Littérature scientifique sur le sujet « Pathogen enrichment »
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Articles de revues sur le sujet "Pathogen enrichment"
MURAKAMI, TAKU. « Filter-Based Pathogen Enrichment Technology for Detection of Multiple Viable Foodborne Pathogens in 1 Day ». Journal of Food Protection 75, no 9 (1 septembre 2012) : 1603–10. http://dx.doi.org/10.4315/0362-028x.jfp-12-039.
Texte intégralLee, Justin S., Ryan S. Mackie, Thomas Harrison, Basir Shariat, Trey Kind, Timo Kehl, Martin Löchelt, Christina Boucher et Sue VandeWoude. « Targeted Enrichment for Pathogen Detection and Characterization in Three Felid Species ». Journal of Clinical Microbiology 55, no 6 (22 mars 2017) : 1658–70. http://dx.doi.org/10.1128/jcm.01463-16.
Texte intégralHUANG, SHISHI, TAY BOON HUI, HYUN-GYUN YUK et QIANWANG ZHENG. « Development of an Effective Two-Step Enrichment Process to Enhance Bax System Detection of Healthy and Injured Salmonella Enteritidis in Liquid Whole Egg and Egg Yolk ». Journal of Food Protection 83, no 3 (14 février 2020) : 397–404. http://dx.doi.org/10.4315/0362-028x.jfp-19-280.
Texte intégralKim, Hyochin, et Arun K. Bhunia. « SEL, a Selective Enrichment Broth for Simultaneous Growth of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes ». Applied and Environmental Microbiology 74, no 15 (6 juin 2008) : 4853–66. http://dx.doi.org/10.1128/aem.02756-07.
Texte intégralFurtwängler, Anja, Judith Neukamm, Lisa Böhme, Ella Reiter, Melanie Vollstedt, Natasha Arora, Pushpendra Singh et al. « Comparison of target enrichment strategies for ancient pathogen DNA ». BioTechniques 69, no 6 (décembre 2020) : 455–59. http://dx.doi.org/10.2144/btn-2020-0100.
Texte intégralZHAO, TONG, et MICHAEL P. DOYLE. « Evaluation of Universal Preenrichment Broth for Growth of Heat-Injured Pathogens ». Journal of Food Protection 64, no 11 (1 novembre 2001) : 1751–55. http://dx.doi.org/10.4315/0362-028x-64.11.1751.
Texte intégralParichehr, Moezi, Kargar Mohammad, Doosti Abbas et Khoshneviszadeh Mehdi. « Developing a multiplex real-time PCR with a new pre-enrichment to simultaneously detect four foodborne bacteria in milk ». Future Microbiology 14, no 10 (juillet 2019) : 885–98. http://dx.doi.org/10.2217/fmb-2019-0044.
Texte intégralHayashi, Masahiro, Tatsuya Natori, Sayoko Kubota-Hayashi, Machiko Miyata, Kiyofumi Ohkusu, Keiko Kawamoto, Hisao Kurazono, Souichi Makino et Takayuki Ezaki. « A New Protocol to Detect Multiple Foodborne Pathogens with PCR Dipstick DNA Chromatography after a Six-Hour Enrichment Culture in a Broad-Range Food Pathogen Enrichment Broth ». BioMed Research International 2013 (2013) : 1–10. http://dx.doi.org/10.1155/2013/295050.
Texte intégralTORTORELLO, M. L., K. F. REINEKE, D. S. STEWART et R. B. RAYBOURNE. « Comparison of Methods for Determining the Presence of Escherichia coli O157:H7 in Apple Juice ». Journal of Food Protection 61, no 11 (1 novembre 1998) : 1425–30. http://dx.doi.org/10.4315/0362-028x-61.11.1425.
Texte intégralHahm, Byoung-Kwon, Hyochin Kim, Atul K. Singh et Arun K. Bhunia. « Pathogen enrichment device (PED) enables one-step growth, enrichment and separation of pathogen from food matrices for detection using bioanalytical platforms ». Journal of Microbiological Methods 117 (octobre 2015) : 64–73. http://dx.doi.org/10.1016/j.mimet.2015.07.016.
Texte intégralThèses sur le sujet "Pathogen enrichment"
Bernardo, Letizia. « IDENTIFICATION AND CHARACTERIZATION OF PROTEINS INVOLVED IN BIOTIC STRESS RESISTANCE OF CEREALS ». Doctoral thesis, Università degli studi di Padova, 2010. http://hdl.handle.net/11577/3426966.
