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Littérature scientifique sur le sujet « Nanodomini »

Auteur : Grafiati
Publié le 9 mars 2023
Mis à jour le 10 mars 2023

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Articles de revues sur le sujet "Nanodomini"

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Okamoto, Yukihiro, Kaito Hamaguchi, Mayo Watanabe, Nozomi Watanabe et Hiroshi Umakoshi. « Characterization of Phase Separated Planar Lipid Bilayer Membrane by Fluorescence Ratio Imaging and Scanning Probe Microscope ». Membranes 12, no 8 (9 août 2022) : 770. http://dx.doi.org/10.3390/membranes12080770.

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The lipid membrane forms nanodomains (rafts) and shows heterogeneous properties. These nanodomains relate to significant roles in various cell functions, and thus the analysis of the nanodomains in phase-separated lipid membranes is important to clarify the function and role of the nanodomains. However, the lipid membrane possesses small-sized nanodomains and shows a small height difference between the nanodomains and their surroundings at certain lipid compositions. In addition, nanodomain analysis sometimes requires highly sensitive and expensive apparatus, such as a two-photon microscope. These have prevented the analysis by the conventional fluorescence microscope and by the topography of the scanning probe microscope (SPM), even though these are promising methods in macroscale and microscale analysis, respectively. Therefore, this study aimed to overcome these problems in nanodomain analysis. We successfully demonstrated that solvatochromic dye, LipiORDER, could analyze the phase state of the lipid membrane at the macroscale with low magnification lenses. Furthermore, we could prove that the phase mode of SPM was effective in the visualization of specific nanodomains by properties difference as well as topographic images of SPM. Hence, this combination method successfully gave much information on the phase state at the micro/macro scale, and thus this would be applied to the analysis of heterogeneous lipid membranes.
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Drab, Mitja, David Stopar, Veronika Kralj-Iglič et Aleš Iglič. « Inception Mechanisms of Tunneling Nanotubes ». Cells 8, no 6 (21 juin 2019) : 626. http://dx.doi.org/10.3390/cells8060626.

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Tunneling nanotubes (TNTs) are thin membranous tubes that interconnect cells, representing a novel route of cell-to-cell communication and spreading of pathogens. TNTs form between many cell types, yet their inception mechanisms remain elusive. We review in this study general concepts related to the formation and stability of membranous tubular structures with a focus on a deviatoric elasticity model of membrane nanodomains. We review experimental evidence that tubular structures initiate from local membrane bending facilitated by laterally distributed proteins or anisotropic membrane nanodomains. We further discuss the numerical results of several theoretical and simulation models of nanodomain segregation suggesting the mechanisms of TNT inception and stability. We discuss the coupling of nanodomain segregation with the action of protruding cytoskeletal forces, which are mostly provided in eukaryotic cells by the polymerization of f-actin, and review recent inception mechanisms of TNTs in relation to motor proteins.
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Liang, Pengbo, Thomas F. Stratil, Claudia Popp, Macarena Marín, Jessica Folgmann, Kirankumar S. Mysore, Jiangqi Wen et Thomas Ott. « Symbiotic root infections in Medicago truncatula require remorin-mediated receptor stabilization in membrane nanodomains ». Proceedings of the National Academy of Sciences 115, no 20 (30 avril 2018) : 5289–94. http://dx.doi.org/10.1073/pnas.1721868115.

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Plant cell infection is tightly controlled by cell surface receptor-like kinases (RLKs). Like other RLKs, the Medicago truncatula entry receptor LYK3 laterally segregates into membrane nanodomains in a stimulus-dependent manner. Although nanodomain localization arises as a generic feature of plant membrane proteins, the molecular mechanisms underlying such dynamic transitions and their functional relevance have remained poorly understood. Here we demonstrate that actin and the flotillin protein FLOT4 form the primary and indispensable core of a specific nanodomain. Infection-dependent induction of the remorin protein and secondary molecular scaffold SYMREM1 results in subsequent recruitment of ligand-activated LYK3 and its stabilization within these membrane subcompartments. Reciprocally, the majority of this LYK3 receptor pool is destabilized at the plasma membrane and undergoes rapid endocytosis in symrem1 mutants on rhizobial inoculation, resulting in premature abortion of host cell infections. These data reveal that receptor recruitment into nanodomains is indispensable for their function during host cell infection.
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Kim, Kyou-Hyun, et Jian-Min Zuo. « Convergent-beam electron-diffraction-pattern symmetry of nanodomains in complex lead-based perovskite crystals ». Acta Crystallographica Section A Foundations and Advances 70, no 6 (20 septembre 2014) : 583–90. http://dx.doi.org/10.1107/s2053273314013643.

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Convergent-beam electron diffraction (CBED) recorded using nanometre-sized probes, in principle, can detect the highest symmetry in a crystal. However, symmetry reduction may occur by overlapping crystal domains along the beam direction. Thus, delineating the relationship between the recorded and the crystal symmetry is important for studying crystals with complex nanodomains. This paper reports a study of the averaged local symmetry of 71°/109° rhombohedral (R), 90° tetragonal (T) and 180° monoclinic (M) nanodomain structures. The averaged symmetry of nanodomain structures is investigated by CBED simulations using the multislice method. The simulation results show that the 71°-R, 109°-R and 90°-T nanodomain structures partially mimic the monoclinic symmetries ofCmandPmthat have been proposed by the adaptive phase model. This study is also compared to the reported experimental CBED patterns recorded from PMN-31%PT.
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Lipke, Peter N., Caleen Ramsook, Melissa C. Garcia-Sherman, Desmond N. Jackson, Cho X. J. Chan, Michael Bois et Stephen A. Klotz. « Between Amyloids and Aggregation Lies a Connection with Strength and Adhesion ». New Journal of Science 2014 (2 février 2014) : 1–12. http://dx.doi.org/10.1155/2014/815102.

