Bibliographies thématiques / Nucleic acid based drug

Littérature scientifique sur le sujet « Nucleic acid based drug »

Auteur : Grafiati
Publié le 11 mars 2023

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Articles de revues sur le sujet "Nucleic acid based drug"

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Tan, Xuyu, Fei Jia, Ping Wang et Ke Zhang. « Nucleic acid-based drug delivery strategies ». Journal of Controlled Release 323 (juillet 2020) : 240–52. http://dx.doi.org/10.1016/j.jconrel.2020.03.040.

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Kim, Haejoo, et Minseok Kwak. « Structures and Applications of Nucleic Acid-Based Micelles for Cancer Therapy ». International Journal of Molecular Sciences 24, no 2 (13 janvier 2023) : 1592. http://dx.doi.org/10.3390/ijms24021592.

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Nucleic acids have become important building blocks in nanotechnology over the last 30 years. DNA and RNA can sequentially build specific nanostructures, resulting in versatile drug delivery systems. Self-assembling amphiphilic nucleic acids, composed of hydrophilic and hydrophobic segments to form micelle structures, have the potential for cancer therapeutics due to their ability to encapsulate hydrophobic agents into their core and position functional groups on the surface. Moreover, DNA or RNA within bio-compatible micelles can function as drugs by themselves. This review introduces and discusses nucleic acid-based spherical micelles from diverse amphiphilic nucleic acids and their applications in cancer therapy.
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Kuriyama, Naoya, Yusuke Yoshioka, Shinsuke Kikuchi, Akihiko Okamura, Nobuyoshi Azuma et Takahiro Ochiya. « Challenges for the Development of Extracellular Vesicle-Based Nucleic Acid Medicines ». Cancers 13, no 23 (6 décembre 2021) : 6137. http://dx.doi.org/10.3390/cancers13236137.

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Nucleic acid drugs, such as siRNAs, antisense oligonucleotides, and miRNAs, exert their therapeutic effects by causing genetic changes in cells. However, there are various limitations in their delivery to target organs and cells, making their application to cancer treatment difficult. Extracellular vesicles (EVs) are lipid bilayer particles that are released from most cells, are stable in the blood, and have low immunogenicity. Methods using EVs to deliver nucleic acid drugs to target organs are rapidly being developed that take advantage of these properties. There are two main methods for loading nucleic acid drugs into EVs. One is to genetically engineer the parent cell and load the target gene into the EV, and the other is to isolate EVs and then load them with the nucleic acid drug. Target organ delivery methods include passive targeting using the enhanced permeation and retention effect of EVs and active targeting in which EVs are modified with antibodies, peptides, or aptamers to enhance their accumulation in tumors. In this review, we summarize the advantages of EVs as a drug delivery system for nucleic acid drugs, the methods of loading nucleic acid drugs into EVs, and the targeting of EVs to target organs.
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Wu, Yuanbing, Ania Rashidpour, María Pilar Almajano et Isidoro Metón. « Chitosan-Based Drug Delivery System : Applications in Fish Biotechnology ». Polymers 12, no 5 (21 mai 2020) : 1177. http://dx.doi.org/10.3390/polym12051177.

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Chitosan is increasingly used for safe nucleic acid delivery in gene therapy studies, due to well-known properties such as bioadhesion, low toxicity, biodegradability and biocompatibility. Furthermore, chitosan derivatization can be easily performed to improve the solubility and stability of chitosan–nucleic acid polyplexes, and enhance efficient target cell drug delivery, cell uptake, intracellular endosomal escape, unpacking and nuclear import of expression plasmids. As in other fields, chitosan is a promising drug delivery vector with great potential for the fish farming industry. This review highlights state-of-the-art assays using chitosan-based methodologies for delivering nucleic acids into cells, and focuses attention on recent advances in chitosan-mediated gene delivery for fish biotechnology applications. The efficiency of chitosan for gene therapy studies in fish biotechnology is discussed in fields such as fish vaccination against bacterial and viral infection, control of gonadal development and gene overexpression and silencing for overcoming metabolic limitations, such as dependence on protein-rich diets and the low glucose tolerance of farmed fish. Finally, challenges and perspectives on the future developments of chitosan-based gene delivery in fish are also discussed.
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de Vries, Jan Willem, Feng Zhang et Andreas Herrmann. « Drug delivery systems based on nucleic acid nanostructures ». Journal of Controlled Release 172, no 2 (décembre 2013) : 467–83. http://dx.doi.org/10.1016/j.jconrel.2013.05.022.

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Mulvey, Matthew C., Margaret Lemmon, Stephanie Rotter, Jonathan Lees, Leo Einck et Carol A. Nacy. « Optimization of a Nucleic Acid-Based Reporter System To Detect Mycobacterium tuberculosis Antibiotic Sensitivity ». Antimicrobial Agents and Chemotherapy 59, no 1 (3 novembre 2014) : 407–13. http://dx.doi.org/10.1128/aac.03135-14.

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ABSTRACTWe previously reported the development of a prototype antibiotic sensitivity assay to detect drug-resistantMycobacterium tuberculosisusing infection by mycobacteriophage to create a novel nucleic acid transcript, a surrogate marker of mycobacterial viability, detected by reverse transcriptase PCR (M. C. Mulvey et al., mBio3:e00312-11, 2012). This assay detects antibiotic resistance to all drugs, even drugs for which the resistance mechanism is unknown or complex: it is a phenotypic readout using nucleic acid detection. In this report, we describe development and characteristics of an optimized reporter system that directed expression of the RNA cyclase ribozyme, which generated circular RNA through an intramolecular splicing reaction and led to accumulation of a new nucleic acid sequence in phage-infected bacteria. These modifications simplified the assay, increased the limit of detection from 104to <102M. tuberculosiscells, and correctly identified the susceptibility profile ofM. tuberculosisstrains exposed for 16 h to either first-line or second-line antitubercular drugs. In addition to phenotypic drug resistance or susceptibility, the assay reported streptomycin MICs and clearly detected 10% drug-resistant cells in an otherwise drug-susceptible population.
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Ashrafuzzaman, Md. « Aptamers as Both Drugs and Drug-Carriers ». BioMed Research International 2014 (2014) : 1–21. http://dx.doi.org/10.1155/2014/697923.

