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1
Pierini, Rachele <1982>. « Ricerche sulla desinenza del genitivo singolare tematico in Lineare B ». Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2011. http://amsdottorato.unibo.it/4226/1/pierini_rachele_tesi.pdf.
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Pierini, Rachele <1982>. « Ricerche sulla desinenza del genitivo singolare tematico in Lineare B ». Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2011. http://amsdottorato.unibo.it/4226/.
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Naldi, Ludovica Diletta. « La riduzione della varietà merceologica tramite piattaforme di prodotto : sviluppo di indicatori e di un modello di progettazione (Parte B) ». Master's thesis, Alma Mater Studiorum - Università di Bologna, 2022.
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Per padroneggiare la complessità produttiva e logistica derivante dall’espansione della gamma offerta, sempre più aziende manufatturiere scelgono di adottare strategie come la Delayed Product Differentiation (DPD) che si è dimostrata utile a mantenere alto il livello di servizio offerto, riducendo al contempo la varietà merceologica a stock. In particolare, il presente studio si concentra sull’implementazione della DPD attraverso lo stoccaggio di opportune piattaforme di prodotto dalle quali derivare le varianti al momento del bisogno. Dopo aver fornito un inquadramento generale dei concetti appena illustrati, viene presentato un innovativo modello lineare per la progettazione di tali piattaforme. Sulla base della gamma di prodotti da ottenere, questo strumento determina la migliore strategia produttiva da assegnare a ciascuna variante, tra Assembly-to-Order (ATO), DPD e Make-to-Stock (MTS), specificando al contempo il miglior numero e composizione delle piattaforme eventualmente utilizzate. L’obiettivo di fondo è la riduzione della varietà di prodotti finiti, semilavorati e componenti da mantenere a magazzino per assemblare i diversi prodotti finiti. Nella parte A di questa tesi si è sviluppato un insieme di indicatori che, analizzando la struttura dei cicli di lavorazione in input, permetta di identificare le situazioni che più si prestano all’utilizzo di piattaforme di prodotto. La validità di queste ipotesi iniziali ed i vantaggi derivanti dalla strategia di DPD, sono dimostrate attraverso l’applicazione del modello di ottimizzazione a due casi studio, ottenendo nel caso identificato come favorevole dagli indicatori una significativa riduzione delle tipologie di oggetti a magazzino rispetto all’utilizzo della strategia MTS. In ultimo, viene valutato l’impatto positivo della riduzione della varietà di prodotti o del numero di componenti utilizzati sul tempo computazionale necessario a risolvere in maniera ottima il modello.
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Judson, Anna Penelope. « The undeciphered signs of Linear B ». Thesis, University of Cambridge, 2017. https://www.repository.cam.ac.uk/handle/1810/265630.
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More than sixty years after Michael Ventris’ decipherment of the Linear B script, 14 of its 87 syllabic signs still have no sound-values assigned to them. This group of ‘undeciphered’ signs represent a significant gap in our ability not only to read the Linear B script, but also to understand its development and use. Chapter 1 of this thesis analyses the origins and usage of signs with known sound-values to establish what types of values are in principle most likely to be found amongst the undeciphered signs: this investigation also enables an exploration of the development of Linear B, its relationship with its parent script Linear A, and the motivating factors underlying the creation of new Linear B signs. Chapter 2 consists of studies of each individual undeciphered sign, including a palaeographic analysis of their forms, discussion of their corpus of attestations, and an examination of their prospects of decipherment and possible sound-values in the light of the results of Chapter 1. Finally, Chapter 3 employs this group of signs in a case-study to explore the potential of palaeographic analysis to contribute to our understanding of wider issues concerning the Linear B script and its context of use within the Mycenaean palaces. This case-study focuses in particular on two main uses of palaeography: as a means of assigning a relative chronology to Linear B texts, and as evidence for the reconstruction of the Mycenaean scribes’ administrative work and training.
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Tomas, Helena. « Understanding the transcription from Linear A to Linear B script ». Thesis, University of Oxford, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.397466.
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Salgarella, Ester. « Aegean linear script(s) : rethinking the relationship between Linear A and Linear B ». Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/283184.
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When does a 'continuum' become a divide? My research investigates the genetic relationship between Linear A and Linear B (henceforth LA, LB), two Bronze Age scripts attested on Crete and Mainland Greece and understood to have developed one straight out of the other. By using an interdisciplinary methodology, I integrated linguistic, epigraphic, palaeographic and archaeological evidence, and placed the writing practice in its socio-historical setting. By challenging traditional views, my work has called into question widespread assumptions and interpretative schemes on this relationship. I carried out a systematic assessment of the structural characteristics underlying both systems and a palaeographic examination of their sign inventories. Built on these analyses, I put forward a more fluid model of script development, which takes LA and LB no longer as two separate scripts but as the very same one: the 'Aegean Linear Script'. Over time, this underwent only minimal adaptation when required to meet the needs of another language, arguably in view of the changing socio-political context. This new interpretation is in sharp contrast with the view traditionally held of a strong standardisation process having taken place, which sees LB as mere product of secondary script development. My conclusion has interesting implications not only for the history of the writing tradition on Crete, but also for our appreciation of the contemporary socio-historical context. This view, in turns, opens up new perspectives on the ideology associated with the retention of a script, matters of identity and how identity was negotiated at the very moment when Cretans and Greek-speaking Mainlanders came into closer contact in the Late Bronze Age. I have also noted that in this period the same scenario depicting soft adaptation of motives is witnessed on the side of material culture, displaying meaningful interplay and amalgamation of both Mainland and Cretan traditions.
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Jinbo, Maro. « Imagens ultra-sônicas em modo-B com técnicas de abertura focal sintética - SAFT ». Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/3/3152/tde-12072007-183723/.
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Este trabalho trata do estudo e aplicação da técnica de abertura focal sintética (SAFT) na geração de imagens por ultra-som em modo-B com transdutores matriciais lineares, para aplicação em ensaios não destrutivos. Sinais em modo-A são adquiridos e armazenados correspondentes ao meio inspecionado. Implementaram-se duas estratégias de processamento baseadas no método SAFT. A primeira consiste em um elemento central do array emitindo e todos recebendo, resultando em imagens com uma baixa energia acústica envolvida. A outra estratégia considera cada um dos elementos do array emissores e receptores gerando imagens de melhor qualidade, mas requer um maior tempo de processamento. Aplicaram-se técnicas de processamento digital de sinais como: filtragem, extração da envoltória, retificação, compressão logarítmica, interpolação e janelas de ponderação. Arquivos de dados foram gerados com um array de 128 elementos pelos pesquisadores da Universidade de Michigan e disponibilizados no site. Esses foram utilizados para a elaboração e otimização dos algoritmos no MatlabR. As imagens obtidas foram analisadas com relação a alguns parâmetros como a resolução axial e lateral, o contraste e o tempo de processamento. Foi confeccionado, no Laboratório de Ultra-Som da Escola Politécnica da Universidade de São Paulo, transdutor array de 16 elementos. Realizaram-se experimentos, adquirindo-se sinais que foram processados, gerando imagens referentes ao meio inspecionado.This work deals with the study and application of the synthetic aperture focusing technique (SAFT) for generating ultrasonic images in mode-B with linear array transducers for nondestructive testing applications. Modo-A signals are acquired and stored corresponding to the inspected medium. It was implemented two processing strategies based on SAFT. The first one consists of an array emitting with the central element and receiving with all the elements, resulting in images with a low acoustic energy involved. The second considers each one of the emitting and receiving elements of the array, generating images of better quality but requiring a longer processing time. Digital signal processing techniques were applied such as: filtering, envelope detection, rectification, logarithmic compression, interpolation, and weighting windows. Data were generated with a 128-element array by researchers from the University of Michigan and made available at the institution website. These data were used for elaborating and optimizing the algorithms using MatlabR. The acquired images were analyzed regarding some parameters as axial and lateral resolution, contrast and the processing time. A 16-element array was confectioned in the Ultrasound Laboratory of the School of Engineering at the University of São Paulo. Experiments were conducted and signals were acquired and processed, resulting in images of the inspected medium.
