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Littérature scientifique sur le sujet « LdtF »

Auteur : Grafiati

Publié le 9 mars 2023

Mis à jour le 10 mars 2023

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Articles de revues sur le sujet "LdtF"

Bahadur, Raj, Pavan Kumar Chodisetti et Manjula Reddy. « Cleavage of Braun’s lipoprotein Lpp from the bacterial peptidoglycan by a paralog of l,d-transpeptidases, LdtF ». Proceedings of the National Academy of Sciences 118, n^o 19 (3 mai 2021) : e2101989118. http://dx.doi.org/10.1073/pnas.2101989118.

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The gram‐negative bacterial cell envelope is made up of an outer membrane (OM), an inner membrane (IM) that surrounds the cytoplasm, and a periplasmic space between the two membranes containing peptidoglycan (PG or murein). PG is an elastic polymer that forms a mesh-like sacculus around the IM, protecting cells from turgor and environmental stress conditions. In several bacteria, including Escherichia coli, the OM is tethered to PG by an abundant OM lipoprotein, Lpp (or Braun’s lipoprotein), that functions to maintain the structural and functional integrity of the cell envelope. Since its discovery, Lpp has been studied extensively, and although l,d-transpeptidases, the enzymes that catalyze the formation of PG−Lpp linkages, have been earlier identified, it is not known how these linkages are modulated. Here, using genetic and biochemical approaches, we show that LdtF (formerly yafK), a newly identified paralog of l,d-transpeptidases in E. coli, is a murein hydrolytic enzyme that catalyzes cleavage of Lpp from the PG sacculus. LdtF also exhibits glycine-specific carboxypeptidase activity on muropeptides containing a terminal glycine residue. LdtF was earlier presumed to be an l,d-transpeptidase; however, our results show that it is indeed an l,d-endopeptidase that hydrolyzes the products generated by the l,d-transpeptidases. To summarize, this study describes the discovery of a murein endopeptidase with a hitherto unknown catalytic specificity that removes the PG−Lpp cross-links, suggesting a role for LdtF in the regulation of PG–OM linkages to maintain the structural integrity of the bacterial cell envelope.

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Coleman, Steven M. « PAGES Lake Drilling Task Force (LDTF) ». PAGES news 7, n^o 1 (mars 1999) : 13–14. http://dx.doi.org/10.22498/pages.7.1.13.

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Casadei, F., C. Delzano, G. Magonette, J. P. Halleux et G. Verzeletti. « Dynamic testing of large AISI-316L steel specimens behaviour using LDTF ». Nuclear Engineering and Design 102, n^o 3 (juillet 1987) : 463–74. http://dx.doi.org/10.1016/0029-5493(87)90191-9.

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Jones, P. M., F. Casadei et J. P. Halleux. « Progress in numerical modelling of dynamic specimen behaviour in the LDTF ». Nuclear Engineering and Design 112 (mars 1989) : 51–64. http://dx.doi.org/10.1016/0029-5493(89)90145-3.

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Delzano, C., E. Gutierrez, P. M. Jones et G. Verzeletti. « Recent developments and results of LDTF tests on steel and concrete specimens ». Nuclear Engineering and Design 112 (mars 1989) : 65–80. http://dx.doi.org/10.1016/0029-5493(89)90146-5.

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Garnsworthy, P. C. « The interaction between dietary fibre level and protein degradability in dairy cows ». Animal Science 48, n^o 2 (avril 1989) : 271–81. http://dx.doi.org/10.1017/s0003356100040277.

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ABSTRACTA trial was performed to investigate the interaction between energy and protein source for cows in negative energy balance. Six cows were assigned to each of four types of concentrate (metabolizable energy (ME) 12 MJ/kg dry matter (DM), crude protein (CP) 180 g/kg DM) with low or high fibre contents (LF or HF) and protein degradability (LD or HD). For groups HDLF, LDLF, HDHF and LDHF respectively, acid-detergent fibre contents (g/kg diet DM) were 69·2, 66·1, 117·5 and 113·3; protein degradability values were determined as 0·78, 0·61, 0·72 and 0·66. Low-fibre diets were given at the rate of 11 kg/day concentrates with 6 kg/day hay (ME 8 MJ/kg DM, CP 84·5 g/kg DM) and high-fibre diets at the rate of 10 kg/day concentrates with 7·5 kg/day hay from weeks 4 to 13 of lactation. Cows were given a standard diet over the first 3 weeks of lactation and performance in week 3 was used as a covariate.Milk yield was not affected by treatment but the butterfat content of milk from cows given the high-fibre diets (44·9 g/kg) was higher than the low-fibre diets (36·4 g/kg; P < 0·01), which resulted in significant differences in fat-corrected milk yield (HDHF: 25·1, LDHF: 26·2, HDLF: 22·7, LDLF: 21·5, s.e.d. 1·5 kg/day). ME balance was lower for the LDHF group (−4·1 MJ/day) than for groups LDLF and HDLF (-15·4 and -16·8 MJ/day; P < 0·05) but was not significantly different from the HDHF group (-29·4 MJ/day). Undegradable protein (UDP) balances were 53·4, 93·8, -21·2 and 193·8 (s.e.d. 45, P < 005) g/day for groups HDLF, LDLF, HDHF and LDHF respectively. Differences from zero in ME and UDP balance were accounted for in all groups except LDHF by changes in live weight plus a systematic correction of approximately 10 MJ/day. It is considered that the cows given high-fibre diets responded to high UDP by increasing fat-corrected milk production but mobilization of body fat to support this increase was accompanied by retention of protein with associated water, resulting in very little change in live weight. It is also probable that the high UDP levels improved the digestion of fibre i n the rumen, but this is unlikely to have been sufficient to have accounted for all of the response.

