Bibliographies thématiques / Protein functionalization

Littérature scientifique sur le sujet « Protein functionalization »

Auteur : Grafiati
Publié le 7 juillet 2024
Mis à jour le 7 juillet 2024

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Articles de revues sur le sujet "Protein functionalization"

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Mateu, M. G. « Virus engineering : functionalization and stabilization ». Protein Engineering Design and Selection 24, no 1-2 (5 octobre 2010) : 53–63. http://dx.doi.org/10.1093/protein/gzq069.

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Crasson, O., N. Rhazi, O. Jacquin, A. Freichels, C. Jérôme, N. Ruth, M. Galleni, P. Filée et M. Vandevenne. « Enzymatic functionalization of a nanobody using protein insertion technology ». Protein Engineering Design and Selection 28, no 10 (6 avril 2015) : 451–60. http://dx.doi.org/10.1093/protein/gzv020.

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Yoon, Sungkwon, et William T. Nichols. « Nano-functionalization of protein microspheres ». Applied Surface Science 309 (août 2014) : 106–11. http://dx.doi.org/10.1016/j.apsusc.2014.04.194.

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Wang, Ruidi, Linglan Fu, Junqiu Liu et Hongbin Li. « Decorating protein hydrogels reversibly enables dynamic presentation and release of functional protein ligands on protein hydrogels ». Chemical Communications 55, no 84 (2019) : 12703–6. http://dx.doi.org/10.1039/c9cc06374a.

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Permana, Dani, Herlian Eriska Putra et Djaenudin Djaenudin. « Designed protein multimerization and polymerization for functionalization of proteins ». Biotechnology Letters 44, no 3 (27 janvier 2022) : 341–65. http://dx.doi.org/10.1007/s10529-021-03217-8.

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Paolino, Marco, Michela Visintin, Elisa Margotti, Marco Visentini, Laura Salvini, Annalisa Reale, Vincenzo Razzano et al. « Functionalization of protein hexahistidine tags by functional nanoreactors ». New Journal of Chemistry 43, no 46 (2019) : 17946–53. http://dx.doi.org/10.1039/c9nj03463c.

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Meredith, Gavin D., Hayley Y. Wu et Nancy L. Allbritton. « Targeted Protein Functionalization Using His-Tags ». Bioconjugate Chemistry 15, no 5 (septembre 2004) : 969–82. http://dx.doi.org/10.1021/bc0498929.

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Naskar, Nilanjon, Martin F. Schneidereit, Florian Huber, Sabyasachi Chakrabortty, Lothar Veith, Markus Mezger, Lutz Kirste et al. « Impact of Surface Chemistry and Doping Concentrations on Biofunctionalization of GaN/Ga‒In‒N Quantum Wells ». Sensors 20, no 15 (28 juillet 2020) : 4179. http://dx.doi.org/10.3390/s20154179.

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The development of sensitive biosensors, such as gallium nitride (GaN)-based quantum wells, transistors, etc., often makes it necessary to functionalize GaN surfaces with small molecules or even biomolecules, such as proteins. As a first step in surface functionalization, we have investigated silane adsorption, as well as the formation of very thin silane layers. In the next step, the immobilization of the tetrameric protein streptavidin (as well as the attachment of chemically modified iron transport protein ferritin (ferritin-biotin-rhodamine complex)) was realized on these films. The degree of functionalization of the GaN surfaces was determined by fluorescence measurements with fluorescent-labeled proteins; silane film thickness and surface roughness were estimated, and also other surface sensitive techniques were applied. The formation of a monolayer consisting of adsorbed organosilanes was accomplished on Mg-doped GaN surfaces, and also functionalization with proteins was achieved. We found that very high Mg doping reduced the amount of surface functionalized proteins. Most likely, this finding was a consequence of the lower concentration of ionizable Mg atoms in highly Mg-doped layers as a consequence of self-compensation effects. In summary, we could demonstrate the necessity of Mg doping for achieving reasonable bio-functionalization of GaN surfaces.
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De Geyter, Ewout, Eirini Antonatou, Dimitris Kalaitzakis, Sabina Smolen, Abhishek Iyer, Laure Tack, Emiel Ongenae, Georgios Vassilikogiannakis et Annemieke Madder. « 5-Hydroxy-pyrrolone based building blocks as maleimide alternatives for protein bioconjugation and single-site multi-functionalization ». Chemical Science 12, no 14 (2021) : 5246–52. http://dx.doi.org/10.1039/d0sc05881e.

