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Articles de revues sur le sujet « OsSULTR »

Auteur : Grafiati
Publié le 9 mars 2023
Mis à jour le 10 mars 2023

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1

Wang, Gaopeng, Yue Wu, Li Ma, Yan Lin, Yuxiang Hu, Mengzhu Li, Weiwei Li, Yanfeng Ding et Lin Chen. « Phloem loading in rice leaves depends strongly on the apoplastic pathway ». Journal of Experimental Botany 72, no 10 (24 février 2021) : 3723–38. http://dx.doi.org/10.1093/jxb/erab085.

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Abstract Phloem loading is the first step in sucrose transport from source leaves to sink organs. The phloem loading strategy in rice remains unclear. To determine the potential phloem loading mechanism in rice, yeast invertase (INV) was overexpressed by a 35S promoter specifically in the cell wall to block sugar transmembrane loading in rice. The transgenic lines exhibited obvious phloem loading suppression characteristics accompanied by the accumulation of sucrose and starch, restricted vegetative growth and decreased grain yields. The decreased sucrose exudation rate with p-chloromercuribenzenesulfonic acid (PCMBS) treatment also indicated that rice actively transported sucrose into the phloem. OsSUT1 (SUCROSE TRANSPORTER 1) showed the highest mRNA levels of the plasma membrane-localized OsSUTs in source leaves. Cross sections of the OsSUT::GUS transgenic plants showed that the expression of OsSUT1 and OsSUT5 occurred in the phloem companion cells. Rice ossut1 mutants showed reduced growth and grain yield, supporting the hypothesis of OsSUT1 acting in phloem loading. Based on these results, we conclude that apoplastic phloem loading plays a major role in the export of sugar from rice leaves.
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Réthoré, Elise, Nusrat Ali, Jean-Claude Yvin et Seyed Abdollah Hosseini. « Silicon Regulates Source to Sink Metabolic Homeostasis and Promotes Growth of Rice Plants under Sulfur Deficiency ». International Journal of Molecular Sciences 21, no 10 (23 mai 2020) : 3677. http://dx.doi.org/10.3390/ijms21103677.

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Being an essential macroelement, sulfur (S) is pivotal for plant growth and development, and acute deficiency in this element leads to yield penalty. Since the last decade, strong evidence has reported the regulatory function of silicon (Si) in mitigating plant nutrient deficiency due to its significant diverse benefits on plant growth. However, the role of Si application in alleviating the negative impact of S deficiency is still obscure. In the present study, an attempt was undertaken to decipher the role of Si application on the metabolism of rice plants under S deficiency. The results showed a distinct transcriptomic and metabolic regulation in rice plants treated with Si under both short and long-term S deficiencies. The expression of Si transporters OsLsi1 and OsLsi2 was reduced under long-term deficiency, and the decrease was more pronounced when Si was provided. The expression of OsLsi6, which is involved in xylem loading of Si to shoots, was decreased under short-term S stress and remained unchanged in response to long-term stress. Moreover, the expression of S transporters OsSULTR tended to decrease by Si supply under short-term S deficiency but not under prolonged S stress. Si supply also reduced the level of almost all the metabolites in shoots of S-deficient plants, while it increased their level in the roots. The levels of stress-responsive hormones ABA, SA, and JA-lle were also decreased in shoots by Si application. Overall, our finding reveals the regulatory role of Si in modulating the metabolic homeostasis under S-deficient condition.
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Sun, Y., A. Reinders, K. R. LaFleur, T. Mori et J. M. Ward. « Transport Activity of Rice Sucrose Transporters OsSUT1 and OsSUT5 ». Plant and Cell Physiology 51, no 1 (3 décembre 2009) : 114–22. http://dx.doi.org/10.1093/pcp/pcp172.

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Scofield, Graham N., Tatsuro Hirose, Judy A. Gaudron, Robert T. Furbank, Narayana M. Upadhyaya et Ryu Ohsugi. « Antisense suppression of the rice transporter gene, OsSUT1, leads to impaired grain filling and germination but does not affect photosynthesis ». Functional Plant Biology 29, no 7 (2002) : 815. http://dx.doi.org/10.1071/pp01204.

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OsSUT1 encodes a rice sucrose transport protein that is highly expressed in developing grain, leaf sheath and stem after heading, and in germinating seedlings, but only at very low levels in source leaves. In this study, we have used antisense gene suppression to elucidate the in vivo function of OsSUT1. Rice was transformed with an antisense construct containing a portion of the 3′-coding and non-coding regions of OsSUT1 driven by the maize ubiquitin-1 promoter. Twenty-six independent stably transformed lines were obtained. T0 and selfed T1 progeny were analysed for suppression of OsSUT1 expression and function. Many of the plants showed a significant reduction in their ability to produce filled grain, and final grain weight was reduced. Severe phenotypes correlated with a reduction in OsSUT1 transcript level in filling grain. Unlike SUT1 antisense suppression in dicots, source supply of photosynthate was unaffected in these transformants. This provides the first direct evidence for the requirement of a sucrose transporter for grain filling in a cereal species. Furthermore, seed from some of the T0 population showed a reduction in the rate of germination and growth, supporting the hypothesis that OsSUT1 may also play a role in transporting sucrose remobilized from starch reserves in germinating seeds.
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Furbank, Robert T., Graham N. Scofield, Tatsuro Hirose, Xin-Ding Wang, John W. Patrick et Christina E. Offler. « Cellular localisation and function of a sucrose transporter OsSUT1 in developing rice grains ». Functional Plant Biology 28, no 12 (2001) : 1187. http://dx.doi.org/10.1071/pp01111.

