Articles de revues : « MPSI »

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Sornalingam, Krishanthy, Ahmed Javed, Tariq Aslam, Panagiotis Sergouniotis, Simon Jones, Arunabha Ghosh et Jane Ashworth. « Variability in the ocular phenotype in mucopolysaccharidosis ». British Journal of Ophthalmology 103, n^o 4 (17 août 2018) : 504–10. http://dx.doi.org/10.1136/bjophthalmol-2017-311749.

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PurposeMucopolysaccharidoses (MPSs) are a heterogeneous group of lysosomal storage disorders. Ocular complications (such as corneal clouding, retinopathy and optic neuropathy) are common. Notably, there is a paucity of data on the effect of genotype and systemic treatments (enzyme replacement therapy or haematopoietic stem cell transplantation) on the ocular phenotype in MPS. We prospectively studied the ocular features of patients with MPSI (Hurler/Hurler-Scheie/Scheie), MPSIV (Morquio) and MPSVI (Maroteaux-Lamy), to evaluate the effect of different therapeutic interventions and to correlate the findings with genetic and biomarker data.MethodsProspective observational cohort study. Study participants underwent detailed ocular examination including visual acuity; assessment of corneal clouding (Iris camera Corneal Opacification Measure score and Pentacam densitometry) and retinal and optic nerve imaging (optical coherence tomography and wide-field fundus imaging). Data on genotype, biomarkers and delivered therapies (type and length of treatment) were also collected for each patient where available.ResultsOverall, 21 patients with MPSI, 4 patients with MPSIV and 3 patients with MPSVI were recruited. Corneal clouding scores were higher in MPSI compared with MPSIV and MPSVI. Retinopathy was evident in patients with MPSI only. Association was observed between corneal clouding and biomarkers in MPSI, MPSIV and MPSVI. However, no clear association was seen between genotype or treatment type and ocular phenotype.ConclusionsThe ocular phenotype in MPS is variable, with corneal clouding occurring in MPSI, MPSIV and MPSVI, and retinopathy in MPSI only. There was an association between corneal clouding and efficacy of systemic treatment as measured by biomarkers.

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Eremina, A. S., A. Yu Kharin, Yu V. Kargina et V. Yu Timoshenko. « Stabilization of porous silicon nanoparticles by PEGalization in water ». Journal of Physics : Conference Series 2058, n^o 1 (1 octobre 2021) : 012013. http://dx.doi.org/10.1088/1742-6596/2058/1/012013.

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Abstract Mesoporous silicon (mPSi) nanoparticles (NPs) are stabilized by polyethylene glycol (PEG) chains during mechanical grinding in a ball mill that is used to form mPSi-PEG-NPs. The structure, composition, and properties of the obtained samples are studied by means of the dynamic light scattering, scanning electron microscopy and Fourier-transform infrared spectroscopy. The proposed PEGalization procedure is an effective way of regulating the dissolution of mPSi-NPs in water and it is promising for potential application of mPSi-NPs in drug delivery.

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De Ponti, Giada, Samantha Donsante, Marta Frigeni, Alice Pievani, Alessandro Corsi, Maria Ester Bernardo, Mara Riminucci et Marta Serafini. « MPSI Manifestations and Treatment Outcome : Skeletal Focus ». International Journal of Molecular Sciences 23, n^o 19 (22 septembre 2022) : 11168. http://dx.doi.org/10.3390/ijms231911168.

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Mucopolysaccharidosis type I (MPSI) (OMIM #252800) is an autosomal recessive disorder caused by pathogenic variants in the IDUA gene encoding for the lysosomal alpha-L-iduronidase enzyme. The deficiency of this enzyme causes systemic accumulation of glycosaminoglycans (GAGs). Although disease manifestations are typically not apparent at birth, they can present early in life, are progressive, and include a wide spectrum of phenotypic findings. Among these, the storage of GAGs within the lysosomes disrupts cell function and metabolism in the cartilage, thus impairing normal bone development and ossification. Skeletal manifestations of MPSI are often refractory to treatment and severely affect patients’ quality of life. This review discusses the pathological and molecular processes leading to impaired endochondral ossification in MPSI patients and the limitations of current therapeutic approaches. Understanding the underlying mechanisms responsible for the skeletal phenotype in MPSI patients is crucial, as it could lead to the development of new therapeutic strategies targeting the skeletal abnormalities of MPSI in the early stages of the disease.

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Woloszynek, Josh C., Trey Coleman, Clay F. Semenkovich et Mark S. Sands. « Lysosomal Dysfunction Results in Altered Energy Balance ». Journal of Biological Chemistry 282, n^o 49 (2 octobre 2007) : 35765–71. http://dx.doi.org/10.1074/jbc.m705124200.

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The mucopolysaccharidosis (MPS) type VII mouse was originally described as the adipose storage deficiency mouse because of its extreme lean phenotype of unknown etiology. Here, we show that adipose storage deficiency and lower leptin levels are common to five different lysosomal storage diseases (LSDs): MPSI, MPSIIIB, MPSVII, Niemann-Pick type A/B, and infantile neuronal ceroid lipofuscinosis. Elevated circulating pro-inflammatory proteins (VCAM1 and MCP1) were found in multiple LSDs. Multiple anti-inflammatory strategies (dexamethasone, MCP1 deficiency, M3 expression) failed to alter adiposity in LSD animals. All of the models had normal or greater caloric intake and lower to normal metabolic rate, fasting plasma glucose, non-esterified fatty acids, cholesterol, and triglycerides. Triglycerides were lower in the livers of MPSI mice, and the trend was lower in the muscle. Lipid absorption and processing in MPSI mice were indistinguishable from those in normal mice following oral gavage of olive oil. The increased lean mass of MPSI and MPSIIIB mice suggests a shift in adipose triglycerides to lysosomal storage. In agreement, MPSI livers had a similar total caloric content but reduced caloric density, indicating a shift in energy from lipids to proteins/carbohydrates (lysosomal storage). Enzyme replacement therapy normalized the caloric density within 48 h without reducing total caloric content. This was due to an increase in lipids. Recycling of stored material is likely reduced or nonexistent. Therefore, to maintain homeostasis, energy is likely diverted to synthesis at the expense of typical energy storage depots. Thus, these diseases will serve as important tools in studying the role of lysosome function in metabolism and obesity.

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Sunardi, Bambang, Sulastri, Dwikorita Karnawati, Urip Haryoko, Supriyanto Rohadi, Sigit Pramono et Ari Sungkowo. « Acceleration Response Spectra for M 7.4 Donggala Earthquake and Comparison with Design Spectra ». Journal of Sustainable Engineering : Proceedings Series 1, n^o 1 (30 juin 2019) : 20–26. http://dx.doi.org/10.35793/joseps.v1i1.3.

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A 7.4 magnitude earthquake have strucked Donggala on September 28th 2018, followed by tsunami and liquefaction which hit Palu, Central Sulawesi, a few minutes later. This event had resulted in damage to buildings, and caused more than 2,000 people were killed and injured. Indonesia already have a building code in form of SNI 1726:2002 which had been updated to SNI 1726:2012. This paper analyses the hazard level caused by the 2018 Donggala earthquake compared to the existing design spectra, as mentioned in SNI 1726:2002 and SNI 1726:2012. A simple analysis was carried out by comparing Donggala earthquake’s acceleration response spectra with the existing design spectra, at the MPSI accelerograph station. The site class at MPSI station is hard soil (SC). The seismic hazard in Palu and Donggala refers to SNI 1726:2002 is included in the earthquake area 4. The maximum earthquake response factor for earthquake area 4 is about 0.6 for hard soil type (SC). The MPSI station recorded peak ground acceleration of Donggala earthquake around 0.14 g. The acceleration response spectra recorded at the MPSI station showed a peak value of around 0.71 g for the N component. This value is actually still below the design spectra referring to SNI 1726:2012, which the peak value is 0.88 g for SC, but, it exceeded the design spectra of SNI 1726:2002.

