Consequently, SELPs may be fabricated into useful structures for biomedical applications, including drug delivery. In this study, we will characterize viscoelastic properties of SELPs, which are particularly relevant to tissue engineering applications.
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Panda, Rupayana, Satya Narayan Sahu, Fahmida Khan et Subrat Kumar Pattanayak. « Binding performance of phytochemicals with mutant threonine-protein kinase Chk2 protein : An in silico study ». Dans PROCEEDINGS OF ADVANCED MATERIAL, ENGINEERING & TECHNOLOGY. AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0019655.

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Lin, Zhen-Rong, Yun-Yun Xu et Tao Zhang. « Application of Nanometer Materials in Protein Separation ». Dans 3rd Annual International Conference on Advanced Material Engineering (AME 2017). Paris, France : Atlantis Press, 2017. http://dx.doi.org/10.2991/ame-17.2017.50.

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Lisnichenko, Marina, et Stanislav Protasov. « BIO MATERIAL MODELING QUANTUM CIRCUIT COMPRESSION ». Dans Mathematical modeling in materials science of electronic component. LCC MAKS Press, 2022. http://dx.doi.org/10.29003/m3058.mmmsec-2022/15-17.

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Bioelectronics is a perspective future of electronics. The modelling of the protein is an important part that allows to search the appropriate folding structure with applicable conductivity properties. The classical computers struggle from modelling large structures because of number degrees of freedom. The mathematical modelling inside the quantum programming paradigm is a possible way to overcome this factor. This work describes a simplification algorithm of bio material (protein) model used in bioelectronics
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Munthe, Eika Abigail, Saronom Silaban et Zainuddin Muchtar. « Discovery Learning Based E-Module on Protein Material Development ». Dans Proceedings of the 4th Annual International Seminar on Transformative Education and Educational Leadership (AISTEEL 2019). Paris, France : Atlantis Press, 2019. http://dx.doi.org/10.2991/aisteel-19.2019.137.

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Shawkat Zamil, K. M., et Julia Rahman. « Prediction of Protein-Protein Interaction from Amino Acid Sequence Using Ensemble Classifier ». Dans 2018 International Conference on Computer, Communication, Chemical, Material and Electronic Engineering (IC4ME2). IEEE, 2018. http://dx.doi.org/10.1109/ic4me2.2018.8465485.

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Mattern-Schain, Samuel I., Mary-Anne Nguyen, Tayler M. Schimel, James Manuel, Joshua Maraj, Donald Leo, Eric Freeman, Scott Lenaghan et Stephen A. Sarles. « Totipotent Cellularly-Inspired Materials ». Dans ASME 2019 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/smasis2019-5745.

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Abstract This work draws inspiration from totipotent cellular systems to design smart materials whose compositions and properties can be learned or evolved. Totipotency refers to the inherent genetic potential of a single cell to adapt and produce all types of differentiated cells within an organism. To study this principal and apply it synthetically, tissue-like compartmentalized assemblies are constructed via lipid membrane-separated aqueous droplets in a hydrophobic medium through the droplet interface bilayer (DIB) method. Within our droplets, we explore synthetic totipotency via cell-free reactions including actin polymerization and cell free protein synthesis (CFPS). The transcription and translation of our CFPS reactions are controlled by stimuli-responsive riboswitches (RS). Via this scheme, adaptable material properties and functions are achieved in vitro via protein production from cell-free machinery administered through RS governance. Here, we present thermally or chemically-triggered riboswitches for orthogonal production of representative fluorescent protein products, as well functional proteins. To characterize the material properties of target proteins, we study the formation of polymerized actin shells to stabilize organically-encased droplets and span DIBs. We present a modified protocol for chemically-triggered actin polymerization as well as a thermally triggered actin RS. We characterize theophylline (TP)-triggered production of alpha hemolysin (α-HL) through CFPS and synthesized an organic-soluble trigger that can be sensed from the oil phase by a RS in an aqueous bioreactor droplet. We also demonstrate increased droplet conductivity when CFPS α-HL products are incorporated in DIBs. This interdisciplinary work involves cell culture, gene expression, organic synthesis, vesicle formation, protein quantification, tensiometry, droplet aspiration, microplate fluorescence/absorption experiments, fluorescent microscopy, and electrophysiology. This project is an essential design analysis for creating smart, soft materials using synthetic biology and provides motivation for artificial tissues capable of adapting in response to external stimuli.
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Ott, Lindsey, Cindy Vu, Ashley Farris, Robert Weatherly et Michael Detamore. « Material Composition Gradients and Protein Release for Tracheal Defect Repair ». Dans ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14391.