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We previously reported the cloning and tissue-specific expression of a gene encoding a sucrose/proton symporter in rice (Oryza sativa L.), OsSUT1 (Hirose et al. 1997, Plant Cell Physiology38, 1389–1396). This gene is expressed at high levels in the filling grain, leaf sheath and stem. Expression in these tissues occurred only after heading i.e. during the development of the reproductive structure as a major sink. In this paper, we report localisation of the transcript and protein to specific cells in the filling rice grain by in situ hybridisation with a probe from theOsSUT1 cDNA, and immunolocalisation of OsSUT1 and proton-pumping ATPase (H+-ATPase). An OsSUT1 cDNA probe recognises a transcript of approximately 2.4 kb, and the SUT1 antibody recognises a protein of approximately 55 kDa in total membrane protein extracts from the filling grain, leaf sheath and stem. In the developing grain, OsSUT1 is expressed at low levels before heading, with expression reaching a peak approximately ten days after emergence of the panicle from the sheath. Transcript is then present throughout seed development, with expression falling substantially after about 25 days post-heading. Both transcript and protein are localised to the aleurone cells of the developing grain, and are also detected in the maternal tissue, particularly the nucellus, vascular parenchyma tissue and the nucellar projection. Tissue slices from filling rice grain showed high rates of sucrose uptake that were inhibited by pCMBS. The role of OsSUT1 in sucrose transport to the filling grain endosperm is discussed.
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Li, Dandan, Rucong Xu, Dong Lv, Chunlong Zhang, Hong Yang, Jianbo Zhang, Jiancheng Wen, Chengyun Li et Xuelin Tan. « Identification of the Core Pollen-Specific Regulation in the Rice OsSUT3 Promoter ». International Journal of Molecular Sciences 21, no 6 (11 mars 2020) : 1909. http://dx.doi.org/10.3390/ijms21061909.

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The regulatory mechanisms of pollen development have potential value for applications in agriculture, such as better understanding plant reproductive regularity. Pollen-specific promoters are of vital importance for the ectopic expression of functional genes associated with pollen development in plants. However, there is a limited number of successful applications using pollen-specific promoters in genetic engineering for crop breeding and hybrid generation. Our previous work led to the identification and isolation of the OsSUT3 promoter from rice. In this study, to analyze the effects of different putative regulatory motifs in the OsSUT3 promoter, a series of promoter deletions were fused to a GUS reporter gene and then stably introduced into rice and Arabidopsis. Histochemical GUS analysis of transgenic plants revealed that p385 (from −385 to −1) specifically mediated maximal GUS expression in pollen tissues. The S region (from −385 to −203) was the key region for controlling the pollen-specific expression of a downstream gene. The E1 (−967 to −606), E2 (−202 to −120), and E3 (−119 to −1) regions enhanced ectopic promoter activity to different degrees. Moreover, the p385 promoter could alter the expression pattern of the 35S promoter and improve its activity when they were fused together. In summary, the p385 promoter, a short and high-activity promoter, can function to drive pollen-specific expression of transgenes in monocotyledon and dicotyledon transformation experiments.
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Sun, Ye, Zi Lin, Anke Reinders et John M. Ward. « Functionally Important Amino Acids in Rice Sucrose Transporter OsSUT1 ». Biochemistry 51, no 15 (9 avril 2012) : 3284–91. http://dx.doi.org/10.1021/bi201934h.

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Sun, Ye, et John M. Ward. « Arg188 in rice sucrose transporter OsSUT1 is crucial for substrate transport ». BMC Biochemistry 13, no 1 (2012) : 26. http://dx.doi.org/10.1186/1471-2091-13-26.

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Siao, Wei, Jia-Yi Chen, Hui-Hsin Hsiao, Ping Chung et Shu-Jen Wang. « Characterization of OsSUT2 Expression and Regulation in Germinating Embryos of Rice Seeds ». Rice 4, no 2 (juin 2011) : 39–49. http://dx.doi.org/10.1007/s12284-011-9063-1.

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Chen, Jia-Yi, Shiang-Lin Liu, Wei Siao et Shu-Jen Wang. « Hormone and sugar effects on rice sucrose transporter OsSUT1 expression in germinating embryos ». Acta Physiologiae Plantarum 32, no 4 (12 janvier 2010) : 749–56. http://dx.doi.org/10.1007/s11738-009-0459-0.

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Ishimaru, Ken, Tatsurou Hirose, Naohiro Aoki, Sakiko Takahashi, Kiyomi Ono, Shinichi Yamamoto, Jiangzhong Wu et al. « Antisense Expression of a Rice Sucrose Transporter OsSUT1 in Rice (Oryza sativa L.) ». Plant and Cell Physiology 42, no 10 (15 octobre 2001) : 1181–85. http://dx.doi.org/10.1093/pcp/pce148.

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Xu, Xiameng, Zhengyin Xu, Ziyang Li, Muhammad Zakria, Lifang Zou et Gongyou Chen. « Increasing resistance to bacterial leaf streak in rice by editing the promoter of susceptibility gene OsSULRT3 ; 6 ». Plant Biotechnology Journal 19, no 6 (8 mai 2021) : 1101–3. http://dx.doi.org/10.1111/pbi.13602.