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Aptamers are short nucleic acid oligos. They may serve as both drugs and drug-carriers. Their use as diagnostic tools is also evident. They can be generated using various experimental, theoretical, and computational techniques. The systematic evolution of ligands by exponential enrichment which uses iterative screening of nucleic acid libraries is a popular experimental technique. Theory inspired methodology entropy-based seed-and-grow strategy that designs aptamer templates to bind specifically to targets is another one. Aptamers are predicted to be highly useful in producing general drugs and theranostic drugs occasionally for certain diseases like cancer, Alzheimer’s disease, and so on. They bind to various targets like lipids, nucleic acids, proteins, small organic compounds, and even entire organisms. Aptamers may also serve as drug-carriers or nanoparticles helping drugs to get released in specific target regions. Due to better target specific physical binding properties aptamers cause less off-target toxicity effects. Therefore, search for aptamer based drugs, drug-carriers, and even diagnostic tools is expanding fast. The biophysical properties in relation to the target specific binding phenomena of aptamers, energetics behind the aptamer transport of drugs, and the consequent biological implications will be discussed. This review will open up avenues leading to novel drug discovery and drug delivery.
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Pozharov, Vitaly P., et Tamara Minko. « Nanotechnology-Based RNA Vaccines : Fundamentals, Advantages and Challenges ». Pharmaceutics 15, no 1 (5 janvier 2023) : 194. http://dx.doi.org/10.3390/pharmaceutics15010194.

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Over the past decades, many drugs based on the use of nanotechnology and nucleic acids have been developed. However, until recently, most of them remained at the stage of pre-clinical development and testing and did not find their way to the clinic. In our opinion, the main reason for this situation lies in the enormous complexity of the development and industrial production of such formulations leading to their high cost. The development of nanotechnology-based drugs requires the participation of scientists from many and completely different specialties including Pharmaceutical Sciences, Medicine, Engineering, Drug Delivery, Chemistry, Molecular Biology, Physiology and so on. Nevertheless, emergence of coronavirus and new vaccines based on nanotechnology has shown the high efficiency of this approach. Effective development of vaccines based on the use of nucleic acids and nanomedicine requires an understanding of a wide range of principles including mechanisms of immune responses, nucleic acid functions, nanotechnology and vaccinations. In this regard, the purpose of the current review is to recall the basic principles of the work of the immune system, vaccination, nanotechnology and drug delivery in terms of the development and production of vaccines based on both nanotechnology and the use of nucleic acids.
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Campuzano, Susana, María Pedrero et José M. Pingarrón. « Electrochemical Nucleic Acid-Based Biosensing of Drugs of Abuse and Pharmaceuticals ». Current Medicinal Chemistry 25, no 33 (24 octobre 2018) : 4102–18. http://dx.doi.org/10.2174/0929867324666171121103156.

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Background: Studies on the interactions of DNA with small molecular drugs are currently performed both to explore their mechanism of action and to develop new drugs. Electrochemical biosensors offer a very promising alternative to more complex conventional techniques for drug determination due to rapidness, low cost, simplicity, high sensitivity and compatibility with use in different settings. In this review, selected electrochemical nucleic acid-based biosensing methods described so far for the determination of pharmaceuticals and illicit drugs are briefly overviewed, discussing their basics and main features. A section pointing out general conclusions and future directions in this field is also provided. Results: The 42 selected contributions described electrochemical platforms to determine drugs of interest by monitoring their specific interactions with nucleic acids (DNA and aptamers), DNA damage and specific DNA-protein interactions. The highlighted approaches reported the use of electrodes unmodified or modified with nanomaterials and/or polymers in which DNA-drug interaction was followed by electrochemical detection of DNA puric bases, active drug or diffusion-free markers, and monitoring changes in the surface layer morphology/permeability and charge transfer resistance using different electrochemical techniques. Conclusion: Although electrochemical nucleic acid biosensing approaches constitute an interesting option for drugs determination in terms of cost, simplicity and miniaturized instrumentation, validating exhaustively their performance in real samples against conventional methodologies and implementing them into portable and automatic high throughput devices, together with exploring novel electrode modifications with nanomaterials and polymers and studying in more detail their multiplexing ability for analysis of a large number of analytes, is still needed.
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Du, Rong, Chen Wang, Ling Zhu et Yanlian Yang. « Extracellular Vesicles as Delivery Vehicles for Therapeutic Nucleic Acids in Cancer Gene Therapy : Progress and Challenges ». Pharmaceutics 14, no 10 (19 octobre 2022) : 2236. http://dx.doi.org/10.3390/pharmaceutics14102236.

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Extracellular vesicles (EVs) are nanoscale vesicles secreted by most types of cells as natural vehicles to transfer molecular information between cells. Due to their low toxicity and high biocompatibility, EVs have attracted increasing attention as drug delivery systems. Many studies have demonstrated that EV-loaded nucleic acids, including RNA-based nucleic acid drugs and CRISPR/Cas gene-editing systems, can alter gene expressions and functions of recipient cells for cancer gene therapy. Here in this review, we discuss the advantages and challenges of EV-based nucleic acid delivery systems in cancer therapy. We summarize the techniques and methods to increase EV yield, enhance nucleic acid loading efficiency, extend circulation time, and improve targeted delivery, as well as their applications in gene therapy and combination with other cancer therapies. Finally, we discuss the current status, challenges, and prospects of EVs as a therapeutic tool for the clinical application of nucleic acid drugs.
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Thèses sur le sujet "Nucleic acid based drug"

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Wong, Frances M. P. « Lipid-based vehicles for nucleic acid drugs ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape4/PQDD_0015/NQ56644.pdf.

