Bibliographies thématiques / LED-induced fluorescence

Littérature scientifique sur le sujet « LED-induced fluorescence »

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Publié le 25 mai 2024

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Articles de revues sur le sujet "LED-induced fluorescence"

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Geng, Xuhui, et Yafeng Guan. « Research highlight on CJAC—LED induced fluorescence detector ». Chinese Journal of Analytical Chemistry 50, no 5 (mai 2022) : 100084. http://dx.doi.org/10.1016/j.cjac.2022.100084.

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Gu, Wenwen, Jie Huang et Weimin Tan. « LED induced fluorescence detector integrated in microfluidic cell chip ». International Journal of Nanotechnology 12, no 10/11/12 (2015) : 742. http://dx.doi.org/10.1504/ijnt.2015.071785.

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Qi, Xiaoguang, Xianglong Hao, Muzi Zhang, Lili Jiang, Wenyue Gao et Chi Wu. « Extensible LED-Induced Integrated Fluorescence Detection Module for Quantitative Analysis of Lucigenin Concentration ». Photonics 10, no 4 (1 avril 2023) : 392. http://dx.doi.org/10.3390/photonics10040392.

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We developed an extensible LED-induced fluorescence detection module with a highly integrated and ultra-compact structure. A target-oriented design methodology was used to demonstrate the module’s optimal design. Lucigenin solution was used as a test sample in evaluation trials to demonstrate the module’s quantitative fluorescence detection capability. Results showed that the integrated module has an outstanding linear response in the range of 0–1 μmol·L−1, with sensitivity and limit of detection (LOD) of 0.1692 V/μmol·L−1 and 0.03 μmol·L−1, respectively. Statistical analyses showed that our integrated module has extremely high repeatability and accuracy, i.e., the values of Pearson’s correlation coefficient and root-mean-square error exceeded 0.9995 and 1.8‰, respectively. More importantly, the integrated module possesses favorable extensibility and can realize on-demand rapid fluorescence-signal detection of other targets using appropriate parameter combinations. This module offers new opportunities for reliable, cost-effective and easy-to-use fluorescence-signal detection, especially in resource-constrained fluorescence detection applications.
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Mukunda, Darshan Chikkanayakanahalli, Jackson Rodrigues, Vijay Kumar Joshi, Chandavalli Ramappa Raghushaker et Krishna Kishore Mahato. « A comprehensive review on LED-induced fluorescence in diagnostic pathology ». Biosensors and Bioelectronics 209 (août 2022) : 114230. http://dx.doi.org/10.1016/j.bios.2022.114230.

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GU Wen-wen, 顾雯雯. « LED induced transmitted fluorescence detector integrated in microfluidic cell chip ». Optics and Precision Engineering 22, no 8 (2014) : 2159–65. http://dx.doi.org/10.3788/ope.20142208.2159.

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Mustafic, Adnan, Changying Li et Mark Haidekker. « Blue and UV LED-induced fluorescence in cotton foreign matter ». Journal of Biological Engineering 8, no 1 (2014) : 29. http://dx.doi.org/10.1186/1754-1611-8-29.

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Dong, Yongjiang, Xuan Liu, Liang Mei, Chao Feng, Chunsheng Yan et Sailing He. « LED-induced fluorescence system for tea classification and quality assessment ». Journal of Food Engineering 137 (septembre 2014) : 95–100. http://dx.doi.org/10.1016/j.jfoodeng.2014.03.027.

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Gao, Fei, Yongjiang Dong, Weimin Xiao, Bin Yin, Chunsheng Yan et Sailing He. « LED-induced fluorescence spectroscopy technique for apple freshness and quality detection ». Postharvest Biology and Technology 119 (septembre 2016) : 27–32. http://dx.doi.org/10.1016/j.postharvbio.2016.04.020.

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Rodat-Boutonnet, Audrey, Pierre Naccache, Arnaud Morin, Jacques Fabre, Bernard Feurer et François Couderc. « A comparative study of LED-induced fluorescence and laser-induced fluorescence in SDS-CGE : Application to the analysis of antibodies ». ELECTROPHORESIS 33, no 12 (28 juin 2012) : 1709–14. http://dx.doi.org/10.1002/elps.201200132.