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13

Scofield, G. N., T. Hirose, N. Aoki et R. T. Furbank. « Involvement of the sucrose transporter, OsSUT1, in the long-distance pathway for assimilate transport in rice ». Journal of Experimental Botany 58, no 12 (13 juillet 2007) : 3155–69. http://dx.doi.org/10.1093/jxb/erm153.

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Siahpoosh, Mohammad R., Diego H. Sanchez, Armin Schlereth, Graham N. Scofield, Robert T. Furbank, Joost T. van Dongen et Joachim Kopka. « Modification of OsSUT1 gene expression modulates the salt response of rice Oryza sativa cv. Taipei 309 ». Plant Science 182 (janvier 2012) : 101–11. http://dx.doi.org/10.1016/j.plantsci.2011.01.001.

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Hirose, Tatsuro, Zujian Zhang, Akio Miyao, Hirohiko Hirochika, Ryu Ohsugi et Tomio Terao. « Disruption of a gene for rice sucrose transporter, OsSUT1, impairs pollen function but pollen maturation is unaffected ». Journal of Experimental Botany 61, no 13 (5 juillet 2010) : 3639–46. http://dx.doi.org/10.1093/jxb/erq175.

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Scofield, G. N., N. Aoki, T. Hirose, M. Takano, C. L. D. Jenkins et R. T. Furbank. « The role of the sucrose transporter, OsSUT1, in germination and early seedling growth and development of rice plants ». Journal of Experimental Botany 58, no 3 (6 décembre 2006) : 483–95. http://dx.doi.org/10.1093/jxb/erl217.

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17

Kato, Tsuneo, Ayano Taniguchi et Akira Horibata. « Effects of the Alleles at OsAGPS2 and OsSUT1 on the Grain Filling in Extra-Heavy Panicle Type of Rice ». Crop Science 50, no 6 (novembre 2010) : 2448–56. http://dx.doi.org/10.2135/cropsci2009.11.0690.

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Xiong, Yufei, Ye Ren, Wang Li, Fengsheng Wu, Wenjie Yang, Xiaolong Huang et Jialing Yao. « NF-YC12 is a key multi-functional regulator of accumulation of seed storage substances in rice ». Journal of Experimental Botany 70, no 15 (11 avril 2019) : 3765–80. http://dx.doi.org/10.1093/jxb/erz168.

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AbstractStarch and storage proteins, the primary storage substances of cereal endosperm, are a major source of food for humans. However, the transcriptional regulatory networks of the synthesis and accumulation of storage substances remain largely unknown. Here, we identified a rice endosperm-specific gene, NF-YC12, that encodes a putative nuclear factor-Y transcription factor subunit C. NF-YC12 is expressed in the aleurone layer and starchy endosperm during grain development. Knockout of NF-YC12 significantly decreased grain weight as well as altering starch and protein accumulation and starch granule formation. RNA-sequencing analysis revealed that in the nf-yc12 mutant genes related to starch biosynthesis and the metabolism of energy reserves were enriched in the down-regulated category. In addition, starch and protein contents in seeds differed between NF-YC12-overexpression lines and the wild-type. NF-YC12 was found to interact with NF-YB1. ChIP-qPCR and yeast one-hybrid assays showed that NF-YC12 regulated the rice sucrose transporter OsSUT1 in coordination with NF-YB1 in the aleurone layer. In addition, NF-YC12 was directly bound to the promoters of FLO6 (FLOURY ENDOSPERM6) and OsGS1;3 (glutamine synthetase1) in developing endosperm. This study demonstrates a transcriptional regulatory network involving NF-YC12, which coordinates multiple pathways to regulate endosperm development and the accumulation of storage substances in rice seeds.
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&NA;. « OSSUR INTRODUCES A NEW FLEX-FOOT? ? : THE CETERUS?? ? THE TORSION CONTROL CELL?? ? PROVIDES NATURAL, RESPONSIVE ROTATION ». JPO Journal of Prosthetics and Orthotics 14, no 2 (juin 2002) : A8. http://dx.doi.org/10.1097/00008526-200206000-00001.

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Matsukura, Chi-aki, Toshikazu Saitoh, Toshiro Hirose, Ryu Ohsugi, Pierdomenico Perata et Junji Yamaguchi. « Sugar Uptake and Transport in Rice Embryo. Expression of Companion Cell-Specific Sucrose Transporter (OsSUT1) Induced by Sugar and Light ». Plant Physiology 124, no 1 (1 septembre 2000) : 85–94. http://dx.doi.org/10.1104/pp.124.1.85.

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Chung, Ping, Hui-Hsin Hsiao, Huai-Ju Chen, Chia-Wei Chang et Shu-Jen Wang. « Influence of temperature on the expression of the rice sucrose transporter 4 gene, OsSUT4, in germinating embryos and maturing pollen ». Acta Physiologiae Plantarum 36, no 1 (16 octobre 2013) : 217–29. http://dx.doi.org/10.1007/s11738-013-1403-x.

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Ibraheem, O., C. E. J. Botha, G. Bradley, G. Dealtry et S. Roux. « Rice sucrose transporter1 (OsSUT1) up-regulation in xylem parenchyma is caused by aphid feeding on rice leaf blade vascular bundles ». Plant Biology 16, no 4 (11 novembre 2013) : 783–91. http://dx.doi.org/10.1111/plb.12117.

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Rakbangboon, Thanyaporn, Gary Guerra, Kazuhiko Sasaki, Pairat Tangpornprasert, Chanyaphan Virulsri, Sarawanee Phaipool, Jessica Hodges et al. « Affordable Energy Storage and Return Prosthesis : Functional Comparisons of the S-Pace and Ossur Vari-Flex Feet ». Archives of Physical Medicine and Rehabilitation 99, no 10 (octobre 2018) : e97. http://dx.doi.org/10.1016/j.apmr.2018.07.347.