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Pei, Song, Xingyao Yin et Kun Li. « Full-time domain matching pursuit and empirical mode decomposition based sparse fixed-point seismic inversion ». Journal of Geophysics and Engineering 19, n^o 2 (avril 2022) : 255–68. http://dx.doi.org/10.1093/jge/gxac014.

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Abstract Sparse seismic inversion is wildly utilized for reservoir prediction and resolution improvement. Matching pursuit (MP) is an effective algorithm for solving L0-norm and obtaining sparse inversion results. Sparse seismic inversion based on MP (MPSI) can estimate the sparse parameters of subsurface from observations by controlling the iterations and threshold. However, the low convergence and stability limit the application of MPSI. To accelerate the convergence of MP, the full-time domain matching pursuit (FTMP) algorithm is first proposed. The seismic inversion based on FTMP can realize the multi-point inversion simultaneously instead of searching the inversion results one by one, which is the process of MPSI. Also, the prior model constraint is then involved in the objective function to improve the stability and the layer-boundary fidelity of the inversion results. Furthermore, the empirical mode decomposition (EMD) algorithm is introduced into inversion framework to recover the features of the seismic signal from the noisy seismic signal. The fixed-point (FP) algorithm is adopted to solve the objective function in this study. The optimal inversion results can be estimated after finite iterations by the FP algorithm. Combining the FTMP sparse seismic inversion framework, prior model constraint, EMD and FP algorithms, a complete sparse seismic inversion method named the full-time domain matching pursuit-based sparse fixed-point seismic inversion is ultimately proposed. The synthetic and field examples are utilized to demonstrate the stability and practicality of this approach. Compared with MPSI, the inversion results of the proposed method have higher resolution and fidelity of the layer-boundary.

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Hudson, Walter W., et Steven L. McMurtry. « Comprehensive Assessment in Social Work Practice : The Multi-Problem Screening Inventory ». Research on Social Work Practice 7, n^o 1 (janvier 1997) : 79–98. http://dx.doi.org/10.1177/104973159700700105.

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Findings are reported from an initial evaluation of a new multidimensional assessment tool, the Multi-Problem Screening Inventory (MPSI). The inventory gathers information on 27 different areas of personal and social functioning and is designed for use by human service practitioners in a variety of settings. Basic guidelines for using the instrument are detailed, with emphasis given to the preparation and interpretation of graphic profiles for rapid but comprehensive client assessments. Reliabilities and validities obtained for each of the subscales are then reviewed, along with suggestions for further development and use of the MPSI in clinical trials and research applications.

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Ames, Elizabeth G., Rachel Fisher, Mary Kleyn et Ayesha Ahmad. « Current Practices for U.S. Newborn Screening of Pompe Disease and MPSI ». International Journal of Neonatal Screening 6, n^o 3 (2 septembre 2020) : 72. http://dx.doi.org/10.3390/ijns6030072.

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Two lysosomal storage disorders (LSDs), Pompe disease and Mucopolysaccharidosis type I (MPSI) were added to the Recommended Uniform Screening Panel (RUSP) for newborn screening (NBS) in 2015 and 2016, respectively. These conditions are being screened with variable practice in terms of primary and reflex analytes (either biochemical or molecular testing) as well as collection of short- and long-term follow-up elements. The goal of this study is to evaluate practices of state health departments in regards to screening methods and follow-up data collected. We conducted online surveys and phone questionnaires to determine each U.S. state’s practices for screening and follow-up of positive newborn screens. We report the first snapshot of practices for NBS for the LSDs included on the RUSP. All 50 U.S. states responded to our survey. The majority of U.S. states are not currently screening for Pompe disease and MPSI as of March 2020, but this number will increase to 38 states in the coming 1–3 years based on survey results. Our survey identifies data elements used by state health departments for short-and long-term follow-up that could serve as the basis of common elements for larger, public health-based analyses of the benefits and efficacy of screening for Pompe disease and MPSI.

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Vashakmadze, Nato, Natalya Zhurkova, Ekaterina Zakharova, Marina Babaikina, Anastasia Rykunova et Leyla Namazova-Baranova. « Mild forms of mucopolysaccharidosis type I (MPSI) ». Molecular Genetics and Metabolism 132, n^o 2 (février 2021) : S108. http://dx.doi.org/10.1016/j.ymgme.2020.12.265.

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Kassisse, Elias, Joanna Jimenez, Nelly Mayo et Jorge Kassisse Limongi. « Sulfato de magnesio vs aminofilina como segunda línea de manejo en niños con asma aguda severa. Ensayo clínico aleatorizado ». Andes Pediatrica 92, n^o 3 (22 juin 2021) : 367. http://dx.doi.org/10.32641/andespediatr.v92i3.2969.

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Los medicamentos de segunda línea en el asma aguda generalmente son de administración endovenosa: salbutamol, sulfato de magnesio y aminofilina.Objetivo: comparar la eficacia y seguridad del uso de sulfato de magnesio vs aminofilina en niños que no respondieron al tratamiento inicial.Pacientes y Método: Ensayo clínico aleatorizado. Los niños que no mejoraron el Score de Índice Pulmonar Modificado (mPSI) fueron aleatorizados a recibir sulfato de magnesio (50 mg/Kg/dosis única) o aminofilina (5 mg/Kg/dosis seguida de infusión continua a 1 mg/kg/hora por 3 h). Resultados primarios: cambios en el mPSI y la saturación de oxígeno; resultados secundarios: tasa de hospitalización, necesidad de traslado a unidad de cuidados intensivos, uso de una tercera intervención y efectos adversos.Resultados: Se estudiaron 131 pacientes (66 pacientes en el grupo de la aminofilina y 65 MgSO4). La edad promedio fue de 5 ± 2,3 años, los parámetros demográficos y clínicos no difirieron entre los grupos. En el grupo que recibió sulfato el mPSI y la saturación de oxigeno se modificaron favorablemente de forma significativa desde 13,1 ± 1,3 hasta 4,9 ± 2,5 (p < 0,001) y de 3,3 ± 2,5; (p 0,021), respectivamente. De igual forma en el grupo que recibió el sulfato se redujo el riesgo de admisión (RR 0,68 95% IC [0,56; 0,82]) y el de fallas secundaria (0,16 95% IC 95% [0,07; 0,38]). Solo se registró un evento adverso (taquicardia).Conclusión: La administración de una dosis única de sulfato de magnesio demostró ser más eficaz y segura que el uso de la aminofilina como agente de segunda línea.

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Adams, W. W., et S. J. Krause. « Morphology of High-Performance Rigid-Rod Polymer Fibers and Molecular Composites ». Proceedings, annual meeting, Electron Microscopy Society of America 47 (6 août 1989) : 338–39. http://dx.doi.org/10.1017/s042482010015366x.

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Rigid-rod polymers such as PBO, poly(paraphenylene benzobisoxazole), Figure 1a, are now in commercial development for use as high-performance fibers and for reinforcement at the molecular level in molecular composites. Spinning of liquid crystalline polyphosphoric acid solutions of PBO, followed by washing, drying, and tension heat treatment produces fibers which have the following properties: density of 1.59 g/cm3; tensile strength of 820 kpsi; tensile modulus of 52 Mpsi; compressive strength of 50 kpsi; they are electrically insulating; they do not absorb moisture; and they are insensitive to radiation, including ultraviolet. Since the chain modulus of PBO is estimated to be 730 GPa, the high stiffness also affords the opportunity to reinforce a flexible coil polymer at the molecular level, in analogy to a chopped fiber reinforced composite. The objectives of the molecular composite concept are to eliminate the thermal expansion coefficient mismatch between the fiber and the matrix, as occurs in conventional composites, to eliminate the interface between the fiber and the matrix, and, hopefully, to obtain synergistic effects from the exceptional stiffness of the rigid-rod molecule. These expectations have been confirmed in the case of blending rigid-rod PBZT, poly(paraphenylene benzobisthiazole), Figure 1b, with stiff-chain ABPBI, poly 2,5(6) benzimidazole, Fig. 1c A film with 30% PBZT/70% ABPBI had tensile strength 190 kpsi and tensile modulus of 13 Mpsi when solution spun from a 3% methane sulfonic acid solution into a film. The modulus, as predicted by rule of mixtures, for a film with this composition and with planar isotropic orientation, should be 16 Mpsi. The experimental value is 80% of the theoretical value indicating that the concept of a molecular composite is valid.

