Thèses sur le sujet « Molecular gene cloning »
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Seto, Nina Oi Ling. « The copper-zinc superoxide dismutase gene from Drosophila melanogaster : attempts to clone the gene using two mixed sequence oligonucleotide probes ». Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26534.
Texte intégralMedicine, Faculty of
Biochemistry and Molecular Biology, Department of
Graduate
Bates, Nancy Carol. « Characterization of cbg : a cloned gene encoding an extracellular [beta]-glucosidase from Cellulomonas fimi ». Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26163.
Texte intégralScience, Faculty of
Microbiology and Immunology, Department of
Graduate
Moser, Bernhard. « Molecular cloning, characterization and expression of the endoglucanase C gene of Cellulomonas fimi and properties of the native and recombinant gene products ». Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/29036.
Texte intégralScience, Faculty of
Microbiology and Immunology, Department of
Graduate
Woodruff, Wendy Anne. « Cloning and characterization of the oprF gene for protein F from Pseudomonas aeruginosa ». Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/29218.
Texte intégralScience, Faculty of
Microbiology and Immunology, Department of
Graduate
Wakarchuk, Warren William. « The molecular cloning and characterization of a Beta-glucosidase gene from an Agrobacterium ». Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/27559.
Texte intégralScience, Faculty of
Microbiology and Immunology, Department of
Graduate
Zhang, Ling 1962. « Molecular cloning and characterization of the chicken ornithine decarboxylase gene ». Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=22831.
Texte intégralFisher, Simon E. « Positional cloning of the gene responsible for Dent's disease ». Thesis, University of Oxford, 1995. http://ora.ox.ac.uk/objects/uuid:22f6e7a5-4f00-41c9-a1d3-1b05899f22c0.
Texte intégralPauliny, Angela. « Cloning and molecular characterisation of the zebrafish colourless gene ». Thesis, University of Bath, 2002. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393804.
Texte intégralBaker, N. E. « Wingless : A gene required for segmentation in Drosophila ». Thesis, University of Cambridge, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.377244.
Texte intégralOza, Kalpesh. « Cloning of a DNA repair gene (uvsF) from Aspergillus ». Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=59577.
Texte intégralHuo, Longfei, et 霍龍飛. « Molecular cloning and functional studies of cyprinid calmodulin ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B3016316X.
Texte intégralYowe, David Langdon. « Molecular cloning and characterisation of the Barramundi growth hormone gene ». Thesis, Queensland University of Technology, 1994.
Trouver le texte intégralJiang, Qijiao. « Cloning and characterization of midgut-specific gene/gene products in the mosquito Aedes aegypti ». Diss., The University of Arizona, 1996. http://hdl.handle.net/10150/282179.
Texte intégralLiang, Rongti. « Molecular cloning and characterization of the ovine placental lactogen gene / ». free to MU campus, to others for purchase, 1996. http://wwwlib.umi.com/cr/mo/fullcit?p9717169.
Texte intégralDriscoll, Barbara. « Cloning and expression of the bovine papillomavirus major capsid gene ». Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184475.
Texte intégralBingle, Lewis Edward Hector. « The molecular genetics of polyketide biosynthesis in filamentous fungi ». Thesis, University of Bristol, 1997. http://hdl.handle.net/1983/15ebe2f2-ab67-47b5-ae4b-1f00140561a1.
Texte intégral胡可進 et Kejin Hu. « Molecular cloning and characterization of the cathepsin L gene from the marine shrimp metapenaeus ensis ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B3124421X.
Texte intégralSeto, Nina O. L. « Cloning and expression of the Drosophila melanogaster CuZn superoxide dismutase gene ». Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/30971.
Texte intégralArts, Faculty of
Philosophy, Department of
Graduate
Hughes, John Michael Xavier. « Molecular analysis of small RNAs of Saccharomyces cerevisiae ». Thesis, University of Leicester, 1988. http://hdl.handle.net/2381/35256.
Texte intégralMarcantonio, Daniela. « Cloning and characterization of a novel steroid hormone responsive gene ». Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=37773.
Texte intégralFinch, Jennie Louise. « Cloning and characterisation of the human uroplakin 1B gene / ». Title page, contents and summary only, 1998. http://web4.library.adelaide.edu.au/theses/09PH/09phf4918.pdf.
