Bibliographies thématiques / Postmortem micro-CT

Littérature scientifique sur le sujet « Postmortem micro-CT »

Auteur : Grafiati
Publié le 11 mars 2023

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Articles de revues sur le sujet "Postmortem micro-CT"

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de Crespigny, Alex, Hani Bou-Reslan, Merry C. Nishimura, Heidi Phillips, Richard A. D. Carano et Helen E. D’Arceuil. « 3D micro-CT imaging of the postmortem brain ». Journal of Neuroscience Methods 171, no 2 (juin 2008) : 207–13. http://dx.doi.org/10.1016/j.jneumeth.2008.03.006.

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Dawood, Y., G. J. Strijkers, J. Limpens, R. J. Oostra et B. S. de Bakker. « Novel imaging techniques to study postmortem human fetal anatomy : a systematic review on microfocus-CT and ultra-high-field MRI ». European Radiology 30, no 4 (13 décembre 2019) : 2280–92. http://dx.doi.org/10.1007/s00330-019-06543-8.

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Abstract Background MRI and CT have been extensively used to study fetal anatomy for research and diagnostic purposes, enabling minimally invasive autopsy and giving insight in human fetal development. Novel (contrast-enhanced) microfocus CT (micro-CT) and ultra-high-field (≥ 7.0 T) MRI (UHF-MRI) techniques now enable micron-level resolution that combats the disadvantages of low-field MRI and conventional CT. Thereby, they might be suitable to study fetal anatomy in high detail and, in time, contribute to the postmortem diagnosis of fetal conditions. Objectives (1) To systematically examine the usability of micro-CT and UHF-MRI to study postmortem human fetal anatomy, and (2) to analyze factors that govern success at each step of the specimen preparation and imaging. Method MEDLINE and EMBASE were systematically searched to identify publications on fetal imaging by micro-CT or UHF-MRI. Scanning protocols were summarized and best practices concerning specimen preparation and imaging were enumerated. Results Thirty-two publications reporting on micro-CT and UHF-MRI were included. The majority of the publications focused on imaging organs separately and seven publications focused on whole body imaging, demonstrating the possibility of visualization of small anatomical structures with a resolution well below 100 μm. When imaging soft tissues by micro-CT, the fetus should be stained by immersion in Lugol’s staining solution. Conclusion Micro-CT and UHF-MRI are both excellent imaging techniques to provide detailed images of gross anatomy of human fetuses. The present study offers an overview of the current best practices when using micro-CT and/or UHF-MRI to study fetal anatomy for clinical and research purposes. Key Points • Micro-CT and UHF-MRI can both be used to study postmortem human fetal anatomy for clinical and research purposes. • Micro-CT enables high-resolution imaging of fetal specimens in relatively short scanning time. However, tissue staining using a contrast solution is necessary to enable soft-tissue visualization. • UHF-MRI enables high-resolution imaging of fetal specimens, without the necessity of prior staining, but with the drawback of long scanning time.
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Sandrini, Camilla, Simona Boito, Claudio M. Lombardi et Sophie Lombardi. « Postmortem Micro-CT of Human Fetal Heart—A Systematic Literature Review ». Journal of Clinical Medicine 10, no 20 (15 octobre 2021) : 4726. http://dx.doi.org/10.3390/jcm10204726.

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Micro-computed tomography (CT) is a non-invasive alternative to conventional macroscopic dissection for the evaluation of human fetal cardiac anatomy. This paper aims to systematically review the literature regarding the use of micro-CT to examine human fetal hearts, to illustrate its educational and research implications and to explain its possible directions for the future. A systematic literature review was conducted following the PRISMA statement to identify publications concerning micro-CT applications for the isolated human fetal heart. The search strategy identified nine eligible studies. Micro-CT is technically feasible for postmortem examination of the human fetal heart coming from early and late termination of pregnancy. It reaches high diagnostic accuracy, and it seems to perform better than autopsy in small samples or in the case of early termination of pregnancy. Applications derived from micro-CT allow multiple off-time evaluations and interdisciplinary comparisons for educational purposes and research perspectives in biological and bioengineering domains.
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Lombardi, C. M., V. Zambelli, G. Botta, G. Cattoretti, V. Fesslova, F. Moltrasio, S. Lombardi et S. Cuttin. « OP22.06 : Postmortem micro-computed tomography (micro-CT) of small fetuses and hearts ». Ultrasound in Obstetrics & ; Gynecology 44, S1 (septembre 2014) : 165. http://dx.doi.org/10.1002/uog.13851.