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Recent expansion in potential uses of protein conjugates has fueled the development of a range of protein modification methods; however, the desirable single-site multi-functionalization of proteins has remained a particularly intransigent challenge.
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Guzmán-Mendoza, José Jesús, David Chávez-Flores, Silvia Lorena Montes-Fonseca, Carmen González-Horta, Erasmo Orrantia-Borunda et Blanca Sánchez-Ramírez. « A Novel Method for Carbon Nanotube Functionalization Using Immobilized Candida antarctica Lipase ». Nanomaterials 12, no 9 (26 avril 2022) : 1465. http://dx.doi.org/10.3390/nano12091465.

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Carbon nanotubes (CNTs) have been proposed as nanovehicles for drug or antigen delivery since they can be functionalized with different biomolecules. For this purpose, different types of molecules have been chemically bonded to CNTs; however, this method has low efficiency and generates solvent waste. Candida antarctica lipase is an enzyme that, in an organic solvent, can bind a carboxylic to a hydroxyl group by esterase activity. The objective of this work was to functionalize purified CNTs with insulin as a protein model using an immobilized lipase of Candida antarctica to develop a sustainable functionalization method with high protein attachment. The functionalized CNTs were characterized by scanning electron microscope (SEM), Raman spectroscopy, Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE). The enzymatic functionalization of insulin on the surface of the CNTs was found to have an efficiency of 21%, which is higher in conversion and greener than previously reported by the diimide-activated amidation method. These results suggest that enzymatic esterification is a convenient and efficient method for CNT functionalization with proteins. Moreover, this functionalization method can be used to enhance the cellular-specific release of proteins by lysosomal esterases.
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Thèses sur le sujet "Protein functionalization"

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Buck, Chelsea. « Characterization and Functionalization of Suckerin-12 Protein Hydrogels ». University of Dayton / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1542729200115677.

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Takeda, Shigeo. « Functionalization of Glucan Dendrimers and Bio-applications ». Kyoto University, 2020. http://hdl.handle.net/2433/253505.

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Tabe, Hiroyasu. « Studies on Functionalization of Porous Protein Crystals by Immobilizing Organometallic Complexes ». 京都大学 (Kyoto University), 2015. http://hdl.handle.net/2433/200445.

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Yildirim, Eda Didem Sun Wei Guceri S. I. « Plasma and protein surface functionalization for three-dimensional polycaprolactone tissue scaffolds / ». Philadelphia, Pa. : Drexel University, 2010. http://hdl.handle.net/1860/3326.

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Takaoka, Yosuke. « Development of New Methods for Chemical Labeling, Functionalization and Detection of Proteins by Ligand-tethered Probes ». 京都大学 (Kyoto University), 2010. http://hdl.handle.net/2433/120896.

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Ahmad, Asad Ali. « Surface Functionalization and Analysis Thereof for an Ovarian Cancer Diagnostic Biosensor ». Scholar Commons, 2011. http://scholarcommons.usf.edu/etd/2977.