Texte intégralLai, Kun-Nan. « Cloning and expression of cambialistic Bacteroides fragilis superoxide dismutase gene ». Diss., This resource online, 1992. http://scholar.lib.vt.edu/theses/available/etd-05042006-164528/.
Texte intégralFan, Xiaohui. « Uroguanylin : molecular cloning and characterization of a potential natriuretic hormone / ». free to MU campus, to others for purchase, 1997. http://wwwlib.umi.com/cr/mo/fullcit?p9841285.
Texte intégralBoddy, Lynn M. « Regulation and molecular cloning of an invertase gene from Aspergillus niger ». Thesis, University of Nottingham, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336021.
Texte intégralJoseph, Rajiv. « Molecular cloning of neuronatin : a novel brain-specific mammalian developmental gene ». Thesis, University of London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243807.
Texte intégralRaitt, Desmond C. « Molecular cloning and characterisation of the Aspergillus nidulans cytochrome c gene ». Thesis, Cranfield University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305383.
Texte intégralHelps, Nicholas Royston. « Cloning and molecular analysis of an evolutionarily conserved Drosophila melanogaster gene ». Thesis, University of Leicester, 1993. http://hdl.handle.net/2381/35249.
Texte intégralSAEDI, MOHAMMAD SAEED. « CLONING OF BACTERIOPHAGE-PHI29 GENE 15 ; ISOLATION, OVERPRODUCTION AND PURIFICATION OF PHI29 LYTIC ENZYME ». Diss., The University of Arizona, 1987. http://hdl.handle.net/10150/184008.
Texte intégral馬忠華 et Chung-wah Ma. « Genomic isolation and molecular analysis of a rat [alpha]-globin gene cluster ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B31237514.
Texte intégralMa, Chung-wah. « Genomic isolation and molecular analysis of a rat [alpha]-globin gene cluster / ». Hong Kong : University of Hong Kong, 1998. http://sunzi.lib.hku.hk/hkuto/record.jsp?B19473047.
Texte intégralZvejnieks, Peter Andrew. « Cloning of an embryo-specific gene from Daucas Carota cDNA library ». Thesis, Georgia Institute of Technology, 1990. http://hdl.handle.net/1853/25408.
Texte intégralAgarwal, Anika. « Molecular cloning and characterisation of the human oviduct-specific glycoprotein (HuOGP) promoter ». Thesis, Hong Kong : University of Hong Kong, 2002. http://sunzi.lib.hku.hk/hkuto/record.jsp?B25335273.
Texte intégralGrove, Heather Lee. « Cloning and characterization of the Pichia Pastoris PMR1 gene ». Scholarly Commons, 2005. https://scholarlycommons.pacific.edu/uop_etds/613.
Texte intégralSimons, Kristin Jean. « Cloning and characterization of the wheat domestication gene, Q ». Diss., Manhattan, Kan. : Kansas State University, 2005. http://hdl.handle.net/2097/135.
Texte intégralLonie, Andrew. « Cloning and characterisation of the Polycomblike gene, a transacting repressor of homeotic gene expression in Drosophila ». Title page, contents and summary only, 1994. http://hdl.handle.net/2440/21504.
Texte intégral{59} leaves : ill. ; 30 cm.
Title page, contents and abstract only. The complete thesis in print form is available from the University Library.
The Polycomblike gene of Drosophila melanogaster is required for the correct spatial expression of the homeotic genes of Antenapaedia and Bithorax Complexes. This thesis describes the isolation and molecular characterization of the Polycomblike gene.
Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry, 1995
Hidalgo-Downing, Alicia. « Molecular cloning of patched and analysis of its role in intrasegmental patterning in D. melanogaster ». Thesis, University of Oxford, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.258158.
Texte intégralSmith, Simon A. « Molecular analysis of the yeast cell cycle : isolation and characterization of a new gene, TSM3721 ». Thesis, University of Manchester, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.253128.
Texte intégralCluness, Martin James. « Cloning and molecular analysis of the cyanide hydrotase gene of Fusarium lateritium ». Thesis, University of Sunderland, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278926.
Texte intégralKosmidou, Effie. « Molecular cloning and characterisation of the pp2a#alpha# gene in Aspergillus nidulans ». Thesis, University of East Anglia, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361419.
Texte intégralMoffat, K. G. « The molecular cloning of the mutT gene of Escherichia coli K-12 ». Thesis, Cranfield University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373991.