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Shi, Nan, Shuangya Wen, Qianwen Gao, Zhiqiang Gao et Huibing Yang. « Printed Sowing of High-Density Mechanical Transplanted Hybrid Rice Can Reduce the Amount of Fertilizer Needed ». Agronomy 12, n^o 9 (25 août 2022) : 2002. http://dx.doi.org/10.3390/agronomy12092002.

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In this study, we investigated how printed sowing machine transplanting impacts the yield of single-season rice by increasing the planting density and decreasing the amount of fertilizer needed. The study was aimed at exploring the relationships between the amount of fertilizer, transplanting density, and rice yield. During the rice growing season from 2019 to 2020 in the middle and lower reaches of the Yangtze River, six different field trials were conducted: low density and high fertilizer (LDHF), low density and low fertilizer (LDLF), middle density and high fertilizer (MDHF), middle density and low fertilizer (MDLF), high density and high fertilizer (HDHF), and high density and low fertilizer (HDLF). It turns out that compared to the LDHF, the thousand seed weight, the spikelets per panicle, the seed-setting rate, and the SPAD value at the filling stage decreased by 0.17% and 0.60%, 5.36% and 10.59%, 5.70% and 4.66%, and 17.52% and 4.93% in 2019 and 2020, respectively. However, compared to the LDHF, the panicles increased by 15.31% and 17.18%, respectively, the LAI at the filling stage increased by 1.92% and 0.48%, respectively, and the accumulation of dry matter above ground at the maturity stage also increased by 3.74% and 16.79% in 2019 and 2020, respectively. Therefore, compared to the yield of rice in the LDHF, the yield of rice in the HDLF increased by 5.06% and 6.64%. The yields of rice in the LDLF, MDHF, MDLF, and HDHF were lower than that in the LDHF and HDLF. The partial least squares path model (PLSPM) analysis showed that the fertilizer, density, and aboveground dry matter had positive effects on the yield, while the SPAD value and LAI had negative effects on the yield. This research shows that increasing the transplanting density can compensate for the yield loss caused by reducing the fertilizer amount. However, no combination of the transplanting density and fertilization amount can achieve the purpose of increasing the yield.

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Sanders, Akeisha N., Lori F. Wright et Martin S. Pavelka. « Genetic characterization of mycobacterial l,d-transpeptidases ». Microbiology 160, n^o 8 (1 août 2014) : 1795–806. http://dx.doi.org/10.1099/mic.0.078980-0.

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l,d-Transpeptidases (Ldts) catalyse the formation of 3–3 cross-links in peptidoglycans (PGs); however, the role of these enzymes in cell envelope physiology is not well understood. Mycobacterial PG contains a higher percentage of 3–3 cross-links (~30–80 %) than the PG in most other bacteria, suggesting that they are particularly important to mycobacterial cell wall biology. The genomes of Mycobacterium tuberculosis and Mycobacterium smegmatis encode multiple Ldt genes, but it is not clear if they are redundant. We compared the sequences of the Ldt proteins from 18 mycobacterial genomes and found that they can be grouped into six classes. We then constructed M. smegmatis strains lacking single or multiple Ldt genes to determine the physiological consequence of the loss of these enzymes. We report that of the single mutants, only one, ΔldtC (MSMEG_0929, class 5), displayed an increased susceptibility to imipenem – a carbapenem antibiotic that inhibits the Ldt enzymes. The invariant cysteine in the active site of LdtC was required for function, consistent with its role as an Ldt. A triple mutant missing ldtC and both of the class 2 genes displayed hypersusceptibility to antibiotics, lysozyme and d-methionine, and had an altered cellular morphology. These data demonstrated that the distinct classes of mycobacterial Ldts may reflect different, non-redundant functions and that the class 5 Ldt was peculiar in that its loss, alone and with the class 2 proteins, had the most profound effect on phenotype.

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Hernández, Sara B., Sónia Castanheira, M. Graciela Pucciarelli, Juan J. Cestero, Gadea Rico-Pérez, Alberto Paradela, Juan A. Ayala et al. « Peptidoglycan editing in non-proliferating intracellular Salmonella as source of interference with immune signaling ». PLOS Pathogens 18, n^o 1 (25 janvier 2022) : e1010241. http://dx.doi.org/10.1371/journal.ppat.1010241.

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Salmonella enterica causes intracellular infections that can be limited to the intestine or spread to deeper tissues. In most cases, intracellular bacteria show moderate growth. How these bacteria face host defenses that recognize peptidoglycan, is poorly understood. Here, we report a high-resolution structural analysis of the minute amounts of peptidoglycan purified from S. enterica serovar Typhimurium (S. Typhimurium) infecting fibroblasts, a cell type in which this pathogen undergoes moderate growth and persists for days intracellularly. The peptidoglycan of these non-proliferating bacteria contains atypical crosslinked muropeptides with stem peptides trimmed at the L-alanine-D-glutamic acid-(γ) or D-glutamic acid-(γ)-meso-diaminopimelic acid motifs, both sensed by intracellular immune receptors. This peptidoglycan has a reduced glycan chain average length and ~30% increase in the L,D-crosslink, a type of bridge shared by all the atypical crosslinked muropeptides identified. The L,D-transpeptidases LdtD (YcbB) and LdtE (YnhG) are responsible for the formation of these L,D-bridges in the peptidoglycan of intracellular bacteria. We also identified in a fraction of muropeptides an unprecedented modification in the peptidoglycan of intracellular S. Typhimurium consisting of the amino alcohol alaninol replacing the terminal (fourth) D-alanine. Alaninol was still detectable in the peptidoglycan of a double mutant lacking LdtD and LdtE, thereby ruling out the contribution of these enzymes to this chemical modification. Remarkably, all multiple mutants tested lacking candidate enzymes that either trim stem peptides or form the L,D-bridges retain the capacity to modify the terminal D-alanine to alaninol and all attenuate NF-κB nuclear translocation. These data inferred a potential role of alaninol-containing muropeptides in attenuating pro-inflammatory signaling, which was confirmed with a synthetic tetrapeptide bearing such amino alcohol. We suggest that the modification of D-alanine to alaninol in the peptidoglycan of non-proliferating intracellular S. Typhimurium is an editing process exploited by this pathogen to evade immune recognition inside host cells.