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Windpipe defects result in decreased quality of life for the patient, making breathing, speaking, and swallowing difficult. Disorders of the trachea requiring intervention methods not adequately treated by slide tracheoplasty or cartilage augmentation necessitate the use of prosthetic material to expand the trachea. Furthermore, some donor site morbidity occurs with augmentation techniques and size or shape mismatches are not uncommon. Tissue engineering has the potential to create effective replacement trachea-like tissue for procedures like laryngotracheal reconstruction and may circumvent these problems.
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Jafar Khan Kasi, Ajab Khan Kasi, Nitin Afzulpurkar et Naveed Sheikh. « Notice of Retraction : Protein sensor for the waste dialysate material ». Dans 2010 2nd International Conference on Mechanical and Electronics Engineering (ICMEE 2010). IEEE, 2010. http://dx.doi.org/10.1109/icmee.2010.5558420.

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Pratakshya, Preeta, et Alon Gorodetsky. « Tunable Assembly and Refractive Index of a Cephalopod Protein-Based Material ». Dans Novel Optical Materials and Applications. Washington, D.C. : OSA, 2021. http://dx.doi.org/10.1364/noma.2021.nom1d.4.

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Rapports d'organisations sur le sujet "Protein material"

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Lu, Hong P. Controlling Protein Conformations to Explore Unprecedented Material Properties by Single-Molecule Surgery. Fort Belvoir, VA : Defense Technical Information Center, août 2012. http://dx.doi.org/10.21236/ada584676.

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Evans, John Spencer. Material lessons of biology : structure function studies of protein sequences involved in inorganic composite material formation. Final Technical Report. Office of Scientific and Technical Information (OSTI), septembre 2019. http://dx.doi.org/10.2172/1560814.

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Dea, Jack Y., et Lev Sheiba. Material and Acoustical Studies of Elastic Protein-Based Polymers Engineered for Selected Acoustical and Non-Acoustical Characteristics. Fort Belvoir, VA : Defense Technical Information Center, juin 2002. http://dx.doi.org/10.21236/ada403518.

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Gafny, Ron, A. L. N. Rao et Edna Tanne. Etiology of the Rugose Wood Disease of Grapevine and Molecular Study of the Associated Trichoviruses. United States Department of Agriculture, septembre 2000. http://dx.doi.org/10.32747/2000.7575269.bard.