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We tell of a journey that led to discovery of amyloids formed by yeast cell adhesins and their importance in biofilms and host immunity. We begin with the identification of the adhesin functional amyloid-forming sequences that mediate fiber formation in vitro. Atomic force microscopy and confocal microscopy show 2-dimensional amyloid “nanodomains” on the surface of cells that are activated for adhesion. These nanodomains are arrays of adhesin molecules that bind multivalent ligands with high avidity. Nanodomains form when adhesin molecules are stretched in the AFM or under laminar flow. Treatment with anti-amyloid perturbants or mutation of the amyloid sequence prevents adhesion nanodomain formation and activation. We are now discovering biological consequences. Adhesin nanodomains promote formation and maintenance of biofilms, which are microbial communities. Also, in abscesses within candidiasis patients, we find adhesin amyloids on the surface of the fungi. In both human infection and a Caenorhabditis elegans infection model, the presence of fungal surface amyloids elicits anti-inflammatory responses. Thus, this is a story of how fungal adhesins respond to extension forces through formation of cell surface amyloid nanodomains, with key consequences for biofilm formation and host responses.
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Fukata, Yuko, Ariane Dimitrov, Gaelle Boncompain, Ole Vielemeyer, Franck Perez et Masaki Fukata. « Local palmitoylation cycles define activity-regulated postsynaptic subdomains ». Journal of Cell Biology 202, no 1 (8 juillet 2013) : 145–61. http://dx.doi.org/10.1083/jcb.201302071.

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Distinct PSD-95 clusters are primary landmarks of postsynaptic densities (PSDs), which are specialized membrane regions for synapses. However, the mechanism that defines the locations of PSD-95 clusters and whether or how they are reorganized inside individual dendritic spines remains controversial. Because palmitoylation regulates PSD-95 membrane targeting, we combined a conformation-specific recombinant antibody against palmitoylated PSD-95 with live-cell super-resolution imaging and discovered subsynaptic nanodomains composed of palmitoylated PSD-95 that serve as elementary units of the PSD. PSD-95 in nanodomains underwent continuous de/repalmitoylation cycles driven by local palmitoylating activity, ensuring the maintenance of compartmentalized PSD-95 clusters within individual spines. Plasma membrane targeting of DHHC2 palmitoyltransferase rapidly recruited PSD-95 to the plasma membrane and proved essential for postsynaptic nanodomain formation. Furthermore, changes in synaptic activity rapidly reorganized PSD-95 nano-architecture through plasma membrane–inserted DHHC2. Thus, the first genetically encoded antibody sensitive to palmitoylation reveals an instructive role of local palmitoylation machinery in creating activity-responsive PSD-95 nanodomains, contributing to the PSD (re)organization.
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Dai, Xunhu, Z. Xu, Jie-Fang Li et Dwight Viehland. « Effects of lanthanum modification on rhombohedral Pb(Zr1−xTix)O3 ceramics : Part II. Relaxor behavior versus enhanced antiferroelectric stability ». Journal of Materials Research 11, no 3 (mars 1996) : 626–38. http://dx.doi.org/10.1557/jmr.1996.0076.

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Lanthanum-modified lead zirconate titanate ceramics Pb1−3/2xLax(Zr1−yTiy)O3 [PLZT 100x/100(1 − y)/100y] with Zr/Ti ratios close to the antiferroelectric-ferroelectric (AFE-FE) phase boundary were investigated by dielectric spectroscopy, Sawyer–Tower polarization techniques, and electron microscopy. An incommensurate antiferroelectric (AFEIn) phase was found to be stabilized from the rhombohedral FE state in the compositional series 100x/90/10 for x ≥ 0.02. The La content required to induce the AFEIn state increased as the Ti content was increased. For 100x/85/15, a state with relaxor-like dielectric behavior and nanodomains was observed to develop with increasing La content; however, the double-loop-like P-E curves were suggestive of antiferroelectric behavior. Investigations for the composition 6/85/15 revealed the formation of nanodomains from the AFEIn modulation, where the size of the nanodomains equaled the value of the AFEIn modulation wavelength. For this composition, P-E studies revealed double hysteresis characteristics, whereas dielectric investigations revealed relaxor-like behavior. It is suggested that the order within the nanodomain state may be antipolar over a range of compositions in high La content rhombohedral PLZT ceramics.
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Stelate, Ayoub, Eva Tihlaříková, Kateřina Schwarzerová, Vilém Neděla et Jan Petrášek. « Correlative Light-Environmental Scanning Electron Microscopy of Plasma Membrane Efflux Carriers of Plant Hormone Auxin ». Biomolecules 11, no 10 (26 septembre 2021) : 1407. http://dx.doi.org/10.3390/biom11101407.

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Fluorescence light microscopy provided convincing evidence for the domain organization of plant plasma membrane (PM) proteins. Both peripheral and integral PM proteins show an inhomogeneous distribution within the PM. However, the size of PM nanodomains and protein clusters is too small to accurately determine their dimensions and nano-organization using routine confocal fluorescence microscopy and super-resolution methods. To overcome this limitation, we have developed a novel correlative light electron microscopy method (CLEM) using total internal reflection fluorescence microscopy (TIRFM) and advanced environmental scanning electron microscopy (A-ESEM). Using this technique, we determined the number of auxin efflux carriers from the PINFORMED (PIN) family (NtPIN3b-GFP) within PM nanodomains of tobacco cell PM ghosts. Protoplasts were attached to coverslips and immunostained with anti-GFP primary antibody and secondary antibody conjugated to fluorochrome and gold nanoparticles. After imaging the nanodomains within the PM with TIRFM, the samples were imaged with A-ESEM without further processing, and quantification of the average number of molecules within the nanodomain was performed. Without requiring any post-fixation and coating procedures, this method allows to study details of the organization of auxin carriers and other plant PM proteins.
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Huang, Dingquan, Yanbiao Sun, Zhiming Ma, Meiyu Ke, Yong Cui, Zichen Chen, Chaofan Chen et al. « Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization ». Proceedings of the National Academy of Sciences 116, no 42 (1 octobre 2019) : 21274–84. http://dx.doi.org/10.1073/pnas.1911892116.