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Linderson, Ylva. « Transcriptional mechanisms directing terminal differentiation of B lineage cells / ». Stockholm : [Karolinska institutets bibl.], 2002. http://diss.kib.ki.se/2002/91-7349-328-7/.
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Turchinovich, Gleb. « BKLF promotes B cell differentiation towards marginal zone lineage ». [S.l. : s.n.], 2007. http://nbn-resolving.de/urn:nbn:de:bsz:25-opus-44233.
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Tenner, Jana. « Die frühgriechische Linear B-Schrift System, Verwendung und Entzifferung / ». [S.l. : s.n.], 2003. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB11675543.
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Åhsberg, Josefine. « Molecular mechanisms in lymphoid restriction : securing the B lineage fate ». Doctoral thesis, Linköpings universitet, Avdelningen för mikrobiologi och molekylär medicin, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-114273.
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With the work in this thesis I have aimed to deepen the understanding of the mechanisms behind the development of different blood cell lineages with a specific focus on B cell development. To understand the interplay between extracellular signaling and transcription factor networks in early lymphoid development we investigated the functional collaborations of FLT3 and IL7R. We found that signaling via FLT3 and IL7R act in powerful synergy on proliferation of common lymphoid progenitors (CLPs). In addition to a role in expansion of progenitor cells we provided evidence for that IL7R signaling play a crucial role in B-cell commitment. IL7 deficient mice display a dramatic block in development before functional lineage restriction in the Ly6D+ CLP-compartment. The few Ly6D+CLPs that do develop have reduced mRNA levels of transcription factor EBF1, a protein with crucial functions in lineage restriction and activation of the B-lymphoid program. One crucial function of EBF1 is to activate Pax5. Even though Pax5 deficient fetal liver cells upon transplantation to congenic hosts will generate an abundance of cells with an activated B-lineage transcriptional program, the pro-B cells have disrupted regulation of non-B-lineage transcripts and a propensity to develop into T- and NK-cells in vitro. Both the activation of the B-lineage program and lineage restriction was dependent on the dose of transcription factors. Mice carrying a heterozygous mutation for the transcription factor E2A had slightly reduced relative frequency of progenitor cells and an impaired B-lineage specification in CLPs. Loss of one allele of Ebf1 resulted in reduced surface expression of IL2Rα and pre-B cell receptor (BCR), reduced IL7-response in vitro, and disrupted cell cycle dynamics in pro- and pre-B cells. While heterozygous loss of Pax5 did not result in any dramatic phenotype, the combined loss of one allele of Pax5 and one allele of Ebf1 (Pax5+/-Ebf1+/-) had a dramatic effect on lineage plasticity in B-cell progenitors compared to the single heterozygotes. Furthermore, these Pax5+/-Ebf1+/- mice developed spontaneous, transplantable pro-B cell tumors and had a significantly reduced probability to survive over time. The transformed cells show high in vitro plasticity and tumor cells with induced overexpression of intracellular Notch1 can transform into T-lineage cell in vivo. The data presented in this thesis add important pieces of information to the field of developmental hematopoiesis. By increasing the analytical depth of development in normal circumstances, and by understanding the consequence of genetic mutations in relation to cell type, we hope to contribute to the understanding of hematopoietic development in health and disease.
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Georgopoulos, John. « Observations on the phonetic structure of the Minoan Linear A script / ». Title page, abstract and contents only, 1996. http://web4.library.adelaide.edu.au/theses/09ARM/09armg352.pdf.
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Brown, Christopher. « Improved Access to New Hydroxamic Acid Macrocycles and Linear Analogues ». Thesis, University of Sydney, 2020. https://hdl.handle.net/2123/23917.
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The hydroxamic acid (HXA) functional group is a class of organic acid that forms stable complexes with a range of metal ions. The HXA group has been useful in therapeutic areas, such as iron chelation therapy and radiometal-immunological PET imaging. Multimeric HXA-containing compounds can adopt either macrocyclic or linear structural architectures, as comprised from endo-HXA monomeric subunits. Obtaining these endo-HXA monomers synthetically is difficult, due to low yields and inefficient reaction times. This creates issues when synthesising macrocyclic or linear products from these subunits and inhibits the ability to study these compounds in a therapeutic setting. One aim of this thesis was to revise the synthesis of the endo-HXA monomer. Subsequent to this, the endo-HXA monomers were deployed to create macrocyclic compounds using synthetic techniques, such as metal-templated synthesis (MTS) and metal-assisted ring closure (MARC). Linear constructs were produced using amide-coupling chemistry to graft an endo-HXA monomer onto the native HXA-containing desferrioxamine B (DFOB). This thesis improved the yield and efficiency of accessing endo-HXA monomers using a tailored protecting group strategy. Furthermore, endo-HXA monomer analogues were produced, which were used to create structural variation in macrocyclic and linear compounds. The endo-HXA monomers were used in an MTS approach to generate dimeric HXA macrocycles. This technique yielded analytical quantities of material. Access to these constructs was improved upon by using the MARC synthetic technique, which required the formation of one rather than two amide bonds. Linear constructs were created by amide coupling a variety of endo-HXA monomers to DFOB. These ligands could be useful in 89Zr immunological PET imaging. This thesis describes the improved synthesis of endo-HXA monomers that allowed the creation of unique macrocyclic and linear architectures that could be useful in a therapeutic setting.
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Gervazoni, Ellen Silva [UNESP]. « Análise de estabilidade linear de escoamentos bidimensionais do Fluido Oldroyd-B ». Universidade Estadual Paulista (UNESP), 2016. http://hdl.handle.net/11449/143463.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Diversos escoamentos de interesse prático são de fluidos viscoelásticos e muitas vezes é desejável saber se estes escoamentos propagam-se no estado laminar ou no turbulento. Embora a hidrodinâmica de fluidos viscoelásticos sejam fortemente afetadas pelo balanço entre forças inerciais e elásticas no escoamento, o efeito da elasticidade sobre a estabilidade de escoamentos inerciais não foi completamente estabelecida. No presente trabalho, estuda-se o que ocorre entre estes dois estados, na transição laminar-turbulenta. Especi- ficamente, é investigada a convecção de ondas de Tollmien-Schlichting para o escoamento incompressível de Poiseuille para um fluido viscoelástico, utilizando a equação constitutiva Oldroyd-B. Para isto, utiliza-se a Simulação Numérica Direta para verificar a estabilidade dos escoamentos de fluidos viscoelásticos a perturbações não estacionárias. Os resultados numéricos obtidos para escoamentos de fluidos viscoelásticos são comparados com os resultados de escoamentos de fluidos Newtonianos, que já estão bem documentados na comunidade científica. Além disso, uma equação de Orr-Sommerfeld modificada é deduzida para um escoamento viscoelástico utilizando a Teoria de Estabilidade Linear.
Several flows of practical interest are of viscoelastic fluids and it is often desirable to know if these flows are in a laminar or turbulent state. Although the hydrodynamics of viscoelastic fluids are strongly affected by the balance between inertia and elastic forces in the flow, the effect of elasticity on the stability of inertial flows has not been completely established. In this work is studied what happens between these two states, the laminar-turbulent transition. Specifically, it will be investigated the convection of Tollmien-Schlichting waves to incompressible Poiseuille flow of viscoelastic fluid, using the constitutive equation Oldroyd-B. For this, the analysis is carried out by means of Direct Numerical Simulation to verify the stability of the non-stationary disturbances viscoelastic fluids flows. The numerical results obtained for viscoelastic fluids flows are compared with the results of Newtonian fluids flows, which are already well documented in scientific community. In addition, an Orr-Sommerfeld modified equation is deducted for a viscoelastic flow using Linear Stability Theory.
