Articles de revues sur le sujet « LC. Internet, including WWW »

It is clear that the presence of an Islamic factor in WWW has become an objective reality, including in the post-Soviet Muslim countries. It could be determined as a Cyber-Islamic Environment (CIE). In the post-Soviet space the CIE is represented mostly by official Islamic structures, while the presence of unofficial religious organizations and individual preachers is more limited. Nevertheless the influence of the non-official CIE is significant and is capable to be converted into one of the mechanisms of social protests and revolutionary activities in post-Soviet Muslim countries in case of emergence of critical situations. It is clear that state authorities will accelerate control over the CIE in the coming years using various instruments against religious radicalism and extremism in WWW.

AbstractFourier coefficients are a valuable tool in the study of a wide variety of pulsating stars. They can be used to derive various physical parameters, including mass, luminosity, metallicity and effective temperature and are frequently used to discriminate between different pulsation modes. With the increase in large-scale surveys and the availability of data on the Internet, the number of Fourier coefficients available for study has expanded greatly and it is difficult to find all current data for individual stars or a subset of stars. To assist others in obtaining and making use of Fourier coefficients, an archive of published values of Fourier coefficients has been set up. Users can search for data on individual stars or for a range of parameters. Several Java programs are used to display the data in a variety of ways. The archive is located at the Web site http://www.earth.uni.edu/fourier/.

We are creating a World-Wide Web (WWW) introductory chemistry site that will incorporate many key features of an electronic chemistry curriculum, including flexibility, interactivity, extensibility, diversity, and self-assessment. The site will be accessible from browsers such as Mosaic and Netscape. Advantages of serving introductory chemistry from a WWW site include expanding access to chemistry instruction, platform independence, easing transitions between university units, and creating a curriculum responsive to diverse needs. Contributions and suggestions from the chemistry community can be incorporated easily and rapidly into the electronic curriculum. Since the WWW site could be used by any campus in the world having Internet access, we expect that our electronic curriculum will have a far-ranging impact on the way introductory chemistry is taught.

As more and more teacher preparation programs realize the need to include courses that deal with computer-assisted language learning, a crucial decision as to what is taught needs to be made, taking into consideration the various post-graduation goals ranging from teacher or teacher-trainer to researcher. Thus, the question of whether to go beyond teaching the potential uses of the computer and the Internet and to delve into how to create relevant computer-based tasks using the WWW and to train teachers in the use of advanced web page development techniques, including Flash, web-enabled databases, and the interactive possibilities of the WWW needs to be addressed. This paper tackles these issues and illustrates a sample approach in dealing with the grooming of the new generation of CALL researchers and practitioners. What can be accomplished during the first year of an MATESL program that highlights the importance of technology is explored through a reflective questionnaire, a computer capabilities matrix, and an analysis of web-based course projects. Nine first-year M.A. students participated in the study, which revealed that despite widely differing initial computer and Internet expertise among these future teachers and researchers, their knowledge of creating and using web-based materials increased significantly, culminating in a project in which the skills acquired throughout the semester were successfully integrated.

One of the areas of development of the World Wide Web that requires complex scientific research is data and content management. This industry contains such areas as big data management, cloud-based data management, multimedia data management, and is also involved in Web-mining, the tasks of clustering, classification and analysis of data in the Web, Web content modeling, Semantic Web, etc. The task of effective Web content management has become very important for many industries that have emerged on the basis of the WWW infrastructure, including Internet commerce, distance learning and educational Web resources, managing large information portals, developing and maintaining corporate portals, creating Web-resources electronic management, supporting personal sites and blogs.

The Internet offers many new and unique opportunities to disseminate information. The development of the World Wide Web (WWW) and information browsers like Netscap, Mosaic, and simple-to-use server software like MacHTTP provides means to allow low-cost access to information, including pictures and graphics previously unavailable to most people. The Pennsylvania State Univ. variety trial garden annually tests >1000 plants. Information is gathered on garden and pack performance, and photos of superior plants and varieties are taken. To provide wider access to this information, we have begun development of a Cyberspace trial garden on the internet. This server contains a wide variety of garden trial information developed from trials conducted in State College and Dauphin, Pa.. This server and a similar effort at Univ. of Minnesota are being constructed cooperatively. Hot links are provided between the server in Pennsylvania and the one in Minnesota, providing users with seamless access to information from both servers.

AbstractThis article aims to give a broad outline of what the JISC Legal Information Service is and why it was set up. In particular it deals with the challenges of the Internet and the new legislation that attempts to regulate it or stimulate its use. The Internet is fast becoming embedded in the FE/HE sector, in teaching and learning, research and administration–ignore it at your peril some might say. Yet there are risks to this, in some cases, enthusiastic rush to embrace all things that utilise the WWW. The relative ease of access, extraction and dissemination on ‘the net’ has tempted some to overlook the legalities. This article summarises briefly the main areas of law under consideration and the potential costs of infringement. It explains that FE/HE is subject to this legislation sometimes in specific ways. It concludes by stressing the need for feedback from the sector including BIALL members in order that the service can improve.

E-learning is a model of learning carried out through the utilization of information and communication technology services such as internet, video/audio conferencing, or CD-ROM. It is a technology-based learning design of computer networks known as the world wide web (www). The design was developed through designing learning materials under a multimedia package and was integrated in a full online interactivity. Any form of learning components such as teaching or evaluation materials were distributed to learners through a computer network (online), so that learners who attended the program must be an IT literate person. In its implementation, e-learning could be applied to various fields of science including library science. Librarians who do such work require the ability and expertise to cataloging, indexing and classification. Web-based learning material helped students understand those materials easily. E-learning merupakan model pembelajaran yang dilakukan melalui pemanfaatan informasi dan layanan teknologi komunikasi seperti internet, konferensi video/audio, atau CD-ROM. E-learning merupakan suatu desain pembelajaran berbasis teknologi jaringan komputer yang dikenal sebagai world wide web (www). Desain ini dikembangkan melalui rancangan materi pembelajaran berdasarkan suatu paket multimedia terintegrasi dalam interaktivitas online. Segala bentuk komponen pembelajaran seperti bahan pengajaran atau evaluasi dibagikan kepada peserta didik melalui jaringan komputer (online), sehingga peserta didik yang mengikuti program ini haruslah orang yang melek IT. Dalam pelaksanaannya, e-learning dapat diterapkan pada berbagai bidang ilmu pengetahuan termasuk ilmu perpustakaan. Pustakawan yang melakukan pekerjaan seperti ini membutuhkan kemampuan dan keahlian untuk katalogisasi, pengindeksan dan klasifikasi. Materi pembelajaran berbasis web membantu siswa belajar dengan mudah.

Algorithmic versions of stand density management diagrams (SDMDs) were developed for natural and managed black spruce (Picea mariana (Mill.) B.S.P.) stands. Specifically, the IBM-compatible PC-based algorithms (1) graphically illustrate site-specific size-density trajectories for eight user-specified initial density regimes, (2) given (1), calculate and subsequent tabulate periodic yield estimates (mean dominant height, density, mean volume, total volume, total merchantable volume, quadratic mean diameter, and basal area), and (3) given (2), graphically illustrate empirically-derived yield production curves for total merchantable volume ha−1 and stems m−3 with user-specified operability criteria superimposed. Instructions on acquiring the executable algorithmic versions including the required graphical subroutines via the Internet are described. Currently, the algorithms are restricted in applicability to central insular Newfoundland. Key words: stand density management diagrams, black spruce, algorithms, microcomputer, World-Wide Web (WWW), hypertext browser, file transfer protocol (FTP).

As the World Wide Web (WWW) expands, information is rapidly becoming more accessible. Using satellite data previously required high-end computers running complex imaging software, sophisticated downloading equipment, and high monetary support. Satellite data is now available on the internet for little or no cost and can be handled on standard desktop computers using common software programs. The purpose of our project was to determine the availability and cost of different types of data and how this data may benefit horticultural instruction. Satellite data currently is archived at NASA, NOAA, the Department of Defense, the US Geological Survey, and various meteorological departments throughout the world. Satellite data such as large-scale thermal imagery can be used to determine microclimate effects within urban areas, including the cooling effects of urban plants. Natural Density Vegetation Index (NDVI) imagery can indicate changes in vegetational cover or give general indications of plant health in large areas. NASA photographic imagery can show the effects of erosion on a large scale. Higher resolution imagery can give indications of plant stresses in large plantings such as orchards or vegetable plots.

E-Commerce has been going on since Netscape.com introduced the idea in 1995 when www was invented. Businesses / consumers that have been immersed in e-commerce transaction have reaped the benefits associated with such technological break-through, as consumers sit at comfort of their homes to transact business. However, the impediment that has hindered other businesses / consumers to transform to this technological business approach has been the trust associated with carrying out business; consumer trust across global cultures has been contentious. Authors, including Hofstede, Gefen et al. and Greenberg et al. have done research on culture differences across the globe and how these differences could affect behaviours towards accepting e-commerce for transacting business. There is therefore, the need for a global digital guideline / policy to protect all consumers and businesses that trade on the internet. Such a policy would hopefully allay the fears amongst nations’ cultures having difficulty in imbibing this wholesome technological advancement for enhanced business transaction. Conducting business transaction through brick-and-mortar approach is archaic and cumbersome and should be faded out completely.

Context-aware advertising is one of the most critical components in the Internet ecosystem today because most WWW publisher revenue highly depends on the relevance of the displayed advertisement to the context of the user interaction. Existing research work focuses on analyzing either the content of the web page or the keywords of the user search. However, there are limitations of these works when being extended into mobile computing domain, where mobile devices can provide versatile contexts, such as locations, weather, device capability, and user activities. These contexts should be well categorized and utilized for online advertising to gain better user experience and reaction. This paper examines the aforementioned limitations of the existing works in context-aware advertising when being applied for mobile platforms. A mobile advertising system is proposed, using location tracking and context awareness to provide targeted and meaningful advertisement to the customers on mobile devices. The three main modules of this comprehensive mobile advertising system are discussed, including advisement selection, advertisement presentation, and user context databases. A software prototype that is developed to conduct the case studies and validate this approach is presented.

The Internet and World Wide Web (WWW) are evolving as an important communication technology. This paper aims to develop methodology for enabling better concurrent stamping part and die development, in the evolving Web technology. The characteristics of concurrent stamping part and die design are presented, and the necessity and feasibility of building Web-based concurrent stamping part and die development system is then discussed. Next, the overall architecture of Web-based concurrent stamping part and die design and development is established. The design services, which are the components of the Web server and play key roles in implementing the Web-based concurrent stamping part and die development, are then investigated in depth. Among the Web-based design services, the part shape analysis and feature-based cost evaluation are further illustrated in detail. While the system development approach including knowledge-based stamping part design and evaluation over Web is discussed, the cooperation and coordination between product designer and die designer are also presented with the illustration of a case study. The findings of this research allow the stamping part designer and the die designer to share product data, communicate with each other, and maintain operations consistency in the distributed cooperative sites on different product design and development platforms.

This work is part of a project to develop a test stand using a network simulator of ship traffic control and physical models of ship propulsion system components. The lab station is designed to allow control of the engine by three methods. Web-based control, slave-level control as well as master-level control. The components of the lab bench and the various blocks used in the TIA Portal program are described. The article deals with the application of Internet 4.0 to AC motor control. In order to be able to synthesize a laboratory workstation to enable the control capabilities of Internet 4.0, an idea diagram is described. The Profibus DP protocol was used to connect the I/O module to the PLC, which made it possible to control the operation of the motor directly from the module through the PLC connected to the inverter by the Modbus RTU protocol. In order to implement an experimental Internet 4.0 workstation, it was also necessary to add remote control using the HTTP protocol and design a website. All the above-mentioned protocols were briefly discussed and implemented in the TIA Portal environment. Also described is the idea and operation of a program written in the LAD language, which is designed to control an AC motor from three different devices: PLC, I/O module and PC via a web page. In the construction of the program, the MB_COMM_LOAD and MB_MASTER blocks dealing with communication and control with the inverter were used, as well as the WWW block used to communicate the controller with the website. The operation of the software was verified from the level of two devices, the I/O module and the website, including its optimization for the correct operation of the entire system as shown in the graph of the change in the value of the AC motor speed control word. In the final part of the work, an analysis of the suitability and validity of using such a system in industry was carried out.

Resnu Mauliyana Mukti Wilutomo, Teguh Yuwono, 3 phase induction motor must work well and safely. Many types of interference have the potential to interfere with motor performance or even damage the motor itself, including due to power instability which includes imbalance between phase voltages and more phase currents. Monitoring an industrial activity especially monitoring the protection system is very important, it is intended that the disturbance that occurs in the 3 phase induction motor can be analyzed for the value and type of interference. Rapid technological development requires a monitoring system that is practical, fast and accurate. Sensors installed on this device are current sensors, voltage sensors, and speed sensors. The read, current, voltage and speed will be used as input signals to Arduino using the ADC (Analog to Digital Converter) process, Arduino programming uses C ++ language. ESP8266 wifi module) will upload the data from the ADC (Analog to Digital Converter) process to the database which is then displayed on the web using the available internet network. The web programming language used is PHP. This tool makes it easy to monitor a 3 phase induction motor because it can be done anytime and anywhere. There was a difference of ± 0.24A between the Tang Ampere measuring instrument and reading on the web. A ± 4V difference occurs between the Multimeter measuring instrument with readings on the Web. The maximum speed of the 3-phase induction motor with no load is 22Rpm between the tachometer gauge and the web display. Keywords: voltage sensor, current sensor, speed sensor, C ++ language programming, PHP.References Mahardika, Ferdina . 2016. Rancang Bangun Alat Memonitor Ketidakstabilan Daya Berbasis Arduino Mega 2560 Pada Motor Induksi 3 Fasa. Semarang : Laporan Tugas Akhir Teknik Elektro Universitas Diponegoro.2. Girsang, Irma Sika. 2015. Perancangan Monitoring Jarak Jauh Ketinggian Air Pada Bendungan Menggunakan Sistem Android Via Jaringan Wi-Fi. Sumatera utara : Jurnal Ilmiah Ekstensi Fisika Instrumentasi FMIPA Universitas Sumatera Utara.Santoso, Imam , Rizal Isnanto, R, Chaerodin, Achmad. 2008. Sistem Monitoring Suhu Berbasis Web Dengan Akuisisi Data Melalui Port Paralel PC. Semarang : Laporan Tugas Akhir Teknik Elektro Universitas Diponegoro.Zuhal. 2000. Dasar Teknik Tenaka Listrik dan Elektronika Daya. Jakarta : Gramedia Pustaka UtamaArduino. Arduino due. 12 Mei 2017 pukul 21.40.( https://www. arduino.cc/en /Main/ arduino BoardDue ).Kho, Dickso . Pengertian dan prinsip kerja optocoupler. 12 Mei 2017 pukul 20.20. (http: //teknikelektronika .com / pengertian- optocoupler- fungsi- prinsip- kerja- optocoupler).Quang, Sáng Bùi. Rotary Encoder Training Material. 12 mei 2017 pukul 22.15. (https: //www .slideshare.net /sangbuiquang3 /rotary-encoder -training- material).Sugito, Tega.2016. Rancang Bangun Pengasutan Star Delta Pada Motor Induksi Tiga Fasa Berbasis Sensor Kecepatan Menggunakan Mikrokontroler Atmega 16 .Semarang : Laporan Tugas Akhir Teknik Elektro Universitas DiponegoroWidiyaman, Tresna . Pengertian Modul Wifi ESPN8266. 11 Mei 2017 pukul 16.50. ( http: // www. Warriornux .com / pengertian – modul - wifi - esp8266/).Labradoc. ESP8266 WiFi Module Quick Start Guide. 10 Mei pulul 17.40. (http://www.labradoc.com/i/follower/p/notes-esp8266).Prasetio, Andhi. 2012. Buku Pintar Pemrograman Web. Jakarta: Media K.Hidayat, Rahmat. 2010. Cara Praktis Membangun Website Gratis. Jakarta: PT Elex Media Komputindo.Fathansyah. 2012. Basis Data. Bandung: Informatika Bandung.

Software-defined radio (SDR) is a good solution for complying with the existing and incoming protocols for emerging wireless sensor networks (WSN) and internet of things (IoT) applications. The frequency synthesizer in a SDR tranceiver usually consists of a phase locked loop (PLL) and a post synthesizer. The PLL is the narrow band signal source and the post synthesizer generates wideband outputs by mixing and dividing. Compared with a frequency synthesizer utilizing the wideband PLL, this synthesizer features relatively constant loop parameters and mitigates the requirement for the oscillator. In this paper, a quadrature single side-band (QSSB) mixer with the proposed passive negative resistance (PNR) for frequency mixing in a post synthesizer is presented. The PNR is achieved by biasing the Metal-Oxide-Semiconductor Field-Effect Transistors (MOSFET) of the cross-coupled pair at the deep-triode region periodically and incorporates an inductor and a cap-array as the mixer load. Compared with the traditional single side-band mixers utilizing Inductor-Capacitor (LC) resonant loads or quality factor enhanced (Q-enhanced) LC resonant loads, which suffer from a selectivity versus working range trade-off, the mixer employing the proposed loading structure provides not only a wide operating range, but also a superior image side-band rejection ratio (ISRR). Moreover, the oscillating risk in conventional mixers adopting Q-enhanced LC resonant loads is eliminated. A wideband frequency synthesizer employing the proposed mixer was implemented in a TSMC 0.18 µm CMOS process and the mixer performed ISRR of 40–57 dB and 30–57 dB across 2.5–3 GHz and 2.3–3.2 GHz, respectively. The power consumption of the QSSB mixer, including buffer, is 18 mA from a 1.8 V supply and the active area is 0.445 mm2. The measurement results provide validation that the proposed QSSB mixer is suitable for wideband software-defined frequency synthesizers and other frequency generating systems.

Abstract The purpose of this study was to develop an LC-tandem MS method for the simultaneous detection of common synthetic drugs as adulterants in natural and herbal slimming products. Sixteen drugs belonging to a wide range of pharmaceutical classes were studied. Included in the list of drugs were anorexics, anxiolytics, antidepressants, diuretics, laxatives, and stimulants. The method used a C18 column (4.6 × 50 mm and 1.8 μm particle size). Separation of the drugs was achieved by gradient elution using 4 mM ammonium formate in water + 0.1% formic acid as the aqueous component and 4 mM ammonium formate in methanol + 0.1% formic as the organic component of the mobile phase. As not all of the analytes ionized in the positive mode, the mass spectrometer was operated in the electrospray ionization mode with polarity switching. The samples were extracted with methanol and the use of 50% acetonitrile in water and 50% methanol in water were investigated as diluents for injection into the LC-MS system. Utilizing both diluents, the validation parameters including accuracy, precision, LOD, and LOQ were assessed. The validation results and utilization of the method to analyze a variety of weight-loss supplements indicate that the two diluents give similar results and can be used interchangeably. This knowledge provides the user with the option of selecting either diluent for sample preparation depending on the sample matrix without having to revalidate the method. The method was applied to the analysis of weight-loss supplements available in local pharmacies, herbal pharmacies, and over the Internet.

ROMAN LAW ON THE INTERNETSummary In the past ten years the Internet has become a very popular information exchange tool serving people around the world. A summary review of information included on the world wide web indicates that the Internet constitutes a rich and diversified source of information about certain issues, which enables not only the popularisation of such knowledge, but also creates an open forum for the discussions of many different issues. There are also many sites on Roman on the Internet, which were created not only by the universities and other scientific centres, but also by private individuals interested in Roman law as a hobby. O f course, such web sites are either currently not very large and devoted to specific problems of Roman law, or very general and thus not of much use for romanists interested in specific issues. Also the catalogue of sources of Roman law that is available on the net is still incomplete, which probably results from the problems connected with the transformation of original source texts into electronic form. In this article I would like to present the results of my „web surfing”, in order to encourage Roman law researchers to use the Internet as a serious source of information, and to show that the Internet may provide enormous possibilities in the future. The various sites devoted to Roman law existing on the Internet may be divided into some general categories depending on the type and purpose of each of these sites.The first type or category consists of Internet sites created by universities, law school and other educational centres. The information included therein is mainly of an administrative nature and refers to the programme of studies, exam schedules, academic teachers and tutors and similar matters not connected with Roman law itself. Some of them also give information about special projects conducted in this particular school and information about local libraries with lists of available books. Apart from the private sites created by the members of the academic community on the web one can find also the sites created by individual people, who publish on the Internet the results of their research, their opinions on different legal problems connected with Roman law, summaries of books devoted to Roman law an so forth.The next category of Internet sites that may be used by a person studying Roman law are sites that include texts of legal sources. This kind of site, although not including much substantial information on Roman law, may be helpful for the researcher of antiquity and Roman law as it enables easy access to the text of selected source.Last of all I would like to pay some attention to Internet sites devoted to ancient Rome in general, not necessarily to Roman law. The sites of this kind are more popular science than strictly scientific materials, and probably they are not of much use for the historians of antiquity. On the other hand, they include some interesting pieces of information neither being taught in standard course of studies nor included in history manuals, bringing the realities of ancient world closer to us, such as information on Roman cuisine, Roman coins, or Roman clothes. These sites also include a large variety of pictures and photos, which makes them more attractive for visitors.As we can see the Internet has become quite a rich source of information about antiquity and Roman law. Taking into consideration all the advantages that this global network offers in the field of transferring and broadcasting information, certainly it is worthy of greater attention on the part of romanists. Since the information included therein is relatively general, the primary use of the Internet by romanists should be in my opinion as an educational tool. Encouraging students to use the Internet while learning Roman law may inspire them to more detailed studies on selected subjects, not limited to information included in popular manuals and, as the next step, in creating their own www sites devoted to particular problems in the field of Roman law. Simultaneously no less of importance is co-operation among romanists from all countries in order to make the Internet useful also for the researcher of Roman law. That could be achieved through placing texts of scholar books and articles on the web, creating the universities’ homepages devoted to Roman law, initialising collaborative Internet projects and presenting the individuals achievements in the field of Roman law on the net. As a result in a few years it could be expected that the Internet would become the compendium of information on Roman law, widely available and easy to use, as well as the forum of collaboration among academia in the field of Roman law. In the modern world, where history knowledge is often treated as an useless ballast, especially the researchers of antiquity should make use of technical innovations in the field of dissemination of information because it enables their knowledge to survive. Providing the virtual reality has become the constant element of everyday life, the reservation of space on the Internet for Roman law is the way to make Roman law in some sense „immortal”.

Electronic or e-trade trading (English: Electronic Commerce, also e-commerce) is a spread, purchase, sales, marketing goods and services through an electronic system such as internet or television, www, or other computer networks. Ecommerce can involve electronic fund transfers, electronic data exchange, auto inventory management systems, and automatic data collection systems. The Information Technology industry sees this e-commerce activity as the application and application of e-business (e-business) related to commercial transactions, such as: electronic fund transfer, SCM (E-Marketing), e-marketing), online marketing, online transaction processing (online transaction processing), electronic data interchange / edi), etc.E-commerce is part of e-business, where e-business coverage is wider, not just a commerce but includes also co-entrance business partners, customer service, job vacancies etc. Seeing very fast developments of the e-commerce growth in the world including in Indonesia, it is necessary an effective strategy for the tax authority in the reacting. One of the things that need to be concern is this very rapid growth should be maintained so that there is no distortion as a result of taxation policy. During this time, the tax aspect in e-commerce has been the highlight of tax authorities in the world, especially whether there should be a new tax impression on this transaction and also how to align the existing tax rules with e-commerce development.Transactions through digital media or e-commerce in Indonesia need rebuilding from the side of the legislation (Cyberlaw) so that in the future do not miss the dispute in it runs. This happens because the difficulty of tracking the transactions used through the e-commerece is either B-to-B (business to business) or B-to-C (business to consumers). For example the relationship between the supplier with the factory, how the apparatus is to oversee the existence of transactions or not between the two through the e-commerce the solution of solving the problem is the government must take steps in solving the unstable internet infrastructure issues and frequent reliable transportation, the licensing procedure of customs to dedicate to the duty procedure from dawn to the negative trade for the other value to increase the efficacy to make the rules for the epimal services that are still under the regulations of the same bank as well as the public of the transactions of online transactions, plan and conduct the introduction of certification systems, a number of large players should be able to open the way of foreign investment, including doing some major plans for foreign investors, of course with clear regulations and the government must provide ease of rules for small and medium business to gain access to investors, and giving taxes to the company in the new company.

Background:Osteoarthritis is a very common chronic disease. The information needs of patients vary depending on the health issue. Social media sites represent a novel source of health information and advice for patients with chronic diseases, such as osteoarthritis. Almost half of them use the internet to look for health related information [1]. No study has assessed the impact of social media on osteoarthritis and its treatment.Objectives:The purpose of this study was to evaluate frequently discussed osteoarthritis treatments on the social media Twitter.Methods:We retrospectively analyzed tweets, published between 1st and 31st January 2020, containing the keywords “osteoarthritis”. Only English language tweets were included. Tweets referred to veterinary medicine were excluded.Results:3587 tweets were analyzed. We identified 1737 tweets related to osteoarthritis treatment between 1st and 31st January 2020 (49.8%) (Figure 1). Dietary interventions were the most discussed treatment (18.3%, n=318/1737), including fruits, vegetables and plants (n=101), dietary supplements and vitamins (n=80), and spices (n=19). Physical medicine and rehabilitation (17.6%, n=305/1737) including sport (n=151), physiotherapy (n=70) and rehabilitation (n=57), were commonly discussed. Local therapies for osteoarthritis were also discussed by Twitter users (15.8%, n=274/1737). These included topical treatments such as anti-inflammatory gels and creams (n=23), and more invasive local treatments including intra-articular joint injections with corticosteroid (n=56), hyaluronans (n=29), stem cells (n=97), and Platelet-Rich Plasma (n=52). The frequently used systemic drugs were analgesia and non-steroidal anti-inflammatory drugs (n=113). Surgery and interventional radiology (genicular artery embolization) were also discussed (11.5%, n=199/1737). 5.6% tweets (n=97/1737) were related to alternative therapies. Predominant themes were related to marijuana (n=23), acupuncture treatment (n=17), homeopathy (n=10). Last but not least, 356 tweets (20.5%) referred to other websites including health programs.Conclusion:Our results demonstrate that osteoarthritis treatment is frequently discussed in published tweets. Thereby, social media could have an impact on behaviors and adherence on medication, and it seems interesting that learned societies, involved in osteoarthritis treatment, communicate more using social media.Figure 1.Osteoarthritis treatment discussed in published tweetsReferences:[1]Wagner TH, Baker LC, Bundorf MK, Singer S. Use of the Internet for health information by the chronically ill. Prev Chronic Dis 2004;1(4):A13.Disclosure of Interests:None declared

The article narratives about the necessity of religious education of a person with the help of the socializing environment of cyberspace, about religious education in the context of cybersocialization as a multidimensional process of development of spirituality that regulates human behavior in cyberspace in accordance with the moral-ethical bases of life that it has internalized, and the religious principles of a certain denomination. The role of religious resources of the World Wide Web in religious activities is substantiated. It is necessary to accept the cyberspace of Internet environment as a relatively independent virtual reality that dynamically develops in modern reality, in which it organizes its own cyber-life and cyber-socialization. Modern electronic technologies greatly transform the process of religious socialization, resulting in traditional agents leaving into the background, because Internet has now become a full-fledged institution of religious socialization of man. Now many tasks of religious development of man are carried out not only in traditional methods, but also in the context of the virtual space by attracting people to innovative technologies. Cyberspace contributes to the transformation of forms of expression of human religiosity. Today many tasks of religious development of a person are carried out not only by traditional methods, but also in the context of virtual space by bringing people into innovative technologies. The development of technology also affects world and new religions: in the XXI century WWW became a new means of spreading of the religious culture for them among its users. One of the trends of modern mass culture is the orientation of society into digital entertainment and communication in various social networks - this fact allows contemporary researchers to view the Global Web as a new social institution. It can be argued that the development of an innovative branch of psycho-pedagogical thought - cyber-pedagogy, which scientifically substantiates the purposeful and systematic activity of cyber-education of a modern person in the process of its cyber-socialization by means of computer and information-communication technologies that allows modern homo sapiens, who became homo cyberus, to learn to use socializing and educational opportunities of computer and Internet resources. The questions of necessity, multitasking and opportunities of religious education of a person in the context of cybersocialization are revealed. Religious education in the context of cybersocialization has a cyber-onthological character and is closely linked with the assimilated and positioned ideas by the personality in cyberspace about the value of human life, honor and dignity, spiritual wisdom, and the search for meaning in life. As rather serious, researchers evaluate the risks of network bullying - regular, prolonged and group harassment of the victim - for example, when against a pupil create a site or group in a social network, arrange offensive voting, steal information from his personal page and lay it in an obscene manner, ridicule publicly. The biggest problem in the fight against cybersquatting is the lack of legal skills for cyberbullying and the lack of clarity of legal consequences for an aggressor. It is advisable to develop an international mechanism for combating cybersquatting, including Interpol intervention, for creating a rehabilitation system for victims of cybersquatting based on advisory centers in the different regions of the country, and for developing legislative mechanisms that would clearly categorize and quickly remove aggressive content.

Plants undergo metabolic perturbations under various abiotic stress conditions; due to their sessile nature, the metabolic network of plants requires continuous reconfigurations in response to environmental stimuli to maintain homeostasis and combat stress. The comprehensive analysis of these metabolic features will thus give an overview of plant metabolic responses and strategies applied to mitigate the deleterious effects of stress conditions at a biochemical level. In recent years, the adoption of metabolomics studies has gained significant attention due to the growing technological advances in analytical biochemistry (plant metabolomics). The complexity of the plant biochemical landscape requires sophisticated, advanced analytical methods. As such, technological advancements in the field of metabolomics have been realized, aided much by the development and refinement of separatory techniques, including liquid and gas chromatography (LC and GC), often hyphenated to state-of-the-art detection instruments such as mass spectrometry (MS) or nuclear resonance magnetic (NMR) spectroscopy. Significant advances and developments in these techniques are briefly highlighted in this review. The enormous progress made thus far also comes with the dawn of the Internet of Things (IoT) and technology housed in machine learning (ML)-based computational tools for data acquisition, mining, and analysis in the 4IR era allowing for broader metabolic coverage and biological interpretation of the cellular status of plants under varying environmental conditions. Thus, scientists can paint a holistic and comprehensive roadmap and predictive models for metabolite-guided crop improvement. The current review outlines the application of metabolomics and related technological advances in elucidating plant responses to abiotic stress, mainly focusing on heavy metal toxicity and subsequent osmotic stress tolerance.

E-learning is aptly a practical response to continuous learning given the surge in the use of information technology, and economic disruptions impinging on the schools. The need to shift to e-learning has been exacerbated by the COVID-19 pandemic. In this regard, we sought to develop an organizational assessment instrument to internally ascertain the level of readiness of the school for sustainable e- learning in the new normal. This assessment instrument was primarily developed for the use of the Mendiola Consortium member schools in their pursuit to conduct elearning. We intended that as an internal self-assessment it can diminish the threat of failure and provide some assurance of the successful implementation of e-learning. We noted that many survey instruments had been made to assess organizational readiness as a construct for e-learning. However, it revealed that these instruments have varying limitations in validity and reliability to establish the domains of organizational readiness for e-learning. We anchored our study on the organizational readiness model developed by Schreurs and Al-Huneidi (2012) and Mercado (2002). From our review of related literature, we were able to generate seven basic dimensions of our model, namely: teacher, student, curriculum, technology, administrative support, financial support, and learning environment. We used a mixed method of qualitative and quantitative approach to come up with a validated instrument. We conducted a three-phase approach in developing the instrument. The final instrument yielded 45 items to be rated on a five-point Likert scale. For its content validity, the Item-Content Validity Index ranged from 0.91 to 0.96, while the Scale-Content Validity Index was 0.94. It has a Cronbach alpha of .975 for its reliability. ReferencesAlok , Kumar (2011). 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Questions and answers about testing statistics: Principal components analysis and exploratoryfactor analysis-Definitions, differences, and choices. Shiken: JALT Testing & Evaluation SIG Newsletter. 13(1), 26-30. Also retrieved from www at http://jalt.org/test/bro_29.htmBrown, J.D. (2009b). Statistics Corner. Questions and answers about testing statistics: Choosing the Right Type of Rotation in PCA and EFA. Shiken: JALT Testing & Evaluation SIG Newsletter. 13(2), 19-23. Also retrieved from www at http://jalt.org/test/bro_30.htmChapnick, S. (2000). Are you ready for e-learning? Learning Circuits: ASTD’s Online Magazine All About E-Learning. Retrieved May 21, 2011, from, http://www.astd.org/LC/2000/1100_chapnick.htmCoakes ,S.J (2013). SPSS: Analysis without Anguish : Version 20 for Windows .retrieved from https://www.worldcat.org/title/spssanalysis-without-anguish-version-20-for-windows/oclc/795333279.Creswell, J.W (2009). 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Introduction: The World‑Wide‑Web (WWW) or internet has become an important source of information including medical information globally. India stands second in the world with approximately 500 million internet users. Worldwide, about 4.5% of all internet searches are for health-related information and more than 70, 000 websites disseminate health information. Widespread use of internet as a source of health information has an effect on health-related knowledge, attitude and practices of general population as well as doctor-patient relationship. Objective: To assess the impact of health information available through internet on doctor-patient relationship from the doctor’s perspective. Methods: A web based study was conducted among the clinical doctors for the duration of 2 months. 16 questionnaires were prepared by authors and pre-validated by pilot study and expert’s opinion. Written informed consent from each 31 doctors participated in the study had taken. Result: 87% doctors agree that patient who had done internet search before the doctor's visit, takes more consultation time. 71% doctors believes that it is difficult to treat internet user patient because of distrustful behaviour and they suggest new investigations or ask for super-specialist's opinion. 90 % doctors agree that distrustful patients misuses the internet health information to test doctor's knowledge. 77% doctors believes that in spite of google search patients still have same faith on doctor. Conclusion: The impact of health information available through internet on doctor-patient relationship is both positive and negative and they perceive the overall effects on doctor-patient relationship as neutral.

Abstract The development of wireless sensing technologies paves the way for advances in the fields of wearable devices, prosthetics, and robotics. Wireless communication between sensors and readers plays an important role in recent internet of things (IoT) technologies. Among many types of wireless sensing devices, wireless passive radio frequency (RF) devices including LC (inductor-capacitor) resonators have been spotlighted. However, passive LC sensors suffer from short-range wireless detection, and their fabrication process requires several processes. Here, we designed a 3D integrated wireless compact LC location sensor fabricated by the 3D printing method for the multi-layered devices. The fabricated wireless sensing system shows the increased wireless reading distance up to ten centimeters. Also, a dielectric material with high dielectric permittivity has been applied to enhance the quality factor of the sensors by 2.5 times with improved wireless detection.

There are several problems that continue to plague the Philippine health care system. The cost of branded drugs in the Philippines is 22 times more than international reference prices while generic drugs are 4 times more.1 Despite price reductions due to legislations such as the Cheaper Medicines Act of 2008, as well as the Generics Act of 1988, those in the lower-income brackets still cannot afford maintenance medicines for hypertension and diabetes as well as antibiotics.2 Access to medicines and care from physicians and other healthcare professionals is especially challenging for geographically isolated and disadvantaged areas wherein the people are physically or economically inaccessible. Filipino traditional medicine, of which herbal medicine plays a large role has been around for centuries and is wellaccepted in the rural areas. Validating the use of these traditional medicinal plants through research is essential in order to have an evidence-based practice of herbal medicine. The main areas of research can be defined as (1) herbal medicine quality and standardization, (2) preclinical pharmacological assessments and action mechanisms, and (3) clinical efficacy and safety assessments.3 These types of researches aimed at developing safe and efficacious, as well as low-cost Philippine herbal medicines, may well be a long-term solution to the obstacles to a healthy population cited above. Our Philippine medicinal plants are a valuable but often underappreciated resource with innumerable applications for non-communicable and communicable disease indications. Limited research in this field had long been ongoing in the Philippines. Support for this movement came with the passage of the Traditional and Alternative Medicine Act of 1997 which affirmed the commitment of the government towards the support and development of traditional medicine including herbal medicine.4 Another boost was the endorsement of the Department of Health of the Sampung Halamang Gamot in the 1990s.5 The World Health Organization has advocated the integration of Traditional Medicine within national health care systems and has urged governments to develop and implement national traditional medicine policies and programs especially with Universal Health Coverage.6 It was the National Integrated Research Program of the Philippines (NIRPROMP) who was at the forefront of this field and conducted the initial studies of these Ten Medicinal Plants.7 Many of them have been developed into modern formulations. These include Lagundi (Vitex negundo) tablet and syrup for cough and asthma, Sambong (Blumea balsamifera) tablet as a diuretic and treatment of urolithiasis, Tsaang gubat (Ehretia microphylla) tablet for gastrointestinal and biliary colic, Akapulco (Senna alata) lotion for cutaneous fungal infections, Yerba Buena (Mentha villosa) tablet as an analgesic, Ulasimang bato (Peperomia pellucida) tablet for the treatment of gout and hyperuricemia. Ampalaya (Momordica charantia) tablet was also developed as a glucose-lowering agent, but it is presently undergoing researches on the reformulated tablet.8 Several of the articles in this issue present evidence for the use of some of the mentioned medicinal plants. The NIRPROMP was the forerunner and is still an integral part of the Institute of Herbal Medicine. Lagundi and Sambong have been integrated into the clinical practice of physicians in the Philippines, even by specialists. They have both been scientific and commercial successes.9 Their production has contributed to the revenue and growth of the Philippine Pharmaceutical Industry, as well as improved the economic status of farmers cultivating these crops. Developing more herbal medicines needed for primary health care would decrease our dependence on the importation of medicines, and increase the accessibility of drugs even in geographically isolated areas. Bringing back the herbal medicine gardens to the barangays in the rural communities will also assist in empowering the population. The integration of herbal medicines into mainstream clinical practice will only be possible if the researches performed, both non-clinical and clinical, are as robust as those for synthetic medicine. Cecilia C. Maramba-Lazarte, MD Director Institute of Herbal Medicine National Institutes of Health University of the Philippines Manila REFERENCES 1. Paris J. Pharma Companies Offer to Cut Drug Prices [Internet] Rappler. 2019 25 October [cited 2020 Jan 15]. Available from https://www. rappler.com/nation/243372-pharmaceutical-companies-offer-cut-drug-prices. 2. Clarete RL, Llanto GM. 2017. Access to medicines in the Philippines: Overcoming the barriers [Internet]. Philippine Institute for Development Studies. 2017 [cited 2020 Jan 14]. Available from http://hdl.handle.net/11540/7967. 3. Zhang AL, Xue CC, Fong HH. Integration of Herbal Medicine into Evidence-Based Clinical Practice Current Status and Issues. In: Benzie IFF, Wachtel-Galor S, eds. Herbal Medicine: Biomolecular and Clinical Aspects, 2nd ed. Boca Raton (FL): CRC Press/Taylor & Francis; 2011. 4. Traditional and Alternative Medicine Act (TAMA) of 1997, Republic Act No. 8423, Approved: December 9, 1997. 5. World Health Organization. Report of the Working Group on Herbal Medicines Meeting, March 1997. 6. World Health Organization. WHO Traditional Medicine Strategy: 2014-2023. 7. Eusebio JE, Umali BE. Inventory, documentation and status of medicinal plants research in Philippines. In: Batugal PA, Kanniah J, Young LS, Oliver JT, editors. Medicinal plants research in Asia, Volume 1: The framework and project workplans. Selangor DE, Malaysia: International Plant Genetic Resource Institute-Regional office for Asia, the Pacific and Oceania (IPGRI-APO), Serdang; 2004. 8. Purificacion J, Maramba N. Research Proposal Phase 1 Clinical Trial: Safety and Efficacy of Lyophilized Momordica charantia (Ampalaya) leaf tablet among Normal Volunteer Subjects (2018 version). 9. From Herbal Folklore to Modern Medicine [Internet]. World Intellectual Property Organization. 2013 [cited 2020 Jan 14]. Available from https://www.wipo.int/ipadvantage/en/details.jsp?id=3661.