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Satyal, Uttam. « Efficient Drug and Nucleic Acid Delivery Systems based on Synthetic Amphiphiles with Tuned Oil/Water Interfaces ». Diss., Temple University Libraries, 2018. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/531985.

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Pharmaceutical Sciences
Ph.D.
Today, drugs are an integral part of healthy human life, with new drug entities being introduced every year in clinic. The advancement of drug development brings complexity and variation, in terms of both physical and chemical properties. Some of these physicochemical characteristics are many times suboptimal, eventually requiring robust delivery systems that can precisely deliver the drugs to the desired tissues. Although many materials have been studied for the generation of drug delivery systems, there is always a need for biomaterials with better properties that can translate into superior delivery systems. In this context, new drug delivery systems that are interface-engineered at materials level for better stability and delivery efficiency in vitro and in vivo are introduced in this dissertation. In the first part of the dissertation, novel oil/water interface-engineered amphiphilic block copolymer micelles that were previously introduced by our lab were assessed for their stability in the presence of various esterase enzymes present in serum and on blood vessel walls, normally encountered by drug delivery systems on route to the targeted tissues. I also assessed the vulnerability of the polymeric micelles in presence of enzymes typically present either inside the tumor cells or secreted in the tumor microenvironment. I revealed the selective stability of empty- and docetaxel-loaded polymeric micelles to enzymatic degradation en route/in tumors and I have correlated this selective stability with polymer structure and interfacial engineering mentioned above. The unique delivery capabilities of interfacial-engineered polymeric micelles were tested in vivo using a mouse model of triple negative breast cancer. We proved that our novel engineered triblock copolymer-based drug delivery systems are superior to similar delivery systems made out of standard diblock copolymer micelles and also to the clinically used Taxotere® formulation towards cancer cell killing and tumor treatment, without displaying any significant toxicity in experimental animals. The second part of the dissertation focuses on the development and assessment of a pyridinium-based pseudo-gemini surfactant that combined the high nucleic acid packaging capacity of pyridinium lipids with the high transfection efficiency of gemini surfactants while displaying a reduced associated cytotoxic effect. I have analyzed the temperature treatment on compaction of nucleic acids into lipoplexes and I have established a high temperature annealing method for this purpose. This novel formulation technique allowed a substantial reduction of the amount of amphiphiles required to compact a specific amount of nucleic acids. This in turn also reduced the cytotoxic effect associated with the use of pyridinium amphiphiles. The effect of inclusion of colipids to lipoplex compaction, the robustness and the transfection efficiency of the lipid/nucleic acid lipoplex systems were assessed in detail, and correlations between formulation composition and biological activity were established. I was also able to show for the first time that pyridinium pseudo-gemini surfactants were able to compact different types of nucleic acids, including pDNA, mRNA and siRNA at lower charge ratios than standard, state-of-the art formulations used for this purposes. I also showed that irrespective to the nucleic acid compacted within the lipoplexes, the novel amphiphiles can efficiently deliver the cargo into the targeted cells even in the presence of very high concentration of serum, a premise for future use of these amphiphiles and formulations in vivo.
Temple University--Theses
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Pel, Joel. « A novel electrophoretic mechanism and separation parameter for selective nucleic acid concentration based on synchronous coefficient of drag alteration (SCODA) ». Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/13402.

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Molecular manipulation and separation techniques form the building blocks for much of fundamental science, yet many separation challenges still remain, in fields as diverse as forensics and metagenomics. This thesis presents SCODA (Synchronous Coefficient of Drag Alteration), a novel and general molecular separation and concentration technique aimed at addressing such challenges. SCODA takes advantage of physical molecular properties associated with the non‐linear response of long, charged polymers to electrophoretic fields, which define a novel parameter for DNA separation. The SCODA method is based on superposition of synchronous, time-varying electrophoretic fields, which can generate net drift of charged molecules even when the time-averaged molecule displacement generated by each field individually is zero. Such drift can only occur for molecules, such as DNA, whose motive response to electrophoretic fields is non-linear. This thesis presents the development of SCODA for extraction of DNA, and outlines the design of the instrumentation required to achieve the SCODA effect. We then demonstrate the selectivity, efficiency, and sensitivity of the technique. Contaminant rejection is also quantified for humic acids and proteins, with SCODA displaying excellent performance compared to existing technologies. Additionally, the ability of this technology to extract high molecular weight DNA is demonstrated, as is its inherent fragment length selection capability. Finally, we demonstrate two applications of this method to metagenomics projects where existing technologies performed poorly or failed altogether. The first is the extraction of high molecular weight DNA from soil, which is limited in length to fragments smaller than 50 kb with current direct extraction methods. SCODA was able to recover DNA an order of magnitude larger than this. The second application is DNA extraction from highly contaminated samples originating in the Athabasca tar sands, where existing technology had failed to recover any usable DNA. SCODA was able to recover sufficient DNA to enable the discovery of 200 putatively novel organisms.
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Folly, da Silva Constantino Laura. « An effective layered workflow of virtual screening for identification of active ligands of challenging protein targets ». Thesis, University of Iowa, 2017. https://ir.uiowa.edu/etd/5754.