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Gervazoni, Ellen Silva. « Análise de estabilidade linear de escoamentos bidimensionais do Fluido Oldroyd-B / ». Presidente Prudente, 2016. http://hdl.handle.net/11449/143463.
Texte intégralRésumé :
Orientador: Analice Costacurta BrandiBanca: Gilcilene Sanchez de Paulo
Banca: Leandro Franco de Souza
Resumo: Diversos escoamentos de interesse prático são de fluidos viscoelásticos e muitas vezes é desejável saber se estes escoamentos propagam-se no estado laminar ou no turbulento. Embora a hidrodinâmica de fluidos viscoelásticos sejam fortemente afetadas pelo balanço entre forças inerciais e elásticas no escoamento, o efeito da elasticidade sobre a estabilidade de escoamentos inerciais não foi completamente estabelecida. No presente trabalho, estuda-se o que ocorre entre estes dois estados, na transição laminar-turbulenta. Especi- ficamente, é investigada a convecção de ondas de Tollmien-Schlichting para o escoamento incompressível de Poiseuille para um fluido viscoelástico, utilizando a equação constitutiva Oldroyd-B. Para isto, utiliza-se a Simulação Numérica Direta para verificar a estabilidade dos escoamentos de fluidos viscoelásticos a perturbações não estacionárias. Os resultados numéricos obtidos para escoamentos de fluidos viscoelásticos são comparados com os resultados de escoamentos de fluidos Newtonianos, que já estão bem documentados na comunidade científica. Além disso, uma equação de Orr-Sommerfeld modificada é deduzida para um escoamento viscoelástico utilizando a Teoria de Estabilidade Linear
Abstract: Several flows of practical interest are of viscoelastic fluids and it is often desirable to know if these flows are in a laminar or turbulent state. Although the hydrodynamics of viscoelastic fluids are strongly affected by the balance between inertia and elastic forces in the flow, the effect of elasticity on the stability of inertial flows has not been completely established. In this work is studied what happens between these two states, the laminar-turbulent transition. Specifically, it will be investigated the convection of Tollmien-Schlichting waves to incompressible Poiseuille flow of viscoelastic fluid, using the constitutive equation Oldroyd-B. For this, the analysis is carried out by means of Direct Numerical Simulation to verify the stability of the non-stationary disturbances viscoelastic fluids flows. The numerical results obtained for viscoelastic fluids flows are compared with the results of Newtonian fluids flows, which are already well documented in scientific community. In addition, an Orr-Sommerfeld modified equation is deducted for a viscoelastic flow using Linear Stability Theory
Mestre
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Lacombe, Tyler. « The regulation of T/B lineage commitment by Lunatic Fringe and Notch1 ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ58824.pdf.
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Wilemon, Billy B. Jr. « Portable X-Ray Fluorescence Spectrometer Analysis of the Pylos Linear B Tablets ». Thesis, Mississippi State University, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10642684.
Texte intégralRésumé :
This thesis investigates similarities in the chemistry of the Linear B clay tablets and sealings found at the Palace of Nestor in Pylos, located in the western Peloponnese. These similarities, or lack thereof, provide clues to the flow of material goods in and out of the palace and therefore to the degree of centralization of the government. Over a thousand 3000 year-old clay tablets and sealings currently housed at the National Archaeological Museum in Athens were analyzed using a pXRF over the course of the summers of 2015 and 2016. The chemical compositions were analyzed statistically. Results of the study and the conclusions are presented here.
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Sriramagiri, Harsha. « B Spline Modelling of Non Linear Power Amplifiers based on LSNA measurememts ». The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1243536474.
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EFKLEIDOU, KALLIOPI. « SLAVERY AND DEPENDENT PERSONNEL IN THE LINEAR B ARCHIVES OF MAINLAND GREECE ». University of Cincinnati / OhioLINK, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1099923171.
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Hermanns, Volker. « Zur Existenz von Rechtsinversen linearer partieller Differentialoperatoren mit konstanten Koeffizienten auf Blocp, k(w)-Räumen [B-Omega-Räumen] ». [S.l.] : [s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=975806661.
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Arumugakani, Gururaj. « Phenotypic and functional characterization of B-lineage cells associated with relapse and response to B-cell depletion therapy for rheumatoid arthritis ». Thesis, University of Leeds, 2015. http://etheses.whiterose.ac.uk/10669/.
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Background: Clinical response to therapeutic B-cell depletion by anti-CD20 antibody in rheumatoid arthritis (RA) is not associated with robust depletion of disease specific anti-citrullinated protein antibodies (ACPA). This suggests pathogenesis mediated predominantly by short-lived antibody secreting cells or an antibody independent role of B-cells or both in RA. The persistence of ACPA post B-cell depletion is consistent with secretion from long-lived plasma cells (PC) and could be linked to non-response to B-cell depletion. The aim of this thesis was to delineate populations of B-cells and plasma cells linked to RA disease activity. Results: Autoreactive citrullinated protein-specific B-cells were detected using ELISA from supernatants of B cell culture but not by flow cytometry or ELISpot. RA patients relapsing following B-cell depletion showed an increased proportion of a memory B-cell subset in the peripheral blood. The in vitro stage of B-cell differentiation closest to this relapse associated subset secreted multiple proinflammatory mediators. A novel mode of contact-dependent B-NK cell interaction was noted, likely to be due to EBV latency in B-cells or a novel mode of B-cell regulation by NK cells. CD19Neg PCs had a longer recovery time following depletion and had a longer life span in an in vitro PC differentiation model system but were generated early, which suggests that CD19 negativity is a marker for potential to be long-lived rather than PC age. Conclusions: Memory B-cell subset distribution is skewed during clinical relapse in RA which reflects on-going B-cell activity/differentiation generating inflammatory mediators or pathogenic short-lived antibody secreting cells which explains response to B-cell depletion or anti-TNF therapy. In those patients where B-cell depletion does not achieve clinical response, CD19Neg long-lived PCs may have a pathogenic role. Agents targeting certain stages of B-cell differentiation or long-lived PCs can be therapeutic options in carefully selected patients.
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Aggarwal, Aditya Mohan. « B-Spline Boundary Element Method for Ships ». ScholarWorks@UNO, 2008. http://scholarworks.uno.edu/td/853.
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The development of a three dimensional B-Spline based method, which is suitable for the steady-state potential flow analysis of free surface piercing bodies in hydrodynamics, is presented. The method requires the B-Spline or Non Uniform Rational B-Spline (NURBS) representation of the body as an input. In order to solve for the unknown potential, the source surface, both for the body as well as the free surface, is represented by NURBS surfaces. The method does not require the body surface to be discritized into flat panels. Therefore, instead of a mere panel approximation, the exact body geometry is utilized for the computation. The technique does not use a free surface Green's function, which already satisfies the linear free surface boundary conditions, but uses a separate source patch for the free surface. By eliminating the use of a free surface Green's function, the method can be extended to considering non-linear free surface conditions, thus providing the possibility for wave resistance calculations. The method is first applied to the double body flow problem around a sphere and a Wigley hull. Some comparisons are made with exact solutions to validate the accuracy of the method. Results of linear free surface conditions are then presented.
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Steward, Colin Graham. « Technical aspects of minimal residual disease detection in childhood B-lineage acute lymphoblastic leukaemia ». Thesis, University of Bristol, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241085.
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Gouveia, Paulo D. F. « Codificação de fala por modelos variáveis no tempo ». Master's thesis, Universidade de Aveiro, 1996. http://hdl.handle.net/10198/1572.
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O trabalho apresentado nesta tese representa uma contribuição para a optimização da codificação da fala. Utilizam-se para o efeito modelos de codificação baseados em filtros LP (filtros de Predição Linear) de parâmetros variáveis no tempo, contrastando com os modelos fixos utilizados nos métodos convencionais. Nestes, a adaptação dos filtros de predição realiza-se simplesmente através de actualizações periódicas dos seus parâmetros, não traduzindo por isso uma evolução gradual e contínua ao longo do tempo.