Individual performance, as one of the productivity components, is determined mainly by appropriate knowledge and skills, high work motivation, and good health status. At the enterprise level, productivity levels depend on many factors, including market forces, company policies, seasons, or societal situations such as the pandemic we are currently facing. Health is only one factor, interacting with other factors and influencing performance and ultimately, productivity. A person in a healthy state who has physical and emotional abilities, accompanied by a desire to work, will show high performance. Individual performance measures are generally based on time measures, namely absenteeism, which is not coming to work due to health problems, and presentism, namely unproductive time at work due to health problems. High worker performance will lead to higher productivity because it can produce better goods and services, more creativity and innovation, high intellectual capacity, and reliable resilience. High productivity can lead to higher profits. Many health conditions and health risks affect the decline in performance. The Healthy Workplace framework showed a high association between health conditions and health risks with absenteeism and presentism.1 Health risks associated with absenteeism include lack of physical activity, high stress, and diabetes. Diabetes is the highest risk factor for absenteeism, which increases the chance of absenteeism by 2.3 times (OR=2.29, 95% CI 1.17, 4.47). Stress most increased presentism odds (OR=2.09; 95% CI 1.65, 2.63), followed by unmet emotional needs (OR=1.93, 95% CI 1.52, 2.44). Other health risks were associated with presentism, including poor nutrition, body mass index and low physical activity.2 Chronic diseases such as hepatic disorders are a risk factor for young mining workers.3 WHO's Global Health Estimates provide the data that seven of the top ten causes of death globally are non-communicable diseases. Ischaemic heart disease, stroke, and chronic obstructive pulmonary disease are the leading causes of death in high-income and lower-middle-income countries.4 The other study found that smoking, occupational risk, and air pollutants were high rank of COPD risk factors. These diseases generally occur in the working-age group, indicated by the prevalence of smoking, which is 31%, the prevalence of hypertension at the age of >18 years, which is 31.3% in men and 36.9% in women.5 The main nutritional problems in the working-age group based on the Indonesian Basic Health Survey 2018 were obesity (prevalence 21.8% at age >18 years), anemia (48.9% in pregnant women), and chronic energy deficiency (32%). The other health problems in this working age group were mental-emotional disorders (9.8%) with an increase compared to 6% in the Basic Health Survey 2013.5 The prevalence of anemia and chronic energy deficiency is still high in women of childbearing age who are also included in the productive age group.6,7 With a high participation rate of female workers and a high prevalence of chronic energy deficiency and anemia, nutritional problems are immense. In low-income countries, Infectious diseases, especially tuberculosis (TB) and HIV/AIDS, are also a health problem in the working-age population.4 Tuberculosis has a considerable impact on families. Studies in Indonesia show that although treatment is guaranteed through the National Health Insurance, households affected by TB still face the risk of poverty. Impoverishment occurs due to additional expenses outside of treatment for treatment, including reduced income due to not coming to work.8 The effect of health problem on worker productivity is getting bigger, along with the increasing risk of work accidents. The cost of per work accidents case in Europe is high; highest for The Netherlands (€ 73,410) and the lowest for Poland (€ 37,860). The total costs ranged from 2.9% of gross domestic product (GDP) for Finland to 10.2% for Poland. The cost calculation considered three categories: direct healthcare, indirect productivity, and intangible health-related quality of life costs.9 The most significant work accidents came from the construction and manufacturing sectors (32%), followed by the transportation sector (9%), forestry (4%), and mining (2%). Unfortunately, there is no exact data on the magnitude of occupational diseases. Indirect costs related to lost workdays, costs for absenteeism payments, and worker presentism were considerable. In addition, the burden of personal expenses incurred by workers for transportation costs and other costs to obtain health services decreases productivity. Medical, social and economic consequences of non-communicable diseases, occupational accidents and diseases, and nutritional problems lead to public health problems in the working population. The workplace-based intervention to overcome the workers' health problem by integrating occupational health services is a method of choice.10 Since 2007, the World Health Organization (WHO) has launched Workers' Health: A Global Plan of Action. The action plan responds to the importance of workers' health as a prerequisite for productivity and economic development through increasing access to health services for workers and building a healthy workplace.11 Furthermore, WHO introduced the Healthy Workplace Framework as practical guidance. A healthy workplace is a place where workers and managers work together continuously to make improvements to protect and promote the health, safety, and welfare of workers and workplace sustainability. There are four interrelated factors: a) health and safety in the physical environment, b) health, safety, and wellbeing in the psychosocial environment, including workplace organization and work culture, c) health resources in the workplace; and d) workplace community participation to improve the health of workers, their families, and the surroundings.1 The implementation of the healthy workplace program needs supporting evidence on workplace-based intervention through relevant research. This is what this special issue on Occupational Health of the Acta Medica Philippina is facilitating. Prof. Muchtaruddin Mansyur, MD, PhDDepartment of Community Medicine Faculty of Medicine Universitas Indonesia REFERENCES Burton J. WHO Healthy Workplace Framework and Model: Background and Supporting Literature and Practices. Geneva: World Health Organisation; 2010. p. 123. Available from: https://www.who.int/occupational_health/WHO_health_assembly_en_web.pdf Boles M, Pelletier B, Lynch W. The relationship between health risks and work productivity. J Occup Environ Med. [Internet]. [cited 2021 Aug 24]. Available from: doi: 10.1097/01.jom.0000131830.45744.97 PMID: 15247814. Mansyur M, Jen Fuk L. Mining workers, administrative task, obesity, hypertriglyceridemia, and young workers increased risk liver function elevation among Indonesian male workers April 201875(Suppl 2):A244.2-A244 DOI: 10.1136/oemed-2018-ICOHabstracts.697. BMJ Occup Environ Med [Internet]. [cited 2021 Aug 24]. 2018;75(Suppl 2):224. Available from: https://oem.bmj.com/content/75/Suppl_2/A244.2 World Health Organisation. Top Ten Cause of Death 15 August 2007 [Internet]. [cited 2021 Aug 24], Geneva: World Health Organisation. Available from: https://www.who.int/news-room/fact-sheets/detail/the-top-10-causes-of-death Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan. Hasil Utama Riskesdas 2018 [Internet]. [cited 2021 Aug 24]. Kementerian Kesehatan RI; 2019. 220 p. Available from: https://kesmas.kemkes.go.id/assets/upload/dir_519d41d8cd98f00/files/Hasil-riskesdas-2018_1274.pdf Reskia RN, Hadju V, Indriasari R, Muis M. Anemia, chronic energy deficiency and their relationship in preconception women. Enfermería Clínica. 2020; 30 (Suppl. 6):76-80 Msemo OA, Bygbjerg IC, Møller SL, Nielsen BB, Ødum L, Perslev K, et al. (2018) Prevalence and risk factors of preconception anemia: a community-based cross-sectional study of rural women of reproductive age in Northeastern Tanzania. PLoS ONE 13(12): e0208413. doi:10.1371/journal.pone.0208413 Fuady A, Houweling TAJ, Mansyur M, Richardus JH. Catastrophic total costs in tuberculosis-affected households and their determinants since Indonesia’s implementation of universal health coverage. Infect Dis Poverty. 2018; 7(1). Tompa E, Mofidi A, van den Heuvel S, van Bree T, Michaelsen F, Jung Y, et al. Economic burden of work injuries and diseases: a framework and application in five European Union countries. BMC Public Health. 2021; 21(49). doi:10.1186/s12889-020-10050-7 Mansyur M, Khoe LC, Karman MM, Ilyas M. Improving workplace-based intervention in Indonesia to prevent and control anemia. J Prim Care Community Heal. 2019; 10. World Health Organization. Workers’ health: global plan of action. In: Sixtieth World Health Assembly Agenda. 23 May 2007 [Internet]. [cited 2021 Aug 24]. Geneva: World Health Organisation; 2007. p. Available from: https://www.who.int/occupational_health/WHO_health_assembly_en_web.pdf

Over the last decade or so, much has been written about the possibilities offered by the internet for creating sites of community based on exchange, collaboration, and reciprocity. Since Howard Rheingold published his polemic, The Virtual Community, in 1993, much has been written on this subject. The notion of just what constitutes ‘virtual reality’ has been extensively debated; however, ‘community’ is almost universally assumed to be good. There are failed communities and successful communities, but the critique of ‘community’ itself as a concept stops there. How, then, do we account for websites that create a sense of community precisely through the promotion of hatred and violence, and on which hatred of others is what the community ‘has in common’? Community as Good: The Origins of Virtual Community The term ‘community’ suggests communication; indeed, the work derives from the Latin communicare, which, as Peter Gould explains, ‘originally meant to share, to join and to unite” (3), and from which is also derived the verb ‘to communicate.’ Hence, accounts of online culture draw on this definition of community, suggesting that computer technology brings people together by allowing them to communicate. Such proximity is, therefore, privileged over geophysical location. In recent debates about cyber-culture, definitions of online community tend to define community through the concept of ‘shared interests’. What is more, some accounts of cyber-culture share a certain view of online community as inherently liberating. The Bad Community: God Hates Fags God Hates Fags is perhaps one of the best-known far-right sites on the Web. It is a non-interactive website, set up and maintained by Benjamin Phelps, pastor of Westboro Baptist Church in Topeka, Kansas, in association with his grandfather Fred Phelps, who originally founded the church in 1964. Phelps first achieved notoriety in 1991 when he organised a picket of the San Francisco Pride Parade, to ‘warn this evil city that they’re going the way of Sodom’. In 1997, the church’s members were ordered by the American Supreme Court to limit their picketing activities after they targeted a local Episcopalian church that they claimed had promoted gay rights. Since the ruling, church members have continued their campaign of homophobic picketing. However, it is as an online promoter of homophobia and other forms of hatred that Phelps has achieved notoriety on an international scale. On paper, Westboro Baptist Church’s Website seems like the perfect example of the Net’s utility as a means of giving voice to small, marginalised community groups, and of bringing together people who share ‘a commonality of interests and goals’. However, this, like other Christian fundamentalist sites, challenges the view of such networks as essentially liberating (though they are certainly utopian in tone), since their shared interests happen to include insisting that creationist dogma be taught in schools, picketing the funerals of those who die of Aids or as a result of homophobic attacks, and promoting violence against lesbians and gay men. God Hates Fags sees itself as both a site of community and as a pressure group fighting a desperately immoral liberal society. It also draws on the idea of a society becoming good through the erasure of certain marginalised subjects, with the erasure to take the form of individuals suppressing their sexual identity in real life, not just online. While God Hates Fags and other sites like it primarily express the fantasy of a post-apocalyptic New Jerusalem. They do so by referring to fantasies of the nation (as a space that must be purified in order for this apocalyptic transformation to take place), of the online community (here imagined as a community of haters), and of the local community producing the site (who, far from being a small, marginal force, are re-presented as a community of ‘knowers’ attempting to promote ‘the truth’ about life in the United States: that is, as a force for good). Fantastic communities are often unaware of their own violence, and the community that hates is no exception, although its claims to peacefulness often stretch credulity to a greater than usual extent. Here is Westboro’s description of its ‘peaceful’ protests: WBC engages in daily peaceful sidewalk demonstrations opposing the homosexual lifestyle of soul-damning, nation-destroying filth. We display large, colourful signs containing Bible words and sentiments, including: GOD HATES FAGS, FAGS HATE GOD, AIDS CURES FAGS, THANK GOD FOR AIDS, FAGS BURN IN HELL, GOD IS NOT MOCKED, FAGS ARE NATURE FREAKS [sic] … FAGS DOOM NATIONS, etc. (God Hates Fags) The site’s authors are able to claim such sentiments as ‘non-violent’ precisely because of the way that violence is imagined purely in terms of the physical act; that is, as embodied. Discursive violence, the violence of the text, is not recognised as such. Reading the passages above, I find it hard to maintain any sense of critical distance at the notion of picketing a funeral, and then going online to publicise the activity and exhort others to do the same. The site is frustrating precisely because it assumes the reader’s sympathy. For Phelps, a community of ‘fag haters’ already exists within the wider, corrupt national community of the United States; the site merely serves to unite this community and to provide it with resources. Nevertheless, the statement is itself part of the process by which the site attempts to construct a community through a process of rehabilitation, which aims to re-position hatred of homosexuals both as a political position and as an identity position. The site assumes that the experience of hatred, like that of other extreme emotions, has been wrongly constructed as essentially private, even impossible to articulate. Phelps assures us that it is not, that our hatred (and the reader is always assumed to be on side; the site is never defensive in tone, and never attempts to address its critics) is shared by others. The community exists in the bodies of individuals; by making hatred public and visible, the community can finally become visible in the public domain. This site, and others like it, provide a chilling new perspective on the notion of ‘shared interests’ as a basis for community, as well as giving an insight into the ways in which inequalities might not only translate from geophysical into online communities but actually be heightened, not least by the liberal rhetoric of free speech in which the intended victims of such assaults are urged simply to ignore them, even as they are imposed an ever-increasing number of victims (Porter 234-5). In order to justify their attacks on outsiders, hate sites reproduce discourses of virtual community alongside fundamentalist dogma. So, for example, Westboro Baptist Church claims that it is necessary to draw together a community based on a shared homophobic response in order to protect the larger community of the nation from destruction. In order to construct the virtual community then, it is necessary to mobilise fantasies of the nation as it might be in an ideal world. The community does not simply represent the wider community of the United States; that is, it is not a ‘virtual America.’ Rather, it draws upon a fantasy of the nation as perfectible, and this fantasy assumes a desire to purify the nation by destroying or expelling strangers. Despite the dystopian violence of Phelps’s vision, however, I do not think it is enough to argue that such manifestations are simply an example of a medium with great potential for spiritual growth falling into the wrong hands. Margaret Wertheim seems to predict the use of the Internet to promote hatred when she writes that ‘[t]here is every potential, if we are not careful, for cyberspace to be less like Heaven, and more like Hell’ (298). This reading of virtual culture tends to normalise the idea of a utopian internet community, from which deviations occur only as the result of insufficient vigilance. What is more, the invocation of a group of right-thinking cyber-citizens—the ‘we’ who must be ‘careful’—reproduces the very liberal rhetoric which, as I have argued, tends to perpetuate, or at least obscure, power structures within online communities. Indeed, the notion of ‘the online community’ invoked here seems, ironically, to reproduce the notion of a single unlimited community which, if it is not conterminous with all mankind exactly, is certainly conterminous with all (responsible) users of the internet. As I have shown, it is by drawing on the notions of universality and redemption that underpin utopian theories of cyber-culture that Phelps is able to present his site as a site of community. I would suggest, then, that the notion of a community that has the potential to be good but is constantly under threat from deviant outsiders, is inadequate. Rather, it is necessary to pay attention to the ways in which utopian rhetoric might in itself play a role in reproducing inequalities that exist in society more generally, both online and off. References Gould, P. “Dynamic Structures of Geographic Space.” Collapsing Space and Time: Geographic Aspects of Communications and Information. Eds. S.D. Brunn and T.R. Leinbach. London: HarperCollins, 1991. 3-30. Porter, J.E. “Liberal Individualism and Internet Policy: A Communitarian Critique.” Passions, Pedagogies, and 21st-Century Technologies. Eds. G.E. Hawisher and C.L. Selfe. Logan: Utah State UP, 1999. Rheingold, H. The Virtual Community: Homesteading on the Electronic Frontier. HarperPerennial, 1993. 16 Oct. 2002 http://www.well.com/www/hlr/vcbook/index.html>. Wertheim, M. The Pearly Gates of Cyberspace: A History of Space from Dante to the Internet. London: Virago Press, 1999. Citation reference for this article MLA Style Ferreday, Debra. "Bad Communities: Virtual Community and Hate Speech." M/C Journal 8.1 (2005). echo date('d M. Y'); ?> <http://journal.media-culture.org.au/0502/07-ferreday.php>. APA Style Ferreday, D. (Feb. 2005) "Bad Communities: Virtual Community and Hate Speech," M/C Journal, 8(1). Retrieved echo date('d M. Y'); ?> from <http://journal.media-culture.org.au/0502/07-ferreday.php>.

Occupational health and nutrition are closely related. Nutrition in the workplace plays an essential role in supporting workers' health through a balanced nutrition approach. Reciprocally Occupational health is an approach to solving nutritional problems in workers, which in turn impacts the nutrition and health of families and communities. The workplace-based nutrition promotion is one of the pillars of the occupational health approach to solving health problems among workers. The workers' health related to nutrition is still a big challenge; on the other hand, the workplace is a potential setting for solving workers' and community health and nutrition problems. Currently, the Southeast Asian Region has been facing multiple burdens of the workers/productive age nutrition problem. The problem of nutritional deficiency, especially iron and other nutrients deficiency anemia dominate in women workers, especially pregnant women. The prevalence of anemia in working women is high in some Asian countries such as Bangladesh, Cambodia, India, Indonesia, Maldives, Myanmar, Nepal, and Timor Leste. The prevalence of anemia in women of productive age in these countries ranges from 22.7% to more than 50%.1 Meanwhile, other nutritional deficiencies such as folic acid and zinc also add to the magnitude of the calorie and protein deficiency problem in this population. Along with the problem of nutritional deficiency, Southeast Asian countries are also facing obesity and the risk of non-communicable diseases related to diets, such as hypertension and cardiovascular disease. It was noted that the prevalence of obesity in workers reached 49.8% among males and 50.2% among females.2 Work and work environment are significant risk factors for obesity among workers. Social stressors, psychosocial work factors, working hours, sleep and night shift work, and sedentary behavior are associated with obesity among workers.3 In addition, food contamination due to the environment, including work environment, increases health risks in the workplace.4 This situation is certainly threatening because it is also a determinant variable of presenteeism and reduces productivity. Many programs have been carried out to prevent and control these health and nutrition problems, including health and nutrition promotion, supplementation of iron tablets, and food fortification. The program's success will undoubtedly be improved by making the workplace a setting for activities towards a healthy workplace. World Health Organization5 defined "a healthy workplace is one in which workers and managers collaborate to use a continualimprovement process to protect and promote the health, safety and well-being of all workers and the sustainability of the workplace".The principles of approach to workplace health promotion are addressed at the underlying causes of ill health, combining diverse methods of interventions. Workplace health promotion is aimed at effective worker participation and is not partially based on medical activities but should be part of the work organization and working conditions. These approaches are in line and build a synergy with the goals of the occupational health services that are devoted to the prevention and management of occupational and environmental injury, illness and disability, and the promotion of health and productivity of workers, their families, and communities. In practice, the goals of the occupational health service are to protect the workers against any occupational hazard, to contribute towards harmonious human-machine relationships and to establish and maintain the workers' physical and mental well-being.6 The bottom line of workplace-based health and nutrition intervention is the worker's healthy behavior and balanced nutrition practices. Workplace health promotion should be done by implementing health promotion strategies focusing on the individual and their lifestyle behavior change and creating healthy work organizations and environments. In the case of nutritional deficiencies, improving diets may be more appropriate, and workplaces are an excellent setting to improve the nutritional quality of diets. Nutrition promotion is the basis of the strategic approach to address nutrient deficiency anemia. Workplaces appear as the main entry point: workers are generally healthy and productive, and nutrition activities may also reach the workers' families. Mansyur7 proposed integrating nutrition education, factory canteen and health services improvements. Workplace-based nutrition education effectively improves female workers' knowledge and practices of balanced nutrition and healthy habits. The service of the company canteen plays an important role in providing nourishing meals and compensating for family food shortages due to financial constraints and cultural barriers. In addition, factory health services strengthen their activities with health promotion and preventive approach via workplace hazard prevention and control, and minimizing the risk of occupational and environmental food contaminants. Muchtaruddin Mansyur, MD, MSc, PhDSouth East Asian Ministers of Education OrganizationRegional Center for Food and NutritionDepartment of Community Medicine, Faculty of Medicine,Universitas IndonesiaJakarta, Indonesia REFERENCES1. Aziz Ali S, Abbasi Z, Feroz A, Hambidge KM, Krebs NF, WestcottJE, et al. Factors associated with anemia among women of thereproductive age group in Thatta district: study protocol. ReprodHealth. 2019 Mar;16(1):34. doi: 10.1186/s12978-019-0688-7.2. Ni Ketut Putri SM, Meo CM, Saverinus S, Halimatunnisa M, SusantiI. Factors associated with obesity in adults in South East Asia. IntJ Psych Rehab. 2020 Jul; 24(7):7599 -7607.3. Yarborough CM, Brethauer S, Burton WN, Fabius RJ, Hymel P,Kothari S, et al. Obesity in the workplace: Impact, outcomes, andrecommendations. J Occup Environ Med. 2018 Jan; 60(1):97-107.doi:10.1097/JOM.00000000000012204. Ross P, Kemerer S, Taylor L. Food safety in the workplace: apractical approach. AAOHN J. 2006 Dec; 54(12): 521-530. doi:10.1177/216507990605401203.5. Burton J. WHO Healthy Workplace Framework and Model:Background and Supporting Literature and Practices [Internet].Geneva, World Health Organization. 2010 Feb [cited 2022 Jan 9].Available from: https://www.who.int/occupational_health/healthy_workplace_framework.pdf.6. World Health Organization. Workers' health: global plan of action. In:Sixtieth World Health Assembly Agenda. [Internet]. Geneva: WorldHealth Organization. 2007 May 23 [ cited 2022 Jan 9]. Availablefrom: https://www.who.int/occupational_health/WHO_health_assembly_en_web.pdf.7. Mansyur M, Khoe LC, Karman MM, Ilyas M. Improvingworkplace-based intervention in Indonesia to prevent and controlanemia. J Prim Care Community Health. 2019 Jan-Dec; 10:2150132719854917. doi:10.1177/2150132719854917

This review provides a rough sketch of the evidence, gaps and promising directions in e-learning from 2000 onwards, with a particular focus on Canada. We searched a wide range of sources and document types to ensure that we represented, comprehensively, the arguments surrounding e-learning. Overall, there were 2,042 entries in our database, of which we reviewed 1,146, including all the Canadian primary research and all scholarly reviews of the literature. In total, there were 726 documents included in our review: 235 – general public opinion; 131 – trade/practitioners’ opinion; 88 – policy documents; 120 – reviews; and 152 – primary empirical research. The Argument Catalogue codebook included the following eleven classes of variables: 1) Document Source; 2) Areas/Themes of e-learning; 3) Value/Impact; 4) Type of evidence; 5) Research design; 6) Area of applicability; 7) Pedagogical implementation factors; 8) A-priori attitudes; 9) Types of learners; 10) Context; and 11) Technology Factors. We examined the data from a number of perspectives, including their quality as evidence. In the primary research literature, we examined the kinds of research designs that were used. We found that over half of the studies conducted in Canada are qualitative in nature, while the rest are split in half between surveys and quantitative studies (correlational and experimental). When we looked at the nature of the research designs, we found that 51% are qualitative case studies and 15.8% are experimental or quasi-experimental studies. It seems that studies that can help us understand “what works” in e-learning settings are underrepresented in the Canadian research literature. The documents were coded to provide data on outcomes of e-learning (we also refer to them as “impacts” of e-learning). Outcomes/impacts are the perceived or measured benefits of e-learning, whereas predictors are the conditions or features of e-learning that can potentially affect the outcomes/impacts. The impacts were coded on a positive to negative scale and included: 1) achievement; 2) motivation/satisfaction; 3) interactivity/ communication; 4) meeting social demands; 5) retention/attrition; 6) learning flexibility; and 7) cost. Based on an analysis of the correlations among these impacts, we subsequently collapsed them (all but cost) into a single impact scale ranging from –1 to +1. We found, generally, that the perception of impact or actual measured impact varies across the types of documents. They appear to be lower in general opinion documents, practitioner documents and policy making reports than in scholarly reviews and primary research. While this represents an expression of hope for positive impact, on the one hand, it possibly represents reality, on the other. Where there were sufficient documents to examine and code, impact was high across each of the CCL Theme Areas. Health and Learning was the highest, with a mean of 0.80 and Elementary/Secondary was the lowest, with a mean of 0.77. However, there was no significant difference between these means. The impact of e-learning and technology use was highest in distance education, where its presence is required (Mean = 0.80) and lowest in face-to-face instructional settings (Mean = 0.60) where its presence is not required. Network-based technologies (e.g., Internet, Web-based, CMC) produced a higher impact score (Mean = 0.72) than straight technology integration in educational settings (Mean = 0.66), although this difference was considered negligible. Interestingly, among the Pedagogical Uses of Technology, student applications (i.e., students using technology) and communication applications (both Mean = 0.78) had a higher impact score than instructional or informative uses (Mean = 0.63). This result suggests that the student manipulation of technology in achieving educational goals is preferable to teacher manipulation of technology. In terms of predictor variables (professional training, course design, infrastructure/ logistics, type of learners [general population, special needs, gifted], gender issues and ethnicity/race/religion/aboriginal, location, school setting, context of technology use, type of tool used and pedagogical function of technology) we found the following: professional development was underrepresented compared to issues of course design and infrastructure/ logistics; most attention is devoted to general population students, with little representation of special needs, the gifted students, issues of gender or ethnic/race/religious/aboriginal status; the greatest attention is paid to technology use in distance education and the least attention paid to the newly emerging area of hybrid/blended learning; the most attention is paid to networked technologies such as the Internet, the WWW and CMC and the least paid to virtual reality and simulations. Using technology for instruction and using technology for communication are the two highest categories of pedagogical use. In the final stage, the primary e-learning studies from the Canadian context that could be summarized quantitatively were identified. We examined 152 studies and found a total of 7 that were truly experimental (i.e., random assignment with treatment and control groups) and 10 that were quasi-experimental (i.e., not randomized but possessing a pretest and a posttest). For these studies we extracted 29 effect sizes or standardized mean differences, which were included in the composite measure. The mean effect size was +0.117, a small positive effect. Approximately 54% of the e-learning participants performed at or above the mean of the control participants (50 th percentile), an advantage of 4%. However, the heterogeneity analysis was significant, indicating that the effect sizes were widely dispersed. It is clearly not the case that e-learning is always the superior condition for educational impact. Overall, we know that research in e-learning has not been a Canadian priority; the culture of educational technology research, as distinct from development, has not taken on great import. In addition, there appears to have been a disproportionate emphasis on qualitative research in the Canadian e-learning research culture. We noted that there are gaps in areas of research related to early childhood education and adult education. Finally, we believe that more emphasis must be placed on implementing longitudinal research, whether qualitative or quantitative (preferably a mixture of the two), and that all development efforts be accompanied by strong evaluation components that focus on learning impact. It is a shame to attempt innovation and not be able to tell why it works or doesn’t work. In this sense, the finest laboratories for e-learning research are the institutions in which it is being applied. Implications for K-12 Practitioners When implemented appropriately, technology tools are beneficial to students’ learning, and may facilitate the development of higher order thinking skills. Student manipulation of technology in achieving the goals of education is preferable to teacher manipulation of technology. Teachers need to be aware of differences between instructional design for e-learning as compared to traditional face-to-face situations. Immediate, extensive, and sustained support should be offered to teachers in order to make the best out of e-learning. Implications for Post-Secondary Some educators suggest that e-learning has the potential to transform learning, but there is limited empirical research to assess the benefits. Post-secondary education would benefit from a Pan-Canadian plan to assess the impact of e-learning initiatives. It is important that instructional design match the goals and potential of e-learning. Research is needed to determine the feasibility and effectiveness of such things as learning objects and multimedia applications. Properly implemented computer mediated communication can enrich the learning environment; help reduce low motivation and feelings of isolation in distance learners. E-learning appears to be more effective in distance education, where technology use is required than in face-to-face instructional settings. Implications for Policy Makers Effective and efficient implementation of e-learning technologies represents new, and difficult, challenges to practitioners, researchers, and policymakers. The term e-learning has been used to describe many different applications of technology, which may be implemented in a wide variety of ways (some of which are much more beneficial than others). School administrators must balance the needs of all stakeholders, and the cost-benefit ratios of technology tools, in deciding not only which technologies to use, but also when and how to implement new technologies. Traditional methods of instructional design and school administration must be adjusted to deal with the demands of distance education and other contexts of technology use. Professional education, development, and training for educators must ensure that teachers will be equipped to make optimal pedagogical use of new methods.

Internet Relay Chat (IRC) is a text-based computer-mediated communication (CMC) service in which people can meet and chat in real time. Most chat occurs in channels named for a specific topic, such as #usa or #linux. A user can take part in several channels when connected to an IRC network. For a long time the only major IRC network available was EFnet, founded in 1990. Over the 1990s three other major IRC networks developed, Undernet (1993), DALnet (1994) and IRCnet (which split from EFnet in June 1996). Several causes led to the separate development of IRC networks: fast growth of user numbers, poor scalability of the IRC protocol and content disagreements, like allowing or prohibiting 'bot programs. Today we are experiencing the development of regional IRC networks, such as BrasNet for Brazilian users, and increasing regionalisation of the global networks -- IRCnet users are generally European, EFnet users generally from the Americas and Australia. All persons connecting to an IRC network at one time create that IRC network's user space. People are constantly signing on and off each network. The total number of users who have ever been to a specific IRC network could be called its 'social space' and an IRC network's social space is by far larger than its user space at any one time. Although there has been research on IRC almost from its beginning (it was developed in 1988, and the first research was made available in late 1991 (Reid)), resources on quantitative development are rare. To rectify this situation, a quantitative data logging 'bot program -- Socip -- was created and set to run on various IRC networks. Socip has been running for almost two years on several IRC networks, giving Internet researchers empirical data of the quantitative development of IRC. Methodology Any approach to gathering quantitative data on IRC needs to fulfil the following tasks: Store the number of users that are on an IRC network at a given time, e.g. every five minutes; Store the number of channels; and, Store the number of servers. It is possible to get this information using the '/lusers' command on an IRC-II client, entered by hand. This approach yields results as in Table 1. Table 1: Number of IRC users on January 31st, 1995 Date Time Users Invisible Servers Channels 31.01.95 10:57 2737 2026 93 1637 During the first months of 1995, it was even possible to get all user information using the '/who **' command. However, on current major IRC networks with greater than 50000 users this method is denied by the IRC Server program, which terminates the connection because it is too slow to accept that amount of data. Added to this problem is the fact that collecting these data manually is an exhausting and repetitive task, better suited to automation. Three approaches to automation were attempted in the development process. The 'Eggdrop' approach The 'Eggdrop' 'bot is one of the best-known IRC 'bot programs. Once programmed, 'bots can act autonomously on an IRC network, and Eggdrop was considered particularly convenient because customised modules could be easily installed. However, testing showed that the Eggdrop 'bot was unsuitable for two reasons. The first was technical: for reasons undetermined, all Eggdrop modules created extensive CPU usage, making it impossible to run several Eggdrops simultaneously to research a number of IRC networks. The second reason had to do with the statistics to be obtained. The objective was to get a snapshot of current IRC users and IRC channel use every five minutes, written into an ASCII file. It was impossible to extend Eggdrop's possibilities in a way that it would periodically submit the '/lusers' command and write the received data into a file. For these reasons, and some security concerns, the Eggdrop approach was abandoned. IrcII was a UNIX IRC client with its own scripting language, making it possible to write command files which periodically submit the '/lusers' command to any chosen IRC server and log the command's output. Four different scripts were used to monitor IRCnet, EFnet, DALnet and Undernet from January to October 1998. These scripts were named Socius_D, Socius_E, Socius_I and Socius_U (depending on the network). Every hour each script stored the number of users and channels in a logfile (examinable using another script written in the Perl language). There were some drawbacks to the ircII script approach. While the need for a terminal to run on could be avoided using the 'screen' package -- making it possible to start ircII, run the scripts, detach, and log off again -- it was impossible to restart ircII and the scripts using an automatic task-scheduler. Thus periodic manual checks were required to find out if the scripts were still running and restart them if needed (e.g. if the server connection was lost). These checks showed that at least one script would not be running after 10 hours. Additional disadvantages were the lengthy log files and the necessity of providing a second program to extract the log file data and write it into a second file from which meaningful graphs could be created. The failure of the Eggdrop and ircII scripting approaches lead to the solution still in use today. Perl script-only approach Perl is a powerful script language for handling file-oriented data when speed is not extremely important. Its version 5 flavour allows a lot of modules to use it for expansion, including the Net::IRC package. The object-oriented Perl interface enables Perl scripts to connect to an IRC server, and use the basic IRC commands. The Socip.pl program includes all server definitions needed to create connections. Socip is currently monitoring ten major IRC networks, including DALnet, EFnet, IRCnet, the Microsoft Network, Talkcity, Undernet and Galaxynet. When run, "Social science IRC program" selects a nickname from its list corresponding to the network -- For EFnet, the first nickname used is Socip_E1. It then functions somewhat like a 'bot. Using that nickname, Socip tries to create an IRC connection to a server of the given network. If there is no failure, handlers are set up which take care of proper reactions to IRC server messages (such as Ping-pong, message output and reply). Socip then joins the channel #hose (the name has no special meaning), a maintenance channel with the additional effect of real persons meeting the 'bot and trying to interact with it every now and then. Those interactions are logged too. Sitting in that channel, the script sleeps periodically and checks if a certain time span has passed (the default is five minutes). After that, the '/lusers' command's output is stored in a data file for each IRC network and the IRC network's RRD (Round Robin database) file is updated. This database, which is organised chronologically, offers great detail for recent events and more condensed information for older events. User and channel information younger than 10 days is stored in five-minute detail. If older than two years, the same information is automatically averaged and stored in a per-day resolution. In case of network problems, Socip acts as necessary. For example, it recognises a connection termination and tries to reconnect after pausing by using the next nickname on the list. This prevents nickname collision problems. If the IRC server does not respond to '/luser' commands three times in a row, the next server on the list is accessed. Special (crontab-invoked) scripts take care of restarting Socip when necessary, as in termination of script because of network problems, IRC operator kill or power failure. After a reboot all scripts are automatically restarted. All monitoring is done on a Linux machine (Pentium 120, 32 MB, Debian Linux 2.1) which is up all the time. Processor load is not extensive, and this machine also acts as the Sociology Department's WWW-Server. Graphs creation Graphs can be created from the data in Socip's RRD files. This task is done using the MRTG (multi router traffic grapher) program by Tobias Oetiker. A script updates all IRC graphs four times a day. Usage of each IRC network is visualised through five graphs: Daily, Weekly and Monthly users and channels, accompanied by two graphs showing all known data users/channels and servers. All this information is continuously published on the World Wide Web at http://www.hinner.com/ircstat. Figures The following samples demonstrate what information can be produced by Socip. As already mentioned, graphs of all monitored networks are updated four times a day, with five graphs for each IRC network. Figure 1 shows the rise of EFnet users from about 40000 in November 1998 to 65000 in July 2000. Sampled data is oscillating around an average amount, which is resulting from the different time zones of users. Fig. 1: EFnet - Users and Channels since November 1998 Figure 2 illustrates the decrease of interconnected EFnet servers over the years. Each server is now handling more and more users. Reasons for taking IRC servers off the net are security concerns (attacks on the server by malicious persons), new payment schemes, maintenance and cost effort. Fig. 2: EFnet - Servers since November 1998 A nice example of a heavily changing weekly graph is Figure 3, which shows peaks shortly before 6pm CEST and almost no users shortly after midnight. Fig. 3: Galaxynet: Weekly Graph (July, 15th-22nd, 2000) The daily graph portrays usage variations with even more detail. Figure 4 is taken from Undernet user and channel data. The vertical gap in the graph indicates missing data, caused either by a net split or other network problems. Fig. 4: Undernet: Daily Graph: July, 22nd, 2000 The final example (Figure 5) shows a weekly graph of the Webchat (http://www.webchat.org) network. It can be seen that every day the user count varies from 5000 to nearly 20000, and that channel numbers fluctuate in concert accordingly from 2500 to 5000. Fig. 5: Webchat: Monthly graph, Week 24-29, 2000 Not every IRC user is connected all the time to an IRC network. This figure may have increased lately with more and more flatrates and cheap Internet access offers, but in general most users will sign off the network after some time. This is why IRC is a very dynamic society, with its membership constantly in flux. Maximum user counts only give the highest number of members who were simultaneously online at some point, and one could only guess at the number of total users of the network -- that is, including those who are using that IRC service but are not signed on at that time. To answer these questions, more thorough investigation is necessary. Then inflows and outflows might be more readily estimated. Table 2 shows the all time maximum user counts of seven IRC networks, compared to the average numbers of IRC users of the four major IRC networks during the third quarter 1998 (based on available data). Table 2: Maximum user counts of selected IRC networks DALnet EFnet Galaxy Net IRCnet MS Chat Undernet Webchat Max. 2000 64276 64309 15253 65340 17392 60210 19793 3rd Q. 1998 21000 37000 n/a 24500 n/a 24000 n/a Compared with the 200-300 users in 1991 and the 7000 IRC-chatters in 1994, the recent growth is certainly extraordinary: it adds up to a total of 306573 users across all monitored networks. It can be expected that the 500000 IRC user threshold will be passed some time during the year 2001. As a final remark, it should be said that obviously Web-based chat systems will be more and more common in the future. These chat services do not use standard IRC protocols, and will be very hard to monitor. Given that these systems are already quite popular, the actual number of chat users in the world could have already passed the half million landmark. References Reid, Elizabeth. "Electropolis: Communications and Community on Internet Relay Chat." Unpublished Honours Dissertation. U of Melbourne, 1991. The Socip program can be obtained at no cost from http://www.hinner.com. Most IRC networks can be accessed with the original Net::Irc Perl extension, but for some special cases (e.g. Talkcity) an extended version is needed, which can also be found there. Citation reference for this article MLA style: Kajetan Hinner. "Statistics of Major IRC Networks: Methods and Summary of User Count." M/C: A Journal of Media and Culture 3.4 (2000). [your date of access] <http://www.api-network.com/mc/0008/count.php>. Chicago style: Kajetan Hinner, "Statistics of Major IRC Networks: Methods and Summary of User Count," M/C: A Journal of Media and Culture 3, no. 4 (2000), <http://www.api-network.com/mc/0008/count.php> ([your date of access]). APA style: Kajetan Hinner. (2000) Statistics of major IRC networks: methods and summary of user count. M/C: A Journal of Media and Culture 3(4). <http://www.api-network.com/mc/0008/count.php> ([your date of access]).