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TAKAHASHI, Shinichiro, Ayano MEGURO-MAOKA et Midori YOSHIDA. « Analysis of Sugar Content and Expression of Sucrose Transporter Genes (OsSUTs) in Rice Tissues in Response to a Chilling Temperature ». Japan Agricultural Research Quarterly : JARQ 51, no 2 (2017) : 137–46. http://dx.doi.org/10.6090/jarq.51.137.

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Eom, Joon-Seob, Jung-Il Cho, Anke Reinders, Sang-Won Lee, Youngchul Yoo, Pham Quoc Tuan, Sang-Bong Choi et al. « Impaired Function of the Tonoplast-Localized Sucrose Transporter in Rice, OsSUT2, Limits the Transport of Vacuolar Reserve Sucrose and Affects Plant Growth ». Plant Physiology 157, no 1 (19 juillet 2011) : 109–19. http://dx.doi.org/10.1104/pp.111.176982.

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Ishibashi, Yushi, Kenta Okamura, Masayuki Miyazaki, Thuy Phan, Takashi Yuasa et Mari Iwaya-Inoue. « Expression of rice sucrose transporter gene OsSUT1 in sink and source organs shaded during grain filling may affect grain yield and quality ». Environmental and Experimental Botany 97 (janvier 2014) : 49–54. http://dx.doi.org/10.1016/j.envexpbot.2013.08.005.

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Miyazaki, Masayuki, Masato Araki, Kenta Okamura, Yushi Ishibashi, Takashi Yuasa et Mari Iwaya-Inoue. « Assimilate translocation and expression of sucrose transporter, OsSUT1, contribute to high-performance ripening under heat stress in the heat-tolerant rice cultivar Genkitsukushi ». Journal of Plant Physiology 170, no 18 (décembre 2013) : 1579–84. http://dx.doi.org/10.1016/j.jplph.2013.06.011.

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Kato, Tsuneo, et Akira Horibata. « Non-random distribution of the alleles for good grain filling at OsAGPS2 and OsSUT1 among a wide range of rice (Oryza sativa L.) cultivars ». Breeding Science 61, no 2 (2011) : 217–20. http://dx.doi.org/10.1270/jsbbs.61.217.

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Phan, T. T. T., Y. Ishibashi, M. Miyazaki, H. T. Tran, K. Okamura, S. Tanaka, J. Nakamura, T. Yuasa et M. Iwaya-Inoue. « High Temperature-Induced Repression of the Rice Sucrose Transporter (OsSUT1 ) and Starch Synthesis-Related Genes in Sink and Source Organs at Milky Ripening Stage Causes Chalky Grains ». Journal of Agronomy and Crop Science 199, no 3 (27 octobre 2012) : 178–88. http://dx.doi.org/10.1111/jac.12006.

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Talla, Hayder, Abdul Kareem Hassan et Jawad Oleiwi. « Study the Effect of Reinforcing Kevlar Fibers with Carbon Fibers and Glass Fibers on the Performance of the Athletic Prosthetic Foot ». Basrah journal for engineering science 22, no 2 (24 décembre 2022) : 41–48. http://dx.doi.org/10.33971/bjes.22.2.7.

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In this research, the mechanical properties were studied from the experimental, theoretical, and numerical aspects of the sports prosthetic foot for the purpose of providing a sporty prosthetic limb with high performance, easy to use and an appropriate financial cost to use by amputees who have lost their lower limbs (amputation below the knee) in practicing their sports activities and overcoming physical disability. The dimensions of the blades were calculated based on side profiles from European patent specifications. The chosen fibers have high strength, are light in weight, and can be purchased for a lower price than the materials that are used in the production of the sports prosthetic feet that are already on the market and are produced by specialized companies such as Ottobock and Ossur. Six laminates of the composite material consisting of matrix orthocryl lamination 80:20 pro reinforced with different fibers (Kevlar fibers, carbon fibers, glass fibers, and perlon fibers) were fabricated in the form of rectangles using the vacuum system and then cut to the required dimensions using a CNC machine. The density and volume fraction of the samples and the use of the rule of mixtures to calculate the mechanical properties of the laminates were calculated and entered into the ANSYS program. Then the boundary conditions were applied to the athlete's prosthetic foot and the total deformation, and the total strain energy was calculated to find out the best laminates in the athlete's foot industry. It was noticed that the laminates reinforced with carbon fibers were better than the laminates reinforced with glass fibers in terms of Young’s Modulus, as well as deformation. The best laminate obtained is (12 K + 4 C).
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Datta, D., I. Harris, B. Heller, J. Howitt et R. Martin. « Gait, cost and time implications for changing from PTB to ICEX®sockets ». Prosthetics and Orthotics International 28, no 2 (août 2004) : 115–20. http://dx.doi.org/10.1080/03093640408726696.