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Gu, Henan, Jian Luo, Guofang Li, Yueling Yao, Yan Huang et Dongjing Huang. « Spatial-Temporal Variations of Active Accumulated Temperature and Its Impact on Vegetation NDVI in the Source Region of China’s Yellow River ». Water 14, n^o 21 (29 octobre 2022) : 3458. http://dx.doi.org/10.3390/w14213458.

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Global climate change has greatly influenced the ecosystems in the Tibetan Plateau. Many studies focused on the direct effects of climate warming on the headwater regions by mean temperature, while less investigating its implication for the eco-environment. To address this, the study discussed the spatial-temporal variations of the bio-related climate indicators ≥0 °C annual accumulated temperature AAT0 and its lasting days LDT0, and corresponding ≥5 °C indicators AAT5 and LDT5 on the source region of the Yellow River (SRYR). The stationarity of indicators during 1979–2018 were tested by Pettitt test, and trends checked by linear regression analysis and Mann-Kendall test. Normalized difference vegetation index NDVI (2001–2016) was adopted to detect the correlation between vegetation activities and indicators. Results show that the AAT and LDT0 exhibited significant increasing trend over the SRYR, while the LDT5 significantly increased mainly under 4000 m. Most LDT extended due to the combined efforts of the early onset and late termination of the given temperature. 1997 was detected in the abrupt change analysis of AAT0 both on the basin scale and most area, and was adopted to divide the period into two stages. The regional mean AAT0 linearly grew at a rate of 96 °C decade−1 during the entire period, and 104 °C decade−1 during the second stage. Except for a drastic jump in the areal mean values, there was a distinct upward-shift of isoline in elevation between stages. NDVI showed strong correlativity with ≥0 °C indicators on the basin scale, according to the Pearson, Spearman and Kendall correlation coefficients, ranging from 0.5 to 0.7. Spatially, the overlap area between Pearson’s γ ≥ 0.5 and linearly rising AAT0 reached 50%, which was fully covered with significantly increasing AAT0 during the recent stage. Thus the rapid growth of ≥0 °C indicators would effectively accelerate NDVI over this major alpine grasslands, especially around the eastern low regions, where indicators are higher and grow faster.

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Thèses sur le sujet "LdtF"

MORÈ, NICCOLÒ. « Lipopolysaccharide transport and peptidoglycan remodeling : two related processes in Escherichia coli ». Doctoral thesis, Università degli Studi di Milano-Bicocca, 2018. http://hdl.handle.net/10281/198942.

