Thèses sur le sujet « Microfluidic light scattering blood »
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Han, Jin-Hee. « Microfluidic Detection of Waterborne Pathogen through Light Scattering of Particle Immunoassays ». Diss., The University of Arizona, 2008. http://hdl.handle.net/10150/195971.
Texte intégralLucas, Lonnie J. « Detection of Light Scattering for Lab-On-A-Chip Immunoassays Using Optical Fibers ». Diss., The University of Arizona, 2007. http://hdl.handle.net/10150/193897.
Texte intégralKirillin, M. (Mikhail). « Optical coherence tomography of strongly scattering media ». Doctoral thesis, University of Oulu, 2008. http://urn.fi/urn:isbn:9789514287572.
Texte intégralStewart-James, Samantha Ann. « Development of a microfluidic flow cytometry platform with fluorescence and light scattering detection for the rapid characterization of circulating tumor cells ». Thesis, Kansas State University, 2015. http://hdl.handle.net/2097/19078.
Texte intégralDepartment of Chemistry
Christopher T. Culbertson
Circulating tumor cells (CTCs) have become a key component in the identification and treatment of cancer. Once dislodged from the main tumor, CTCs travel through the bloodstream and cause metastasis. Early detection and identification of these cells can help in the evaluation and prognosis of various types of cancer, as well as assisting in patient treatments by determining the spread of the disease. Here, a high-throughput microfluidic analysis technique is described that can efficiently detect and identify cells, with the specific identification of CTCs as a future application through fluorescent labeling in mind. As proof of principle, the device has been shown to detect and characterize individual human Jurkat (T-lymphocyte) cells at a rate of 100 cells/minute. The device employs micro-scale flow focusing to isolate individual cells. The cells are detected using both light scattering and laser-induced fluorescence to evaluate cell size and surface functionality.
Nonoyama, Akihisa. « Using Multiwavelength UV-Visible Spectroscopy for the Characterization of Red Blood Cells : An Investigation of Hypochromism ». [Tampa, Fla.] : University of South Florida, 2004. http://purl.fcla.edu/fcla/etd/SFE0000508.
Texte intégralSiegemund, Thomas. « Structure and properties of drug-loaded polymeric nanoparticles targeting β-amyloid ». Doctoral thesis, Universitätsbibliothek Leipzig, 2011. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-70212.
Texte intégralKotouček, Jan. « Příprava a charakterizace komplexních nanočástic s využitím zejména frakcionace v tokovém poli a pokročilých spektroskopických metod ». Doctoral thesis, Vysoké učení technické v Brně. Fakulta chemická, 2020. http://www.nusl.cz/ntk/nusl-432947.
Texte intégralYoung, Anthony M. « Investigation of Laser Speckle Contrast Imaging's Sensitivity to Flow ». Miami University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=miami153256524246362.
Texte intégralMalm, Mikaela. « Drug Analysis : Bioanalytical Method Development and Validation ». Doctoral thesis, Uppsala universitet, Analytisk kemi, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8547.
Texte intégralCastro, David. « Two-Phase Microfluidic Systems for High Throughput Quantification of Agglutination Assays ». Diss., 2018. http://hdl.handle.net/10754/627925.
Texte intégralPeitinho, David Jorge Ligeiro. « Interaction between Gold Nanoparticles and Blood Proteins to define Disease states ». Master's thesis, 2018. http://hdl.handle.net/10362/127585.
Texte intégralUma das mais estudadas áreas em Bionanotecnologia é a aplicação de Nanopartículas de Ouro (AuNPs). Estas possuem propriedades óticas e químicas únicas e interagem com proteínas e outras biomoléculas, formando camadas dinâmicas a superfícies (Coroa Proteica). Estas coroas são responsáveis pelo aumento da biocompatibilidade in vivo, e podem ser estudadas com múltiplas técnicas, podendo identificar perfis de doença específicos. Neste trabalho, AuNPs de 15 nm foram sintetizadas pelo método de Turkevich, e AuNPs de 40 nm foram fornecidas. Concentração e tamanho das nanopartículas foram determinadas por espectroscopia UV-Vis, usando o efeito de Ressonância Plasmónica de Superfície (SPR). Funcionalização da superfície foi executada com adição de ácido 11-mercaptoundecanoico (MUA) e um penta-péptido (CALNN), mantendo a carga global negative e aumentando a estabilidade. Bioconjugação com Albumina (BSA) e Fibrinogénio (Fib) de soro bovino, com pesos moleculares de 66 e 340 kDa, respetivamente, foi executada e caracterizada por Eletroforese em Gel de Agarose (AGE). Mobilidade eletroforética foi determinada usando análise de imagem e vídeo com o programa eReuss. As constantes de afinidade de adsorção foram determinadas usando as curvas de conjugação pelos resultados de AGE, com a equação do modelo de adsorção de Langmuir, e resultou em (1.5 ± 0.1)x 10-2 (AuNP-MUA) para a conjugação com BSA, e (51.2 ± 4.7)x 10-2 (AuNP-CALNN) e (34.3 ± 1.2) x 10-2 (AuNP-MUA) para a conjugação com Fib. Bioconjugação de AuNP-CALNN com BSA foi inconclusiva. Cenários de competição numa mistura de proteínas favoreceu o Fib sobre a BSA. A conjugação de AuNPs de 40 nm mostrou múltiplas constantes de adsorção de (3 ± 0.7) x 10-2 e (9.7 ± 2.2) x 10-4 respetivamente. O programa eReuss provou ser uma poderosa ferramenta de análise de imagens das corridas eletroforéticas, e a componente de análise de vídeo sugere uma forma inovadora de analisar estas experiências e extrair informação adicional sobre a estabilidade da Coroa Proteica. A análise de Fergusson e técnicas de dispersão de luz (DLS, NTA e ELS) foram executadas para determinar o tamanho hidrodinâmico e o Potencial-Zeta de bionanoconjugados.