Texte intégralBenson, Fiona Elizabeth. « Molecular cloning and analysis of the ruv gene of Escherichia coli K12 ». Thesis, University of Nottingham, 1988. http://eprints.nottingham.ac.uk/30593/.
Texte intégral« Goldfish (Carassius auratus) somatolactin : gene cloning and gene expression studies ». 1999. http://library.cuhk.edu.hk/record=b5889873.
Texte intégralThesis (M.Phil.)--Chinese University of Hong Kong, 1999.
Includes bibliographical references (leaves 123-133).
Abstracts in English and Chinese.
ACKNOWLEDGMENTS --- p.i
ABSTRACT --- p.ii
槪論 --- p.iii
ABBREVIATIONS --- p.iv
AMINO ACIDS SHORTHAND --- p.vi
TABLE OF CONTENTS --- p.vii-x
Chapter CHAPTER 1 --- LITERATURE REVIEW
Chapter 1.1 --- Introduction --- p.1
Chapter 1.2 --- Structural Analysis of SL --- p.1
Chapter 1.3 --- Location of SL-producing cells and Expression of SL --- p.5
Chapter 1.4 --- Possible Functions of SL --- p.9
Chapter 1.4.1 --- Adaptation to various backgrounds and Intensities of Illuminations --- p.9
Chapter 1.4.2 --- Control of Reproduction and Maturation --- p.10
Chapter 1.4.3 --- Responses to Stress --- p.12
Chapter 1.4.4 --- Regulation of P034- and Ca2+ Metabolism --- p.12
Chapter 1.4.5 --- Acid - Base Balance --- p.14
Chapter 1.4.6 --- Regulation of Energy Metabolism --- p.15
Chapter 1.4.7 --- Regulation of Fat Metabolism --- p.15
Chapter 1.5 --- Regulation of SL Gene Expression --- p.19
Chapter 1.5.1 --- Pit-1 Related Gene Regulation --- p.19
Chapter 1.5.2 --- Regulation of Hormone Secretion --- p.21
Chapter 1.5.2.1 --- Hypothalamic Factors --- p.21
Chapter 1.5.2.2 --- Steroids --- p.23
Chapter 1.6 --- Aims of Thesis --- p.23
Chapter 1.6.1 --- Identification of SLII from Goldfish (Carassius auratus) --- p.23
Chapter 1.6.2 --- Aims --- p.27
Chapter CHAPTER 2 --- PCR ANALYSIS OF GFSLII GENE AND ITS EXPRESSION IN GOLDFISH TISSUE
Chapter 2.1 --- Introduction --- p.28
Chapter 2.2 --- Materials and Methods --- p.31
Chapter 2.2.1 --- Materials --- p.31
Chapter 2.2.2 --- Methods --- p.33
Chapter 2.2.2.1 --- Subcloning and DNA Sequencing of the Goldfish SLII Amplified by PCR --- p.33
Chapter 2.2.2.1.1 --- PCR Cloning of Goldfish SLII Gene --- p.33
Chapter 2.2.2.1.2 --- Restriction Enzyme Digestion of the PCR Clones --- p.33
Chapter 2.2.2.1.3 --- Subcloning of the Digested Fragments --- p.33
Chapter 2.2.2.1.4 --- DNA Sequencing of the Subcloned Fragments --- p.34
Chapter 2.2.2.2 --- Tissue Distribution Studies Using RNA Assay --- p.35
Chapter 2.2.2.2.1 --- Tissue Preparation --- p.35
Chapter 2.2.2.2.2 --- Total RNA Extraction --- p.35
Chapter 2.2.2.2.3 --- Electrophoresis of RNA in Formadehyde Agarose Gel --- p.36
Chapter 2.2.2.2.4 --- First Strand cDNA Synthesis --- p.37
Chapter 2.2.2.2.5 --- Goldfish SLII Specific PCR --- p.37
Chapter 2.2.2.2.6 --- PCR to Test DNA Contamination --- p.38
Chapter 2.3 --- Results --- p.39
Chapter 2.3.1 --- Subcloning and DNA Sequencing of the Goldfish SLII Amplified by PCR --- p.39
Chapter 2.3.2 --- Tissue Distribution Studies Using RNA Assay --- p.40
Chapter 2.4 --- Discussion --- p.45
Chapter 2.4.1 --- Subcloning and DNA Sequencing of the Goldfish SLII Amplified by PCR --- p.45
