Littérature scientifique sur le sujet « Miniaturized receptors »
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Articles de revues sur le sujet "Miniaturized receptors"
Hodder, Peter, Rebecca Mull, Jason Cassaday, Kurtis Berry et Berta Strulovici. « Miniaturization of Intracellular Calcium Functional Assays to 1536-Well Plate Format Using a Fluorometric Imaging Plate Reader ». Journal of Biomolecular Screening 9, no 5 (août 2004) : 417–26. http://dx.doi.org/10.1177/1087057104264038.
Texte intégralKlumpp, Martin, Andreas Scheel, Eloisa Lopez-Calle, Michael Busch, Kenneth J. Murray et Andrew J. Pope. « Ligand Binding to Transmembrane Receptors on Intact Cells or Membrane Vesicles Measured in a Homogeneous 1-Microliter Assay Format ». Journal of Biomolecular Screening 6, no 3 (juin 2001) : 159–70. http://dx.doi.org/10.1177/108705710100600306.
Texte intégralScheel, Andreas A., Bettina Funsch, Michael Busch, Gabriele Gradl, Johannes Pschorr et Martin J. Lohse. « Receptor-Ligand Interactions Studied with Homogeneous Fluorescence-Based Assays Suitable for Miniaturized Screening ». Journal of Biomolecular Screening 6, no 1 (février 2001) : 11–18. http://dx.doi.org/10.1177/108705710100600103.
Texte intégralXu, Chen-Yan, Kang-Ping Ning, Zheng Wang, Yao Yao, Qin Xu et Xiao-Ya Hu. « Flexible Electrochemical Platform Coupled with In Situ Prepared Synthetic Receptors for Sensitive Detection of Bisphenol A ». Biosensors 12, no 12 (25 novembre 2022) : 1076. http://dx.doi.org/10.3390/bios12121076.
Texte intégralKornienko, Oleg, Raul Lacson, Priya Kunapuli, Jonathan Schneeweis, Ira Hoffman, Todd Smith, Melissa Alberts, James Inglese et Berta Strulovici. « Miniaturization of Whole Live Cell-Based GPCR Assays Using Microdispensing and Detection Systems ». Journal of Biomolecular Screening 9, no 3 (avril 2004) : 186–95. http://dx.doi.org/10.1177/1087057103260070.
Texte intégralPolreichova, Miroslava, Usman Latif et Franz L. Dickert. « Functionalized Polymers as Receptors for Detection of Cells ». Australian Journal of Chemistry 64, no 9 (2011) : 1256. http://dx.doi.org/10.1071/ch11181.
Texte intégralIglesias, Alba, Sonia Lage, Maria Isabel Cadavid, Maria Isabel Loza et José Brea. « Development of a Multiplex Assay for Studying Functional Selectivity of Human Serotonin 5-HT2A Receptors and Identification of Active Compounds by High-Throughput Screening ». Journal of Biomolecular Screening 21, no 8 (10 juillet 2016) : 816–23. http://dx.doi.org/10.1177/1087057116644162.
Texte intégralLecas, Lucile, Lucie Hartmann, Lydia Caro, Sarah Mohamed-Bouteben, Claire Raingeval, Isabelle Krimm, Renaud Wagner, Vincent Dugas et Claire Demesmay. « Miniaturized weak affinity chromatography for ligand identification of nanodiscs-embedded G-protein coupled receptors ». Analytica Chimica Acta 1113 (mai 2020) : 26–35. http://dx.doi.org/10.1016/j.aca.2020.03.062.
Texte intégralCauteruccio, Silvia, Valentina Pelliccioli, Sara Grecchi, Roberto Cirilli, Emanuela Licandro et Serena Arnaboldi. « Bipolar Electrochemical Analysis of Chirality in Complex Media through Miniaturized Stereoselective Light-Emitting Systems ». Chemosensors 11, no 2 (13 février 2023) : 131. http://dx.doi.org/10.3390/chemosensors11020131.
Texte intégralRudiger, Martin, Ulrich Haupts, Keith J. Moore et Andrew J. Pope. « Single-Molecule Detection Technologies in Miniaturized High Throughput Screening : Binding Assays for G Protein-Coupled Receptors Using Fluorescence Intensity Distribution Analysis and Fluorescence Anisotropy ». Journal of Biomolecular Screening 6, no 1 (février 2001) : 29–37. http://dx.doi.org/10.1177/108705710100600105.
Texte intégralThèses sur le sujet "Miniaturized receptors"
Sinisi, Valentina. « Biomolecules as recognition elements for bioactive polyphenols in coffee ». Doctoral thesis, Università degli studi di Trieste, 2014. http://hdl.handle.net/10077/10135.