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The ICEX®system (Ossur, Iceland), allows a socket to be manufactured directly onto the stump and is thought to provide improved comfort due to better pressure distribution whilst being easier to fit and manufacture.The aims of this project were to a) compare gait performance by measuring several gait characteristics, b) compare production and fitting times, c) investigate financial implications and d) attempt to gauge the amputees’ subjective opinions of socket comfort.A randomised, controlled trial was conducted on 27 trans-tibial amputees with an existing patellar tendon bearing (PTB) socket on the Endolite™ system (Chas A. Blatchford, UK). Twenty one (21) subjects completed the study. Of these, 10 in the control group received new PTB sockets while 11 in the experimental group received ICEX®.Gait analysis wearing existing sockets was performed and kinetic data obtained from a force plate. This was repeated with the new sockets after a 6 week period of adjustment. Mann-Whitney tests were used in statistical evaluations with a significance level of 5%. Subjects were asked to score their prosthesis for comfort using the Socket Comfort Score (Hanspal et al., 2003) and the frequency of visits for socket adjustments over a three-month period post-delivery of the sockets was recorded.This study demonstrates no significant difference in any of the gait parameters measured. Though the time required to manufacture a PTB prosthesis was found to be considerably longer than the ICEX®, the overall cost for producing the ICEX®was significantly greater. Subjects showed only minor comfort preference for the ICEX®design and there was no significant difference in the mean number of visits for socket adjustments.In view of the considerable additional cost of providing ICEX®and the lack of evidence of improvement in any parameter tested, the routine provision of ICEX®prostheses to unselected trans-tibial amputees cannot be recommended.
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Loewen, Alex, Sophia Ulman, Ashley Erdman, Savannah Cooper, Kirsten Tulchin-Francis, Philip Wilson et Henry B. Ellis. « Effects of a Medial Unloader Brace on Gait Mechanics in Patients with Osteochondritis Dissecans ». Orthopaedic Journal of Sports Medicine 10, no 5_suppl2 (1 mai 2022) : 2325967121S0041. http://dx.doi.org/10.1177/2325967121s00411.

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Background: Non-operative treatment for osteochondritis dissecans (OCD) of the knee traditionally includes casting, bracing, and activity restrictions. An unloader brace is an option that may result in improved compliance due to the ability of the patient to continue daily activities while maintaining brace wear, and it may have biomechanical advantages for lesion healing. However, the biomechanical efficacy of a medial unloader brace has not been confirmed in a pediatric population. Hypothesis/Purpose: To determine whether the use of a medial unloader brace alters gait mechanics in patients with medial femoral condyle OCD. We hypothesized that while knee alignment in the coronal plane will remain unaltered, the valgus moment will decrease due to the applied tension of the adjusted unloader brace. Methods: Fifteen medial femoral condylar OCD patients (14 male, 12.7±2.6 years, 156.9±14.8 cm, and 52.0±16.2 kg) were tested utilizing a medial Ossur Unloader One knee brace. All patients performed over-ground walking at a self-selected speed under three brace conditions: no brace (NB), unadjusted (U), and adjusted (A; prescribed tension applied). Pelvis and lower extremity kinematic and kinetic data were computed in the sagittal, coronal, and transverse planes during single-limb stance phase (SLS). Wilcoxon signed-rank tests were performed to identify statistical differences between brace conditions ( α=0.05), and clinical significance was only determined when statistically significant joint angle and moment differences were greater than 3° and 0.1Nm/kg, respectively. Results: Between braced conditions, mean knee valgus was not statistically significant, however, peak internal knee valgus moment consistently decreased with each brace condition (NB-A: 0.06 Nm/kg, p=0.017). Similarly, hip abduction moment at the second peak of SLS decreased while wearing the brace (NB-U: p=0.061, NB-A: 0.07 Nm/kg, p=0.009). Conclusion: Peak knee valgus moment was decreased during walking with an adjusted medial unloader brace. These results highlight potential biomechanical advantages of a medial unloader brace for medial femoral condylar OCD. Future work will measure compliance of brace wear and compare additional brace designs. [Table: see text][Figure: see text]
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Zhang, Chunlong, Qiuping Li, Hong Yang, Tuo Wang, Juan Li, Jiancheng Wen, Shoulin Jin, Zhonglin Zhang, Lijuan Chen et Dandan Li. « A 31-bp indel localised in the 5' untranslated region of OsSUT3 affects the gene expression and rice (Oryza sativa L.) pollen development ». Czech Journal of Genetics and Plant Breeding, 1 novembre 2021. http://dx.doi.org/10.17221/66/2021-cjgpb.

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OsSUT genes have been demonstrated to be relevant for diverse biological processes in rice. In this study, we identified the close relationship between a 31-bp insertion in a 5' untranslated region (5' UTR) of the OsSUT3 gene and higher OsSUT3 expression in rice panicles by qRT-PCR and transgenic research. Statistically significant results (P < 0.01) were found for this 31-bp insertions/deletions (indels) in the rice pollen development and other panicle traits, such as the pollen number, pollen fertility, seeding rate, and grain length. An evolution analysis showed that the proportion of the 31-bp insertion significantly increases in rice domestication. Therefore, the 31-bp Indel could be considered as a convenient molecular marker to screen more pollen and better panicle traits in rice breeding.
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Li, Guohui, Kehui Cui, Qiuqian Hu, Wencheng Wang, Junfeng Pan, Guo Zhang, Yange Shi, Lixiao Nie, Jianliang Huang et Shaobing Peng. « Phloem Unloading in Developing Rice Caryopses and Its Contribution to Nonstructural Carbohydrate Translocation from Stems and Grain Yield Formation ». Plant and Cell Physiology, 10 août 2022. http://dx.doi.org/10.1093/pcp/pcac118.