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Docking is a computer simulation method used to predict the preferred orientation of two interacting chemical species that has been successfully applied to numerous macromolecules over the years. However, non-traditional targets have inherent difficulties associated with their screening. Large interfaces, lack of obvious binding sites, and transient pockets are some examples. Additionally, most natural ligands of challenging targets are inadequate models for identifying or designing new ligands. Therefore, it is not surprising that customary techniques of structure-based virtual screening are incompatible with these non-traditional targets. We hypothesized that an integrative virtual screening campaign comprised of docking followed by refinement of best receptor–ligand complexes would effectively identify small-molecule ligands of challenging receptors. We targeted the single-stranded DNA (ssDNA) binding groove of the human RAD52, and a cryptic allosteric pocket of the Helicobacter pylori Glutamate Racemase (GR). In this project, we first determined which docking method was more appropriate for each studied non-traditional target, and then examined how good our two-step docking workflow was in finding novel active ligand scaffolds. This research developed a powerful layered virtual screening workflow for the discovery of lead compounds against challenging protein targets. Furthermore, we successfully applied a statistical analysis method, which used receiver operating characteristic (ROC) curves, to validate the selected docking protocol that would be used in the screening campaigns. Using the validated workflow, we identified a natural compound that competes with ssDNA to bind to RAD52. The performed screening campaigns also provided new insights into the studied binding pockets, as well as structure-activity relationships (SAR) and binding determinants of the ligands. Our achievements reinforce the power of the ROC curve analysis approach in directing the search for the most appropriate docking protocol and helping to speed up drug discovery in pharmaceutical research.
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BELGIOVINE, GIULIANA. « Delivery of small interfering RNA into airway epithelial cells. Downregulation of the pro-inflammatory cytokine high mobility group BOX 1 ». Doctoral thesis, Università di Foggia, 2016. http://hdl.handle.net/11369/338921.

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polyplexes with cells. In conclusion, we have found optimal conditions for downregulation of HMGB1 by siRNA delivery mediated by polyaminoacidic polymers in airway epithelial cells in the absence of cytotoxicity. Functional and in-vivo studies are warranted. In the second part of this thesis, having demonstrated that the PHEA- and CSNAC- based nanoparticles are able to downregulate HMGB1 in airway epithelial cells, we sought to determine whether inflammatory conditions could regulate HMGB1 expression. H441 cells incubated with lipopolysaccharide (LPS) from Pseudomonas aeruginosa presented higher levels of HMGB1 as compared to untreated controls both as mRNA and protein as assessed by real time PCR and western blotting, respectively. This increase was observed both at 3 and 48 hours post treatment. LPS was also able to induce an increase in the metabolic state of cells (shown up by the MTT assay) at the same time points, but not in the proliferation (cell counts), while cell cycle analysis (by cytofluorimetry) showed a decrease of Go/G1 phase and an increase in G2/M at 6 and 24 h post-treatment. These data suggest that HMGB1 is involved in the activation of metabolic state of cells upon induction of an inflammatory condition, and it should further studied by siRNA downregulation.
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Akhras, Michael S. « Nucleic Acid Based Pathogen Diagnostics ». Doctoral thesis, KTH, Skolan för bioteknologi (BIO), 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4684.

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Pathogenic organisms are transmitted to the host organism through all possible connected pathways, and cause a myriad of diseases states. Commonly occurring curable infectious diseases still impose the greatest health impacts on a worldwide perspective. The Bill & Melinda Gates Foundation partnered with RAND Corporation to form the Global Health Diagnostics Forum, with the goal of establishing and interpreting mathematical models for what effects a newly introduced point-of-care pathogen diagnostic would have in developing countries. The results were astonishing, with potentially millions of lives to be saved on an annual basis. Golden standard for diagnostics of pathogenic bacteria has long been cultureable medias. Environmental biologists have estimated that less than 1% of all bacteria are cultureable. Genomic-based approaches offer the potential to identify all microbes from all the biological kingdoms. Nucleic acid based pathogen diagnostics has evolved significantly over the past decades. Novel technologies offer increased potential in sensitivity, specificity, decreased costs and parallel sample management. However, most methods are confined to core laboratory facilities. To construct an ultimate nucleic acid based diagnostic for use in areas of need, potential frontline techniques need to be identified and combined. The research focus of this doctoral thesis work has been to develop and apply nucleic acid based methods for pathogen diagnostics. Methods and assays were applied to the two distinct systems i) screening for antibiotic resistance mutations in the bacterial pathogen Neisseria gonorrhoeae, and ii) genotype determination of the cancer causative Human Papillomavirus (HPV). The first part of the study included development of rapid, direct and multiplex Pyrosequencing nucleic acid screenings. With improved methodology in the sample preparation process, we could detect an existence of multiple co-infecting HPV genotypes at greater sensitivities than previously described, when using the same type of methodology. The second part of the study focused on multiplex nucleic acid amplification strategies using Molecular Inversion Probes with end-step Pyrosequencing screening. The PathogenMip assay presents a complete detection schematic for virtually any known pathogenic organism. We also introduce the novel Connector Inversion Probe, a padlock probe capable of complete gap-fill reactions for multiplex nucleic acid amplifications.
Patogena organismer smittas till värd organismen genom alla möjliga kontaktnätverk och skapar en mångfald olika sjukdomstillstånd. Dock är det fortfarande vanligt förekommande behandlingsbara infektiösa sjukdomar som orsakar den största hälsoförlusten, sett från ett globalt perspektiv. Bill och Melinda Gates Stiftelsen samarbetade med RAND kooperation för att forma “The Global Health Diagnostics Forum”. Deras mål var att etablera och analysera matematiska modeller för vilka effekter en ny diagnostisk metod utrustat för fältarbete skulle ha i utvecklingsländer. Resultaten var häpnadsveckande, med potentiellt miljoner av liv som skulle kunna räddas på en årlig basis. Den etablerade standarden för diagnostik av patogena bakterier har länge varit kultiveringsmedia baserad. Miljö specialiserade biologer har estimerat att mindre än 1 % av alla bakterie arter går att kultivera. Dock erbjuder genetiska analyser potentialen att kunna identifiera alla mikrober från alla de biologiska rikena. Nukleinsyrebaserade diagnostiska metoder har märkbart förbättrats över de senaste årtionden. Nya tekniker erbjuder utökad sensitivitet, selektivitet, sänkta kostnader och parallella analyser av patient prover. Dock är de flesta metoderna begränsade till standardiserade laboratoriemiljöer. För att konstruera en väl fungerande diagnostisk fältutrustning för användning i problem områden, behöver världsledande tekniker identifieras och kombineras. Fokuseringsområdet för denna doktorsavhandling har varit att utveckla och utföra nukleinsyrebaserade metoder för patogen diagnostik. Metoder och experimentella utförande applicerades på två distinkta system i) sökning av antibiotika resistens relaterade mutationer i den patogena bakterien Neisseria gonorrhoeae och ii) genotypning av det cancer orsakande Humana Papillomaviruset (HPV). Den första delen av studien inriktade sig mot utveckling av snabba, direkta och multiplexa Pyrosekvenserings baserade nukleinsyreanalyser. Med förbättrad provprepareringsmetodologi kunde vi detektera multipla HPV infektioner med högre sensitivitet än vad tidigare beskrivits med liknande metodologi. Den andra delen av studien fokuserades på multiplexa nukleinsyre amplifikationer med “Molecular Inversion Probe” tekniken med sista steg Pyrosekvenserings analys. “PathogenMip assay” erbjuder ett komplett detektionsprotokoll för alla kända patogena organismer. Vi introducerar även den nya “Connector Inversion Probe”, en “Padlock Probe” kapabel att genomföra kompletta gap fyllningar för multiplex nukleinsyre amplifiering.
QC 20100624
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Elmén, Joacim. « Nucleic acid based therapeutic approaches / ». Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-047-8/.