“As the old technologies become automatic and invisible, we find ourselves more concerned with fighting or embracing what’s new”—Dennis Baron, From Pencils to Pixels: The Stage of Literacy Technologies Popular music is firmly rooted within realist practice, or what has been called the "culture of authenticity" associated with modernism. As Lawrence Grossberg notes, the accelleration of the rate of change in modern life caused, in post-war youth culture, an identity crisis or "lived contradiction" that gave rock (particularly) and popular music (generally) a peculiar position in regard to notions of authenticity. Grossberg places rock's authenticity within the "difference" it maintains from other cultural forms, and notes that its difference "can be justified aesthetically or ideologically, or in terms of the social position of the audiences, or by the economics of its production, or through the measure of its popularity or the statement of its politics" (205-6). Popular music scholars have not adequately addressed issues of authenticity and individuality. Two of the most important questions to be asked are: How is authenticity communicated in popular music? What is the site of the interpretation of authenticity? It is important to ask about sound, technology, about the attempt to understand the ideal and the image, the natural and artificial. It is these that make clear the strongest connections between popular music and contemporary culture. Popular music is a particularly appropriate site for the study of authenticity as a cultural category, for several reasons. For one thing, other media do not follow us, as aural media do, into malls, elevators, cars, planes. Nor do they wait for us, as a tape player paused and ready to play. What is important is not that music is "everywhere" but, to borrow from Vivian Sobchack, that it creates a "here" that can be transported anywhere. In fact, we are able to walk around enveloped by a personal aural environment, thanks to a Sony Walkman.1 Also, it is more difficult to shut out the aural than the visual. Closing one's ears does not entirely shut out sound. There is, additionally, the sense that sound and music are interpreted from within, that is, that they resonate through and within the body, and as such engage with one's self in a fashion that coincides with Charles Taylor's claim that the "ideal of authenticity" is an inner-directed one. It must be noted that authenticity is not, however, communicated only via music, but via text and image. Grossberg noted the "primacy of sound" in rock music, and the important link between music, visual image, and authenticity: Visual style as conceived in rock culture is usually the stage for an outrageous and self-conscious inauthenticity... . It was here -- in its visual presentation -- that rock often most explicitly manifested both an ironic resistance to the dominant culture and its sympathies with the business of entertainment ... . The demand for live performance has always expressed the desire for the visual mark (and proof) of authenticity. (208) But that relationship can also be reversed: Music and sound serve in some instances to provide the aural mark and proof of authenticity. Consider, for instance, the "tear" in the voice that Jensen identifies in Hank Williams's singing, and in that of Patsy Cline. For the latter, voicing, in this sense, was particularly important, as it meant more than a singing style, it also involved matters of self-identity, as Jensen appropriately associates with the move of country music from "hometown" to "uptown" (101). Cline's move toward a more "uptown" style involved her visual image, too. At a significant turning point in her career, Faron Young noted, Cline "left that country girl look in those western outfits behind and opted for a slicker appearance in dresses and high fashion gowns" (Jensen 101). Popular music has forged a link with visual media, and in some sense music itself has become more visual (though not necessarily less aural) the more it has engaged with industrial processes in the entertainment industry. For example, engagement with music videos and film soundtracks has made music a part of the larger convergence of mass media forms. Alongside that convergence, the use of music in visual media has come to serve as adjunct to visual symbolisation. One only need observe the increasingly commercial uses to which music is put (as in advertising, film soundtracks and music videos) to note ways in which music serves image. In the literature from a variety of disciplines, including communication, art and music, it has been argued that music videos are the visualisation of music. But in many respects the opposite is true. Music videos are the auralisation of the visual. Music serves many of the same purposes as sound does generally in visual media. One can find a strong argument for the use of sound as supplement to visual media in Silverman's and Altman's work. For Silverman, sound in cinema has largely been overlooked (pun intended) in favor of the visual image, but sound is a more effective (and perhaps necessary) element for willful suspension of disbelief. One may see this as well in the development of Dolby Surround Sound, and in increased emphasis on sound engineering among video and computer game makers, as well as the development of sub-woofers and high-fidelity speakers as computer peripherals. Another way that sound has become more closely associated with the visual is through the ongoing evolution of marketing demands within the popular music industry that increasingly rely on visual media and force image to the front. Internet technologies, particularly the WorldWideWeb (WWW), are also evidence of a merging of the visual and aural (see Hayward). The development of low-cost desktop video equipment and WWW publishing, CD-i, CD-ROM, DVD, and other technologies, has meant that visual images continue to form part of the industrial routine of the music business. The decrease in cost of many of these technologies has also led to the adoption of such routines among individual musicians, small/independent labels, and producers seeking to mimic the resources of major labels (a practice that has become considerably easier via the Internet, as it is difficult to determine capital resources solely from a WWW site). Yet there is another facet to the evolution of the link between the aural and visual. Sound has become more visual by way of its representation during its production (a representation, and process, that has largely been ignored in popular music studies). That representation has to do with the digitisation of sound, and the subsequent transformation sound and music can undergo after being digitised and portrayed on a computer screen. Once digitised, sound can be made visual in any number of ways, through traditional methods like music notation, through representation as audio waveform, by way of MIDI notation, bit streams, or through representation as shapes and colors (as in recent software applications particularly for children, like Making Music by Morton Subotnick). The impetus for these representations comes from the desire for increased control over sound (see Jones, Rock Formation) and such control seems most easily accomplished by way of computers and their concomitant visual technologies (monitors, printers). To make computers useful tools for sound recording it is necessary to employ some form of visual representation for the aural, and the flexibility of modern computers allows for new modes of predominately visual representation. Each of these connections between the aural and visual is in turn related to technology, for as audio technology develops within the entertainment industry it makes sense for synergistic development to occur with visual media technologies. Yet popular music scholars routinely analyse aural and visual media in isolation from one another. The challenge for popular music studies and music philosophy posed by visual media technologies, that they must attend to spatiality and context (both visual and aural), has not been taken up. Until such time as it is, it will be difficult, if not impossible, to engage issues of authenticity, because they will remain rootless instead of situated within the experience of music as fully sensual (in some cases even synaesthetic). Most of the traditional judgments of authenticity among music critics and many popular music scholars involve space and time, the former in terms of the movement of music across cultures and the latter in terms of history. None rely on notions of the "situatedness" of the listener or musicmaker in a particular aural, visual and historical space. Part of the reason for the lack of such an understanding arises from the very means by which popular music is created. We have become accustomed to understanding music as manipulation of sound, and so far as most modern music production is concerned such manipulation occurs as much visually as aurally, by cutting, pasting and otherwise altering audio waveforms on a computer screen. Musicians no more record music than they record fingering; they engage in sound recording. And recording engineers and producers rely less and less on sound and more on sight to determine whether a recording conforms to the demands of digital reproduction.2 Sound, particularly when joined with the visual, becomes a means to build and manipulate the environment, virtual and non-virtual (see Jones, "Sound"). Sound & Music As we construct space through sound, both in terms of audio production (e.g., the use of reverberation devices in recording studios) and in terms of everyday life (e.g., perception of aural stimuli, whether by ear or vibration in the body, from points surrounding us), we centre it within experience. Sound combines the psychological and physiological. Audio engineer George Massenburg noted that in film theaters: You couldn't utilise the full 360-degree sound space for music because there was an "exit sign" phenomena [sic]. If you had a lot of audio going on in the back, people would have a natural inclination to turn around and stare at the back of the room. (Massenburg 79-80) However, he went on to say, beyond observations of such reactions to multichannel sound technology, "we don't know very much". Research in psychoacoustics being used to develop virtual audio systems relies on such reactions and on a notion of human hardwiring for stimulus response (see Jones, "Sense"). But a major stumbling block toward the development of those systems is that none are able to account for individual listeners' perceptions. It is therefore important to consider the individual along with the social dimension in discussions of sound and music. For instance, the term "sound" is deployed in popular music to signify several things, all of which have to do with music or musical performance, but none of which is music. So, for instance, musical groups or performers can have a "sound", but it is distinguishable from what notes they play. Entire music scenes can have "sounds", but the music within such scenes is clearly distinct and differentiated. For the study of popular music this is a significant but often overlooked dimension. As Grossberg argues, "the authenticity of rock was measured by its sound" (207). Visually, he says, popular music is suspect and often inauthentic (sometimes purposefully so), and it is grounded in the aural. Similarly in country music Jensen notes that the "Nashville Sound" continually evoked conflicting definitions among fans and musicians, but that: The music itself was the arena in and through which claims about the Nashville Sound's authenticity were played out. A certain sound (steel guitar, with fiddle) was deemed "hard" or "pure" country, in spite of its own commercial history. (84) One should, therefore, attend to the interpretive acts associated with sound and its meaning. But why has not popular music studies engaged in systematic analysis of sound at the level of the individual as well as the social? As John Shepherd put it, "little cultural theoretical work in music is concerned with music's sounds" ("Value" 174). Why should this be a cause for concern? First, because Shepherd claims that sound is not "meaningful" in the traditional sense. Second, because it leads us to re-examine the question long set to the side in popular music studies: What is music? The structural homology, the connection between meaning and social formation, is a foundation upon which the concept of authenticity in popular music stands. Yet the ability to label a particular piece of music "good" shifts from moment to moment, and place to place. Frith understates the problem when he writes that "it is difficult ... to say how musical texts mean or represent something, and it is difficult to isolate structures of musical creation or control" (56). Shepherd attempts to overcome this difficulty by emphasising that: Music is a social medium in sound. What [this] means ... is that the sounds of music provide constantly moving and complex matrices of sounds in which individuals may invest their own meanings ... [however] while the matrices of sounds which seemingly constitute an individual "piece" of music can accommodate a range of meanings, and thereby allow for negotiability of meaning, they cannot accommodate all possible meanings. (Shepherd, "Art") It must be acknowledged that authenticity is constructed, and that in itself is an argument against the most common way to think of authenticity. If authenticity implies something about the "pure" state of an object or symbol then surely such a state is connected to some "objective" rendering, one not possible according to Shepherd's claims. In some sense, then, authenticity is autonomous, its materialisation springs not from any necessary connection to sound, image, text, but from individual acts of interpretation, typically within what in literary criticism has come to be known as "interpretive communities". It is not hard to illustrate the point by generalising and observing that rock's notion of authenticity is captured in terms of songwriting, but that songwriters are typically identified with places (e.g. Tin Pan Alley, the Brill Building, Liverpool, etc.). In this way there is an obvious connection between authenticity and authorship (see Jones, "Popular Music Studies") and geography (as well in terms of musical "scenes", e.g. the "Philly Sound", the "Sun Sound", etc.). The important thing to note is the resultant connection between the symbolic and the physical worlds rooted (pun intended) in geography. As Redhead & Street put it: The idea of "roots" refers to a number of aspects of the musical process. There is the audience in which the musician's career is rooted ... . Another notion of roots refers to music. Here the idea is that the sounds and the style of the music should continue to resemble the source from which it sprang ... . The issue ... can be detected in the argument of those who raise doubts about the use of musical high-technology by African artists. A final version of roots applies to the artist's sociological origins. (180) It is important, consequently, to note that new technologies, particularly ones associated with the distribution of music, are of increasing importance in regulating the tension between alienation and progress mentioned earlier, as they are technologies not simply of musical production and consumption, but of geography. That the tension they mediate is most readily apparent in legal skirmishes during an unsettled era for copyright law (see Brown) should not distract scholars from understanding their cultural significance. These technologies are, on the one hand, "liberating" (see Hayward, Young, and Marsh) insofar as they permit greater geographical "reach" and thus greater marketing opportunities (see Fromartz), but on the other hand they permit less commercial control, insofar as they permit digitised music to freely circulate without restriction or compensation, to the chagrin of copyright enthusiasts. They also create opportunities for musical collaboration (see Hayward) between performers in different zones of time and space, on a scale unmatched since the development of multitracking enabled the layering of sound. Most importantly, these technologies open spaces for the construction of authenticity that have hitherto been unavailable, particularly across distances that have largely separated cultures and fan communities (see Paul). The technologies of Internetworking provide yet another way to make connections between authenticity, music and sound. Community and locality (as Redhead & Street, as well as others like Sara Cohen and Ruth Finnegan, note) are the elements used by audience and artist alike to understand the authenticity of a performer or performance. The lived experience of an artist, in a particular nexus of time and space, is to be somehow communicated via music and interpreted "properly" by an audience. But technologies of Internetworking permit the construction of alternative spaces, times and identities. In no small way that has also been the situation with the mediation of music via most recordings. They are constructed with a sense of space, consumed within particular spaces, at particular times, in individual, most often private, settings. What the network technologies have wrought is a networked audience for music that is linked globally but rooted in the local. To put it another way, the range of possibilities when it comes to interpretive communities has widened, but the experience of music has not significantly shifted, that is, the listener experiences music individually, and locally. Musical activity, whether it is defined as cultural or commercial practice, is neither flat nor autonomous. It is marked by ever-changing tastes (hence not flat) but within an interpretive structure (via "interpretive communities"). Musical activity must be understood within the nexus of the complex relations between technical, commercial and cultural processes. As Jensen put it in her analysis of Patsy Cline's career: Those who write about culture production can treat it as a mechanical process, a strategic construction of material within technical or institutional systems, logical, rational, and calculated. But Patsy Cline's recording career shows, among other things, how this commodity production view must be linked to an understanding of culture as meaning something -- as defining, connecting, expressing, mattering to those who participate with it. (101) To achieve that type of understanding will require that popular music scholars understand authenticity and music in a symbolic realm. Rather than conceiving of authenticity as a limited resource (that is, there is only so much that is "pure" that can go around), it is important to foreground its symbolic and ever-changing character. Put another way, authenticity is not used by musician or audience simply to label something as such, but rather to mean something about music that matters at that moment. Authenticity therefore does not somehow "slip away", nor does a "pure" authentic exist. Authenticity in this regard is, as Baudrillard explains concerning mechanical reproduction, "conceived according to (its) very reproducibility ... there are models from which all forms proceed according to modulated differences" (56). Popular music scholars must carefully assess the affective dimensions of fans, musicians, and also record company executives, recording producers, and so on, to be sensitive to the deeply rooted construction of authenticity and authentic experience throughout musical processes. Only then will there emerge an understanding of the structures of feeling that are central to the experience of music. Footnotes For analyses of the Walkman's role in social settings and popular music consumption see du Gay; Hosokawa; and Chen. It has been thus since the advent of disc recording, when engineers would watch a record's grooves through a microscope lens as it was being cut to ensure grooves would not cross over one into another. References Altman, Rick. "Television/Sound." Studies in Entertainment. Ed. Tania Modleski. Bloomington: Indiana UP, 1986. 39-54. Baudrillard, Jean. Symbolic Death and Exchange. London: Sage, 1993. Brown, Ronald. Intellectual Property and the National Information Infrastructure: The Report of the Working Group on Intellectual Property Rights. Washington, DC: U.S. Department of Commerce, 1995. Chen, Shing-Ling. "Electronic Narcissism: College Students' Experiences of Walkman Listening." Annual meeting of the International Communication Association. Washington, D.C. 1993. Du Gay, Paul, et al. Doing Cultural Studies. London: Sage, 1997. Frith, Simon. Sound Effects. New York: Pantheon, 1981. Fromartz, Steven. "Starts-ups Sell Garage Bands, Bowie on Web." Reuters newswire, 4 Dec. 1996. Grossberg, Lawrence. We Gotta Get Out of This Place. London: Routledge, 1992. Hayward, Philip. "Enterprise on the New Frontier." Convergence 1.2 (Winter 1995): 29-44. Hosokawa, Shuhei. "The Walkman Effect." Popular Music 4 (1984). Jensen, Joli. The Nashville Sound: Authenticity, Commercialisation and Country Music. Nashville, Vanderbilt UP, 1998. Jones, Steve. Rock Formation: Music, Technology and Mass Communication. Newbury Park, CA: Sage, 1992. ---. "Popular Music Studies and Critical Legal Studies" Stanford Humanities Review 3.2 (Fall 1993): 77-90. ---. "A Sense of Space: Virtual Reality, Authenticity and the Aural." Critical Studies in Mass Communication 10.3 (Sep. 1993), 238-52. ---. "Sound, Space & Digitisation." Media Information Australia 67 (Feb. 1993): 83-91. Marrsh, Brian. "Musicians Adopt Technology to Market Their Skills." Wall Street Journal 14 Oct. 1994: C2. Massenburg, George. "Recording the Future." EQ (Apr. 1997): 79-80. Paul, Frank. "R&B: Soul Music Fans Make Cyberspace Their Meeting Place." Reuters newswire, 11 July 1996. Redhead, Steve, and John Street. "Have I the Right? Legitimacy, Authenticity and Community in Folk's Politics." Popular Music 8.2 (1989). Shepherd, John. "Art, Culture and Interdisciplinarity." Davidson Dunston Research Lecture. Carleton University, Canada. 3 May 1992. ---. "Value and Power in Music." The Sound of Music: Meaning and Power in Culture. Eds. John Shepherd and Peter Wicke. Cambridge: Polity, 1993. Silverman, Kaja. The Acoustic Mirror. Bloomington: Indiana UP, 1988. Sobchack, Vivian. Screening Space. New York: Ungar, 1982. Young, Charles. "Aussie Artists Use Internet and Bootleg CDs to Protect Rights." Pro Sound News July 1995. Citation reference for this article MLA style: Steve Jones. "Seeing Sound, Hearing Image: 'Remixing' Authenticity in Popular Music Studies." M/C: A Journal of Media and Culture 2.4 (1999). [your date of access] <http://www.uq.edu.au/mc/9906/remix.php>. Chicago style: Steve Jones, "Seeing Sound, Hearing Image: 'Remixing' Authenticity in Popular Music Studies," M/C: A Journal of Media and Culture 2, no. 4 (1999), <http://www.uq.edu.au/mc/9906/remix.php> ([your date of access]). APA style: Steve Jones. (1999) Seeing Sound, Hearing Image: "Remixing" Authenticity in Popular Music Studies. M/C: A Journal of Media and Culture 2(4). <http://www.uq.edu.au/mc/9906/remix.php> ([your date of access]).

In the 16th century Philippines, the marriage of the Church and the State was the dominant set-up by virtue of Spain’s quest for colonization and evangelization. Civil administrators and church missionaries were called to cooperate the will of the king. Inmost cases, their point of contact was also the area of friction because of their opposing intentions. The early Spanish missionaries in the 16th century Philippines were influenced by the teachings of Bartolome de Las Casas and Vitoria that ignited them to confront their civil counterparts who were after getting the wealth and resources of the natives at the expense of their dignity and rights. Since the King showed interest in protecting the rights of the Indians, Churchmen used legal procedures, reports and personaltestimonies in the Royal Court to create changes in the systems employed in the islands. The relationship between the Spaniards and the natives cannot be reduced to a monolithic relationship between the two races. The power dynamics should be viewed within the plethora of groups who were engaged in the discourse including the bishop of Manila, governor-general, encomenderos, adelantados, soldiers, religious orders, native leaders and even the common indios. Given the canvas of conflicting motives, the proponents of conquests and missionary undertakings grappled to persuade the Spanish Royal Court to take their respective stand on the disputed human rights and justice issues on the legitimacy of the conquest, tributes, slavery and forced labor. References Primary Documentary Sources Anales Ecclesiasticos de Philipinas: 1574-1682. Volume 1. Manila: Archdioceseof Manila Archives, 1994. Arancel. Quezon City: Archivo de la Provincia del Santo Rosario (APSR), MSTomo 3, Doc.3. Blair, Emma Helen and Robertson Alexander, eds. at annots. The Philippine Islands,1493-1898: Explorations by Early Navigators, Descriptions ofthe Islands and Their Peoples, their History and Records of the CatholicMissions, as related in Contemporaneous Books and ManuscriptsShowing the Political, Economic, Commercial and Religious Conditionsof Those Islands from Their Earliest Conditions with European Nationsto the Close of the Nineteenth Century. 55 Volumes. Cleveland: ArthurH Clark, 1903-1909. Hereinafter referred to as B and R. The followingprimary documents were used in this dissertation: Colin-Pastells. LaborEvangelica I. Historical Conservation Society. The Christianizationof the Philippines. Manila: Historical Conservation Society, 1965. Keen, Benjamin, Editor. Latin American Civilization: History and Society, 1492to the Present. London: Westview Press, 1986. Las Casas, Bartolome. Historia de las Indias. Mexico, 1951. __________________. The Spanish Colonie. University Microfilms Inc., 1996.Licuanan, Virginia Benitez and Mira Jose Llavador, eds and annots. PhilippinesUnder Spain. 6 Volumes. Manila: National Trust for Historical and Cultural Preservation of the Philippines, 1996. Munoz Text of Alcina’s History of the Bisayan Islands (1668). Translated byPaul S. Lietz. Chicago: Philippine Studies Program, 1960. National Historical Commission, Coleccion de Documentos Ineditos de Ultramar,Madrid, 1887. Navarette, Martin Fernandez D. Colleccion de los Viajes y descubrimientos queHicieron por mar los espanoles desde fines del siglo XV. Madrid: 1825-1837. Pastells, Pablo. Historia General de Filipinas in Catalogo de los DocumentosRelativos a las Islas Filipinas. Barcelona, 1925. Recopilacion de Leyes de los Reynos de las Indias. Tomo I. Madrid, 1943.San Agustin, Gaspar de. Conquistas de las Islas Filipinas: 1565-1615. Translatedby Luis Antonio Maneru. Bilingual Edition. Manila: San Agustin Museum, 1998. Zaide, Gregorio, eds. at annots. Documentary Sources of Philippine History. 14Volumes. Manila: National Bookstore, 1990. Secondary Sources Books Chan, Manuel T. The Audiencia and the Legal System in the Philippines (1583-1900). Manila: Progressive Printing Palace, Inc., 1998. Cunningham, Charles Henry. The Audiencia in the Spanish Colonies: AsIllustrated by the Audiencia of Manila 1583-1800. Berkeley: Universityof California Press, 1919. Cushner, Nicolas P. The Isles of the West: Early Spanish Voyages to thePhilippines, 1521-1564. Quezon City: Ateneo de Manila Press, 1966. _________________. Spain in the Philippines: From Conquest to the Revolution. Aberdeen:Cathay Press Ltd., 1971. De la Costa, Horacio. Jesuits in the Philippines. Cambridge: Harvard UniversityPress, 1961. De la Rosa, Rolando V. Beginnings of the Filipino Dominicans. Manila: USTPress, 1990. Fernandez, Pablo. History of the Church in the Philippines. Manila: NationalBookstore, 1979. Gutierrez, Lucio, O.P. Domingo Salazar, OP First Bishop of the Philippines: 1512-1594. Manila: University of Santo Tomas Press, 2001. Haring, C.H. The Spanish Empire in America. New York: Harcourt, Brace andWorld Inc., 1963. Keen, Banjamin. A History of Latin America, 5th Edition. Vol.1. Boston: HoughtonMifflin Company, 1996. Keller, Albert Galloway. Colonization. Boston: 1908. Luengo, Josemaria. A History of Manila-Acapulco Slave Trade (1565-1815). Bohol:Mater Dei Publications, 1996. Munoz, Honorio. Vitoria and the Conquest of America: A Study on the FirstReading on the Indians. Manila: UST Press, 1938. _____________. Vitoria and War: A Study on the Second Reading on the Indians oron the Right of War. Manila: UST Press, 1937. Noone, Martin. The Islands Saw It.1521-1581. Ireland: Helicon Press, 1982. Pitrie, Sir Charles. Philip II of Spain. London: Eyre and Spottiswoode, 1963. Porras, Jose Luis. The Synod of Manila of 1582. Translated by Barranco, Carballo,Echevarra, Felix, Powell and Syquia. Manila: Historical Conservation Society, 1990. Rafael. Vicente. Contracting Colonialism. Quezon City: Ateneo de Manila Press, 1998. Santiago, Luciano P.R. To Love and To Suffer: The Development of theReligious Congregations for Women in the Spanish Philippines, 1565-1898. Quezon City: Ateneo de Manila Press, 2005. Scott, J.B. Francisco de Vitoria and His Law of Nations. Oxford, 1934.Scott, William Henry. Slavery in the Spanish Philippines. Manila: De la Salle UniversityPress, 1991. Shumway, David. Michel Foucault. Virginia: G. K. Hall and Co., 1989. Simpson, Lesley Byrd. The Encomienda in New Spain: The Beginning ofSpanish Mexico. Berkeley: University of California Press, 1966. Sitoy, Valentino Jr. The Initial Encounter: a History of Christianity in the Philippines,Vol. 1. Quezon City: New Day Publishers, 1985. Zafra, Nicolas. Readings in Philippine History. Manila. University of the Philippines, 1947. Zaide, Gregorio F. The Pageant of Philippine History Vol. 1. Manila: 1979. Articles Arcilla, Jose S. S.J., The Spanish Conquest. Kasaysayan: The Story of theFilipino People Vol. 3. Hongkong: C & C Offset Printing Co., Ltd, 1998. Bernal, Rafael. “Introduction.” The Colonization and Conquest of the Philippinesby Spain: Some Contemporary Source Documents. Manila: FilipinianaBook Guild, 1965. Burkholder, Mark A. “Sepulveda, Juan Gines de.” Encyclopedia of Latin AmericanHistory and Culture Vol.5. Edited by Barbara A. Tenenbaum. NewYork: Macmillan Library Reference, 1996. Burkholder, Susanne Hiles. “Vitoria, Francisco de.” Encyclopedia of Latin AmericanHistory and Culture Vol.5 Edited by Barbara A. Tenenbaum.New York: Macmillan Library Reference, 1996. De Jesus, Edilberto. “Christianity and Conquest: The Basis of Spanish SovereigntyOver the Philippines.” The Beginnings of Christianity in the Philippines.Manila: Philippine Historical Institute, 1965. Donovan, William. “Las Casas, Bartolome.” Encyclopedia of Latin American Historyand Culture Vol.3. Edited by Barbara A. Tenenbaum. New York:Macmillan Library Reference, 1996. Gutierrez, Lucio. “Domingo de Salazar’s Struggle for Justice and Humanizationin the Conquest of the Philippines.” Philippiniana Sacra 14, 1975. ____________. “Domingo de Salazar, OP, First Bishop of the Philippines (1512-1594): Defender of the Rights of the Filipinos at the Spanish Contact”Philippiniana Sacra XX, 1979. ____________. “Domingo de Salazar’s Memorial of 1582 on the Status of the Philippines:A Manifesto for Freedom and Humanization.” Philippiniana SacraVol. 21, No. 63, 1986. ___________. “Opinion of Fr. Domingo de Salazar, O.P. First Bishop of the Philippinesand the Major Religious Superiors Regarding Slaves.” PhilippinianaSacra Vol. 22, No. 64, 1986. ___________. “The Synod of Manila: 1581-1586.” Philippiniana Sacra Vol. XXV, No.74, 1990. Keith, Robert G. “Encomienda,Hacienda and Corregimiento in Spanish America:A Structural Analysis.” Hispanic American Historical Review 51:pp.110-116. Kirkpatrick, F. A. “Repartimiento-Encomienda.” Hispanic American HistoricalReview XIX: pp.373-379. Pastrana, Apolinar. “The Franciscans and the Evangelization of the Philippines(1578-1900).” Boletin Eclesiastico de Filipinas, 29, Jan-Feb 1965:pp.83-85. Quirk, Robert E. “Some Notes on a Controversial Controversy: Juan Gines deSepulveda and Natural Servitude.” Hispanic American Historical ReviewVol.XXXIV No.3 August 1954: 358. Ramirez, Susan S. “Encomienda.” Encyclopedia of Latin American History andCulture, Vol.2 Edited by Barbara A. Tenenbaum. New York: MacmillanLibrary Reference, 1996. Schwaller, John F. “Patronato Real”. Encyclopedia in Latin American History andCulture, Vol.4. Edited by Barbara a. Tenenbaum. New York: MacmillanLibrary Reference, 1996. Scott. William Henry. “Why did Tupas betray Dagami?” Philippine Quarterly ofCulture and Society 14 (1986): p.24. Villaroel, Fidel. “The Church and the Philippine Referendum of 1599.” PhilippinianaSacra Vol.XXXV 2000: pp.89-128. Internet Source Hyperdictionary. http://www. hyperdictionary.com/dictionary/politics, accessedon 18 December 2004. Human Rights Watch World Report for Philippines, 2017 https://www.hrw.org/world-report/2017/country-chapters/philippines. General References Encyclopedia of Latin American History and Culture, Volume 1-5. Edited byBarbara A. Tenebaum. New York: Macmillan Library Reference, 1996. Kasaysayan: The Story of the Filipino People ,Vol. 3 The Spanish Conquest.Hongkong: Asia Publishing Company Limited, 1998. Unpublished Materials Cabezon, Antonio. An Introduction to Church and State Relations According toFrancisco Vitoria. Unpublished Thesis: University of Sto. Tomas, 1964. De la Costa, Horacio. Jurisdictional Conflicts in the Philippines During the XVIand the XVII Centuries. Harvard: Unpublished Dissertation, 1951.

Resources currently available to the scientific community that may be of interest for endocrinology research are described briefly here. More information is available through The Endocrine Society Home Page (http://www.endo-society.org) or the information provided below. HUMAN TISSUE AND BIOLOGIC SPECIMEN RESOURCES NCI - Cooperative Human Tissue Network (CHTN) The NCI Cooperative Human Tissue Network (CHTN) provides normal, benign, precancerous, and cancerous human tissue to the scientific community for biomedical research. Specimens are collected according to the investigator’s individual protocol. Information provided with the specimens includes routine histopathologic and demographic data. The CHTN can also provide a variety of tissue microarrays. Contact the CHTN Web site at http://www-chtn.ims.nci.nih.gov, or 1-866-GO2-CHTN (1-866-462-2486). NCI - Cooperative Breast Cancer Tissue Resource (CBCTR) The NCI Cooperative Breast Cancer Tissue Resource (CBCTR) can provide researchers with access to formalin-fixed, paraffin-embedded primary breast cancer specimens, with associated pathologic, clinical, and outcome data. All specimens are evaluated for pathologic diagnosis by CBCTR pathologists using standard diagnostic criteria. The collection is particularly well suited for validation studies of diagnostic and prognostic markers. The CBCTR also makes available breast cancer tissue microarrays designed by NCI statisticians to provide high statistical power for studies of stage-specific markers of breast cancer. Contact CBCTR’s Web site at http://cbctr.nci.nih.gov, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com NCI - Cooperative Prostate Cancer Tissue Resource (CPCTR) The NCI Cooperative Prostate Cancer Tissue Resource (CPCTR) can provide access to over 4,000 cases of formalin-fixed, paraffin-embedded primary prostate cancer specimens, with associated pathology and clinical data. Fresh-frozen tissue is also available with limited clinical follow-up information. In addition, slides from prostate cancer tissue microarrays with associated pathology and clinical data are now available. Contact the CPCTR Web site at http://www.prostatetissues.org, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com NCI - AIDS and Cancer Specimen Resource (ACSR) The AIDS and Cancer Specimen Resource (ACSR) provides qualified researchers with tissue, cell, blood, and fluid specimens, as well as clinical data from patients with AIDS and cancer. The specimens and clinical data are available for research studies, particularly those that translate basic research findings to clinical application. Contact the ACSR Web site (http://acsr.ucsf.edu/) or Dr. Kishor Bhatia, (301) 496-7147; e-mail: bhatiak@mail.nih.gov NCI - Breast and Ovarian Cancer Family Registries (CFRs) The Breast and Ovarian CFRs facilitate and support interdisciplinary and population-based research on the identification and characterization of breast and ovarian cancer susceptibility genes, with particular emphasis on gene-gene and gene-environment interaction research. Available from the registries are: a) family history, epidemiologic and clinical data, b) updates on cancer recurrence, morbidity and mortality in participating families, and c) biospecimens, including plasma, lymphocytes, serum, DNA, Guthrie cards or buccal smears, and paraffin blocks of tumor tissue. For further information on these registries, contact the CFR Web site (http://epi.grants.cancer.gov/BCFR) or (301) 496-9600. NCI - Specimen Resource Locator The NCI Specimen Resource Locator (http://cancer.gov/specimens) is a database that helps researchers locate specimens for research. The database includes resources such as tissue banks and tissue procurement systems with access to normal, benign, precancerous, and/or cancerous human tissue covering a wide variety of organ sites. Researchers specify the types of specimens, number of cases, preservation methods, and associated data they require. The Locator will search the database and return a list of tissue resources most likely to meet their requirements. When no match is obtained, the researcher is referred to the NCI Tissue Expediter [(301) 496-7147; e-mail: tissexp@mail.nih.gov]. The Tissue Expediter is a scientist who can help match researchers with appropriate resources or identify appropriate collaborators when those are necessary. NIDDK - Biologic Samples from Diabetic Study Foundation A portion (1/3) of all stored nonrenewable samples (plasma, serum, urine) from subjects enrolled in the Diabetes Control and Complications Trial (DCCT) is available for use by the scientific community to address questions for which these samples may be invaluable. Announcements for using this resource appear in the NIH Guide for Grants and Contracts periodically. Inquiries may be addressed to: Catherine C. Cowie, Ph.D., Director, Diabetes Epidemiology Program, NIDDK, 6707 Democracy Blvd., Room 691, MSC 5460, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892-5460. Phone: (301) 594-8804; fax: (301) 480-3503; e-mail: cowiec@extra.niddk.nih.gov NIDDK - NIDDK Central Repositories (Diabetes Prevention Study) The NIDDK Central Repositories have selected biosamples from the DPT-1 (The Diabetes Prevention Type 1) study that are available to qualified investigators through an application process. These samples are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. Information about how to apply for these materials can be obtained from the NIDDK Central Repositories by contacting Ms. Helen Ray of RTI, 1-919-316-3418, or hmp@rti.org. Direct scientific-technical inquiry to the Project Officer of the NIDDK Central Repositories, Dr. Rebekah Rasooly, at phone: (301) 594-6007; e-mail: rr185i@nih.gov Visit the Repositories Web site at http://www.niddkrepository.org. NICHD - Brain and Tissue Bank for Developmental Disorders The purpose of the Bank is to collect, preserve, and distribute human tissues to investigators interested in autism and developmental disorders; normal tissues may be available for other research purposes. Further information can be obtained at www.btbank.org. The contact persons are H. Ron Zielke or Sally Wisniewsky, University of Maryland (1-800-847-1539), and Carol Petito or Stephanie Lojko, University of Miami (1-800-592-7246). NICHD - Reproductive Tissue Sample Repository (RTSaR) The Reproductive Tissue Sample Repository (RTSaR) is a virtual repository with online tissue sample acquisition capabilities. The RTSaR provides investigators with real-time access to human and nonhuman primate tissue and fluid inventories from four tissue bank facilities that are supported through the Specialized Cooperative Centers Program in Reproduction Research. The tissue banks are located at the University of California, San Diego (human ovary bank), Stanford University (human endometrium and DNA bank), Johns Hopkins University (male reproductive tissues and fluids), and the Oregon National Primate Research Center (nonhuman primate tissues). The web site for the RTSaR is https://rtsar.nichd.nih.gov/rtsar/login. If you wish to access the RTSaR, you can request an id and password to access the system by contacting the network administrator at RTSaR@mail.nih.gov Once you access the system, contact information for each bank is provided. Access is open to all investigators living in North America who are supported by research and research training grants from the NIH. One id and password will be provided to each principal investigator that can be utilized by any person working in the P.I.’s laboratory, or, in the case of institutional training grants (T32) and institutional career development award programs (K12), any person supported by the aforementioned awards. NCRR - Human Tissues and Organs Resource (HTOR) The Human Tissues and Organs Resource (HTOR) cooperative agreement supports a procurement network developed by the National Disease Research Interchange (NDRI), a not-for-profit organization. By collaborating with various medical centers, hospitals, pathology services, eye banks, tissue banks, and organ procurement organizations, HTOR provides a wide variety of human tissues and organs—both diseased and normal—to researchers for laboratory studies. Such samples include tissues from the central nervous system and brain, cardiovascular system, endocrine system, eyes, bone, and cartilage. For further information, consult the NDRI Web site (www.ndri.com) or contact Dr. John T. Lonsdale at NDRI, 8 Penn Center, 8th Floor, 1628 JFK Boulevard, Philadelphia, PA 19103. Phone: (800) 222-6374, ext. 271; fax: (215) 557-7154; e-mail: jlonsdale@ndriresource.org The NDRI Web site is http://www.ndri.com. NCRR - Islet Cell Resource (ICR) With support from NCRR, 10 Islet Cell Resource (ICR) centers isolate, purify, and characterize human pancreatic islets for subsequent transplantation into patients with type I diabetes. The ICR centers procure whole pancreata and acquire relevant data about donors; improve islet isolation and purification techniques; distribute islets for use in approved clinical protocols; and perfect the methods of storage and shipping. In this way, the centers optimize the viability, function, and availability of islets and help clinical researchers capitalize on the recently reported successes in islet transplantation. Information on submitting requests for islet cells can be obtained from Mr. John Kaddis, ICR Coordinating Center Project Manager, City of Hope National Medical Center, 1500 E. Duarte Road, Duarte, California 91010. Phone (626) 359-8111, ext. 63377; fax: (626) 471-7106; e-mail: jkaddis@coh.org The Coordinating Center hosts a Web site at http://icr.coh.org. NIA - SWAN Repository (longitudinal, multiethnic study of women at midlife including the menopausal transition) The SWAN Repository is a biologic specimen bank of the Study of Women’s Health Across the Nation (SWAN). The SWAN cohort was recruited in 1996/1997 and consists of 3302 African-American, Caucasian, Chinese, Hispanic, and Japanese women. The SWAN Repository contains more than 350,000 blood and urine specimens generated from the study participants’ annual visits (8 visits to date), at which time medical and health history, psychosocial measures, biological measures, and anthropometric data were and are being collected. In addition, a subset of the participants are providing urine samples, collected daily over the length of one menstrual cycle, each year. More than 900,000 of these samples are in the SWAN Repository and are available to researchers who wish to study the midlife and menopausal transition. Additionally, a DNA sample repository is also available and includes DNA as well as transformed B-lymphoblastoid cell lines from more than 1800 of the participants. To learn more about the SWAN Repository and how to apply to use SWAN Repository specimens, contact the Web site at http://www. swanrepository.com or Dr. MaryFran Sowers, University of Michigan, School of Public Health, Epidemiology Dept., (734) 936-3892; e-mail: mfsowers@umich.edu HUMAN AND ANIMAL CELL AND BIOLOGIC REAGENT RESOURCES NIDDK - National Hormone and Peptide Program The National Hormone and Peptide Program (NHPP) offers peptide hormones and their antisera, tissues (rat hypothalami), and miscellaneous reagents to qualified investigators. These reagents are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. These materials can be obtained from Dr. A. F. Parlow of the Harbor-UCLA Medical Center, Research and Education Institute, Torrance, CA. A more complete description of resources within this program is provided in The Endocrine Society journals. Direct scientific-technical inquiry to NHPP Scientific Director, Dr. Al Parlow, at phone: (310) 222-3537; fax: (310) 222-3432; e-mail: parlow@humc.edu Visit the NHPP Web site at http://www.humc.edu/hormones. NICHD - National Hormone and Pituitary Program (see NIDDK listing) Following is a list of reagents currently available through the resources of NICHD: Androgen receptor and peptide antigen Recombinant monkey (cynomolgus) and baboon luteinizing hormone and follicle-stimulating hormone and antisera. NIA - Aging Cell Bank To facilitate aging research on cells in culture, the NIA provides support for the Aging Cell Bank located at the Coriell Institute for Medical Research in Camden, NJ. The Aged Cell Bank provides fibroblast, lymphoblastoid, and differentiated cell lines from a wide range of human age-related conditions and other mammalian species, as well as DNA from a limited subset of cell lines. For further information, the Aged Cell Bank catalog can be accessed at http://locus.umdnj.edu/nia or contact Dr. Donald Coppock at 1-800-752-3805. NCRR - Various Cell Repositories NCRR maintains the following cell repository resources: National Cell Culture Center, National Stem Cell Resource, and the Yeast Genetic Stock Center. Further information regarding these resources may be obtained through the NCRR Web site at: www.ncrr.nih.gov/ncrrprog/cmpdir/BIOLOG.asp. ANIMAL RESOURCES NIA - Aging Rodent Resources NIA maintains both rat and mouse colonies for use by the scientific community. The animals available range in age from 1 to 36 months. A repository of fresh-frozen tissue from the NIA aged rodent colonies is stocked with tissue from mouse and rat strains, including caloric-restricted BALB/c mice. The NIA also maintains a colony of calorically restricted rodents of selected genotypes, which are available to the scientific community. For further information, please refer to the Aged Rodent information handbook at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentColoniesHandbook/ or contact the Office of Biological Resources and Resource Development order desk. Phone: (301) 496-0181; fax: (301) 402-5597; e-mail: rodents@nia.nih.gov NIA - Aged Rodent Tissue Bank The rodent tissue bank contains flash-frozen tissues from rodents in the NIA aged rodent colonies. Tissue is collected from rodents at 4 or 5 age points throughout the lifespan. Tissue arrays are also available. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentTissueBankHandbook/. NCRR - Mutant Mouse Regional Resource Centers (MMRRC) The Mutant Mouse Regional Resource Center (MMRRC) Program consists of centers that collectively operate as a one-stop shop to serve the biomedical research community. Investigators who have created select mutant mouse models may donate their models to an MMRRC for broad dissemination to other investigators who request them for noncommercial research investigations related to human health, disease, and treatments. The NCRR Division of Comparative Medicine (DCM) supports the MMRRCs, which are electronically linked through the MMRRC Informatics Coordinating Center (ICC) to function as one facility. The ICC, located at The Jackson Laboratory in Bar Harbor, ME, provides database and other informatics support to the MMRRC to give the research community a single entry point to the program. Further information can be obtained from the Web site at http://www.mmrrc.org, or from Franziska Grieder, D.V.M., Ph.D., Division of Comparative Medicine, NCRR. Phone (301) 435-0744; fax: (301) 480-3819; e-mail: griederf@ncrr.nih.gov NCRR - Induced Mutant Mouse Resource (IMR) The Induced Mutant Mouse Resource (IMR) at The Jackson Laboratory provides researchers with genetically engineered mice (transgenic, targeted mutant, retroviral insertional mutant, and chemically induced mutant mice). The function of the IMR is to select, import, cryopreserve, maintain, and distribute these important strains of mice to the research community. To improve their value for research, the IMR also undertakes genetic development of stocks, such as transferring mutant genes or transgenes to defined genetic backgrounds and combining transgenes and/or targeted mutations to create new mouse models for research. Over 800 mutant stocks have been accepted by the IMR. Current holdings include models for research on cancer, immunological and inflammatory diseases, neurological diseases and behavioral disorders, cardiovascular diseases, developmental disorders, metabolic and other diseases, reporter (e.g. GFP) and recombinase (e.g. cre/loxP) strains. About 8 strains a month are being added to the IMR holdings. A list of all strains may be obtained from the IMR Web site: www.jax.org/resources/documents/imr/. Online submission forms are also available on that site. All mice can be ordered by calling The Jackson Laboratory’s Customer Service Department at 1-800-422-MICE or (207) 288-5845 or by faxing (207) 288-6150. NIDDK - Mouse Metabolic Phenotyping Centers The mission of the Mouse Metabolic Phenotyping Centers is to provide the scientific community with standardized, high-quality metabolic and physiologic phenotyping services for mouse models of diabetes, diabetic complications, obesity, and related disorders. Researchers can ship mice to one of the four Centers (University of Cincinnati, University of Texas Southwestern Medical Center, Vanderbilt University, and Yale University) and obtain on a fee-for-service basis a range of complex exams used to characterize mouse metabolism, blood composition, energy balance, eating and exercise, organ function and morphology, physiology, and histology. Many tests are done in living animals and are designed to elucidate the subtle hallmarks of metabolic disease. Information, including a complete list of available tests, can be found at www.mmpc.org, or contact Dr. Maren R. Laughlin, NIDDK, at (301) 594-8802; e-mail: Maren.Laughlin@nih.gov; or Dr. Kristin Abraham, NIDDK, at (301) 451-8048; e-mail: abrahamk@extra.niddk.nih.gov NCRR - National Primate Research Centers (NPRCs) National Primate Research Centers (NPRCs) are a network of eight highly specialized facilities for nonhuman primates (NHP) research. Funded by grants through NCRR’s Division of Comparative Medicine (DCM), each center, staffed with experienced research and support staff, provides the appropriate research environment to foster the development of NHP models of human health and disease for biomedical investigations. The NPRCs are affiliated with academic institutions and are accessible to eligible biomedical and behavioral investigators supported by research project grants from the National Institutes of Health and other sources. Further information may be obtained from the notice, Procedures for Accessing Regional Primate Research Centers, published in the NIH Guide for Grants and Contracts at http://grants2.nih.gov/grants/guide/notice-files/not97-014.html, or from John Harding, Ph.D., National Primate Research Centers and AIDS Animal Models Program, Division of Comparative Medicine, NCRR. Phone: (301) 435-0744; fax: (301) 480-3819; e-mail: hardingj@mail.nih.gov NIA - Nonhuman Primates, Aging Set-Aside Colony NIA maintains approximately 200 nonhuman primates (M. mulatta) at four National Primate Research Centers (see above) for conducting research on aging. These animals range in age from 18 to 35 years. While these animals are predominantly reserved for non-invasive research, exceptions can be made to this policy. For further information, please contact Dr. Nancy Nadon, Office of Biological Resources and Resource Development, NIA. Phone: (301) 402-7744; fax: (301) 402-0010; e-mail: nadonn@nia.nih.gov NIA - Nonhuman Primate (NHP) Tissue Bank and Aging Database The NIA developed two new resources to facilitate research in the NHP model. The NHP tissue bank contains fresh-frozen and fixed tissue donated by primate centers around the country. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/NHPTissueBankHandbook.htm. The Primate Aging Database provides an internet accessible database with data from thousands of primates around the country. It can be used to investigate the effect of age on a variety of parameters, predominantly blood chemistry and husbandry measurements. The site is password protected. The URL is http://ipad.primate.wisc.edu. NIA - Obesity, Diabetes and Aging Animal Resource (USF-ODARC) The NIA supports a colony of aged rhesus macaques, many of which are obese and/or diabetic. This is a long-term colony of monkeys housed at the University of South Florida’s Obesity, Diabetes and Aging Research Center. They have been extensively and longitudinally characterized for general health variables, blood chemistry, food intake, and body weight. Diabetic monkeys are tested daily for urine glucose and ketone levels, and prediabetic monkeys are tested weekly. Data for some of the monkeys extend as far back as 15 years. This unique resource is available for collaborative studies. ODARC has a significant amount of stored tissue collected at necropsy and stored blood/plasma collected longitudinally. Serial blood collection or tissue collection at necropsy can also be performed prospectively. Testing and imaging can also be performed on the monkeys. Inquiries regarding collaborative studies using the ODARC colony should be directed to: Barbara C. Hansen, Ph.D., Director, Obesity, Diabetes and Aging Research Center, University of South Florida, All Children’s Hospital, 801 6th Street South #9340, St. Petersburg, FL 33701. Phone: (727) 767-6993; fax: (727) 767-7443; e-mail: bchansen@aol.com NCRR - Various Animal Resources NCRR maintains the following animal resources: Animal Models and Genetic Stocks, Chimpanzee Biomedical Research Program, NIH Animal Genetic Resource, and the Specific Pathogen Free Macaque Breeding and Research Program. Further information regarding these and other resources may be obtained through the NCRR Web site at www.ncrr.nih.gov/comparative_med.asp. MISCELLANEOUS RESOURCES NCRR - National Gene Vector Laboratories (NGVLs) The National Gene Vector Laboratories (NGVLs), with core funding from NCRR, serve as a resource for researchers to obtain adequate quantities of clinical-grade vectors for human gene transfer protocols. The vector types include retrovirus, lentivirus, adenovirus, adeno-associated virus, herpes-virus, and DNA plasmids. The NGVLs consist of three vector production centers at: Baylor College of Medicine; City of Hope National Medical Center and Beckman Research Institute; and Indiana University, which also serves as the Coordinating Center for all the laboratories. Two additional laboratories conduct toxicology studies for NGVL-approved investigators. These laboratories are located at the Southern Research Institute and the University of Florida. Additional information about the process for requesting vector production and/or pharmacology/toxicology support should be directed to Ms. Lorraine Matheson, NGVL Project Coordinator, Indiana University School of Medicine. Phone: (317) 274-4519; fax: (317) 278-4518; e-mail: lrubin@iupui.edu The NGVL Coordinating Center at Indiana University also hosts a Web site at http://www.ngvl.org. NCRR - General Clinical Research Centers (GCRCs) The General Clinical Research Centers (GCRCs) are a national network of 82 centers that provide optimal settings for medical investigators to conduct safe, controlled, state-of-the-art in-patient and out-patient studies of both children and adults. GCRCs also provide infrastructure and resources that support several career development opportunities. Investigators who have research project funding from the National Institutes of Health (NIH) and other peer-reviewed sources may apply to use GCRCs. Because the GCRCs support a full spectrum of patient-oriented scientific inquiry, researchers who use these centers can benefit from collaborative, multidisciplinary research opportunities. To request access to a GCRC facility, eligible investigators should initially contact a GCRC program director, listed in the National Center for Research Resources (NCRR) Clinical Research Resources Directory (www.ncrr.nih.gov/ncrrprog/clindir/crdirectory.asp). Further information can be obtained from Anthony R. Hayward, M.D., Director, Division for Clinical Research Resources, National Center for Research Resources at NIH. Phone: (301) 435-0790; e-mail: haywarda@ncrr.nih.gov