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Zheng, Yun, Sigfrid D. Soli, Kai Wang, Juan Meng, Zhaoli Meng, Ke Xu et Yong Tao. « Development of the Mandarin pediatric speech intelligibility (MPSI) test ». International Journal of Audiology 48, n^o 10 (janvier 2009) : 718–28. http://dx.doi.org/10.1080/14992020902902658.

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Nugent, William R. « A Psychometric Study of the MPSI Suicidal Thoughts Subscale ». Stress, Trauma, and Crisis 9, n^o 1 (janvier 2006) : 1–15. http://dx.doi.org/10.1080/15434610500506209.

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Gligorić, Miloš, Zoran Gligorić, Suzana Lutovac, Milanka Negovanović et Zlatko Langović. « Novel Hybrid MPSI–MARA Decision-Making Model for Support System Selection in an Underground Mine ». Systems 10, n^o 6 (9 décembre 2022) : 248. http://dx.doi.org/10.3390/systems10060248.

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An underground mine is a very complex production system within the mining industry. Building up the underground mine development system is closely related to the installation of support needed to provide the stability of mine openings. The selection of the type of support system is recognized as a very hard problem and multi-criteria decision making can be a very useful tool to solve it. In this paper we developed a methodology that helps mining engineers to select the appropriate support system with respect to geological conditions and technological requirements. Accordingly, we present a novel hybrid model that integrates the two following decision-making components. First, this study suggests a new approach for calculating the weights of criteria in an objective way named the Modified Preference Selection Index (MPSI) method. Second, the Magnitude of the Area for the Ranking of Alternatives (MARA) method is proposed as a novel multi-criteria decision-making technique for establishing the final rank of alternatives. The model is tested on a hypothetical example. Comparative analysis confirms that the new proposed MPSI–MARA model is a very useful and effective tool for solving different MCDM problems.

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Rodríguez-Arteche, Iñigo, et M. Mercedes Martínez-Aznar. « Chemistry open-ended problem solving during initial secondary education teacher training ». International Journal of Learning and Teaching 8, n^o 3 (31 octobre 2016) : 174. http://dx.doi.org/10.18844/ijlt.v8i3.895.

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We present a case study of the work with the Methodology of Problem-Solving as an Investigation (MPSI) in the context of the Spanish Master’s in Secondary Education. Here, future physics and chemistry secondary teachers had to solve some sequences of school open-ended problems, in order to promote reflection on its characteristics and favour its later use. The objectives of this research consist of describing how the problem “What might happen when a substance is heated up?” was introduced, and analysing preservice teachers’ achievement levels in the competence dimensions of the MPSI. In addition, the article justifies the suitability of the abovementioned problem to work on most of the curricular requirements about physical and chemical changes in a single problem. The results from the future teachers’ written reports can be considered as positive, and appear to be best for the “formulation of hypotheses” and worst for the “design of resolution strategies”. Moreover, these results do not seem to very much depend on the preservice teachers’ previous studies. Finally, we call for providing more inquiry-based learning opportunities to future teachers, in order to promote improvement in these scientific competencies and favour a later inclusion of the Inquiry-Based Science Education at school levels.

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Ahn, Chiyoung, Mikyoeng Kim, Soojin Lee, Jackwang Kim, Deogsu Hwang, Kyungmoon Park et Jeongbin Yim. « Molecular Analysis of mps Operon Involved in Monapterin Syntliesis in Escherichia Coli ». Pteridines 17, n^o 1 (février 2006) : 16–19. http://dx.doi.org/10.1515/pteridines.2006.17.1.16.

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Abstract Two genes, mpsA and mpsB, constitute an operon in the synthesis of monapterin in E. coli. Expression of mpsA, the structural gene for dihydroneopterin triphosphate 2'-epimerase, from its native promoter, required mpsB. Cotransfonnation of mpsB excludes the possibility that the MpsB is a transactivator protein for mpsA expression.

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Dreyfus, JC. « La maladie de Hurler (mucopolysaccharidose type I MPSI). Mutations et polymorphismes. » médecine/sciences 9, n^o 11 (1993) : 1289. http://dx.doi.org/10.4267/10608/2855.

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Boado, Ruben J. « IgG Fusion Proteins for Brain Delivery of Biologics via Blood–Brain Barrier Receptor-Mediated Transport ». Pharmaceutics 14, n^o 7 (15 juillet 2022) : 1476. http://dx.doi.org/10.3390/pharmaceutics14071476.

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The treatment of neurological disorders with large-molecule biotherapeutics requires that the therapeutic drug be transported across the blood–brain barrier (BBB). However, recombinant biotherapeutics, such as neurotrophins, enzymes, decoy receptors, and monoclonal antibodies (MAb), do not cross the BBB. These biotherapeutics can be re-engineered as brain-penetrating bifunctional IgG fusion proteins. These recombinant proteins comprise two domains, the transport domain and the therapeutic domain, respectively. The transport domain is an MAb that acts as a molecular Trojan horse by targeting a BBB-specific endogenous receptor that induces receptor-mediated transcytosis into the brain, such as the human insulin receptor (HIR) or the transferrin receptor (TfR). The therapeutic domain of the IgG fusion protein exerts its pharmacological effect in the brain once across the BBB. A generation of bifunctional IgG fusion proteins has been engineered using genetically engineered MAbs directed to either the BBB HIR or TfR as the transport domain. These IgG fusion proteins were validated in animal models of lysosomal storage disorders; acute brain conditions, such as stroke; or chronic neurodegeneration, such as Parkinson’s disease and Alzheimer’s disease. Human phase I–III clinical trials were also completed for Hurler MPSI and Hunter MPSII using brain-penetrating IgG-iduronidase and -iduronate-2-sulfatase fusion protein, respectively.

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Fan, Jinbo, Hui Jin, Bailey A. Koch et Hong-Guo Yu. « Mps2 links Csm4 and Mps3 to form a telomere-associated LINC complex in budding yeast ». Life Science Alliance 3, n^o 12 (23 septembre 2020) : e202000824. http://dx.doi.org/10.26508/lsa.202000824.

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The linker of the nucleoskeleton and cytoskeleton (LINC) complex is composed of two transmembrane proteins: the KASH domain protein localized to the outer nuclear membrane and the SUN domain protein to the inner nuclear membrane. In budding yeast, the sole SUN domain protein, Mps3, is thought to pair with either Csm4 or Mps2, two KASH-like proteins, to form two separate LINC complexes. Here, we show that Mps2 mediates the interaction between Csm4 and Mps3 to form a heterotrimeric telomere-associated LINC (t-LINC) complex in budding yeast meiosis. Mps2 binds to Csm4 and Mps3, and all three are localized to the telomere. Telomeric localization of Csm4 depends on both Mps2 and Mps3; in contrast, Mps2’s localization depends on Mps3 but not Csm4. Mps2-mediated t-LINC complex regulates telomere movement and meiotic recombination. By ectopically expressing CSM4 in vegetative yeast cells, we reconstitute the heterotrimeric t-LINC complex and demonstrate its ability to tether telomeres. Our findings therefore reveal the heterotrimeric composition of the t-LINC complex in budding yeast and have implications for understanding variant LINC complex formation.

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Ishak, Ruhaizad, Azah Mohamed, Ahmed N. Abdalla et Mohd Zamri Che Wanik. « Optimal placement and sizing of distributed generators based on a novel MPSI index ». International Journal of Electrical Power & ; Energy Systems 60 (septembre 2014) : 389–98. http://dx.doi.org/10.1016/j.ijepes.2014.03.044.

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Jaspersen, Sue L., Adriana E. Martin, Galina Glazko, Thomas H. Giddings, Garry Morgan, Arcady Mushegian et Mark Winey. « The Sad1-UNC-84 homology domain in Mps3 interacts with Mps2 to connect the spindle pole body with the nuclear envelope ». Journal of Cell Biology 174, n^o 5 (21 août 2006) : 665–75. http://dx.doi.org/10.1083/jcb.200601062.