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Il rivestimento dei batteri Gram negativi consiste in una membrana interna (IM) e una membrana esterna (OM) separate da uno spazio periplasmatico contenente un sottile strato di peptidoglicano (PG) ancorato alla OM tramite la lipoproteina di Braun (LPP). Mentre la IM è costituita da un doppio strato di fosfolipidi, la OM è una membrana lipidica asimmetrica, contenente fosfolipidi nel foglietto interno, e un lipide complesso il Lipopolisaccaride (LPS) nel foglietto esterno. Il LPS sintetizzato nel citoplasma, viene traslocato sul lato periplasmatico della IM e preso in carico dal complesso multiproteico Lpt (LPS transport), composto in Escherichia coli da sette proteine essenziali (Lpt ABCDEFG) che si occupano del suo trasporto fino al raggiungimento della sua sede finale, la OM. Analisi biochimiche hanno dimostrato che le sette proteine Lpt formano un complesso “transenvelope” che connette IM e OM e studi di tipo genetico suggeriscono che esse operino in concerto come un singolo macchinario. Infatti, la deplezione di un qualsiasi componente del complesso Lpt causa lo stesso fenotipo, ovvero l’accumulo del LPS nel versante periplasmatico della IM, la decorazione del LPS con acido colanico e la formazione di una IM anomala, con una densità intermedia tra la IM e la OM. Nel nostro laboratorio è stata condotta l’analisi differenziale del proteoma delle membrane totali di E. coli in deplezione di LptC, per studiare la risposta globale al blocco del trasporto del LPS. Tra le proteine il cui livello cambia nel confronto tra il ceppo non depleto e il depleto sono state trovate proteine coinvolte nella biogenesi e nel rimodellamento del PG. Lo scopo di questa tesi è stato lo studio della correlazione tra il blocco del LPS e il rimodellamento del PG. Inizialmente è stata analizzata la struttura del PG in deplezione di LptC. Questa analisi ha evidenziato che in questa condizione la struttura del PG varia sia per composizione che per tipo di legami crociati tra i filamenti glicanici adiacenti. Nei batteri Gram negativi il legame tra i filamenti glicanici è generalmente un legame diretto 3-4, che si forma tra il gruppo aminico del diaminoacido in posizione 3 di un tetrapeptide e il gruppo carbossilico della D-alanina in posizione 4 del tretrapeptide adiacente. Il legame 3-4 avviene ad opera delle D,D transpeptidasi PBP. Un altro tipo di legame crociato presente nel PG è quello tipo 3-3 che si forma tra il gruppo aminico del diaminoacido in posizione 3 ed il gruppo carbossilico del diaminoacido presente nel tetrapeptide del filamento glicanico adiacente ed è catalizzato da L,D-transpeptidasi . In E. coli, sono noti cinque enzimi con attività L,D-transpeptidasica, di cui tre (LdtA, LdtB, LdtC) ancorano la lipoproteina più abbondante della OM (lipoproteina di Braun) al PG e due (LdtD, LdtE) catalizzano il legame crociato 3-3. La delezione di tutti questi geni, singolarmente o in combinazione, non presenta nessun fenotipo, suggerendo che in condizioni normali questo legame è dispensabile. Per studiare la correlazione tra il legame crociato 3-3 e il blocco del trasporto del LPS abbiamo creato mutanti arabinosio dipendenti per alcuni dei componenti del sistema Lpt deleti contemporaneamente per i geni che esprimono le L,D-transpeptidasi LdtD e LdtE. In precedenza, nel nostro laboratorio è stato dimostrato che la deplezione di uno qualsiasi dei geni lpt causa la formazione di cellule filamentose e l’arresto della crescita ma non la lisi cellulare. Invece, nei mutanti ΔldtDΔldtE, in deplezione dei geni lpt, oltre alla formazione di cellule filamentose si osserva la formazione di un setto anomalo e la lisi cellulare. Questi dati suggeriscono che il rimodellamento del peptidoglicano a seguito della formazione di legami 3-3 potrebbe essere una forma di riposta al danno alla membrana esterna.
The cell envelope of Gram-negative bacteria is a complex multi-layered structure consisting of a cytoplasmic and an outer membrane (CM and OM), which delimit the periplasm containing a thin layer of peptidoglycan (PG) called the sacculus. The primary function of the OM is to establish a selective permeability barrier that enables the cell to maintain favourable intracellular conditions even in hash environments and the lipopolysaccharide (LPS) layer greatly contributes to this peculiar property. The integrity of the PG mesh is essential to protect the cell from bursting due to its turgor and maintain the shape of the cell. OM and PG are synthetized and assembled by multiprotein machineries that need to be finely coordinated as imbalanced growth of these layers may compromise structural integrity of the cell. In order to gain more insight in the mechanism by which the cells coordinate the growth of these two layers, we analysed the PG composition when the biogenesis of OM is compromised due to the block of LPS transport. In this work we shown that when OM is impaired, E. coli cells remodel PG architecture by increasing the non-canonical 3- 3 cross-linkage. We can assume that this is a salvage mechanism to prevent cell lysis when OM is damaged.

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Broussot, Loïc. « Implication du noyau latérodorsal du tegmentum dans les réponses au stress adaptatives et mésadaptées ». Electronic Thesis or Diss., Université Côte d'Azur, 2020. http://theses.univ-cotedazur.fr/2020COAZ6014.

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La réponse comportementale au stress est un mécanisme adaptatif qui permet à l’organisme de gérer les stimuli environnementaux nocifs. Les circuits qui la sous-tendent peuvent cependant être dérégulés par un stress intense ou chronique, conduisant à des troubles neuropsychiatriques. L’aire tegmentale ventrale (ATV) est une région du mésencéphale qui joue un rôle crucial dans le traitement de la récompense et l’aversion. On sait qu’elle influence la réponse au stress, mais notre connaissance sur ce rôle reste limitée car on ignore la plupart des structures régulatrices qui le sous-tendent. Le noyau latérodorsal du tegmental (LDTg) est une structure du tronc cérébral qui régule l’activité de l’ATV. Si son influence sur la récompense a été largement décrite, son rôle dans la réponse au stress reste encore inconnu.Cette thèse explore le rôle du LDTg dans deux types de réponse au stress : 1) Les comportements défensifs adaptés face à un stress aigu. 2) Les troubles comportementaux mésadaptés résultant d’un stress chronique. Pour comprendre le rôle du LDTg dans chacune, nous avons utilisé des outils pharmacogénétiques et optogénétiques pour moduler son activité durant des situations stressantes et en observer les effets comportementaux. En administrant de courts chocs électriques, nous avons évalué le rôle du LDTg dans un comportement défensif adaptatif d’immobilisation appelé freezing. Nous avons inhibé sélectivement les projections du LDTg de différentes natures et ciblant différentes structures afin de montrer que les projections GABAergiques du LDTg vers l’ATV régulent le freezing de manière bidirectionnelle. De la même façon, l’utilisation d’outils pharmacogénétiques dans un modèle de souris soumis à un stress social chronique nous a permis d’évaluer le rôle du LDTg dans des comportements de type dépressif. Les projections cholinergiques, mais pas glutamatergiques, du LDTg vers l’ATV sont ainsi nécessaires à l’induction des troubles de type dépressif par un stress chronique.En conclusion, ces résultats nous ont permis de mettre en évidence un rôle nouveau de l’axe LDTg-ATV dans la réponse au stress. Il est impliqué à la fois dans les réponses défensives adaptatives et les troubles comportementaux liés au stress. Ces informations pourraient aider dans le futur au développement de nouvelles thérapies innovantes basées sur la modulation des circuits cérébraux
The behavioural stress response is an adaptive mechanism that allows an organism to cope with threatening environmental stimuli. However, the circuitries that underlie it can be deregulated by intense or chronic stress and lead to maladaptive neuropsychiatric disorders. The ventral tegmental area (VTA) is a midbrain region that plays a crucial role in reward and aversion processing. It also influences the stress response, but our understanding of its role is still limited as the regulatory inputs that shape its activity during stress remain largely unknown. The laterodorsal tegmental nucleus (LDTg) is a brainstem structure regulating VTA activity. While its influence on reward processing has been widely described, its role in the stress response has yet to be determined.This thesis explores the role of the LDTg in two types of stress responses: 1) Adaptive defensive behaviours under acute stress. 2) Maladaptive behaviours following chronic stress. To assess the role of the LDTg in both contexts, we used chemogenetic and optogenetic tools to modulate its activity in mice during stressful situations and observed how it altered their behavioural responses.By using acute electrical shocks, we measured the influence of the LDTg over the freezing defensive response, an adaptive behaviour in mice confronted to an unseen threat. By selectively inhibiting LDTg projections of various natures and to different targets, we unravelled a new GABAergic LDTg-VTA pathway that bidirectionally modulates freezing in response to acute stress. Similarly, we used selective chemogenetic silencing in a mouse model of chronic social defeat to assess the role of the LDTg in stress-related depressive-like disorders. Cholinergic, but not glutamatergic, LDTg projections to the VTA were sufficient and necessary to induce depressive-like disorders after chronic stress. Together, these results unravel a new role of the LDTg-VTA axis in the regulation of the stress response. We show it is involved in both adaptive defensive behaviours and maladaptive stress-related disorders through converging yet different pathways. This may open a new therapeutic window for innovative treatments based on cerebral modulation