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Lombardi, C. M., V. Zambelli, G. Botta, F. Moltrasio, G. Cattoretti, V. Lucchini, V. Fesslova et M. S. Cuttin. « Postmortem microcomputed tomography (micro-CT) of small fetuses and hearts ». Ultrasound in Obstetrics & ; Gynecology 44, no 5 (13 octobre 2014) : 600–609. http://dx.doi.org/10.1002/uog.13330.

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Foerst, Jason, Timothy Ball et Aaron V. Kaplan. « Postmortem in situ micro-ct evaluation of coronary stent fracture ». Catheterization and Cardiovascular Interventions 76, no 4 (23 février 2010) : 527–31. http://dx.doi.org/10.1002/ccd.22498.

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Shelmerdine, S. C., M. Singh, I. C. Simcock, A. D. Calder, M. Ashworth, A. Beleza, N. J. Sebire et O. J. Arthurs. « Characterization of Bardet–Biedl syndrome by postmortem microfocus computed tomography (micro‐CT) ». Ultrasound in Obstetrics & ; Gynecology 53, no 1 (janvier 2019) : 132–34. http://dx.doi.org/10.1002/uog.19190.

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Vande Velde, Greetje, Ellen De Langhe, Jennifer Poelmans, Peter Bruyndonckx, Emiliano d'Agostino, Erik Verbeken, Ria Bogaerts, Rik Lories et Uwe Himmelreich. « Longitudinal in vivo microcomputed tomography of mouse lungs : No evidence for radiotoxicity ». American Journal of Physiology-Lung Cellular and Molecular Physiology 309, no 3 (1 août 2015) : L271—L279. http://dx.doi.org/10.1152/ajplung.00098.2015.

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Before microcomputed tomography (micro-CT) can be exploited to its full potential for longitudinal monitoring of transgenic and experimental mouse models of lung diseases, radiotoxic side effects such as inflammation or fibrosis must be considered. We evaluated dose and potential radiotoxicity to the lungs for long-term respiratory-gated high-resolution micro-CT protocols. Free-breathing C57Bl/6 mice underwent four different retrospectively respiratory gated micro-CT imaging schedules of repeated scans during 5 or 12 wk, followed by ex vivo micro-CT and detailed histological and biochemical assessment of lung damage. Radiation exposure, dose, and absorbed dose were determined by ionization chamber, thermoluminescent dosimeter measurements and Monte Carlo calculations. Despite the relatively large radiation dose delivered per micro-CT acquisition, mice did not show any signs of radiation-induced lung damage or fibrosis when scanned weekly during 5 and up to 12 wk. Doubling the scanning frequency and once tripling the radiation dose as to mimic the instant repetition of a failed scan also stayed without detectable toxicity after 5 wk of scanning. Histological analyses confirmed the absence of radiotoxic damage to the lungs, thereby demonstrating that long-term monitoring of mouse lungs using high-resolution micro-CT is safe. This opens perspectives for longitudinal monitoring of (transgenic) mouse models of lung diseases and therapeutic response on an individual basis with high spatial and temporal resolution, without concerns for radiation toxicity that could potentially influence the readout of micro-CT-derived lung biomarkers. This work further supports the introduction of micro-CT for routine use in the preclinical pulmonary research field where postmortem histological approaches are still the gold standard.
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Tekus, Eva, Alexandra Miko, Nora Furedi, Ildiko Rostas, Judit Tenk, Tamas Kiss, Istvan Szitter et al. « Body fat of rats of different age groups and nutritional states : assessment by micro-CT and skinfold thickness ». Journal of Applied Physiology 124, no 2 (1 février 2018) : 268–75. http://dx.doi.org/10.1152/japplphysiol.00884.2016.