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Grochocki, Wojciech, Magdalena Buszewska-Forajta, Szymon Macioszek et Michał J. Markuszewski. « Determination of Urinary Pterins by Capillary Electrophoresis Coupled with LED-Induced Fluorescence Detector ». Molecules 24, no 6 (24 mars 2019) : 1166. http://dx.doi.org/10.3390/molecules24061166.

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Urinary pterins have been found as potential biomarkers in many pathophysiological conditions including inflammation, viral infections, and cancer. However, pterins determination in biological samples is difficult due to their degradation under exposure to air, light, and heat. Besides, they occur at shallow concentration levels, and thus, standard UV detectors cannot be used without additional sample preconcentration. On the other hand, ultra-sensitive laser-induced fluorescence (LIF) detection can be used since pterins exhibit native fluorescence. The main factor that limits an everyday use of LIF detectors is its high price. Here, an alternative detector, i.e., light-emitted diode induced fluorescence (LEDIF) detector, was evaluated for the determination of pterins in urine samples after capillary electrophoresis (CE) separation. An optimized method was validated in terms of linearity range, limit of detection (LOD), limit of quantification (LOQ), intra- and interday precision and accuracy, sample stability in the autosampler, and sample stability during the freezing/thawing cycle. The obtained LOD (0.1 µM) and LOQ (0.3 µM) values were three-order of magnitude lower compared to UV detector, and two orders of magnitude higher compared to previously reported house-built LIF detector. The applicability of the validated method was demonstrated in the analysis of urine samples from healthy individuals and cancer patients.
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Thèses sur le sujet "LED-induced fluorescence"

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Stjernlöf, Anna. « Portable capillary electrophoresis system with LED-absorbance photometric and LED-induced fluorescence detection : Design, characterisation and testing ». Thesis, Karlstad University, Division for Chemistry, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-1364.

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Capillary electrophoresis (CE) has a wide range of applications in the field of analytical chemistry. In general the most expensive part in a CE system is the detector due to the fact that the detector must have a high sensitivity for small detection volumes and low concentrations. Building portable instruments is one way to make the instruments cheaper and has the advantage that they can be used virtually everywhere. However, downscaling of CE instruments puts some extra demands on the detector. This report describes the design and building of two homemade light-emitting diode (LED) based detectors; a LEDabsorbance photometric detector (LED-AP) and a LED-induced fluorescence (LED-IF) detector. The main goal was to install them inside a portable CE and make a simple separation. The performance of the two detectors had to be evaluated before the main goal could be achieved. p-Nitrophenol was used to create a sensitivity graph for the LED-AP detector, calculating the upper linearity to 5.6 mM when the sensitivity had dropped 10 % caused by non-linearity. The sensitivity graph also showed that the detector had an effective pathlength of 74.2 µm and a stray light of 4.5 % for a 75 µm i.d fused-silica capillary. The LED-IF detector was evaluated by determining the limit of detection (LOD) for fluorescein, at a signal to noise ratio of 3. The LOD was 0.72 µM ± 0.01 µM when immersion oil was used to limit the light scattering from the optic fibres in to the capillary and 0.58 µM ±0.02 µM when silicone oil was used. Without doing any improvements only the LED-AP detector could be used in the portable CE. As a common application area for portable CE instruments is environmental analysis, indirect detection using p-nitrophenol as a probe for separating anions was done to test the system. All analytes were eluted in less than 4 minutes.

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Sharikova, Anna V. « UV Laser and LED Induced Fluorescence Spectroscopy for Detection of Trace Amounts of Organics in Drinking Water and Water Sources ». Scholar Commons, 2009. https://scholarcommons.usf.edu/etd/15.