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Ovarian cancer is the fifth leading cause of cancer death among women in United States and has an alarming 1.4% (1 in 71) lifetime risk. The lack of overt symptoms and the absence of a reliable screening test to detect ovarian cancer result in over 70% of women being diagnosed after the disease has spread beyond the ovary resulting in a poor prognosis. A key characteristic of ovarian cancer is the ability of tumor cells to evade apoptosis, or programmed cell death contributing to the limitless replicative potential, which is a hallmark of all carcinogenesis. There is conclusive evidence that levels of bcl-2 are elevated in ovarian cancer patients' indication that this protein is an ovarian cancer biomarker. The overall goal of this thesis is to functionalize a substrate for specific, sensitive and cost-effective bcl-2 capture. This surface will ultimately be incorporated into an acoustic wave-based diagnostic device for worldwide point-of-care (POC) ovarian cancer detection. This research looks to assess the capture of this analyte protein on a series of bioconjugated surfaces. For the research to be diagnostically applicable, certain factors reveal themselves as more important than others. Since the surface-bound capture antibody must recognize the bcl-2 protein, it is vital to ensure upright orientation of this specific antibody with high affinity for the analyte. Furthermore once integrated with a nanosensor, the surface will sense a change in the mass on the surface, which requires that the surface is highly resistant to non-specific binding. Bioconjugation techniques were employed to initiate self-assembled monolayers (SAM) of silanes, immobilize antibodies (via amine-crosslinking or direct adsorption of protein A/G) and disperse polyethylene glycol (PEG) reagents to reduce non-specific binding on the glass substrates. 3-aminopropyltrimethoxysilane (3-APTMS) and chlorodimethyloctylsilane (ODMS) were deposited on the surface to create initial hydrophilic and hydrophobic properties on which molecular self-assembly could occur. Testing a variety of assemblies with and without the presence of silanes, amine-crosslinking and PEGylation reagents, the substrate displaying the highest efficacy of bcl-2 capture was revealed. These various surfaces were assessed through contact angle and a novel sandwich enzyme linked immunosorbent assay (ELISA) for sensitivity and specificity of bcl-2 standard capture. The consistently low background and facile assembly of the ODMS based substrate with direct adsorption of protein A/G and the PEGylation reagent, Pluronic, was deemed the best functionalized surface for non-specific recruitment of the bcl-2 protein. The substrate also consistently displayed low signal-to-noise ratio which was of extreme importance in this research to guarantee the prevention of false-positive results when detecting nascent carcinogenic behavior. Elucidation of this substrate assembly is the first step towards the long term objective of this thesis, which is to construct a cost-effective early ovarian cancer detection device which can be implemented at the point-of-care to those who need it the most. This is ultimately expected to dramatically improve health outcomes for females worldwide.
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Cai, Yixiao. « Bio-Nano Interactions : Synthesis, Functionalization and Characterization of Biomaterial Interfaces ». Doctoral thesis, Uppsala universitet, Tillämpad materialvetenskap, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-277121.

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Current strategies for designing biomaterials involve creating materials and interfaces that interact with biomolecules, cells and tissues.  This thesis aims to investigate several bioactive surfaces, such as nanocrystalline diamond (NCD), hydroxyapatite (HA) and single crystalline titanium dioxide, in terms of material synthesis, surface functionalization and characterization. Although cochlear implants (CIs) have been proven to be clinically successful, the efficiency of these implants still needs to be improved. A CI typically only has 12-20 electrodes while the ear has approximately 3400 inner hair cells. A type of micro-textured NCD surface that consists of micrometre-sized nail-head-shaped pillars was fabricated. Auditory neurons showed a strong affinity for the surface of the NCD pillars, and the technique could be used for neural guidance and to increase the number of stimulation points, leading to CIs with improved performance. Typical transparent ceramics are fabricated using pressure-assisted sintering techniques. However, the development of a simple energy-efficient production method remains a challenge. A simple approach to fabricating translucent nano-ceramics was developed by controlling the morphology of the starting ceramic particles. Translucent nano-ceramics, including HA and strontium substituted HA, could be produced via a simple filtration process followed by pressure-less sintering. Furthermore, the application of such materials as a window material was investigated. The results show that MC3T3 cells could be observed through the translucent HA ceramic for up to 7 days. The living fluorescent staining confirmed that the MC3T3 cells were visible throughout the culture period. Single crystalline rutile possesses in vitro bioactivity, and the crystalline direction affects HA formation. The HA growth on (001), (100) and (110) faces was investigated in a simulated body fluid in the presence of fibronectin (FN) via two different processes. The HA layers on each face were analysed using different characterization techniques, revealing that the interfacial energies could be altered by the pre-adsorbed FN, which influenced HA formation. In summary, micro textured NCD, and translucent HA and FN functionalized single crystalline rutile, and their interactions with cells and biomimetic HA were studied. The results showed that controlled surface properties are important for enhancing a material’s biological performance.
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Darwish, Amina M. « Silica Surface Modifications for Protein Separation ». University of Cincinnati / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1416231191.