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Abstract Phloem unloading plays an important role in photoassimilates partitioning and grain yield improvements in cereal crops. The phloem unloading strategy and its effects on photoassimilates translocation and yield formation remain unclear in rice. In this study, plasmodesmata were observed at the interface between the sieve elements (SEs) and companion cells (CCs), and between the SE-CC complex and surrounding parenchyma cells (PCs) in phloem of dorsal vascular bundle in developing caryopses. Carboxyfluorescein (CF) signal was detected in the phloem of caryopsis, which showed that CF was unloaded into caryopses. These results indicated that the SE-CC complex was symplasmically connected with adjacent PCs by plasmodesmata. Gene expression for sucrose transporter (SUT) and cell wall invertase (CWI) and protein of OsSUT1 and OsCIN1 were detected in developing caryopses, indicating that rice plants might actively unload sucrose into caryopses by apoplasmic pathway. Among three rice recombinant inbred lines, R201 exhibited lower plasmodesmal densities at the boundaries between cell types (SE-CC, SE-PC, CC-PC) in developing caryopses than R91 and R156. R201 also had lower expression level of SUT and CWI genes and protein levels of OsSUT1 and OsCIN1, and the CWI activity than R91 and R156. These data agreed with stem nonstructural carbohydrate (NSC) translocation and grain yields for the three lines. Nitrogen application rate had no significant effect on plasmodesmal densities at the interfaces between different cells types, and did not affect CF unloading in the phloem of developing caryopses. Low nitrogen treatment enhanced expression levels of OsSUTs and OsCINs in three lines. These results suggested that nitrogen application had no substantial effect on symplasmic unloading but affected apoplasmic unloading. Therefore, we concluded that poor symplasmic and apoplasmic unloading in developing caryopses might result in low stem NSC translocation and poor grain yield formation of R201.
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Li, Kangning, Shuo Tang, Shunan Zhang, Yanghao Tian, Hongye Qu, Mian Gu et Guohua Xu. « Rice circadian clock regulator Nhd1 controls sucrose transporter OsSUT1 expression and impacts carbon-nitrogen balance ». Journal of Experimental Botany, 14 décembre 2022. http://dx.doi.org/10.1093/jxb/erac494.

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Abstract Interdependent metabolic and transport processes of carbon (C) and nitrogen (N) regulate plant growth and development, while the regulatory pathways remain poorly defined. We previously reported that a rice circadian clock N-mediated heading date-1 (Nhd1) regulates growth-duration-dependent N use efficiency. Here, we report that knockout of Nhd1 in rice reduced photosynthesis rate and sucrose ratio of sheaths to blades but increased total C to N ratio and free amino acids. Leaf RNA-seq analysis indicated that mutation of Nhd1 dramatically altered expression of the genes linking to starch and sucrose metabolism, circadian rhythm, and amino acid metabolic pathways. We identified that Nhd1 can directly activate the transcriptional expression of sucrose transporter-1 (OsSUT1). Knockout of Nhd1 suppressed OsSUT1 expression and both nhd1 and ossut1 mutants showed similar shorter height, lower shoot biomass and sucrose concentration in comparison to wild type, while overexpression of OsSUT1 can restore the defective sucrose transport and partially ameliorate the reduced growth of nhd1 mutants. Nhd1-binding site of OsSUT1 promoter is conserved in all known rice genomes. The positively related variation of Nhd1 and OsSUT1 expression among randomly selected Indica and Japonica varieties suggests a common regulatory module of Nhd1-OsSUT1 mediated C and N balance in rice.
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Deng, Bin, Xiaoyan Gu, Sen Chen, Meng Zhang, Suwei Hao, Lixian Wei, Ying Cao et Shanglian Hu. « Genome-wide analysis and characterization of Dendrocalamus farinosus SUT gene family reveal DfSUT4 involvement in sucrose transportation in plants ». Frontiers in Plant Science 13 (20 janvier 2023). http://dx.doi.org/10.3389/fpls.2022.1118398.

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Sucrose is the main transported form of photosynthetic products. Sucrose transporter (SUT) participates in the translocation of sucrose from source to sink, which is important for the growth and development of plants. Dendrocalamus farinosus is an important economic crop in southwestern China because of its high growth rate, high fiber content, and dual usage for food and timber, but the mechanism of sucrose transportation in D. farinosus is unclear. In this study, a total of 12 SUT transporter genes were determined in D. farinosus by whole-genome identification. DfSUT2, DfSUT7, and DfSUT11 were homologs of rice OsSUT2, while DfSUT4 was a homolog of OsSUT4, and these four DfSUT genes were expressed in the leaf, internode, node, and bamboo shoots of D. farinosus. In addition, DfSUT family genes were involved in photosynthetic product distribution, ABA/MeJA responses, and drought resistance, especially DfSUT4. The function of DfSUT4 was then verified in Nicotiana tabacum. DfSUT4 was localized mainly in the leaf mesophyll and stem phloem of pDfSUT4::GUS transgenic plant. The overexpression of DfSUT4 gene in transgenic plant showed increases of photosynthetic rate, above-ground biomass, thousand grain weight, and cellulose content. Our findings altogether indicate that DfSUT4 can be a candidate gene that can be involved in phloem sucrose transportation from the source leaves to the sink organs, phytohormone responses, abiotic stress, and fiber formation in plants, which is very important in the genetic improvement of D. farinosus and other crops.
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Zhao, Zhigang, Chaolong Wang, Xiaowen Yu, Yunlu Tian, Wenxin Wang, Yunhui Zhang, Wenting Bai et al. « Auxin regulates source-sink carbohydrate partitioning and reproductive organ development in rice ». Proceedings of the National Academy of Sciences 119, no 36 (29 août 2022). http://dx.doi.org/10.1073/pnas.2121671119.