Resources currently available to the scientific community that may be of interest for endocrinology research are described briefly here. More information is available through The Endocrine Society Home Page (http://www.endo-society.org) or the information provided below. Human Tissue and Biologic Specimen Resources NCI - Cooperative Human Tissue Network (CHTN) The NCI Cooperative Human Tissue Network (CHTN) provides normal, benign, precancerous, and cancerous human tissue to the scientific community for biomedical research. Specimens are collected according to the investigator’s individual protocol. Information provided with the specimens includes routine histopathologic and demographic data. The CHTN can also provide a variety of tissue microarrays. Contact the CHTN Web site at http://www-chtn.ims.nci.nih.gov, or 1-866-GO2-CHTN (1-866-462-2486). NCI - Cooperative Breast Cancer Tissue Resource (CBCTR) The NCI Cooperative Breast Cancer Tissue Resource (CBCTR) can provide researchers with access to formalin-fixed, paraffin-embedded primary breast cancer specimens, with associated pathologic, clinical, and outcome data. All specimens are evaluated for pathologic diagnosis by CBCTR pathologists using standard diagnostic criteria. The collection is particularly well suited for validation studies of diagnostic and prognostic markers. The CBCTR also makes available breast cancer tissue microarrays designed by NCI statisticians to provide high statistical power for studies of stage-specific markers of breast cancer. Contact CBCTR’s Web site at http://cbctr.nci.nih.gov, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com. NCI - Cooperative Prostate Cancer Tissue Resource (CPCTR) The NCI Cooperative Prostate Cancer Tissue Resource (CPCTR) can provide access to over 4,000 cases of formalin-fixed, paraffin-embedded primary prostate cancer specimens, with associated pathology and clinical data. Fresh-frozen tissue is also available with limited clinical follow-up information. In addition, slides from prostate cancer tissue microarrays with associated pathology and clinical data are now available. Contact the CPCTR Web site at http://www.prostatetissues.org, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com. NCI - AIDS and Cancer Specimen Resource (ACSR) The AIDS and Cancer Specimen Resource (ACSR) provides qualified researchers with tissue, cell, blood, and fluid specimens, as well as clinical data from patients with AIDS and cancer. The specimens and clinical data are available for research studies, particularly those that translate basic research findings to clinical application. Contact the ACSR Web site (http://acsr.ucsf.edu/) or Dr. Kishor Bhatia, (301) 496-7147; e-mail: bhatiak@mail.nih.gov. NCI - Breast and Ovarian Cancer Family Registries (CFRs) The Breast and Ovarian CFRs facilitate and support interdisciplinary and population-based research on the identification and characterization of breast and ovarian cancer susceptibility genes, with particular emphasis on gene-gene and gene-environment interaction research. Available from the registries are: a) family history, epidemiologic and clinical data, b) updates on cancer recurrence, morbidity and mortality in participating families, and c) biospecimens, including plasma, lymphocytes, serum, DNA, Guthrie cards or buccal smears, and paraffin blocks of tumor tissue. For further information on these registries, contact the CFR Web site (http://epi.grants.cancer.gov/BCFR) or (301) 496-9600. NCI - Specimen Resource Locator The NCI Specimen Resource Locator (http://cancer.gov/specimens) is a database that helps researchers locate specimens for research. The database includes resources such as tissue banks and tissue procurement systems with access to normal, benign, precancerous, and/or cancerous human tissue covering a wide variety of organ sites. Researchers specify the types of specimens, number of cases, preservation methods, and associated data they require. The Locator will search the database and return a list of tissue resources most likely to meet their requirements. When no match is obtained, the researcher is referred to the NCI Tissue Expediter [(301) 496-7147; e-mail: tissexp@mail.nih.gov]. The Tissue Expediter is a scientist who can help match researchers with appropriate resources or identify appropriate collaborators when those are necessary. NIDDK - Biologic Samples from Diabetic Study Foundation A portion (1/3) of all stored nonrenewable samples (plasma, serum, urine) from subjects enrolled in the Diabetes Control and Complications Trial (DCCT) is available for use by the scientific community to address questions for which these samples may be invaluable. Announcements for using this resource appear in the NIH Guide for Grants and Contracts periodically. Inquiries may be addressed to: Catherine C. Cowie, Ph.D., Director, Diabetes Epidemiology Program, NIDDK, 6707 Democracy Blvd., Room 691, MSC 5460, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892-5460. Phone: (301) 594-8804; fax: (301) 480-3503; e-mail: cowiec@extra.niddk.nih.gov. NIDDK - NIDDK Central Repositories (Diabetes Prevention Study) The NIDDK Central Repositories have selected biosamples from the DPT-1 (The Diabetes Prevention Type 1) study that are available to qualified investigators through an application process. These samples are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. Information about how to apply for these materials can be obtained from the NIDDK Central Repositories by contacting Ms. Helen Ray of RTI, 1-919-316-3418, or hmp@rti.org. Direct scientific-technical inquiry to the Project Officer of the NIDDK Central Repositories, Dr. Rebekah Rasooly, at phone: (301) 594-6007; e-mail: rr185i@nih.gov. Visit the Repositories Web site at http://www.niddkrepository.org. NICHD - Brain and Tissue Bank for Developmental Disorders The purpose of the Bank is to collect, preserve, and distribute human tissues to investigators interested in autism and developmental disorders; normal tissues may be available for other research purposes. Further information can be obtained at www.btbank.org. The contact persons are H. Ron Zielke or Sally Wisniewsky, University of Maryland (1-800-847-1539), and Carol Petito or Stephanie Lojko, University of Miami (1-800-592-7246). NICHD - Reproductive Tissue Sample Repository (RTSaR) The Reproductive Tissue Sample Repository (RTSaR) is a virtual repository with online tissue sample acquisition capabilities. The RTSaR provides investigators with real-time access to human and nonhuman primate tissue and fluid inventories from four tissue bank facilities that are supported through the Specialized Cooperative Centers Program in Reproduction Research. The tissue banks are located at the University of California, San Diego (human ovary bank), Stanford University (human endometrium and DNA bank), Johns Hopkins University (male reproductive tissues and fluids), and the Oregon National Primate Research Center (nonhuman primate tissues). The web site for the RTSaR is https://rtsar.nichd.nih.gov/rtsar/login. If you wish to access the RTSaR, you can request an id and password to access the system by contacting the network administrator at RTSaR@mail.nih.gov. Once you access the system, contact information for each bank is provided. Access is open to all investigators living in North America who are supported by research and research training grants from the NIH. One id and password will be provided to each principal investigator that can be utilized by any person working in the P.I.’s laboratory, or, in the case of institutional training grants (T32) and institutional career development award programs (K12), any person supported by the aforementioned awards. NCRR - Human Tissues and Organs Resource (HTOR) The Human Tissues and Organs Resource (HTOR) cooperative agreement supports a procurement network developed by the National Disease Research Interchange (NDRI), a not-for-profit organization. By collaborating with various medical centers, hospitals, pathology services, eye banks, tissue banks, and organ procurement organizations, HTOR provides a wide variety of human tissues and organs—both diseased and normal—to researchers for laboratory studies. Such samples include tissues from the central nervous system and brain, cardiovascular system, endocrine system, eyes, bone, and cartilage. For further information, consult the NDRI Web site (www.ndri.com) or contact Dr. John T. Lonsdale at NDRI, 8 Penn Center, 8th Floor, 1628 JFK Boulevard, Philadelphia, PA 19103. Phone: (800) 222-6374, ext. 271; fax: (215) 557-7154; e-mail: jlonsdale@ndriresource.org. The NDRI Web site is http://www.ndri.com. NCRR - Islet Cell Resource (ICR) With support from NCRR, 10 Islet Cell Resource (ICR) centers isolate, purify, and characterize human pancreatic islets for subsequent transplantation into patients with type I diabetes. The ICR centers procure whole pancreata and acquire relevant data about donors; improve islet isolation and purification techniques; distribute islets for use in approved clinical protocols; and perfect the methods of storage and shipping. In this way, the centers optimize the viability, function, and availability of islets and help clinical researchers capitalize on the recently reported successes in islet transplantation. Information on submitting requests for islet cells can be obtained from Mr. John Kaddis, ICR Coordinating Center Project Manager, City of Hope National Medical Center, 1500 E. Duarte Road, Duarte, California 91010. Phone (626) 359-8111, ext. 63377; fax: (626) 471-7106; e-mail: jkaddis@coh.org. The Coordinating Center hosts a Web site at http://icr.coh.org. NIA - SWAN Repository (longitudinal, multiethnic study of women at midlife including the menopausal transition) The SWAN Repository is a biologic specimen bank of the Study of Women’s Health Across the Nation (SWAN). The SWAN cohort was recruited in 1996/1997 and consists of 3302 African-American, Caucasian, Chinese, Hispanic, and Japanese women. The SWAN Repository contains more than 350,000 blood and urine specimens generated from the study participants’ annual visits (8 visits to date), at which time medical and health history, psychosocial measures, biological measures, and anthropometric data were and are being collected. In addition, a subset of the participants are providing urine samples, collected daily over the length of one menstrual cycle, each year. More than 900,000 of these samples are in the SWAN Repository and are available to researchers who wish to study the midlife and menopausal transition. Additionally, a DNA sample repository is also available and includes DNA as well as transformed B-lymphoblastoid cell lines from more than 1800 of the participants. To learn more about the SWAN Repository and how to apply to use SWAN Repository specimens, contact the Web site at http://www. swanrepository.com or Dr. MaryFran Sowers, University of Michigan, School of Public Health, Epidemiology Dept., (734) 936-3892; e-mail: mfsowers@umich.edu. Human and Animal Cell and Biologic Reagent Resources NIDDK - National Hormone and Peptide Program The National Hormone and Peptide Program (NHPP) offers peptide hormones and their antisera, tissues (rat hypothalami), and miscellaneous reagents to qualified investigators. These reagents are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. These materials can be obtained from Dr. A. F. Parlow of the Harbor-UCLA Medical Center, Research and Education Institute, Torrance, CA. A more complete description of resources within this program is provided in The Endocrine Society journals. Direct scientific-technical inquiry to NHPP Scientific Director, Dr. Al Parlow, at phone: (310) 222-3537; fax: (310) 222-3432; e-mail: parlow@humc.edu. Visit the NHPP Web site at http://www.humc.edu/hormones. NICHD - National Hormone and Pituitary Program (see NIDDK listing) Following is a list of reagents currently available through the resources of NICHD: Androgen receptor and peptide antigen Recombinant monkey (cynomolgus) and baboon luteinizing hormone and follicle-stimulating hormone and antisera. NIA - Aging Cell Bank To facilitate aging research on cells in culture, the NIA provides support for the Aging Cell Bank located at the Coriell Institute for Medical Research in Camden, NJ. The Aged Cell Bank provides fibroblast, lymphoblastoid, and differentiated cell lines from a wide range of human age-related conditions and other mammalian species, as well as DNA from a limited subset of cell lines. For further information, the Aged Cell Bank catalog can be accessed at http://locus.umdnj.edu/nia or contact Dr. Donald Coppock at 1-800-752-3805. NCRR - Various Cell Repositories NCRR maintains the following cell repository resources: National Cell Culture Center, National Stem Cell Resource, and the Yeast Genetic Stock Center. Further information regarding these resources may be obtained through the NCRR Web site at: www.ncrr.nih.gov/ncrrprog/cmpdir/BIOLOG.asp. Animal Resources NIA - Aging Rodent Resources NIA maintains both rat and mouse colonies for use by the scientific community. The animals available range in age from 1 to 36 months. A repository of fresh-frozen tissue from the NIA aged rodent colonies is stocked with tissue from mouse and rat strains, including caloric-restricted BALB/c mice. The NIA also maintains a colony of calorically restricted rodents of selected genotypes, which are available to the scientific community. For further information, please refer to the Aged Rodent information handbook at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentColoniesHandbook/ or contact the Office of Biological Resources and Resource Development order desk. Phone: (301) 496-0181; fax: (301) 402-5597; e-mail: rodents@nia.nih.gov. NIA - Aged Rodent Tissue Bank The rodent tissue bank contains flash-frozen tissues from rodents in the NIA aged rodent colonies. Tissue is collected from rodents at 4 or 5 age points throughout the lifespan. Tissue arrays are also available. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentTissueBankHandbook/. NCRR - Mutant Mouse Regional Resource Centers (MMRRC) The Mutant Mouse Regional Resource Center (MMRRC) Program consists of centers that collectively operate as a one-stop shop to serve the biomedical research community. Investigators who have created select mutant mouse models may donate their models to an MMRRC for broad dissemination to other investigators who request them for noncommercial research investigations related to human health, disease, and treatments. The NCRR Division of Comparative Medicine (DCM) supports the MMRRCs, which are electronically linked through the MMRRC Informatics Coordinating Center (ICC) to function as one facility. The ICC, located at The Jackson Laboratory in Bar Harbor, ME, provides database and other informatics support to the MMRRC to give the research community a single entry point to the program. Further information can be obtained from the Web site at http://www.mmrrc.org, or from Franziska Grieder, D.V.M., Ph.D., Division of Comparative Medicine, NCRR. Phone (301) 435-0744; fax: (301) 480-3819; e-mail: griederf@ncrr.nih.gov. NCRR - Induced Mutant Mouse Resource (IMR) The Induced Mutant Mouse Resource (IMR) at The Jackson Laboratory provides researchers with genetically engineered mice (transgenic, targeted mutant, retroviral insertional mutant, and chemically induced mutant mice). The function of the IMR is to select, import, cryopreserve, maintain, and distribute these important strains of mice to the research community. To improve their value for research, the IMR also undertakes genetic development of stocks, such as transferring mutant genes or transgenes to defined genetic backgrounds and combining transgenes and/or targeted mutations to create new mouse models for research. Over 800 mutant stocks have been accepted by the IMR. Current holdings include models for research on cancer, immunological and inflammatory diseases, neurological diseases and behavioral disorders, cardiovascular diseases, developmental disorders, metabolic and other diseases, reporter (e.g. GFP) and recombinase (e.g. cre/loxP) strains. About 8 strains a month are being added to the IMR holdings. A list of all strains may be obtained from the IMR Web site: www.jax.org/resources/documents/imr/. Online submission forms are also available on that site. All mice can be ordered by calling The Jackson Laboratory’s Customer Service Department at 1-800-422-MICE or (207) 288-5845 or by faxing (207) 288-6150. NIDDK - Mouse Metabolic Phenotyping Centers The mission of the Mouse Metabolic Phenotyping Centers is to provide the scientific community with standardized, high-quality metabolic and physiologic phenotyping services for mouse models of diabetes, diabetic complications, obesity, and related disorders. Researchers can ship mice to one of the four Centers (University of Cincinnati, University of Texas Southwestern Medical Center, Vanderbilt University, and Yale University) and obtain on a fee-for-service basis a range of complex exams used to characterize mouse metabolism, blood composition, energy balance, eating and exercise, organ function and morphology, physiology, and histology. Many tests are done in living animals and are designed to elucidate the subtle hallmarks of metabolic disease. Information, including a complete list of available tests, can be found at www.mmpc.org, or contact Dr. Maren R. Laughlin, NIDDK, at (301) 594-8802; e-mail: Maren.Laughlin@nih.gov; or Dr. Kristin Abraham, NIDDK, at (301) 451-8048; e-mail: abrahamk@extra.niddk.nih.gov. NCRR - National Primate Research Centers (NPRCs) National Primate Research Centers (NPRCs) are a network of eight highly specialized facilities for nonhuman primates (NHP) research. Funded by grants through NCRR’s Division of Comparative Medicine (DCM), each center, staffed with experienced research and support staff, provides the appropriate research environment to foster the development of NHP models of human health and disease for biomedical investigations. The NPRCs are affiliated with academic institutions and are accessible to eligible biomedical and behavioral investigators supported by research project grants from the National Institutes of Health and other sources. Further information may be obtained from the notice, Procedures for Accessing Regional Primate Research Centers, published in the NIH Guide for Grants and Contracts at http://grants2.nih.gov/grants/guide/notice-files/not97-014.html, or from John Harding, Ph.D., National Primate Research Centers and AIDS Animal Models Program, Division of Comparative Medicine, NCRR. Phone: (301) 435-0744; fax: (301) 480-3819; e-mail: hardingj@mail.nih.gov. NIA - Nonhuman Primates, Aging Set-Aside Colony NIA maintains approximately 200 nonhuman primates (M. mulatta) at four National Primate Research Centers (see above) for conducting research on aging. These animals range in age from 18 to 35 years. While these animals are predominantly reserved for non-invasive research, exceptions can be made to this policy. For further information, please contact Dr. Nancy Nadon, Office of Biological Resources and Resource Development, NIA. Phone: (301) 402-7744; fax: (301) 402-0010; e-mail: nadonn@nia.nih.gov. NIA - Nonhuman Primate (NHP) Tissue Bank and Aging Database The NIA developed two new resources to facilitate research in the NHP model. The NHP tissue bank contains fresh-frozen and fixed tissue donated by primate centers around the country. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/NHPTissueBankHandbook.htm.The Primate Aging Database provides an internet accessible database with data from thousands of primates around the country. It can be used to investigate the effect of age on a variety of parameters, predominantly blood chemistry and husbandry measurements. The site is password protected. The URL is http://ipad.primate.wisc.edu. NIA - Obesity, Diabetes and Aging Animal Resource (USF-ODARC) The NIA supports a colony of aged rhesus macaques, many of which are obese and/or diabetic. This is a long-term colony of monkeys housed at the University of South Florida’s Obesity, Diabetes and Aging Research Center. They have been extensively and longitudinally characterized for general health variables, blood chemistry, food intake, and body weight. Diabetic monkeys are tested daily for urine glucose and ketone levels, and prediabetic monkeys are tested weekly. Data for some of the monkeys extend as far back as 15 years. This unique resource is available for collaborative studies. ODARC has a significant amount of stored tissue collected at necropsy and stored blood/plasma collected longitudinally. Serial blood collection or tissue collection at necropsy can also be performed prospectively. Testing and imaging can also be performed on the monkeys. Inquiries regarding collaborative studies using the ODARC colony should be directed to: Barbara C. Hansen, Ph.D., Director, Obesity, Diabetes and Aging Research Center, University of South Florida, All Children’s Hospital, 801 6th Street South #9340, St. Petersburg, FL 33701. Phone: (727) 767-6993; fax: (727) 767-7443; e-mail: bchansen@aol.com. NCRR - Various Animal Resources NCRR maintains the following animal resources: Animal Models and Genetic Stocks, Chimpanzee Biomedical Research Program, NIH Animal Genetic Resource, and the Specific Pathogen Free Macaque Breeding and Research Program. Further information regarding these and other resources may be obtained through the NCRR Web site at www.ncrr.nih.gov/comparative_med.asp. Miscellaneous Resources NCRR - National Gene Vector Laboratories (NGVLs) The National Gene Vector Laboratories (NGVLs), with core funding from NCRR, serve as a resource for researchers to obtain adequate quantities of clinical-grade vectors for human gene transfer protocols. The vector types include retrovirus, lentivirus, adenovirus, adeno-associated virus, herpes-virus, and DNA plasmids. The NGVLs consist of three vector production centers at: Baylor College of Medicine; City of Hope National Medical Center and Beckman Research Institute; and Indiana University, which also serves as the Coordinating Center for all the laboratories. Two additional laboratories conduct toxicology studies for NGVL-approved investigators. These laboratories are located at the Southern Research Institute and the University of Florida. Additional information about the process for requesting vector production and/or pharmacology/toxicology support should be directed to Ms. Lorraine Matheson, NGVL Project Coordinator, Indiana University School of Medicine. Phone: (317) 274-4519; fax: (317) 278-4518; e-mail: lrubin@iupui.edu. The NGVL Coordinating Center at Indiana University also hosts a Web site at http://www.ngvl.org. NCRR - General Clinical Research Centers (GCRCs) The General Clinical Research Centers (GCRCs) are a national network of 82 centers that provide optimal settings for medical investigators to conduct safe, controlled, state-of-the-art in-patient and out-patient studies of both children and adults. GCRCs also provide infrastructure and resources that support several career development opportunities. Investigators who have research project funding from the National Institutes of Health (NIH) and other peer-reviewed sources may apply to use GCRCs. Because the GCRCs support a full spectrum of patient-oriented scientific inquiry, researchers who use these centers can benefit from collaborative, multidisciplinary research opportunities. To request access to a GCRC facility, eligible investigators should initially contact a GCRC program director, listed in the National Center for Research Resources (NCRR) Clinical Research Resources Directory (www.ncrr.nih.gov/ncrrprog/clindir/crdirectory.asp). Further information can be obtained from Anthony R. Hayward, M.D., Director, Division for Clinical Research Resources, National Center for Research Resources at NIH. Phone: (301) 435-0790; e-mail: haywarda@ncrr.nih.gov.