A técnica utilizada na implementação dos modelos variáveis tem por base a utilização de funções do tipo B-spline na representação das formas de onda dos parâmetros LP.
Para o estudo da viabilidade do modelo proposto, analisou-se o desempenho de um vocoder de predição linear incluindo, quer o modelo LP de parâmetros variáveis, quer o modelo LP de parâmetros fixos convencional, por forma a possibilitar a comparação de desempenhos.
Dos resultados obtidos concluímos que a codificação de fala por modelos variáveis no tempo, embora não tenha evidenciado vantagens convincentes, pode ser encarada como outra forma de codificação, competindo por isso com as metodologias já existentes. The work presented in this thesis aims at to be a contribution to speech coding. To accomplish this objective, coding models based on LP filters (Linear Predictive Filters) with time-varying parameters are used, and compared with fixed models used in conventional methods. In these models, the predictive filters adaptation is carried on simply through periodic updatings of its parameters, therefore doesn’t representing a gradual and continuous evolution in time.
The technique used in varying models implementation is based on the utilization of B-spline like functions to represent the LP parameters waveforms.
In order to make a viability study of the proposed model, the performance of a linear predictive vocoder was analyzed, including both the LP model with varying parameters and the conventional LP model with fixed parameters, thus enabling the comparison of their performances.
From the results, we concluded that speech coding by time-varying models, although it had not demonstrated clear benefits, can be viewed as another coding way, therefore competing with the already existing methodologies.
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Magoon, Jason. « Application of the B-spline collocation method to a geometrically non-linear beam problem / ». Online version of thesis, 2010. http://hdl.handle.net/1850/11587.
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Morla, Ricardo Santos. « Comutação de solitões em fibras ópticas não-lineares ». Dissertação, Lisboa : edição do autor, 2001. http://catalogo.up.pt/F?func=find-b&local_base=FCB01&find_code=SYS&request=000011415.
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Kim, Hak Lim Nancy. « Regulation and deletion of early B lineage precursor cells (pro-B cells) in the bone marrow of mice with severe combined immunodeficiency (SCID) ». Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=68188.
Texte intégralRésumé :
Mice homozygous for the scid (severe combined immunodeficiency) mutation are generally unable to produce B lymphocytes, a condition attributed to defective rearrangements of immunoglobulin genes in precursor B cells. Some early B lineage cells are present in the bone marrow (BM), however. In scid mice, we defined three subsets of early progenitor B cells lacking $ mu$ heavy chains (pro-B cells) based on the expression of terminal deoxynucleotidyl transferase (TdT) and B220 glycoprotein: (a) early (TdT+B220$-$), (b) intermediate (TdT+B220+), and (c) late (TdT$-$B220+). Double immunofluorescence labelling of BM cell suspensions has shown normal numbers of early and intermediate pro-B cells, substantially reduced numbers of late pro-B cells, and an absence of pre-B cells and B cells. Nearly all defective cells, thus, abort at the late pro-B cell stage. Early and intermediate pro-B cells accumulated in metaphase in near-normal numbers after intraperitoneal vincristine administration, apparently not regulated by feedback signals from the B cell pool.The scid mouse provided a system for studies on regulation of pro-B cells and the effectiveness of the deletion mechanism. It was determined that the defective pro-B cells of scid mice were susceptible to the stimulatory effects of an exogenous agent, sheep red blood cells, and a stromal cell-derived growth factor for precursor B cells, interleukin-7, as previously observed in normal mice in vivo. In addition, pro-B cells of scid mice were driven to proliferate by the dysregulated constitutive expression of the c-myc oncogene. In each case, however, all cells aborted soon after reaching the late pro-B stage. Mechanisms by which aberrant cells are deleted from the B lineage, safeguarding against entry of potentially dysregulated cells into the peripheral B cell pool, thus, could not be overwhelmed or evaded by increased proliferative activity of pro-B cells.
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Varias, García Carlos. « Los documentos en lineal B de Micenas. Ensayo de interpretación global ». Doctoral thesis, Universitat Autònoma de Barcelona, 1993. http://hdl.handle.net/10803/5550.
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Un estudio de las 65 tablillas y 8 nódulos de arcilla en lineal b procedentes de Micenas (Grecia), datados entre 1250 y 1200 a.c., a partir de la última edición de los textos: -Olivier, J.-P. y Melena, J.L., Tithemy, Salamanca 1991, intenta esclarecer el funcionamiento del palacio de Micenas en el siglo XIII a.c. El estudio combina los análisis arqueológico y filológico. Los cuatro primeros capítulos analizan los cuatro establecimientos palaciegos en donde se han hallado el grueso de los textos: 1/casa del comerciante de aceite, con registros de aceite y lana, 2/ casa de las esfinges, con registros de especias y vasos; 3/casa occidental, que funciona como alojamiento y manutención de fuerzas de trabajo; 4/casa de la ciudadela, que es la zona de culto religioso. Cuatro tablillas aisladas son examinadas en el capítulo V. En las conclusiones abordamos el examen paleográfico de los textos, la descripción de la lengua de Micenas, la composición de su población a partir de los antroponimos y el sistema de archivos de este reino.
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Lowenstein, Marcia. « Interactions between Endothelin Receptor B and Transcription Factors Sox10 and Pax3 in the Melanocyte Lineage ». FIU Digital Commons, 2009. http://digitalcommons.fiu.edu/etd/117.
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Genetic interactions that underlie developmental processes such as cell differentiation and pattern formation are complex and difficult to elucidate. Neural Crest (NC) cells and their derivatives offer an optimal system in which to probe for these complex interactions as they acquire different cell fates and constitute a variety of structures. The transcription factors Sox10 and Pax3 as well as the transmembrane receptor Endothelin receptor b (Ednrb) are temporally and spatially co-expressed early in NC cells and mutations in these genes lead to similar hypopigmentation phenotypes due to a reduced number of NC-derived melanocyte precursors, the melanoblasts. The goal of this study was to establish whether Sox10 and Ednrb or Pax3 and Ednrb interact to promote normal murine melanocyte development. Crosses of Sox10 or Pax3 with Ednrb heterozygous mutants showed that the double heterozygous hypopigmentation phenotype was significantly more pronounced than phenotypes of single heterozygotes, implying that a synergistic interaction exists between Sox10 and Ednrb and Pax3 and Ednrb. This interaction was further explored by the attempt to rescue the Sox10 and Pax3 hypopigmentation phenotypes by the transgenic addition of Ednrb to melanoblasts. Pigmentation was completely restored in the Sox10 and partially restored in the Pax3 mutant mice. The comparison of the number of melanoblasts in transgenic and non-transgenic Sox10 mutant embryos showed that the transgenic rescue occurred as early as E11.5, a critical time for melanoblast population expansion. Cell survival assays indicated that the rescue was not due to an effect of the transgene on melanoblast survival. A novel phenotype arose when studying the interaction between Ednrb and Pax3. Newborns appeared normal but by 3.5 weeks of age, the affected pups were smaller than normal littermates and developed a dome-shaped head; some also developed thoracic kyphosis. Affected pups were dead by 4 weeks of age: 80% were Pax3Sp/+ and 75% were female. When compared to normal littermates, affected mice had brains with enlarged 4th ventricles and more glia while skeletal staining showed kyphosis, wider rib cages and pelvic differences. An epistatic interaction resulting from the mixing of genetic backgrounds that is exacerbated in the presence of Pax3 heterozygosity is suspected.
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Morales, Carmen. « Detection of IL4 and TNFÃ and their receptors in chronic lymphocytic leukemia of B lineage ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/MQ62801.pdf.
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Venkitaraman, Ashok Ramakrishnan. « The regulation of MHC class 2 gene expression by tumours of the B lymphocyte lineage ». Thesis, Imperial College London, 1989. http://hdl.handle.net/10044/1/47696.