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Bourke, Alexandre. « Étude de la désorption thermique laser sur un dépôt de polytétrafluoroéthylène dans une source ionique LDTD ». Thesis, Université Laval, 2009. http://www.theses.ulaval.ca/2009/26290/26290.pdf.

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Capone, Georgina. « Staff and service users' evaluations of therapeutic principles at a High Secure Learning Disability Therapeutic Community (LDTC) ». Thesis, University of Lincoln, 2017. http://eprints.lincoln.ac.uk/29721/.

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Background: Growing evidence has been provided on the efficacy of Democratic Therapeutic Community (DTC) treatment in forensic LD populations (known as learning disability therapeutic communities, LDTC) in the form of reduced violence, personality pathology and interpersonal difficulties. Recently, the LDTC model has been introduced within a high secure setting at one of three high secure hospitals in the U.K., for males with a dual diagnosis of mild LD and PD, and produced equally successful results. While a number of outcome studies exist, on-going difficulties have remained in regard to applying a post-positivist approach to research design of Therapeutic Communities (TCs) as the approach fails to capture its matrix of interrelated treatment components. Consequently, there has been a call for investigation of processes within DTCs to identify important treatment mechanisms that support therapeutic change. While Haigh (2013) has updated the theoretical background on DTCs via formulating ‘quintessential principles’ within a given therapeutic environment the principles have not been empirically validated within a TC setting. Study aims: To explore service user and staff members’ evaluations of the quintessence principles as outlined by Haigh (2013) and identify whether any further important principles exist within the social climate of the LDTC that were not captured by current TC theory. Design: A single case study design was employed, with the ‘case’ being defined as the LDTC based at one of three high secure hospitals in the U.K. A qualitative approach was employed within the case study to enable initial analysis of TC members’ experience of therapeutic principles, any additional principles and to also permit identification of any shared experiences. The results of the qualitative analysis were used to develop a set of statements that can be used by future research to determine the importance of existing TC principles and additional elements identified in qualitative findings to TC members. Method: A qualitative approach was employed to enable analysis of TC members’ experience and evaluation of therapeutic principles in addition to identification of shared experiences. Data were collected via semi-structured interviews with 12 participants (6 staff members and 6 service users). The interview transcripts were initially analysed via deductive content analysis (Mayring, 2001) in order to identify whether Haigh’s (2013) quintessence principles were evident in the LDTC. Inductive thematic analysis (Braun & Clarke, 2006) was then performed on remaining data, which also involved completion of saliency analysis (Buetow, 2010) in the final stage to justify selection of themes and ensure identification of codes that did not recur but remained important to the research questions posed. Results: The deductive content analysis identified all five quintessence principles were experienced in the LDTC environment by staff and service users. Some limits to the principle of ‘agency’ were highlighted, with specific reference to difficulties implementing a flattened hierarchy in a forensic setting. Additional themes were identified via inductive thematic analysis and a saliency analysis indicated the following themes as both important and recurrent; security and risk, responsivity, trust, more physical freedom. Further themes that were identified as important but not recurrent within the saliency analysis included: staff fit with LDTC, moving on, being reflective. The theme of security and risk was specifically related to the context of the LDTC functioning in a high secure environment and ‘trust’ was understood to fall within Haigh’s (2013) conceptualization of the containment quintessence principle. While the remaining themes may not primarily contribute to the experience of secondary emotional development outlined by Haigh’s (2013) five quintessence principles they remain important considerations within therapeutic environments in light of their role in facilitating enactment of TC principles within secure environments, such as the LDTC. Conclusions: This is the first research paper that has attempted to test whether Haigh’s (2013) quintessence principles are evident within a given therapeutic community. The single case study provides empirical evidence for the quintessence principles in a novel TC setting along with further elements in the environment that help support implementation of quintessence principles. Fundamentally, the study suggests important recommendations for future research.

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Landes, Jasmin K. « Hemispheric differences in the temporal updating in short narrative situation models using a LDT ». Thesis, Landes, Jasmin K. (2011) Hemispheric differences in the temporal updating in short narrative situation models using a LDT. Honours thesis, Murdoch University, 2011. https://researchrepository.murdoch.edu.au/id/eprint/10608/.