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Plasmodesmata (PD) are plant-specific membrane-lined channels that create cytoplasmic and membrane continuities between adjacent cells, thereby facilitating cell–cell communication and virus movement. Plant cells have evolved diverse mechanisms to regulate PD plasticity in response to numerous environmental stimuli. In particular, during defense against plant pathogens, the defense hormone, salicylic acid (SA), plays a crucial role in the regulation of PD permeability in a callose-dependent manner. Here, we uncover a mechanism by which plants restrict the spreading of virus and PD cargoes using SA signaling by increasing lipid order and closure of PD. We showed that exogenous SA application triggered the compartmentalization of lipid raft nanodomains through a modulation of the lipid raft-regulatory protein, Remorin (REM). Genetic studies, superresolution imaging, and transmission electron microscopy observation together demonstrated that Arabidopsis REM1.2 and REM1.3 are crucial for plasma membrane nanodomain assembly to control PD aperture and functionality. In addition, we also found that a 14-3-3 epsilon protein modulates REM clustering and membrane nanodomain compartmentalization through its direct interaction with REM proteins. This study unveils a molecular mechanism by which the key plant defense hormone, SA, triggers membrane lipid nanodomain reorganization, thereby regulating PD closure to impede virus spreading.
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Traeger, Jeremiah, Dehong Hu, Mengran Yang, Gary Stacey et Galya Orr. « Super-Resolution Imaging of Plant Receptor-Like Kinases Uncovers Their Colocalization and Coordination with Nanometer Resolution ». Membranes 13, no 2 (21 janvier 2023) : 142. http://dx.doi.org/10.3390/membranes13020142.

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Plant cell signaling often relies on the cellular organization of receptor-like kinases (RLKs) within membrane nanodomains to enhance signaling specificity and efficiency. Thus, nanometer-scale quantitative analysis of spatial organizations of RLKs could provide new understanding of mechanisms underlying plant responses to environmental stress. Here, we used stochastic optical reconstruction fluorescence microscopy (STORM) to quantify the colocalization of the flagellin-sensitive-2 (FLS2) receptor and the nanodomain marker, remorin, within Arabidopsis thaliana root hair cells. We found that recovery of FLS2 and remorin in the plasma membrane, following ligand-induced internalization by bacterial-flagellin-peptide (flg22), reached ~85% of their original membrane density after ~90 min. The pairs colocalized at the membrane at greater frequencies, compared with simulated randomly distributed pairs, except for directly after recovery, suggesting initial uncoordinated recovery followed by remorin and FLS2 pairing in the membrane. The purinergic receptor, P2K1, colocalized with remorin at similar frequencies as FLS2, while FLS2 and P2K1 colocalization occurred at significantly lower frequencies, suggesting that these RLKs mostly occupy distinct nanodomains. The chitin elicitor receptor, CERK1, colocalized with FLS2 and remorin at much lower frequencies, suggesting little coordination between CERK1 and FLS2. These findings emphasize STORM’s capacity to observe distinct nanodomains and degrees of coordination between plant cell receptors, and their respective immune pathways.
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Thèses sur le sujet "Nanodomini"

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DITERLIZZI, MARIANNA. « Polymeric Water-Processable Nanoparticles towards sustainable organic photovoltaics ». Doctoral thesis, Università degli Studi di Milano-Bicocca, 2022. http://hdl.handle.net/10281/376407.