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The spindle pole body (SPB) is the sole site of microtubule nucleation in Saccharomyces cerevisiae; yet, details of its assembly are poorly understood. Integral membrane proteins including Mps2 anchor the soluble core SPB in the nuclear envelope. Adjacent to the core SPB is a membrane-associated SPB substructure known as the half-bridge, where SPB duplication and microtubule nucleation during G1 occurs. We found that the half-bridge component Mps3 is the budding yeast member of the SUN protein family (Sad1-UNC-84 homology) and provide evidence that it interacts with the Mps2 C terminus to tether the half-bridge to the core SPB. Mutants in the Mps3 SUN domain or Mps2 C terminus have SPB duplication and karyogamy defects that are consistent with the aberrant half-bridge structures we observe cytologically. The interaction between the Mps3 SUN domain and Mps2 C terminus is the first biochemical link known to connect the half-bridge with the core SPB. Association with Mps3 also defines a novel function for Mps2 during SPB duplication.

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Zaldivar, Rafael J., Gerald S. Rellick et J. M. Yang. « Fiber strength utilization in carbon/carbon composites ». Journal of Materials Research 8, n^o 3 (mars 1993) : 501–11. http://dx.doi.org/10.1557/jmr.1993.0501.

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The utilization of tensile strength of carbon fibers in unidirectional carbon/carbon (C/C) composites was studied for a series of four mesophase-pitch-based carbon fibers in a carbon matrix derived from a polyarylacetylene (PAA) resin. The fibers had moduli of 35, 75, 105, and 130 Mpsi. Composite processing conditions ranged from the cured-resin state to various heat-treatment temperatures (HTT's) from 1100 to 2750 °C for the C/C's. Room-temperature tensile strength and modulus were measured for the various processing conditions, and were correlated with SEM observations of fracture surfaces, fiber and matrix microstructures, and fiber/matrix interphase structures. Fiber tensile strength utilization (FSU) is defined as the ratio of apparent fiber strength in the C/C to the fiber strength in an epoxy-resin-matrix composite. Carbonization heat treatment to 1100 °C results in a brittle carbon matrix that bonds strongly with the three lower modulus fibers, resulting in matrix-dominated failure at FSU values of 24 to 35%. However, the composite with the 130-Mpsi-modulus filament had an FSU of 79%. It is attributed to a combination of tough fracture within the filament itself and a weaker fiber/matrix interface. Both factors lead to crack deflection and blunting rather than to crack propagation. The presence of a weakened interface is inferred from observations of fiber pullout. Much of the FSU of the three lower modulus fibers is recovered by HTT to 2100 or 2400 °C, principally as a result of interface weakening, which works to prevent matrix-dominated fracture. With HTT to 2750 °C, there is a drop in FSU for all the composites; it is apparently the result of a combination of fiber degradation and reduced matrix stress-transfer capability.

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Yu, Seok-Ho, Laura Pollard, Tim Wood, Heather Flanagan-Steet et Richard Steet. « A Biochemical Platform to Define the Relative Specific Activity of IDUA Variants Identified by Newborn Screening ». International Journal of Neonatal Screening 6, n^o 4 (12 novembre 2020) : 88. http://dx.doi.org/10.3390/ijns6040088.

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The lysosomal storage disorder, mucopolysaccharidosis I (MPSI), results from mutations in IDUA, the gene that encodes the glycosaminoglycan-degrading enzyme α-L-iduronidase. Newborn screening efforts for MPSI have greatly increased the number of novel IDUA variants identified, but with insufficient experimental evidence regarding their pathogenicity, many of these variants remain classified as variants of uncertain significance (VUS). Defining pathogenicity for novel IDUA variants is critical for decisions regarding medical management and early intervention. Here, we describe a biochemical platform for the characterization of IDUA variants that relies on viral delivery of IDUA DNA into IDUA-deficient HAP1 cells and isolation of single cell expression clones. The relative specific activity of wild-type and variant α-iduronidase was determined using a combination of Western blot analysis and α-iduronidase activity assays. The specific activity of each variant enzyme was consistent across different single cell clones despite variable IDUA expression and could be accurately determined down to 0.05–0.01% of WT α-iduronidase activity. With this strategy we compared the specific activities of known pseudodeficiency variants (p.His82Gln, p.Ala79Thr, p.Val322Glu, p.Asp223Asn) or pathogenic variants (p.Ser633Leu, p.His240Arg) with variants of uncertain significance (p.Ser586Phe, p.Ile272Leu). The p.Ser633Leu and p.His240Arg variants both show very low activities consistent with their association with Scheie syndrome. In our experiments, however, p.His240Arg exhibited a specific activity five times higher than p.Ser633Leu in contrast to other reports showing equivalent activity. Cell clones expressing the p.Ser586Phe and p.Ile272Leu variants had specific activities in the range of other pseudodeficiency variants tested. Our findings show that pseudodeficiency and pathogenic variants can be distinguished from each other with regard to specific activity, and confirms that all the pseudodeficiency variants variably reduce α-iduronidase activity. We envision this platform will be a valuable resource for the rigorous assessment of the novel IDUA variants emerging from the expansion of newborn screening efforts.

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Khimji, I., C. Lepage, L. Zalesky, P. Jimenez, D. Barbouth, L. Elsas, I. Delgado, B. Briery, P. Gordon et G. I. Kleiner. « Combined home enzyme replacement therapy and unrelated cord blood transplant for Hurler’s syndrome (MPSI) ». Biology of Blood and Marrow Transplantation 12, n^o 2 (février 2006) : 129. http://dx.doi.org/10.1016/j.bbmt.2005.11.395.

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Wu, Gang, Long Chen, Chun Ling Deng et Kun Wei. « Fabrication of Ofloxacin/PLGA Microsphere for Bone Tuberculosis Therapy ». Advanced Materials Research 647 (janvier 2013) : 176–80. http://dx.doi.org/10.4028/www.scientific.net/amr.647.176.

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The purpose of this research was to use mesoporous silicon (mpSi) as internal phase additive to improve the hydrophilic ofloxacin loaded by the hydrophobic PLGA materials through a double emulsion (water-in-oil-in-water) solvent extraction/evaporation method. Laser distribution analysis displayed low impact of MS additive on the final particles size. When compared to particle loading efficiency of none internal phase additives, MS internal phase group showed higher loading efficiency, and it increased with MS amounts inside the microparticles. All the burst releases of MS internal phase groups were severe than none MS group and was directly related the MS amount inside the microsphere. The release rate was increasing with the MS amounts added into the internal phase.

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Messina, C., A. Rampazzo, S. Cesaro, C. Monciotti, N. Gasparotto, R. Tomanin et M. Scarpa. « Eighteen-year follow-up of the first Italian MPSI patient treated with bone marrow transplantation ». Bone Marrow Transplantation 41, n^o 10 (18 février 2008) : 905–6. http://dx.doi.org/10.1038/sj.bmt.1705988.

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Alvelo, Jaime, Andres A. Collazo et David Rosario. « Comprehensive Assessment Tools for Hispanics : Validation of the Multi-Problem Screening Inventory (MPSI) for Puerto Ricans ». Research on Social Work Practice 11, n^o 6 (novembre 2001) : 699–724. http://dx.doi.org/10.1177/104973150101100604.

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Kaci, Anna, Emilie Adiceam, Melanie Dupont, Marine Garrido, Jeannig Berrou, Hanane Djamai, Andre Baruchel et al. « Preclinical Activity of the MPS1 Inhibitor S81694 in Acute Lymphoblastic Leukemia (ALL) ». Blood 134, Supplement_1 (13 novembre 2019) : 2631. http://dx.doi.org/10.1182/blood-2019-125641.