Resources currently available to the scientific community that may be of interest for endocrinology research are described briefly here. More information is available through The Endocrine Society Home Page (http://www.endo-society.org) or the information provided below. Human Tissue and Biologic Specimen Resources NCI - Cooperative Human Tissue Network (CHTN) The NCI Cooperative Human Tissue Network (CHTN) provides normal, benign, precancerous, and cancerous human tissue to the scientific community for biomedical research. Specimens are collected according to the investigator’s individual protocol. Information provided with the specimens includes routine histopathologic and demographic data. The CHTN can also provide a variety of tissue microarrays. Contact the CHTN Web site at http://www-chtn.ims.nci.nih.gov, or 1-866-GO2-CHTN (1-866-462-2486). NCI - Cooperative Breast Cancer Tissue Resource (CBCTR) The NCI Cooperative Breast Cancer Tissue Resource (CBCTR) can provide researchers with access to formalin-fixed, paraffin-embedded primary breast cancer specimens, with associated pathologic, clinical, and outcome data. All specimens are evaluated for pathologic diagnosis by CBCTR pathologists using standard diagnostic criteria. The collection is particularly well suited for validation studies of diagnostic and prognostic markers. The CBCTR also makes available breast cancer tissue microarrays designed by NCI statisticians to provide high statistical power for studies of stage-specific markers of breast cancer. Contact CBCTR’s Web site at http://cbctr.nci.nih.gov, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770;marrouliss@imsweb.com. NCI - Cooperative Prostate Cancer Tissue Resource (CPCTR) The NCI Cooperative Prostate Cancer Tissue Resource (CPCTR) can provide access to over 4,000 cases of formalin-fixed, paraffin-embedded primary prostate cancer specimens, with associated pathology and clinical data. Fresh-frozen tissue is also available with limited clinical follow-up information. In addition, slides from prostate cancer tissue microarrays with associated pathology and clinical data are now available. Contact the CPCTR Web site at http://www.prostatetissues.org, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770;marrouliss@imsweb.com. NCI - AIDS and Cancer Specimen Resource (ACSR) The AIDS and Cancer Specimen Resource (ACSR) provides qualified researchers with tissue, cell, blood, and fluid specimens, as well as clinical data from patients with AIDS and cancer. The specimens and clinical data are available for research studies, particularly those that translate basic research findings to clinical application. Contact the ACSR Web site (http://acsr.ucsf.edu/) or Dr. Kishor Bhatia, (301) 496-7147;bhatiak@mail.nih.gov. NCI - Breast and Ovarian Cancer Family Registries (CFRs) The Breast and Ovarian CFRs facilitate and support interdisciplinary and population-based research on the identification and characterization of breast and ovarian cancer susceptibility genes, with particular emphasis on gene-gene and gene-environment interaction research. Available from the registries are: a) family history, epidemiologic and clinical data, b) updates on cancer recurrence, morbidity and mortality in participating families, and c) biospecimens, including plasma, lymphocytes, serum, DNA, Guthrie cards or buccal smears, and paraffin blocks of tumor tissue. For further information on these registries, contact the CFR Web site (http://epi.grants.cancer.gov/BCFR) or (301) 496-9600. NCI - Specimen Resource Locator The NCI Specimen Resource Locator (http://cancer.gov/specimens) is a database that helps researchers locate specimens for research. The database includes resources such as tissue banks and tissue procurement systems with access to normal, benign, precancerous, and/or cancerous human tissue covering a wide variety of organ sites. Researchers specify the types of specimens, number of cases, preservation methods, and associated data they require. The Locator will search the database and return a list of tissue resources most likely to meet their requirements. When no match is obtained, the researcher is referred to the NCI Tissue Expediter [(301) 496-7147;tissexp@mail.nih.gov]. The Tissue Expediter is a scientist who can help match researchers with appropriate resources or identify appropriate collaborators when those are necessary. NIDDK - Biologic Samples from Diabetic Study Foundation A portion (1/3) of all stored nonrenewable samples (plasma, serum, urine) from subjects enrolled in the Diabetes Control and Complications Trial (DCCT) is available for use by the scientific community to address questions for which these samples may be invaluable. Announcements for using this resource appear in the NIH Guide for Grants and Contracts periodically. Inquiries may be addressed to: Catherine C. Cowie, Ph.D., Director, Diabetes Epidemiology Program, NIDDK, 6707 Democracy Blvd., Room 691, MSC 5460, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892-5460. Phone: (301) 594-8804; fax: (301) 480-3503;cowiec@extra.niddk.nih.gov. NIDDK - NIDDK Central Repositories (Diabetes Prevention Study) The NIDDK Central Repositories have selected biosamples from the DPT-1 (The Diabetes Prevention Type 1) study that are available to qualified investigators through an application process. These samples are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. Information about how to apply for these materials can be obtained from the NIDDK Central Repositories by contacting Ms. Helen Ray of RTI, 1-919-316-3418, or hmp@rti.org. Direct scientific-technical inquiry to the Project Officer of the NIDDK Central Repositories, Dr. Rebekah Rasooly, at phone: (301) 594-6007;rr185i@nih.gov. Visit the Repositories Web site at http://www.niddkrepository.org. NICHD - Brain and Tissue Bank for Developmental Disorders The purpose of the Bank is to collect, preserve, and distribute human tissues to investigators interested in autism and developmental disorders; normal tissues may be available for other research purposes. Further information can be obtained at www.btbank.org. The contact persons are H. Ron Zielke or Sally Wisniewsky, University of Maryland (1-800-847-1539), and Carol Petito or Stephanie Lojko, University of Miami (1-800-592-7246). NICHD - Reproductive Tissue Sample Repository (RTSaR) The Reproductive Tissue Sample Repository (RTSaR) is a virtual repository with online tissue sample acquisition capabilities. The RTSaR provides investigators with real-time access to human and nonhuman primate tissue and fluid inventories from four tissue bank facilities that are supported through the Specialized Cooperative Centers Program in Reproduction Research. The tissue banks are located at the University of California, San Diego (human ovary bank), Stanford University (human endometrium and DNA bank), Johns Hopkins University (male reproductive tissues and fluids), and the Oregon National Primate Research Center (nonhuman primate tissues). The web site for the RTSaR is https://rtsar.nichd.nih.gov/rtsar/login. If you wish to access the RTSaR, you can request an id and password to access the system by contacting the network administrator at RTSaR@mail.nih.gov. Once you access the system, contact information for each bank is provided. Access is open to all investigators living in North America who are supported by research and research training grants from the NIH. One id and password will be provided to each principal investigator that can be utilized by any person working in the P.I.’s laboratory, or, in the case of institutional training grants (T32) and institutional career development award programs (K12), any person supported by the aforementioned awards. NCRR - Human Tissues and Organs Resource (HTOR) The Human Tissues and Organs Resource (HTOR) cooperative agreement supports a procurement network developed by the National Disease Research Interchange (NDRI), a not-for-profit organization. By collaborating with various medical centers, hospitals, pathology services, eye banks, tissue banks, and organ procurement organizations, HTOR provides a wide variety of human tissues and organs—both diseased and normal—to researchers for laboratory studies. Such samples include tissues from the central nervous system and brain, cardiovascular system, endocrine system, eyes, bone, and cartilage. For further information, consult the NDRI Web site (www.ndri.com) or contact Dr. John T. Lonsdale at NDRI, 8 Penn Center, 8th Floor, 1628 JFK Boulevard, Philadelphia, PA 19103. Phone: (800) 222-6374, ext. 271; fax: (215) 557-7154;jlonsdale@ndriresource.org. The NDRI Web site is http://www.ndri.com. NCRR - Islet Cell Resource (ICR) With support from NCRR, 10 Islet Cell Resource (ICR) centers isolate, purify, and characterize human pancreatic islets for subsequent transplantation into patients with type I diabetes. The ICR centers procure whole pancreata and acquire relevant data about donors; improve islet isolation and purification techniques; distribute islets for use in approved clinical protocols; and perfect the methods of storage and shipping. In this way, the centers optimize the viability, function, and availability of islets and help clinical researchers capitalize on the recently reported successes in islet transplantation. Information on submitting requests for islet cells can be obtained from Mr. John Kaddis, ICR Coordinating Center Project Manager, City of Hope National Medical Center, 1500 E. Duarte Road, Duarte, California 91010. Phone (626) 359-8111, ext. 63377; fax: (626) 471-7106;jkaddis@coh.org. The Coordinating Center hosts a Web site at http://icr.coh.org. NIA - SWAN Repository (longitudinal, multiethnic study of women at midlife including the menopausal transition) The SWAN Repository is a biologic specimen bank of the Study of Women’s Health Across the Nation (SWAN). The SWAN cohort was recruited in 1996/1997 and consists of 3302 African-American, Caucasian, Chinese, Hispanic, and Japanese women. The SWAN Repository contains more than 350,000 blood and urine specimens generated from the study participants’ annual visits (8 visits to date), at which time medical and health history, psychosocial measures, biological measures, and anthropometric data were and are being collected. In addition, a subset of the participants are providing urine samples, collected daily over the length of one menstrual cycle, each year. More than 900,000 of these samples are in the SWAN Repository and are available to researchers who wish to study the midlife and menopausal transition. Additionally, a DNA sample repository is also available and includes DNA as well as transformed B-lymphoblastoid cell lines from more than 1800 of the participants. To learn more about the SWAN Repository and how to apply to use SWAN Repository specimens, contact the Web site at http://www. swanrepository.com or Dr. MaryFran Sowers, University of Michigan, School of Public Health, Epidemiology Dept., (734) 936-3892;mfsowers@umich.edu. Human and Animal Cell and Biologic Reagent Resources NIDDK - National Hormone and Peptide Program The National Hormone and Peptide Program (NHPP) offers peptide hormones and their antisera, tissues (rat hypothalami), and miscellaneous reagents to qualified investigators. These reagents are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. These materials can be obtained from Dr. A. F. Parlow of the Harbor-UCLA Medical Center, Research and Education Institute, Torrance, CA. A more complete description of resources within this program is provided in The Endocrine Society journals. Direct scientific-technical inquiry to NHPP Scientific Director, Dr. Al Parlow, at phone: (310) 222-3537; fax: (310) 222-3432;parlow@humc.edu. Visit the NHPP Web site at http://www.humc.edu/hormones. NICHD - National Hormone and Pituitary Program (see NIDDK listing) Following is a list of reagents currently available through the resources of NICHD: Androgen receptor and peptide antigen Recombinant monkey (cynomolgus) and baboon luteinizing hormone and follicle-stimulating hormone and antisera. NIA - Aging Cell Bank To facilitate aging research on cells in culture, the NIA provides support for the Aging Cell Bank located at the Coriell Institute for Medical Research in Camden, NJ. The Aged Cell Bank provides fibroblast, lymphoblastoid, and differentiated cell lines from a wide range of human age-related conditions and other mammalian species, as well as DNA from a limited subset of cell lines. For further information, the Aged Cell Bank catalog can be accessed at http://locus.umdnj.edu/nia or contact Dr. Donald Coppock at 1-800-752-3805. NCRR - Various Cell Repositories NCRR maintains the following cell repository resources: National Cell Culture Center, National Stem Cell Resource, and the Yeast Genetic Stock Center. Further information regarding these resources may be obtained through the NCRR Web site at: www.ncrr.nih.gov/ncrrprog/cmpdir/BIOLOG.asp. Animal Resources NIA - Aging Rodent Resources NIA maintains both rat and mouse colonies for use by the scientific community. The animals available range in age from 1 to 36 months. A repository of fresh-frozen tissue from the NIA aged rodent colonies is stocked with tissue from mouse and rat strains, including caloric-restricted BALB/c mice. The NIA also maintains a colony of calorically restricted rodents of selected genotypes, which are available to the scientific community. For further information, please refer to the Aged Rodent information handbook at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentColoniesHandbook/ or contact the Office of Biological Resources and Resource Development order desk. Phone: (301) 496-0181; fax: (301) 402-5597;rodents@nia.nih.gov. NIA - Aged Rodent Tissue Bank The rodent tissue bank contains flash-frozen tissues from rodents in the NIA aged rodent colonies. Tissue is collected from rodents at 4 or 5 age points throughout the lifespan. Tissue arrays are also available. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentTissueBankHandbook/. NCRR - Mutant Mouse Regional Resource Centers (MMRRC) The Mutant Mouse Regional Resource Center (MMRRC) Program consists of centers that collectively operate as a one-stop shop to serve the biomedical research community. Investigators who have created select mutant mouse models may donate their models to an MMRRC for broad dissemination to other investigators who request them for noncommercial research investigations related to human health, disease, and treatments. The NCRR Division of Comparative Medicine (DCM) supports the MMRRCs, which are electronically linked through the MMRRC Informatics Coordinating Center (ICC) to function as one facility. The ICC, located at The Jackson Laboratory in Bar Harbor, ME, provides database and other informatics support to the MMRRC to give the research community a single entry point to the program. Further information can be obtained from the Web site at http://www.mmrrc.org, or from Franziska Grieder, D.V.M., Ph.D., Division of Comparative Medicine, NCRR. Phone (301) 435-0744; fax: (301) 480-3819;griederf@ncrr.nih.gov. NCRR - Induced Mutant Mouse Resource (IMR) The Induced Mutant Mouse Resource (IMR) at The Jackson Laboratory provides researchers with genetically engineered mice (transgenic, targeted mutant, retroviral insertional mutant, and chemically induced mutant mice). The function of the IMR is to select, import, cryopreserve, maintain, and distribute these important strains of mice to the research community. To improve their value for research, the IMR also undertakes genetic development of stocks, such as transferring mutant genes or transgenes to defined genetic backgrounds and combining transgenes and/or targeted mutations to create new mouse models for research. Over 800 mutant stocks have been accepted by the IMR. Current holdings include models for research on cancer, immunological and inflammatory diseases, neurological diseases and behavioral disorders, cardiovascular diseases, developmental disorders, metabolic and other diseases, reporter (e.g. GFP) and recombinase (e.g. cre/loxP) strains. About 8 strains a month are being added to the IMR holdings. A list of all strains may be obtained from the IMR Web site: www.jax.org/resources/documents/imr/. Online submission forms are also available on that site. All mice can be ordered by calling The Jackson Laboratory’s Customer Service Department at 1-800-422-MICE or (207) 288-5845 or by faxing (207) 288-6150. NIDDK - Mouse Metabolic Phenotyping Centers The mission of the Mouse Metabolic Phenotyping Centers is to provide the scientific community with standardized, high-quality metabolic and physiologic phenotyping services for mouse models of diabetes, diabetic complications, obesity, and related disorders. Researchers can ship mice to one of the four Centers (University of Cincinnati, University of Texas Southwestern Medical Center, Vanderbilt University, and Yale University) and obtain on a fee-for-service basis a range of complex exams used to characterize mouse metabolism, blood composition, energy balance, eating and exercise, organ function and morphology, physiology, and histology. Many tests are done in living animals and are designed to elucidate the subtle hallmarks of metabolic disease. Information, including a complete list of available tests, can be found at www.mmpc.org, or contact Dr. Maren R. Laughlin, NIDDK, at (301) 594-8802;Maren.Laughlin@nih.gov; or Dr. Kristin Abraham, NIDDK, at (301) 451-8048;abrahamk@extra.niddk.nih.gov. NCRR - National Primate Research Centers (NPRCs) National Primate Research Centers (NPRCs) are a network of eight highly specialized facilities for nonhuman primates (NHP) research. Funded by grants through NCRR’s Division of Comparative Medicine (DCM), each center, staffed with experienced research and support staff, provides the appropriate research environment to foster the development of NHP models of human health and disease for biomedical investigations. The NPRCs are affiliated with academic institutions and are accessible to eligible biomedical and behavioral investigators supported by research project grants from the National Institutes of Health and other sources. Further information may be obtained from the notice, Procedures for Accessing Regional Primate Research Centers, published in the NIH Guide for Grants and Contracts at http://grants2.nih.gov/grants/guide/notice-files/not97-014.html, or from John Harding, Ph.D., National Primate Research Centers and AIDS Animal Models Program, Division of Comparative Medicine, NCRR. Phone: (301) 435-0744; fax: (301) 480-3819;hardingj@mail.nih.gov. NIA - Nonhuman Primates, Aging Set-Aside Colony NIA maintains approximately 200 nonhuman primates (M. mulatta) at four National Primate Research Centers (see above) for conducting research on aging. These animals range in age from 18 to 35 years. While these animals are predominantly reserved for non-invasive research, exceptions can be made to this policy. For further information, please contact Dr. Nancy Nadon, Office of Biological Resources and Resource Development, NIA. Phone: (301) 402-7744; fax: (301) 402-0010;nadonn@nia.nih.gov. NIA - Nonhuman Primate (NHP) Tissue Bank and Aging Database The NIA developed two new resources to facilitate research in the NHP model. The NHP tissue bank contains fresh-frozen and fixed tissue donated by primate centers around the country. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/NHPTissueBankHandbook.htm.The Primate Aging Database provides an internet accessible database with data from thousands of primates around the country. It can be used to investigate the effect of age on a variety of parameters, predominantly blood chemistry and husbandry measurements. The site is password protected. The URL is http://ipad.primate.wisc.edu. NIA - Obesity, Diabetes and Aging Animal Resource (USF-ODARC) The NIA supports a colony of aged rhesus macaques, many of which are obese and/or diabetic. This is a long-term colony of monkeys housed at the University of South Florida’s Obesity, Diabetes and Aging Research Center. They have been extensively and longitudinally characterized for general health variables, blood chemistry, food intake, and body weight. Diabetic monkeys are tested daily for urine glucose and ketone levels, and prediabetic monkeys are tested weekly. Data for some of the monkeys extend as far back as 15 years. This unique resource is available for collaborative studies. ODARC has a significant amount of stored tissue collected at necropsy and stored blood/plasma collected longitudinally. Serial blood collection or tissue collection at necropsy can also be performed prospectively. Testing and imaging can also be performed on the monkeys. Inquiries regarding collaborative studies using the ODARC colony should be directed to: Barbara C. Hansen, Ph.D., Director, Obesity, Diabetes and Aging Research Center, University of South Florida, All Children’s Hospital, 801 6th Street South #9340, St. Petersburg, FL 33701. Phone: (727) 767-6993; fax: (727) 767-7443;bchansen@aol.com. NCRR - Various Animal Resources NCRR maintains the following animal resources: Animal Models and Genetic Stocks, Chimpanzee Biomedical Research Program, NIH Animal Genetic Resource, and the Specific Pathogen Free Macaque Breeding and Research Program. Further information regarding these and other resources may be obtained through the NCRR Web site at www.ncrr.nih.gov/comparative_med.asp. Miscellaneous Resources NCRR - National Gene Vector Laboratories (NGVLs) The National Gene Vector Laboratories (NGVLs), with core funding from NCRR, serve as a resource for researchers to obtain adequate quantities of clinical-grade vectors for human gene transfer protocols. The vector types include retrovirus, lentivirus, adenovirus, adeno-associated virus, herpes-virus, and DNA plasmids. The NGVLs consist of three vector production centers at: Baylor College of Medicine; City of Hope National Medical Center and Beckman Research Institute; and Indiana University, which also serves as the Coordinating Center for all the laboratories. Two additional laboratories conduct toxicology studies for NGVL-approved investigators. These laboratories are located at the Southern Research Institute and the University of Florida. Additional information about the process for requesting vector production and/or pharmacology/toxicology support should be directed to Ms. Lorraine Matheson, NGVL Project Coordinator, Indiana University School of Medicine. Phone: (317) 274-4519; fax: (317) 278-4518;lrubin@iupui.edu. The NGVL Coordinating Center at Indiana University also hosts a Web site at http://www.ngvl.org. NCRR - General Clinical Research Centers (GCRCs) The General Clinical Research Centers (GCRCs) are a national network of 82 centers that provide optimal settings for medical investigators to conduct safe, controlled, state-of-the-art in-patient and out-patient studies of both children and adults. GCRCs also provide infrastructure and resources that support several career development opportunities. Investigators who have research project funding from the National Institutes of Health (NIH) and other peer-reviewed sources may apply to use GCRCs. Because the GCRCs support a full spectrum of patient-oriented scientific inquiry, researchers who use these centers can benefit from collaborative, multidisciplinary research opportunities. To request access to a GCRC facility, eligible investigators should initially contact a GCRC program director, listed in the National Center for Research Resources (NCRR) Clinical Research Resources Directory (www.ncrr.nih.gov/ncrrprog/clindir/crdirectory.asp). Further information can be obtained from Anthony R. Hayward, M.D., Director, Division for Clinical Research Resources, National Center for Research Resources at NIH. Phone: (301) 435-0790;haywarda@ncrr.nih.gov.

Resources currently available to the scientific community that may be of interest for endocrinology research are described briefly here. More information is available through The Endocrine Society Home Page (http://www.endo-society.org) or the information provided below. HUMAN TISSUE AND BIOLOGIC SPECIMEN RESOURCES NCI - Cooperative Human Tissue Network (CHTN) The NCI Cooperative Human Tissue Network (CHTN) provides normal, benign, precancerous, and cancerous human tissue to the scientific community for biomedical research. Specimens are collected according to the investigator’s individual protocol. Information provided with the specimens includes routine histopathologic and demographic data. The CHTN can also provide a variety of tissue microarrays. Contact the CHTN Web site at http://www-chtn.ims.nci.nih.gov, or 1-866-GO2-CHTN (1-866-462-2486). NCI - Cooperative Breast Cancer Tissue Resource (CBCTR) The NCI Cooperative Breast Cancer Tissue Resource (CBCTR) can provide researchers with access to formalin-fixed, paraffin-embedded primary breast cancer specimens, with associated pathologic, clinical, and outcome data. All specimens are evaluated for pathologic diagnosis by CBCTR pathologists using standard diagnostic criteria. The collection is particularly well suited for validation studies of diagnostic and prognostic markers. The CBCTR also makes available breast cancer tissue microarrays designed by NCI statisticians to provide high statistical power for studies of stage-specific markers of breast cancer. Contact CBCTR’s Web site at http://cbctr.nci.nih.gov, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; email: marrouliss@imsweb.com. NCI - Cooperative Prostate Cancer Tissue Resource (CPCTR) The NCI Cooperative Prostate Cancer Tissue Resource (CPCTR) can provide access to over 4,000 cases of formalin-fixed, paraffin-embedded primary prostate cancer specimens, with associated pathology and clinical data. Fresh-frozen tissue is also available with limited clinical follow-up information. In addition, slides from prostate cancer tissue microarrays with associated pathology and clinical data are now available. Contact the CPCTR Web site at http://www.prostatetissues.org, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; email: marrouliss@imsweb.com. NCI - AIDS and Cancer Specimen Resource (ACSR) The AIDS and Cancer Specimen Resource (ACSR) provides qualified researchers with tissue, cell, blood, and fluid specimens, as well as clinical data from patients with AIDS and cancer. The specimens and clinical data are available for research studies, particularly those that translate basic research findings to clinical application. Contact the ACSR Web site (http://acsr.ucsf.edu/) or Dr. Kishor Bhatia, (301) 496-7147; email: bhatiak@mail.nih.gov. NCI - Breast and Ovarian Cancer Family Registries (CFRs) The Breast and Ovarian CFRs facilitate and support interdisciplinary and population-based research on the identification and characterization of breast and ovarian cancer susceptibility genes, with particular emphasis on gene-gene and gene-environment interaction research. Available from the registries are: a) family history, epidemiologic and clinical data, b) updates on cancer recurrence, morbidity and mortality in participating families, and c) biospecimens, including plasma, lymphocytes, serum, DNA, Guthrie cards or buccal smears, and paraffin blocks of tumor tissue. For further information on these registries, contact the CFR Web site (http://epi.grants.cancer.gov/BCFR) or (301) 496-9600. NCI - Specimen Resource Locator The NCI Specimen Resource Locator (http://cancer.gov/specimens) is a database that helps researchers locate specimens for research. The database includes resources such as tissue banks and tissue procurement systems with access to normal, benign, precancerous, and/or cancerous human tissue covering a wide variety of organ sites. Researchers specify the types of specimens, number of cases, preservation methods, and associated data they require. The Locator will search the database and return a list of tissue resources most likely to meet their requirements. When no match is obtained, the researcher is referred to the NCI Tissue Expediter [(301) 496-7147; email: tissexp@mail.nih.gov]. The Tissue Expediter is a scientist who can help match researchers with appropriate resources or identify appropriate collaborators when those are necessary. NIDDK - Biologic Samples from Diabetic Study Foundation A portion (1/3) of all stored nonrenewable samples (plasma, serum, urine) from subjects enrolled in the Diabetes Control and Complications Trial (DCCT) is available for use by the scientific community to address questions for which these samples may be invaluable. Announcements for using this resource appear in the NIH Guide for Grants and Contracts periodically. Inquiries may be addressed to: Catherine C. Cowie, Ph.D., Director, Diabetes Epidemiology Program, NIDDK, 6707 Democracy Blvd., Room 691, MSC 5460, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892-5460. Phone: (301) 594-8804; fax: (301) 480-3503; email: cowiec@extra.niddk.nih.gov. NIDDK - NIDDK Central Repositories (Diabetes Prevention Study) The NIDDK Central Repositories have selected biosamples from the DPT-1 (The Diabetes Prevention Type 1) study that are available to qualified investigators through an application process. These samples are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. Information about how to apply for these materials can be obtained from the NIDDK Central Repositories by contacting Ms. Helen Ray of RTI, 1-919-316-3418, or hmp@rti.org. Direct scientific-technical inquiry to the Project Officer of the NIDDK Central Repositories, Dr. Rebekah Rasooly, at phone: (301) 594-6007; email: rr185i@nih.gov. Visit the Repositories Web site at http://www.niddkrepository.org. NICHD - Brain and Tissue Bank for Developmental Disorders The purpose of the Bank is to collect, preserve, and distribute human tissues to investigators interested in autism and developmental disorders; normal tissues may be available for other research purposes. Further information can be obtained at www.btbank.org. The contact persons are H. Ron Zielke or Sally Wisniewsky, University of Maryland (1-800-847-1539), and Carol Petito or Stephanie Lojko, University of Miami (1-800-592-7246). NICHD - Reproductive Tissue Sample Repository (RTSaR) The Reproductive Tissue Sample Repository (RTSaR) is a virtual repository with online tissue sample acquisition capabilities. The RTSaR provides investigators with real-time access to human and nonhuman primate tissue and fluid inventories from four tissue bank facilities that are supported through the Specialized Cooperative Centers Program in Reproduction Research. The tissue banks are located at the University of California, San Diego (human ovary bank), Stanford University (human endometrium and DNA bank), Johns Hopkins University (male reproductive tissues and fluids), and the Oregon National Primate Research Center (nonhuman primate tissues). The web site for the RTSaR is https://rtsar.nichd.nih.gov/rtsar/login. If you wish to access the RTSaR, you can request an id and password to access the system by contacting the network administrator at RTSaR@mail.nih.gov. Once you access the system, contact information for each bank is provided. Access is open to all investigators living in North America who are supported by research and research training grants from the NIH. One id and password will be provided to each principal investigator that can be utilized by any person working in the P.I.’s laboratory, or, in the case of institutional training grants (T32) and institutional career development award programs (K12), any person supported by the aforementioned awards. NCRR - Human Tissues and Organs Resource (HTOR) The Human Tissues and Organs Resource (HTOR) cooperative agreement supports a procurement network developed by the National Disease Research Interchange (NDRI), a not-for-profit organization. By collaborating with various medical centers, hospitals, pathology services, eye banks, tissue banks, and organ procurement organizations, HTOR provides a wide variety of human tissues and organs—both diseased and normal—to researchers for laboratory studies. Such samples include tissues from the central nervous system and brain, cardiovascular system, endocrine system, eyes, bone, and cartilage. For further information, consult the NDRI Web site (www.ndri.com) or contact Dr. John T. Lonsdale at NDRI, 8 Penn Center, 8th Floor, 1628 JFK Boulevard, Philadelphia, PA 19103. Phone: (800) 222-6374, ext. 271; fax: (215) 557-7154; email: jlonsdale@ndriresource.org. The NDRI Web site is http://www.ndri.com. NCRR - Islet Cell Resource (ICR) With support from NCRR, 10 Islet Cell Resource (ICR) centers isolate, purify, and characterize human pancreatic islets for subsequent transplantation into patients with type I diabetes. The ICR centers procure whole pancreata and acquire relevant data about donors; improve islet isolation and purification techniques; distribute islets for use in approved clinical protocols; and perfect the methods of storage and shipping. In this way, the centers optimize the viability, function, and availability of islets and help clinical researchers capitalize on the recently reported successes in islet transplantation. Information on submitting requests for islet cells can be obtained from Mr. John Kaddis, ICR Coordinating Center Project Manager, City of Hope National Medical Center, 1500 E. Duarte Road, Duarte, California 91010. Phone (626) 359-8111, ext. 63377; fax: (626) 471-7106; email: jkaddis@coh.org. The Coordinating Center hosts a Web site at http://icr.coh.org. NIA - SWAN Repository (longitudinal, multiethnic study of women at midlife including the menopausal transition) The SWAN Repository is a biologic specimen bank of the Study of Women’s Health Across the Nation (SWAN). The SWAN cohort was recruited in 1996/1997 and consists of 3302 African-American, Caucasian, Chinese, Hispanic, and Japanese women. The SWAN Repository contains more than 350,000 blood and urine specimens generated from the study participants’ annual visits (8 visits to date), at which time medical and health history, psychosocial measures, biological measures, and anthropometric data were and are being collected. In addition, a subset of the participants are providing urine samples, collected daily over the length of one menstrual cycle, each year. More than 900,000 of these samples are in the SWAN Repository and are available to researchers who wish to study the midlife and menopausal transition. Additionally, a DNA sample repository is also available and includes DNA as well as transformed B-lymphoblastoid cell lines from more than 1800 of the participants. To learn more about the SWAN Repository and how to apply to use SWAN Repository specimens, contact the Web site at http://www. swanrepository.com or Dr. MaryFran Sowers, University of Michigan, School of Public Health, Epidemiology Dept., (734) 936-3892; email: mfsowers@umich.edu. HUMAN AND ANIMAL CELL AND BIOLOGIC REAGENT RESOURCES NIDDK - National Hormone and Peptide Program The National Hormone and Peptide Program (NHPP) offers peptide hormones and their antisera, tissues (rat hypothalami), and miscellaneous reagents to qualified investigators. These reagents are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. These materials can be obtained from Dr. A. F. Parlow of the Harbor-UCLA Medical Center, Research and Education Institute, Torrance, CA. A more complete description of resources within this program is provided in The Endocrine Society journals. Direct scientific-technical inquiry to NHPP Scientific Director, Dr. Al Parlow, at phone: (310) 222-3537; fax: (310) 222-3432; email: parlow@humc.edu. Visit the NHPP Web site at http://www.humc.edu/hormones. NICHD - National Hormone and Pituitary Program (see NIDDK listing) Following is a list of reagents currently available through the resources of NICHD: Androgen receptor and peptide antigen Recombinant monkey (cynomolgus) and baboon luteinizing hormone and follicle-stimulating hormone and antisera. NIA - Aging Cell Bank To facilitate aging research on cells in culture, the NIA provides support for the Aging Cell Bank located at the Coriell Institute for Medical Research in Camden, NJ. The Aged Cell Bank provides fibroblast, lymphoblastoid, and differentiated cell lines from a wide range of human age-related conditions and other mammalian species, as well as DNA from a limited subset of cell lines. For further information, the Aged Cell Bank catalog can be accessed at http://locus.umdnj.edu/nia or contact Dr. Donald Coppock at 1-800-752-3805. NCRR - Various Cell Repositories NCRR maintains the following cell repository resources: National Cell Culture Center, National Stem Cell Resource, and the Yeast Genetic Stock Center. Further information regarding these resources may be obtained through the NCRR Web site at: www.ncrr.nih.gov/ncrrprog/cmpdir/BIOLOG.asp. ANIMAL RESOURCES NIA - Aging Rodent Resources NIA maintains both rat and mouse colonies for use by the scientific community. The animals available range in age from 1 to 36 months. A repository of fresh-frozen tissue from the NIA aged rodent colonies is stocked with tissue from mouse and rat strains, including caloric-restricted BALB/c mice. The NIA also maintains a colony of calorically restricted rodents of selected genotypes, which are available to the scientific community. For further information, please refer to the Aged Rodent information handbook at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentColoniesHandbook/ or contact the Office of Biological Resources and Resource Development order desk. Phone: (301) 496-0181; fax: (301) 402-5597; email: rodents@nia.nih.gov. NIA - Aged Rodent Tissue Bank The rodent tissue bank contains flash-frozen tissues from rodents in the NIA aged rodent colonies. Tissue is collected from rodents at 4 or 5 age points throughout the lifespan. Tissue arrays are also available. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentTissueBankHandbook/. NCRR - Mutant Mouse Regional Resource Centers (MMRRC) The Mutant Mouse Regional Resource Center (MMRRC) Program consists of centers that collectively operate as a one-stop shop to serve the biomedical research community. Investigators who have created select mutant mouse models may donate their models to an MMRRC for broad dissemination to other investigators who request them for noncommercial research investigations related to human health, disease, and treatments. The NCRR Division of Comparative Medicine (DCM) supports the MMRRCs, which are electronically linked through the MMRRC Informatics Coordinating Center (ICC) to function as one facility. The ICC, located at The Jackson Laboratory in Bar Harbor, ME, provides database and other informatics support to the MMRRC to give the research community a single entry point to the program. Further information can be obtained from the Web site at http://www.mmrrc.org, or from Franziska Grieder, D.V.M., Ph.D., Division of Comparative Medicine, NCRR. Phone (301) 435-0744; fax: (301) 480-3819; email: griederf@ncrr.nih.gov. NCRR - Induced Mutant Mouse Resource (IMR) The Induced Mutant Mouse Resource (IMR) at The Jackson Laboratory provides researchers with genetically engineered mice (transgenic, targeted mutant, retroviral insertional mutant, and chemically induced mutant mice). The function of the IMR is to select, import, cryopreserve, maintain, and distribute these important strains of mice to the research community. To improve their value for research, the IMR also undertakes genetic development of stocks, such as transferring mutant genes or transgenes to defined genetic backgrounds and combining transgenes and/or targeted mutations to create new mouse models for research. Over 800 mutant stocks have been accepted by the IMR. Current holdings include models for research on cancer, immunological and inflammatory diseases, neurological diseases and behavioral disorders, cardiovascular diseases, developmental disorders, metabolic and other diseases, reporter (e.g. GFP) and recombinase (e.g. cre/loxP) strains. About 8 strains a month are being added to the IMR holdings. A list of all strains may be obtained from the IMR Web site: www.jax.org/resources/documents/imr/. Online submission forms are also available on that site. All mice can be ordered by calling The Jackson Laboratory’s Customer Service Department at 1-800-422-MICE or (207) 288-5845 or by faxing (207) 288-6150. NIDDK - Mouse Metabolic Phenotyping Centers The mission of the Mouse Metabolic Phenotyping Centers is to provide the scientific community with standardized, high-quality metabolic and physiologic phenotyping services for mouse models of diabetes, diabetic complications, obesity, and related disorders. Researchers can ship mice to one of the four Centers (University of Cincinnati, University of Texas Southwestern Medical Center, Vanderbilt University, and Yale University) and obtain on a fee-for-service basis a range of complex exams used to characterize mouse metabolism, blood composition, energy balance, eating and exercise, organ function and morphology, physiology, and histology. Many tests are done in living animals and are designed to elucidate the subtle hallmarks of metabolic disease. Information, including a complete list of available tests, can be found at www.mmpc.org, or contact Dr. Maren R. Laughlin, NIDDK, at (301) 594-8802; email: Maren.Laughlin@nih.gov; or Dr. Kristin Abraham, NIDDK, at (301) 451-8048; email: abrahamk@extra.niddk.nih.gov. NCRR - National Primate Research Centers (NPRCs) National Primate Research Centers (NPRCs) are a network of eight highly specialized facilities for nonhuman primates (NHP) research. Funded by grants through NCRR’s Division of Comparative Medicine (DCM), each center, staffed with experienced research and support staff, provides the appropriate research environment to foster the development of NHP models of human health and disease for biomedical investigations. The NPRCs are affiliated with academic institutions and are accessible to eligible biomedical and behavioral investigators supported by research project grants from the National Institutes of Health and other sources. Further information may be obtained from the notice, Procedures for Accessing Regional Primate Research Centers, published in the NIH Guide for Grants and Contracts at http://grants2.nih.gov/grants/guide/notice-files/not97-014.html, or from John Harding, Ph.D., National Primate Research Centers and AIDS Animal Models Program, Division of Comparative Medicine, NCRR. Phone: (301) 435-0744; fax: (301) 480-3819; email: hardingj@mail.nih.gov. NIA - Nonhuman Primates, Aging Set-Aside Colony NIA maintains approximately 200 nonhuman primates (M. mulatta) at four National Primate Research Centers (see above) for conducting research on aging. These animals range in age from 18 to 35 years. While these animals are predominantly reserved for non-invasive research, exceptions can be made to this policy. For further information, please contact Dr. Nancy Nadon, Office of Biological Resources and Resource Development, NIA. Phone: (301) 402-7744; fax: (301) 402-0010; email: nadonn@nia.nih.gov. NIA - Nonhuman Primate (NHP) Tissue Bank and Aging Database The NIA developed two new resources to facilitate research in the NHP model. The NHP tissue bank contains fresh-frozen and fixed tissue donated by primate centers around the country. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/NHPTissueBankHandbook.htm. The Primate Aging Database provides an internet accessible database with data from thousands of primates around the country. It can be used to investigate the effect of age on a variety of parameters, predominantly blood chemistry and husbandry measurements. The site is password protected. The URL is http://ipad.primate.wisc.edu. NIA - Obesity, Diabetes and Aging Animal Resource (USF-ODARC) The NIA supports a colony of aged rhesus macaques, many of which are obese and/or diabetic. This is a long-term colony of monkeys housed at the University of South Florida’s Obesity, Diabetes and Aging Research Center. They have been extensively and longitudinally characterized for general health variables, blood chemistry, food intake, and body weight. Diabetic monkeys are tested daily for urine glucose and ketone levels, and prediabetic monkeys are tested weekly. Data for some of the monkeys extend as far back as 15 years. This unique resource is available for collaborative studies. ODARC has a significant amount of stored tissue collected at necropsy and stored blood/plasma collected longitudinally. Serial blood collection or tissue collection at necropsy can also be performed prospectively. Testing and imaging can also be performed on the monkeys. Inquiries regarding collaborative studies using the ODARC colony should be directed to: Barbara C. Hansen, Ph.D., Director, Obesity, Diabetes and Aging Research Center, University of South Florida, All Children’s Hospital, 801 6th Street South #9340, St. Petersburg, FL 33701. Phone: (727) 767-6993; fax: (727) 767-7443; email: bchansen@aol.com. NCRR - Various Animal Resources NCRR maintains the following animal resources: Animal Models and Genetic Stocks, Chimpanzee Biomedical Research Program, NIH Animal Genetic Resource, and the Specific Pathogen Free Macaque Breeding and Research Program. Further information regarding these and other resources may be obtained through the NCRR Web site at www.ncrr.nih.gov/comparative_med.asp. MISCELLANEOUS RESOURCES NCRR - National Gene Vector Laboratories (NGVLs) The National Gene Vector Laboratories (NGVLs), with core funding from NCRR, serve as a resource for researchers to obtain adequate quantities of clinical-grade vectors for human gene transfer protocols. The vector types include retrovirus, lentivirus, adenovirus, adeno-associated virus, herpes-virus, and DNA plasmids. The NGVLs consist of three vector production centers at: Baylor College of Medicine; City of Hope National Medical Center and Beckman Research Institute; and Indiana University, which also serves as the Coordinating Center for all the laboratories. Two additional laboratories conduct toxicology studies for NGVL-approved investigators. These laboratories are located at the Southern Research Institute and the University of Florida. Additional information about the process for requesting vector production and/or pharmacology/toxicology support should be directed to Ms. Lorraine Matheson, NGVL Project Coordinator, Indiana University School of Medicine. Phone: (317) 274-4519; fax: (317) 278-4518; email: lrubin@iupui.edu. The NGVL Coordinating Center at Indiana University also hosts a Web site at http://www.ngvl.org. NCRR - General Clinical Research Centers (GCRCs) The General Clinical Research Centers (GCRCs) are a national network of 82 centers that provide optimal settings for medical investigators to conduct safe, controlled, state-of-the-art in-patient and out-patient studies of both children and adults. GCRCs also provide infrastructure and resources that support several career development opportunities. Investigators who have research project funding from the National Institutes of Health (NIH) and other peer-reviewed sources may apply to use GCRCs. Because the GCRCs support a full spectrum of patient-oriented scientific inquiry, researchers who use these centers can benefit from collaborative, multidisciplinary research opportunities. To request access to a GCRC facility, eligible investigators should initially contact a GCRC program director, listed in the National Center for Research Resources (NCRR) Clinical Research Resources Directory (www.ncrr.nih.gov/ncrrprog/clindir/crdirectory.asp). Further information can be obtained from Anthony R. Hayward, M.D., Director, Division for Clinical Research Resources, National Center for Research Resources at NIH. Phone: (301) 435-0790; email: haywarda@ncrr.nih.gov.