Chapter 2.4.2 --- Tissue Distribution Studies Using RNA Assay --- p.46
Chapter CHAPTER 3 --- ANALYSIS OF GOLDFISH SLII GENE
Chapter 3.1 --- Introduction --- p.47
Chapter 3.2 --- Materials and Methods --- p.49
Chapter 3.2.1 --- Materials --- p.49
Chapter 3.2.2 --- Methods --- p.54
Chapter 3.2.2.1 --- Screening of Goldfish Genomic Library --- p.54
Chapter 3.2.2.1.1 --- Preparation of the Plating Host --- p.54
Chapter 3.2.2.1.2 --- Preparation of the Probe --- p.54
Chapter 3.2.2.1.3 --- Primary Screening of Goldfish Genomic Library --- p.55
Chapter 3.2.2.1.4 --- Isolation of the Positive Clones --- p.56
Chapter 3.2.2.1.5 --- Phage Titering --- p.56
Chapter 3.2.2.1.6 --- Purification of the Positive Clones --- p.57
Chapter 3.2.2.1.7 --- Phage DNA Preparation --- p.57
Chapter 3.2.2.1.8 --- Find out the Target Gene Size of the Positive Clones --- p.58
Chapter 3.2.2.1.9 --- Cloning of the PCR Fragments into pUC18 Vector --- p.59
Chapter 3.2.2.1.10 --- Checking the Cloned Insert Size --- p.60
Chapter 3.2.2.1.11 --- Restriction Enzyme Digestion to Release the Inserts --- p.61
Chapter 3.2.2.1.12 --- Mini prep of the Positive Clones for Further Investigations --- p.61
Chapter 3.2.2.1.13 --- DNA Sequencing of the Positive Clones --- p.61
Chapter 3.2.2.1.14 --- Restriction Enzyme Mapping of the Positive Clones --- p.62
Chapter 3.2.2.1.15 --- Subcloning of Clone 2A and5A
Chapter 3.2.2.1.16 --- Determination of the Promoter Region of Clone 2A Using Universal Genome Walker Kit --- p.63
Chapter 3.2.2.2 --- Southern Blot Analysis of Goldfish and Catfish Genomic DNA --- p.66
Chapter 3.2.2.2.1 --- Genomic DNA Preparation from Goldfish and Catfish Tissues --- p.66
Chapter 3.2.2.2.2 --- Restriction Enzyme Digestion of the Genomic DNA --- p.67
Chapter 3.2.2.2.3 --- Alkaline Transfer of the Digested Genomic DNA --- p.67
Chapter 3.2.2.2.4 --- Hybridization of the Digested Genomic DNA --- p.67
Chapter 3.3 --- Results --- p.69
Chapter 3.3.1 --- Screening of the Goldfish Genomic Library --- p.69
Chapter 3.3.2 --- Mapping the Target Genes --- p.69
Chapter 3.3.3 --- DNA Sequencing of the 2 Positive Clones --- p.69
Chapter 3.3.4 --- Southern Blot Analysis of Goldfish and Catfish Genomic DNA --- p.81
Chapter 3.4 --- Discussion --- p.83
Chapter CHAPTER 4 --- EXPRESSION OF RECOMBINANT GOLDFISH SOMATOLACTIN IN ESCHERICHIA COLI (E. COLI)
Chapter 4.1 --- Introduction --- p.87
Chapter 4.2 --- Materials and Methods --- p.89
Chapter 4.2.1 --- Materials --- p.89
Chapter 4.2.2 --- Methods --- p.96
Chapter 4.2.2.1 --- Transformation of the Recombinant Protein Carrying Plasmid into E. coli. (BL21) --- p.96
Chapter 4.2.2.2 --- Small Scale Expression of Recombinant Goldfish SLII Protein --- p.96
Chapter 4.2.2.3 --- Large Scale Expression of Recombinant Goldfish SLII Protein --- p.97
Chapter 4.2.2.4 --- Preparation of the Recombinant Protein for Purification --- p.99
Chapter 4.2.2.5 --- Protein Purification Using Novagen His-Bind Resin Kit --- p.99
Chapter 4.2.2.6 --- Production of Polyclonal Antibody in Rabbits --- p.100
Chapter 4.2.2.7 --- Enzyme Linked Immunosorbant Assay (ELISA) --- p.101
Chapter 4.2.2.8 --- Western Blot Analysis of the Recombinant Hormones --- p.103