Costa, Carlos Filipe Santos. « Interaction between gold nanoparticles and blood proteins to define disease states ». Master's thesis, 2019. http://hdl.handle.net/10362/94109.
Texte intégralGiza, Marta Serra. « A mixed computational modelling and experimental approach to the interaction between gold nanoparticles and blood proteins ». Master's thesis, 2016. http://hdl.handle.net/10362/23125.
Texte intégralParthasarathy, Ashwin Bharadwaj. « Quantitative cerebral blood flow measurement with Multi Exposure Speckle Imaging ». Thesis, 2010. http://hdl.handle.net/2152/ETD-UT-2010-05-996.
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(8775689), Luis Palacio. « SMALL ANGLE SCATTERING OF LARGE PROTEIN UNITS UNDER OSMOTIC STRESS ». Thesis, 2020.
Trouver le texte intégralSiegemund, Thomas. « Structure and properties of drug-loaded polymeric nanoparticles targeting β-amyloid ». Doctoral thesis, 2010. https://ul.qucosa.de/id/qucosa%3A11216.
Texte intégralΑποστολόπουλος, Γεώργιος. « Μέτρηση γεωμετρικών χαρακτηριστικών και αναλογίας μεγεθών ερυθρών αιμοσφαιρίων με ψηφιακή επεξεργασία της σκεδαζόμενης ηλεκτρομαγνητικής ακτινοβολίας ». Thesis, 2010. http://nemertes.lis.upatras.gr/jspui/handle/10889/4084.
Texte intégralThe aim of this PhD thesis is the development of digital image processing and pattern recognition methods to estimate biometric and diagnostic parameters using scattering phenomena in the visible and infrared spectrum. More concretely, several reverse scattering problems of EM radiation from a human, healthy and undistorted Red Blood Cell (RBC) is solved. Methods of estimation and recognition of geometrical characteristics of healthy and undistorted RBCs using simulating images are presented. The information retrieval process includes, features extraction using two-dimensional integral transforms, features normalization, and Neural Networks for estimation of three major RBC geometrical proper-ties. Using the same features set, a recognition system of the geometric characteristics of RBCs was developed and evaluated. The scattering images were created simulating the forward scattering problem of a plane electromagnetic wave using the Boundary Element Method, taking into account both axisymmetric geometry of the scatterer and the non-axisymmetric boundary conditions of the problem. Initially, the problem is solved at 632.8 nm and consequently the same problem was solved at 12 different wavelengths, from 432.8 to 1032.8 nm equally spaced. Also, a new device for acquisition of scattering images from RBCs-flow, consisting of a multi-color light source (Led) was proposed, for RBC size estimation and recognition. Finally, a system for the estimation of different RBCs concentration was developed when scattering images acquired using multiple scattering images acquired from multiple Leds and color filters. The system was evaluated using additive white regular noise.
Τρικοίλης, Ιωάννης. « Εύρεση γεωμετρικών χαρακτηριστικών ερυθρών αιμοσφαιρίων από εικόνες σκεδασμένου φωτός ». Thesis, 2010. http://nemertes.lis.upatras.gr/jspui/handle/10889/3696.
Texte intégralIn this thesis we study and implement methods of estimating the geometrical features of the human red blood cell from a set of simulated light scattering images produced by a He-Ne laser beam at 632.8 μm. Ιn first chapter an introduction to the properties and the characteristics of red blood cells are presented. Furthermore, we describe various abnormalities of erythrocytes and the until now used ways of detection. In second chapter the properties of electromagnetic radiation and the light scattering problem of EM radiation from human erythrocytes are presented. The third chapter consists of two parts. In first part we analyse the theory of neural networks and we describe the radial basis function neural network. Then, we describe the theoritical and mathematical background of the methods that we use for feature extraction which are Singular Value Decomposition (SVD), Angular Radial Transform and Gabor filters. In second part the solution of the inverse problem of light scattering is described. We present the methodology of the solution process in which we implement a Singular Value Decomposition approach, a shape descriptor with Angular Radial Transform and a homogenous texture descriptor which uses Gabor filters for the estimation of the geometrical characteristics and a RBF neural network for the classification of the erythrocytes. In the forth and last chapter the described methods are evaluated and we summarise the experimental results and conclusions that were extracted from this thesis.