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Il mio progetto di dottorato è focalizzato sullo sviluppo di inchiostri a base acquosa costituiti da nanoparticelle (NPs) polimeriche per applicazioni optoelettroniche ed elettroniche. In particolare, lo scopo della mia ricerca è la preparazione di strati attivi di dispositivi fotovoltaici organici (OPV) sostenibili. Questo obiettivo è raggiunto attraverso sospensioni acquose di NPs processabili in acqua, preparate a partire da miscele di polimeri semiconduttori donatori e accettori di elettroni. Gli inchiostri acquosi sono stati ottenuti attraverso il metodo della miniemulsione, modificato affinché non fosse necessaria l'aggiunta di surfattanti per garantire la stabilità colloidale. L'approccio sviluppato prevede l'utilizzo di copolimeri a blocchi (BCPs) anfifilici del tipo rod-coil, caratterizzati da un blocco rigido (un polimero semiconduttore di tipo p) legato covalentemente a un segmento flessibile idrofilo in grado di interagire con il mezzo acquoso, stabilizzando le interfacce acquose/non acquose. I BCPs anfifilici sono in grado di auto-assemblarsi sia puri che in miscela con materiali accettori, portando alla formazione di nanostrutture costituite da domini con dimensioni adatte alla percolazione della carica nello strato attivo della cella solare organica (OSC). In primo luogo, come materiali elettron-donatori sono stati considerati dei polimeri low band-gap (LBG). Nella parte iniziale della tesi è descritta la sintesi di quattro BCPs basati sul polimero poli[2,6-(4,4-bis-(2-etilesil)-4H-ciclopenta[2,1-b;3,4-b']ditiofene)-alt-4,7(2,1,3-benzotiazolo)] (PCPDTBT), con un segmento di poli-4-vinilpiridina (P4VP) come coil. I BCPs sono stati utilizzati in miscela con il derivato fullerenico PC61BM come materiale elettron-accettore per ottenere inchiostri acquosi, che sono stati poi depositati per fabbricare strati attivi. In seguito, sono stati condotti diversi esperimenti per trovare la correlazione tra la morfologia interna e la composizione delle NPs con l'efficienza dei dispositivi OPV. Successivamente, sono stati studiati altri polimeri LBG dotati di un parziale grado di cristallinità, al fine di migliorare l'efficacia del metodo sviluppato. Pertanto, nella seconda parte della tesi si discute la sintesi e la caratterizzazione di un nuovo BCP anfifilico basato sul poli[[4,8-bis[(2-etilesil)ossi]benzo[1,2-b:4,5-b']ditiofene-2,6-diil][3-fluoro-2-[(2-etilesil)carbonil]tieno[3,4-b]tiofenediil]] (PTB7) come blocco rigido, che è più rigido e più cristallino del PCPDTBT. Come blocco coil è stato scelto un segmento costituito da 15 unità di 4VP. Quindi sono state preparate le NPs tramite self-assembly del PTB7-b-P4VP miscelato con il derivato fullerenico PC71BM. Le sospensioni acquose ottenute sono state impiegate per fabbricare dispositivi OPV in configurazione diretta, e la cella migliore che è stata ottenuta presenta un’efficienza pari a 0.85%, che è un valore ancora molto lontano dal benchmark, ma è comunque superiore all'efficienza del dispositivo ottenuto depositando la miscela PC71BM:PTB7-b-P4VP da solventi alogenati. Infine, è stato preso in considerazione l'uso di tensioattivi nella preparazione delle NPs, in quanto le sospensioni acquose che ne risultano sono più stabili e più facili da maneggiare e conservare, facilitando il processo di scale-up a livello industriale. In quest’ultima parte della tesi, sono stati studiati altri polimeri semiconduttori come materiali elettron-donatori. In particolare, sono stati sintetizzati e caratterizzati due nuovi polimeri semiconduttori LBG e uno a medio band-gap. Questi materiali saranno miscelati con accettori fullerenici e non per ottenere inchiostri a base acquosa che saranno depositati come strati attivi di dispositivi optoelettronici, analogamente a quanto fatto per i materiali precedenti.
My PhD project is focused on the development of polymeric nanoparticle-based aqueous inks for optoelectronic and electronic applications. Specifically, the aim of my research is the fabrication of sustainable active layers of organic photovoltaic (OPV) devices processable in water. This goal is reached through water-processable nanoparticle (WPNP) aqueous suspensions, prepared from semiconducting polymers as electron-donor and acceptor materials. The aqueous inks are obtained through a modified miniemulsion method, which unlike the standard process does not imply the addition of any surfactant to ensure the colloidal stability. The adapted approach involves the use of amphiphilic rod-coil block copolymers (BCPs), characterized by a rigid block (a p‐type semiconducting polymer) covalently linked to a hydrophilic flexible segment able to interact with aqueous medium, stabilizing the aqueous/non-aqueous interfaces. The amphiphilic BCPs are able to self-assemble both neat and in blend with acceptor materials, leading to the formation of nanostructures consisting of domains with dimensions suitable for the charge percolation in the resulting active layer of the organic solar cell (OSC). Primarily, low-band-gap (LBG) polymers were considered as electron donor materials to match the solar radiation absorption. Firstly, the synthesis of four different poly[2,6-(4,4-bis-(2-ethylhexyl)-4H-cyclopenta[2,1-b;3,4-b’]dithiophene)-alt-4,7(2,1,3-benzothiadiazole)] (PCPDTBT)-based amphiphilic BCPs, with a tailored segment of poly-4-vinylpiridine (P4VP) as coil, was presented. The BCPs were used in blend with the [6,6]-phenyl-C61-butyric acid methyl ester (PC61BM) as acceptor material to prepare WPNP aqueous inks, which were deposited to obtain the active layers. The correlation between the internal morphology and composition of the WPNPs, and the dimensions of the donor/acceptor nanodomains with the efficiency of the resulting OSCs was deeply studied. In a second time, we explored other LBG polymers endowed with a partial order to improve the effectiveness of the approach. Therefore, the synthesis and the deep characterization of a new amphiphilic BCP based on the poly[[4,8-bis[(2-ethylhexyl)oxy]benzo[1,2-b:4,5-b’]dithiophene-2,6-diyl][3-fluoro-2-[(2-ethylhexyl)carbonyl]thieno[3,4-b]thiophenediyl]] (PTB7) as rigid donor polymer, which is stiffer and more crystalline than PCPDTBT, were described. A segment of 15 repeating units of 4VP was selected as coil. We prepared WPNPs coming from the self-assembly of the PTB7-b-P4VP blended with the [6,6]-phenyl-C71-butyric acid methyl ester (PC71BM). Subsequently, the WPNPs were employed to fabricate OSCs in direct configuration, and the best gained OPV device exhibited a PCE of 0.85%, which is still very far from the benchmark, but it is higher than the efficiency of the device obtained depositing the PC71BM:PTB7-b-P4VP from halogenated solvents. Lastly, the use of surfactants in the WPNP preparation was considered, as the resulting aqueous suspensions are more stable and easier to handle and store, enhancing the industrial scale-up process. Other semiconducting polymers were selected as electron-donor materials in the active blends. Particularly, two new LBG semiconducting BDT-based polymers, and a medium band-gap one, were synthetized and characterized. These materials will be blended with fullerene and non-fullerene acceptor (NFA) materials to obtain aqueous inks that will be deposited as active layers of optoelectronic devices, similarly to previous materials.
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Imai, Tomoya. « Nanodomain Structure of Native Cellilose Microfibril ». Kyoto University, 2000. http://hdl.handle.net/2433/78103.

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Kyoto University (京都大学)
0048
新制・課程博士
博士(農学)
甲第8426号
農博第1110号
新制||農||800(附属図書館)
学位論文||H12||N3383(農学部図書室)
UT51-2000-F330
京都大学大学院農学研究科森林科学専攻
(主査)教授 伊東 隆夫, 教授 東 順一, 教授 藤田 稔
学位規則第4条第1項該当
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Legrand, Anthony. « Anchoring mechanism of the plant protein remorin to membrane nanodomains ». Thesis, Bordeaux, 2020. http://www.theses.fr/2020BORD0285.