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A UV Laser Induced Fluorescence (LIF) system, previously developed in our laboratory, was modified and used for a series of applications related to the development and optimization of UV LIF spectroscopic measurements of trace contaminants in drinking water and other water sources. Fluorescence spectra of a number of water samples were studied, including those related to the reverse osmosis water treatment and membrane fouling, domestic and international drinking water, industrial toxins, bacterial spores, as well as several fluorescence standards. Of importance was that the long term detection of the trace level of Dissolved Organic Compounds (DOC) was measured, for the first time to our knowledge, over a one week period and with a time resolution of 2.5 minutes. A comparison of LIF emission using both 266 nm and 355 nm excitation was also made for the first time. Such real-time and continuous measurements are important for future water treatment control. The LIF system was modified to accommodate UV Light Emitting Diodes (LED) as alternative excitation sources, and tested for the detection of trace organic species in water. In addition, a compact system using LED excitation and a spectrometer was xviii developed and underwent initial testing. The original LIF system had two laser sources, 266 nm and 355 nm. The additional sources incorporated in the system were UV LEDs emitting at 265 nm, 300 nm, 335 nm and 355 nm. The LED spectral emission was studied in detail, in terms of spectral variability and power output. It was found that all LEDs had some emission in the visible spectrum, and an optical filter was used to remove it. The signal-to-noise ratio for the LED-based systems was determined and compared with that of the LIF system. The fluorescent signal of the LED-based system was smaller by 1 to 2 orders of magnitude, despite the fact that the LED pulse energy was 2 to 3 orders of magnitude less than the laser's. As such, the fluorescent signal from the LED was greater than expected. Therefore, a UV LED may be a compact and much cheaper optical source for future water measurement instruments.
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Loayza, Loza Hildo. « Suivi expérimental du rendement de fluorescence des couverts végétaux par des techniques actives et passives. Application à la détection du stress hydrique ». Electronic Thesis or Diss., Sorbonne université, 2022. http://www.theses.fr/2022SORUS465.

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La fluorescence de la chlorophylle (ChlF) est directement liée au processus photosynthétique. Cependant, au niveau de la canopée, ce lien physiologique entre la fluorescence et la photosynthèse peut être brouillé par les changements structurels de la végétation et les interactions entre la lumière du soleil et la structure 3D de la canopée. De plus, une grande partie de nos connaissances sur la relation entre la fluorescence et l'état physiologique des plantes provient d'études au niveau des feuilles réalisées dans des conditions de laboratoire. La signification physiologique de la ChlF au niveau de la canopée et dans des conditions naturelles est toujours un sujet de recherche majeur. Ce projet doctoral avait pour objectifs : 1. Etude du rendement de fluorescence de la chlorophylle au niveau de la canopée: nous décrivons un nouvel instrument, Ledflex, qui est un micro-LIDAR dédié à effectuer des mesures continues du rendement de fluorescence de la végétation. Ledflex a été appliqué avec succès dans des conditions de plein soleil pour établir la signature du stress hydrique sur la canopée du pois (Pisum Sativum). Dans des conditions bien irriguées, le cycle diurne du rendement de fluorescence observé (Fs) présente une forme en M avec un minimum (Fmin) vers midi supérieur au niveau observé à l’obscurité (Fo). Après plusieurs jours sans irrigation, Fs diminue et Fmin
The chlorophyll fluorescence (ChlF) is directly related to the photosynthetic process. However, at canopy level this physiological link between fluorescence and photosynthesis may be blurred by structural vegetation changes and geometrical effects linked to interactions between sunlight and the three-dimensional structure of the canopy. Furthermore, much of our knowledge about the relationship between fluorescence and the physiological status of plants come from leaf level studies carried out under laboratory conditions. The physiological significance of ChlF at canopy level and under natural conditions is still a major subject of research and a source of uncertainties in the interpretation of SIF. This doctoral project aims were: 1. To study chlorophyll fluorescence yield at canopy level: we describe a new instrument, Ledflex, which is a micro-LIDAR dedicated to perform continuous measurements of vegetation fluorescence yield. Ledflex has been successfully applied under full sunlight conditions to establish the signature of water-stress on a pea (Pisum Sativum) canopy. Under well-watered conditions the Fs diurnal cycle present an M shape with a minimum (Fmin) at noon which is higher than the fluorescence level observed at predawn (Fo). After several days withholding watering, Fs decreases and Fmin
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Cheng, Mao-Cheng, et 鄭茂成. « Determination of Metabolites of Ethylene Glycol Ethers in Urine by Capillary Electrophoresis with Indirect LED Induced Fluorescence Detection ». Thesis, 2007. http://ndltd.ncl.edu.tw/handle/89397123452504100788.