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Introduction: The dual-specificity protein kinase, monopolar spindle 1 (Mps1) is one the main kinases of the spindle assembly checkpoint (SAC) critical for accurate segregation of sister chromatids during mitosis. A hallmark of cancer cells is chromosomal instability caused by deregulated cell cycle checkpoints and SAC dysfunction. Mps1 is known to be overexpressed in several solid tumors including triple negative breast cancer. Thus, Mps1 seems to be a promising target and small molecules targeting Mps1 entered clinical trials in solid tumors. ALL originates from malignant transformation of B-and T-lineage lymphoid precursors with a variety of genetic aberrations including chromosome translocations, mutations, and aneuploidies in genes responsible for cell cycle regulation and lymphoid cell development. While outcome is excellent for pediatric patients and younger adults, relapsed and refractory disease still remain a clinical challenge for elder patients. Here, we demonstrate for the first time preclinical efficacy of the small molecule Mps1 inhibitor (Mps1i) S81694 in T- and B- ALL cells including BCR-ABL1+-driven B-ALL. Materials and Methods: Expression of Mps1 was determined by RT-qPCR and WB in JURKAT, RS4-11 and BCR-ABL1+ cells (BV-173 and TOM-1). A small molecule Mps1i (S81694) was tested alone (0 to 1000nM) or in combination with imatinib, dasatinib, nilotinib and ponatinib in BCR-ABL1+ ALL cell lines. Cell viability and IC50 was assessed by MTS assays after exposure to Mps1i for 72h. In combination experiments, compounds were added simultaneously and relative cell numbers were determined at 72h with MTS assays and combination index (CI) values were calculated according to the Bliss model. Induction of apoptosis was evaluated by annexin-V exposure and PI incorporation at 72h with increasing doses of Mps1i. Cell-cycle distribution was determined by cytofluorometric analysis detecting nuclear propidium iodide (PI) intercalation at 48h. Phosphorylation of Mps1 was detected in synchronized (by nocodazole and MG-132) cells by immunofluorescence using an anti phospho-Mps1 antibody detecting Thr33/Ser37 residues. Time-lapse microscopy was used in cell lines in presence or absence of S81694 to determine mitosis duration. Bone marrow (BM) nucleated patient cells were obtained after informed consent and incubated in methylcellulose with cytokines with or without Mps1i for 2 weeks to determine colony growth. Results: Expression of Mps1 could be detected by RT-qPCR and at the protein level by WB in all cell lines (Figure 1A and B ). IC50 after Mps1i exposure alone was 126nM in JURKAT cells, 51nM in RS4-11 cells, 75nM in BV-173 cells and 83nM in TOM-1. Significant apoptosis as detected by phosphatidylserine exposure and PI incorporation in all cell lines with BCR-ABL1+ cell lines BV-173 and TOM-1 cells being the most sensitive (80% and 60% apoptotic cells respectively)(Figure 1C). Upon Mps1i exposure we observed targeted inhibition of Mps1 phosphorylation at Thr33/Ser37 residues indicating the specific on target effect of S81694 by inhibiting Mps1 autophosphorylation (Figure 1D and E). Cell cycle profile was generally lost after treatment with S81694 in all cell lines indicating aberrant 2n/4n distribution due to SAC abrogation (Figure 1F). Furthermore, we demonstrated that S81694 exposure accelerated significantly mitosis in BV-173 cell line from 36 minutes to 19 minutes indicating effective inhibition of SAC function (Figure 1G). Interestingly, S81694 induced significant apoptosis (70%) in the imatinib resistant BV173 cell line bearing the E255K-BCR-ABL1-mutation. Combination of S81694 with TKI imatinib, dasatinib and nilotinib (but not ponatinib) was strongly synergistic in BCR-ABL1+ cells (Figure 1H). Finally, we observed inhibition of colony formation in a patient with BCR-ABL1+ B-ALL after exposure to 100nM and 250nM S81694 (reduction of 85% and 100% respectively)(Figure 1I). Conclusion: Mps1i S81694 yields significant preclinical activity in T-and B-cell ALL including BCR-ABL1+ models. Interestingly S81694 was efficacious in a TKI resistant cell line. Disclosures Kaci: Institut de Recherches Internationales Servier (IRIS): Employment. Garrido:Institut de Recherches Internationales Servier (IRIS): Employment. Burbridge:Institut de Recherches Internationales Servier (IRIS): Employment. Dombret:AGIOS: Honoraria; CELGENE: Consultancy, Honoraria; Institut de Recherches Internationales Servier (IRIS): Research Funding. Braun:Institut de Recherches Internationales Servier (IRIS): Research Funding.

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Winey, M., L. Goetsch, P. Baum et B. Byers. « MPS1 and MPS2 : novel yeast genes defining distinct steps of spindle pole body duplication. » Journal of Cell Biology 114, n^o 4 (15 août 1991) : 745–54. http://dx.doi.org/10.1083/jcb.114.4.745.

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It is crucial to the eucaryotic cell cycle that the centrosome undergo precise duplication to generate the two poles of the mitotic spindle. In the budding yeast Saccharomyces cerevisiae, centrosomal functions are provided by the spindle pole body (SPB), which is duplicated at the time of bud emergence in G1 of the cell cycle. Genetic control of this process has previously been revealed by the characterization of mutants in CDC31 and KAR1, which prevent SPB duplication and lead to formation of a monopolar spindle. Newly isolated mutations described here (mps1 and mps2, for monopolar spindle) similarly cause monopolar mitosis but their underlying effects on SPB duplication are unique. The MPS1 gene is found by electron microscopy to be essential for proper formation of the site at which the new SPB normally arises adjacent to the existing one. By contrast, a mutation in MPS2 permits duplication to proceed, but the newly formed SPB is structurally defective and unable to serve as a functional spindle pole. Distinct temporal requirements for the CDC31, MPS1, and MPS2 gene functions during the SPB duplication cycle further demonstrate the individual roles of these genes in the morphogenetic pathway.

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Wang, Shao Hui, Zhong Yi Cai, Ming Zhe Li et Ying Wu Lan. « Numerical Analysis of the Multi-Point Stretch Forming Process of Aircraft Outer Skin Part ». Advanced Materials Research 154-155 (octobre 2010) : 1068–72. http://dx.doi.org/10.4028/www.scientific.net/amr.154-155.1068.

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As a flexible manufacturing technique, Multi-point stretch forming (MPSF) is a suitable method for forming aircraft outer skin part. The traditional solid stretching die is replaced by the discrete multi-point die (MPSD), and the sheet metal is stretch-formed over the MPSD generated by serial adjusting mode or parallel adjusting mode. The MPSF can be used to form the parts of different shape and reduce the cost and leading time of stretching die fabrication for aircraft outer skin part. A series of numerical simulations on typical MPSF processes of aircraft outer skin part were carried out. The thickness of elastic cushion and free length are important factors to influence on the stretch forming results of stretch-formed parts. The numerical simulation results show that the thicker the elastic cushion is, the more valid the dimple will be suppressed .The longer the free length is, the easier the wrinkle will be brought.

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Araki, Yasuhiro, Linda Gombos, Suellen P. S. Migueleti, Lavanya Sivashanmugam, Claude Antony et Elmar Schiebel. « N-terminal regions of Mps1 kinase determine functional bifurcation ». Journal of Cell Biology 189, n^o 1 (5 avril 2010) : 41–56. http://dx.doi.org/10.1083/jcb.200910027.

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Mps1 is a conserved kinase that in budding yeast functions in duplication of the spindle pole body (SPB), spindle checkpoint activation, and kinetochore biorientation. The identity of Mps1 targets and the subdomains that convey specificity remain largely unexplored. Using a novel combination of systematic deletion analysis and chemical biology, we identified two regions within the N terminus of Mps1 that are essential for either SPB duplication or kinetochore biorientation. Suppression analysis of the MPS1 mutants defective in SPB duplication and biochemical enrichment of Mps1 identified the essential SPB components Spc29 and the yeast centrin Cdc31 as Mps1 targets in SPB duplication. Our data suggest that phosphorylation of Spc29 by Mps1 in G1/S recruits the Mps2–Bbp1 complex to the newly formed SPB to facilitate its insertion into the nuclear envelope. Mps1 phosphorylation of Cdc31 at the conserved T110 residue controls substrate binding to Kar1 protein. These findings explain the multiple SPB duplication defects of mps1 mutants on a molecular level.