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Allsop, Julie Kay. « Development of nucleic acid vaccines for mucosal delivery ». Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263104.

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Zhou, Chenguang. « NANOCARRIERS FOR THERAPEUTIC NUCLEIC ACID DELIVERY ». The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1336584204.

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O'Daniel, Peter Ivo. « Exploring structural diversity in nucleoside and nucleic acid drug design ». Diss., Available online, Georgia Institute of Technology, 2005, 2005. http://etd.gatech.edu/theses/available/etd-08252005-130946/.

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Thesis (Ph. D.)--Chemistry and Biochemistry, Georgia Institute of Technology, 2006.
Barefield, E. Kent, Committee Member ; Beckham, Haskell W., Committee Member ; Doyle, Donald F., Committee Member ; Weck, Marcus, Committee Member ; Seley, Katherine L., Committee Chair.
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Livres sur le sujet "Nucleic acid based drug"

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1946-, Propst C. L., et Perun Thomas J, dir. Nucleic acid targeted drug design. New York : M. Dekker, 1992.

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Methods for studying nucleic acid/drug interactions. Boca Raton : Taylor & Francis, 2012.

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Kim, Sung Wan. Pharmaceutical Perspectives of Nucleic Acid-Based Therapeutics. Sous la direction de Ram I. Mahato. Abingdon, UK : Taylor & Francis, 2002. http://dx.doi.org/10.4324/9780203300961.

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I, Mahato Ram, et Kim Sung Wan, dir. Pharmaceutical perspectives of nucleic acid-based therapeutics. London : Taylor & Francis, 2002.

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Pullman, Bernard, et Joshua Jortner, dir. Molecular Basis of Specificity in Nucleic Acid-Drug Interactions. Dordrecht : Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-011-3728-7.

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Luo, Yunbo. Functional Nucleic Acid Based Biosensors for Food Safety Detection. Singapore : Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-8219-1.

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Krul, Kenneth G. The nucleic acid-based therapeutics : World markets, developments and applications. Sous la direction de Heffner Steven et Kalorama Information LLC. New York, N.Y : Kalorama Information, 2005.

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Corporation, InteLab, dir. U.S. markets for nucleic acid probe-based diagnostic products, 1994-2001. Mission Viejo, CA : InteLab Corporation, 1996.

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Li, Tang. Development of liposome-based nucleic acid analyses for rapid detection of listeria monocytogenes. Ithaca, NY : Cornell University, 2003.

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Daly, Nathan Scott. Single-molecule studies of nucleic acid dynamics using carbon nanotube-based field-effect transistors. [New York, N.Y.?] : [publisher not identified], 2017.

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Chapitres de livres sur le sujet "Nucleic acid based drug"

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Zhang, A. Yin, et Susanna Wu-Pong. « Small Nucleic Acid-Based Drugs : Successes and Pitfalls ». Dans Biopharmaceutical Drug Design and Development, 193–221. Totowa, NJ : Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-532-9_10.

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Kardani, Sunil, et Devendra Vaishnav. « Potential Applications of Cationic Lipids in Nucleic Acid-Based Therapeutic Delivery System ». Dans Nanocarriers : Drug Delivery System, 329–47. Singapore : Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-33-4497-6_13.

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Mahmood, Mohammed Arif I., Umair J. M. Khan et Samir M. Iqbal. « Nucleic Acid-Based Encapsulations for Cancer Diagnostics and Drug Delivery ». Dans DNA and RNA Nanobiotechnologies in Medicine : Diagnosis and Treatment of Diseases, 163–87. Berlin, Heidelberg : Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-662-45775-7_7.