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La rémorine du groupe 1 isoforme 3 de Solanum tuberosum (StREM1.3) est une protéine membranaire de la famille multigénique de protéines de plante appelée rémorines (REMs), impliquées dans l’immunité des plantes, la symbiose, la résistance aux stress abiotiques et la signalisation hormonale. La caractéristique la plus connue des REMs est leur capacité à se ségréger en nanodomaines au feuillet interne de la membrane plasmique (MP). Pour StREM1.3, ceci se fait via une interaction entre deux lysines de l’ancre C-terminale de la rémorine (RemCA) et le phosphatidylinositol 4-phosphate (PI4P) négativement chargé. Ainsi, RemCA modifie sa conformation et s’enfonce partiellement dans la MP, résultant en un accrochage membranaire intrinsèque. Capitalisant sur les données structurales déjà disponibles concernant cet isoforme, nous investiguons StREM1.3 davantage quant à ses propriétés d’interaction membranaire, en utilisant un large éventail de techniques, allant de la microscopie de fluorescence et de la RMN à l’état solide (ssNMR) à la microscopie de force atomique (AFM), la cryo-microscopie électronique (cryoEM) et la modélisation informatique. Nous souhaitons découvrir l’impact de l’oligomérisation et de la phosphorylation de StREM1.3 sur ses interactions membranaires et son activité biologique, ainsi que d’examiner son influence sur la dynamique des lipides et les lipides requis pour l’accrochage à la membrane et le regroupement en nanodomaines. Enfin, forts de toutes les données structurales disponibles, nous entreprendrons la reconstruction in vitro et la caractérisation de nanodomaines minimaux de StREM1.3
Group 1 isoform 3 remorin from Solanum tuberosum (StREM1.3) is a membrane protein belonging to the multigenic family of plant proteins called remorins (REMs), involved in plant immunity, symbiosis, abiotic stress resistance and hormone signalling. REMs’ most well known feature is their ability to segregate into nanodomains at the plasma membrane’s (PM) inner leaflet. For StREM1.3, this is achieved by an interaction between two lysines of the remorin C-terminal anchor (RemCA) and negatively charged phosphatidylinositol 4-phosphate (PI4P). Thus, RemCA undergoes conformational changes and partially buries itself in the PM, resulting in an intrinsic membrane anchoring. Capitalising on pre-existing structural data about this isoform, we investigate StREM1.3’s membrane-interacting properties further, using a wide array of techniques, ranging from fluorescence microscopy and solid-state nuclear magnetic resonance (ssNMR) to atomic force microscopy (AFM), cryo-electron microscopy (cryoEM) and computational modelling. We aim to discover the impact of StREM1.3’s oligomerisation and phosphorylation on its membrane interactions and biological activity, and to assess its influence on lipid dynamics as well as its lipid requirements for membrane binding and nanoclustering. Finally, based on all available structural data, we will undertake the in vitro reconstruction and characterisation of minimal nanodomains of StREM1.3
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Yu, Chao. « Quantitative Study of Membrane Nano-organization by Single Nanoparticle Imaging ». Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLX054.

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La nano-organisation de la membrane cellulaire est essentielle à la régulation de certaines fonctions cellulaires. Dans cette thèse, les récepteurs EGF, CPεT et de la transferrine ont été marqués avec des nanoparticules luminescentes et ont été suivis à la fois dans leur environnement local dans la membrane cellulaire vivantes pour de longues durées et sous un flux hydrodynamique. Nous avons alors appliqué des techniques d'inférence bayésienne, d’arbre de décision et de clustering de données extraire des informations quantitatives sur les paramètres caractéristiques du mouvement des récepteurs, notamment la forme de leur confinement dans des microdomaines. L’application d’une force hydrodynamique sur les nanoparticules nous a alors permis de sonder les interactions auxquelles ces récepteurs sont soumis. Nous avons appliqué cette approche in vitro pour favoriser et mesurer la dissociation in vitro de paires récepteur / ligand à haute affinité entre des récepteurs membranaires et leurs ligands pharmaceutiques, telles que HB-EGF et DTR et l’avons ensuite appliqué à l’étude d’interactions à la membrane cellulaire. Nous avons ainsi mis en évidence trois modes différents d'organisation de la membrane et de confinement des récepteurs: le confinement de CPεTR est déterminé par l'interaction entre les récepteurs et les constituants lipidiques / protéiques des microdomaines, le potentiel de confinement de l'EGFR résulte de l'interaction avec les lipides et les protéines de l’environnement du radeau et de l’interaction avec la F-actine; les récepteurs de la transferrine diffusent librement dans la membrane, uniquement limités stériquement par des barrières d’actine, selon le modèle ‘picket-and-fence’. Nous avons de plus montré que les nanodomaines de type radeau sont rattachés au cytoskelette d’actine. Ce travail présente donc à la fois un aperçu quantitatif du récepteur membranaire, des mécanismes d’organisation à l’échelle nanométrique, et établit un cadre méthodologique avec lequel différents types de propriétés membranaires peuvent être étudiés
In this thesis, EGF, CPεT and transferrin receptors were labeled with luminescent nanoparticles, , and were tracked both in their local environment in the cell membrane and under a hydrodynamic flow. Bayesian inference, Bayesian decision tree, and data clustering techniques can then be applied to obtain quantitative information on the receptor motion parameters. Furthermore, we introduced hydrodynamic force application in vitro to study biomolecule dissociation between membrane receptors and their pharmaceutical ligands in high affinity receptor- ligand pairs, such as HB-EGF and DTR. Finally, three different modes of membrane organization and receptor confinement were revealed: the confinement of CPεTR is determined by the interaction between the receptors and the lipid/protein constituents of the raft; the confining potential of EGFR results from the interaction with lipids and proteins of the raft environment and from the interaction with F-actin; transferrin receptors diffuse freely in the membrane, only sterically limited by actin barriers, according to the “picket-and-fence” model. We moreover showed that all raft nanodomains are attached to the actin cytoskeleton
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Hebisch, Elke [Verfasser], et Stefan W. [Akademischer Betreuer] Hell. « STED microscopy of cardiac membrane nanodomains / Elke Hebisch ; Betreuer : Stefan W. Hell ». Heidelberg : Universitätsbibliothek Heidelberg, 2017. http://d-nb.info/1180740068/34.