Texte intégralLa ruggine fogliare è una delle malattie più importanti della coltura dell'orzo (Hordeum vulgare) ed è causata dal patogeno fungino biotrofo Puccinia hordei. Il fungo penetra attraverso gli stomi delle foglie dell’orzo e colonizza le cellule del mesofillo, crescendo poi per via sistemica nei tessuti vascolari della foglia. Il gene Rph15 di orzo è di considerevole importanza per il miglioramento genetico della resistenza in quanto conferisce resistenza a più di 350 isolati di P. hordei provenienti da tutto il mondo (Weerasena et al. 2004). L’interazione pianta-patogeno attiva numerosi processi di signalling cellulare e, molto probabilmente, l’accumulo delle proteine e i cambiamenti nel pattern di fosforilazione delle proteine giocano un ruolo centrale nella risposta della pianta in seguito a stress biotico. In questo lavoro, un approccio di tipo proteomico è stato intrapreso per studiare i cambiamenti nei pattern proteici totali e delle proteine fosforilate in seguito a risposta alla ruggine fogliare in due linee quasi isogeniche di orzo, Bowman e la linea Rph15, che differiscono per l’ introgressione del gene Rph15. Due tempi di infezione, 24 ore e quattro giorni, sono stati presi in considerazione per le analisi. Nessuna differenza statisticamente significativa è stata individuate nel primo tempo di infezione precoce, a 24 ore dopo l’inoculo, sia per quanto riguarda le proteine totali che per le proteine fosforilate. A 4 giorni dall’infezione, l’ analisi delle proteine totali ha consentito di identificare ventuno spot proteici significativamente up o down regolati in risposta all’ infezione con un fold-change almeno di 2. La maggior parte delle proteine down-regolate sono state trovate nel campione infettato della linea isogenica contenente il gene di resistenza Rph15, mentre non è stata riscontrata alcuna differenza statisticamente significativa nel pattern proteico della linea isogenica suscettibile. Diciannove dei 21 spot proteici sono stati caratterizzati mediante analisi LC-MS/MS e identificati essere implicati in processi come fotosintesi, metabolismo degli zuccheri, bilancio energetico e risposte di difesa. L’analisi del fosfoproteoma è stata condotta a quattro giorni dopo l’inoculo. Una tecnica di arricchimento in fosfoproteine basata su MOAC (cromatografia di affinità mediante ossidi metallici) che è stata ottimizzata per la successiva analisi 2DE.
Jani, Mehul. « Genomic Island Discovery through Enrichment of Statistical Modeling with Biological Information ». Thesis, University of North Texas, 2018. https://digital.library.unt.edu/ark:/67531/metadc1248417/.
Texte intégralPenczykowski, Rachel M. « Interactions between ecosystems and disease in the plankton of freshwater lakes ». Diss., Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/50368.
Texte intégralTrindade, Maria Theresa. « Detection of pathogenic bacteria and fecal enterococci in recreational water with an evanescent wave fiber optic biosensor ». [Tampa, Fla] : University of South Florida, 2005. http://purl.fcla.edu/usf/dc/et/SFE0001443.
Texte intégralPanji, Sumir. « Identification of bacterial pathogenic gene classes subject to diversifying selection ». Thesis, University of the Western Cape, 2009. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_5842_1297942831.
Texte intégralAvailability of genome sequences for numerous bacterial species comprising of different bacterial strains allows elucidation of species and strain specific adaptations that facilitate their survival in widely fluctuating micro-environments and enhance their pathogenic potential. Different bacterial species use different strategies in their pathogenesis and the pathogenic potential of a bacterial species is dependent on its genomic complement of virulence factors. A bacterial virulence factor, within the context of this study, is defined as any endogenous protein product encoded by a gene that aids in the adhesion, invasion, colonization, persistence and pathogenesis of a bacterium within a host. Anecdotal evidence suggests that bacterial virulence genes are undergoing diversifying evolution to counteract the rapid adaptability of its host&rsquo
s immune defences. Genome sequences of pathogenic bacterial species and strains provide unique opportunities to study the action of diversifying selection operating on different classes of bacterial genes.
Nyarko, Esmond Boafo. « Improved Recovery And Rapid Identification Of Strains, Mixed Strains, Mixed Species, And Various Physiological States Of Foodborne Pathogens Using Infrared Spectroscopy ». ScholarWorks @ UVM, 2014. http://scholarworks.uvm.edu/graddis/276.
Texte intégralMarimuthu, Saranya. « Development of quantitative RNA sequencing methods to understand RNA variant diversity ». Thesis, 2021. https://etd.iisc.ac.in/handle/2005/5548.