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Schumacher, Dominik. « Site-specific functionalization of antigen binding proteins for cellular delivery, imaging and target modulation ». Doctoral thesis, Humboldt-Universität zu Berlin, 2017. http://dx.doi.org/10.18452/18547.

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Antikörper und Antigen-bindende Proteine, die an Fluorophore, Tracer und Wirkstoffe konjugiert sind, sind einzigartige Moleküle, welche die Entwicklung wertvoller diagnostischer und therapeutischer Werkzeuge ermöglichen. Allerdings ist der Konjugationsschritt sehr anspruchsvoll und trotz intensiver Forschung noch immer ein bedeutender Engpass. Zusätzlich sind Antigen-bindende Proteine oftmals nicht dazu in der Lage, die Zellmembran zu durchdringen und im Zellinneren nicht funktionsfähig. Daher ist ihre Verwendung auf extrazelluläre Targets beschränkt, was eine bedeutende Anzahl wichtiger Antigene vernachlässigt. Beide Limitierungen bilden Kernaspekte dieser Arbeit. Mit Tub-tag labeling wurde ein neuartiges und vielseitiges Verfahren für die ortsspezifische Funktionalisierung von Biomolekülen und Antigen-bindenden Proteinen entwickelt, und so die Palette der Proteinfunktionalisierungen bedeutend erweitert. Tub-tag wurde erfolgreich für die ortsspezifische Funktionalisierung verschiedener Proteine und Antigen-bindender Nanobodies angewendet, die für konfokale Mikroskopie, Proteinanreicherung und hochauflösende Mikroskopie eingesetzt wurden. In einem weiteren Projekt wurden zellpermeable Antigen-bindende Nanobodies hergestellt und somit das schon lange Zeit bestehende Ziel, intrazelluläre Targets durch in vitro funktionalisierte Antigen-bindende Proteine zu visualisieren und manipulieren, erreicht. Hierzu wurden zwei verschiedene Nanobodies an ihrem C-Terminus cyclischen zellpenetrierenden Peptiden unter Verwendung von Expressed Protein Ligation funktionalisiert. Diese Peptide ermöglichten die Endozytose-unabhängige Aufnahme der Nanobodies mit sofortiger Bioverfügbarkeit. Mit Tub-tag labeling und der Synthese von zellpermeablen Nanobodies konnten wichtige Bottlenecks im Bereich der Proteinfunktionalisierung und Antikörperforschung adressiert werden und neue Tools für die biochemische und zellbiologische Forschung entwickelt werden.
Antibodies and antigen binding proteins conjugated to fluorophores, tracers and drugs are powerful molecules that enabled the development of valuable diagnostic and therapeutic tools. However, the conjugation itself is highly challenging and despite intense research efforts remains a severe bottleneck. In addition to that, antibodies and antigen binding proteins are often not functional within cellular environments and unable to penetrate the cellular membrane. Therefore, their use is limited to extracellular targets leaving out a vast number of important antigens. Both limitations are core aspects of the presented thesis. With Tub-tag labeling, a novel and versatile method for the site-specific functionalization of biomolecules and antigen binding proteins was developed expanding the toolbox of protein functionalization. The method is based on the microtubule enzyme tubulin tyrosine ligase. Tub-tag labeling was successfully applied for the site-specific functionalization of different proteins including antigen binding nanobodies which enabled confocal microscopy, protein enrichment and super-resolution microscopy. In addition to that, cell permeable antigen binding nanobodies have been generated constituting a long thought goal of tracking and manipulating intracellular targets by in vitro functionalized antigen binding proteins. To achieve this goal, two different nanobodies were functionalized at their C-terminus with linear and cyclic cell-penetrating peptides using expressed protein ligation. These peptides triggered the endocytosis independent uptake of the nanobodies with immediate bioavailability. Taken together, Tub-tag labeling and the generation of cell-permeable antigen binding nanobodies strongly add to the functionalization of antibodies and their use in biochemistry, cell biology and beyond.
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Lella, Divya Jyothi. « Functionalization and Modification of Naphthaquinone Analogs as HER2 Kinase Inhibitors ». TopSCHOLAR®, 2014. http://digitalcommons.wku.edu/theses/1325.