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Kim, Kyoung-Ran, Junghyun Kim et Dae-Ro Ahn. « Tissue-Specific Drug Delivery Platforms Based on DNA Nanoparticles ». Dans Handbook of Chemical Biology of Nucleic Acids, 1–28. Singapore : Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-16-1313-5_54-1.

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Faneca, Henrique, Ana Luísa Cardoso, Sara Trabulo, Sónia Duarte et Maria C. Pedroso de Lima. « Cationic Liposome-Based Systems for Nucleic Acid Delivery : From the Formulation Development to Therapeutic Applications ». Dans Drug Delivery Systems : Advanced Technologies Potentially Applicable in Personalised Treatment, 153–84. Dordrecht : Springer Netherlands, 2013. http://dx.doi.org/10.1007/978-94-007-6010-3_6.

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Higashi, Taishi, Keiichi Motoyama et Hidetoshi Arima. « Cyclodextrin-Based Drug Carriers for Low Molecular Weight Drugs, Proteins, and Nucleic Acids ». Dans Methods in Pharmacology and Toxicology, 27–45. New York, NY : Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3121-7_2.

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Pêgo, Ana Paula, Hugo Oliveira et Pedro Miguel Moreno. « Biomaterial-Based Vectors for Targeted Delivery of Nucleic Acids to the Nervous System ». Dans Drug Delivery Systems : Advanced Technologies Potentially Applicable in Personalised Treatment, 185–224. Dordrecht : Springer Netherlands, 2013. http://dx.doi.org/10.1007/978-94-007-6010-3_7.

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Zhang, Xiaolin, et Yunfeng Lin. « The Application and Problems of Tetrahedral Framework Nucleic Acids as a Drug Carrier in Biomedicine Fields ». Dans Advances in Nanomaterials-based Cell Biology Research, 137–66. Singapore : Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-2666-1_5.

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Lohmann, Sabine, Beatrix Bahle, Andrea Herold et Julian Schuster. « Formalin-Fixed Paraffin-Embedded Tissue (FFPET) Sections for Nucleic Acid-Based Analysis in Biomarker Discovery and Early Drug Development ». Dans Biomarkers in Disease : Methods, Discoveries and Applications, 187–219. Dordrecht : Springer Netherlands, 2015. http://dx.doi.org/10.1007/978-94-007-7696-8_24.

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Lohmann, Sabine, Beatrix Bahle, Andrea Herold et Julian Schuster. « Formalin-Fixed Paraffin-Embedded Tissue (FFPET) Sections for Nucleic Acid-Based Analysis in Biomarker Discovery and Early Drug Development ». Dans General Methods in Biomarker Research and their Applications, 1–26. Dordrecht : Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-007-7740-8_24-1.

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Actes de conférences sur le sujet "Nucleic acid based drug"

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Arshavsky-Graham, Sofia, Rita Vilneski, Federico Faratore, Moran Bercovici et Ester Segal. « 1,000-fold Sensitivity Enhancement of Porous Si-based Optical Biosensors for Nucleic Acid and Proteins Detection ». Dans Optical Molecular Probes, Imaging and Drug Delivery. Washington, D.C. : OSA, 2017. http://dx.doi.org/10.1364/omp.2017.omm4d.6.

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Vasilyeva, Svetlana, Nikolai Li-Zhulanov, Asya Levina, Valentina Zarytova et Vladimir Silnikov. « Drug delivery systems based on SiO2-nanoparticles bearing covalently bound triphosphates of nucleoside analogs ». Dans XVIth Symposium on Chemistry of Nucleic Acid Components. Prague : Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2014. http://dx.doi.org/10.1135/css201414394.

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Krasnoshtanova, Alla, et Elisaveta Borovkova. « OBTAINING NUCLEIC ACID PREPARATIONS AND THEIR HYDROLYSATES FROM BIOMASS OF METHANE-OXIDIZING BACTERIA ». Dans GEOLINKS Conference Proceedings. Saima Consult Ltd, 2021. http://dx.doi.org/10.32008/geolinks2021/b1/v3/14.

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"Due to the unfavourable environmental, social and economic situation, the need for the treatment of oncological diseases and diseases associated with impaired activity of the immune system is increasing. A lot of these drugs are made on the basis of nucleic acid components, the industrial production of which is practically non-existent in Russia. Therefore, a task of current interest is to develop the basis of the technology for obtaining components of nucleic acids, which can be widely used in medicine as immunomodulatory, wound-healing, antiviral, and diagnostic medicine, as well as for cancer treatment. Most of the described in literature methods of isolating nucleic acid components from plant, animal and microbial raw materials are based on the use of toxic and expensive organic solvents, that’s why it is impossible to apply these methods outside of laboratory conditions. The most promising source of raw materials for nucleic acids is the biomass of microorganisms (yeast and bacteria) from biomass, since the use of such source makes it possible to quickly obtain a large enough amount of biomass, and, consequently, a larger amount of nucleic acids. This allows obtaining DNA in addition to RNA. RNA and DNA substances can be used to obtain nucleosides and nitrogenous bases, which are also widely used in medicine. The purpose of these studies was to select the conditions for the extraction of RNA and DNA from the biomass of methane-oxidizing bacteria in one technological cycle, as well as to compare the efficiency of alkaline and acid hydrolysis of microbial RNA and DNA. The need for a two-stage extraction of nucleic acids from the biomass of methane-oxidizing bacteria in order to separately extract RNA and DNA was Substantiated. It was ascertained that at the first stage of extraction at a temperature of 90 ° C, pH 9.0 for 90 min, at least 85% of RNA is extracted. After the separation of the extract by centrifugation, the partially denuclearized biomass must be re-processed under the same conditions in order to extract DNA by at least 83%. The modes of concentration of RNA and DNA solutions by ultrafiltration were selected. It was found that in order to achieve effective deposition of nucleic acids at the isoelectric point, the concentration of the RNA solution must be carried out on the UPM-10 membrane at the concentration degree of 7, and the DNA solution on the UPM-100 membrane at the concentration degree 6. The dynamics of decomposition of nucleic-protein complexes in the medium of monoammonium phosphate was investigated. It was shown that the transition of NA into solution by at least 80% is achieved at a monoammonium phosphate concentration of 1.7 M, a temperature of 55 ° C for 90 min. The use of 5-fold washing of oligonucleotide substances with acidified water (pH 2.0) to remove excess mineral impurities was substantiated. А comparative assessment of acid and alkaline hydrolysis of RNA and DNA was carried out in order to obtain derivatives of nucleic acids."
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Meena, G. G., M. A. Stott, D. Ozcelik, T. A. Wall, R. Robison, A. R. Hawkins et H. Schmidt. « MMI waveguide based multispectral detection of nucleic acids for analysis of drug-resistant bacteria ». Dans 2016 IEEE Photonics Conference (IPC). IEEE, 2016. http://dx.doi.org/10.1109/ipcon.2016.7831137.