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Recent research into situation model representations has demonstrated the neglect of the temporal dimension relative to the remaining dimensions that govern the formation of situation model representations. Furthermore, literature has recently demonstrated right hemisphere (RH) involvement in the processing, integrating and revising of semantic information. For the purpose of gaining a cohesive understanding of situation models and the mechanisms involved in their formation, the present study aimed to investigate whether the RH hosts at least the temporal dimension of situation model representations. Thirty-four right-handed psychology students from Murdoch University participated in a computerised go-nogo lexical decision task (LDT) in which participant reaction time and error rates were documented. Temporal shifts in situation model representations were controlled for by presenting participants with short narrative passages that included short or long temporal adverbials or none at all during baseline/ neutral condition. Words and non-words were projected to the left visual field (LVF)/RH, the central visual field (CVF) and the right visual field/ left hemisphere (RVF/LH). Contrary to the hypotheses, the results did not demonstrate any temporal shifts for targets presented to the CVF or LVF/RH, as the degree of facilitation of targets between the short and long temporal references did not vary significantly. These findings are contrary to Zwaan’s (1996) strong iconicity assumption as well as previous behavioural research to suggest temporal updating. Nonetheless, compelling theoretical support lead to the continued maintenance of the claim that temporal awareness is inherent to the LVF/RH.

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Melo, Juraj. « Aplikace pro tvorbu sad testů GUI ». Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2013. http://www.nusl.cz/ntk/nusl-236402.

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This thesis describes a system for automated GUI testing using assistive technologies for accessing and manipulating GUI elements. The only input from the user to automated test system is a description of UI events and activities. For this purpose, a specialized language is proposed. The test system then automatically generates possible sequences of UI events applying a given criterion. Generated test set is executed by Python interpreter exploiting the Linux Desktop Testing Project (LDTP). Test system described in this thesis then provides reports and coverage evaluation for particular test cases and the whole test set.

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Chapman, Valerie M. « A detailed archaeological analysis of LDF-030D (47Vi257) of the Lac du Flambeau Reservation, Vilas County, Wisconsin / ». Connect to online version, 2008. http://minds.wisconsin.edu/handle/1793/36631.

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Bergstrand, Sara. « Tissue Blood Flow Responses to External Pressure Using LDF and PPG : Testing a System Developed for Pressure Ulcer Research ». Licentiate thesis, Linköping : Department of Medical and Health Sciences, Linköping University, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-51886.

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Paley, M. T. « The development of an automated optical scanning system and studies of the Earth orbital dust environment by means of the LDEF Micro Abrasion Package ». Thesis, University of Kent, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.282485.

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Blachon, Gregory. « Détection et quantification du chloramphénicol dans le miel par thermodésorption laser à la pression atmosphérique couplée à la spectrométrie de masse tandem (LDTD-APCI-MS/MS) ». Thesis, Université Laval, 2011. http://www.theses.ulaval.ca/2011/28066/28066.pdf.

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Livres sur le sujet "LdtF"

United States. National Aeronautics and Space Administration., dir. LDEF materials/contamination. [Washington, DC : National Aeronautics and Space Administration, 1997.

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L, Colborn B., et United States. National Aeronautics and Space Administration., dir. LDEF satellite radiation study. Prospect, Tenn : SAIC, Science Applications International Corporation, 1994.

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Funk, Joan G. LDEF materials data bases. Hampton, Va : National Aeronautics and Space Administration, Langley Research Center, 1993.

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A, Stein Bland, Young Philip R et United States. National Aeronautics and Space Administration. Scientific and Technical Information Program, dir. LDEF materials workshop '91. [Washington, DC] : National Aeronautics and Space Administration, Office of Management, Scientific and Technical Information Program, 1992.

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Center, Langley Research, dir. Manual for LDEF tensile tests. Hampton, Va : National Aeronautics and Space Administration, Langley Research Center, 1985.

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V, Benton E., Heinrich Winfried et United States. National Aeronautics and Space Administration., dir. Ionizing radiation exposure of LDEF. [Washington, DC : National Aeronautics and Space Administration, 1990.

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Garland, Gouger H., et Langley Research Center, dir. LDEF, a bibliography with abstracts. Hampton, Va : National Aeronautics and Space Administration, Langley Research Center, 1992.

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British Institute of Learning Disabilities., dir. BILD LDAF programme : Mandatory assessment booklets. Kidderminster : BILD, 2002.

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O'Neal, Robert L. Photographic survey of the LDEF mission. Hampton, Va : National Aeronautics and Space Administration, Langley Research Center, 1996.

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O'Neal, Robert L. Photographic survey of the LDEF mission. Hampton, Va : National Aeronautics and Space Administration, Langley Research Center, 1996.

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Chapitres de livres sur le sujet "LdtF"

Zhong, Maiying, Ting Xue, Steven X. Ding et Donghua Zhou. « LDTV Systems and Fault Detection and Estimation for LDTV Systems ». Dans Fault Diagnosis for Linear Discrete Time-Varying Systems and Its Applications, 31–47. Singapore : Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-5438-2_3.

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Singer, S. F., J. E. Stanley et P. Kassel. « The LDEF Interplanetary Dust Experiment ». Dans Properties and Interactions of Interplanetary Dust, 117–20. Dordrecht : Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5464-9_24.

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Zhong, Maiying, Ting Xue, Steven X. Ding et Donghua Zhou. « Optimal Fault Detection for LDTV Systems ». Dans Fault Diagnosis for Linear Discrete Time-Varying Systems and Its Applications, 89–103. Singapore : Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-5438-2_6.

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Belov, Alexey A., Olga G. Andrianova et Alexander P. Kurdyukov. « Anisotropy-Based Analysis of LDTI Descriptor Systems ». Dans Control of Discrete-Time Descriptor Systems, 61–97. Cham : Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-78479-3_3.