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Carbohydrate partitioning between the source and sink tissues plays an important role in regulating plant growth and development. However, the molecular mechanisms regulating this process remain poorly understood. In this study, we show that elevated auxin levels in the rice dao mutant cause increased accumulation of sucrose in the photosynthetic leaves but reduced sucrose content in the reproductive organs (particularly in the lodicules, anthers, and ovaries), leading to closed spikelets, indehiscent anthers, and parthenocarpic seeds. RNA sequencing analysis revealed that the expression of AUXIN RESPONSE FACTOR 18 ( OsARF18 ) and OsARF2 is significantly up- and down-regulated, respectively, in the lodicule of dao mutant. Overexpression of OsARF18 or knocking out of OsARF2 phenocopies the dao mutant. We demonstrate that OsARF2 regulates the expression of OsSUT1 through direct binding to the sugar-responsive elements (SuREs) in the OsSUT1 promoter and that OsARF18 represses the expression of OsARF2 and OsSUT1 via direct binding to the auxin-responsive element (AuxRE) or SuRE in their promoters, respectively. Furthermore, overexpression of OsSUT1 in the dao and Osarf2 mutant backgrounds could largely rescue the spikelets’ opening and seed-setting defects. Collectively, our results reveal an auxin signaling cascade regulating source-sink carbohydrate partitioning and reproductive organ development in rice.
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« Ossur Total Knee ». Biomedical Safety & ; Standards 41, no 11 (juin 2011) : 86. http://dx.doi.org/10.1097/01.bmsas.0000398929.53454.46.

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« Ossur Power Knee ». Biomedical Safety & ; Standards 52, no 21 (1 décembre 2022) : 165. http://dx.doi.org/10.1097/01.bmsas.0000902716.45942.41.

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« Ossur Lil Angel Vest ». Biomedical Safety & ; Standards 46, no 12 (juillet 2016) : 91–92. http://dx.doi.org/10.1097/01.bmsas.0000484717.55226.69.

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« Ossur Miami J Select Collar ». Biomedical Safety & ; Standards 51, no 15 (1 septembre 2021) : 118. http://dx.doi.org/10.1097/01.bmsas.0000768672.87539.b8.

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Wang, Xiaowen, Xiuli Liu, Zhi Hu, Shuhui Bao, Bing Feng, Lai Ma, Genmao Zhao, Dechun Zhang, Shubing Sun et Yibing Hu. « Essentiality for rice fertility and alternative splicing of OsSUT1 ». Plant Science, septembre 2021, 111065. http://dx.doi.org/10.1016/j.plantsci.2021.111065.

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Yang, Hong, Jin Chen, Chunlong Zhang, Khadija Tehseen Arshad, Qiuping Li, Tuo Wang, Jiancheng Wen et Dandan Li. « Construction of Rice <i>OsSUTs</i> ; Gene Knockout Lines Using ». Bioscience Methods, 2022. http://dx.doi.org/10.5376/bm.2022.13.0002.

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Zhou, Zhipeng, Yongze Yuan, Wei Zhou et Chufu Zhang. « Effects of exogenously supplied sucrose on OsSUTs and OsSPSs transcript abundances and rice root ammonium assimilation ». Acta Physiologiae Plantarum 38, no 12 (3 novembre 2016). http://dx.doi.org/10.1007/s11738-016-2285-5.

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Deng, Yao, Yongchao Yu, Yuxiang Hu, Li Ma, Yan Lin, Yue Wu, Zhen Wang et al. « Auxin-Mediated Regulation of Dorsal Vascular Cell Development May Be Responsible for Sucrose Phloem Unloading in Large Panicle Rice ». Frontiers in Plant Science 12 (25 février 2021). http://dx.doi.org/10.3389/fpls.2021.630997.

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Large panicle rice cultivars often fail to fulfill their high-yield potential due to the poor grain filling of inferior spikelets (IS), which appears as initially stagnant development and low final seed weight. Understanding the mechanism of the initial stagnancy is important to improve IS grain filling. In this study, superior spikelets (SS) were removed from two homozygous japonica rice varieties (W1844 and CJ03) with the same sink capacity in an attempt to force photosynthate transport to the IS. The results showed that SS removal increased the grain weight, sucrose content, starch accumulation, and endogenous IAA levels of IS during the initial grain-filling stage. SS removal also improved the patterns of vascular cells in the dorsal pericarp and the expression levels of genes involved in sucrose transport (OsSUTs and OsSWEETs) and IAA metabolism (OsYUCs and OsPINs). Exogenous IAA application advanced the initiation of grain filling by increasing the sucrose content and the gene expression levels of sucrose transporters. These results indicate that auxin may act like a signal substance and play a vital role in initial grain filling by regulating dorsal vascular cell development and sucrose phloem unloading into caryopsis.
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Kato, Tsuneo, Ryutaro Morita, Shinjiro Ootsuka, Yu Wakabayashi, Naohiro Aoki et Akira Horibata. « Evaluation of alleles at OsAGPS2 , OsAGPL2 , and OsSUT1 related to grain filling in rice in a common genetic background ». Crop Science, 8 décembre 2020. http://dx.doi.org/10.1002/csc2.20429.

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« Structural insights on Sucrose transport by Oryza sativa L. Sucrose/H+ Symporter1 (OsSUT1) through refined sequence - template alignment based structural modelling ». Indian Journal of Biochemistry and Biophysics, 2020. http://dx.doi.org/10.56042/ijbb.v57i3.36491.