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Rugose wood is a complex disease of grapevines, characterized by modification of the woody cylinder of affected vines. The control of rugose wood is based on the production of healthy propagation material. Detection of rugose wood in grapevines is difficult and expensive: budwood from tested plants is grafted onto sensitive Vitis indicators and the appearance of symptoms is monitored for 3 years. The etiology of rugose wood is complex and has not yet been elucidated. Several elongated clostero-like viruses are consistently found in affected vines; one of them, grapevine virus A (GVA), is closely associated with Kober stem grooving, a component of the rugose wood complex. GVA has a single-stranded RNA genome of 7349 nucleotides, excluding a polyA tail at the 3' terminus. The GVA genome includes five open reading frames (ORFs 1-5). ORF 4, which encodes for the coat protein of GVA, is the only ORF for which the function was determined experimentally. The original objectives of this research were: 1- To produce antisera to the structural and non-structural proteins of GVA and GVB and to use these antibodies to establish an effective detection method. 2- Develop full length infectious cDNA clones of GVA and GVB. 3- Study the roll of GVA and GVB in the etiology of the grapevine rugose wood disease. 4- Determine the function of Trichovirus (now called Vitivirus) encoded genes in the virus life cycle. Each of the ORFs 2, 3, 4 and 5 genes of GVA were cloned and expressed in E. coli and used to produce antisera. Both the CP (ORF 4) and the putative MP (ORF 3) were detected with their corresponding antisera in-GVA infected N. benthamiana and grapevine. The MP was first detected at an early stage of the infection, 6-12 h after inoculation, and the CP 2-3 days after inoculation. The MP could be detected in GVA-infected grapevines that tested negative for CP, both with CP antiserum and with a commercially available ELISA kit. Antisera to ORF 2 and 5 encoded proteins could react with the recombinant proteins but failed to detect both proteins in GVA infected plants. A full-length cDNA clone of grapevine virus A (GVA) was constructed downstream from the bacteriophage T7 RNA polymerase promoter. Capped in vitro transcribed RNA was infectious in N. benthamiana and N. clevelandii plants. Symptoms induced by the RNA transcripts or by the parental virus were indistinguishable. The infectivity of the in vitro-transcribed RNA was confirmed by serological detection of the virus coat and movement proteins and by observation of virions by electron microscopy. The full-length clone was modified to include a gus reporter gene and gus activity was detected in inoculated and systemic leaves of infected plants. Studies of GVA mutants suggests that the coat protein (ORF 4) is essential for cell to cell movement, the putative movement protein (ORF 3) indeed functions as a movement protein and that ORF 2 is not required for virus replication, cell to cell or systemic movement. Attempts to infect grapevines by in-vitro transcripts, by inoculation of cDNA construct in which the virus is derived by the CaMV 35S promoter or by approach grafting with infected N. benthamiana, have so far failed. Studies of the subcellular distribution of GFP fusion with each of ORF 2, 3 and 4 encoded protein showed that the CP fusion protein accumulated as a soluble cytoplasmatic protein. The ORF 2 fusion protein accumulated in cytoplasmatic aggregates. The MP-GFP fusion protein accumulated in a large number of small aggregates in the cytoplasm and could not move from cell to cell. However, in conditions that allowed movement of the fusion protein from cell to cell (expression by a PVX vector or in young immature leaves) the protein did not form cytoplasmatic aggregates but accumulated in the plasmodesmata.
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Barakat, Dr Shima, Dr Samuel Short, Dr Bernhard Strauss et Dr Pantea Lotfian. https://www.food.gov.uk/research/research-projects/alternative-proteins-for-human-consumption. Food Standards Agency, juin 2022. http://dx.doi.org/10.46756/sci.fsa.wdu243.

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The UK is seeing growing interest in alternative protein sources to traditional animal-based proteins such as beef, lamb, pork, poultry, fish, eggs, and dairy. There is already an extensive market in alternative protein materials, however, technological advances combined with the pressure for more sustainable sources of protein has led to an acceleration of innovation and product development and the introduction of a large amount of new alternative protein ingredients and products to the market. These have the potential to dramatically impact on the UK food system. This report is a combination of desk research, based on thorough review of the academic and non-academic literature and of the alternative proteins start-up scene, and presents an analysis of the emerging market for alternative proteins, the potential implications and the potential policy responses that the FSA might need to consider. Four main categories of alternative proteins are presented and reviewed in this report: Plant-based meat substitutes Novel protein sources Proteins and biomass biosynthesised by microorganisms Cultured meat
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Barefoot, Susan F., Bonita A. Glatz, Nathan Gollop et Thomas A. Hughes. Bacteriocin Markers for Propionibacteria Gene Transfer Systems. United States Department of Agriculture, juin 2000. http://dx.doi.org/10.32747/2000.7573993.bard.