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Whelan, Jarrett Bertrand Fred. « Enforced Expression of Hoxa9 in B-Lineage All Promotes Survival and Proliferation of Leukemic Cells ». [Greenville, N.C.] : East Carolina University, 2009. http://hdl.handle.net/10342/1903.
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Weathersby, Stephen. « Damping higher order modes in the PEP-II B-factory storage ring collider ». Diss., Connect to online resource - MSU authorized users, 2007.
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Thesis (Ph.D.)--Michigan State University. Dept. of Physics and Astronomy, 2007.Title from PDF t.p. (viewed on August 18, 2009) Includes bibliographic references (p. 175-179). Also issued in print.
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Meng, Lingzhang [Verfasser]. « The impact of B-lineage derived IL-10 on fate decision of monocyte differentiation / Lingzhang Meng ». Lübeck : Zentrale Hochschulbibliothek Lübeck, 2016. http://d-nb.info/1096832577/34.
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Aurora, Federico. « Chronological and geographical annotations in DAMOS ». Universitätsbibliothek Leipzig, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-221476.
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DAMOS is an online annotated database (MySql) of all published texts of Mycenaean, the earliest attested Greek dialect. The texts are annotated for epigraphical and linguistic features (morphology, syntax, semantics) and provided with a rich set of metadata, which also include chronological and geographical data. Genre (administrative accounts) and physical features (brevity and often fragmentary state) of the Mycenaean texts, and especially their script (Linear B), not well suited for rendering the
Greek language, pose challenges to the interpretation of the texts, which often result in multiple possible values of the data at all levels – epigraphical, linguistic, metadata. These may often be organized in competing sets of values, which form coherent different overarching hypotheses on e.g. the grammar of the language or the dating of an archive. These competing values need, thus, to be stored and meaningfully organized in the database. The presentation focuses on how chronological and geographical data (both about the texts and contained in the texts) and their often multiple possible values are dealt with in the arrangement of the database structure of DAMOS.
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Lima, Marcelo de. « Mapeamento e deleção de epítopos lineares de linfócitos B em proteínas do vírus da síndrome respiratória e reprodutiva dos suínos para a produção de uma vacina diferencial ». Universidade Federal de Santa Maria, 2008. http://repositorio.ufsm.br/handle/1/4033.
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Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorPorcine reproductive and respiratory syndrome virus (PRRSV) was isolated for the first time in 1991 and since then it has been associated with significant economic losses to the pig industry worldwide. Although vaccination against PRRSV is widely used, an important advance would be the development of marker vaccines allowing serologic discrimination between vaccinated and naturally infected animals. The present study aimed to identify immunogenic and conserved regions dispensable to viral replication in different PRRSV proteins, which could be used as negative serologic markers in a new generation of liveattenuated vaccines. A fine mapping of B-cell linear epitopes in different PRRSV proteins by Pepscan is presented in the first part of this thesis. The results indicated the presence of several B-cell linear epitopes in the non-structural protein 2 (Nsp2) and in all structural proteins encoded by PRRSV, which were consistently recognized by antibodies raised in pigs experimentally infected with a North American strain of the virus (NVSL97-7895). The Nsp2 was found to harbor the highest frequency of immunodominant epitopes (n=18) when compared to structural proteins. In the structural proteins, epitopes consistently recognized by immune sera were located in all studied proteins. Overall, the highest degree of immunogenicity and conservation was exhibited by two epitopes identified in the C-terminal end of the M protein (ORF6). The antibodies recognizing the immunodominant epitopes of each protein were detected as early as days 7 to 15 post-infection (p.i.) and remained detectable until the end of the experiment (day 90 p.i). Based on their immunodominance and level of amino acid (aa) conservation, two target epitopes were selected to serve as serological marker candidates in each of the following PRRSV proteins: Nsp2, GP3 and M. These epitopes were deleted in the wild-type cDNA infectious clone (FL-12) by site-directed mutagenesis. The results of this study are presented in the second part of this thesis. A Nsp2 mutant virus (FLdNsp2/44) was successfully rescued following RNA transfection in MARC 145 cells. This epitope deletion mutant fulfilled the requirements for a differential vaccine virus such as efficient growth in vitro and in vivo and induction of active seroconversion as measured by a commercial ELISA kit associated with the absence of a marker-specific peptide-ELISA response in 100% (n=15) of the vaccinated animals. In vitro and in vivo characterization of the mutant virus clearly showed that removal of a 15-mer Nsp2 epitope had no effect on the immunogenicity, growth properties or virulence when compared to the wild type virus. On the other hand, deletions of previously identified peptide marker candidates within GP3 and M genes were shown to be lethal for virus viability in vitro. Alternatively, by substitution of 5aa at a time within a M peptide marker candidate, a viable mutant virus could be recovered although it still resulted in a positive marker virus. In summary, our results provide proof of concept that PRRSV marker vaccines can be developed using such methodology. Taken together, these data indicate that the combination of a mutant virus carrying a deletion of an immunodominant epitope and the corresponding peptide ELISA represents an attractive approach for the development of PRRSV differential modified-live vaccines.
O vírus da síndrome respiratória e reprodutiva dos suínos (PRRSV) foi isolado pela primeira vez em 1991 e, desde então, tem sido associado a perdas significativas para a suinocultura mundial. Apesar da vacinação contra o PRRSV ser amplamente utilizada, um grande avanço seria alcançado com a elaboração de vacinas diferenciais que permitam a discriminação sorológica entre animais vacinados e naturalmente infectados. O presente estudo teve como objetivo a identificação de regiões imunogênicas, conservadas e dispensáveis a replicação viral, em diferentes proteínas do PRRSV, que pudessem ser utilizadas como marcadores sorológicos negativos em uma nova geração de vacinas atenuadas. Na primeira parte desta tese estão apresentados os resultados de um mapeamento de epítopos lineares de linfócitos B em diferentes proteínas do PRRSV, pelo uso da tecnologia de Pepscan. Os resultados indicam a presença de diversas regiões imunodominantes na proteína não estrutural 2 (Nsp2) e em todas as proteínas estruturais do vírus. Essas regiões foram consistentemente reconhecidas pelo soro de suínos experimentalmente infectados com uma cepa norte-americana do PRRSV (NVSL97-7895). A maior freqüência de epítopos imunodominantes foi identificada na Nsp2 (n=18) e o mais alto grau de imunogenicidade e nível de conservação de aminoácidos foi observado em dois epítopos identificados na extremidade carboxi-terminal da proteína M (ORF6). Anticorpos reagentes com epítopos imunodominantes de cada proteína foram detectados inicialmente entre os dias 7-15 pós-infecção (pi), permanecendo em altos títulos até o final do experimento (dia 90 pi). Com base na imunodominância e nível de conservação de amino ácidos (aa) das seqüências mapeadas, dois epítopos alvos foram selecionados como candidatos a marcadores sorológicos negativos em cada uma das proteínas Nsp2, Gp3 e M. Esses epítopos foram então deletados em um clone infeccioso de cDNA (FL12) por mutagênese sítio-direcionada. Os resultados desses experimentos encontram-se descritos na segunda parte da tese. Um vírus mutante carreando a deleção de um epítopo imunodominante da Nsp2 (FLdNsp2/44) foi obtido após transfeccção de RNA viral em células MARC145. A caracterização in vitro e in vivo do vírus mutante demonstrou que a remoção dos 15 aa da Nsp2 não produziu efeito sobre a imunogenicidade, replicação ou virulência quando comparado ao vírus parental. Além disso, observou-se indução de soroconversão contra o PRRSV em animais infectados, detectada pelo uso de um teste ELISA comercial. Por outro lado, não foi detectada resposta humoral específica contra a região deletada nos animais imunizados com o FLdNsp2/44, conforme resultados de um teste ELISA contendo como antígeno um peptídeo sintético correspondente a seqüência removida. Por outro lado, deleções dos epítopos previamente identificados na Gp3 e proteína M foram letais à viabilidade viral in vitro. Alternativamente, um outro vírus mutante foi gerado pela substituição de 5 aa do epítopo identificado na proteína M, embora a alteração de resíduos não tenha sido suficiente para eliminar a imunogenicidade da região. Em resumo, os resultados do presente estudo se constituem em uma prova de conceito no sentido do desenvolvimento de vacinas diferenciais contra o PRRSV. A utilização de um vírus mutante carreando a deleção de um epítopo imunodominante, associado com um teste de ELISA baseado no peptídeo sintético correspondente a região deletada, representam uma alternativa para o desenvolvimento de vacinas diferenciais atenuadas contra o PRRSV.