Chapter 4.3 --- Results --- p.105
Chapter 4.3.1 --- Expression of the Recombinant Goldfish SLII --- p.105
Chapter 4.3.2 --- Purification of the Recombinant Goldfish SLII --- p.105
Chapter 4.3.3 --- ELISA Analysis --- p.105
Chapter 4.3.4 --- Western Blot Analysis --- p.110
Chapter 4.4 --- Discussion --- p.113
Chapter 4.4.1 --- Expression of the Recombinant Goldfish SLII --- p.113
Chapter 4.4.2 --- Purification of the Recombinant Goldfish SLII --- p.114
Chapter 4.4.3 --- Analysis of the Recombinant Goldfish SLII --- p.114
Chapter CHAPTER 5 --- GENERAL DISCUSSION AND CONCLUSIONS --- p.116
REFERENCES --- p.123
Li, Cheng-Fang, et 李承芳. « Molecular cloning and analysis of Alterococcus agarase gene ». Thesis, 2005. http://ndltd.ncl.edu.tw/handle/94976591728616652853.
Texte intégral東吳大學
微生物學系
93
Agar is an important gelifying agent for biochemical use and the food industry, mainly extracted from the red seaweeds belonging to the family of Rhodophyceae. Agarose is the gelling component comprising a linear chain of alternating 3-O-linked-α-D-galactopyranose and 4-O-linked 3,6-anhydro-β-L-galactopyranose. A moderately thermophilic marine bacteria S3PY (lab code) which was isolated from hot springs in the intertidal zone of Lutao, Taiwan, produce at least 8 different extracellular agarases in molecular weight. That was Gram-negative halophile growing optimally at 0-5%NaCl. The optimal growth temperature range was approximate 50-55℃. The strains tolerated a relatively narrow pH range from 6 to 6.5 and had 64.5 mo1%G + C contents. Several physiology test and 16s rDNA sequence prove that S3PY was identical to the Alterococcus agarlyticus published by Shien in 1998. Partially Sau3A-digested genomic DNA of Alterococcus agarlyticus was cloned into BamHI-digested pUC19 vector and transformed to E.coli DH5α MCR‘. After 40,000-50,000 transformants were isolated, one with agarolytic activity clone was picked out. The pUC19 plasmid with a 5.2 kb insert harbored by the clone was designated as pUC3A. Multiple agarases expressed by the clone exactly match the agarases in S3PY, except 2-3Kda larger in molecular mass. SDS-PAGE containing 6M urea and 5 times of SDS showed those multiple agarases are individual monomer. Single open reading frame of 3,411bp was found and designated as agoI beginning with ATG at nucleotide 3409 (Met1) and ending with TAG at XbaI site of pUC19. Based on BLAST, the putative agarase gene belongs to GH 86; The search of Conserved domain shows that AgoI is consist of several module protein may served as substrate binding or surface adhesion. The functional fragments subcloning by PCR amplification implied those multiple agarases were originated from the single ORF agoI. The ORF subcloned in pET25b expression vector validate the comsuption. To further understand the regulation of Alterococcus Agarolyticus agarases, several hypotheses have been proposed.
« Molecular cloning of cellulase gene from volvariella volvacea ». Chinese University of Hong Kong, 1995. http://library.cuhk.edu.hk/record=b5888545.
Texte intégralThesis (M.Phil.)--Chinese University of Hong Kong, 1995.
Includes bibliographical references (leaves 112-114).