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Jiang, Zhengrong, Qiuli Chen, Lin Chen, Hongyi Yang, Meichen Zhu, Yanfeng Ding, Weiwei Li, Zhenghui Liu, Yu Jiang et Ganghua Li. « Efficiency of Sucrose to Starch Metabolism Is Related to the Initiation of Inferior Grain Filling in Large Panicle Rice ». Frontiers in Plant Science 12 (13 septembre 2021). http://dx.doi.org/10.3389/fpls.2021.732867.

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The poor grain-filling initiation often causes the poor development of inferior spikelets (IS) which limits the yield potential of large panicle rice (Oryza sativa L.). However, it remains unclear why IS often has poor grain-filling initiation. In addressing this problem, this study conducted a field experiment involving two large panicle rice varieties, namely CJ03 and W1844, in way of removing the superior spikelets (SS) during flowering to force enough photosynthate transport to the IS. The results of this study showed that the grain-filling initiation of SS was much earlier than the IS in CJ03 and W1844, whereas the grain-filling initiation of IS in W1844 was evidently more promoted compared with the IS of CJ03 by removing spikelets. The poor sucrose-unloading ability, i.e., carbohydrates contents, the expression patterns of OsSUTs, and activity of CWI, were highly improved in IS of CJ03 and W1844 by removing spikelets. However, there was a significantly higher rise in the efficiency of sucrose to starch metabolism, i.e., the expression patterns of OsSUS4 and OsAGPL1 and activities of SuSase and AGPase, for IS of W1844 than that of CJ03. Removing spikelets also led to the changes in sugar signaling of T6P and SnRK1 level. These changes might be related to the regulation of sucrose to starch metabolism. The findings of this study suggested that poor sucrose-unloading ability delays the grain-filling initiation of IS. Nonetheless, the efficiency of sucrose to starch metabolism is also strongly linked with the grain-filling initiation of IS.
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Chen, Lin, Yao Deng, Honglei Zhu, Yuxiang Hu, Zhengrong Jiang, She Tang, Shaohua Wang et Yanfeng Ding. « The Initiation of Inferior Grain Filling is Affected by Sugar Translocation Efficiency in Large Panicle Rice ». Rice 12, no 1 (15 octobre 2019). http://dx.doi.org/10.1186/s12284-019-0333-7.

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Abstract Background Large panicle rice has a large sink capacity, but inferior spikelet filling is poor in this variety of rice due to asynchronous grain filling. The understanding of the factors that cause asynchronous grain filling will help to propose a model for how to regulate the rice inferior spikelets grain filling. Results In this study, two large panicle rice varieties, W1844 and CJ03, with the same sink capacity but with differences in asynchronous grain filling were used. The difference in the grain filling rate between superior and inferior spikelets in W1844 was much smaller than that in CJ03. We found that superior spikelet filling was initiated earlier in W1844 than in CJ03. The source-to-sink translocation rate of sucrose during the grain filling stage was more efficient in W1844 than in CJ03, and the gene expression levels of sucrose transporters (OsSUTs) were higher in W1844 functional leaves than in those of CJ03. In addition, carbon output, the transport ratio, and the contribution rate from the stem and sheath to the panicle were much higher at the early filling stage than at later filling stages in W1844. Conclusion Efficient sugar translocation can satisfy high sink strength, and also the strong sink activity can facilitate the sugar unloading in spikelets. All the above results indicate that an efficient sugar translocation rate at the early grain filling stage can improve sink strength and inferior grain filling initiation. Strategies to limit asynchronous grain filling in rice were also discussed based on our findings.
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Liu, Chen, Kai Chen, Xiuqin Zhao, Xiaoqian Wang, Congcong Shen, Yajun Zhu, Mingli Dai et al. « Identification of genes for salt tolerance and yield-related traits in rice plants grown hydroponically and under saline field conditions by genome-wide association study ». Rice 12, no 1 (décembre 2019). http://dx.doi.org/10.1186/s12284-019-0349-z.

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Abstract Background Soil salinity is one of the main environmental conditions that affects rice production. Identifying the genetic loci that affect rice salt tolerance (ST)-related traits at the seedling stage, especially under saline field conditions, is crucial for ST rice breeding by pyramiding ST genes that act at different developmental stages. Results Large phenotypic variations were observed in 708 rice accessions, and yield and its related traits were considerably limited when exposed to salt stress. In a genome-wide association study (GWAS), 2255 marker-trait association signals were detected for all measured traits, and the significant SNPs were distributed in 903 genes. Of these, 43 genes processed same functional annotation, and the gene ontology terms “biological processes” and “molecular function” with the known genes responsive to salt stress in rice. Further haplotype analysis detected 15 promising candidates significantly associated with the target traits, including five known genes and 10 novel genes. We identified seven accessions carrying favorable haplotypes of four genes significantly associated with grain yield that performed well under saline stress conditions. Conclusions Using high density SNPs within genes to conduct GWAS is an effective way to identify candidate genes for salt tolerance in rice. Five known genes (OsMYB6, OsGAMYB, OsHKT1;4, OsCTR3, and OsSUT1) and two newly identified genes (LOC_Os02g49700, LOC_Os03g28300) significantly associated with grain yield and its related traits under saline stress conditions were identified. These promising candidates provide valuable resources for validating potential ST-related genes and will facilitate rice breeding for salt tolerance through marker-assisted selection.
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