Texte intégralLin, Chien-Ju, et 林建汝. « Feasibility Study of Rapid Detection for Foodborne Pathogenic Bacterial by Multiple primers-PCR and PCR-enrichment ». Thesis, 2007. http://ndltd.ncl.edu.tw/handle/10614801838677193718.
Texte intégral國立臺灣海洋大學
食品科學系
95
First part of this study is to develop a combination of multiple primers for the major food poisoning pathogens: Bacillus cereus、Campylobacter jejuni, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella spp., Shigella spp., Staphylococcus aureus and Vibrio parahaemolyticus, and further to investigate the feasibility of applying those primer combination for rapid detection the presence of those pathogens by PCR. The second part of this study is to develop a PCR enrichment technique that can rapidly identify the presence of target pathogen at low number of cells and at a shorter period of incubation time as compared to traditional enrichment procedures. Escherichia coli O157:H7, and Vibrio parahaemolyticus were selected for this part of study. Results indicated that the production of non-specific PCR products will obstruct the interpretation of DNA electrophoretograms with multiple PCR primer combination, and thus make individual identification of those pathogens impossible. Possible approaches to resolve these inferences including categorized primer pair and combination design, Ta (annealing temperature) selection, and target pathogen grouping were suggested for further studies. In PCR enrichment experiment, results suggested that the detection limit can be reduced from 102~103 CFU/ml for traditional selective enrichment procedures to as low as a single cell for both Escherichia coli O157:H7 and Vibrio parahaemolyticus by PCR enrichment technique. Since PCR enrichment technique tends to cause non-specific signal and different primers need respective Ta, it is not suitable for applying on various pathogenic microbes. However, PCR enrichment technique will still be a potential, low costing, time saving, and sensitive methology while the problems of annealing conditions and non-specific PCR reactions are solved.
Sousa, Ana Catarina da Silva e. « Development of rapid methods for the detection of pathogenic microorganisms, based on NAM-FISH technology ». Master's thesis, 2018. http://hdl.handle.net/10773/25528.
Texte intégralO acesso de microrganismos patogénicos ao corpo humano pode comprometer a saúde do indivíduo, provocando variadas manifestações clínicas. Helicobacter pylori e Campylobacter são dois importantes patógenos gastrointestinais. A infeção por H. pylori é uma das infeções humanas mais comuns, cujo tratamento inclui a administração de antibióticos, nomeadamente fluoroquinolonas (FQ). Contudo, tem-se verificado um aumento da resistência de H. pylori a FQ, o que pode resultar em falhas no tratamento, tornando-se importante não só a deteção da bactéria, mas também a definição do seu perfil de resistência. Campylobacter é atualmente considerada a principal causa de doenças transmitidas por alimentos, encontrando-se normalmente associada ao consumo de carne crua. A deteção de microrganismos patogénicos pode ser alcançada por técnicas de cultura convencionais ou por métodos moleculares, nomeadamente testes imunológicos ou de deteção de ácidos nucleicos. A Biomode é uma empresa inovadora que desenvolve e comercializa métodos de diagnóstico rápidos baseados na tecnologia de hibridação fluorescente in situ (FISH) de ácidos nucleicos mímicos (NAM), NAM-FISH, que possibilita a deteção rápida de microrganismos, através da hibridação de sondas fluorescentes complementares com sequências específicas presentes no microrganismo alvo. Neste contexto, o presente trabalho teve como foco duas aplicações do NAM-FISH. Na área clínica, o objetivo principal foi o desenvolvimento de um método para a deteção de H. pylori e da resistência a FQ. Para tal, procedeu-se ao desenho de sondas de Peptide Nucleic Acid (PNA) e Locked Nucleic Acid (LNA)/2’OMe para a deteção de mutações causadoras da resistência. De forma a cobrir as mutações mais prevalentes, bem como o fenótipo wild-type, foram selecionadas 5 sondas de LNA/2’OMe. Na área da segurança alimentar, foi objetivo a otimização de um método de PNA-FISH para a deteção de Campylobacter em amostras alimentares. Um ensaio preliminar da inclusividade/exclusividade da sonda Campylobacter resultou na deteção de dois microrganismos não-alvo, H. cinaedi e H. pamatensis. Para a otimização do procedimento, foram utilizadas amostras de carne de frango crua, inoculadas artificialmente com C. jejuni. Antes do PNAFISH, foi introduzido um novo passo, no qual as amostras enriquecidas são sujeitas a uma centrifugação (10 000 g), seguida de ressuspensão em 0.1% Triton X-100, com o objetivo de reduzir a autofluorescência forte visualizada em amostras sem qualquer tratamento. Testou-se também a possibilidade de um enriquecimento das amostras em dois passos, no entanto, esta abordagem não demonstrou vantagens comparativamente ao procedimento em um passo. Efetuou-se ainda um teste de robustez, requerido pela AOAC International para a obtenção de certificação de produto, que revelou que a variação dos parâmetros do tempo e temperatura de hibridação influenciam a performance do método, pelo que as condições do PNA-FISH devem ser rigorosamente controladas. Os resultados obtidos neste estudo mostraram que o método PNAFISH é adequado para a rápida deteção de Campylobacter em amostras alimentares. Com este trabalho conclui-se que, embora os dois métodos baseados em NAM-FISH sejam uma opção promissora para a deteção de H. pylori e Campylobacter, ambos necessitam de otimização futura.