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碩士
國立嘉義大學
應用化學系研究所
95
Ethylene glycol monomethyl ether (EGME), ethylene glycol monoethyl ether (EGEE) and their acetates are broadly used as solvent in industries. Due to their satisfactory chemical and physical properties, they have been used in paints, lacquer, inks and semiconductor industry. They enter human bodies by ingestion, inhalation or dermal penetration and are metabolized to methoxyacetic acid (MAA) and ethoxyacetic acid (EAA) by alcohol dehydrogenase and aldehyde dehydrogenase. The metabolites cause testicular atrophy, teratogenicity, hematological toxicity and carcinogenicity in human. The aim of our study is to provide a method based on capillary electrophoresis with indirect LED-induced fluorescence detection to determine MAA and EAA in urine. The background electrolyte providing optimal separation and maximum signal-to-noise ratio was consisted of 4 mM tetraborate buffer, 500 μM fluorescein and 10% acetonitrile at pH 9.20. The separation voltage was 15kV. The dynamic ranges of MAA and EAA were 5.76 - 345.45 and 1.07 -213.40 μg/ml with R-square of 0.9985 and 0.9992, respectively. The limits of detection for MAA and EAA were 0.83 and 0.46 μg/ml, respectively. The analysis of the urinary samples spiked with MAA and EAA were finished in 10 min, and the recovery of MAA was 95.4 - 98.8 % and that of EAA was 97.7 - 101.4 %. It showed that a simple, fast, and less expensive method for analysis of metabolites of ethylene glycol ethers in urine was developed.
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張喦升. « UV light emitting diode (LED)-induced fluorescence detection combined with online sample concentration techniques for use in capillary electrophoresis ». Thesis, 2005. http://ndltd.ncl.edu.tw/handle/81881177691939412746.

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碩士
國立臺灣師範大學
化學系
93
Abstract The application of an ultraviolet (UV) light emitting diode (LED) to on-line sample concentration/fluorescence detection in capillary electrophoresis (CE) is described. The utility of UV-LED (peak emission wavelength at 380 nm, ~ 2 mW) for fluorescence detection is demonstrated by examining a naturally fluorescent (riboflavin) and a non-fluorescent compound (tryptophan), respectively. The detection limit for riboflavin was determined to be 0.2 ppm by the normal MEKC mode and this was improved to 3 ~ 7 ppb when a dynamic pH-junction techniques were applied. On the other hand, the detection limit of the tryptophan derivative was determined to be 1.5 ppm using the MEKC mode and this was improved to 3 ppb when the sweeping-MEKC mode was applied. In an analysis of an actual sample, the concentrations of riboflavin and tryptophan in beer and urine/milk samples were determined, respectively.
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Peng, Wen-Chung, et 彭文忠. « Design of Programmable Multi-band Light Emitting Diode (LED) Induced Fluorescent Light Source System ». Thesis, 2019. http://ndltd.ncl.edu.tw/handle/2q8kzs.

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Mandal, Kuheli. « Fluorescent Bioimaging Probe from Aggregation Induced Emission Active Molecule ». Thesis, 2019. http://hdl.handle.net/10821/8341.

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Chapitres de livres sur le sujet "LED-induced fluorescence"

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Javad Ahmadi-Lahijani, Mohammad, et Saeed Moori. « Photosynthetic Response and Adaptation of Plants in Perspective of Global Climate Change ». Dans Abiotic Stress in Plants - Adaptations to Climate Change [Working Title]. IntechOpen, 2023. http://dx.doi.org/10.5772/intechopen.109544.