Abstract --- p.i
Acknowledgments --- p.iii
Table of contents --- p.v
Abbreviations --- p.x
List of figures --- p.xi
List of tables --- p.xiii
Chapter 1. --- Introduction
Chapter 1.1 --- General introduction --- p.1
Chapter 1.2 --- Purpose of study --- p.3
Chapter 2. --- Literature review
Chapter 2.1 --- Cellulose: properties and degradation --- p.4
Chapter 2.2 --- Cellulase system
Chapter 2.2.1 --- Definition and substrate specificity --- p.5
Chapter 2.2.2 --- Co-operation of cellulases --- p.5
Chapter 2.2.3 --- Multiplicity of cellulases --- p.6
Chapter 2.2.4 --- Regulation of cellulase synthesis --- p.6
Chapter 2.2.5 --- Architecture of cellulase protein --- p.8
Chapter 2.3 --- Molecular biology of fungal cellulase genes
Chapter 2.3.1 --- Structural organization of fungal cellulase genes --- p.15
Chapter 2.3.1.1 --- Promoter and regulatory sequence --- p.15
Chapter 2.3.1.2 --- Sequence at transcriptional start point (tsp) --- p.16
Chapter 2.3.1.3 --- Signal peptide --- p.18
Chapter 2.3.1.4 --- Intron --- p.18
Chapter 2.3.1.5 --- General sequence homology --- p.21
Chapter 2.3.2 --- Regulation of cellulase production at molecular level --- p.23
Chapter 2.3.3 --- Multiplicity of cellulase gene --- p.24
Chapter 2.3.4 --- Tactics to clone fungal cellulase genes --- p.25
Chapter 2.3.4.1 --- Past experience --- p.25
Chapter 2.3.4.2 --- Present approach --- p.28
Chapter 2.3.5 --- The importance of cellulase gene cloning --- p.29
Chapter 2.4 --- Cellulolytic microorganisms
Chapter 2.4.1 --- Ecological roles and diversity --- p.31
Chapter 2.4.2 --- "Biology of the straw mushroom, Volvariella volvacea" --- p.31
Chapter 3. --- Materials and methods
Chapter 3.1 --- Recipes of media and solutions
Chapter 3.1.1 --- Culture media and microbial-growth related chemicals --- p.34
Chapter 3.1.2 --- Solutions --- p.36
Chapter 3.2 --- Bacterial and fungal strains and the growth and storage of mycelium
Chapter 3.2.1 --- Bacterial and fungal strains --- p.42
Chapter 3.2.2 --- Growth and storage of mycelium --- p.42
Chapter 3.3 --- Extraction of DNA from mycelium --- p.43
Chapter 3.4 --- Degenerate polymerase chain reaction (PCR)
Chapter 3.4.1 --- Primers --- p.45
Chapter 3.4.2 --- Amplification conditions of degenerate PCR --- p.46
Chapter 3.5 --- Cloning of PCR products
Chapter 3.5.1 --- Ligation --- p.47
Chapter 3.5.2 --- Transformation --- p.47
Chapter 3.5.3 --- Screening by blue/white selection --- p.47
Chapter 3.5.4 --- Screening by PCR --- p.48
Chapter 3.6 --- Plasmid extraction by alkaline lysis
Chapter 3.6.1 --- Midi-preparation of plasmid by Qiagen column --- p.51
Chapter 3.6.2 --- Preparation of plasmid using Promega's Wizard minipreps DNA purification system --- p.51
Chapter 3.7 --- Sequencing analysis of cloned PCR products
Chapter 3.7.1 --- Growth and titering of helper phage R408 --- p.53
Chapter 3.7.1.1 --- Plate elution method --- p.53
Chapter 3.7.1.2 --- Liquid culture method --- p.53
Chapter 3.7.1.3 --- Titering of R408 --- p.53
Chapter 3.7.2 --- Rescue of single-stranded DNA from pCR-Script phagemid --- p.54
Chapter 3.7.3 --- Sequencing by chain-termination reaction --- p.54
Chapter 3.7.4 --- Preparation of polyacrylamide gel for DNA sequencing --- p.56
Chapter 3.7.5 --- Running a sequencing gel --- p.57
Chapter 3.7.6 --- "Fixation, exposure and development of sequencing gel and X-ray film" --- p.57
Chapter 3.7.7 --- Sequence analysis --- p.58
Chapter 3.8 --- Digestion of DNA with restriction enzymes --- p.59
Chapter 3.9 --- Agarose gel electrophoresis --- p.60
Chapter 3.10 --- Purification of DNA from agarose gel by Qiaex --- p.61
Chapter 3.11 --- Southern hybridization
Chapter 3.11.1 --- Southern blotting and DNA immobilization --- p.62
Chapter 3.11.2 --- Random-labelling of DNA probe and removal of unincorporated nucleotides --- p.63
Chapter 3.11.3 --- Pre-hybridization and hybridization --- p.63
Chapter 3.11.4 --- Exposure and development --- p.64
Chapter 3.11.5 --- Determination of molecular weight of hybridization signals --- p.65
Chapter 4. --- Results