Texte intégralCoffee is a worldwide diffused beverage and it has a very high impact on the global economy. The success of coffee is due to the perfect combination between the overall involvement of our senses and the well-known stimulating effect. During the years, many studies focused on its volatile fraction, but our knowledge about the taste responsible compounds is still nowadays scarce. Among such compounds present in the coffee beans, chlorogenic acids (CGAs), that belong to the polyphenols family, acquire more and more importance, due to their key-role in determining the coffee flavor, particularly its bitterness, their beneficial health properties, and their possible use as marker to control the industrial process. The roasting of green coffee beans causes a partial loss of CGAs due to the formation of degradation products, such as the corresponding lactones, whose amount depends on the roasting degree; the identification and the quantification of acids and lactones in coffee could be therefore a useful parameter to evaluate the final quality of the beverage. This PhD project arises from the interest in searching new methodologies for the selective analysis of the lactone fraction, exploiting the recognition properties of biomolecules, proteins or peptides, or of designed sensing elements with high affinity for such lactonic compounds. The chosen molecules, not commercially available, were first synthesized and fully characterized, namely: 3,4-O-dicaffeoyl-1,5-gamma-quinide, 3-O-[3,4-(dimethoxy)cinnamoyl]-1,5-gamma-quinide, 3,4-O-bis[3,4-(dimethoxy)cinnamoyl]-1,5-gamma-quinide, and 1,3,4-O-tris[3,4-(dimethoxy)cinnamoyl]-1,5-gamma-quinide. A direct synthesis of the tri-substituted quinide was also tuned up, starting from D-(-)-quinic acid and 3,4-dimethoxycinnamoyl chloride. Considering the potential biological activities of polyphenols, the antiviral properties of these compounds against many viruses have been also evaluated. A possible approach to develop a selective biosensor is to use natural peptide scaffolds, with stable and highly organized conformations, and reduce its dimensions down to the limit of receptor stability, exploiting even the randomization of the aminoacid within the binding site to improve its ligand properties. In this perspective, the specific binding constants to Human Serum Albumin (HSA), more exactly to its Sudlow site I, of caffeic acid, ferulic acid, 3,4-dimethoxycinnamic acid, 5-O-caffeoyl quinic acid and of the four synthesized quinides were measured in physiological conditions by fluorescence spectroscopy, reaching promising KD values, in the micromolar range; moreover 3,4-O-dicaffeoyl-1,5-gamma-quinide gave a peculiar result, showing a very interesting double binding in the same active site of the protein. The two diester quinides were also used to test, always by means of fluorescence spectroscopy, the binding ability of a functional 100 aminoacids fragment that replicates the binding site I of the whole protein: this peptide, called HSA100, has been obtained by our research group; even some mutants of HSA100 have been prepared and four of them, randomly chosen, were used in the fluorescence assays. The obtained binding constants with HSA100 and its four mutants remain in the micromolar range, close to those measured with the whole protein, suggesting that proper mutations could lead to a selective biosensor with high affinity for the quinides. The Surface Plasmon Resonance (SPR) technique could offer a way for the fast screening of the binding properties. In the perspective of setting up a SPR method to quickly select the hits of the mutants libraries, a linker-equipped quinide was synthesized and immobilized on a gold chip, then the binding with HSA was tested in this way. Considering the well-known interaction between polyphenols compounds, such as chlorogenic acid, and caffeine in aqueous solution, NMR titrations were performed to study the behavior of caffeine with the synthesized quinides. This kind of binding may be exploited to develop a selective biosensor for quinides by introducing several molecules of caffeine or other xanthines on a suitable scaffold.
XXVI Ciclo
1986
Pavan, Silvia. « Peptides as Recognition and Sensing Elements ». Doctoral thesis, Università degli studi di Trieste, 2013. http://hdl.handle.net/10077/8659.
Texte intégralThe project deals with the design and development of artificial receptors for monitoring of drugs used for the HIV “Anti-Retroviral Therapy” (ART). Efavirenz, a RT-inhibitor, and its analogues were synthesised to screen with several techniques (surface plasmon resonance, NMR, fluorescence and UV spectroscopy) the new receptors obtained from different approaches. One exploits computational tools to model peptides with high affinity for small molecules. These designed peptides will then be incorporated in polymeric matrix for biosensor purposes. Molecularly imprinted polymers with efavirenz-binding properties were performed during my experience at Queen Mary University of London. Another approach is based on miniaturized receptors obtained from the reduction of the known sequence of a natural receptor as human serum albumin.
XXV Ciclo
1984