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Hebisch, Elke [Verfasser], et Stefan [Akademischer Betreuer] Hell. « STED microscopy of cardiac membrane nanodomains / Elke Hebisch ; Betreuer : Stefan W. Hell ». Heidelberg : Universitätsbibliothek Heidelberg, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:16-heidok-227475.

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Liu, Xian-He. « Perfluoroalkylated compounds at the Interfaces : surface nanodomains and spherulites : interactions with phospholipid films ». Thesis, Strasbourg, 2018. http://www.theses.fr/2018STRAF029.

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Cette thèse concerne l’auto-assemblage d'alcanes semi-fluorés (FnHm) et de fluorocarbures (FCs) aux interfaces et leurs interactions avec des phospholipides (PLs) en 2D et 3D. Nous avons étudié les sphérulites formées dans des films de diblocs FnHm. La morphologie de ces sphérulites, concentrique ou radiale, est contrôlée par la longueur des blocs Fn et Hm et la vitesse de refroidissement. Les nanodomaines de diblocs FnHm dans les monocouches de Langmuir sont incompressibles et forment des gels physiques 2D, même à pression nulle. Les blocs Hm sont cristallisés et inclinés d’ ~30°C par rapport à la normale à la surface. La réflectivité de neutrons a montré que l’albumine adsorbée sur des monocouches de PL est désorbée par un FC gazeux. Ce résultat pourrait permettre de lutter contre l’inactivation du surfactant pulmonaire par les protéines sériques. L’ajout de diblocs à des films de PLs accroit l'élasticité des monocouches. Nous avons préparé des microbulles stables à parois de PLs/FnHm, les diblocs agissant en co-surfactants
This thesis focuses on the self-assembly of semi-fluorinated alkanes (FnHm) and fluorocarbons (FCs) at interfaces and their interactions with phospholipids (PLs) in 2D and 3D. 2D Spherulites were identified in FnHm films for the first time. Their morphology, ring-banded or radial, was controlled by varying block lengths and cooling rate. Nanodomains of FnHm in monolayers formed incompressible 2D physical gels, even at zero surface pressure. The Hm segments are crystalline and titled by 30°C to the normal to the surface. Neutron reflectivity showed that albumin adsorbed on PLs monolayers is desorbed by exposure to FC gas, which opens the potential use of FCs to treat the inactivation of the lung surfactant by serum proteins. Incorporating FnHm into PL monolayers increases their elasticity. Small, stable microbubbles of PLs/FnHm were obtained. FnHm diblocks function as co-surfactants for stabilizing microbubbles
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Parutto, Pierre. « Statistical analysis of single particle trajectories reveals sub-cellular nanodomain organisation and function ». Thesis, Paris Sciences et Lettres (ComUE), 2019. http://www.theses.fr/2019PSLEE055.

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Les trajectoires de molécules individuelles obtenues par microscopie super-résolution permettent de suivre des protéines avec une précision nanométrique dans des cellules vivantes. Dans cette thèse, j’ai étudié les régions de hautes densités présentes dans ces trajectoires, dont un modèle possible est celui des puits de potentiel. Pour les caractériser à partir de trajectoires, j’ai développé une nouvelle méthode hybride basée sur la densité de points et le champ de force local puis je l’ai comparé aux méthodes d’état de l’art. Ensuite, j’ai utilisé celle-ci pour caractériser les puits maintenant les canaux calciques Cav au niveau des zones actives des terminaux présynaptiques ce qui a permis de mieux comprendre le rôle des variantes d’épissage de ces canaux dans la transmission synaptique. Dans une autre étude, j’ai analysé des trajectoires de protéines résidant dans le lumen du Réticulum Endoplasmique (RE). J’ai créé une méthode pour reconstruire le réseau du RE à partir des trajectoires que j’ai utilisé pour caractériser le mouvement de ces molécules par un modèle de saut-diffusion qui a pour conséquence une meilleure redistribution du contenu luminal par rapport à un mouvement diffusif. Enfin, je discute d’autres analyses de trajectoires pour les intéractions lysosome-ER, les canaux Cav à la jonction neuro-musculaire de la drosophile et les protéines composant le complexe NuRD
Single-Particle Trajectories (SPTs) obtained from super-resolution microscopy allow to track proteins with nanometer precision in living cells and are used in neuroscience and cellular biology. In this thesis, I was interested in the high-density nanodomains found in these trajectories that can be modeled as potential wells. To characterize them, I developed a new hybrid method based on the point density and local drift field and compared it to the other state-of-the-art methods. Then, I used it to identify transient potential wells in SPTs of voltage-gated calcium channels (CaV) contributing to a better understanding of the role of the different CaV splice variants in synaptic transmission. In another study, I looked at SPTs from Endoplasmic Reticulum (ER) luminal resident proteins where I developed a method to reconstruct the network from trajectories and used it to characterize the luminal motion as a jump-diffusion process, which allows for a better redistribution of the luminal content than the previously assumed diffusive model. Finally, I discuss other analyses of motions for lysosome-ER interactions, CaV2.1 channels at drosophila’s neuromuscular junctions and the description of the motion of the constituent proteins of the NuRD chromatin remodeling complex
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Sachl, Radek. « Localisation of Fluorescent Probes and the estimation of Lipid Nanodomain sizes by modern fluorescence techniques ». Doctoral thesis, Umeå universitet, Kemiska institutionen, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-52619.