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Trabuco, D´orea Carlos Eduardo. « Sur l'(A, B)-invariance de polyèdres convexes ; application à la commande sous contraintes et au problème l1 ». Toulouse 3, 1997. https://tel.archives-ouvertes.fr/tel-00010088.
Texte intégralRésumé :
Ce travail porte sur l'étude de la propriété d'(A,B)-invariance de polyèdres convexes et son application à la commande sous contraintes et au problème l1. D'abord, nous proposons une caractérisation explicite de l'(A,B)-invariance de polyèdres convexes pour des systèmes en temps discret. Cette caractérisation se traduit par des conditions nécessaires et suffisantes sous la forme de relations matricielles linéaires, et présente deux avantages majeurs vis-à-vis de celles rencontrées dans la littérature : elle s'applique à tous les polyèdres convexes et elle ne nécessite pas le calcul de sommets. Ces avantages se font sentir notamment dans le calcul du domaine (a,b)-invariant supremal inclus dans un polyèdre donne, pour lequel nous proposons une méthode numérique. Le problème de calculer une loi de commande rendant positivement invariant en boucle fermée un polyèdre (a,b)-invariant est également traite. Les relations d'(A,B)-invariance sont alors généralisées à des systèmes soumis à des contraintes linéaires sur la commande et à des systèmes soumis à des perturbations additives bornées. Puis, les résultats obtenus en temps discret sont étendus aux systèmes en temps continu. Ensuite, le problème d'atténuation de perturbations additives persistantes, connu dans la littérature comme problème l1, est étudié. Les domaines (A,B)-invariants intérieurement stabilisables sont d'abord caractérises. Puis, nous proposons une approche décomposée pour le calcul du domaine intérieurement stabilisable supremal inclus dans le polyèdre défini par les contraintes de performance l1. Un niveau de performance donne est atteignable si et seulement si ce domaine supremal n'est pas vide. Cette approche géométrique permet notamment de déterminer directement la solution du problème l1 pour une classe importante de systèmes. Enfin, nous étendons l'étude de l'(A,B)-invariance de polyèdres à des systèmes dont le modèle est soumis à des incertitudes du type structure.
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Palmer, Ruth. « Wine in the Mycenaean palace economy / ». [S.l.] : Liège, 1994. http://catalogue.bnf.fr/ark:/12148/cb37474740m.
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Stavrianopoulou, Eftychia. « Untersuchungen zur Struktur des Reiches von Pylos : die Stellung der Ortschaften im Lichte der Linear B-Texte / ». Partille : P. Ǻströms, 1989. http://catalogue.bnf.fr/ark:/12148/cb388940220.
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Wilson, Nicola Kaye. « The mechanism of repression of the myeloid-specific c-fms gene by Pax5 during B lineage restriction ». Thesis, University of Leeds, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.485916.
Texte intégralRésumé :
The macrophage colony stimulating factor receptor encoded by the csf1r (formally known as c-fms) gene is a tyrosine kinase receptor which is essential for macrophage differentiation. This gene is upregulated during macrophage differentiation, but silenced in all non-macrophage cells. Previous studies have looked at the activation of csf1r within the myeloid lineage by investigating alterations in the ~hromatin structure of its cis-regulatory elements. However, the mechanism of repression is not fully understood.. ~My thesis examines the repression of csf1r in B cells. It was previously know that the trans~iption factor Pax5 is necessary forcsf1r repression. This thesis shows that Pax5 directly interacts with the csf1r promoter in a sequence specific manner and that the Pax5 deoxyribonucleic acid (DNA) binding domain alone is sufficient ~o repress csf1r. In addition, the recruitment of Pax5 does not lead to major alterations in the histone modification pr.ofile. This demonstrates tliat the mechanism of repression does not require the recruitment of chromatin modifying factors. The Pax5 binding site within the csf1r promoter overlaps with the region containing the major transcription start sites. This suggests that Pax5 represses csf1r by directly competing with the basal transcription machinery, rather than via epigenetic alterations of the chromatin. Besides the promoter, Pax5 interacts with the fms intronic regulatory element (FIRE). FIRE does not add a repressive activity to that of the csf1r promoter. Moreover, efforts to identify a Pax5 binding site on FIRE showed that Pc1lx5 does not directly bind to DNA, suggesting that the interaction was mediated by proteinprotein interactions. in vitro DNA-protein interaction studies were therefore carrried out, in this thesis and other studies, with nuclear extracts from myeloid cells and B cells to identify potential partners of Pax5. These experiments identified factors such as specificity protein 1 (Sp1), early growth factor 2 (Egr-2), PU.1, Runx 1 and CCAAT enhancer binding protein (C/EBP) bjnding to FIRE with both extracts, as well as several unidentified factors which could be potential interaction partners for Pax5. Antisense transcription start sites were identified in a previous study to be originating from FIRE. This thesis investigates the effect(s) of Pax5 on antisense promoter activity and clearly show that Pax5 is able to transactivate antisense promoter activity in B cells. Although the function of these antisense transcripts is currently unknown the elucidation of their regulation will potentially lead to the unveiling of their role during haematopoietic differentiation.
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King, R. Glenn. « On the immunological roles of TLT2 and HSH2 ». Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2007. https://www.mhsl.uab.edu/dt/2008r/king.pdf.
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Katayama, Yoshinori. « Rap signaling is crucial for the competence of IL-7 response and the development of B-lineage cells ». Kyoto University, 2009. http://hdl.handle.net/2433/126465.
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Di, Tullio Alessandro 1982. « Reprogramming of B cells into macrophages : mechanistic insights ». Doctoral thesis, Universitat Pompeu Fabra, 2012. http://hdl.handle.net/10803/85722.
Texte intégralRésumé :
Our earlier work has shown that pre-B cells can be converted into macrophages by the transcription factor C/EBPα at very high frequencies and also that a clonal pre-B cell line with an inducible form of C/EBPα can be converted into macrophage-like cells. Using these systems we have performed a systematic analysis of the questions whether during transdifferentiation the cells retrodifferentiate to a precursor cell state and whether cell cycle is required for reprogramming.
As for the first question, a transcriptome analysis of transdifferentiating cells showed that most genes are continuously up or downregulated, acquiring a macrophage phenotype within 5 days. In addition, we observed the transient reactivation of a subset of immature myeloid markers, as well as low levels of the progenitor markers Kit and Flt3 and a few lineage inappropriate genes. Importantly, we were unable to observe the re-expression of cell surface marker combinations that characterize hematopoietic stem and progenitor cells (HSPCs), including c-Kit and Flt3. This was the case even when C/EBPα was activated in pre-B cells under culture conditions that favor HSPC growth or when the transcription factor was activated in a time limited fashion.
As for the second question, using the C11-inducible pre-B cell line, time-lapse experiments showed that a subpopulation of about 8% of the pre-B cells did not divide before acquiring macrophage properties, with the majority of cells dividing once and a few percent dividing twice. In agreement with these results we found that 8% of the induced cells did not incorporate BrdU during reprogramming. Importantly, the non-dividing cell subset expressed the highest levels of C/EBPα and was the fastest in acquiring a macrophage phenotype. Inhibition of DNA synthesis by aphidicolin led to an impairment of transdifferentiation in >70% of the cells, suggesting a requirement for traversing the cell cycle. However, sorting pre-B cells into G0/G1 and G2/M fractions followed by induction showed no significant differences in the reprogramming kinetics. Finally, we showed that knocking down p53 in the inducible pre-B cells does not alter their conversion into macrophages, suggesting that an acceleration of the cell cycle has no effect.