Resources currently available to the scientific community that may be of interest for endocrinology research are described briefly here. More information is available through The Endocrine Society Home Page (http://www.endo-society.org) or the information provided below. HUMAN TISSUE AND BIOLOGIC SPECIMEN RESOURCES NCI - Cooperative Human Tissue Network (CHTN) The NCI Cooperative Human Tissue Network (CHTN) provides normal, benign, precancerous, and cancerous human tissue to the scientific community for biomedical research. Specimens are collected according to the investigator’s individual protocol. Information provided with the specimens includes routine histopathologic and demographic data. The CHTN can also provide a variety of tissue microarrays. Contact the CHTN Web site at http://www-chtn.ims.nci.nih.gov, or 1-866-GO2-CHTN (1-866-462-2486). NCI - Cooperative Breast Cancer Tissue Resource (CBCTR) The NCI Cooperative Breast Cancer Tissue Resource (CBCTR) can provide researchers with access to formalin-fixed, paraffin-embedded primary breast cancer specimens, with associated pathologic, clinical, and outcome data. All specimens are evaluated for pathologic diagnosis by CBCTR pathologists using standard diagnostic criteria. The collection is particularly well suited for validation studies of diagnostic and prognostic markers. The CBCTR also makes available breast cancer tissue microarrays designed by NCI statisticians to provide high statistical power for studies of stage-specific markers of breast cancer. Contact CBCTR’s Web site at http://cbctr.nci.nih.gov, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com. NCI - Cooperative Prostate Cancer Tissue Resource (CPCTR) The NCI Cooperative Prostate Cancer Tissue Resource (CPCTR) can provide access to over 4,000 cases of formalin-fixed, paraffin-embedded primary prostate cancer specimens, with associated pathology and clinical data. Fresh-frozen tissue is also available with limited clinical follow-up information. In addition, slides from prostate cancer tissue microarrays with associated pathology and clinical data are now available. Contact the CPCTR Web site at http://www.prostatetissues.org, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com. NCI - AIDS and Cancer Specimen Resource (ACSR) The AIDS and Cancer Specimen Resource (ACSR) provides qualified researchers with tissue, cell, blood, and fluid specimens, as well as clinical data from patients with AIDS and cancer. The specimens and clinical data are available for research studies, particularly those that translate basic research findings to clinical application. Contact the ACSR Web site (http://acsr.ucsf.edu/) or Dr. Kishor Bhatia, (301) 496-7147; e-mail: bhatiak@mail.nih.gov. NCI - Breast and Ovarian Cancer Family Registries (CFRs) The Breast and Ovarian CFRs facilitate and support interdisciplinary and population-based research on the identification and characterization of breast and ovarian cancer susceptibility genes, with particular emphasis on gene-gene and gene-environment interaction research. Available from the registries are: a) family history, epidemiologic and clinical data, b) updates on cancer recurrence, morbidity and mortality in participating families, and c) biospecimens, including plasma, lymphocytes, serum, DNA, Guthrie cards or buccal smears, and paraffin blocks of tumor tissue. For further information on these registries, contact the CFR Web site (http://epi.grants.cancer.gov/BCFR) or (301) 496-9600. NCI - Specimen Resource Locator The NCI Specimen Resource Locator (http://cancer.gov/specimens) is a database that helps researchers locate specimens for research. The database includes resources such as tissue banks and tissue procurement systems with access to normal, benign, precancerous, and/or cancerous human tissue covering a wide variety of organ sites. Researchers specify the types of specimens, number of cases, preservation methods, and associated data they require. The Locator will search the database and return a list of tissue resources most likely to meet their requirements. When no match is obtained, the researcher is referred to the NCI Tissue Expediter [(301) 496-7147; e-mail: tissexp@mail.nih.gov]. The Tissue Expediter is a scientist who can help match researchers with appropriate resources or identify appropriate collaborators when those are necessary. NIDDK - Biologic Samples from Diabetic Study Foundation A portion (1/3) of all stored nonrenewable samples (plasma, serum, urine) from subjects enrolled in the Diabetes Control and Complications Trial (DCCT) is available for use by the scientific community to address questions for which these samples may be invaluable. Announcements for using this resource appear in the NIH Guide for Grants and Contracts periodically. Inquiries may be addressed to: Catherine C. Cowie, Ph.D., Director, Diabetes Epidemiology Program, NIDDK, 6707 Democracy Blvd., Room 691, MSC 5460, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892-5460. Phone: (301) 594-8804; fax: (301) 480-3503; e-mail: cowiec@extra.niddk.nih.gov. NIDDK - NIDDK Central Repositories (Diabetes Prevention Study) The NIDDK Central Repositories have selected biosamples from the DPT-1 (The Diabetes Prevention Type 1) study that are available to qualified investigators through an application process. These samples are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. Information about how to apply for these materials can be obtained from the NIDDK Central Repositories by contacting Ms. Helen Ray of RTI, 1-919-316-3418, or hmp@rti.org. Direct scientific-technical inquiry to the Project Officer of the NIDDK Central Repositories, Dr. Rebekah Rasooly, at phone: (301) 594-6007; e-mail: rr185i@nih.gov. Visit the Repositories Web site at http://www.niddkrepository.org. NICHD - Brain and Tissue Bank for Developmental Disorders The purpose of the Bank is to collect, preserve, and distribute human tissues to investigators interested in autism and developmental disorders; normal tissues may be available for other research purposes. Further information can be obtained at www.btbank.org. The contact persons are H. Ron Zielke or Sally Wisniewsky, University of Maryland (1-800-847-1539), and Carol Petito or Stephanie Lojko, University of Miami (1-800-592-7246). NICHD - Reproductive Tissue Sample Repository (RTSaR) The Reproductive Tissue Sample Repository (RTSaR) is a virtual repository with online tissue sample acquisition capabilities. The RTSaR provides investigators with real-time access to human and nonhuman primate tissue and fluid inventories from four tissue bank facilities that are supported through the Specialized Cooperative Centers Program in Reproduction Research. The tissue banks are located at the University of California, San Diego (human ovary bank), Stanford University (human endometrium and DNA bank), Johns Hopkins University (male reproductive tissues and fluids), and the Oregon National Primate Research Center (nonhuman primate tissues). The web site for the RTSaR is https://rtsar.nichd.nih.gov/rtsar/login. If you wish to access the RTSaR, you can request an id and password to access the system by contacting the network administrator at RTSaR@mail.nih.gov. Once you access the system, contact information for each bank is provided. Access is open to all investigators living in North America who are supported by research and research training grants from the NIH. One id and password will be provided to each principal investigator that can be utilized by any person working in the P.I.’s laboratory, or, in the case of institutional training grants (T32) and institutional career development award programs (K12), any person supported by the aforementioned awards. NCRR - Human Tissues and Organs Resource (HTOR) The Human Tissues and Organs Resource (HTOR) cooperative agreement supports a procurement network developed by the National Disease Research Interchange (NDRI), a not-for-profit organization. By collaborating with various medical centers, hospitals, pathology services, eye banks, tissue banks, and organ procurement organizations, HTOR provides a wide variety of human tissues and organs—both diseased and normal—to researchers for laboratory studies. Such samples include tissues from the central nervous system and brain, cardiovascular system, endocrine system, eyes, bone, and cartilage. For further information, consult the NDRI Web site (www.ndri.com) or contact Dr. John T. Lonsdale at NDRI, 8 Penn Center, 8th Floor, 1628 JFK Boulevard, Philadelphia, PA 19103. Phone: (800) 222-6374, ext. 271; fax: (215) 557-7154; e-mail: jlonsdale@ndriresource.org. The NDRI Web site is http://www.ndri.com. NCRR - Islet Cell Resource (ICR) With support from NCRR, 10 Islet Cell Resource (ICR) centers isolate, purify, and characterize human pancreatic islets for subsequent transplantation into patients with type I diabetes. The ICR centers procure whole pancreata and acquire relevant data about donors; improve islet isolation and purification techniques; distribute islets for use in approved clinical protocols; and perfect the methods of storage and shipping. In this way, the centers optimize the viability, function, and availability of islets and help clinical researchers capitalize on the recently reported successes in islet transplantation. Information on submitting requests for islet cells can be obtained from Mr. John Kaddis, ICR Coordinating Center Project Manager, City of Hope National Medical Center, 1500 E. Duarte Road, Duarte, California 91010. Phone (626) 359-8111, ext. 63377; fax: (626) 471-7106; e-mail: jkaddis@coh.org. The Coordinating Center hosts a Web site at http://icr.coh.org. NIA - SWAN Repository (longitudinal, multiethnic study of women at midlife including the menopausal transition) The SWAN Repository is a biologic specimen bank of the Study of Women’s Health Across the Nation (SWAN). The SWAN cohort was recruited in 1996/1997 and consists of 3302 African-American, Caucasian, Chinese, Hispanic, and Japanese women. The SWAN Repository contains more than 350,000 blood and urine specimens generated from the study participants’ annual visits (8 visits to date), at which time medical and health history, psychosocial measures, biological measures, and anthropometric data were and are being collected. In addition, a subset of the participants are providing urine samples, collected daily over the length of one menstrual cycle, each year. More than 900,000 of these samples are in the SWAN Repository and are available to researchers who wish to study the midlife and menopausal transition. Additionally, a DNA sample repository is also available and includes DNA as well as transformed B-lymphoblastoid cell lines from more than 1800 of the participants. To learn more about the SWAN Repository and how to apply to use SWAN Repository specimens, contact the Web site at http://www. swanrepository.com or Dr. MaryFran Sowers, University of Michigan, School of Public Health, Epidemiology Dept., (734) 936-3892; e-mail: mfsowers@umich.edu. HUMAN AND ANIMAL CELL AND BIOLOGIC REAGENT RESOURCES NIDDK - National Hormone and Peptide Program The National Hormone and Peptide Program (NHPP) offers peptide hormones and their antisera, tissues (rat hypothalami), and miscellaneous reagents to qualified investigators. These reagents are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. These materials can be obtained from Dr. A. F. Parlow of the Harbor-UCLA Medical Center, Research and Education Institute, Torrance, CA. A more complete description of resources within this program is provided in The Endocrine Society journals. Direct scientific-technical inquiry to NHPP Scientific Director, Dr. Al Parlow, at phone: (310) 222-3537; fax: (310) 222-3432; e-mail: parlow@humc.edu. Visit the NHPP Web site at http://www.humc.edu/hormones. NICHD - National Hormone and Pituitary Program (see NIDDK listing) Following is a list of reagents currently available through the resources of NICHD: Androgen receptor and peptide antigenRecombinant monkey (cynomolgus) and baboon luteinizing hormone and follicle-stimulating hormone and antisera. NIA - Aging Cell Bank To facilitate aging research on cells in culture, the NIA provides support for the Aging Cell Bank located at the Coriell Institute for Medical Research in Camden, NJ. The Aged Cell Bank provides fibroblast, lymphoblastoid, and differentiated cell lines from a wide range of human age-related conditions and other mammalian species, as well as DNA from a limited subset of cell lines. For further information, the Aged Cell Bank catalog can be accessed at http://locus.umdnj.edu/nia or contact Dr. Donald Coppock at 1-800-752-3805. NCRR - Various Cell Repositories NCRR maintains the following cell repository resources: National Cell Culture Center, National Stem Cell Resource, and the Yeast Genetic Stock Center. Further information regarding these resources may be obtained through the NCRR Web site at: www.ncrr.nih.gov/ncrrprog/cmpdir/BIOLOG.asp. ANIMAL RESOURCES NIA - Aging Rodent Resources NIA maintains both rat and mouse colonies for use by the scientific community. The animals available range in age from 1 to 36 months. A repository of fresh-frozen tissue from the NIA aged rodent colonies is stocked with tissue from mouse and rat strains, including caloric-restricted BALB/c mice. The NIA also maintains a colony of calorically restricted rodents of selected genotypes, which are available to the scientific community. For further information, please refer to the Aged Rodent information handbook at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentColoniesHandbook/ or contact the Office of Biological Resources and Resource Development order desk. Phone: (301) 496-0181; fax: (301) 402-5597; e-mail: rodents@nia.nih.gov. NIA - Aged Rodent Tissue Bank The rodent tissue bank contains flash-frozen tissues from rodents in the NIA aged rodent colonies. Tissue is collected from rodents at 4 or 5 age points throughout the lifespan. Tissue arrays are also available. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentTissueBankHandbook/. NCRR - Mutant Mouse Regional Resource Centers (MMRRC) The Mutant Mouse Regional Resource Center (MMRRC) Program consists of centers that collectively operate as a one-stop shop to serve the biomedical research community. Investigators who have created select mutant mouse models may donate their models to an MMRRC for broad dissemination to other investigators who request them for noncommercial research investigations related to human health, disease, and treatments. The NCRR Division of Comparative Medicine (DCM) supports the MMRRCs, which are electronically linked through the MMRRC Informatics Coordinating Center (ICC) to function as one facility. The ICC, located at The Jackson Laboratory in Bar Harbor, ME, provides database and other informatics support to the MMRRC to give the research community a single entry point to the program. Further information can be obtained from the Web site at http://www.mmrrc.org, or from Franziska Grieder, D.V.M., Ph.D., Division of Comparative Medicine, NCRR. Phone (301) 435-0744; fax: (301) 480-3819; e-mail: griederf@ncrr.nih.gov. NCRR - Induced Mutant Mouse Resource (IMR) The Induced Mutant Mouse Resource (IMR) at The Jackson Laboratory provides researchers with genetically engineered mice (transgenic, targeted mutant, retroviral insertional mutant, and chemically induced mutant mice). The function of the IMR is to select, import, cryopreserve, maintain, and distribute these important strains of mice to the research community. To improve their value for research, the IMR also undertakes genetic development of stocks, such as transferring mutant genes or transgenes to defined genetic backgrounds and combining transgenes and/or targeted mutations to create new mouse models for research. Over 800 mutant stocks have been accepted by the IMR. Current holdings include models for research on cancer, immunological and inflammatory diseases, neurological diseases and behavioral disorders, cardiovascular diseases, developmental disorders, metabolic and other diseases, reporter (e.g. GFP) and recombinase (e.g. cre/loxP) strains. About 8 strains a month are being added to the IMR holdings. A list of all strains may be obtained from the IMR Web site: www.jax.org/resources/documents/imr/. Online submission forms are also available on that site. All mice can be ordered by calling The Jackson Laboratory’s Customer Service Department at 1-800-422-MICE or (207) 288-5845 or by faxing (207) 288-6150. NIDDK - Mouse Metabolic Phenotyping Centers The mission of the Mouse Metabolic Phenotyping Centers is to provide the scientific community with standardized, high-quality metabolic and physiologic phenotyping services for mouse models of diabetes, diabetic complications, obesity, and related disorders. Researchers can ship mice to one of the four Centers (University of Cincinnati, University of Texas Southwestern Medical Center, Vanderbilt University, and Yale University) and obtain on a fee-for-service basis a range of complex exams used to characterize mouse metabolism, blood composition, energy balance, eating and exercise, organ function and morphology, physiology, and histology. Many tests are done in living animals and are designed to elucidate the subtle hallmarks of metabolic disease. Information, including a complete list of available tests, can be found at www.mmpc.org, or contact Dr. Maren R. Laughlin, NIDDK, at (301) 594-8802; e-mail: Maren.Laughlin@nih.gov; or Dr. Kristin Abraham, NIDDK, at (301) 451-8048; e-mail: abrahamk@extra.niddk.nih.gov. NCRR - National Primate Research Centers (NPRCs) National Primate Research Centers (NPRCs) are a network of eight highly specialized facilities for nonhuman primates (NHP) research. Funded by grants through NCRR’s Division of Comparative Medicine (DCM), each center, staffed with experienced research and support staff, provides the appropriate research environment to foster the development of NHP models of human health and disease for biomedical investigations. The NPRCs are affiliated with academic institutions and are accessible to eligible biomedical and behavioral investigators supported by research project grants from the National Institutes of Health and other sources. Further information may be obtained from the notice, Procedures for Accessing Regional Primate Research Centers, published in the NIH Guide for Grants and Contracts at http://grants2.nih.gov/grants/guide/notice-files/not97-014.html, or from John Harding, Ph.D., National Primate Research Centers and AIDS Animal Models Program, Division of Comparative Medicine, NCRR. Phone: (301) 435-0744; fax: (301) 480-3819; e-mail: hardingj@mail.nih.gov. NIA - Nonhuman Primates, Aging Set-Aside Colony NIA maintains approximately 200 nonhuman primates (M. mulatta) at four National Primate Research Centers (see above) for conducting research on aging. These animals range in age from 18 to 35 years. While these animals are predominantly reserved for non-invasive research, exceptions can be made to this policy. For further information, please contact Dr. Nancy Nadon, Office of Biological Resources and Resource Development, NIA. Phone: (301) 402-7744; fax: (301) 402-0010; e-mail: nadonn@nia.nih.gov. NIA - Nonhuman Primate (NHP) Tissue Bank and Aging Database The NIA developed two new resources to facilitate research in the NHP model. The NHP tissue bank contains fresh-frozen and fixed tissue donated by primate centers around the country. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/NHPTissueBankHandbook.htm. The Primate Aging Database provides an internet accessible database with data from thousands of primates around the country. It can be used to investigate the effect of age on a variety of parameters, predominantly blood chemistry and husbandry measurements. The site is password protected. The URL is http://ipad.primate.wisc.edu. NIA - Obesity, Diabetes and Aging Animal Resource (USF-ODARC) The NIA supports a colony of aged rhesus macaques, many of which are obese and/or diabetic. This is a long-term colony of monkeys housed at the University of South Florida’s Obesity, Diabetes and Aging Research Center. They have been extensively and longitudinally characterized for general health variables, blood chemistry, food intake, and body weight. Diabetic monkeys are tested daily for urine glucose and ketone levels, and prediabetic monkeys are tested weekly. Data for some of the monkeys extend as far back as 15 years. This unique resource is available for collaborative studies. ODARC has a significant amount of stored tissue collected at necropsy and stored blood/plasma collected longitudinally. Serial blood collection or tissue collection at necropsy can also be performed prospectively. Testing and imaging can also be performed on the monkeys. Inquiries regarding collaborative studies using the ODARC colony should be directed to: Barbara C. Hansen, Ph.D., Director, Obesity, Diabetes and Aging Research Center, University of South Florida, All Children’s Hospital, 801 6th Street South #9340, St. Petersburg, FL 33701. Phone: (727) 767-6993; fax: (727) 767-7443; e-mail: bchansen@aol.com. NCRR - Various Animal Resources NCRR maintains the following animal resources: Animal Models and Genetic Stocks, Chimpanzee Biomedical Research Program, NIH Animal Genetic Resource, and the Specific Pathogen Free Macaque Breeding and Research Program. Further information regarding these and other resources may be obtained through the NCRR Web site at www.ncrr.nih.gov/comparative_med.asp. IN SILICO RESOURCES NIDDK, NHLBI, and NIEHS - Nuclear Receptor Signaling Atlas The Nuclear Receptor Signaling Atlas (NURSA) has created an in silico resource comprised of curated information about Nuclear Receptors, Coregulators, Ligands, and Downstream Targets. NURSA is sponsored by NIH and provides online access through a public webportal at www.NURSA.org. Ease of navigation through a series of molecule pages allows users to make queries about Nuclear Receptors, Coactivators and Corepressors. Additional information about nuclear receptor ligands is provided, as well as primary datasets relating to expression profiling of nuclear receptors, coregulators and downstream targets. The molecule pages are hyperlinked to data contained in external databases, including NCBI, KEGG, UniProt, and others, allowing for detailed data mining. In partnership with The Endocrine Society, NURSA and Molecular Endocrinology (http://mend.endojournals.org/) have reciprocal links designed to enhance publications in Molecular Endocrinology and the information available through the NURSA molecule pages. Links to additional relevant literature citations are from PubMed at the National Library of Medicine. MISCELLANEOUS RESOURCES NCRR - National Gene Vector Laboratories (NGVLs) The National Gene Vector Laboratories (NGVLs), with core funding from NCRR, serve as a resource for researchers to obtain adequate quantities of clinical-grade vectors for human gene transfer protocols. The vector types include retrovirus, lentivirus, adenovirus, adeno-associated virus, herpes-virus, and DNA plasmids. The NGVLs consist of three vector production centers at: Baylor College of Medicine; City of Hope National Medical Center and Beckman Research Institute; and Indiana University, which also serves as the Coordinating Center for all the laboratories. Two additional laboratories conduct toxicology studies for NGVL-approved investigators. These laboratories are located at the Southern Research Institute and the University of Florida. Additional information about the process for requesting vector production and/or pharmacology/toxicology support should be directed to Ms. Lorraine Matheson, NGVL Project Coordinator, Indiana University School of Medicine. Phone: (317) 274-4519; fax: (317) 278-4518; e-mail: lrubin@iupui.edu. The NGVL Coordinating Center at Indiana University also hosts a Web site at http://www.ngvl.org. NCRR - General Clinical Research Centers (GCRCs) The General Clinical Research Centers (GCRCs) are a national network of 82 centers that provide optimal settings for medical investigators to conduct safe, controlled, state-of-the-art in-patient and out-patient studies of both children and adults. GCRCs also provide infrastructure and resources that support several career development opportunities. Investigators who have research project funding from the National Institutes of Health (NIH) and other peer-reviewed sources may apply to use GCRCs. Because the GCRCs support a full spectrum of patient-oriented scientific inquiry, researchers who use these centers can benefit from collaborative, multidisciplinary research opportunities. To request access to a GCRC facility, eligible investigators should initially contact a GCRC program director, listed in the National Center for Research Resources (NCRR) Clinical Research Resources Directory (www.ncrr.nih.gov/ncrrprog/clindir/crdirectory.asp). Further information can be obtained from Anthony R. Hayward, M.D., Director, Division for Clinical Research Resources, National Center for Research Resources at NIH. Phone: (301) 435-0790; e-mail: haywarda@ncrr.nih.gov.

While exploring the virtual world Second Life one day, I received a group message across the in-world communication system – “there’s a griefer on the beach. Stay away from the beach till we catch him.” There was no need to explain; everyone receiving the message knew what a griefer was and had a general idea of the kinds of things that could be happening. We’d all seen griefers at work before – someone monopolising the chat channel so no one else can communicate, people being “caged” at random, or even weapons fire causing so much “overhead” that all activity in the area slows to a crawl. These kinds of attacks are not limited to virtual worlds. Most people have experienced griefing in their everyday lives, which might best be defined as having fun at someone else’s expense. More commonly seen examples of this in the real world include teasing, bullying, and harassment; playground bullies have long made other children’s free time miserable. More destructive griefing includes arson and theft. Griefing activities happen in all kinds of games and virtual worlds. Griefers who laugh at new users and “yell” (so that all players can hear) that they stink, have followed new users of Disney’s tween-popular ToonTown. Griefers pose as friendly, helpful players who offer to show new users a path through difficult parts of a game, but then who abandon the new user in a spot where he or she does not have the skills to proceed. In World of Warcraft, a popular massively multiplayer online role playing game (MMORPG) created by Blizzard with more than seven million registered, if not active, users, griefers engage in what is known as corpse camping; they sit by a corpse, killing it over and over every time the player tries to get back into the game. The griefer gets a small number of experience points; the player being killed gets aggravated and has to wait out the griefing to play the game again (Warner & Raiter). Griefing in World of Warcraft was featured in an award nominated episode of the television program South Park, in which one character killed every other player he met. This paper considers different types of griefing, both in online games and virtual worlds, and then looks at the actions other players, those being griefed, take against griefers. A variety of examples from Second Life are considered because of the open-structure of the world and its developing nature. Definitions and Types Griefing in online environments such as video games and virtual worlds has been defined as “purposefully engaging in activities to disrupt the gaming experience of other players” (Mulligan & Patrovsky 250). The “purposeful” part of the definition means that accidental bumping and pushing, behaviours often exhibited by new users, are not griefing (Warner & Raiter). Rossingol defines a griefer as, “a player of malign intentions. They will hurt, humiliate and dishevel the average gamer through bending and breaking the rules of online games. ...They want glory, gain or just to partake in a malignant joy at the misfortune of others.” Davis, who maintains a gaming blog, describes Second Life as being populated by “those who build things and those who like to tear them down,” with the latter being the griefers who may be drawn to the unstructured anything-goes nature of the virtual world (qtd. in Girard). Definitions of griefing differ based on context. For instance, griefing has been examined in a variety of multi-player online games. These games often feature missions where players have to kill other players (PvP), behaviour that in other contexts such as virtual worlds would be considered griefing. Putting a monster on the trail of a player considered rude or unskilled might be a way to teach a lesson, but also an example of griefing (Taylor). Foo and Koivisto define griefing in MMORPGs as “play styles that disrupt another player’s gaming experience, usually with specific intention. When the act is not specifically intended to disrupt and yet the actor is the sole beneficiary, it is greed play, a subtle form of grief play” (11). Greed play usually involves actions that disrupt the game play of others but without technically breaking any game rules. A different way of looking at griefing is that it is a sign that the player understands the game or virtual world deeply enough to take advantage of ambiguities in the rules by changing the game to something new (Koster). Many games have a follow option; griefers pick a victim, stand near them, get as naked as possible, and then just follow them around without talking or explaining their actions (Walker). Another example is the memorial service in World of Warcraft for a player who died in real life. The service was interrupted by an attack from another clan; everyone at the memorial service was killed. It is not clear cut who the griefers actually were in this case – the mourners who chose to have their peaceful service in an area marked for player combat or the attackers following the rules for that area and working to earn points and progress in the game. In the case of the mourners, they were changing the rules of the game to suit them, to create something unique – a shared space to mourn a common friend. But they were definitely not playing by the rules. The attackers, considered griefers by many both in and outside of the game, did nothing that broke any rules of the game, though perhaps they broke rules of common decency (“World”); what they did does not fit into the definition of griefing, as much as do the actions of the mourners (Kotaku). Reshaping the game can be done to embed a new, sometimes political, message into the game. A group named Velvet Strike formed to protest US military action. They went into Counter Strike to bring a “message of peace, love and happiness to online shooters by any means necessary” (King). They placed spray painted graphics containing anti-war messages into the game; when confronted with people from other teams the Velvet Strike members refused to shoot (King). The group website contains “recipes” for non-violent game play. One “recipe” involved the Velvet Strike member hiding at the beginning of a mission and not moving for the rest of the game. The other players would shoot each other and then be forced to spend the rest of the game looking for the last survivor in order to get credit for the win. Similar behaviour has been tried inside the game America’s Army. Beginning March, 2006, deLappe, an artist who opposes the U.S. government’s involvement in Iraq, engaged in griefing behaviour by filling (spamming) the in-game text channel with the names of the people killed in the war; no one else can communicate on that channel. Even his character name, dead-in-Iraq, is an anti-war protest (deLappe). “I do not participate in the proscribed mayhem. Rather, I stand in position and type until I am killed. After death, I hover over my dead avatar’s body and continue to type. Upon being re-incarnated in the next round, I continue the cycle” (deLappe n.p.). What about these games and virtual worlds might lead people to even consider griefing? For one thing, they seem anonymous, which can lead to irresponsible behaviour. Players use fake names. Characters on the screen do not seem real. Another reason may be that rules can be broken in videogames and virtual worlds with few consequences, and in fact the premise of the game often seems to encourage such rule breaking. The rules are not always clearly laid out. Each game or world has a Terms of Service agreement that set out basic acceptable behaviour. Second Life defines griefing in terms of the Terms of Service that all users agree to when opening accounts. Abuse is when someone consciously and with malicious intent violates those terms. On top of that limited set of guidelines, each landowner in a virtual world such as Second Life can also set rules for their own property, from dress code, to use of weapons, to allowable conversation topics. To better understand griefing, it is necessary to consider the motivations of the people involved. Early work on categorising player types was completed by Bartle, who studied users of virtual worlds, specifically MUDs, and identified four player types: killers, achievers, socialisers, and explorers. Killers and achievers seem most relevant in a discussion about griefing. Killers enjoy using other players to get ahead. They want to do things to other people (not for or with others), and they get the most pleasure if they can act without the consent of the other player. Knowing about a game or a virtual world gives no power unless that knowledge can be used to gain some advantage over others and to enhance your standing in the game. Achievers want power and dominance in a game so they can do things to the game and master it. Griefing could help them feel a sense of power if they got people to do their will to stop the griefing behavior. Yee studied the motivations of people who play MMORPGs. He found that people who engage in griefing actually scored high in being motivated to play by both achieving and competition (“Facets”). Griefers often want attention. They may want to show off their scripting skills in the hope of earning respect among other coders and possibly be hired to program for others. But many players are motivated by a desire to compete and to win; these categories do not seem to be adequate for understanding the different types of griefing (Yee, “Faces of Grief”). The research on griefing in games has also suggested ways to categorise griefers in virtual worlds. Suler divides griefers into two types (qtd. in Becker). The first is those who grief in order to make trouble for authority figures, including the people who create the worlds. A few of the more spectacular griefing incidents seem designed to cause trouble for Linden Lab, the creators of Second Life. Groups attacked the servers that run Second Life, known as the grid, in October of 2005; this became known as the “gray goo attack” (Second Life; Wallace). Servers were flooded with objects and Second Life had to be taken off line to be restored from backups. More organised groups, such as the W-hats, the SL Liberation Army, and Patriotic Nigas engage in more large scale and public griefing. Some groups hope to draw attention to the group’s goals. The SL Liberation Army wants Linden Lab to open up the governance of the virtual world so that users can vote on changes and policies being implemented and limit corporate movement into Second Life (MarketingVox). Patriotic Nigas, with about 35 active members, want to slow the entry of corporations into Second Life (Cabron, “Who are Second Life’s”). One often discussed griefer attack in Second Life included a flood of pink flying penises directed against land owner and the first person to have made a profit of more than one million United States dollars in a virtual world, Anshe Chung, during a well-publicised and attended interview in world with technology news outlet CNET (Walsh, “Second Life Millionaire” ). The second type proposed by Suler is the griefer who wants to hurt and victimise others (qtd. in Becker). Individual players often go naked into PG-rated areas to cause trouble. Weapons are used in areas where weapons are banned. Second Life publishes a police blotter, which lists examples of minor griefing and assigned punishment, including incidents of disturbing the peace and violating community standards for which warnings and short bans have been issued. These are the actions of individuals for the most part, as were the people who exploited security holes to enter the property uninvited during the grand opening of Endemol’s Big Brother island in Second Life; guests to the opening were firebombed and caged. One of the griefers explained her involvement: Well I’m from The Netherlands, and as you might know the tv concept of big brother was invented here, and it was in all the newspapers in Holland. So I thought It would be this huge event with lots of media. Then I kinda got the idea ‘hey I could ruin this and it might make the newspaper or tv. So that’s what set me off, lol. (qtd. in Sklar) Some groups do grief just to annoy. The Patriotic Nigas claim to have attacked the John Edwards headquarters inside SL wearing Bush ‘08 buttons (Cabron, “John Edwards Attackers”), but it was not a political attack. The group’s founder, Mudkips Acronym (the name of his avatar in SL) said, “I’m currently rooting for Obama, but that doesn’t mean we won’t raid him or anything. We’ll hit anyone if it’s funny, and if the guy I want to be president in 2008’s campaign provides the lulz, we’ll certainly not cross him off our list” (qtd. in Cabron, “John Edwards Attackers”). If they disrupt a high profile event or site, the attack will be covered by media that can amplify the thrill of the attack, enhance their reputation among other griefers, and add to their enjoyment of the griefing. Part of the definition of griefing is that the griefer enjoys causing other players pain and disrupting their game. One resident posted on the SL blog, “Griefers, for the most part, have no other agenda other than the thrill of sneaking one past and causing a big noise. Until a spokesperson comes forward with a manifesto, we can safely assume that this is the work of the “Jackass” generation, out to disrupt things to show that they can“ (Scarborough). Usually to have fun, griefers go after individuals, rather than the owners and administrators of the virtual world and so fit into Suler’s second type of griefing. These griefers enjoy seeing others get angry and frustrated. As one griefer said: Understanding the griefer mindset begins with this: We don’t take the game seriously at all. It continues with this: It’s fun because you react. Lastly: We do it because we’re jerks and like to laugh at you. I am the fly that kamikazes into your soup. I am the reason you can’t have nice things … . If I make you cry, you’ve made my day. (Drake) They have fun by making the other players mad. “Causing grief is the name of his game. His objective is simple: Make life hell for anyone unlucky enough to be playing with him. He’s a griefer. A griefer is a player bent on purposely frustrating others during a multiplayer game” (G4). “I’m a griefer. It’s what I do,” the griefer says. “And, man, people get so pissed off. It’s great” (G4). Taking Action against Griefers Understanding griefing from the griefer point of view leads us to examine the actions of those being griefed. Suler suggests several pairs of opposing actions that can be taken against griefers, based on his experience in an early social environment called Palace. Many of the steps still being used fit into these types. He first describes preventative versus remedial action. Preventative steps include design features to minimise griefing. The Second Life interface includes the ability to build 3D models and to create software; it also includes a menu for land owners to block those features at will, a design feature that helps prevent much griefing. Remedial actions are those taken by the administrators to deal with the effects of griefing; Linden Lab administrators can shut down whole islands to keep griefer activities from spreading to nearby islands. The second pair is interpersonal versus technical; interpersonal steps involve talking to the griefers to get them to stop ruining the game for others, while technical steps prevent griefers from re-entering the world. The elven community in Second Life strongly supports interpersonal steps; they have a category of members in their community known as guardians who receive special training in how to talk to people bent on destroying the peacefulness of the community or disturbing an event. The creators of Camp Darfur on Better World island also created a force of supporters to fend off griefer attacks after the island was destroyed twice in a week in 2006 (Kenzo). Linden Lab also makes use of technical methods; they cancel accounts so known griefers can not reenter. There were even reports that they had created a prison island where griefers whose antics were not bad enough to be totally banned would be sent via a one-way teleporter (Walsh, “Hidden Virtual World Prison”). Some users of Second Life favour technical steps; they believe that new users should be held a fixed amount of time on the Orientation island which would stop banned users from coming back into the world immediately. The third is to create tools for average users or super users (administrators); both involve software features, some of which are available to all users to help them make the game good for them while others are available only to people with administrator privileges. Average users who own land have a variety of tools available to limit griefing behaviour on their own property. In Second Life, the land owner is often blamed because he or she did not use the tools provided to landowners by Linden Lab; they can ban individual users, remove users from the land, mute their conversation, return items left on the property, and prevent people from building or running scripts. As one landowner said, “With the newbies coming in there, I’ve seen their properties just littered with crap because they don’t know protective measures you need to take as far as understanding land control and access rights” (qtd. in Girard). Super users, those who work for Linden Lab, can remove a player from the game for a various lengths of time based on their behaviour patterns. Responses to griefers can also be examined as either individual or joint actions. Individual actions include those that land owners can take against individual griefers. Individual users, regardless of account type, can file abuse reports against other individuals; Linden Lab investigates these reports and takes appropriate action. Quick and consistent reporting of all griefing, no matter how small, is advocated by most game companies and user groups as fairly successful. Strangely, some types of joint actions have been not so successful. Landowners have tried to form the Second Life Anti-Griefing Guild, but it folded because of lack of involvement. Groups providing security services have formed; many event organisers use this kind of service. (Hoffman). More successful efforts have included the creation of software, such as SLBanLink.com, Karma, and TrustNet that read lists of banned users into the banned list on all participating property. A last category of actions to be taken against griefers, and a category used by most residents of virtual worlds, is to leave them alone—to ignore them, to tolerate their actions. The thinking is that, as with many bullies in real life, griefers want attention; when deprived of that, they will move on to find other amusements. Yelling and screaming at griefers just reinforces their bad behaviour. Users simply teleport to other locations or log off. They warn others of the griefing behaviour using the various in-world communication tools so they too can stay away from the griefers. Most of the actions described above are not useful against griefers for whom a bad reputation is part of their credibility in the griefer community. The users of Second Life who staged the Gray Goo denial of service attack in October, 2005 fit into that category. They did nothing to hide the fact that they wanted to cause massive trouble; they named the self-replicating object that they created Grief Spawn and discussed ways to bring down the world on griefer forums (Wallace) Conclusion The most effective griefing usually involves an individual or small group who are only looking to have fun at someone else’s expense. It’s a small goal, and as long as there are any other users, it is easy to obtain the desired effect. In fact, as word spreads of the griefing and users feel compelled to change their behaviour to stave off future griefer attacks, the griefers have fun and achieve their goal. The key point here is that everyone has the same goal – have fun. Unfortunately, for one group – the griefers – achieving their goal precludes other users from reaching theirs. Political griefers are less successful in achieving their goals. Political creative play as griefing, like other kinds of griefing, is not particularly effective, which is another aspect of griefing as error. Other players react with frustration and violence to the actions of griefers such as deLappe and Velvet-Strike. If griefing activity makes people upset, they are less open to considering the political or economic motives of the griefers. Some complaints are relatively mild; “I’m all for creative protest and what not, but this is stupid. It’s not meaningful art or speaking out or anything of the type, its just annoying people who are never going to change their minds about how awesome they think war is” (Borkingchikapa). Others are more negative: “Somebody really needs to go find where that asshole lives and beat the shit out of him. Yeah, it’s a free country and he can legally pull this crap, but that same freedom extends to some patriot kicking the living shit out of him” (Reynolds). In this type of griefing no one’s goals for using the game are satisfied. The regular users can not have fun, but neither do they seem to be open to or accepting of the political griefer’s message. This pattern of success and failure may explain why there are so many examples of griefing to disrupt rather then the politically motivated kind. It may also suggest why efforts to curb griefing have been so ineffective in the past. Griefers who seek to disrupt for fun would see it as a personal triumph if others organised against them. Even if they found themselves banned from one area, they could quickly move somewhere else to have their fun since whom or where they harass does not really matter. Perhaps not all griefing is in error, rather, only those griefing activities motivated by any other goal than have fun. People invest their time and energy in creating their characters and developing skills. The behaviour of people in these virtual environments has a definite bearing on the real world. And perhaps that explains why people in these virtual worlds react so strongly to the behaviour. So, remember, stay off the beach until they catch the griefers, and if you want to make up the game as you go along, be ready for the other players to point at you and say “Bad, Bad Avatar.” References Bartle, Richard. “Players Who Suit MUDs.” Journal of MUD Research 1.1 (June 1996). 10 Sep. 2007 http://www.mud.co.uk/richard/hcds.htm>. Becker, David. Inflicting Pain on “Griefers.” 13 Dec. 2004. 10 Oct. 2007 http://www.news.com/Inflicting-pain-on-griefers/2100-1043_3-5488403.html>. Borkingchikapa. Playing America’s Army. 30 May 2006. 10 Aug. 2007 http://www.metafilter.com/51938/playing-Americas-Army>. Cabron, Lou. John Edwards Attackers Unmasked. 5 Mar. 2007. 29 Apr. 2007 http://www.10zenmonkeys.com/2007/03/05/john-edwards-virtual-attackers-unmasked/>. Cabron, Lou. 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N.D. 10 Aug. 2007 http://www.opensorcery.net/velvet-strike/nonflame.html>. Walker, John. “How to Be a Complete Bastard.” PC Gamer 13 Mar. 2007. 10 Aug. 2007 http://www.computerandvideogames.com/article.php?id=159883&site=pcg>. Wallace, Mark. “The Day the Grid Disappeared.” Escapist Magazine 15 Nov. 2005: 11. 20 June 2007 http://www.escapistmagazine.com/issue/19/11>. Walsh, Tony. Hidden Virtual-World Prison Revealed. 3 Jan. 2006. 10 Oct. 2007 http://www.secretlair.com/index.php?/clickableculture/entry/hidden_virtual_world_prison_revealed/>. Walsh, Tony. Second Life Millionaire Interview Penis-Bombed. 20 Dec. 2006. 10 Oct. 2007 http://www.secretlair.com/index.php?/clickableculture/entry/second_life_millionaire_interview_penis_bombed/>. Warner, Dorothy, and Mike Raiter. _Social Context in Massively-Multiplayer Online Games. _2005. 20 Aug. 2007 http://www.i-r-i-e.net/inhalt/004/Warner-Raiter.pdf>. “World of Warcraft: Funeral Ambush.” 2006. YouTube. 15 Aug. 2007 http://www.youtube.com/watch?v=31MVOE2ak5w>. Yee, Nicholas. Facets: 5 Motivational Factors for Why People Play MMORPG’s. 2002. 10 Oct. 2007 http://www.nickyee.com/facets/home.html>. Yee, Nicholas. Faces of Grief. 2005. June 2007 http://www.nickyee.com/daedalus/archives/000893.php?page=1>. Citation reference for this article MLA Style Gregson, Kimberly. "Bad Avatar!: Griefing in Virtual Worlds." M/C Journal 10.5 (2007). echo date('d M. Y'); ?> <http://journal.media-culture.org.au/0710/06-gregson.php>. APA Style Gregson, K. (Oct. 2007) "Bad Avatar!: Griefing in Virtual Worlds," M/C Journal, 10(5). Retrieved echo date('d M. Y'); ?> from <http://journal.media-culture.org.au/0710/06-gregson.php>.