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The thesis is divided into two major parts. The first part focuses on the localisation of probes in lipid/polymeric bilayers and in GM1 micelles. Included in this thesis is a new approach based on electronic energy transfer/migration (FRET/DDEM), which efficiently determines transversal positions of fluorescent molecules in lipid bilayers. This approach has been used to locate newly synthesized lipid probes in DOPC bilayers. The label was introduced at the end of sn-2 acyl chains of variable length. Analytical models accounting for FRET exist for a limited number of basic geometries. Here, a combination of FRET and Monte Carlo simulations enables the localisation of probes in bicelles and in bilayers containing pores, i.e. in lipid systems with variable curvature, or in non-homogenous lipid systems. This approach has been used to test whether conical-like fluorescence probes have an increased affinity to highly curved regions, which would enable preferential labelling of membrane pores. A simplified FRET model has been applied to localize 2-pyridones, a class of potential drugs, in GM1 micelles. Since the localisation of drugs within nanoparticles might influence the release kinetics and loading efficiency, knowledge about the drug location is highly relevant. It turned out that all derivatives were localised at the core-shell interface of GM1 micelles. The second part of the thesis focuses mainly on the estimation of lipid nanodomain size by means of FRET, which still remains the most powerful method in this field. Limitations of FRET in the determination of domain size have been explored. We showed that the limitations of FRET are mainly caused by a low probes affinity to either the liquid-ordered or liquid-disordered phase. In the continuing work we provided a detailed dynamic and structural study of crosslinker-triggered formation of nanodomains. Here, two different domains have been revealed, i.e. i) domains whose size grows with increasing amount of added cholera toxin (CTxB), and to which CTxB binds tightly; ii) domains formed in membranes containing a slightly increased amount of sphingomyelin (as compared to i) whose size does not change during titration by additional CTxB and to which CTxB binds less tightly.
Disertace je rozdělena do dvou hlavníchčástí. Prvníčást se zabývá lokalizací značek v lipidových/polymerních dvojvrstvách a v GM1micelách. V práci prezentujeme nový přístup založený na přenosu/migraci elektronické energie (FRET/DDEM), jež umožňuje efektivně určovat vertikální pozici fluorescenčních molekul uvnitř lipidové dvojvrstvy. Tato metoda byla použita k lokalizaci nově syntetizovaných lipidových značek značených na konci sn-2 acylového řetězce s různou délkou v DOPC dvojvrstvách. Analytické modely popisující FRET existují pouze pro limitovaný počet základních geometrií. Kombinace FRETu s Monte Carlo simulacemi nicméně umožňuje lokalizaci značek v bicelách a v dvojvrstvách obsahujících póry, tj. v lipidových systémech s proměnlivým zakřivením a v nehomogenních lipidových útvarech. Tento přístup umožnil např. zjistit, zda kuželovitětvarované značky mají zvýšenou afinitu k vysoce zakřiveným oblastem dvojvrstvy, což by umožnilo preferenční značení pórů. Lokalizovány byly rovněž tři deriváty 2-pyridonů(potencionálních léčiv) v GM1micelách za použití jednoduchého modelu zohledňujícího FRET mezi donory a akceptory nacházejícími se v micelách. Lokalizace léčiv v nanočásticích ovlivňuje kinetiku uvolňování (release kinetics) a množství látky solubilizované v micelách (loading efficiency). Druhá část se především zabývá určováním velikostí lipidových nanodomén pomocí FRETu, který stále zůstává nejvíce výkonnou metodou v této oblasti. Zkoumány byly limitace FRETu v určování lipidových nanodomén. Ukázalo se, že tato omezení jsou především způsobena nízkou afinitou značek buď k Lonebo k Ldfázi. V navazující studii jsme poskytnuli detailní dynamickou a strukturní studii formace nanodomén indukované crosslinkerem. Objevili jsme dva typy domén: a) domény, jejichž velikost se zvětšuje s rostoucím množstvím přidaného cholera toxinu (CTxB) a k nimž se CTxB váže pevně a b) domény vzniklé v membránách se zvýšeným množstvím sfingomyelinu (ve srovnání s a)), jejichž velikost se nemění během titrace dodatečným CTxB a k nimž se CTxB váže méně pevně.
This thesis has been elaborated within the framework of the Agreement on JointSupervision (co-tutelle) of an International Doctoral Degree Programmebetween Charles University in Prague, Czech Republic and the Department of Chemistry at Umeå University, Sweden.
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Kirsch, Sonja [Verfasser], Rainer [Akademischer Betreuer] Böckmann et Rainer [Gutachter] Böckmann. « The Role of Membrane Nanodomains in Permeation / Sonja Kirsch ; Gutachter : Rainer Böckmann ; Betreuer : Rainer Böckmann ». Erlangen : Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 2019. http://d-nb.info/1196875901/34.

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Livres sur le sujet "Nanodomini"

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Silvius, John R. Membrane Nanodomains. Morgan & Claypool Life Science Publishers, 2013.

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Silvius, John R. Membrane Nanodomains. Morgan & Claypool Life Science Publishers, 2013.

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Cambi, Alessandra, et Diane Lidke. Cell Membrane Nanodomains. Taylor & Francis Group, 2014.

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Cambi, Alessandra, et Diane Lidke. Cell Membrane Nanodomains. Taylor & Francis Group, 2021.

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Cell Membrane Nanodomains : From Biochemistry to Nanoscopy. Taylor & Francis Group, 2014.

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Cambi, Alessandra, et Diane S. Lidke. Cell Membrane Nanodomains : From Biochemistry to Nanoscopy. Taylor & Francis Group, 2014.

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Cambi, Alessandra, et Diane S. Lidke. Cell Membrane Nanodomains : From Biochemistry to Nanoscopy. Taylor & Francis Group, 2014.

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Chapitres de livres sur le sujet "Nanodomini"

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Srivastava, Shilpi, et Atul Bhargava. « The Nanodomain ». Dans Green Nanoparticles : The Future of Nanobiotechnology, 15–28. Singapore : Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-7106-7_2.

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Fridkin, Vladimir, et Stephen Ducharme. « Ferroelectric Nanocrystals and Nanodomains ». Dans Ferroelectricity at the Nanoscale, 67–85. Berlin, Heidelberg : Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-41007-9_5.

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Shi, An-Chang. « Nanodomain Structure in Block/Graft Copolymers ». Dans Encyclopedia of Polymeric Nanomaterials, 1–5. Berlin, Heidelberg : Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-36199-9_59-1.

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Shi, An-Chang. « Nanodomain Structure in Block/Graft Copolymers ». Dans Encyclopedia of Polymeric Nanomaterials, 1317–20. Berlin, Heidelberg : Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-642-29648-2_59.