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The intense agricultural and human being activities, especially after the industrialization era, have increased the CO2 concentration, which led to changes in the global climate. Climate change and its consequences, that is, elevated CO2, water stress, and extreme temperatures, have induced many biotic and abiotic stresses and have caused alterations in plant physiology, leading to a reduced photosynthetic capacity of plants. Photosynthesis is the most crucial biochemical process in plants that determines the final dry matter production and productivity of plants. The efficiency and status of the photosynthetic apparatus can be measured by the measurement of chlorophyll fluorescence. Measurements of chlorophyll fluorescence are easy, non-destructive, and quick, and it reflects changes in the general bioenergy status of a plant. Studies have indicated that abiotic stresses emerging from climate changes cause changes in the biological processes of plants and damage the internal structure of photosynthesis and control of the cellular process. Chlorophyll fluorescence, meanwhile, is an effective parameter and an indicator of photosynthetic status and its mechanisms under stressful conditions. Therefore, the photosynthetic changes and adaptation and the role of chlorophyll fluorescence in determining its status under climate change are discussed in this chapter.
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Actes de conférences sur le sujet "LED-induced fluorescence"

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Liu, Xuan, Chao Feng et Chunsheng Yan. « Classification of tea using LED-induced fluorescence ». Dans 2013 22nd Wireless and Optical Communication Conference (WOCC 2013). IEEE, 2013. http://dx.doi.org/10.1109/wocc.2013.6676446.

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Babin, François, Marc Levesque, Louis Saint-Laurent, Henrique Weber, Simon Turbide, E. Virginia Foot et Camilla V. Robinson. « Biosensing across wide areas using LED induced fluorescence ». Dans Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXI, sous la direction de Jason A. Guicheteau et Chris R. Howle. SPIE, 2020. http://dx.doi.org/10.1117/12.2560078.

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Wanrong Ding, Fei Gao et Chunsheng Yan. « LED-induced fluorescence spectroscopy technique for milk freshness detection ». Dans 2016 15th International Conference on Optical Communications and Networks (ICOCN). IEEE, 2016. http://dx.doi.org/10.1109/icocn.2016.7875649.

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Zhong, Weijia, Yongjiang Dong, Xuan Liu, Hongze Lin, Liang Mei et Chunsheng Yan. « Classification evaluation of tobaccos using LED-induced fluorescence spectroscopy ». Dans SPIE OPTO, sous la direction de Klaus P. Streubel, Heonsu Jeon, Li-Wei Tu et Martin Strassburg. SPIE, 2014. http://dx.doi.org/10.1117/12.2039367.

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Allison, Stephen W., David L. Beshears, Michael R. Cates, M. Paranthaman et George T. Gilles. « LED-induced fluorescence diagnostics for turbine and combustion engine thermometry ». Dans International Symposium on Optical Science and Technology, sous la direction de Carolyn R. Mercer, Soyoung S. Cha et Gongxin Shen. SPIE, 2001. http://dx.doi.org/10.1117/12.449386.

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Rostampour, V., et M. J. Lynch. « Quantitative techniques to discriminate petroleum oils using LED-induced fluorescence ». Dans WATER POLLUTION 2006. Southampton, UK : WIT Press, 2006. http://dx.doi.org/10.2495/wp060261.

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Zhong, Keke, Zhangwei Chen, Jing Huang, He Mao et Kewei Hu. « A LED-induced confocal fluorescence detection system for quantitative PCR instruments ». Dans 2011 4th International Conference on Biomedical Engineering and Informatics (BMEI). IEEE, 2011. http://dx.doi.org/10.1109/bmei.2011.6098382.

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Zhiguang Zhang, Xiaoqiong Li, Xuefei Lv, Xiaoming Hu, L. Geng et Yulin Deng. « A signal acquisition and data processing system for LED induced fluorescence detector ». Dans 2013 ICME International Conference on Complex Medical Engineering (CME 2013). IEEE, 2013. http://dx.doi.org/10.1109/iccme.2013.6548246.