Chapter 4.1 --- Extraction of DNA from the straw mushroom mycelium --- p.66
Chapter 4.2 --- Amplification of V. volvacea genomic DNA using degenerate primers --- p.70
Chapter 4.3 --- Cloning of PCR products using pCR-Script SK (+) cloning kit
Chapter 4.3.1 --- Screening by blue/white selection --- p.77
Chapter 4.3.2 --- Screening by PCR --- p.77
Chapter 4.4 --- Plasmid extraction by alkaline lysis --- p.80
Chapter 4.5 --- Preparation of single-stranded DNA template for sequencing
Chapter 4.5.1 --- Growth and titering of helper phage R408 --- p.82
Chapter 4.5.2 --- Rescue of single-stranded DNA from pCR-Script phagemid --- p.82
Chapter 4.6 --- Sequencing of cloned PCR products
Chapter 4.6.1 --- The choice of template --- p.84
Chapter 4.6.2 --- DNA and translated amino acid sequence of PCR clones --- p.84
Chapter 4.6.3 --- Alignment of DNA sequences against other fungal cellulase genes --- p.93
Chapter 4.6.4 --- Alignment of translated amino acid sequences against other fungal cellulase --- p.96
Chapter 4.7 --- Purification of DNA from agarose gel by Qiaex --- p.98
Chapter 4.8 --- Southern hybridization
Chapter 4.8.1 --- Restriction digestion of genomic DNA --- p.101
Chapter 4.8.2 --- Hybridization --- p.104
Chapter 5. --- Discussion
Chapter 5.1 --- Extraction of DNA from V. volvacea mycelium --- p.107
Chapter 5.2 --- Rationales of designing degenerate primers from heterologous amino acid sequence --- p.107
Chapter 5.3 --- Amplification of V. volvacea DNA using degenerate primers --- p.110
Chapter 5.4 --- Cloning of PCR products using pCR-Script system --- p.111
Chapter 5.5 --- The precaution of using Qiaex-purified DNA --- p.112
Chapter 5.6 --- Sequencing analysis
Chapter 5.6.1 --- DNA sequence analysis --- p.113
Chapter 5.6.2 --- Protein sequence analysis --- p.114
Chapter 5.7 --- Southern hybridization --- p.116
Chapter 6. --- Conclusion and further analysis --- p.117
Chapter 7. --- References --- p.119
HE, XING-FEN, et 何杏芬. « Molecular cloning of a □2 -adrenoceptor subtype gene ». Thesis, 1990. http://ndltd.ncl.edu.tw/handle/69343417531275585558.
Texte intégraliWu, Hsiao-we, et 吳孝薇. « Molecular cloning of lipoxygenase-like gene in rat ». Thesis, 2015. http://ndltd.ncl.edu.tw/handle/08421717447841218332.
Texte intégral慈濟大學
分子生物暨人類遺傳學系碩士班
103
A novel Lipoxygenase-like (Lipo-like) gene, was identified within the rat IEL rupture QTL region using various bioinformatics tools. Seven primer pairs were designed and RT-PCR from lung or spleen single strand cDNA demonstrated that rat Lipo-like gene encoded 762 amino acid residues with three putative PLAT (plasminogen activator) and one Fascin domains. Lipo-like gene consists of 20 exons with a CDS of 2289 bp, located in rat chromosome 5q11 region covering more than 246-kb. Lipo-like mRNA was highly expressed in lung, spleen, heart, muscle, and kidney; modestly expressed in liver, testis and large intestine; weakly expressed in stomach and small intestine but almost undetectable in brain, white blood cells or abdomental aorta. Two corresponding rat ESTs clones, CB326534.1 and CR464603.1, have been identified from NCBI EST Database. Attempts to order these two clones and sequenced were unsuccessful. Compared with SD rat CDS, BN has three point mutations, namely p. S432T, p. K492N and p. H592D. A homolog search in NCBI EST database further identified two human LIPO-like EST clones, AK316511.1 and AK304443.1 with 82% homology at the DNA level. The human homolog LIPO-like gene was successfully RT-PCR and cloned from H1299 cell line, a metastatic human lung cancer cell. Human LIPO-like gene is located at chromosomal 8q12.1, spanned about 210-kb with 24 exons of an ORF of 916 amino acid residues and a CDS of 2751 bp. Attempts to RT-PCR human LIPO-like in other cancer cells were unsuccessful. As PLAT domain has been implicated in cell migration and tissue remodeling whereas Fascin is a monomeric actin filament bundling protein and binds β-catenin, which suggested that this novel LIPO-like protein may play a role in cell-cell adhesion, cell migration or tissue remodeling.