This paper provides embedded, reflective practice-based insight arising from my experience collaborating to produce online and print-on-demand editions of a magazine showcasing the photography of members of haphazart! Contemporary Abstracts group (hereafter referred to as haphazart!). The group’s online visual, textual and activity-based practices via the photo sharing social networking site Flickr are portrayed as achieving cohesive visual identity. Stylistic analysis of pictures in support of this claim is not attempted. Rather negotiation, that Elliot has previously described in M/C Journal as innate in collaboration, is identified as the unifying factor. However, the collaborators’ adherence to Flickr’s communication platform proves problematic in the editorial context. Some technical incoherence with possible broader cultural implications is encountered during the process of repurposing images from screen to print. A Scan of Relevant Literature The photographic gaze perceives and captures objects which seem to ‘carry within them ready-made’ a work of art. But the reminiscences of the gaze are only made possible by knowing and associating with groups that define a tradition. The list of valorised subjects is not actually defined with reference to a culture, but rather by familiarity with a limited group. (Chamboredon 144) As part of the array of socio-cultural practices afforded by Web 2.0 interoperability, sites of produsage (Bruns) are foci for studies originating in many disciplines. Flickr provides a rich source of data that researchers interested in the interface between the technological and the social find useful to analyse. Access to the Flickr application programming interface enables quantitative researchers to observe a variety of means by which information is propagated, disseminated and shared. Some findings from this kind of research confirm the intuitive. For example, Negoecsu et al. find that “a large percentage of users engage in sharing with groups and that they do so significantly” ("Analyzing Flickr Groups" 425). They suggest that Flickr’s Groups feature appears to “naturally bring together two key aspects of social media: content and relations.” They also find evidence for what they call hyper-groups, which are “communities consisting of groups of Flickr groups” ("Flickr Hypergroups" 813). Two separate findings from another research team appear to contradict each other. On one hand, describing what they call “social cascades,” Cha et al. claim that “content in the form of ideas, products, and messages spreads across social networks like a virus” ("Characterising Social Cascades"). Yet in 2009 they claim that homocity and reciprocity ensure that “popularity of pictures is localised” ("Measurement-Driven Analysis"). Mislove et al. reflect that the affordances of Flickr influence the growth patterns they observe. There is optimism shared by some empiricists that through collation and analysis of Flickr tag data, the matching of perceptual structures of images and image annotation techniques will yield ontology-based taxonomy useful in automatic image annotation and ultimately, the Semantic Web endeavour (Kennedy et al.; Su et al.; Xu et al.). Qualitative researchers using ethnographic interview techniques also find Flickr a valuable resource. In concluding that the photo sharing hobby is for many a “serious leisure” activity, Cox et al. propose that “Flickr is not just a neutral information system but also value laden and has a role within a wider cultural order.” They also suggest that “there is genuinely greater scope for individual creativity, releasing the individual to explore their own identity in a way not possible with a camera club.” Davies claims that “online spaces provide an arena where collaboration over meanings can be transformative, impacting on how individuals locate themselves within local and global contexts” (550). She says that through shared ways of describing and commenting on images, Flickrites develop a common criticality in their endeavour to understand images, each other and their world (554).From a psychologist’s perspective, Suler observes that “interpersonal relationships rarely form and develop by images alone” ("Image, Word, Action" 559). He says that Flickr participants communicate in three dimensions: textual (which he calls “verbal”), visual, and via the interpersonal actions that the site affords, such as Favourites. This latter observation can surely be supplemented by including the various games that groups configure within the constraints of the discussion forums. These often include submissions to a theme and voting to select a winning image. Suler describes the place in Flickr where one finds identity as one’s “cyberpsychological niche” (556). However, many participants subscribe to multiple groups—45.6% of Flickrites who share images share them with more than 20 groups (Negoescu et al., "Analyzing Flickr Groups" 420). Is this a reflection of the existence of the hyper-groups they describe (2009) or, of the ranging that people do in search of a niche? It is also probable that some people explore more than a singular identity or visual style. Harrison and Bartell suggest that there are more interesting questions than why users create media products or what motivates them to do so: the more interesting questions center on understanding what users will choose to do ultimately with [Web2.0] capabilities [...] in what terms to define the success of their efforts, and what impact the opportunity for individual and collaborative expression will have on the evolution of communicative forms and character. (167) This paper addresseses such questions. It arises from a participatory observational context which differs from that of the research described above. It is intended that a different perspective about online group-based participation within the Flickr social networking matrix will avail. However, it will be seen that the themes cited in this introductory review prove pertinent. Context As a university teacher of a range of subjects in the digital media field, from contemporary photomedia to social media to collaborative multimedia practice, it is entirely appropriate that I embed myself in projects that engage, challenge and provide me with relevant first-hand experience. As an academic I also undertake and publish research. As a practicing new media artist I exhibit publically on a regular basis and consider myself semi-professional with respect to this activity. While there are common elements to both approaches to research, this paper is written more from the point of view of ‘reflective practice’ (Holmes, "Reconciling Experimentum") rather than ‘embedded ethnography’ (Pink). It is necessarily and unapologetically reflexive. Abstract Photography Hyper-Group A search of all Flickr groups using the query “abstract” is currently likely to return around 14,700 results. However, only in around thirty of them does the group name, its stated rules and, the stream of images that flow through the pool arguably reflect a sense of collective concept and aesthetic that is coherently abstract. This loose complex of groups comprises a hyper-group. Members of these groups often have co-memberships, reciprocal contacts, and regularly post images to a range of groups and comment on others’ posts to be found throughout. Given that one of Flickr’s largest groups, Black and White, currently has around 131,150 members and hosts 2,093,241 items in its pool, these abstract special interest groups are relatively small. The largest, Abstract Photos, has 11,338 members and hosts 89,306 items in its pool. The group that is the focus of this paper, haphazart!, currently has 2,536 members who have submitted 53,309 items. The group pool is more like a constantly flowing river because the most recently added images are foremost. Older images become buried in an archive of pages which cannot be reverse accessed at a rate greater than the seven pages linked from a current view. A member’s presence is most immediate through images posted to a pool. This structural feature of Flickr promotes a desire for currency; a need to post regularly to maintain presence. Negotiating Coherence to the Abstract The self-managing social dynamics in groups has, as Suler proposes to be the case for individuals, three dimensions: visual, textual and action. A group integrates the diverse elements, relationships and values which cumulatively constitute its identity with contributions from members in these dimensions. First impressions of that identity are usually derived from the group home page which consists of principal features: the group name, a selection of twelve most recent posts to the pool, some kind of description, a selection of six of the most recent discussion topics, and a list of rules (if any). In some of these groups, what is considered to constitute an abstract photographic image is described on the group home page. In some it is left to be contested and becomes the topic of ongoing forum debates. In others the specific issue is not discussed—the images are left to speak for themselves. Administrators of some groups require that images are vetted for acceptance. In haphazart! particular administrators dutifully delete from the pool on a regular basis any images that they deem not to comply with the group ethic. Whether reasons are given or not is left to the individual prosecutor. Mostly offending images just disappear from the group pool without trace. These are some of the ways that the coherence of a group’s visual identity is established and maintained. Two groups out of the abstract photography hyper-group are noteworthy in that their discussion forums are particularly active. A discussion is just the start of a new thread and may have any number of posts under it. At time of writing Abstract Photos has 195 discussions and haphazart! — the most talkative by this measure—has 333. Haphazart! invites submissions of images to regularly changing themes. There is always lively and idiosyncratic banter in the forum over the selection of a theme. To be submitted an image needs to be identified by a specific theme tag as announced on the group home page. The tag can be added by the photographer themselves or by anyone else who deems the image appropriate to the theme. An exhibition process ensues. Participant curators search all Flickr items according to the theme tag and select from the outcome images they deem to most appropriately and abstractly address the theme. Copies of the images together with comments by the curators are posted to a dedicated discussion board. Other members may also provide responses. This activity forms an ongoing record that may serve as a public indicator of the aesthetic that underlies the group’s identity. In Abstract Photos there is an ongoing discussion forum where one can submit an image and request that the moderators rule as to whether or not the image is ‘abstract’. The same group has ongoing discussions labelled “Hall of Appropriate” where worthy images are reposted and celebrated and, “Hall of Inappropriate” where images posted to the group pool have been removed and relegated because abstraction has been “so far stretched from its definition that it now resides in a parallel universe” (Askin). Reasons are mostly courteously provided. In haphazart! a relatively small core of around twelve group members regularly contribute to the group discussion board. A curious aspect of this communication is that even though participants present visually with a ‘buddy icon’ and most with a screen name not their real name, it is usual practice to address each other in discussions by their real Christian names, even when this is not evident in a member’s profile. This seems to indicate a common desire for authenticity. The makeup of the core varies from time to time depending on other activities in a member’s life. Although one or two may be professionally or semi-professionally engaged as photographers or artists or academics, most of these people would likely consider themselves to be “serious amateurs” (Cox). They are internationally dispersed with bias to the US, UK, Europe and Australia. English is the common language though not the natural tongue of some. The age range is approximately 35 to 65 and the gender mix 50/50. The group is three years old. Where Do We Go to from Here? In early January 2009 the haphazart! core was sparked into a frenzy of discussion by a post from a member headed “Where do we go to from here?” A proposal was mooted to produce a ‘book’ featuring images and texts representative of the group. Within three days a new public group with invited membership dedicated to the idea had been established. A smaller working party then retreated to a private Flickr group. Four months later Issue One of haphazart! magazine was available in print-on-demand and online formats. Following however is a brief critically reflective review of some of the collaborative curatorial, editorial and production processes for Issue Two which commenced in early June 2009. Most of the team had also been involved with Issue One. I was the only newcomer and replaced the person who had undertaken the design for Issue One. I was not provided access to the prior private editorial ruminations but apparently the collaborative curatorial and editorial decision-making practices the group had previously established persisted, and these took place entirely within the discussion forums of a new dedicated private Flickr group. Over a five-month period there were 1066 posts in 54 discussions concerning matters such as: change of format from the previous; selection of themes, artists and images; conduct of and editing of interviews; authoring of texts; copyright and reproduction. The idiom of those communications can be described as: discursive, sporadic, idiosyncratic, resourceful, collegial, cooperative, emphatic, earnest and purposeful. The selection process could not be said to follow anything close to a shared manifesto, or articulation of style. It was established that there would be two primary themes: the square format and contributors’ use of colour. Selection progressed by way of visual presentation and counter presentation until some kind of consensus was reached often involving informal votes of preference. Stretching the Limits of the Flickr Social Tools The magazine editorial collaborators continue to use the facilities with which they are familiar from regular Flickr group participation. However, the strict vertically linear format of the Flickr discussion format is particularly unsuited to lengthy, complex, asynchronous, multithreaded discussion. For this purpose it causes unnecessary strain, fatigue and confusion. Where images are included, the forums have set and maximum display sizes and are not flexibly configured into matrixes. Images cannot readily be communally changed or moved about like texts in a wiki. Likewise, the Flickrmail facility is of limited use for specialist editorial processes. Attachments cannot be added. This opinion expressed by a collaborator in the initial, open discussion for Issue One prevailed among Issue Two participants: do we want the members to go to another site to observe what is going on with the magazine? if that’s ok, then using google groups or something like that might make sense; if we want others to observe (and learn from) the process - we may want to do it here [in Flickr]. (Valentine) The opinion appears socially constructive; but because the final editorial process and production processes took place in a separate private forum, ultimately the suggested learning between one issue and the next did not take place. During Issue Two development the reluctance to try other online collaboration tools for the selection processes requiring visual comparative evaluation of images and trials of sequencing adhered. A number of ingenious methods of working within Flickr were devised and deployed and, in my opinion, proved frustratingly impractical and inefficient. The digital layout, design, collation and formatting of images and texts, all took place on my personal computer using professional software tools. Difficulties arose in progressively sharing this work for the purposes of review, appraisal and proofing. Eventually I ignored protests and insisted the team review demonstrations I had converted for sharing in Google Documents. But, with only one exception, I could not tempt collaborators to try commenting or editing in that environment. For example, instead of moving the sequence of images dynamically themselves, or even typing suggestions directly into Google Documents, they would post responses in Flickr. To Share and to Hold From the first imaginings of Issue One the need to have as an outcome something in one’s hands was expressed and this objective is apparently shared by all in the haphazart! core as an ongoing imperative. Various printing options have been nominated, discussed and evaluated. In the end one print-on-demand provider was selected on the basis of recommendation. The ethos of haphazart! is clearly not profit-making and conflicts with that of the printing organisation. Presumably to maintain an incentive to purchase the print copy online preview is restricted to the first 15 pages. To satisfy the co-requisite to make available the full 120 pages for free online viewing a second host that specialises in online presentation of publications is also utilised. In this way haphazart! members satisfy their common desires for sharing selected visual content and ideas with an online special interest audience and, for a physical object of art to relish—with all the connotations of preciousness, fetish, talisman, trophy, and bookish notions of haptic pleasure and visual treasure. The irony of publishing a frozen chunk of the ever-flowing Flickriver, whose temporally changing nature is arguably one of its most interesting qualities, is not a consideration. Most of them profess to be simply satisfying their own desire for self expression and would eschew any critical judgement as to whether this anarchic and discursive mode of operation results in a coherent statement about contemporary photographic abstraction. However there remains a distinct possibility that a number of core haphazart!ists aspire to transcend: popular taste; the discernment encouraged in camera clubs; and, the rhetoric of those involved professionally (Bourdieu et al.); and seek to engage with the “awareness of illegitimacy and the difficulties implied by the constitution of photography as an artistic medium” (Chamboredon 130). Incoherence: A Technical Note My personal experience of photography ranges from the filmic to the digital (Holmes, "Bridging Adelaide"). For a number of years I specialised in facsimile graphic reproduction of artwork. In those days I became aware that films were ‘blind’ to the psychophysical affect of some few particular paint pigments. They just could not be reproduced. Even so, as I handled the dozens of images contributed to haphazart!2, converting them from the pixellated place where Flickr exists to the resolution and gamut of the ink based colour space of books, I was surprised at the number of hue values that exist in the former that do not translate into the latter. In some cases the affect is subtle so that judicious tweaking of colour levels or local colour adjustment will satisfy discerning comparison between the screenic original and the ‘soft proof’ that simulates the printed outcome. In other cases a conversion simply does not compute. I am moved to contemplate, along with Harrison and Bartell (op. cit.) just how much of the experience of media in the shared digital space is incomparably new? Acknowledgement Acting on the advice of researchers experienced in cyberethnography (Bruckman; Suler, "Ethics") I have obtained the consent of co-collaborators to comment freely on proceedings that took place in a private forum. They have been given the opportunity to review and suggest changes to the account. References Askin, Dean (aka: dnskct). “Hall of Inappropriate.” Abstract Photos/Discuss/Hall of Inappropriate, 2010. 12 Jan. 2010 ‹http://www.flickr.com/groups/abstractphotos/discuss/72157623148695254/>. Bourdieu, Pierre, Luc Boltanski, Robert Castel, Jean-Claude Chamboredeon, and Dominique Schnapper. Photography: A Middle-Brow Art. 1965. Trans. Shaun Whiteside. Stanford: Stanford UP, 1990. Bruckman, Amy. Studying the Amateur Artist: A Perspective on Disguising Data Collected in Human Subjects Research on the Internet. 2002. 12 Jan. 2010 ‹http://www.nyu.edu/projects/nissenbaum/ethics_bru_full.html>. Bruns, Axel. “Towards Produsage: Futures for User-Led Content Production.” Proceedings: Cultural Attitudes towards Communication and Technology 2006. Perth: Murdoch U, 2006. 275–84. ———, and Mark Bahnisch. Social Media: Tools for User-Generated Content. Vol. 1 – “State of the Art.” Sydney: Smart Services CRC, 2009. Cha, Meeyoung, Alan Mislove, Ben Adams, and Krishna P. Gummadi. “Characterizing Social Cascades in Flickr.” Proceedings of the First Workshop on Online Social Networks. ACM, 2008. 13–18. ———, Alan Mislove, and Krishna P. Gummadi. “A Measurement-Driven Analysis of Information Propagation in the Flickr Social Network." WWW '09: Proceedings of the 18th International Conference on World Wide Web. ACM, 2009. 721–730. Cox, A.M., P.D. Clough, and J. Marlow. “Flickr: A First Look at User Behaviour in the Context of Photography as Serious Leisure.” Information Research 13.1 (March 2008). 12 Dec. 2009 ‹http://informationr.net/ir/13-1/paper336.html>. Chamboredon, Jean-Claude. “Mechanical Art, Natural Art: Photographic Artists.” Photography: A Middle-Brow Art. Pierre Bourdieu. et al. 1965. Trans. Shaun Whiteside. Stanford: Stanford UP, 1990. 129–149. Davies, Julia. “Display, Identity and the Everyday: Self-Presentation through Online Image Sharing.” Discourse: Studies in the Cultural Politics of Education 28.4 (Dec. 2007): 549–564. Elliott, Mark. “Stigmergic Collaboration: The Evolution of Group Work.” M/C Journal 9.2 (2006). 12 Jan. 2010 ‹http://journal.media-culture.org.au/0605/03-elliott.php>. Harrison, Teresa, M., and Brea Barthel. “Wielding New Media in Web 2.0: Exploring the History of Engagement with the Collaborative Construction of Media Products.” New Media & Society 11.1-2 (2009): 155–178. Holmes, Ashley. “‘Bridging Adelaide 2001’: Photography and Hyperimage, Spanning Paradigms.” VSMM 2000 Conference Proceedings. International Society for Virtual Systems and Multimedia, 2000. 79–88. ———. “Reconciling Experimentum and Experientia: Reflective Practice Research Methodology for the Creative Industries”. Speculation & Innovation: Applying Practice-Led Research in the Creative Industries. Brisbane: QUT, 2006. Kennedy, Lyndon, Mor Naaman, Shane Ahern, Rahul Nair, and Tye Rattenbury. “How Flickr Helps Us Make Sense of the World: Context and Content in Community-Contributed Media Collections.” MM’07. ACM, 2007. Miller, Andrew D., and W. Keith Edwards. “Give and Take: A Study of Consumer Photo-Sharing Culture and Practice.” Proceedings of the SIGCHI Conference on Human Factors in Computing Systems. ACM, 2007. 347–356. Mislove, Alan, Hema Swetha Koppula, Krishna P. Gummadi, Peter Druschel and Bobby Bhattacharjee. “Growth of the Flickr Social Network.” Proceedings of the First Workshop on Online Social Networks. ACM, 2008. 25–30. Negoescu, Radu-Andrei, and Daniel Gatica-Perez. “Analyzing Flickr Groups.” CIVR '08: Proceedings of the 2008 International Conference on Content-Based Image and Video Retrieval. ACM, 2008. 417–426. ———, Brett Adams, Dinh Phung, Svetha Venkatesh, and Daniel Gatica-Perez. “Flickr Hypergroups.” MM '09: Proceedings of the Seventeenth ACM International Conference on Multimedia. ACM, 2009. 813–816. Pink, Sarah. Doing Visual Ethnography: Images, Media and Representation in Research. 2nd ed. London: Sage, 2007. Su, Ja-Hwung, Bo-Wen Wang, Hsin-Ho Yeh, and Vincent S. Tseng. “Ontology–Based Semantic Web Image Retrieval by Utilizing Textual and Visual Annotations.” 2009 IEEE/WIC/ACM International Conference on Web Intelligence and Intelligent Agent Technology – Workshops. 2009. Suler, John. “Ethics in Cyberspace Research: Consent, Privacy and Contribution.” The Psychology of Cyberspace. 1996. 12 Jan. 2010 ‹http://www-usr.rider.edu/~suler/psycyber/psycyber.html>. ———. “Image, Word, Action: Interpersonal Dynamics in a Photo-Sharing Community.” Cyberpsychology & Behavior 11.5 (2008): 555–560. Valentine, Mark. “HAPHAZART! Magazine/Discuss/image selections…” [discussion post]. 2009. 12 Jan. 2010 ‹http://www.flickr.com/groups/haphazartmagazin/discuss/72157613147017532/>. Xu, Hongtao, Xiangdong Zhou, Mei Wang, Yu Xiang, and Baile Shi. “Exploring Flickr’s Related Tags for Semantic Annotation of Web Images.” CIVR ’09. ACM, 2009.

The first uncomfortable twinges started when I was a grad student, churning out my Master’s thesis on a laptop that I worked on at the library, in my bedroom, on the kitchen table, and at the coffee shop. By the last few months, typing was becoming uncomfortable for my arms, but as any thesis writer will tell you, your whole body is uncomfortable with the endless hours sitting, inputting, and revising. I didn’t think much of it until I moved on to a new city to start a PhD program. Now the burning that accompanied my essay-typing binges started to worry me more, especially since I noticed the twinges didn’t go away when I got up to chat with my roommate, or to go to bed. I finally mentioned the annoying arm to Sonja, a medical student friend of mine visiting me one afternoon. She asked me to pick up a chair in front of me, palms out. I did, and the attempt stabbed pain up my arm and through my elbow joint. The chair fell out of my hands. We looked at each other, eyebrows raised.Six months and much computer work later, I still hadn’t really addressed the issue. Who had time? Chasing mystery ailments around and more importantly, doing any less typing were not high on my likely list. But like the proverbial frog in slowly heated water, things had gotten much worse without my really acknowledging it. That is, until the day I got up from my laptop, stretched out and wandered into the kitchen to put some pasta on to boil. When the spaghetti was ready, I grabbed the pot to drain it and my right arm gave as if someone had just handed me a 200-pound weight. The pot, pasta and boiling water hit the floor with a scalding splash that nearly missed both me and the fleeing cat. Maybe there was a problem here.Both popular and critical understandings of the body have been in a great deal of flux over the past three or four decades as digital media technologies have become ever more pervasive and personal. Interfacing with the popular Internet, video games, mobile devices, wearable computing, and other new media technologies have prompted many to reflect on and reconsider what it means to be an embodied human being in an increasingly digitally determined era. As a result, the body, at various times in this recent history, has been theoretically disowned, disavowed, discarded, disdained, replaced, idealised, essentialised, hollowed out, re-occupied, dismembered, reconstituted, reclaimed and re-imagined in light of new media. Despite all of the angst over the relationships our embodied selves have had to digital media, of course, our embodied selves have endured. It remains true, that “even in the age of technosocial subjects, life is lived through bodies” (Stone 113).How we understand our embodiments and their entanglements with technologies matter deeply, moreover, for these understandings shape not only discourse around embodiment and media, but also the very bodies and media in question in very real ways. For example, a long-held tenet in both popular culture and academic work has been the notion that media technologies extend our bodies and our senses as technological prostheses. The idea here is that media technologies work like prostheses that extend the reach of our eyes, ears, voice, touch, and other bodily abilities through time and space, augmenting our abilities to experience and influence the world.Canadian media scholar Marshall McLuhan is one influential proponent of this notion, and claimed that, in fact, “the central purpose of all my work is to convey this message, that by understanding media as they extend man, we gain a measure of control over them” (McLuhan and Zingrone 265). Other more contemporary media scholars reflect on how “our prosthetic technological extensions enable us to amplify and extend ourselves in ways that profoundly affect the nature and scale of human communication” (Cleland 75), and suggest that a media technology such as one’s mobile device, can act “as a prosthesis that supports the individual in their interactions with the world” (Glitsos 161). Popular and commercial discourses also frequently make use of this idea, from the 1980’s AT&T ad campaign that nudged you to “Reach out and Touch Someone” via the telephone, to Texas Instruments’s claim in the 1990’s that their products were “Extending Your Reach”, to Nikon’s contemporary nudge to “See Much Further” with the prosthetic assistance of their cameras. The etymology of the term “prosthesis” reveals that the term evolves from Greek and Latin components that mean, roughly, “to add to”. The word was originally employed in the 16th century in a grammatical context to indicate “the addition of a letter or syllable to the beginning of a word”, and was adopted to describe “the replacement of defective or absent parts of the body by artificial substitutes” in the 1700’s. More recently the world “prosthesis” has come to be used to indicate more simply, “an artificial replacement for a part of the body” (OED Online). As we see in the use of the term over the past few decades, the meaning of the word continues to shift and is now often used to describe technological additions that don’t necessarily replace parts of the body, but augment and extend embodied capabilities in various ways. Technology as prosthesis is “a trope that has flourished in a recent and varied literature concerned with interrogating human-technology interfaces” (Jain 32), and now goes far beyond signifying the replacement of missing components. Although the prosthesis has “become somewhat of an all-purpose metaphor for interactions of body and technology” (Sun 16) and “a tempting theoretical gadget” (Jain 49), I contend that this metaphor is not often used particularly faithfully. Instead of invoking anything akin to the complex lived corporeal experiences and conundrums of prosthetic users, what we often get when it comes to metaphors of technology-as-prostheses is a fascination with the potential of technologies in seamlessly extending our bodies. This necessitates a fantasy version of both the body and its prostheses as interchangeable or extendable appendages to be unproblematically plugged and unplugged, modifying our capabilities and perceptions to our varying whims.Of course, a body seamlessly and infinitely extended by technological prostheses is really no body. This model forgoes actual lived bodies for a shiny but hollow amalgamation based on what I have termed the “disembodimyth” enabled by technological transcendence. By imagining our bodies as assemblages of optional appendages, it is not far of a leap to imagine opting out of our bodies altogether and using technological means to unfasten our consciousness from our corporeal parts. Alison Muri points out that this myth of imminent emancipation from our bodies via unity with technology is a view that has become “increasingly prominent in popular media and cultural studies” (74), despite or perhaps because of the fact that, due to global overpopulation and wasteful human environmental practices, “the human body has never before been so present, or so materially manifest at any time in the history of humanity”, rendering “contradictory, if not absurd, the extravagantly metaphorical claims over the past two decades of the human body’s disappearance or obsolescence due to technology” (75-76). In other words, it becomes increasingly difficult to speak seriously about the body being erased or escaped via technological prosthetics when those prosthetics, and our bodies themselves, continue to proliferate and contribute to the piling up of waste and pollution in the current Anthropocene. But whether they imply smooth couplings with alluring technologies, or uncoupling from the body altogether, these technology-as-prosthesis metaphors tell us very little about “prosthetic realities” (Sun 24). Actual prosthetic realities involve learning curves; pain, frustrations and triumphs; hard-earned remappings of mental models; and much experimentation and adaption on the part of both technology and user in order to function. In this vein, Vivian Sobchak has detailed the complex sensations and phenomenological effects that followed the amputation of her leg high above the knee, including the shifting presence of her “phantom limb” perceptions, the alignments, irritations, movements, and stabilities offered by her prosthetic leg, and her shifting senses of bodily integrity and body-image over time. An oversimplistic application of the prosthetic metaphor for our encounters with technology runs the risk of forgetting this wealth of experiences and instructive first-hand accounts from people who have been using therapeutic prosthetics as long as assistive devices have been conceived of, built, and used. Of course, prosthetics have long been employed not simply to aid function and mobility, but also to restore and prop up concepts of what a “whole,” “normal” body looks like, moves like, and includes as essential components. Prosthetics are employed, in many cases, to allow the user to “pass” as able-bodied in rendering their own technological presence invisible, in service of restoring an ableist notion of embodied normality. Scholars of Critical Disability Studies have pushed back against these ableist notions, in service of recognising the capacities of “the disabled body when it is understood not as a less than perfect form of the normative standard, but as figuring difference in a nonbinary sense” (Shildrick 14). Paralympian, actress, and model Aimee Mullins has lent her voice to this cause, publicly contesting the prioritisation of realistic, unobtrusive form in prosthetic design. In a TED talk entitled It’s Not Fair Having 12 Pairs of Legs, she showcases her collection of prosthetics, including “cheetah legs” designed for optimal running speed, transparent glass-like legs, ornately carved wooden legs, Barbie doll-inspired legs customised with high heel shoes, and beautiful, impractical jellyfish legs. In illustrating the functional, fashionable, and fantastical possibilities, she challenges prosthetic designers to embrace more poetry and whimsy, while urging us all to move “away from the need to replicate human-ness as the only aesthetic ideal” (Mullins). In this same light, Sarah S. Jain asks “how do body-prosthesis relays transform individual bodies as well as entire social notions about what a properly functioning physical body might be?” (39). In her exploration of how prostheses can be simultaneously wounding and enabling, Jain recounts Sigmund Freud’s struggle with his own palate replacement following surgery for throat cancer in 1923. His prosthesis allowed him to regain the ability to speak and eat, but also caused him significant pain. Nevertheless, his artificial palate had to be worn, or the tissue would shrink and necessitate additional painful procedures (Jain 31). Despite this fraught experience, Freud himself espoused the trope of technologically enhanced transcendence, pronouncing “Man has, as it were, become a prosthetic god. When he puts on all his auxiliary organs, he is truly magnificent.” However, he did add a qualification, perhaps reflective of his own experiences, by next noting, “but those organs have not grown on him and they still give him much trouble at times” (qtd. in Jain 31). This trouble is, I argue, important to remember and reclaim. It is also no less present in our interactions with our media prostheses. Many of our technological encounters with media come with unacknowledged discomforts, adjustments, lag, strain, ill-fitting defaults, and fatigue. From carpal tunnel syndrome to virtual reality vertigo, our interactions with media technologies are often marked by pain and “much trouble” in Freud’s sense. Computer Science and Cultural Studies scholar Phoebe Sengers opens a short piece titled Technological Prostheses: An Anecdote, by reflecting on how “we have reached the post-physical era. On the Internet, all that matters is our thoughts. The body is obsolete. At least, whoever designed my computer interface thought so.” She traces how concentrated interactions with computers during her graduate work led to intense tendonitis in her hands. Her doctor responded by handing her “a technological prosthesis, two black leather wrist braces” that allowed her to return to her keyboard to resume typing ten hours a day. Shortly after her assisted return to her computer, she developed severe tendonitis in her elbows and had to stop typing altogether. Her advisor also handed her a technological prosthesis, this time “a speech understanding system that would transcribe my words,” so that she could continue to work. Two days later she lost her voice. Ultimately she “learned that my body does not go away when I work. I learned to stop when it hurt […] and to refuse to behave as though my body was not there” (Sengers). My own experiences in grad school were similar in many ways to Sengers’s. Besides the pasta problem outlined above, my own computer interfacing injuries at that point in my career meant I could no longer turn keys in doors, use a screwdriver, lift weights, or play the guitar. I held a friend’s baby at Christmas that year and the pressure of the small body on my arm make me wince. My family doctor bent my arm around a little, then shrugging her shoulders, she signed me up for a nerve test. As a young neurologist proceeded to administer a series of electric shocks and stick pins into my arms in various places, I noticed she had an arm brace herself. She explained that she also had a repetitive strain injury aggravated by her work tasks. She pronounced mine an advanced repetitive strain injury involving both medial and lateral epicondylitis, and sent me home with recommendations for rest, ice and physiotherapy. Rest was a challenge: Like Sengers, I puzzled over how one might manage to be productive in academia without typing. I tried out some physiotherapy, with my arm connected to electrodes and currents coursing through my elbow until my arm contorted in bizarre ways involuntarily. I tried switching my mouse from my right side to my left, switching from typing to voice recognition software and switching from a laptop to a more ergonomic desktop setup. I tried herbal topical treatments, wearing an extremely ugly arm brace, doing yoga poses, and enduring chiropractic bone-cracking. I learned in talking with people around me at that time that repetitive strains of various kinds are surprisingly common conditions for academics and other computer-oriented occupations. I learned other things well worth learning in that painful process. In terms of my own writing and thinking about technology, I have even less tolerance for the idea of ephemeral, transcendent technological fusions between human and machine. Seductive slippages into a cyberspatial existence seem less sexy when bumping your body up against the very physical and unforgiving interface hurts more with each keystroke or mouse click. The experience has given me a chronic injury to manage carefully ever since, rationing my typing time and redoubling my commitment to practicing embodied theorising about technology, with attention to sensation, materiality, and the way joints (between bones or between computer and computant) can become points of inflammation. Although pain is rarely referenced in the myths of smooth human and technological incorporations, there is much to be learned in acknowledging and exploring the entry and exit wounds made when we interface with technology. The elbow, or wrist, or lower back, or mental health that gives out serves as an effective alarm, should it be ignored too long. If nothing else, like a crashed computer, a point of pain will break a flow of events typically taken for granted. Whether it is your screen or your pinky finger that unexpectedly freezes, a system collapse will prompt a step back to look with new perspective at the process you were engaged in. The lag, crash, break, gap, crack, or blister exposes the inherent imperfections in a system and offers up an invitation for reflection, critical engagement, and careful choice.One careful choice we could make would be a more critical engagement with technology-as-prosthesis by “re-membering” our jointedness with technologies. Of course, joints themselves are not distinct parts, but interesting articulated systems and relationships in the spaces in-between. Experiencing our jointedness with technologies involves recognising that this is not the smooth romantic union with technology that has so often been exalted. Instead, our technological articulations involve a range of pleasures and pain, flows and blockages, frictions and slippages, flexibilities and rigidities. I suggest that a new model for understanding technology and embodiment might employ “articulata” as a central figure, informed by the multiple meanings of articulation. At their simplest, articulata are hinged, jointed, plural beings, but they are also precarious things that move beyond a hollow collection of corporeal parts. The inspiration for an exploration of articulation as a metaphor in this way was planted by the work of Donna Haraway, and especially by her 1992 essay, “The Promises of Monsters: A Regenerative Politics for Inappropriate/d Others,” in which she touches briefly on articulation and its promise. Haraway suggests that “To articulate is to signify. It is to put things together, scary things, risky things, contingent things. I want to live in an articulate world. We articulate; therefore we are” (324). Following from Haraway’s work, this framework insists that bodies and technologies are not simply components cobbled together, but a set of relations that rework each other in complex and ongoing processes of articulation. The double-jointed meaning of articulation is particularly apt as inspiration for crafting a more nuanced understanding of embodiment, since articulation implies both physiology and communication. It is a term that can be used to explain physical jointedness and mobility, but also expressive specificities. We articulate a joint by exploring its range of motion and we articulate ideas by expressing them in words. In both senses we articulate and are articulated by our jointed nature. Instead of oversimplifying or idealising embodied relationships with prostheses and other technologies, we might conceive of them and experience them as part of a “joint project”, based on points of connexion that are not static, but dynamic, expressive, complex, contested, and sometimes uncomfortable. After all, as Shildrick reminds us, in addition to functioning as utilitarian material artifacts, “prostheses are rich in semiotic meaning and mark the site where the disordering ambiguity, and potential transgressions, of the interplay between the human, animal and machine cannot be occluded” (17). By encouraging the attentive embracing of these multiple meanings, disorderings, ambiguities, transgressions and interplays, my aim moving forward is to explore the ways in which we might all become more articulate about our articulations. After all, I too want to live in an articulate world.ReferencesAT&T. "AT&T Reach Out and Touch Someone Commercial – 1987." Advertisement. 13 Mar. 2014. 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Resources currently available to the scientific community that may be of interest for endocrinology research are described briefly here. More information is available through The Endocrine Society Home Page (http://www.endo-society.org) or the information provided below. Human Tissue and Biologic Specimen Resources NCI - Cooperative Human Tissue Network (CHTN) The NCI Cooperative Human Tissue Network (CHTN) provides normal, benign, precancerous, and cancerous human tissue to the scientific community for biomedical research. Specimens are collected according to the investigator’s individual protocol. Information provided with the specimens includes routine histopathologic and demographic data. The CHTN can also provide a variety of tissue microarrays. Contact the CHTN Web site at http://www-chtn.ims.nci.nih.gov, or 1-866-GO2-CHTN (1-866-462-2486). NCI - Cooperative Breast Cancer Tissue Resource (CBCTR) The NCI Cooperative Breast Cancer Tissue Resource (CBCTR) can provide researchers with access to formalin-fixed, paraffin-embedded primary breast cancer specimens, with associated pathologic, clinical, and outcome data. All specimens are evaluated for pathologic diagnosis by CBCTR pathologists using standard diagnostic criteria. The collection is particularly well suited for validation studies of diagnostic and prognostic markers. The CBCTR also makes available breast cancer tissue microarrays designed by NCI statisticians to provide high statistical power for studies of stage-specific markers of breast cancer. Contact CBCTR’s Web site at http://cbctr.nci.nih.gov, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770;marrouliss@imsweb.com. NCI - Cooperative Prostate Cancer Tissue Resource (CPCTR) The NCI Cooperative Prostate Cancer Tissue Resource (CPCTR) can provide access to over 4,000 cases of formalin-fixed, paraffin-embedded primary prostate cancer specimens, with associated pathology and clinical data. Fresh-frozen tissue is also available with limited clinical follow-up information. In addition, slides from prostate cancer tissue microarrays with associated pathology and clinical data are now available. Contact the CPCTR Web site at http://www.prostatetissues.org, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770;marrouliss@imsweb.com. NCI - AIDS and Cancer Specimen Resource (ACSR) The AIDS and Cancer Specimen Resource (ACSR) provides qualified researchers with tissue, cell, blood, and fluid specimens, as well as clinical data from patients with AIDS and cancer. The specimens and clinical data are available for research studies, particularly those that translate basic research findings to clinical application. Contact the ACSR Web site (http://acsr.ucsf.edu/) or Dr. Kishor Bhatia, (301) 496-7147;bhatiak@mail.nih.gov. NCI - Breast and Ovarian Cancer Family Registries (CFRs) The Breast and Ovarian CFRs facilitate and support interdisciplinary and population-based research on the identification and characterization of breast and ovarian cancer susceptibility genes, with particular emphasis on gene-gene and gene-environment interaction research. Available from the registries are: a) family history, epidemiologic and clinical data, b) updates on cancer recurrence, morbidity and mortality in participating families, and c) biospecimens, including plasma, lymphocytes, serum, DNA, Guthrie cards or buccal smears, and paraffin blocks of tumor tissue. For further information on these registries, contact the CFR Web site (http://epi.grants.cancer.gov/BCFR) or (301) 496-9600. NCI - Specimen Resource Locator The NCI Specimen Resource Locator (http://cancer.gov/specimens) is a database that helps researchers locate specimens for research. The database includes resources such as tissue banks and tissue procurement systems with access to normal, benign, precancerous, and/or cancerous human tissue covering a wide variety of organ sites. Researchers specify the types of specimens, number of cases, preservation methods, and associated data they require. The Locator will search the database and return a list of tissue resources most likely to meet their requirements. When no match is obtained, the researcher is referred to the NCI Tissue Expediter [(301) 496-7147;tissexp@mail.nih.gov]. The Tissue Expediter is a scientist who can help match researchers with appropriate resources or identify appropriate collaborators when those are necessary. NIDDK - Biologic Samples from Diabetic Study Foundation A portion (1/3) of all stored nonrenewable samples (plasma, serum, urine) from subjects enrolled in the Diabetes Control and Complications Trial (DCCT) is available for use by the scientific community to address questions for which these samples may be invaluable. Announcements for using this resource appear in the NIH Guide for Grants and Contracts periodically. Inquiries may be addressed to: Catherine C. Cowie, Ph.D., Director, Diabetes Epidemiology Program, NIDDK, 6707 Democracy Blvd., Room 691, MSC 5460, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892-5460. Phone: (301) 594-8804; fax: (301) 480-3503;cowiec@extra.niddk.nih.gov. NIDDK - NIDDK Central Repositories (Diabetes Prevention Study) The NIDDK Central Repositories have selected biosamples from the DPT-1 (The Diabetes Prevention Type 1) study that are available to qualified investigators through an application process. These samples are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. Information about how to apply for these materials can be obtained from the NIDDK Central Repositories by contacting Ms. Helen Ray of RTI, 1-919-316-3418, or hmp@rti.org. Direct scientific-technical inquiry to the Project Officer of the NIDDK Central Repositories, Dr. Rebekah Rasooly, at phone: (301) 594-6007;rr185i@nih.gov. Visit the Repositories Web site at http://www.niddkrepository.org. NICHD - Brain and Tissue Bank for Developmental Disorders The purpose of the Bank is to collect, preserve, and distribute human tissues to investigators interested in autism and developmental disorders; normal tissues may be available for other research purposes. Further information can be obtained at www.btbank.org. The contact persons are H. Ron Zielke or Sally Wisniewsky, University of Maryland (1-800-847-1539), and Carol Petito or Stephanie Lojko, University of Miami (1-800-592-7246). NICHD - Reproductive Tissue Sample Repository (RTSaR) The Reproductive Tissue Sample Repository (RTSaR) is a virtual repository with online tissue sample acquisition capabilities. The RTSaR provides investigators with real-time access to human and nonhuman primate tissue and fluid inventories from four tissue bank facilities that are supported through the Specialized Cooperative Centers Program in Reproduction Research. The tissue banks are located at the University of California, San Diego (human ovary bank), Stanford University (human endometrium and DNA bank), Johns Hopkins University (male reproductive tissues and fluids), and the Oregon National Primate Research Center (nonhuman primate tissues). The web site for the RTSaR is https://rtsar.nichd.nih.gov/rtsar/login. If you wish to access the RTSaR, you can request an id and password to access the system by contacting the network administrator at RTSaR@mail.nih.gov. Once you access the system, contact information for each bank is provided. Access is open to all investigators living in North America who are supported by research and research training grants from the NIH. One id and password will be provided to each principal investigator that can be utilized by any person working in the P.I.’s laboratory, or, in the case of institutional training grants (T32) and institutional career development award programs (K12), any person supported by the aforementioned awards. NCRR - Human Tissues and Organs Resource (HTOR) The Human Tissues and Organs Resource (HTOR) cooperative agreement supports a procurement network developed by the National Disease Research Interchange (NDRI), a not-for-profit organization. By collaborating with various medical centers, hospitals, pathology services, eye banks, tissue banks, and organ procurement organizations, HTOR provides a wide variety of human tissues and organs—both diseased and normal—to researchers for laboratory studies. Such samples include tissues from the central nervous system and brain, cardiovascular system, endocrine system, eyes, bone, and cartilage. For further information, consult the NDRI Web site (www.ndri.com) or contact Dr. John T. Lonsdale at NDRI, 8 Penn Center, 8th Floor, 1628 JFK Boulevard, Philadelphia, PA 19103. Phone: (800) 222-6374, ext. 271; fax: (215) 557-7154;jlonsdale@ndriresource.org. The NDRI Web site is http://www.ndri.com. NCRR - Islet Cell Resource (ICR) With support from NCRR, 10 Islet Cell Resource (ICR) centers isolate, purify, and characterize human pancreatic islets for subsequent transplantation into patients with type I diabetes. The ICR centers procure whole pancreata and acquire relevant data about donors; improve islet isolation and purification techniques; distribute islets for use in approved clinical protocols; and perfect the methods of storage and shipping. In this way, the centers optimize the viability, function, and availability of islets and help clinical researchers capitalize on the recently reported successes in islet transplantation. Information on submitting requests for islet cells can be obtained from Mr. John Kaddis, ICR Coordinating Center Project Manager, City of Hope National Medical Center, 1500 E. Duarte Road, Duarte, California 91010. Phone (626) 359-8111, ext. 63377; fax: (626) 471-7106;jkaddis@coh.org. The Coordinating Center hosts a Web site at http://icr.coh.org. NIA - SWAN Repository (longitudinal, multiethnic study of women at midlife including the menopausal transition) The SWAN Repository is a biologic specimen bank of the Study of Women’s Health Across the Nation (SWAN). The SWAN cohort was recruited in 1996/1997 and consists of 3302 African-American, Caucasian, Chinese, Hispanic, and Japanese women. The SWAN Repository contains more than 350,000 blood and urine specimens generated from the study participants’ annual visits (8 visits to date), at which time medical and health history, psychosocial measures, biological measures, and anthropometric data were and are being collected. In addition, a subset of the participants are providing urine samples, collected daily over the length of one menstrual cycle, each year. More than 900,000 of these samples are in the SWAN Repository and are available to researchers who wish to study the midlife and menopausal transition. Additionally, a DNA sample repository is also available and includes DNA as well as transformed B-lymphoblastoid cell lines from more than 1800 of the participants. To learn more about the SWAN Repository and how to apply to use SWAN Repository specimens, contact the Web site at http://www.swanrepository.com or Dr. MaryFran Sowers, University of Michigan, School of Public Health, Epidemiology Dept., (734) 936-3892;mfsowers@umich.edu. Human and Animal Cell and Biologic Reagent Resources NIDDK - National Hormone and Peptide Program The National Hormone and Peptide Program (NHPP) offers peptide hormones and their antisera, tissues (rat hypothalami), and miscellaneous reagents to qualified investigators. These reagents are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. These materials can be obtained from Dr. A. F. Parlow of the Harbor-UCLA Medical Center, Research and Education Institute, Torrance, CA. A more complete description of resources within this program is provided in The Endocrine Society journals. Direct scientific-technical inquiry to NHPP Scientific Director, Dr. Al Parlow, at phone: (310) 222-3537; fax: (310) 222-3432;parlow@humc.edu. Visit the NHPP Web site at http://www.humc.edu/hormones. NICHD - National Hormone and Pituitary Program (see NIDDK listing) Following is a list of reagents currently available through the resources of NICHD:Androgen receptor and peptide antigen Recombinant monkey (cynomolgus) and baboon luteinizing hormone and follicle-stimulating hormone and antisera. NIA - Aging Cell Bank To facilitate aging research on cells in culture, the NIA provides support for the Aging Cell Bank located at the Coriell Institute for Medical Research in Camden, NJ. The Aged Cell Bank provides fibroblast, lymphoblastoid, and differentiated cell lines from a wide range of human age-related conditions and other mammalian species, as well as DNA from a limited subset of cell lines. For further information, the Aged Cell Bank catalog can be accessed at http://locus.umdnj.edu/nia or contact Dr. Donald Coppock at 1-800-752-3805. NCRR - Various Cell Repositories NCRR maintains the following cell repository resources: National Cell Culture Center, National Stem Cell Resource, and the Yeast Genetic Stock Center. Further information regarding these resources may be obtained through the NCRR Web site at: www.ncrr.nih.gov/ncrrprog/cmpdir/BIOLOG.asp. Animal Resources NIA - Aging Rodent Resources NIA maintains both rat and mouse colonies for use by the scientific community. The animals available range in age from 1 to 36 months. A repository of fresh-frozen tissue from the NIA aged rodent colonies is stocked with tissue from mouse and rat strains, including caloric-restricted BALB/c mice. The NIA also maintains a colony of calorically restricted rodents of selected genotypes, which are available to the scientific community. For further information, please refer to the Aged Rodent information handbook at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentColoniesHandbook/ or contact the Office of Biological Resources and Resource Development order desk. Phone: (301) 496-0181; fax: (301) 402-5597;rodents@nia.nih.gov. NIA - Aged Rodent Tissue Bank The rodent tissue bank contains flash-frozen tissues from rodents in the NIA aged rodent colonies. Tissue is collected from rodents at 4 or 5 age points throughout the lifespan. Tissue arrays are also available. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentTissueBankHandbook/. NCRR - Mutant Mouse Regional Resource Centers (MMRRC) The Mutant Mouse Regional Resource Center (MMRRC) Program consists of centers that collectively operate as a one-stop shop to serve the biomedical research community. Investigators who have created select mutant mouse models may donate their models to an MMRRC for broad dissemination to other investigators who request them for noncommercial research investigations related to human health, disease, and treatments. The NCRR Division of Comparative Medicine (DCM) supports the MMRRCs, which are electronically linked through the MMRRC Informatics Coordinating Center (ICC) to function as one facility. The ICC, located at The Jackson Laboratory in Bar Harbor, ME, provides database and other informatics support to the MMRRC to give the research community a single entry point to the program. Further information can be obtained from the Web site at http://www.mmrrc.org, or from Franziska Grieder, D.V.M., Ph.D., Division of Comparative Medicine, NCRR. Phone (301) 435-0744; fax: (301) 480-3819;griederf@ncrr.nih.gov. NCRR - Induced Mutant Mouse Resource (IMR) The Induced Mutant Mouse Resource (IMR) at The Jackson Laboratory provides researchers with genetically engineered mice (transgenic, targeted mutant, retroviral insertional mutant, and chemically induced mutant mice). The function of the IMR is to select, import, cryopreserve, maintain, and distribute these important strains of mice to the research community. To improve their value for research, the IMR also undertakes genetic development of stocks, such as transferring mutant genes or transgenes to defined genetic backgrounds and combining transgenes and/or targeted mutations to create new mouse models for research. Over 800 mutant stocks have been accepted by the IMR. Current holdings include models for research on cancer, immunological and inflammatory diseases, neurological diseases and behavioral disorders, cardiovascular diseases, developmental disorders, metabolic and other diseases, reporter (e.g. GFP) and recombinase (e.g. cre/loxP) strains. About 8 strains a month are being added to the IMR holdings. A list of all strains may be obtained from the IMR Web site: www.jax.org/resources/documents/imr/. Online submission forms are also available on that site. All mice can be ordered by calling The Jackson Laboratory’s Customer Service Department at 1-800-422-MICE or (207) 288-5845 or by faxing (207) 288-6150. NIDDK - Mouse Metabolic Phenotyping Centers The mission of the Mouse Metabolic Phenotyping Centers is to provide the scientific community with standardized, high-quality metabolic and physiologic phenotyping services for mouse models of diabetes, diabetic complications, obesity, and related disorders. Researchers can ship mice to one of the four Centers (University of Cincinnati, University of Texas Southwestern Medical Center, Vanderbilt University, and Yale University) and obtain on a fee-for-service basis a range of complex exams used to characterize mouse metabolism, blood composition, energy balance, eating and exercise, organ function and morphology, physiology, and histology. Many tests are done in living animals and are designed to elucidate the subtle hallmarks of metabolic disease. Information, including a complete list of available tests, can be found at www.mmpc.org, or contact Dr. Maren R. Laughlin, NIDDK, at (301) 594-8802;Maren.Laughlin@nih.gov; or Dr. Kristin Abraham, NIDDK, at (301) 451-8048;abrahamk@extra.niddk.nih.gov. NCRR - National Primate Research Centers (NPRCs) National Primate Research Centers (NPRCs) are a network of eight highly specialized facilities for nonhuman primates (NHP) research. Funded by grants through NCRR’s Division of Comparative Medicine (DCM), each center, staffed with experienced research and support staff, provides the appropriate research environment to foster the development of NHP models of human health and disease for biomedical investigations. The NPRCs are affiliated with academic institutions and are accessible to eligible biomedical and behavioral investigators supported by research project grants from the National Institutes of Health and other sources. Further information may be obtained from the notice, Procedures for Accessing Regional Primate Research Centers, published in the NIH Guide for Grants and Contracts at http://grants2.nih.gov/grants/guide/notice-files/not97-014.html, or from John Harding, Ph.D., National Primate Research Centers and AIDS Animal Models Program, Division of Comparative Medicine, NCRR. Phone: (301) 435-0744; fax: (301) 480-3819;hardingj@mail.nih.gov. NIA - Nonhuman Primates, Aging Set-Aside Colony NIA maintains approximately 200 nonhuman primates (M. mulatta) at four National Primate Research Centers (see above) for conducting research on aging. These animals range in age from 18 to 35 years. While these animals are predominantly reserved for non-invasive research, exceptions can be made to this policy. For further information, please contact Dr. Nancy Nadon, Office of Biological Resources and Resource Development, NIA. Phone: (301) 402-7744; fax: (301) 402-0010;nadonn@nia.nih.gov. NIA - Nonhuman Primate (NHP) Tissue Bank and Aging Database The NIA developed two new resources to facilitate research in the NHP model. The NHP tissue bank contains fresh-frozen and fixed tissue donated by primate centers around the country. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/NHPTissueBankHandbook.htm. The Primate Aging Database provides an internet accessible database with data from thousands of primates around the country. It can be used to investigate the effect of age on a variety of parameters, predominantly blood chemistry and husbandry measurements. The site is password protected. The URL is http://ipad.primate.wisc.edu. NIA - Obesity, Diabetes and Aging Animal Resource (USF-ODARC) The NIA supports a colony of aged rhesus macaques, many of which are obese and/or diabetic. This is a long-term colony of monkeys housed at the University of South Florida’s Obesity, Diabetes and Aging Research Center. They have been extensively and longitudinally characterized for general health variables, blood chemistry, food intake, and body weight. Diabetic monkeys are tested daily for urine glucose and ketone levels, and prediabetic monkeys are tested weekly. Data for some of the monkeys extend as far back as 15 years. This unique resource is available for collaborative studies. ODARC has a significant amount of stored tissue collected at necropsy and stored blood/plasma collected longitudinally. Serial blood collection or tissue collection at necropsy can also be performed prospectively. Testing and imaging can also be performed on the monkeys. Inquiries regarding collaborative studies using the ODARC colony should be directed to: Barbara C. Hansen, Ph.D., Director, Obesity, Diabetes and Aging Research Center, University of South Florida, All Children’s Hospital, 801 6th Street South #9340, St. Petersburg, FL 33701. Phone: (727) 767-6993; fax: (727) 767-7443;bchansen@aol.com. NCRR - Various Animal Resources NCRR maintains the following animal resources: Animal Models and Genetic Stocks, Chimpanzee Biomedical Research Program, NIH Animal Genetic Resource, and the Specific Pathogen Free Macaque Breeding and Research Program. Further information regarding these and other resources may be obtained through the NCRR Web site at www.ncrr.nih.gov/comparative_med.asp. IN SILICO RESOURCES NIDDK, NHLBI, and NIEHS - Nuclear Receptor Signaling Atlas The Nuclear Receptor Signaling Atlas (NURSA) has created an in silico resource comprised of curated information about Nuclear Receptors, Coregulators, Ligands, and Downstream Targets. NURSA is sponsored by NIH and provides online access through a public webportal at www.NURSA.org. Ease of navigation through a series of molecule pages allows users to make queries about Nuclear Receptors, Coactivators and Corepressors. Additional information about nuclear receptor ligands is provided, as well as primary datasets relating to expression profiling of nuclear receptors, coregulators and downstream targets. The molecule pages are hyperlinked to data contained in external databases, including NCBI, KEGG, UniProt, and others, allowing for detailed data mining. In partnership with The Endocrine Society, NURSA and Molecular Endocrinology (http://mend.endojournals.org/) have reciprocal links designed to enhance publications in Molecular Endocrinology and the information available through the NURSA molecule pages. Links to additional relevant literature citations are from PubMed at the National Library of Medicine. Miscellaneous Resources NCRR - National Gene Vector Laboratories (NGVLs) The National Gene Vector Laboratories (NGVLs), with core funding from NCRR, serve as a resource for researchers to obtain adequate quantities of clinical-grade vectors for human gene transfer protocols. The vector types include retrovirus, lentivirus, adenovirus, adeno-associated virus, herpes-virus, and DNA plasmids. The NGVLs consist of three vector production centers at: Baylor College of Medicine; City of Hope National Medical Center and Beckman Research Institute; and Indiana University, which also serves as the Coordinating Center for all the laboratories. Two additional laboratories conduct toxicology studies for NGVL-approved investigators. These laboratories are located at the Southern Research Institute and the University of Florida. Additional information about the process for requesting vector production and/or pharmacology/toxicology support should be directed to Ms. Lorraine Matheson, NGVL Project Coordinator, Indiana University School of Medicine. Phone: (317) 274-4519; fax: (317) 278-4518;lrubin@iupui.edu. The NGVL Coordinating Center at Indiana University also hosts a Web site at http://www.ngvl.org. NCRR - General Clinical Research Centers (GCRCs) The General Clinical Research Centers (GCRCs) are a national network of 82 centers that provide optimal settings for medical investigators to conduct safe, controlled, state-of-the-art in-patient and out-patient studies of both children and adults. GCRCs also provide infrastructure and resources that support several career development opportunities. Investigators who have research project funding from the National Institutes of Health (NIH) and other peer-reviewed sources may apply to use GCRCs. Because the GCRCs support a full spectrum of patient-oriented scientific inquiry, researchers who use these centers can benefit from collaborative, multidisciplinary research opportunities. To request access to a GCRC facility, eligible investigators should initially contact a GCRC program director, listed in the National Center for Research Resources (NCRR) Clinical Research Resources Directory (www.ncrr.nih.gov/ncrrprog/clindir/crdirectory.asp). Further information can be obtained from Anthony R. Hayward, M.D., Director, Division for Clinical Research Resources, National Center for Research Resources at NIH. Phone: (301) 435-0790;haywarda@ncrr.nih.gov.