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Obesity presents a growing public health problem. Therefore the analysis of body composition is important in clinical practice as well as in animal research models of obesity; hence precise methods for the assessment of body fat would be essential. We aimed to evaluate in vivo abdominal microcomputed tomography scan restricted to the L1–L3 region [micro-CT(L1–L3)], a skinfold thickness-based method (STM), and postmortem body composition analysis (PMA) with regard to whole body micro-CT scan in rats. Male Wistar rats of different age groups (from 3 to 24 mo) and nutritional states (normally fed, high-fat diet-induced obese, and calorie-restricted) were used. The fat percentage was determined with micro-CT(L1–L3) and whole body scan in anesthetized rats. Their skinfold thickness was measured in five locations with a Lange caliper. Wet weights of epididymal and retroperitoneal fat pads were determined via PMA. With regard to fat mass, the strongest correlation was observed between abdominal and whole body micro-CT. The other methods showed weaker associations with whole body micro-CT and with each other. Micro-CT(L1–L3) and PMA showed similar age-associated increase in fat mass between 3 and 18 mo. Micro-CT(L1–L3), STM, and PMA were efficient to detect differences in fat mass values in groups of different nutritional states. Micro-CT(L1–L3) appears to be a useful method for body fat assessment in rats with reduced scanning time. In rats, STM may also be a useful, low priced, noninvasive, and simple in vivo technique to assess obesity. NEW & NOTEWORTHY Body fat of rats assessed by in vivo abdominal microcomputed tomography of the L1–L3 region strongly correlates with values determined by whole body scan. Therefore, it is a useful method for fat assessment with reduced scanning time. Skinfold thickness measurement is an in vivo technique to assess progression of obesity in rats.
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Lupariello, Francesco, Tullio Genova, Federico Mussano, Giancarlo Di Vella et Giovanni Botta. « Micro-CT to study and reconstruct fetal and infant coronary arteries : a pilot study on a novel post-mortem technique ». European Journal of Anatomy 26, no 4 (juillet 2022) : 443–47. http://dx.doi.org/10.52083/fcws3396.

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For a significant part of infant and fetal deaths, specific pathophysiologic processes cannot be recognized. Thus, the scientific community is called to identify novel post-mortem diagnostic tools. This manuscript proposes the results of a pilot study which reports a novel post-mortem technique to study and reconstruct fetal/ infant coronary arteries. The study included human fetuses characterized by the absence of macroscopic cardiac abnormalities at postmortem in situ examination. For the study of fetal hearts, it was used a curing radiopaque silicone rubber compound (which solidified after injection in the coronary arteries) and an X-Ray microtomography (micro-CT). After micro- CT scans, coronary arteries’ branches were reconstructed throughout a specific software. At injection, it was possible to macroscopically evaluate coronary arteries’ perfusion. The analysis of the three-dimensional reconstructions highlighted that the aforementioned compound reached deep branches too. This approach can be considered a novel post-mortem technique for fetal/infant hearts. Nevertheless, the manuscript also discussed the following limitations: in some spots, coronary arteries’ reconstruction appeared interrupted; the compound also perfused parts of internal cardiac chambers. Until now, in the literature there are not methods that allow study with reconstruction of fetal/infant coronary arteries throughout micro-CT. The present paper pointed out the first indications for the application of this technique in human samples.
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Actes de conférences sur le sujet "Postmortem micro-CT"

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RAPKING, DANIEL, LUKE GEISE, ROBERT WHEELER et MARK FLORES. « DEVELOPMENT OF SINGLE CASE STUDIES FOR MICROMECHANICS DAMAGE EVOLUTION IN UNIDIRECTIONAL COMPOSITES ». Dans Proceedings for the American Society for Composites-Thirty Seventh Technical Conference. Destech Publications, Inc., 2022. http://dx.doi.org/10.12783/asc37/36433.

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This study continues to further expand the methods utilized by the Air Force Research Laboratory (AFRL) to understand microscale damage evolution. The microscale test data collected here will be used to validate many multiscale modeling frameworks’ ability to accurately predict damage evolution in complex, heterogeneous microscale structures. Previous work has conducted testing of transverse compression (TC) pillars in scanning electron microscope (SEM) load stages allowing for the measurement of surface level strains while being loaded. The limitation of this approach is a lack of understanding how damage develops through the thickness of the pillar during loading. This work involved the fabrication of a transverse compression micropillar and the experimental test of this sample that visualized through thickness damage evolution in-situ. A post mortem tomography was conducted to fully analyze damage evolution in the micro-pillar to validate the planned framework would be viable for additional microstructural geometries. A complete examination of the pillar, a postmortem Nano-CT, and a preliminary methodology for evaluating multiscale models are presented within this work.
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