Mestrado em Biotecnologia
Wilson, TK. « Development of a streamlined, selective-enrichment culture, one-tube (RT-)PCR-enzyme hybridization assay to detect bacterial fish pathogens in covertly infected farmed salmonids ». Thesis, 2003. https://eprints.utas.edu.au/22118/1/whole_WilsonTeresaKaye2003_thesis.pdf.
Texte intégralLivres sur le sujet "Pathogen enrichment"
McKinney, Mark William. The application of "deletion enrichment" strategy for the isolation of DNA sequences unique to pathogenic clostridia. [s.l : The Author], 1991.
Trouver le texte intégralChapitres de livres sur le sujet "Pathogen enrichment"
Prasad, Priya. « Loss of Function of Sth1, The Catalytic Component of RSC (Remodel the Structure of Chromatin) Complex Grossly Alter the Chromatin Architecture ». Dans Proceedings of the Conference BioSangam 2022 : Emerging Trends in Biotechnology (BIOSANGAM 2022), 168–75. Dordrecht : Atlantis Press International BV, 2022. http://dx.doi.org/10.2991/978-94-6463-020-6_17.
Texte intégralPenyalver, R., E. Bertolini, A. Olmos, A. García, M. Cambra et M. M. López. « Detection of Pseudomonas savastanoi pv. savastanoi (Pss) on Asymptomatic Olive Plant Tissues by Enrichment-PCR ». Dans Plant Pathogenic Bacteria, 421–24. Dordrecht : Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-010-0003-1_94.
Texte intégralKinoshita, Eiji, Emiko Kinoshita-Kikuta et Tohru Koike. « Phos-tag-Based Affinity Chromatography Techniques for Enrichment of the Phosphoproteome ». Dans Protein Modifications in Pathogenic Dysregulation of Signaling, 17–30. Tokyo : Springer Japan, 2015. http://dx.doi.org/10.1007/978-4-431-55561-2_2.
Texte intégralVan Vuurde, J. W., M. A. Ruissen et H. Vruggink. « Principles and Prospects of New Serological Techniques Including Immunosorbent Immunofluorescence, Immunoaffinity Isolation and Immunosorbent Enrichment for Sensitive Detection of Phytopathogenic Bacteria ». Dans Plant Pathogenic Bacteria, 835–42. Dordrecht : Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3555-6_180.
Texte intégralShen, Cangliang, et Yifan Zhang. « Isolation of Foodborne Pathogens on Selective, Differential, and Enrichment Medium by Streak-Plating ». Dans Food Microbiology Laboratory for the Food Science Student, 25–30. Cham : Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-26197-8_4.
Texte intégralLópez, M. M., M. T. Gorris, P. Llop, J. Cubero, B. Vicedo et M. Cambra. « Selective Enrichment Improves Isolation, Serological and Molecular Detection of Plant Pathogenic Bacteria ». Dans Developments in Plant Pathology, 117–21. Dordrecht : Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-009-0043-1_25.
Texte intégralSpeicher, David J., Jalees A. Nasir, Peng Zhou et Danielle E. Anderson. « Whole-Genome Sequencing of Pathogens in : A Target-Enrichment Approach for SARS-CoV-2 ». Dans Methods in Molecular Biology, 119–37. New York, NY : Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1518-8_8.
Texte intégralArunachalam, Kannappan, et Chunlei Shi. « Developments in rapid detection/high throughput screening techniques for identifying pathogens in food ». Dans Advances in ensuring the microbiological safety of fresh produce, 97–136. Burleigh Dodds Science Publishing, 2023. http://dx.doi.org/10.19103/as.2023.0121.08.