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Schramm, Vern L., Richard H. Furneaux, Peter C. Tyler et Gary B. Evans. « Enzymatic transition states and drug design ». Dans XVIth Symposium on Chemistry of Nucleic Acid Components. Prague : Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2014. http://dx.doi.org/10.1135/css201414032.

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Atkin, Stephen L., Sylvain Barrier, Stephen T. Beckett, Tom Brown, Grahame Mackenzie et Leigh Madden. « Towards the use of sporopollenin in drug delivery : Efficient encapsulation of oligonucleotides ». Dans XIIIth Symposium on Chemistry of Nucleic Acid Components. Prague : Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2005. http://dx.doi.org/10.1135/css200507307.

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Kiuru, Emilia, Mikko Ora, Leonid Beigelman, Lawrence Blatt et Harri Lönnberg. « On the feasibility of an esterase-dependent pro-drug strategy for 2-5A ». Dans XVth Symposium on Chemistry of Nucleic Acid Components. Prague : Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2011. http://dx.doi.org/10.1135/css201112184.

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Swarbrick, Joanna M., et Barry V. L. Potter. « Cyclic adenosine 5'-diphosphate ribose signalling : towards drug-like analogues to modulate CD38 and calcium release ». Dans XVIth Symposium on Chemistry of Nucleic Acid Components. Prague : Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2014. http://dx.doi.org/10.1135/css201414111.

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Satish, D. « Ionization Potentials of Nucleic Acid Intercalators ». Dans Functional Materials and Applied Physics. Materials Research Forum LLC, 2022. http://dx.doi.org/10.21741/9781644901878-12.

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Abstract. Nucleic acid based electronic devices have attracted particular interest over the past two decades due to its ability of long-range charge transport and self-assembly. The π-π interactions of the stacked bases are believed to be responsible for the long-range charge transport. The insertion of intercalators could alter electronic structure of the host nucleic acids which may influence the charge transport through the nucleic acid. The influence of intercalators on charge transport through the host nucleic acids largely depends on ionization potentials of the intercalators. Therefore, in this work we intend to determine vertical and adiabatic ionization potentials of the nucleic acid intercalators by using density functional theory calculations using Gaussian 16 package. We also explore the role of solvent and discuss the significance of ionization potential values in comparison with the ionization potential values of nucleic acid bases. Ionization Potential values of these intercalators range from 7.67 eV to 11.12 eV and 4.5 eV to 6.46 eV in vacuum and aqueous medium, respectively. Daunomycin is found to have lowest ionization potential value in vacuum as well as in aqueous medium. On the other hand, Proflavine (Anthraquinone) has highest ionization potential value in vacuum (aqueous medium). Non-planar intercalators exhibit distinct vertical and adiabatic ionization potential values and decrease drastically upon solvation.
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Labuda, Jan. « Concepts and terms at electrochemical nucleic acid-based biosensors ». Dans 2009 2nd International Symposium on Applied Sciences in Biomedical and Communication Technologies (ISABEL). IEEE, 2009. http://dx.doi.org/10.1109/isabel.2009.5373647.

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Rapports d'organisations sur le sujet "Nucleic acid based drug"

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Lebron, Carmen, Erik Petrovskis, Frank Loffler et Keith Henn. Use of Nucleic Acid-Based Tools for Monitoring Biostimulation and Bioaugmentation. Fort Belvoir, VA : Defense Technical Information Center, janvier 2011. http://dx.doi.org/10.21236/ada546746.

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Vangelas, K., E. ELIZABETH EDWARDS, F. FRANK LOFFLER et B. Brian02 Looney. ADVANCEMENT OF NUCLEIC ACID-BASED TOOLS FOR MONITORING IN SITU REDUCTIVE DECHLORINATION. US : SRS, novembre 2006. http://dx.doi.org/10.2172/898370.

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Kingsley, Mark T. Nucleic Acid-Based Detection and Identification of Bacterial and Fungal Plant Pathogens - Final Report. Office of Scientific and Technical Information (OSTI), mars 2001. http://dx.doi.org/10.2172/781863.

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Kingsley, Mark T. Nucleic Acid-Based Detection and Identification of Bacterial and Fungal Plant Pathogens - Final Report. Office of Scientific and Technical Information (OSTI), mars 2001. http://dx.doi.org/10.2172/965696.

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Cuevas, Christian, Jennifer De Lurio, Andrew Furman, Randy Hulshizer, Marcus Lynch et Prital Patel. PCORI COVID-19 Scan : BinaxNOW COVID-19 Ag Card, Saliva-based Nucleic Acid Assays (September 3-16, 2020). Patient-Centered Outcomes Research Institute (PCORI), septembre 2020. http://dx.doi.org/10.25302/bcs9.2020.9.