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King, Kelly, Kyle Rudser, Victor Kovac, Igor Nestrasil, Kathleen Delaney et Elsa Shapiro. « Visual memory and working memory as assessed by a computerized measure in individuals with mucopolysaccharidosis, Type I (MPSI) ». Molecular Genetics and Metabolism 108, n^o 2 (février 2013) : S53. http://dx.doi.org/10.1016/j.ymgme.2012.11.128.

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Gomez, Angelo, Sergio Gonzales et Juan C. Francia-Quiroz. « Efecto de la temperatura y concentración de microplásticos en la tasa de filtración del mejillón Semimytilus algosus (Mytiloida : Mytilidae) ». Revista de Biología Tropical 69, n^o 4 (17 novembre 2021) : 1242–51. http://dx.doi.org/10.15517/rbt.v69i4.45426.

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Introducción: La presencia de microplásticos (MPs, partículas menores a 5 mm) y el incremento de la temperatura en los océanos, vienen generando perturbaciones en la vida marina, que se pueden relacionar con alteraciones en el metabolismo de organismos filtradores, como los mitílidos. Objetivo: Se evalúa el efecto de diferentes temperaturas y concentraciones de MPs sobre la tasa de filtración (TF) de Semimytilus algosus. Métodos: Una muestra de organismos (N = 72) fue expuesta a cuatro temperaturas (17, 20, 23 y 26 °C), y un testigo sin microplásticos (MPs0) y dos concentraciones de MPs (< 125 µm) de 0.125 mg/l (MPs1) y 0.250 mg/l (MPs2), todos en combinación con la microalga Isochrysis galbana (1x106 cel/ml/día) por 21 días. Resultados: A medida que aumentó la concentración de MPs, se redujo la TF de S. algosus. Respecto a la temperatura, durante el día 7 se observó una mayor TF a 23 °C en todos los tratamientos, y para los días 14 y 21 se obtuvieron los menores valores de TF a 23 y 26 °C. La acción conjunta del incremento de temperatura y MPs, afectó negativamente la TF de S. algosus, donde ambos factores ocasionaron el descenso de la TF para todos los tiempos de evaluación. No se registró mortalidad a 17 °C para ningún tratamiento, y en el caso de mitílidos expuestos a MPs1 y temperaturas de 20 y 26 °C se presentó la mayor mortalidad (67 %). Conclusiones: El estudio demuestra el efecto adverso del incremento de temperatura y MPs sobre la TF de S. algosus.

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da Silva, Flávia, Vanessa Pereira, Eduardo G. Yasumura, Lígia Tenório, Leonardo de Carvalho, Bianca Cristina Lisboa, Priscila Matsumoto et al. « Treatment of adult MPSI mouse brains with IDUA-expressing mesenchymal stem cells decreases GAG deposition and improves exploratory behavior ». Genetic Vaccines and Therapy 10, n^o 1 (2012) : 2. http://dx.doi.org/10.1186/1479-0556-10-2.

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Rodriguez, M., I. Khimji, S. Rhodd, C. Lapage, L. Zalesky, P. Jimenez, D. Barbouth, L. Elsas et G. Kleiner. « Combined home enzyme replacement therapy and unrelated cord blood transplantation for Hurler’s syndrome (MPSI) in a pediatric HSCT center ». Biology of Blood and Marrow Transplantation 11, n^o 2 (février 2005) : 97. http://dx.doi.org/10.1016/j.bbmt.2004.12.286.

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Gao, Mingxiao, Xinyuan Zhang, Yangwu Guo, Jiyong Yao, Guochun Zhang, Zhanggui Hu et Yicheng Wu. « Rational structure design of isoxazolone-based crystals with large second-order optical nonlinearity ». CrystEngComm 22, n^o 39 (2020) : 6444–47. http://dx.doi.org/10.1039/d0ce01100b.

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Two new acentric isoxazolone-based organic crystals of C₂₄H₁₇NO₃ (MPFI) and C₁₈H₁₅NO₃S (MPMI) were designed and synthesized by a structure fine-tuning strategy.

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Jeong, Hyun, Kwon Lee, Min Kim, Sung Kim, Min Kim et Ho Kim. « Signal Intensity of Contrast Enhancement according to TE in 3.0T MRI T1 Imaging ». Applied Sciences 8, n^o 7 (13 juillet 2018) : 1138. http://dx.doi.org/10.3390/app8071138.

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Normal body tissue or lesion characteristics in T1 images have been evaluated; however, how external parameters effect the change in signal intensity by gadolinium-based contrast agent remains unknown. We investigated how contrast enhancement changed according to echo time (TE) in 3.0T magnetic resonance (MR) T1 imaging and determined the optimal settings for TE in contrast-enhanced T1 imaging. Since there are no guidelines regarding parameters for T1 enhancement when using MR-contrast agents, we analyzed results from varying TEs (between 25 and 7 msec) in both a phantom and clinical study. We obtained the following results: contrast percentage of fat to saline increased from 740.0–1003.6%, response start point increased from 30–90 mmol, max peak signal intensity increased from 1771–2425 a.u., max peak point increased from 2–4 mmol, enhancement percentage of the max peak signal intensity (MPSI) to saline increased from 1671.0–2065.2%, the average of SI on each mol as TE increased from 600.8–996.6 a.u., the average of SI as TE on each molar concentration increased from 378–845 a.u., the AEPSS increased from 44.3–140.3%, and the AEPSC increased from 224.3–647.8%. We confirmed that TE can affect contrast enhancement, and the lowest TE has faster and higher effects on contrast enhancement.

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Alvelo Burgos, Jaime, et Luz Enid Del Valle. « La adaptación y validación al español de una batería de 27 escalas (MPSI) para medir problemas psicosociales con una muestra puertorriqueña ». AnálisiS 4, n^o 1 (21 mars 2003) : 225–47. http://dx.doi.org/10.54114/revanlisis.v4i1.13423.

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Weiss, E., et M. Winey. « The Saccharomyces cerevisiae spindle pole body duplication gene MPS1 is part of a mitotic checkpoint. » Journal of Cell Biology 132, n^o 1 (1 janvier 1996) : 111–23. http://dx.doi.org/10.1083/jcb.132.1.111.

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M-phase checkpoints inhibit cell division when mitotic spindle function is perturbed. Here we show that the Saccharomyces cerevisiae MPS1 gene product, an essential protein kinase required for spindle pole body (SPB) duplication (Winey et al., 1991; Lauze et al., 1995), is also required for M-phase check-point function. In cdc31-2 and mps2-1 mutants, conditional failure of SPB duplication results in cell cycle arrest with high p34CDC28 kinase activity that depends on the presence of the wild-type MAD1 checkpoint gene, consistent with checkpoint arrest of mitosis. In contrast, mps1 mutant cells fail to duplicate their SPBs and do not arrest division at 37 degrees C, exhibiting a normal cycle of p34CDC28 kinase activity despite the presence of a monopolar spindle. Double mutant cdc31-2, mps1-1 cells also fail to arrest mitosis at 37 degrees C, despite having SPB structures similar to cdc31-2 single mutants as determined by EM analysis. Arrest of mitosis upon microtubule depolymerization by nocodazole is also conditionally absent in mps1 strains. This is observed in mps1 cells synchronized in S phase with hydroxyurea before exposure to nocodazole, indicating that failure of checkpoint function in mps1 cells is independent of SPB duplication failure. In contrast, hydroxyurea arrest and a number of other cdc mutant arrest phenotypes are unaffected by mps1 alleles. We propose that the essential MPS1 protein kinase functions both in SPB duplication and in a mitotic checkpoint monitoring spindle integrity.

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Yi, Houqin, Ganjun Yuan, Shimin Li, Xuejie Xu, Yingying Guan, Li Zhang et Yu Yan. « Drug Combinations to Prevent Antimicrobial Resistance : Various Correlations and Laws, and Their Verifications, Thus Proposing Some Principles and a Preliminary Scheme ». Antibiotics 11, n^o 10 (20 septembre 2022) : 1279. http://dx.doi.org/10.3390/antibiotics11101279.