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HER2 overexpression in breast cancer tumors predicts lower overall survival. Because of the aggressive nature of HER2 tumors and the association with metastatic disease, the HER2 receptor holds great promise as a therapeutic target in metastatic breast cancer. We are developing small molecule inhibitors that bind to the ATP binding site of the tyrosine kinase domain in order to inhibit tyrosine auto-phosphorylation. This process controls biological pathways that mediate the cell growth. In normal cells this process is highly controlled. We are targeting the modification of the side chain of the hydroxy methyl group of 2-Hydroxy methyl-5,8-dimethoxy-1,4-naphthaquinone. These compounds should inhibit the tyrosine kinase cascade of reactions thereby suppressing the overexpression of HER2 shutting down the tumor growth. The synthesis and characterization of a series of substituted naphthaquinone analogs with different increasing chain lengths will be reported.
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Chapitres de livres sur le sujet "Protein functionalization"

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Colpo, Pascal, Ana Ruiz, Laura Ceriotti et François Rossi. « Surface Functionalization for Protein and Cell Patterning ». Dans Whole Cell Sensing Systems I, 109–30. Berlin, Heidelberg : Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/10_2009_2.

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Kim, Min Jung, Guk Hwan An et Yong Ho Choa. « Functionalization of Magnetite Nanoparticles for Protein Immobilization ». Dans Solid State Phenomena, 895–98. Stafa : Trans Tech Publications Ltd., 2007. http://dx.doi.org/10.4028/3-908451-31-0.895.

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Palacio-Castañeda, Valentina, Roland Brock et Wouter P. R. Verdurmen. « Generation of Protein-Phosphorodiamidate Morpholino Oligomer Conjugates for Efficient Cellular Delivery via Anthrax Protective Antigen ». Dans Methods in Molecular Biology, 129–41. New York, NY : Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2010-6_8.

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AbstractPhosphorodiamidate morpholino oligomers (PMOs) offer great promise as therapeutic agents for translation blocking or splice modulation due to their high stability and affinity for target sequences. However, in spite of their neutral charge as compared to natural oligonucleotides or phosphorothioate analogs, they still show little permeability for cellular membranes, highlighting the need for effective cytosolic delivery strategies. In addition, the implementation of strategies for efficient cellular targeting is highly desirable to minimize side effects and maximize the drug dose at its site of action. Anthrax toxin is a three-protein toxin of which the pore-forming protein anthrax protective antigen (PA) can be redirected to a receptor of choice and lethal factor (LF), one of the two substrate proteins, can be coupled to various cargoes for efficient cytosolic cargo delivery. In this protocol, we describe the steps to produce the proteins and protein conjugates required for cytosolic delivery of PMOs through the cation-selective pore generated by anthrax protective antigen. The method relies on the introduction of a unique cysteine at the C-terminal end of a truncated LF (aa 1–254), high-yield expression of the (truncated) toxin proteins in E. coli, functionalization of a PMO with a maleimide group and coupling of the maleimide-functionalized PMO to the unique cysteine on LF by maleimide-thiol conjugation chemistry. Through co-administration of PA with LF-PMO conjugates, an efficient cytosolic delivery of PMOs can be obtained.
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Wege, Christina, et Fania Geiger. « Dual Functionalization of Rod-Shaped Viruses on Single Coat Protein Subunits ». Dans Methods in Molecular Biology, 405–24. New York, NY : Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7808-3_27.

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Agrawal, Divya, et Christian P. R. Hackenberger. « Chemoselective Protein Modifications : Methods and Applications for the Functionalization of Viral Capsids ». Dans Chemistry of Organo-Hybrids, 299–348. Hoboken, NJ, USA : John Wiley & Sons, Inc., 2015. http://dx.doi.org/10.1002/9781118870068.ch9.