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Mintmire, J. W. « LDF Electronic Structure of Fullerene Tubules ». Dans Density Functional Theory of Molecules, Clusters, and Solids, 153–75. Dordrecht : Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-011-0487-6_6.

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Zhong, Maiying, Ting Xue, Steven X. Ding et Donghua Zhou. « Parity Space-Based Fault Estimation for LDTV Systems ». Dans Fault Diagnosis for Linear Discrete Time-Varying Systems and Its Applications, 227–38. Singapore : Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-5438-2_15.

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Zhong, Maiying, Ting Xue, Steven X. Ding et Donghua Zhou. « $$H_{2}$$-Optimization-Based Fault Detection for LDTV Systems ». Dans Fault Diagnosis for Linear Discrete Time-Varying Systems and Its Applications, 63–88. Singapore : Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-5438-2_5.

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Zhong, Maiying, Ting Xue, Steven X. Ding et Donghua Zhou. « Stationary Wavelet Transform Aided Fault Detection for LDTV Systems ». Dans Fault Diagnosis for Linear Discrete Time-Varying Systems and Its Applications, 255–71. Singapore : Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-5438-2_17.

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Steel, Duncan. « Face-Dependent Impact Probabilities Upon LDEF for Heliocentric Particle Orbits ». Dans Origin and Evolution of Interplanetary Dust, 41–44. Dordrecht : Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3640-2_8.

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Zhong, Maiying, Ting Xue, Steven X. Ding et Donghua Zhou. « A Projection-Based Method of Fault Detection for LDTV Systems ». Dans Fault Diagnosis for Linear Discrete Time-Varying Systems and Its Applications, 105–19. Singapore : Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-5438-2_7.

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Actes de conférences sur le sujet "LdtF"

Buchanan, Frank P. « A Lightwave Digital Test Facility (LDTF) ». Dans 1985 Albuquerque Conferences on Optics, sous la direction de Susanne C. Stotlar. SPIE, 1985. http://dx.doi.org/10.1117/12.976145.

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Johnstone, Adrian, Elizabeth Scott et Mark van den Brand. « LDT ». Dans the Eleventh Workshop. New York, New York, USA : ACM Press, 2011. http://dx.doi.org/10.1145/1988783.1988792.

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De Giacomo, Giuseppe, Antonio Di Stasio, Francesco Fuggitti et Sasha Rubin. « Pure-Past Linear Temporal and Dynamic Logic on Finite Traces ». Dans Twenty-Ninth International Joint Conference on Artificial Intelligence and Seventeenth Pacific Rim International Conference on Artificial Intelligence {IJCAI-PRICAI-20}. California : International Joint Conferences on Artificial Intelligence Organization, 2020. http://dx.doi.org/10.24963/ijcai.2020/690.

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We review PLTLf and PLDLf, the pure-past versions of the well-known logics on finite traces LTLf and LDLf, respectively. PLTLf and PLDLf are logics about the past, and so scan the trace backwards from the end towards the beginning. Because of this, we can exploit a foundational result on reverse languages to get an exponential improvement, over LTLf /LDLf , for computing the corresponding DFA. This exponential improvement is reflected in several forms of sequential decision making involving temporal specifications, such as planning and decision problems in non-deterministic and non-Markovian domains. Interestingly, PLTLf (resp., PLDLf ) has the same expressive power as LTLf (resp., LDLf ), but transforming a PLTLf (resp., PLDLf ) formula into its equivalent LTLf (resp.,LDLf) is quite expensive. Hence, to take advantage of the exponential improvement, properties of interest must be directly expressed in PLTLf /PLDLf .

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KINARD, WILLIAM, et ROBERT O'NEAL. « Long Duration Exposure Facility (LDEF) results ». Dans 29th Aerospace Sciences Meeting. Reston, Virigina : American Institute of Aeronautics and Astronautics, 1991. http://dx.doi.org/10.2514/6.1991-96.

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Li, Ji, et Gary J. Cheng. « Effect of Multiple Pulses on the Deformation Behavior of Ultrathin Metal Foils in 3D Micro-Scale Laser Dynamic Forming ». Dans ASME 2010 International Manufacturing Science and Engineering Conference. ASMEDC, 2010. http://dx.doi.org/10.1115/msec2010-34299.

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Laser dynamic forming (LDF) is a novel high energy rate microfabrication technique, which makes use of the shock pressure induced by laser to generate dynamic high strain rate 3D forming of thin films. In LDF process, a high shock pressure accelerates the workpiece to a high velocity and deforms it into complex 3D shapes. The forming velocity of the workpiece imparted by a single laser pulse with high energy may exceed the critical forming velocity of the material, and thus causing it to fracture. This problem is more severe when 3D structure of large aspect ratio needs to be formed. To overcome this problem, multi-pulse laser dynamic forming is investigated in this study. The total laser energy is evenly distributed in different laser pulses to keep the forming velocity below the critical forming velocity of the material. The effects of the multiple-pulse LDF on the deformation behavior of ultra thin foils are investigated. The deformation depth and thickness variation distribution of the formed 3D features are characterized to reveal these effects. In addition, the effects of vacuum conditions on multiple-pulse LDF process are carried out. It is found that the bounce off of the foil can be effectively reduced by multiple-pulse LDF and the final shape could be controlled much more accurately. By extending single pulse LDF to multi-pulse LDF, the forming capability of LDF is further enhanced, and thus enlarges the applicable range of this technique.