Texte intégralBlackburn, Clive de W. « ENRICHMENT SEROLOGY | An Enhanced Cultural Technique for Detection of Food-borne Pathogens ». Dans Encyclopedia of Food Microbiology, 589–97. Elsevier, 1999. http://dx.doi.org/10.1006/rwfm.1999.0500.
Texte intégralSingh, Karuna, et Radha Chaube. « Enrichment of drug resistance genes in human pathogenic bacteria showing antimicrobial resistance ». Dans Antimicrobial Resistance in Wastewater and Human Health, 41–60. Elsevier, 2023. http://dx.doi.org/10.1016/b978-0-323-96124-0.00008-8.
Texte intégralActes de conférences sur le sujet "Pathogen enrichment"
Manzanas, Carlos, Xiao Jiang, John A. Lednicky et Z. Hugh Fan. « Development of Ball-Enabled Miniaturized Valves for Sample Preparation and Microheaters for Pathogen Detection ». Dans ASME 2020 Fluids Engineering Division Summer Meeting collocated with the ASME 2020 Heat Transfer Summer Conference and the ASME 2020 18th International Conference on Nanochannels, Microchannels, and Minichannels. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/fedsm2020-20379.
Texte intégralLopes, Camila Galvão, Gabriele Campos, Jaqueline Wang, Ana Cristina Girardi et Maria Rita Passos Bueno. « Characterization of de novo variants in exomes of individuals with autism spectrum disorder ». Dans XIV Congresso Paulista de Neurologia. Zeppelini Editorial e Comunicação, 2023. http://dx.doi.org/10.5327/1516-3180.141s1.564.
Texte intégralBowman, Andrew, Andrew Mack, Wondwossen A. Gebreyes et Julie A. Funk. « Comparison of enrichment schemes for the isolation of Yersinia enterocolitica ». Dans Fifth International Symposium on the Epidemiology and Control of Foodborn Pathogens in Pork. Iowa State University, Digital Press, 2003. http://dx.doi.org/10.31274/safepork-180809-534.
Texte intégralDavies, H., Christine E. R. Dodd, S. Tötermeyer, J. Wiseman et Ken H. Mellits. « A rapid, sensitive enrichment PCR to detect Salmonella and ETEC infections in pigs ». Dans Eighth International Symposium on the Epidemiology and Control of Foodborne Pathogens in Pork. Iowa State University, Digital Press, 2009. http://dx.doi.org/10.31274/safepork-180809-825.
Texte intégralBernate, Ilze, et Martins Sabovics. « Research on germinated wheat grain, broccoli, alfalfa, radish and hemp seeds microbiological safety ». Dans Research for Rural Development 2021 : annual 27th International scientific conference proceedings. Latvia University of Life Sciences and Technologies, 2021. http://dx.doi.org/10.22616/rrd.27.2021.013.
Texte intégralMalorny, Burkhard, Nadine Krämer, Håkan Vigre, Jeffrey Hoorfar et Charlotta Löfström. « A novel strategy to obtain quantitative data for modelling : Combined enrichment and real-time PCR for enumeration of salmonellae from pig carcasses ». Dans Fifth International Symposium on the Epidemiology and Control of Foodborn Pathogens in Pork. Iowa State University, Digital Press, 2011. http://dx.doi.org/10.31274/safepork-180809-578.
Texte intégralNollet, Nathalie, Dominiek Maes, Lieven De Zutter, Aart de Kruif et Jan van Hoof. « Evaluation of different enrichment media for the isolation of Salmonella spp from faeces and lymph nodes in slaughter pigs ». Dans Fourth International Symposium on the Epidemiology and Control of Salmonella and Other Food Borne Pathogens in Pork. Iowa State University, Digital Press, 2001. http://dx.doi.org/10.31274/safepork-180809-1183.
Texte intégralGiannakou, Lydia, Athanasios-Stefanos Giannopoulos, Erasmia Rouka, Chrissi Hatzoglou, Konstantinos Gourgoulianis et Sotirios Zarogiannis. « Investigation and functional enrichment analysis of the human gene interaction network with common gram-negative respiratory pathogens predicts possible association with lung adenocarcinoma ». Dans ERS International Congress 2019 abstracts. European Respiratory Society, 2019. http://dx.doi.org/10.1183/13993003.congress-2019.pa5417.
Texte intégralRapports d'organisations sur le sujet "Pathogen enrichment"
VanderGheynst, Jean, Michael Raviv, Jim Stapleton et Dror Minz. Effect of Combined Solarization and in Solum Compost Decomposition on Soil Health. United States Department of Agriculture, octobre 2013. http://dx.doi.org/10.32747/2013.7594388.bard.
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