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Graulus, Geert-Jan, Duy Tien Ta, Huong Tran, Rebekka Hansen, Brecht Billen, Erik Royackers, Jean-Paul Noben et al. « Site-Selective Functionalization of Nanobodies Using Intein-Mediated Protein Ligation for Innovative Bioconjugation ». Dans Methods in Molecular Biology, 117–30. New York, NY : Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9654-4_9.

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Pelaz, Beatriz, Pablo del Pino, Pauline Maffre, Raimo Hartmann, Marta Gallego, Sara Rivera-Fernandez, Jesus M. de la Fuente, G. Ulrich Nienhaus et Wolfgang J. Parak. « Surface Functionalization of Nanoparticles with Polyethylene Glycol : Effects on Protein Adsorption and Cellular Uptake ». Dans Bio-Nano Interfaces, 861–94. New York : Jenny Stanford Publishing, 2024. http://dx.doi.org/10.1201/9781003306498-34.

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Shlyakhtenko, Luda S., Alexander A. Gall et Yuri L. Lyubchenko. « Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy ». Dans Methods in Molecular Biology, 295–312. Totowa, NJ : Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-056-4_14.

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Liu, Yahu A., Zhuo Wang, Weibo Hu, Mingliang Ma, Hui Yang et Ke Wen. « Selected Recent Work on Endo-Functionalization of Cylindrical Macrocyclic Artificial Receptors for Mimicking Protein–Ligand Interactions ». Dans Advanced Materials for Multidisciplinary Applications, 131–53. Cham : Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-39404-1_4.

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Karaca, Banu Taktak, Marketa Hnilova et Candan Tamerler. « Addressable Biological Functionalization of Inorganics : Materials-Selective Fusion Proteins in Bio-nanotechnology ». Dans Bio-Inspired Nanotechnology, 221–55. New York, NY : Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4614-9446-1_8.

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Actes de conférences sur le sujet "Protein functionalization"

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Ismail, Pam. « Plant protein functionalization : Exploring cold plasma ». Dans 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/dyhy9832.

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While plant protein is gaining traction, functionality limitations is hindering its market growth. Improving plant protein functionality will enable successful utilization in various food applications, including meat alternatives. There are several reports on plant protein functionality and applications, but much is still not known about the effect of different processing and modifications on the structural and associated functional changes. Cold plasma, a non-thermal processing technique, is being explored as a novel means for protein functionalization. Cold plasma technology involves the exposure of plasma, a partially ionized gas to proteins. The reactive species, generated by cold plasma can induce several chemical reactions including oxidation, bond cleavage, and/or polymerization. This presentation will demonstrate the effect of various cold plasma treatments on pea and other plant protein structural and functional properties. Protein isolates are subjected to several cold plasma treatment conditions. Reactive species and changes due to potential chemical reactions are monitored. Specifically, changes in the protein tertiary, secondary and primary structure will be evaluated, and chemical reactions will be elucidated. The impact of structural change on protein functionality will be highlighted. This research will provide for the first time a controlled evaluation of the impact of cold plasma on protein structural and functional characteristics. Cold plasma treatment may lead to the production of a viable plant protein ingredient with functional properties that are comparable or better than those of traditional protein ingredients.
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Simion, Monica, Lavinia Ruta, Irina Kleps, Carmen Mihailescu, Teodora Ignat, Dana Stan, Florin Craciunoiu, Mihaela Miu et Adina Bragaru. « Surface Functionalization for Protein Microarray ». Dans 2007 International Semiconductor Conference (CAS 2007). IEEE, 2007. http://dx.doi.org/10.1109/smicnd.2007.4519665.

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Khnouf, Ruba, et Dina Karasneh. « Polydimethyl siloxane microfluidic channel protein functionalization techniques ». Dans 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). IEEE, 2016. http://dx.doi.org/10.1109/nems.2016.7758279.

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Ahmad, Asad, Nathan Gallant, Rasim Guldiken et Onursal Onen. « Surface Functionalization of an Ovarian Cancer Diagnostic Biosensor ». Dans ASME 2011 International Mechanical Engineering Congress and Exposition. ASMEDC, 2011. http://dx.doi.org/10.1115/imece2011-64311.