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Semaan, Dima, et Linda Scobie. Feasibility study for in vitro analysis of infectious foodborne HEV. Food Standards Agency, septembre 2022. http://dx.doi.org/10.46756/sci.fsa.wfa626.

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Hepatitis E virus (HEV) is a member of the Hepeviridae family capable of infecting humans producing a range of symptoms from mild disease to kidney failure. Epidemiological evidence suggests that hepatitis E genotype III and IV cases may be associated with the consumption of undercooked pork meat, offal and processed products such as sausages [1]. A study carried out by the Animal Health and Veterinary Laboratories Agency (AHVLA), found hepatitis E virus contamination in the UK pork production chain and that 10% of a small sample of retail pork sausages were contaminated with the virus [2]. Furthermore, studies have confirmed the presence of HEV in the food chain and the foodborne transmission of Hepatitis E virus to humans [reviewed in 5]. Likewise, Scottish shellfish at retail [6] have also been found positive for HEV viral nucleic acid and some preliminary studies indicate that the virus is also detectable in soft fruits (L Scobie; unpublished data). There are current misunderstandings in what this data represents, and these studies have raised further questions concerning the infectivity of the virus, the processing of these foods by industry and the cooking and/or preparation by caterers and consumers. There are significant gaps in the knowledge around viral infectivity, in particular the nature of the preparation of food matrices to isolate the virus, and also with respect to a consistent and suitable assay for confirming infectivity [1,3]. Currently, there is no suitable test for infectivity, and, in addition, we have no knowledge if specific food items would be detrimental to cells when assessing the presence of infectious virus in vitro. The FSA finalised a comprehensive critical review on the approaches to assess the infectivity of the HEV virus which is published [3] recommending that a cell culture based method should be developed for use with food. In order to proceed with the development of an infectivity culture method, there is a requirement to assess if food matrices are detrimental to cell culture cell survival. Other issues that may have affected the ability to develop a consistent method are the length of time the virally contaminated sample is exposed to the cells and the concentration of the virus present. In most cases, the sample is only exposed to the cells for around 1 hour and it has been shown that if the concentration is less that 1x103 copies then infection is not established [3,5,10,11].
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Porat, Ron, Gregory T. McCollum, Amnon Lers et Charles L. Guy. Identification and characterization of genes involved in the acquisition of chilling tolerance in citrus fruit. United States Department of Agriculture, décembre 2007. http://dx.doi.org/10.32747/2007.7587727.bard.

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Citrus, like many other tropical and subtropical fruit are sensitive to chilling temperatures. However, application of a pre-storage temperature conditioning (CD) treatment at 16°C for 7 d or of a hot water brushing (HWB) treatment at 60°C for 20 sec remarkably enhances chilling tolerance and reduces the development of chilling injuries (CI) upon storage at 5°C. In the current research, we proposed to identify and characterize grapefruit genes that are induced by CD, and may contribute to the acquisition of fruit chilling tolerance, by two different molecular approaches: cDNA array analysis and PCR cDNA subtraction. In addition, following the recent development and commercialization of the new Affymetrix Citrus Genome Array, we further performed genome-wide transcript profiling analysis following exposure to CD and chilling treatments. To conduct the cDNA array analysis, we constructed cDNA libraries from the peel tissue of CD- and HWB-treated grapefruit, and performed an EST sequencing project including sequencing of 3,456 cDNAs from each library. Based on the obtained sequence information, we chose 70 stress-responsive and chilling-related genes and spotted them on nylon membranes. Following hybridization the constructed cDNA arrays with RNA probes from control and CD-treated fruit and detailed confirmations by RT-PCR analysis, we found that six genes: lipid-transfer protein, metallothionein-like protein, catalase, GTP-binding protein, Lea5, and stress-responsive zinc finger protein, showed higher transcript levels in flavedo of conditioned than in non-conditioned fruit stored at 5 ᵒC. The transcript levels of another four genes: galactinol synthase, ACC oxidase, temperature-induced lipocalin, and chilling-inducible oxygenase, increased only in control untreated fruit but not in chilling-tolerant CD-treated fruit. By PCR cDNA subtraction analysis we identified 17 new chilling-responsive and HWB- and CD-induced genes. Overall, characterization of the expression patterns of these genes as well as of 11 more stress-related genes by RNA gel blot hybridizations revealed that the HWB treatment activated mainly the expression of stress-related genes(HSP19-I, HSP19-II, dehydrin, universal stress protein, EIN2, 1,3;4-β-D-glucanase, and SOD), whereas the CD treatment activated mainly the expression of lipid modification enzymes, including fatty acid disaturase2 (FAD2) and lipid transfer protein (LTP). Genome wide transcriptional profiling analysis using the newly developed Affymetrix Citrus GeneChip® microarray (including 30,171 citrus probe sets) revealed the identification of three different chilling-related regulons: 1,345 probe sets were significantly affected by chilling in both control and CD-treated fruits (chilling-response regulon), 509 probe sets were unique to the CD-treated fruits (chilling tolerance regulon), and 417 probe sets were unique to the chilling-sensitive control fruits (chilling stress regulon). Overall, exposure to chilling led to expression governed arrest of general cellular metabolic activity, including concretive down-regulation of cell wall, pathogen defense, photosynthesis, respiration, and protein, nucleic acid and secondary metabolism. On the other hand, chilling enhanced various adaptation processes, such as changes in the expression levels of transcripts related to membranes, lipid, sterol and carbohydrate metabolism, stress stimuli, hormone biosynthesis, and modifications in DNA binding and transcription factors.
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