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The antibotulinal baceriocins, propionicin PLG-1 and jenseniin G., were the first to be identified, purified and characterized for the dairy propionibaceria and are produced by Propionibacterium thoenii P127 and P. thoenii/jensenii P126, respectively. Objectives of this project were to (a) produce polyclonal antibodies for detection, comparison and monitoring of propionicin PLG-1; (b) identify, clone and characterize the propionicin PLG-1 (plg-1) and jenseniin G (jnG) genes; and (3) develop gene transfer systems for dairy propionibacteria using them as models. Polyclonal antibodies for detection, comparison and monitoring of propionicin PLG-1 were produced in rabbits. Anti-PLG-1 antiserum had high titers (256,000 to 512,000), neutralized PLG-1 activity, and detected purified PLG-1 at 0.10 mg/ml (indirect ELISA) and 0.033 mg/ml (competitive indirect ELISA). Thirty-nine of 158 strains (most P. thoenii or P. jensenii) yielded cross-reacting material; four strains of P. thoenii, including two previously unidentified bacteriocin producers, showed biological activity. Eight propionicin-negative P127 mutants produced neither ELISA response nor biological activity. Western blot analyses of supernates detected a PLG-1 band at 9.1 kDa and two additional protein bands with apparent molecular weights of 16.2 and 27.5 kDa. PLG-1 polyclonal antibodies were used for detection of jenseniin G. PLG-1 antibodies neutralized jenseniin G activity and detected a jenseniin G-sized, 3.5 kDa peptide. Preliminary immunoprecipitation of crude preparations with PLG-1 antibodies yielded three proteins including an active 3-4 kDa band. Propionicin PLG-1 antibodies were used to screen a P. jensenii/thoenii P126 genomic expression library. Complete sequencing of a cloned insert identified by PLG-1 antibodies revealed a putative response regulator, transport protein, transmembrane protein and an open reading frame (ORF) potentially encoding jenseniin G. PCR cloning of the putative plg-1 gene yielded a 1,100 bp fragment with a 355 bp ORF encoding 118 amino acids; the deduced N-terminus was similar to the known PLG-1 N-terminus. The 118 amino acid sequence deduced from the putative plg-1 gene was larger than PLG-1 possibly due to post-translational processing. The product of the putative plg-1 gene had a calculated molecular weight of 12.8 kDa, a pI of 11.7, 14 negatively charged residues (Asp+Glu) and 24 positively charged residues (Arg+Lys). The putative plg-1 gene was expressed as an inducible fusion protein with a six-histidine residue tag. Metal affinity chromatography of the fused protein yielded a homogeneous product. The fused purified protein sequence matched the deduced putative plg-1 gene sequence. The data preliminarily suggest that both the plg-1 and jnG genes have been identified and cloned. Demonstrating that antibodies can be produced for propionicin PLG-1 and that those antibodies can be used to detect, monitor and compare activity throughout growth and purification was an important step towards monitoring PLG-1 concentrations in food systems. The unexpected but fortunate cross-reactivity of PLG-1 antibodies with jenseniin G led to selective recovery of jenseniin G by immunoprecipitation. Further refinement of this separation technique could lead to powerful affinity methods for rapid, specific separation of the two bacteriocins and thus facilitate their availability for industrial or pharmaceutical uses. Preliminary identification of genes encoding the two dairy propionibacteria bacteriocins must be confirmed; further analysis will provide means for understanding how they work, for increasing their production and for manipulating the peptides to increase their target species. Further development of these systems would contribute to basic knowledge about dairy propionibacteria and has potential for improving other industrially significant characteristics.
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Crisosto, Carlos, Susan Lurie, Haya Friedman, Ebenezer Ogundiwin, Cameron Peace et George Manganaris. Biological Systems Approach to Developing Mealiness-free Peach and Nectarine Fruit. United States Department of Agriculture, 2007. http://dx.doi.org/10.32747/2007.7592650.bard.