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Antimicrobial resistance (AMR) has been a serious threat to human health, and combination therapy is proved to be an economic and effective strategy for fighting the resistance. However, the abuse of drug combinations conversely accelerates the spread of AMR. In our previous work, we concluded that the mutant selection indexes (SIs) of one agent against a specific bacterial strain are closely related to the proportions of two agents in a drug combination. To discover probable correlations, predictors and laws for further proposing feasible principles and schemes guiding the AMR-preventing practice, here, three aspects were further explored. First, the power function (y = axb, a > 0) correlation between the SI (y) of one agent and the ratio (x) of two agents in a drug combination was further established based on the mathematical and statistical analyses for those experimental data, and two rules a1 × MIC1 = a2 × MIC2 and b1 + b2 = −1 were discovered from both equations of y = a1xb1 and y = a2xb2 respectively for two agents in drug combinations. Simultaneously, it was found that one agent with larger MPC alone for drug combinations showed greater potency for narrowing itself MSW and preventing the resistance. Second, a new concept, mutation-preventing selection index (MPSI) was proposed and used for evaluating the mutation-preventing potency difference of two agents in drug combination; a positive correlation between the MPSI and the mutant prevention concentration (MPC) or minimal inhibitory concentration (MIC) was subsequently established. Inspired by this, the significantly positive correlation, contrary to previous reports, between the MIC and the corresponding MPC of antimicrobial agents against pathogenic bacteria was established using 181 data pairs reported. These results together for the above three aspects indicate that the MPCs in alone and combination are very important indexes for drug combinations to predict the mutation-preventing effects and the trajectories of collateral sensitivity, and while the MPC of an agent can be roughly calculated from its corresponding MIC. Subsequently, the former conclusion was further verified and improved via antibiotic exposure to 43 groups designed as different drug concentrations and various proportions. The results further proposed that the C/MPC for the agent with larger proportion in drug combinations can be considered as a predictor and is the key to judge whether the resistance and the collateral sensitivity occur to two agents. Based on these above correlations, laws, and their verification experiments, some principles were proposed, and a diagram of the mutation-preventing effects and the resistant trajectories for drug combinations with different concentrations and ratios of two agents was presented. Simultaneously, the reciprocal of MPC alone (1/MPC), proposed as the stress factors of two agents in drug combinations, together with their SI in combination, is the key to predict the mutation-preventing potency and control the trajectories of collateral sensitivity. Finally, a preliminary scheme for antimicrobial combinations preventing AMR was further proposed for subsequent improvement research and clinic popularization, based on the above analyses and discussion. Moreover, some similar conclusions were speculated for triple or multiple drug combinations.

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Chen, Jingjing, Jennifer M. Gardner, Zulin Yu, Sarah E. Smith, Sean McKinney, Brian D. Slaughter, Jay R. Unruh et Sue L. Jaspersen. « Yeast centrosome components form a noncanonical LINC complex at the nuclear envelope insertion site ». Journal of Cell Biology 218, n^o 5 (12 mars 2019) : 1478–90. http://dx.doi.org/10.1083/jcb.201809045.

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Bipolar spindle formation in yeast requires insertion of centrosomes (known as spindle pole bodies [SPBs]) into fenestrated regions of the nuclear envelope (NE). Using structured illumination microscopy and bimolecular fluorescence complementation, we map protein distribution at SPB fenestrae and interrogate protein–protein interactions with high spatial resolution. We find that the Sad1-UNC-84 (SUN) protein Mps3 forms a ring-like structure around the SPB, similar to toroids seen for components of the SPB insertion network (SPIN). Mps3 and the SPIN component Mps2 (a Klarsicht-ANC-1-Syne-1 domain [KASH]–like protein) form a novel noncanonical linker of nucleoskeleton and cytoskeleton (LINC) complex that is connected in both luminal and extraluminal domains at the site of SPB insertion. The LINC complex also controls the distribution of a soluble SPIN component Bbp1. Taken together, our work shows that Mps3 is a fifth SPIN component and suggests both direct and indirect roles for the LINC complex in NE remodeling.

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La Cognata, Valentina, Maria Guarnaccia, Giovanna Morello, Martino Ruggieri, Agata Polizzi et Sebastiano Cavallaro. « Design and Validation of a Custom NGS Panel Targeting a Set of Lysosomal Storage Diseases Candidate for NBS Applications ». International Journal of Molecular Sciences 22, n^o 18 (17 septembre 2021) : 10064. http://dx.doi.org/10.3390/ijms221810064.

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Lysosomal storage diseases (LSDs) are a heterogeneous group of approximately 70 monogenic metabolic disorders whose diagnosis represents an arduous challenge for clinicians due to their variability in phenotype penetrance, clinical manifestations, and high allelic heterogeneity. In recent years, the approval of disease-specific therapies and the rapid emergence of novel rapid diagnostic methods has opened, for a set of selected LSDs, the possibility for inclusion in extensive national newborn screening (NBS) programs. Herein, we evaluated the clinical utility and diagnostic validity of a targeted next-generation sequencing (tNGS) panel (called NBS_LSDs), designed ad hoc to scan the coding regions of six genes (GBA, GAA, SMPD1, IDUA1, GLA, GALC) relevant for a group of LSDs candidate for inclusion in national NBS programs (MPSI, Pompe, Fabry, Krabbe, Niemann Pick A-B and Gaucher diseases). A standard group of 15 samples with previously known genetic mutations was used to test and validate the entire flowchart. Analytical accuracy, sensitivity, and specificity, as well as turnaround time and costs, were assessed. Results showed that the Ion AmpliSeq and Ion Chef System-based high-throughput NBS_LSDs tNGS panel is a fast, accurate, and cost-effective process. The introduction of this technology into routine NBS procedures as a second-tier test along with primary biochemical assays will allow facilitating the identification and management of selected LSDs and reducing diagnostic delay.

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Schutz, Amy R., Thomas H. Giddings, Estelle Steiner et Mark Winey. « The Yeast CDC37 Gene Interacts with MPS1 and Is Required for Proper Execution of Spindle Pole Body Duplication ». Journal of Cell Biology 136, n^o 5 (10 mars 1997) : 969–82. http://dx.doi.org/10.1083/jcb.136.5.969.

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The MPS1 gene from Saccharomyces cerevisiae encodes an essential protein kinase required for spindle pole body (SPB) duplication and for the mitotic spindle assembly checkpoint. Cells with the mps1-1 mutation fail early in SPB duplication and proceed through monopolar mitosis with lethal consequences. We identified CDC37 as a multicopy suppressor of mps1-1 temperature-sensitive growth. Suppression is allele specific, and synthetic lethal interactions occur between mps1 and cdc37 alleles. We examined the cdc37-1 phenotype for defects related to the SPB cycle. The cdc37-1 temperature-sensitive allele causes unbudded, G1 arrest at Start (Reed, S.I. 1980. Genetics. 95: 561–577). Reciprocal shifts demonstrate that cdc37-1 arrest is interdependent with α-factor arrest but is not a normal Start arrest. Although the cells are responsive to α-factor at the arrest, SPB duplication is uncoupled from other aspects of G1 progression and proceeds past the satellite-bearing SPB stage normally seen at Start. Electron microscopy reveals side-by-side SPBs at cdc37-1 arrest. The outer plaque of one SPB is missing or reduced, while the other is normal. Using the mps2-1 mutation to distinguish between the SPBs, we find that the outer plaque defect is specific to the new SPB. This phenotype may arise in part from reduced Mps1p function: although Mps1p protein levels are unaffected by the cdc37-1 mutation, kinase activity is markedly reduced. These data demonstrate a requirement for CDC37 in SPB duplication and suggest a role for this gene in G1 control. CDC37 may provide a chaperone function that promotes the activity of protein kinases.

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Ismail, Fadillah, Zuhaimy Ismail, Mohd Azhar Abd Hamid, Ismail Mohamad, Adibah Abdul Kadir et Kassim Thukiman. « Malaysian People Financial Prosperity ». International Journal of Engineering & ; Technology 7, n^o 2.29 (22 mai 2018) : 1063. http://dx.doi.org/10.14419/ijet.v7i2.29.14311.