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Rosenman, G., A. Agronin, D. Dahan, M. Shvebelman, E. Weinbrandt, M. Molotskii et Y. Rosenwaks. « Ferroelectric Domain Breakdown : Application to Nanodomain Technology ». Dans Polar Oxides, 189–220. Weinheim, FRG : Wiley-VCH Verlag GmbH & Co. KGaA, 2005. http://dx.doi.org/10.1002/3527604650.ch10.

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Guyonnet, Jill. « Disorder and Environmental Effects on Nanodomain Growth ». Dans Springer Theses, 133–43. Cham : Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-05750-7_9.

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Betker, Jamie L., Long Xu, Ye Zhang et Thomas J. Anchordoquy. « Utilizing Cholesterol Nanodomains for Nucleic Acid Delivery ». Dans ACS Symposium Series, 71–93. Washington, DC : American Chemical Society, 2017. http://dx.doi.org/10.1021/bk-2017-1271.ch003.

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Wang, Biao. « Determination of the Smallest Sizes of Ferroelectric Nanodomains ». Dans Advanced Topics in Science and Technology in China, 147–77. Berlin, Heidelberg : Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-33596-9_4.

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Ganesan, Sai J., Hongcheng Xu et Silvina Matysiak. « Cation-Mediated Nanodomain Formation in Mixed Lipid Bilayers ». Dans Biomembrane Simulations, 199–212. Boca Raton, FL : CRC Press, Taylor & Francis Group, [2019] | : CRC Press, 2019. http://dx.doi.org/10.1201/9781351060318-11.

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Vakulenko, Alexandr, Natalia Andreeva, Sergej Vakhrushev, Alexandr Fotiadi et Alexey Filimonov. « Writing Ferroelectric Nanodomains in PZT Thin Film at Low Temperatures ». Dans Lecture Notes in Computer Science, 708–16. Cham : Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-46301-8_62.

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Actes de conférences sur le sujet "Nanodomini"

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Chirasatitsin, Somyot, Priyalakshmi Viswanathan, Giuseppe Battaglia et Adam J. Engler. « Directing Stem Cell Fate in 3D Through Cell Inert and Adhesive Diblock Copolymer Domains ». Dans ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14442.

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Adhesions are important cell structures required to transduce a variety of chemical and mechanics signals from outside-in and vice versa, all of which regulate cell behaviors, including stem cell differentiation (1). Though most biomaterials are coated with an adhesive ligand to promote adhesion, they do not often have a uniform distribution that does not match the heterogeneously adhesive extracellular matrix (ECM) in vivo (2). We have previously shown that diblock copolymer (DBC) mixtures undergo interface-confined de-mixing to form nanodomins of one copolymer in another (3). Here we demonstrate how diblock copolymer mixtures can be made into foams with nanodomains to better recapitulate native ECM adhesion regions and influence cell adhesion.
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Vakhrushev, S. B. « Structure of Nanodomains in Relaxors ». Dans Fundamental Physics of Ferroelectrics 2003. AIP, 2003. http://dx.doi.org/10.1063/1.1609940.

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Forget, G., L. Latxague, V. Heroguez, C. Labrugere et M. C. Durrieu. « RGD nanodomains grafting onto titanium surface ». Dans 2007 29th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 2007. http://dx.doi.org/10.1109/iembs.2007.4353489.

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Tkachuk, A. « Anti-ferrodistortive Nanodomains in PMN Relaxor ». Dans Fundamental Physics of Ferroelectrics 2003. AIP, 2003. http://dx.doi.org/10.1063/1.1609938.

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Rong, Xi, Kenneth M. Pryse, Jordan A. Whisler, Yanfei Jiang, William B. McConnaughey, Artem Melnykov, Guy M. Genin et Elliot L. Elson. « Confidence Intervals for Estimation of the Concentration and Brightness of Multiple Diffusing Species ». Dans ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80921.

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Lipid nanodomains in cell membranes are believed to play a significant role in replication of enveloped viruses such as bird flu and HIV and signaling mechanisms underlying pathological conditions such as cancer. However, the forces that govern the formation and availability of these “membrane rafts” are uncertain, and even their existence is questioned. The central challenge is that no suitable imaging modalities exist (Elson, et al., 2010). We are developing tools to characterize and visualize dynamics of lipid nanodomains on idealized systems called giant unilamellar vesicles (GUVs) using fluorescence correlation spectroscopy (FCS) (Figure 1).
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Gentilini, Desiree, Daniele Rossi, Matthias Auf der Maur, Aldo Di Carlo et Alessandro Pecchia. « Effect of ferroelectric nanodomains in perovskite solar cells ». Dans 2015 IEEE 15th International Conference on Nanotechnology (IEEE-NANO). IEEE, 2015. http://dx.doi.org/10.1109/nano.2015.7388894.

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Noheda, B., A. Vlooswijk, A. Janssens, G. Catalan, G. Rijnders et D. H. A. Blank. « Periodic nanodomains in PbTiO3 films under tensile strain ». Dans 2008 17th IEEE International Symposium on the Applications of Ferroelectrics (ISAF). IEEE, 2008. http://dx.doi.org/10.1109/isaf.2008.4693859.

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Kurushima, K., K. Kobayashi et S. Mori. « Nanodomain structures with hierarchical inhomogeneities in PMN-PT ». Dans Nanoscale Phenomena in Polar Materials. IEEE, 2011. http://dx.doi.org/10.1109/isaf.2011.6014143.

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Ignatans, Reinis. « Temperature-and voltage-dependent nanodomain dynamics in BaTiO3 ». Dans European Microscopy Congress 2020. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.emc2020.980.

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Hussey, Deborah M., Lukas Keller, Nathan A. Diachun, Andy H. Marcus et Michael D. Fayer. « Nanodomain formation and phase separation in polymer blends ». Dans Photonics West '96, sous la direction de E. R. Menzel et Abraham Katzir. SPIE, 1996. http://dx.doi.org/10.1117/12.236183.

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