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Peach and nectarine production worldwide is increasing; however consumption is flat or declining because of the inconsistent eating quality experienced by consumers. The main factor for this inconsistent quality is mealiness or woolliness, a form of chilling injury that develops following shipping periods in the global fruit market today. Our research groups have devised various postharvest methods to prolong storage life, including controlled atmosphere and delayed storage; however, these treatments only delay mealiness. Mealiness texture results from disruption of the normal ripening process involving disassembly of cell wall material, and creates a soft fruit texture that is dry and grainy instead of juicy and smooth. Solving this problem is a prerequisite for increasing the demand for fresh peach and nectarine. Two approaches were used to reveal genes and their associated biochemical processes that can confer resistance to mealiness or wooliness. At the Volcani Center, Israel, a nectarine cultivar and the peach cultivar (isogenetic materials) from which the nectarine cultivar spontaneously arose, and at the Kearney Agricultural Center of UC Davis, USA, a peach population that segregates for quantitative resistance to mealiness was used for dissecting the genetic components of mealiness development. During our project we have conducted research integrating the information from phenotypic, biochemical and gene expression studies, proposed possible candidate genes and SNPs-QTLs mapping that are involved in reducing peach mealiness susceptibility. Numerous genes related to ethylene biosynthesis and its signal transduction, cell wall structure and metabolism, stress response, different transcription factor families were detected as being differentially accumulated in the cold-treated samples of these sensitive and less sensitive genotypes. The ability to produce ethylene and keep active genes involved in ethylene signaling, GTP-binding protein, EIN-3 binding protein and an ethylene receptor and activation of ethyleneresponsive fruit ripening genes during cold storage provided greater resistance to CI. Interestingly, in the functional category of genes differentially expressed at harvest, less chilling sensitive cultivar had more genes in categories related to antioxidant and heat sock proteins/chaperones that may help fruit to adapt to low temperature stress. The specific objectives of the proposed research were to: characterize the phenotypes and cell wall components of the two resistant systems in response to mealiness- inducing conditions; identify commonalities and specific differences in cell wall proteins and the transcriptome that are associated with low mealiness incidence; integrate the information from phenotypic, biochemical, and gene expression studies to identify candidate genes that are involved in reducing mealiness susceptibility; locate these genes in the Prunus genome; and associate the genes with genomic regions conferring quantitative genetic variation for mealiness resistance. By doing this we will locate genetic markers for mealiness development, essential tools for selection of mealiness resistant peach lines with improved fruit storability and quality. In our research, QTLs have been located in our peach SNPs map, and proposed candidate genes obtained from the integrated result of phenotypic, biochemical and gene expression analysis are being identified in our QTLs as an approach searching for consistent assistant markers for peach breeding programs.
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Smith, G. S., A. Nowak et C. Safinya. Advanced biomolecular materials based on membrane-protein/polymer complexation. Office of Scientific and Technical Information (OSTI), décembre 1998. http://dx.doi.org/10.2172/296874.

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Lewis, Randolph V. Designing Spider Silk Proteins for Materials Applications. Fort Belvoir, VA : Defense Technical Information Center, octobre 2009. http://dx.doi.org/10.21236/ada516656.

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Short, Samuel, Bernhard Strauss et Pantea Lotfian. Emerging technologies that will impact on the UK Food System. Food Standards Agency, juin 2021. http://dx.doi.org/10.46756/sci.fsa.srf852.

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Rapid technological innovation is reshaping the UK food system in many ways. FSA needs to stay abreast of these changes and develop regulatory responses to ensure novel technologies do not compromise food safety and public health. This report presents a rapid evidence assessment of the emerging technologies considered most likely to have a material impact on the UK food system and food safety over the coming decade. Six technology fields were identified and their implications for industry, consumers, food safety and the regulatory framework explored. These fields are: Food Production and Processing (indoor farming, 3D food printing, food side and byproduct use, novel non-thermal processing, and novel pesticides); Novel Sources of Protein, such as insects (for human consumption, and animal feedstock); Synthetic Biology (including lab-grown meat and proteins); Genomics Applications along the value chain (for food safety applications, and personal “nutrigenomics”); Novel Packaging (active, smart, biodegradable, edible, and reusable solutions); and, Digital Technologies in the food sector (supporting analysis, decision making and traceability). The report identifies priority areas for regulatory engagement, and three major areas of emerging technology that are likely to have broad impact across the entire food industry. These areas are synthetic biology, novel food packaging technologies, and digital technologies. FSA will need to take a proactive approach to regulation, based on frequent monitoring and rapid feedback, to manage the challenges these technologies present, and balance increasing technological push and commercial pressures with broader human health and sustainability requirements. It is recommended FSA consider expanding in-house expertise and long-term ties with experts in relevant fields to support policymaking. Recognising the convergence of increasingly sophisticated science and technology applications, alongside wider systemic risks to the environment, human health and society, it is recommended that FSA adopt a complex systems perspective to future food safety regulation, including its wider impact on public health. Finally, the increasing pace of technological
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