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Raymond, Alex, et Srinivas Garimella. « Use of the Linear Driving Force Approximation in Adsorption Heat Pump and Chiller Modeling ». Dans ASME 2009 Heat Transfer Summer Conference collocated with the InterPACK09 and 3rd Energy Sustainability Conferences. ASMEDC, 2009. http://dx.doi.org/10.1115/ht2009-88426.

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Adsorption heat pumps and chillers can utilize solar or waste heat to provide space conditioning, process heating or cooling, or energy storage. In these devices, accurate modeling of intraparticle adsorbate mass transfer is an important part of predicting overall performance. The linear driving force (LDF) approximation is often used for modeling intraparticle mass transfer in place of the more detailed Fickian diffusion (FD) equation for its computational simplicity. This paper directly compares the adsorbate contents predicted by the conventional LDF approximation, an empirical LDF approximation proposed by El-Sharkawy et al. [1], and the FD equations for cylindrical adsorbent fibers such as activated carbon fiber (ACF). The conditions under which the LDFs agree with the FD equation are then evaluated. It is shown that for a given working pair, agreement between the LDF and FD equations is affected by the diffusivity, particle radius, half-cycle time, initial adsorbate content, and equilibrium adsorbate content. The maximum possible error in adsorbate content predicted by the LDF approximation compared with the FD solution is then calculated for the ACF (A-20)-ethanol working pair. Although the maximum error will be different for other cases, the technique used in this paper can be reproduced to determine the greatest possible LDF error for any working pair.

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Gao, Huang, et Gary J. Cheng. « Laser Induced High-Strain-Rate Superplastic 3D Micro-Forming of Metallic Thin Film ». Dans ASME 2009 International Manufacturing Science and Engineering Conference. ASMEDC, 2009. http://dx.doi.org/10.1115/msec2009-84087.

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Microforming of metals has always been a challenge because of the limited formability of metals at micro-scales. This paper investigates an innovative micro-forming technique: Laser Dynamic Forming (LDF), which induces 3-D superplastic forming in metal thin films. This forming process proceeds in a sequence of laser irradiation of ablative coating, ionization, shockwave generation and propagation in metal thin films, and conformation of metal thin films to the shape of micro/nanoscale molds. Because the deformation proceeds at ultrahigh strain rate, it is found that materials experience superplastic deformation at microscales. In this paper, experiments are carried out to understand the deformation characteristics of LDF. The shapes of the formed samples are characterized by scanning electron microscopy (SEM) and optical profilometer. The thickness variations are characterized by slicing the cross section using focused ion beam (FIB). The magnitude of deformation depth in LDF is determined primarily by three critical factors: thin film thickness, geometry of molds, and laser intensity. The relationships between laser intensity, film thickness, and mold size are explored in process maps to find out suitable processing conditions of LDF. Nanoindentation testings are conducted to show that the mechanical properties (hardness and yield strength) are increased significantly after LDF.

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Brafman, Ronen I., et Giuseppe De Giacomo. « Planning for LTLf /LDLf Goals in Non-Markovian Fully Observable Nondeterministic Domains ». Dans Twenty-Eighth International Joint Conference on Artificial Intelligence {IJCAI-19}. California : International Joint Conferences on Artificial Intelligence Organization, 2019. http://dx.doi.org/10.24963/ijcai.2019/222.

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In this paper, we investigate non-Markovian Nondeterministic Fully Observable Planning Domains (NMFONDs), variants of Nondeterministic Fully Observable Planning Domains (FONDs) where the next state is determined by the full history leading to the current state. In particular, we introduce TFONDs which are NMFONDs where conditions on the history are succinctly and declaratively specified using the linear-time temporal logic on finite traces LTLf and its extension LDLf. We provide algorithms for planning in TFONDs for general LTLf/LDLf goals, and establish tight complexity bounds w.r.t. the domain representation and the goal, separately. We also show that TFONDs are able to capture all NMFONDs in which the dependency on the history is "finite state". Finally, we show that TFONDs also capture Partially Observable Nondeterministic Planning Domains (PONDs), but without referring to unobservable variables.

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Anderson, Mark S., et Carl R. Maag. « Study of LDEF particulate contamination using atomic force microscopy ». Dans San Diego, '91, San Diego, CA, sous la direction de Gordon S. Kino. SPIE, 1992. http://dx.doi.org/10.1117/12.134888.

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Rantanen, Raymond, Tim Gordon, Miria M. Finckenor et Harold G. Pippin. « Comparison of contamination model predictions to LDEF surface measurements ». Dans SPIE's International Symposium on Optical Science, Engineering, and Instrumentation, sous la direction de Philip T. C. Chen, William E. McClintock et Gary J. Rottman. SPIE, 1998. http://dx.doi.org/10.1117/12.328498.

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Rapports d'organisations sur le sujet "LdtF"

Winn, W. G. Gamma-ray spectrometry of LDEF samples. Office of Scientific and Technical Information (OSTI), janvier 1991. http://dx.doi.org/10.2172/5637883.

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Winn, W. G. Gamma-ray spectrometry of LDEF samples. Office of Scientific and Technical Information (OSTI), décembre 1991. http://dx.doi.org/10.2172/10131992.

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Good, G. The LDAP Data Interchange Format (LDIF) - Technical Specification. RFC Editor, juin 2000. http://dx.doi.org/10.17487/rfc2849.

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Winn, W. G. Gamma ray spectrometry of LDEF samples : Results of 1992 analyses. Office of Scientific and Technical Information (OSTI), septembre 1993. http://dx.doi.org/10.2172/10188328.

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Brereton, S. J. Methodology for the relative risk assessment in the LDF safety analysis report. Office of Scientific and Technical Information (OSTI), septembre 1997. http://dx.doi.org/10.2172/591783.

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