YOU, ZHI-WEN, et 游志文. « Molecular cloning of Chinese hamster metallothionein-I gene ». Thesis, 1992. http://ndltd.ncl.edu.tw/handle/98111551591836149587.
Texte intégralWen, Hui-Ju, et 溫慧如. « Molecular cloning and expression of zebrafish single minded gene ». Thesis, 2000. http://ndltd.ncl.edu.tw/handle/49529384597426540684.
Texte intégral邵逸昕. « Molecular Cloning of the thdF Gene from Streptococcus Agalactiae ». Thesis, 1998. http://ndltd.ncl.edu.tw/handle/45663417275579734324.
Texte intégral國立臺灣大學
微生物學研究所
86
In the genus of streptococcus, Streptococcus agalactiae (group B streptococcus, GBS) is the only microorganism expressing hippuricase activity that can degrade hippuric acid into glycine and benzoic acid. In the attempt to isolate hippuricase gene (hipO) of GBS, a pair of primers were designed from the hipO gene nucleotide sequence of Camphylobacter jejuni. Using GBS genomic DNA as template, a 2.0 Kb DNA fragment was obtained by PCR. Then, the PCR product was labeled with digoxigenin-dUTP and used as a probe to screen GBS genomic library constructed with Lambda DASH Ⅱvector. A clone positively reacted with the probe was isolated and purified its DNA. The phage DNA fragment positively reacted with the probe was isolated and subcloned to pUC18 and sequenced. The nucleotide of the inserted DNA fragment was verified containing a partial thiophene degradation gene (thdF)fragment. Comparing amino acid sequence of thdF with that of other bacteria, the GBS gene had high homology with the gene of Escherichia coli, Bacillus subtilis, Borrelia burgdorferi, Haemophilus influenzae, Pseudomonas putida and Mycoplasm gentialium. By PCR study, thdF gene seems to be existed in some but not all GBS strains. Using the partial thdF gene containing recombinant plasmid to transform a thdF mutant strain E.coli NAR 967 for expression assay, none of transfornants was obtained recovering wild strain phenotype. Many questions are discussed in detail.
Chen, Tsung-Kun, et 陳宗坤. « Molecular cloning of eps8 gene from chicken embryonic cell ». Thesis, 1999. http://ndltd.ncl.edu.tw/handle/51481172423952507360.
Texte intégral國立成功大學
藥理學研究所
87
Previous studies have indicated that eps8 is one of the EGF receptor substrates and its overexpression can potentiate EGF-induced mitogenic effects. In v-Src transformed murine fibroblasts, two eps8 isoforms ( p97eps8 and p68eps8 ) can be recognized by anti-murine eps8 antibody generated in our laboratory previously. However, in chicken embryonic cells, a 200 kDa protein was detected by this antibody in the Western immunoblot analysis. In an attempt to confirm this 200 kDa protein was a chicken eps8 homologue, We screened a chicken embryonic cDNA library with 32P-labeled murine eps8 cDNA and got several positive clones. Sequence analysis of these chicken eps8-positive cDNA clones demonstrated the absence of the sequences at the 5' region. Utilizing 32P-labeled chicken eps8 cDNA as a probe, we demonstrated the molecular size of chicken eps8 mRNA was 4.7 Kb. And through 5'-RACE, we attempted to obtain the whole sequence of 4.7 kb chicken eps8 cDNA. Sequence comparison showed that eps8 cDNA from these two different species shared strong homology and both encoded 97 KDa protein. Thus, the detected 200 kDa protein in CE cell should represent a protein that shared the same antigen epitope present in mouse eps8. In addition, a rabbit polyclonal antibody specifically recognize chicken eps8 was attempted. Hopefully, with this antibody, we could study the interaction between v-Src and eps8 in CE cell.