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Le Jing, Li Yun, Hua Dengxin, Tan Linqiu et Cao Ning. « Research on method of LED-induced chlorophyll fluorescence spectrum and image information acquisition ». Dans Instruments (ICEMI). IEEE, 2011. http://dx.doi.org/10.1109/icemi.2011.6037913.

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Sharikova, Anna V., et Dennis K. Killinger. « Laser- and UV-LED-induced fluorescence detection of dissolved organic compounds in water ». Dans SPIE Defense, Security, and Sensing, sous la direction de Edward M. Carapezza. SPIE, 2010. http://dx.doi.org/10.1117/12.850342.

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Rapports d'organisations sur le sujet "LED-induced fluorescence"

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Avni, Adi, et Gitta L. Coaker. Proteomic investigation of a tomato receptor like protein recognizing fungal pathogens. United States Department of Agriculture, janvier 2015. http://dx.doi.org/10.32747/2015.7600030.bard.

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Maximizing food production with minimal negative effects on the environment remains a long-term challenge for sustainable food production. Microbial pathogens cause devastating diseases, minimizing crop losses by controlling plant diseases can contribute significantly to this goal. All plants possess an innate immune system that is activated after recognition of microbial-derived molecules. The fungal protein Eix induces defense responses in tomato and tobacco. Plants recognize Eix through a leucine-rich-repeat receptor- like-protein (LRR-RLP) termed LeEix. Despite the knowledge obtained from studies on tomato, relatively little is known about signaling initiated by RLP-type immune receptors. The focus of this grant proposal is to generate a foundational understanding of how the tomato xylanase receptor LeEix2 signals to confer defense responses. LeEix2 recognition results in pattern triggered immunity (PTI). The grant has two main aims: (1) Isolate the LeEix2 protein complex in an active and resting state; (2) Examine the biological function of the identified proteins in relation to LeEix2 signaling upon perception of the xylanase elicitor Eix. We used two separate approaches to isolate receptor interacting proteins. Transgenic tomato plants expressing LeEix2 fused to the GFP tag were used to identify complex components at a resting and activated state. LeEix2 complexes were purified by mass spectrometry and associated proteins identified by mass spectrometry. We identified novel proteins that interact with LeEix receptor by proteomics analysis. We identified two dynamin related proteins (DRPs), a coiled coil – nucleotide binding site leucine rich repeat (SlNRC4a) protein. In the second approach we used the split ubiquitin yeast two hybrid (Y2H) screen system to identified receptor-like protein kinase At5g24010-like (SlRLK-like) (Solyc01g094920.2.1) as an interactor of LeEIX2. We examined the role of SlNRC4a in plant immunity. Co-immunoprecipitation demonstrates that SlNRC4a is able to associate with different PRRs. Physiological assays with specific elicitors revealed that SlNRC4a generally alters PRR-mediated responses. SlNRC4a overexpression enhances defense responses while silencing SlNRC4 reduces plant immunity. We propose that SlNRC4a acts as a non-canonical positive regulator of immunity mediated by diverse PRRs. Thus, SlNRC4a could link both intracellular and extracellular immune perception. SlDRP2A localizes at the plasma membrane. Overexpression of SlDRP2A increases the sub-population of LeEIX2 inVHAa1 endosomes, and enhances LeEIX2- and FLS2-mediated defense. The effect of SlDRP2A on induction of plant immunity highlights the importance of endomembrane components and endocytosis in signal propagation during plant immune . The interaction of LeEIX2 with SlRLK-like was verified using co- immunoprecipitation and a bimolecular fluorescence complementation assay. The defence responses induced by EIX were markedly reduced when SlRLK-like was over-expressed, and mutation of slrlk-likeusing CRISPR/Cas9 increased EIX- induced ethylene production and SlACSgene expression in tomato. Co-expression of SlRLK-like with different RLPs and RLKs led to their degradation, apparently through an endoplasmic reticulum-associated degradation process. We provided new knowledge and expertise relevant to expression of specific be exploited to enhance immunity in crops enabling the development of novel environmentally friendly disease control strategies.
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