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Ovarian cancer is the fifth leading cause of death among women in United States and the disease has 1.4% (1 in 71) lifetime risk. Patients with ovarian cancer have a short median survival time after diagnosis with their 5-year survival rate being less than 40%. Early stage ovarian cancer represents an important target for screening since it is lethal in most late stage cases (1). Currently the primary screening procedure for ovarian cancer are blood levels of cancer antigen (CA) 125, however CA 125 levels can also be elevated due to other disorders and do not provide conclusive results (2). Utilizing the research done at the Cell and Molecular Biology department at the University of South Florida which conclusively revealed that urinary levels of bcl-2 are elevated in ovarian cancer patients (3), this research it the first of its kind looking to assess the capture of an analyte protein on a series of potential bioconjugated surfaces for use in a novel acoustic biosensor. Therefore, this research addresses the need for a reliable and economic testing platform to detect ovarian cancer.
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Onen, Onursal, Patricia Kruk et Rasim Guldiken. « Design of Urinary Biomarker Sensor for Early Ovarian Cancer Detection ». Dans ASME 2011 International Mechanical Engineering Congress and Exposition. ASMEDC, 2011. http://dx.doi.org/10.1115/imece2011-62818.

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In this paper, our efforts on the design, surface functionalization and characterization of ultrasonic MEMS sensor for early ovarian cancer is presented. The sensor detects urinary anti-apoptotic protein Bcl-2 level that has been presented as being elevated for different stages of ovarian cancer. Our novel biosensor approach employs a pair of MEMS ultrasound transducers for generating and sensing surface acoustic waves and a delay path in-between with oriented Bcl-2 antibodies (C8C) attached. Piezoelectric surface acoustic wave devices are employed for sensor for their high coupling efficiency and ease of fabrication. The sensor quantifies the cancer progression by detecting mass loading change generated by adhesion of Bcl-2 molecules to antibodies on the sensor surface. The device is fabricated using common MEMS fabrication techniques and a multi-step surface functionalization is utilized for effective protein adhesion. As a result, our biosensor platform has various unique advantages such as: ultra-sensitive (sub pg/ml), low cost, and simple operation (reminiscent of a pregnancy test) not necessitating trained personnel.
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Purqon, Acep, et Nobuyuki Matubayasi. « Free-energy analysis of the preferred configuration of transmembrane protein in model membrane : Roles of lipid and water ». Dans 2ND PADJADJARAN INTERNATIONAL PHYSICS SYMPOSIUM 2015 (PIPS-2015) : Materials Functionalization and Energy Conservations. AIP Publishing LLC, 2016. http://dx.doi.org/10.1063/1.4941862.

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Mar, Mimi N., Buddy D. Ratner, Kyle S. Johnston et Sinclair S. Yee. « Enhanced protein binding on a surface plasmon resonance sensor using a plasma-deposited functionalization film ». Dans Photonics West '95, sous la direction de Joseph R. Lakowicz. SPIE, 1995. http://dx.doi.org/10.1117/12.208520.

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Kengne-Momo, R. P., Y. L. Jeyachandran, A. Assaf, C. Esnault, Ph Daniel, J. F. Pilard, M. J. Durand et al. « Characterization By Raman Spectroscopy Of Gold Surface Functionalization And Immuno-Specific Protein Binding For Biosensor Applications ». Dans XXII INTERNATIONAL CONFERENCE ON RAMAN SPECTROSCOPY. AIP, 2010. http://dx.doi.org/10.1063/1.3482548.

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Bilgili, Hatice Kubra, Gozde Ozaydin Ince, Melis Emanet et Gullu Kiziltas Sendur. « Fabrication of 3D Bone Scaffolds Functionalized With Spatiotemporal Release of BMP-2 Growth Factor via iCVD to Enhance Osteoregeneration ». Dans ASME 2020 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/imece2020-24072.