Together, our findings show that the conversion of pre-B cells to macrophages does not involve overt retrodifferentiation and that high concentrations of C/EBPα bypass the cell cycle-dependency of immune cell transdifferentiationRecientemente, nuestro grupo ha demostrado que las células pre-B se pueden reprogramar a macrófagos mediante la sobreexpresión del factor de transcripción C/EBP, con una eficiencia elevada. Así mismo, mediante la expresión de la forma inducible de C/EBP en una línea de células pre-B (C11), éstas también se puede convertir en células similares a macrófagos. Usando este sistema hemos estudiado si durante el proceso de trans-diferenciacion las células requieren volver a un estadio de célula precursora, y si el ciclo celular es necesario para este proceso. En cuanto a la primera cuestión, el análisis del transcriptoma de células trans-diferenciadas mostró que la expresión de la mayoría de los genes están regulados durante todo el proceso bien aumentando o disminuyendo, y que adquieren el fenotipo de macrófago a los 5 días después de iniciar el proceso. Así mismo, se observó la reactivación transitoria de un grupo de genes que codifican para marcadores de células mieloides inmaduras; también cabe destacar que observamos una disminución en la expresión de los genes expresados en células progenitoras Kit y Flt3, así como de genes de linajes impropios. Es importante destacar que nunca hemos llegado a observar la expresión de combinaciones de marcadores de superficie característicos de las células madre hematopoyéticas y las células progenitoras (HSPCs), incluyendo c-Kit y Flt3, mediante el análisis por citometría de flujo. Estos resultados se reprodujeron incluso cuando C/EBP se sobreexpresó en células pre-B que fueron cultivadas en condiciones que favorecen el crecimiento de las HSPC o cuando el factor de transcripción se activó de forma limitada en el tiempo. En cuanto a la segunda pregunta, usando la línea de células inducibles pre-B C11, el análisis mediante microscopia a diferentes tiempos después de la inducción de la reprogramación mostraron que una subpoblación de aproximadamente el 8% de las células pre-B no se dividen antes de adquirir las propiedades de macrófago, mientras que la mayoría de las células se dividen sólo una vez y un pequeño porcentaje dos veces antes de que se reprogramen totalmente a macrófagos. De acuerdo con estos resultados se encontró que un 8% de las células inducidas no incorporan BrdU durante la reprogramación. Es importante destacar que el subconjunto de células que no se dividen expresan los niveles más altos de C/EBP, con lo que cabe pensar que la adquisición del fenotipo de macrófago es más rápida en estas células. La inhibición de la síntesis de ADN por afidicolina bloqueó la transdiferenciación en mas de un 70% de las células, lo que sugiere que la correcta progresión del ciclo celular es un requisito para la transdiferenciación. Sin embargo, al separar la linea de células pre-B C11 en fracciones G0/G1 y G2/M seguido de la inducción, la cinética de la reprogramación no mostró diferencias significativas. Por último, también demostramos que la reducción en la expresión de p53 en las células pre-B inducibles no altera el proceso de conversión a macrófago, lo que sugiere que la aceleración del ciclo celular no tiene ningún efecto. En conjunto, nuestros resultados muestran que la conversión de células pre-B a macrófagos no requiere retro-diferenciación y que las células con una expresión mayor de C/EBP pueden llegar a prescindir de la dependencia del ciclo celular para la trans-diferenciación de las células inmunitarias.
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Andrews, Nathan Christopher Ivanov Kostadin N. « Primary calculation of the linear heat rate generation of a BWR pin in the ATR B-11 position ». [University Park, Pa.] : Pennsylvania State University, 2010. http://honors.libraries.psu.edu/theses/approved/WorldWideIndex/EHT-238/index.html.
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Alcón, Chino Mônica Elizabeth Tatiana. « Mapeamento dos epitopos lineares de células b humanos e caninos da proteina de membrana externa h6pga4 _ricri e desenvolvimento de testes diagnósticos para rickettsia rickettsii ». reponame:Repositório Institucional da FIOCRUZ, 2015. https://www.arca.fiocruz.br/handle/icict/13989.
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Previous issue date: 2015Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
A febre maculosa (Fm) é um grave problema de saúde pública no Brasil e no mundo, apresentando a bactéria Gram- negativa, Rickettsia ricketsii, como seu agente etiológico e transmitida pelo carrapato. Esse patógeno possui em sua superfície um conjunto de proteínas de membranas externas (OMP), sendo a OMP-A, um dos antígenos imunodominantes e exclusiva do grupo da Fm. Portanto, devido a inexistência de testes sorológicos sensíveis e específicos para o diagnóstico da Fm tivemos como objetivo mapear imunologicamente a OMP H6PGA4 usando uma biblioteca de peptídeos e soro de pacientes e cães com a doença e desenvolver testes diagnosticos mais específicos. Uma biblioteca de 84 peptideos com 15 residuos de comprimento cobrindo a extensão de 429 aminoácidos da proteína H6PGA4 e contendo sequências sobrepostas de nove aminoácidos foram sintetizadas pela técnica F-moc. Os peptídeos foram ligados quimicamente a uma membrana celulósica e feitos reagir independentemente com um \201Cpool\201D de soro de pacientes com febre maculosa (SP-Fm) e de soro de cães com febre maculosa (SC-Fm). Oito epitopos IgG foram identificados pelo soro de pacientes e três epitopos IgG pelo SC-Fm Todos os peptídeos foram análisados individualmente por ELISA-peptideo contra um painel de SP-Fm e SC-Fm. A análise realizada através da curva ROC indicou que os peptídeos E4 e E5 foram os mais imunogênicos com SP-Fm (especificidade de 90% e sensibilidade de 94%). Diferentemente os peptídeos E7 e E9 foram os que apresentaram especificidade e sensibilidade (p<0,05) acima de 90% e 94% com SC-Fm, repectivamente. Alternativamente, foi desenvolvido um imunosensor para diagnóstico da doença humana, baseado no peptídeo OMP3 e análise por voltametria cíclica. A acurácia foi demonstrada pela análise de 20 ciclos de varredura e a especificidade contra soros de indivíduos saudáveis. O chip imunosensor-peptideo foi reprodutível com um coeficiente de variação \226410,1% para SP-Fm. A sensibilidade de diluição do soro foi de até 1: 100
Rocky Mountain spotted fever (Fm) is a serious public health problem in Brazil and in the world, presenting the Gram- negative bacteria, Rickettsia ricketsii, as its etiologic agent and transmitted by the bite of an infected tick. This pathogen has on its surface a set of outer membrane proteins (OMP), being the OMP-A one of the immunodominant and exclusive antigen of the Fm group. Therefore, due to the lack of sensitivity and specificity of the serologic tests for the diagnosis of Fm our objective was to map the immunologically OMP H6PGA4_ RICRI using a peptide library and serum of patients and dogs with the disease and develop more specific diagnostic tests. A library of 84 peptides with 15 residues in length covering the extension of 429 amino acids of the H6PGA4 protein and containing overlapping sequences of nine amino acids were synthesized by F-moc technique. The peptides were chemically bound to a cellulose membrane and reacted independently with serum from patients (n=5) with spotted fever (SP-Fm) and serum from dogs (n=5) with spotted fever (SC-Fm). Eight IgG epitopes were identified by the SP-Fm and three by the SC-Fm. All peptides were individually analyzed by ELISA-peptide using a panel of SP-Fm and SC-Fm The analysis of ROC curve indicated that the E4 and E5 peptides were the most immunogenic with a specificity of 90% and sensitivity of 94% for the SP-Fm. Unlike the E7 and E9 peptides were the most reactive for SC-Fm with a specificity of 90% and sensitivity of 94% (p <0.05). Alternatively, it was developed a cyclic voltametry E3 based immunesensor for the diagnosis of the human disease. The accuracy was demonstrated by the analysis of 20 cycles of scanning and the specificity against sera of healthy individuals. The peptide-imunosensor chip was reproducible with a coefficient of variation \2264 10,1% for SP-Fm. The sensitivity of serum dilution was 1: 100
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Zong, Weikai. « Amplitude and frequency modulations of oscillation modes in hot B subdwarf and white dwarf stars from Kepler photometry ». Thesis, Toulouse 3, 2016. http://www.theses.fr/2016TOU30306/document.