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During the first sitting of the fifth term of the 13th Malaysian Parliament, The Yang di-Pertuan Agong Sultan Muhammad V pledged to reign in a just and fair manner by placing the well-being of the people and prosperity of the nation above all else. Thus it becomes the task of the Malaysian Government which includes the financial prosperity of the people. This paper aims at identifying the level of financial prosperity of Malaysian people. It has been identified that financial prosperity is a major problems among Malaysians, a result obtained from a survey research with 2000 samples taken from the whole country which is in accordance with the standards set by the International Statistical Institute [ISI]. The data collected were analyzed using various statistical methods and one of the method used to determine the people financial prosperity or financial well-being is by using index, namely the Malaysian People Satisfaction Index [MPSI]. We categorized the financial prosperity into four levels, namely the poor income, moderate income, good income and excellent income. The survey results show that the level of financial prosperity for Malaysian people is 49.6% with a mean of 4.88 which is categorized as moderate income. Further analysis shows that there are significant differences between the respondents' demographic factors and the financial prosperity. This shows that the government and relevant agencies need to play a more significant role to ensure the policies that are enacted can contribute to the well-being of the people. It would just be very difficult for Malaysia to become a developed nation by 2020 if the status of financial prosperity or financial well-being of its people is still at moderate level income.

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Cheng, Wei-Chieh, Cheng-Kun Lin, Huang-Yi Li, Yu-Chien Chang, Sheng-Jhih Lu, Yu-Shin Chen et Shih-Ying Chang. « A combinatorial approach towards the synthesis of non-hydrolysable triazole–iduronic acid hybrid inhibitors of human α-l-iduronidase : discovery of enzyme stabilizers for the potential treatment of MPSI ». Chemical Communications 54, n^o 21 (2018) : 2647–50. http://dx.doi.org/10.1039/c7cc09642a.

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Gracco, Antonio, Giovanni Bruno, Alberto De Stefani, Laura Siviero, Alessandro Perri et Edoardo Stellini. « Maturation of the Middle Phalanx of the Third Finger : A Comparative Study between Right and Left Hand ». Journal of Clinical Pediatric Dentistry 42, n^o 2 (1 janvier 2018) : 161–65. http://dx.doi.org/10.17796/1053-4628-42.2.14.

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Objectives: Recently a classification of patient's skeletal age based on the phalanx maturation, The Middle Phalanx Maturation of the third finger (MPM) method, was suggested. The aim of this study is to evaluate if there is a difference in MPM between the right and left hand. Study design: Two hundred fifty-four patients were obtained from the Complex Operating Unit of Orthodontics of Padua University Hospital. The total sample size has been selected by appropriate statistical calculations resulting in 130 patients. It was decided to further double the sample size of a previous study to ensure a robust statistical analysis. Radiographs of the right and left were obtained using the MPM method. Stages were compared using the right hand as a reference. The statistical analysis (Fisher exact test) was performed for the entire sample and related to gender in order to compare the right and the left hand stages. Results: In MPS2, 6 out 49 (12.2%) males and 7 out 27 females (25.9%) showed MPS3 in the left hand (p-value < 0.05). In all other stages, a total agreement (100%) was found. Conclusion: The authors confirm the use of the right hand as reference. In patients with MPS2 an additional radiograph on the left hand can be taken in order to increase the diagnostic accuracy. In all other stages other radiographs are not needed as a total agreement between the right and left hand was found.

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Mohanty, Subhadarshini, Prashanta Kumar Patra, Subasish Mohapatra et Mitrabinda Ray. « MPSO ». International Journal of Applied Evolutionary Computation 8, n^o 1 (janvier 2017) : 1–25. http://dx.doi.org/10.4018/ijaec.2017010101.

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Cloud computing is gaining more popularity due to its advantages over conventional computing. It offers utility based services to subscribers on demand basis. Cloud hosts a variety of web applications and provides services on the pay-per-use basis. As the users are increasing in the cloud system, the load balancing has become a critical issue. Scheduling workloads in the cloud environment among various nodes are essential to achieving a better Quality of Service (QOS). It is a prominent area of research as well as challenging to allocate the resources with changeable capacities and functionality. In this paper, a load balancing algorithm using Multi Particle Swarm Optimization (MPSO) has been developed by utilizing the benefits of particle swarm optimization (PSO) algorithm. Proposed approach aims to minimize the task overhead and maximize the resource utilization in a homogenous cloud environment. Performance comparisons are made with Genetic Algorithm (GA), Multi GA, PSO and other popular algorithms on different measures like makespan calculation and resource utilization.

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Schultz, David, Barbara Liskov et Moses Liskov. « MPSS ». ACM Transactions on Information and System Security 13, n^o 4 (décembre 2010) : 1–32. http://dx.doi.org/10.1145/1880022.1880028.

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Fratazzi, C., R. D. Arbeit, C. Carini et H. G. Remold. « Programmed cell death of Mycobacterium avium serovar 4-infected human macrophages prevents the mycobacteria from spreading and induces mycobacterial growth inhibition by freshly added, uninfected macrophages. » Journal of Immunology 158, n^o 9 (1 mai 1997) : 4320–27. http://dx.doi.org/10.4049/jimmunol.158.9.4320.

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Abstract Mycobacterium avium, an opportunistic pathogen in AIDS patients, replicates in human macrophages (Mphi) and induces programmed cell death (PCD). In this study we examine the effect of freshly added, uninfected Mphi on M. avium growth in apoptotic Mphi cultures. Incubation of uninfected autologous Mphi with apoptotic Mphi infected with M. avium for 6 h results in 90% inhibition of bacterial growth. The uninfected Mphi adhere to M. avium-infected apoptotic, but not to nonapoptotic M. avium-infected Mphi, suggesting a specific interaction between apoptotic and nonapoptotic Mphi. PCD of the host Mphi also prevents the release of intracellular components and the spread of the mycobacterial infection. Once the apoptotic infected Mphi reach the necrotic stage, mycobacteria and other intracellular material are released; the latter suffice to support extracellular mycobacterial replication. Necrosis of M. avium-infected Mphi is significantly augmented by the transglutaminase inhibitors dansyl-cadaverine and cystamine, indicating that apoptosis of Mphi is dependent on the extent of cross-linking of cell proteins by transglutaminases. Consequently, transglutaminase inhibitors accelerate the release of mycobacteria and intracellular components from the infected Mphi into the medium. These findings indicate that PCD of M. avium-infected Mphi is an important defense mechanism, preventing the spread of infection by sequestering the mycobacteria and by contributing to their demise by activation of newly recruited uninfected Mphi.

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Kasbek, Christopher, Ching-Hui Yang et Harold A. Fisk. « Antizyme Restrains Centrosome Amplification by Regulating the Accumulation of Mps1 at Centrosomes ». Molecular Biology of the Cell 21, n^o 22 (15 novembre 2010) : 3878–89. http://dx.doi.org/10.1091/mbc.e10-04-0281.

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Extra centrosomes are found in many tumors, and their appearance is an early event that can generate aberrant mitotic spindles and aneuploidy. Because the failure to appropriately degrade the Mps1 protein kinase correlates with centrosome overproduction in tumor-derived cells, defects in the factors that promote Mps1 degradation may contribute to extra centrosomes in tumors. However, while we have recently characterized an Mps1 degradation signal, the factors that regulate Mps1 centrosomal Mps1 are unknown. Antizyme (OAZ), a mediator of ubiquitin-independent degradation and a suspected tumor suppressor, was recently shown to localize to centrosomes and modulate centrosome overproduction, but the known OAZ substrates were not responsible for its effect on centrosomes. We have found that OAZ exerts its effect on centrosomes via Mps1. OAZ promotes the removal of Mps1 from centrosomes, and centrosome overproduction caused by reducing OAZ activity requires Mps1. OAZ binds to Mps1 via the Mps1 degradation signal and modulates the function of Mps1 in centrosome overproduction. Moreover, OAZ regulates the canonical centrosome duplication cycle, and reveals a function for Mps1 in procentriole assembly. Together, our data suggest that OAZ restrains the assembly of centrioles by controlling the levels of centrosomal Mps1 through the Cdk2-regulated Mps1 degradation signal.

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