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Abstract 3D scaffolds are known to be used in bone tissue engineering applications due to their great potential of providing multi-functionalized environment for cells. Different production techniques have been used focusing on changing geometrical features or adding biological/chemical compounds to improve the functionality of current 2D/3D scaffolds. A critical component to this functionalization relates to the effect of endogenous and exogeneous growth factors (GF) in the bone regeneration process that could be incorporated to the scaffolds via Initiated Chemical Vapor Deposition (iCVD) which is a solvent free method that requires low energy while also containing a wide variety of monomer choices for the layer by layer coating of polymers with individual functionality choices. However, GFs come with several difficulties such as rapid deactivation, low protein stability profile and little time of half-life, hence ideal environments that can overcome these issues are yet to be defined. Towards that goal, in this study we develop a computational framework based on the implementation of the advection-diffusion-reaction Partial Differential Equations (PDE) in a Finite Element Analysis (FEA) solver in COMSOL Multiphysics software. The goal is to develop a tool and conduct an initial analysis to be utilized for the simulation of multi-layer scaffold functionalized using encapsulation and immobilization of GFs inside nanoparticles possibly via iCVD. In this paper we focus on the analysis of two typical GF (BMP-2 and TGF) release mechanisms based on the effect of key material and geometrical parameters such as thickness of layers, initial GF concentration, diffusion coefficient, release function and uptake rate (absorption coefficient). The ultimate goal is to develop a model that can be used for future bone scaffold design studies when integrated to more advanced optimization methodologies. This model with further integration and updates of chemical and biological parameter measurements and inclusion of presence of antibodies should lay down a valuable basis for directing possible experimental functionalization efforts and their effects on the healing process of bone tissue. Initial results indicate that the proposed computational model can be utilized to predict the response of multi-layered bone scaffolds in terms of the concentration profiles of the GFs. Results of the parametric study presented in this paper prompt for the relative importance of each parameter in tuning the GF release profiles and point towards the need for formal optimization studies to achieve desired GF release responses considering all factors simultaneously. Among them, the diffusion coefficient is a key parameter with both a dominant effect on the GF profile and its ability to characterize different coatings using iCVD methods. As a next step, the developed framework will be updated to incorporate more detailed surface reactions and morphological data to simulate iCVD coated growth factors and verified with possible in-vitro studies before its integration to a formal optimization methodology.
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Vladu, Alina, Emilia Visileanu, Alina Popescu et Roxana Rodica Constantinescu. « Antimicrobial treatments of undergarments designed for the combat-protective clothing of soldiers ». Dans AHFE 2023 Hawaii Edition. AHFE International, 2023. http://dx.doi.org/10.54941/ahfe1004210.

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Military forces around the world must be equipped with combat-protective clothing made from the best technical textiles available that must provide sufficient protection, increased comfort, and even antimicrobial protection, especially for underwear pieces. Antibacterial treatments for textile materials include the use of various substances such as chitosan, silver, collagen and so on. Chitosan is a polysaccharide that promotes changes in the permeability properties of the membrane wall causing internal osmotic imbalances and consequently inhibits the growth of microorganisms. Silver can also damage the bacterial RNA and DNA, eventually leading to the bacteria`s death. Moreover, collagen, a fibrous natural protein, has an intrinsic ability to fight infection and contributes to keeping the infection site sterile.This paper focuses on the functionalization of four variants of textile materials with different compositions to increase their antibacterial properties. The variants were treated through two different technologies: exhaustion (30 min at 40°C, 500 rpm) and padding (3 consecutive passes). V1-V4 were functionalized with colloidal silver and V1-V3 with a mixture of collagen hydrolysate and colloidal silver through exhaustion. Variants V1-V3 were also treated through the padding technique using 0.5% chitosan, 1% collagen hydrolysate and a mixture of chitosan and colloidal silver. Untreated textile variants were evaluated regarding their physical-mechanical characteristics. Moreover, the functionalized variants were characterised according to their pH, loading degree with active substances (%), wettability by drop test and contact angle methods, thermal resistance (m2K/W) and vapour resistance (m2Pa/W) according to ISO 11092. Treated textile samples were also investigated relating to their antimicrobial resistance using two methods according to ISO 20743/2013 and SR EN ISO 20645/2005. The evaluation of antibacterial resistance using the standards SR EN ISO 20645/2005 and SR EN ISO 20645/2005 demonstrated the effectiveness of treatments with active substances for approx. 95% of the tested variants.
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