Texte intégralRésumé :
Les interactions non linéaires entre modes de pulsation, induisant des modulations d'amplitude et de fréquence, sont difficiles à mettre en évidence avec les télescopes au sol en raison des temps caractéristiques en jeu, de l'ordre de la semaine, du mois, ou même de l'année. L'avènement des télescopes spatiaux comme KEPLER (opéré par la NASA) a considérablement changé la donne en apportant de nouvelles données pour ce domaine de recherche. Dans cette thèse, nous analysons les données photométriques obtenues avec KEPLER pour 24 étoiles compactes pulsantes, incluant 18 étoiles sous-naines de type B (sdB) et 6 naines blanches. Nous établissons que les modulations d'amplitude et de fréquence des modes d'oscillation sont un phénomène courant dans ces étoiles. Nous étudions en particulier deux étoiles : KIC 0862602, une naine blanche pulsante de type DB, et KIC 10139564, une étoile sdB variable à courtes périodes. KIC 0862602 et KIC 10139564 ont été observées sans interruption par KEPLER en cadence rapide pendant deux années pour la première et plus de trois ans pour la seconde. En analysant en détail ces données photométriques de très haute précision, nous mettons en évidence différents types de comportements affectant les composantes de triplets induits par la rotation stellaire. Les fréquences et amplitudes de ces modes montrent des variations soi périodiques soi irrégulières, ou demeurent constantes. Ces comportements peuvent être connectés à ceux prédits par les équations d'amplitude dans le cas de couplages non linéaires résonants entre modes, en particulier pour les temps caractéristiques des modulations. De plus, nous montrons que les modes en résonance constituant les triplets peuvent également interagir avec des modes extérieurs par le biais d'autres formes de résonances telle que la résonance à trois modes v 0 ~ v 1 + v 2 , une situation qui n'est pas prise en compte dans le cadre théorique existant. Ces études apportent pour la première fois une preuve claire de l'existence de mécanismes de couplages non linéaires entre modes d'oscillations dans les pulsateurs compacts. Cette découverte résonne comme un avertissement pour les projets visant à mesurer les taux de changement des périodes dus à l'évolution dans les étoiles compactes en général. Les modulations de fréquence d'origine non linéaire peuvent potentiellement empêcher toute mesure fiable de ces taux, à moins de corriger ces effets auparavant. Les modulations observées caractérisées dans cette thèse apportent un regard nouveau sur "l'astérosismologie non linéaire" et appellent à revisiter les méthodes d'analyse des courbes de lumière pour en extraire les modes d'amplitude et de fréquence variables. Dans un futur proche, nous anticipons davantage d'attention portée à ces phénomènes non inéaires dans différents types d'étoiles pulsantes observées depuis l'espace, ainsi qu'un regain d'intérêt pour la théorie non linéaire des oscillations stellaires en généralNonlinear mode interactions, inducing amplitude and frequency modulations, are difficult to observe from ground-based telescopes as these typical timescales of the modulations are of the order of weeks, months, or even years. The launch of space telescopes such as Kepler (operated by NASA) has tremendously changed the situation by providing new data for this research field. In this thesis, we analyze the Kepler photometric data observed for 24 compact pulsators, including 18 hot B subdwarf (sdB) stars and six white dwarf stars. We find that it is a common phenomenon that oscillation modes in these pulsating stars show amplitude and/or frequency variations. We focus in particular on two stars, KIC 08626021, a DB white dwarf, and KIC 10139564, a short period sdB star. KIC 08626021 and KIC 10139564 have been monitored by Kepler in short-cadence mode for nearly two years and more than three years without interruption, respectively. By analyzing in depth these high-quality photometric data, we find that the modes within triplets induced by rotation clearly reveal different types of behaviors : their frequency and amplitude may exhibit either periodic or irregular modulations, or remain constant. These various behaviors can be linked to those predicted within the amplitude equation formalism in the case of the nonlinear resonant mode coupling mechanism, particularly for the modulation timescales. Furthermore, we find that the triplet resonance modes can also interact with outside modes through other types of resonances such as the three-mode resonance v 0 ~ v 1 + v 2 , which is not considered within the current nonlinear theoretical frameworks. These findings constitute the first clear evidence of nonlinear resonant mode couplings occurring in compact pulsators. This should resonate as a warning to projects aiming at measuring the evolutionary change rate of pulsation periods in compact stars in general. Nonlinear modulations of the frequencies can potentially jeopardize any attempt to measure reliably such rates, unless they can be corrected beforehand. The observed modulations characterized in this thesis provide new insights to "nonlinear asteroseismology" and call for new methods to process the signals of variable modes from the observed light curves. We foresee that increasing attention will focus on these nonlinear phenomena in various types of pulsating stars observed from space in the near future, thus reviving interest in the nonlinear oscillation theory in general
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Verkoczy, Laurent Karl. « Molecular cloning and characterization of hBRAG and other genes as potential regulators of RAG1 in the B cell lineage ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape4/PQDD_0023/NQ50054.pdf.
Texte intégral
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Schroyer, April L. « The Regulation of Mixed Lineage Kinase 3 by Extracellular Signal-Regulated Kinases 1 and 2 and Stress Stimuli in Colorectal and Ovarian Cancer Cells ». University of Toledo / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1513345931716064.
Texte intégral
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Pruna, Giovanni Marco. « Phenomenology of the minimal B-L Model : the Higgs sector at the Large Hadron Collider and future linear colliders ». Thesis, University of Southampton, 2011. https://eprints.soton.ac.uk/199955/.
Texte intégralRésumé :
This thesis is devoted to the study of the phenomenology of the Higgs sector of the minimal B −L extension of the Standard Model at present and future colliders. Firstly, the motivations that call for the minimal B −L extension are summarised. In addition, the model is analysed in its salient parts. Moreover, a detailed review of the phenomenological allowed Higgs sector parameter space is given. Finally, a complete survey of the distinctive Higgs boson signatures of the model at both the Large Hadron Collider and the future linear colliders is presented
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Aurora, Federico. « Chronological and geographical annotations in DAMOS : database of Mycenaean at Oslo ». Epigraphy Edit-a-thon : editing chronological and geographic data in ancient inscriptions ; April 20-22, 2016 / edited by Monica Berti. Leipzig, 2016. Beitrag 1, 2016. https://ul.qucosa.de/id/qucosa%3A15464.
Texte intégralRésumé :
DAMOS is an online annotated database (MySql) of all published texts of Mycenaean, the earliest attested Greek dialect. The texts are annotated for epigraphical and linguistic features (morphology, syntax, semantics) and provided with a rich set of metadata, which also include chronological and geographical data. Genre (administrative accounts) and physical features (brevity and often fragmentary state) of the Mycenaean texts, and especially their script (Linear B), not well suited for rendering the
Greek language, pose challenges to the interpretation of the texts, which often result in multiple possible values of the data at all levels – epigraphical, linguistic, metadata. These may often be organized in competing sets of values, which form coherent different overarching hypotheses on e.g. the grammar of the language or the dating of an archive. These competing values need, thus, to be stored and meaningfully organized in the database. The presentation focuses on how chronological and geographical data (both about the texts and contained in the texts) and their often multiple possible values are dealt with in the arrangement of the database structure of DAMOS.