Resources currently available to the scientific community that may be of interest for endocrinology research are described briefly here. More information is available through The Endocrine Society Home Page (http://www.endo-society.org) or the information provided below. HUMAN TISSUE AND BIOLOGIC SPECIMEN RESOURCES NCI - Cooperative Human Tissue Network (CHTN) The NCI Cooperative Human Tissue Network (CHTN) provides normal, benign, precancerous, and cancerous human tissue to the scientific community for biomedical research. Specimens are collected according to the investigator’s individual protocol. Information provided with the specimens includes routine histopathologic and demographic data. The CHTN can also provide a variety of tissue microarrays. Contact the CHTN Web site at http://www-chtn.ims.nci.nih.gov, or 1-866-GO2-CHTN (1-866-462-2486). NCI - Cooperative Breast Cancer Tissue Resource (CBCTR) The NCI Cooperative Breast Cancer Tissue Resource (CBCTR) can provide researchers with access to formalin-fixed, paraffin-embedded primary breast cancer specimens, with associated pathologic, clinical, and outcome data. All specimens are evaluated for pathologic diagnosis by CBCTR pathologists using standard diagnostic criteria. The collection is particularly well suited for validation studies of diagnostic and prognostic markers. The CBCTR also makes available breast cancer tissue microarrays designed by NCI statisticians to provide high statistical power for studies of stage-specific markers of breast cancer. Contact CBCTR’s Web site at http://cbctr.nci.nih.gov, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com. NCI - Cooperative Prostate Cancer Tissue Resource (CPCTR) The NCI Cooperative Prostate Cancer Tissue Resource (CPCTR) can provide access to over 4,000 cases of formalin-fixed, paraffin-embedded primary prostate cancer specimens, with associated pathology and clinical data. Fresh-frozen tissue is also available with limited clinical follow-up information. In addition, slides from prostate cancer tissue microarrays with associated pathology and clinical data are now available. Contact the CPCTR Web site at http://www.prostatetissues.org, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com. NCI - AIDS and Cancer Specimen Resource (ACSR) The AIDS and Cancer Specimen Resource (ACSR) provides qualified researchers with tissue, cell, blood, and fluid specimens, as well as clinical data from patients with AIDS and cancer. The specimens and clinical data are available for research studies, particularly those that translate basic research findings to clinical application. Contact the ACSR Web site (http://acsr.ucsf.edu/) or Dr. Kishor Bhatia, (301) 496-7147; e-mail: bhatiak@mail.nih.gov. NCI - Breast and Ovarian Cancer Family Registries (CFRs) The Breast and Ovarian CFRs facilitate and support interdisciplinary and population-based research on the identification and characterization of breast and ovarian cancer susceptibility genes, with particular emphasis on gene-gene and gene-environment interaction research. Available from the registries are: a) family history, epidemiologic and clinical data, b) updates on cancer recurrence, morbidity and mortality in participating families, and c) biospecimens, including plasma, lymphocytes, serum, DNA, Guthrie cards or buccal smears, and paraffin blocks of tumor tissue. For further information on these registries, contact the CFR Web site (http://epi.grants.cancer.gov/BCFR) or (301) 496-9600. NCI - Specimen Resource Locator The NCI Specimen Resource Locator (http://cancer.gov/specimens) is a database that helps researchers locate specimens for research. The database includes resources such as tissue banks and tissue procurement systems with access to normal, benign, precancerous, and/or cancerous human tissue covering a wide variety of organ sites. Researchers specify the types of specimens, number of cases, preservation methods, and associated data they require. The Locator will search the database and return a list of tissue resources most likely to meet their requirements. When no match is obtained, the researcher is referred to the NCI Tissue Expediter [(301) 496-7147; e-mail: tissexp@mail.nih.gov]. The Tissue Expediter is a scientist who can help match researchers with appropriate resources or identify appropriate collaborators when those are necessary. NIDDK - Biologic Samples from Diabetic Study Foundation A portion (1/3) of all stored nonrenewable samples (plasma, serum, urine) from subjects enrolled in the Diabetes Control and Complications Trial (DCCT) is available for use by the scientific community to address questions for which these samples may be invaluable. Announcements for using this resource appear in the NIH Guide for Grants and Contracts periodically. Inquiries may be addressed to: Catherine C. Cowie, Ph.D., Director, Diabetes Epidemiology Program, NIDDK, 6707 Democracy Blvd., Room 691, MSC 5460, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892-5460. Phone: (301) 594-8804; fax: (301) 480-3503; e-mail: cowiec@extra.niddk.nih.gov. NIDDK - NIDDK Central Repositories (Diabetes Prevention Study) The NIDDK Central Repositories have selected biosamples from the DPT-1 (The Diabetes Prevention Type 1) study that are available to qualified investigators through an application process. These samples are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. Information about how to apply for these materials can be obtained from the NIDDK Central Repositories by contacting Ms. Helen Ray of RTI, 1-919-316-3418, or hmp@rti.org. Direct scientific-technical inquiry to the Project Officer of the NIDDK Central Repositories, Dr. Rebekah Rasooly, at phone: (301) 594-6007; e-mail: rr185i@nih.gov. Visit the Repositories Web site at http://www.niddkrepository.org. NICHD - Brain and Tissue Bank for Developmental Disorders The purpose of the Bank is to collect, preserve, and distribute human tissues to investigators interested in autism and developmental disorders; normal tissues may be available for other research purposes. Further information can be obtained at www.btbank.org. The contact persons are H. Ron Zielke or Sally Wisniewsky, University of Maryland (1-800-847-1539), and Carol Petito or Stephanie Lojko, University of Miami (1-800-592-7246). NICHD - Reproductive Tissue Sample Repository (RTSaR) The Reproductive Tissue Sample Repository (RTSaR) is a virtual repository with online tissue sample acquisition capabilities. The RTSaR provides investigators with real-time access to human and nonhuman primate tissue and fluid inventories from four tissue bank facilities that are supported through the Specialized Cooperative Centers Program in Reproduction Research. The tissue banks are located at the University of California, San Diego (human ovary bank), Stanford University (human endometrium and DNA bank), Johns Hopkins University (male reproductive tissues and fluids), and the Oregon National Primate Research Center (nonhuman primate tissues). The web site for the RTSaR is https://rtsar.nichd.nih.gov/rtsar/login. If you wish to access the RTSaR, you can request an id and password to access the system by contacting the network administrator at RTSaR@mail.nih.gov. Once you access the system, contact information for each bank is provided. Access is open to all investigators living in North America who are supported by research and research training grants from the NIH. One id and password will be provided to each principal investigator that can be utilized by any person working in the P.I.’s laboratory, or, in the case of institutional training grants (T32) and institutional career development award programs (K12), any person supported by the aforementioned awards. NCRR - Human Tissues and Organs Resource (HTOR) The Human Tissues and Organs Resource (HTOR) cooperative agreement supports a procurement network developed by the National Disease Research Interchange (NDRI), a not-for-profit organization. By collaborating with various medical centers, hospitals, pathology services, eye banks, tissue banks, and organ procurement organizations, HTOR provides a wide variety of human tissues and organs—both diseased and normal—to researchers for laboratory studies. Such samples include tissues from the central nervous system and brain, cardiovascular system, endocrine system, eyes, bone, and cartilage. For further information, consult the NDRI Web site (www.ndri.com) or contact Dr. John T. Lonsdale at NDRI, 8 Penn Center, 8th Floor, 1628 JFK Boulevard, Philadelphia, PA 19103. Phone: (800) 222-6374, ext. 271; fax: (215) 557-7154; e-mail: jlonsdale@ndriresource.org. The NDRI Web site is http://www.ndri.com. NCRR - Islet Cell Resource (ICR) With support from NCRR, 10 Islet Cell Resource (ICR) centers isolate, purify, and characterize human pancreatic islets for subsequent transplantation into patients with type I diabetes. The ICR centers procure whole pancreata and acquire relevant data about donors; improve islet isolation and purification techniques; distribute islets for use in approved clinical protocols; and perfect the methods of storage and shipping. In this way, the centers optimize the viability, function, and availability of islets and help clinical researchers capitalize on the recently reported successes in islet transplantation. Information on submitting requests for islet cells can be obtained from Mr. John Kaddis, ICR Coordinating Center Project Manager, City of Hope National Medical Center, 1500 E. Duarte Road, Duarte, California 91010. Phone (626) 359-8111, ext. 63377; fax: (626) 471-7106; e-mail: jkaddis@coh.org. The Coordinating Center hosts a Web site at http://icr.coh.org. NIA - SWAN Repository (longitudinal, multiethnic study of women at midlife including the menopausal transition) The SWAN Repository is a biologic specimen bank of the Study of Women’s Health Across the Nation (SWAN). The SWAN cohort was recruited in 1996/1997 and consists of 3302 African-American, Caucasian, Chinese, Hispanic, and Japanese women. The SWAN Repository contains more than 350,000 blood and urine specimens generated from the study participants’ annual visits (8 visits to date), at which time medical and health history, psychosocial measures, biological measures, and anthropometric data were and are being collected. In addition, a subset of the participants are providing urine samples, collected daily over the length of one menstrual cycle, each year. More than 900,000 of these samples are in the SWAN Repository and are available to researchers who wish to study the midlife and menopausal transition. Additionally, a DNA sample repository is also available and includes DNA as well as transformed B-lymphoblastoid cell lines from more than 1800 of the participants. To learn more about the SWAN Repository and how to apply to use SWAN Repository specimens, contact the Web site at http://www.swanrepository.com or Dr. MaryFran Sowers, University of Michigan, School of Public Health, Epidemiology Dept., (734) 936-3892; e-mail: mfsowers@umich.edu. HUMAN AND ANIMAL CELL AND BIOLOGIC REAGENT RESOURCES NIDDK - National Hormone and Peptide Program The National Hormone and Peptide Program (NHPP) offers peptide hormones and their antisera, tissues (rat hypothalami), and miscellaneous reagents to qualified investigators. These reagents are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. These materials can be obtained from Dr. A. F. Parlow of the Harbor-UCLA Medical Center, Research and Education Institute, Torrance, CA. A more complete description of resources within this program is provided in The Endocrine Society journals. Direct scientific-technical inquiry to NHPP Scientific Director, Dr. Al Parlow, at phone: (310) 222-3537; fax: (310) 222-3432; e-mail: parlow@humc.edu. Visit the NHPP Web site at http://www.humc.edu/hormones. NICHD - National Hormone and Pituitary Program (see NIDDK listing) Following is a list of reagents currently available through the resources of NICHD: Androgen receptor and peptide antigenRecombinant monkey (cynomolgus) and baboon luteinizing hormone and follicle-stimulating hormone and antisera. NIA - Aging Cell Bank To facilitate aging research on cells in culture, the NIA provides support for the Aging Cell Bank located at the Coriell Institute for Medical Research in Camden, NJ. The Aged Cell Bank provides fibroblast, lymphoblastoid, and differentiated cell lines from a wide range of human age-related conditions and other mammalian species, as well as DNA from a limited subset of cell lines. For further information, the Aged Cell Bank catalog can be accessed at http://locus.umdnj.edu/nia or contact Dr. Donald Coppock at 1-800-752-3805. NCRR - Various Cell Repositories NCRR maintains the following cell repository resources: National Cell Culture Center, National Stem Cell Resource, and the Yeast Genetic Stock Center. Further information regarding these resources may be obtained through the NCRR Web site at: www.ncrr.nih.gov/ncrrprog/cmpdir/BIOLOG.asp. ANIMAL RESOURCES NIA - Aging Rodent Resources NIA maintains both rat and mouse colonies for use by the scientific community. The animals available range in age from 1 to 36 months. A repository of fresh-frozen tissue from the NIA aged rodent colonies is stocked with tissue from mouse and rat strains, including caloric-restricted BALB/c mice. The NIA also maintains a colony of calorically restricted rodents of selected genotypes, which are available to the scientific community. For further information, please refer to the Aged Rodent information handbook at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentColoniesHandbook/ or contact the Office of Biological Resources and Resource Development order desk. Phone: (301) 496-0181; fax: (301) 402-5597; e-mail: rodents@nia.nih.gov. NIA - Aged Rodent Tissue Bank The rodent tissue bank contains flash-frozen tissues from rodents in the NIA aged rodent colonies. Tissue is collected from rodents at 4 or 5 age points throughout the lifespan. Tissue arrays are also available. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentTissueBankHandbook/. NCRR - Mutant Mouse Regional Resource Centers (MMRRC) The Mutant Mouse Regional Resource Center (MMRRC) Program consists of centers that collectively operate as a one-stop shop to serve the biomedical research community. Investigators who have created select mutant mouse models may donate their models to an MMRRC for broad dissemination to other investigators who request them for noncommercial research investigations related to human health, disease, and treatments. The NCRR Division of Comparative Medicine (DCM) supports the MMRRCs, which are electronically linked through the MMRRC Informatics Coordinating Center (ICC) to function as one facility. The ICC, located at The Jackson Laboratory in Bar Harbor, ME, provides database and other informatics support to the MMRRC to give the research community a single entry point to the program. Further information can be obtained from the Web site at http://www.mmrrc.org, or from Franziska Grieder, D.V.M., Ph.D., Division of Comparative Medicine, NCRR. Phone (301) 435-0744; fax: (301) 480-3819; e-mail: griederf@ncrr.nih.gov. NCRR - Induced Mutant Mouse Resource (IMR) The Induced Mutant Mouse Resource (IMR) at The Jackson Laboratory provides researchers with genetically engineered mice (transgenic, targeted mutant, retroviral insertional mutant, and chemically induced mutant mice). The function of the IMR is to select, import, cryopreserve, maintain, and distribute these important strains of mice to the research community. To improve their value for research, the IMR also undertakes genetic development of stocks, such as transferring mutant genes or transgenes to defined genetic backgrounds and combining transgenes and/or targeted mutations to create new mouse models for research. Over 800 mutant stocks have been accepted by the IMR. Current holdings include models for research on cancer, immunological and inflammatory diseases, neurological diseases and behavioral disorders, cardiovascular diseases, developmental disorders, metabolic and other diseases, reporter (e.g. GFP) and recombinase (e.g. cre/loxP) strains. About 8 strains a month are being added to the IMR holdings. A list of all strains may be obtained from the IMR Web site: www.jax.org/resources/documents/imr/. Online submission forms are also available on that site. All mice can be ordered by calling The Jackson Laboratory’s Customer Service Department at 1-800-422-MICE or (207) 288-5845 or by faxing (207) 288-6150. NIDDK - Mouse Metabolic Phenotyping Centers The mission of the Mouse Metabolic Phenotyping Centers is to provide the scientific community with standardized, high-quality metabolic and physiologic phenotyping services for mouse models of diabetes, diabetic complications, obesity, and related disorders. Researchers can ship mice to one of the four Centers (University of Cincinnati, University of Texas Southwestern Medical Center, Vanderbilt University, and Yale University) and obtain on a fee-for-service basis a range of complex exams used to characterize mouse metabolism, blood composition, energy balance, eating and exercise, organ function and morphology, physiology, and histology. Many tests are done in living animals and are designed to elucidate the subtle hallmarks of metabolic disease. Information, including a complete list of available tests, can be found at www.mmpc.org, or contact Dr. Maren R. Laughlin, NIDDK, at (301) 594-8802; e-mail: Maren.Laughlin@nih.gov; or Dr. Kristin Abraham, NIDDK, at (301) 451-8048; e-mail: abrahamk@extra.niddk.nih.gov. NCRR - National Primate Research Centers (NPRCs) National Primate Research Centers (NPRCs) are a network of eight highly specialized facilities for nonhuman primates (NHP) research. Funded by grants through NCRR’s Division of Comparative Medicine (DCM), each center, staffed with experienced research and support staff, provides the appropriate research environment to foster the development of NHP models of human health and disease for biomedical investigations. The NPRCs are affiliated with academic institutions and are accessible to eligible biomedical and behavioral investigators supported by research project grants from the National Institutes of Health and other sources. Further information may be obtained from the notice, Procedures for Accessing Regional Primate Research Centers, published in the NIH Guide for Grants and Contracts at http://grants2.nih.gov/grants/guide/notice-files/not97-014.html, or from John Harding, Ph.D., National Primate Research Centers and AIDS Animal Models Program, Division of Comparative Medicine, NCRR. Phone: (301) 435-0744; fax: (301) 480-3819; e-mail: hardingj@mail.nih.gov. NIA - Nonhuman Primates, Aging Set-Aside Colony NIA maintains approximately 200 nonhuman primates (M. mulatta) at four National Primate Research Centers (see above) for conducting research on aging. These animals range in age from 18 to 35 years. While these animals are predominantly reserved for non-invasive research, exceptions can be made to this policy. For further information, please contact Dr. Nancy Nadon, Office of Biological Resources and Resource Development, NIA. Phone: (301) 402-7744; fax: (301) 402-0010; e-mail: nadonn@nia.nih.gov. NIA - Nonhuman Primate (NHP) Tissue Bank and Aging Database The NIA developed two new resources to facilitate research in the NHP model. The NHP tissue bank contains fresh-frozen and fixed tissue donated by primate centers around the country. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/NHPTissueBankHandbook.htm. The Primate Aging Database provides an internet accessible database with data from thousands of primates around the country. It can be used to investigate the effect of age on a variety of parameters, predominantly blood chemistry and husbandry measurements. The site is password protected. The URL is http://ipad.primate.wisc.edu. NIA - Obesity, Diabetes and Aging Animal Resource (USF-ODARC) The NIA supports a colony of aged rhesus macaques, many of which are obese and/or diabetic. This is a long-term colony of monkeys housed at the University of South Florida’s Obesity, Diabetes and Aging Research Center. They have been extensively and longitudinally characterized for general health variables, blood chemistry, food intake, and body weight. Diabetic monkeys are tested daily for urine glucose and ketone levels, and prediabetic monkeys are tested weekly. Data for some of the monkeys extend as far back as 15 years. This unique resource is available for collaborative studies. ODARC has a significant amount of stored tissue collected at necropsy and stored blood/plasma collected longitudinally. Serial blood collection or tissue collection at necropsy can also be performed prospectively. Testing and imaging can also be performed on the monkeys. Inquiries regarding collaborative studies using the ODARC colony should be directed to: Barbara C. Hansen, Ph.D., Director, Obesity, Diabetes and Aging Research Center, University of South Florida, All Children’s Hospital, 801 6th Street South #9340, St. Petersburg, FL 33701. Phone: (727) 767-6993; fax: (727) 767-7443; e-mail: bchansen@aol.com. NCRR - Various Animal Resources NCRR maintains the following animal resources: Animal Models and Genetic Stocks, Chimpanzee Biomedical Research Program, NIH Animal Genetic Resource, and the Specific Pathogen Free Macaque Breeding and Research Program. Further information regarding these and other resources may be obtained through the NCRR Web site at www.ncrr.nih.gov/comparative_med.asp. IN SILICO RESOURCES NIDDK, NHLBI, and NIEHS - Nuclear Receptor Signaling Atlas The Nuclear Receptor Signaling Atlas (NURSA) has created an in silico resource comprised of curated information about Nuclear Receptors, Coregulators, Ligands, and Downstream Targets. NURSA is sponsored by NIH and provides online access through a public webportal at www.NURSA.org. Ease of navigation through a series of molecule pages allows users to make queries about Nuclear Receptors, Coactivators and Corepressors. Additional information about nuclear receptor ligands is provided, as well as primary datasets relating to expression profiling of nuclear receptors, coregulators and downstream targets. The molecule pages are hyperlinked to data contained in external databases, including NCBI, KEGG, UniProt, and others, allowing for detailed data mining. In partnership with The Endocrine Society, NURSA and Molecular Endocrinology (http://mend.endojournals.org/) have reciprocal links designed to enhance publications in Molecular Endocrinology and the information available through the NURSA molecule pages. Links to additional relevant literature citations are from PubMed at the National Library of Medicine. MISCELLANEOUS RESOURCES NCRR - National Gene Vector Laboratories (NGVLs) The National Gene Vector Laboratories (NGVLs), with core funding from NCRR, serve as a resource for researchers to obtain adequate quantities of clinical-grade vectors for human gene transfer protocols. The vector types include retrovirus, lentivirus, adenovirus, adeno-associated virus, herpes-virus, and DNA plasmids. The NGVLs consist of three vector production centers at: Baylor College of Medicine; City of Hope National Medical Center and Beckman Research Institute; and Indiana University, which also serves as the Coordinating Center for all the laboratories. Two additional laboratories conduct toxicology studies for NGVL-approved investigators. These laboratories are located at the Southern Research Institute and the University of Florida. Additional information about the process for requesting vector production and/or pharmacology/toxicology support should be directed to Ms. Lorraine Matheson, NGVL Project Coordinator, Indiana University School of Medicine. Phone: (317) 274-4519; fax: (317) 278-4518; e-mail: lrubin@iupui.edu. The NGVL Coordinating Center at Indiana University also hosts a Web site at http://www.ngvl.org. NCRR - General Clinical Research Centers (GCRCs) The General Clinical Research Centers (GCRCs) are a national network of 82 centers that provide optimal settings for medical investigators to conduct safe, controlled, state-of-the-art in-patient and out-patient studies of both children and adults. GCRCs also provide infrastructure and resources that support several career development opportunities. Investigators who have research project funding from the National Institutes of Health (NIH) and other peer-reviewed sources may apply to use GCRCs. Because the GCRCs support a full spectrum of patient-oriented scientific inquiry, researchers who use these centers can benefit from collaborative, multidisciplinary research opportunities. To request access to a GCRC facility, eligible investigators should initially contact a GCRC program director, listed in the National Center for Research Resources (NCRR) Clinical Research Resources Directory (www.ncrr.nih.gov/ncrrprog/clindir/crdirectory.asp). Further information can be obtained from Anthony R. Hayward, M.D., Director, Division for Clinical Research Resources, National Center for Research Resources at NIH. Phone: (301) 435-0790; e-mail: haywarda@ncrr.nih.gov.

Resources currently available to the scientific community that may be of interest for endocrinology research are described briefly here. More information is available through The Endocrine Society Home Page (http://www.endo-society.org) or the information provided below. Human Tissue and Biologic Specimen Resources NCI - Cooperative Human Tissue Network (CHTN) The NCI Cooperative Human Tissue Network (CHTN) provides normal, benign, precancerous, and cancerous human tissue to the scientific community for biomedical research. Specimens are collected according to the investigator’s individual protocol. Information provided with the specimens includes routine histopathologic and demographic data. The CHTN can also provide a variety of tissue microarrays. Contact the CHTN Web site at http://www-chtn.ims.nci.nih.gov, or 1-866-GO2-CHTN (1-866-462-2486). NCI - Cooperative Breast Cancer Tissue Resource (CBCTR) The NCI Cooperative Breast Cancer Tissue Resource (CBCTR) can provide researchers with access to formalin-fixed, paraffin-embedded primary breast cancer specimens, with associated pathologic, clinical, and outcome data. All specimens are evaluated for pathologic diagnosis by CBCTR pathologists using standard diagnostic criteria. The collection is particularly well suited for validation studies of diagnostic and prognostic markers. The CBCTR also makes available breast cancer tissue microarrays designed by NCI statisticians to provide high statistical power for studies of stage-specific markers of breast cancer. Contact CBCTR’s Web site at http://cbctr.nci.nih.gov, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com. NCI - Cooperative Prostate Cancer Tissue Resource (CPCTR) The NCI Cooperative Prostate Cancer Tissue Resource (CPCTR) can provide access to over 4,000 cases of formalin-fixed, paraffin-embedded primary prostate cancer specimens, with associated pathology and clinical data. Fresh-frozen tissue is also available with limited clinical follow-up information. In addition, slides from prostate cancer tissue microarrays with associated pathology and clinical data are now available. Contact the CPCTR Web site at http://www.prostatetissues.org, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com. NCI - AIDS and Cancer Specimen Resource (ACSR) The AIDS and Cancer Specimen Resource (ACSR) provides qualified researchers with tissue, cell, blood, and fluid specimens, as well as clinical data from patients with AIDS and cancer. The specimens and clinical data are available for research studies, particularly those that translate basic research findings to clinical application. Contact the ACSR Web site (http://acsr.ucsf.edu/) or Dr. Kishor Bhatia, (301) 496-7147; e-mail: bhatiak@mail.nih.gov. NCI - Breast and Ovarian Cancer Family Registries (CFRs) The Breast and Ovarian CFRs facilitate and support interdisciplinary and population-based research on the identification and characterization of breast and ovarian cancer susceptibility genes, with particular emphasis on gene-gene and gene-environment interaction research. Available from the registries are: a) family history, epidemiologic and clinical data, b) updates on cancer recurrence, morbidity and mortality in participating families, and c) biospecimens, including plasma, lymphocytes, serum, DNA, Guthrie cards or buccal smears, and paraffin blocks of tumor tissue. For further information on these registries, contact the CFR Web site (http://epi.grants.cancer.gov/BCFR) or (301) 496-9600. NCI - Specimen Resource Locator The NCI Specimen Resource Locator (http://cancer.gov/specimens) is a database that helps researchers locate specimens for research. The database includes resources such as tissue banks and tissue procurement systems with access to normal, benign, precancerous, and/or cancerous human tissue covering a wide variety of organ sites. Researchers specify the types of specimens, number of cases, preservation methods, and associated data they require. The Locator will search the database and return a list of tissue resources most likely to meet their requirements. When no match is obtained, the researcher is referred to the NCI Tissue Expediter [(301) 496-7147; e-mail: tissexp@mail.nih.gov]. The Tissue Expediter is a scientist who can help match researchers with appropriate resources or identify appropriate collaborators when those are necessary. NIDDK - Biologic Samples from Diabetic Study Foundation A portion (1/3) of all stored nonrenewable samples (plasma, serum, urine) from subjects enrolled in the Diabetes Control and Complications Trial (DCCT) is available for use by the scientific community to address questions for which these samples may be invaluable. Announcements for using this resource appear in the NIH Guide for Grants and Contracts periodically. Inquiries may be addressed to: Catherine C. Cowie, Ph.D., Director, Diabetes Epidemiology Program, NIDDK, 6707 Democracy Blvd., Room 691, MSC 5460, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892-5460. Phone: (301) 594-8804; fax: (301) 480-3503; e-mail: cowiec@extra.niddk.nih.gov. NIDDK - NIDDK Central Repositories (Diabetes Prevention Study) The NIDDK Central Repositories have selected biosamples from the DPT-1 (The Diabetes Prevention Type 1) study that are available to qualified investigators through an application process. These samples are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. Information about how to apply for these materials can be obtained from the NIDDK Central Repositories by contacting Ms. Helen Ray of RTI, 1-919-316-3418, or hmp@rti.org. Direct scientific-technical inquiry to the Project Officer of the NIDDK Central Repositories, Dr. Rebekah Rasooly, at phone: (301) 594-6007; e-mail: rr185i@nih.gov. Visit the Repositories Web site at http://www.niddkrepository.org. NICHD - Brain and Tissue Bank for Developmental Disorders The purpose of the Bank is to collect, preserve, and distribute human tissues to investigators interested in autism and developmental disorders; normal tissues may be available for other research purposes. Further information can be obtained at www.btbank.org. The contact persons are H. Ron Zielke or Sally Wisniewsky, University of Maryland (1-800-847-1539), and Carol Petito or Stephanie Lojko, University of Miami (1-800-592-7246). NICHD - Reproductive Tissue Sample Repository (RTSaR) The Reproductive Tissue Sample Repository (RTSaR) is a virtual repository with online tissue sample acquisition capabilities. The RTSaR provides investigators with real-time access to human and nonhuman primate tissue and fluid inventories from four tissue bank facilities that are supported through the Specialized Cooperative Centers Program in Reproduction Research. The tissue banks are located at the University of California, San Diego (human ovary bank), Stanford University (human endometrium and DNA bank), Johns Hopkins University (male reproductive tissues and fluids), and the Oregon National Primate Research Center (nonhuman primate tissues). The web site for the RTSaR is https://rtsar.nichd.nih.gov/rtsar/login. If you wish to access the RTSaR, you can request an id and password to access the system by contacting the network administrator at RTSaR@mail.nih.gov. Once you access the system, contact information for each bank is provided. Access is open to all investigators living in North America who are supported by research and research training grants from the NIH. One id and password will be provided to each principal investigator that can be utilized by any person working in the P.I.’s laboratory, or, in the case of institutional training grants (T32) and institutional career development award programs (K12), any person supported by the aforementioned awards. NCRR - Human Tissues and Organs Resource (HTOR) The Human Tissues and Organs Resource (HTOR) cooperative agreement supports a procurement network developed by the National Disease Research Interchange (NDRI), a not-for-profit organization. By collaborating with various medical centers, hospitals, pathology services, eye banks, tissue banks, and organ procurement organizations, HTOR provides a wide variety of human tissues and organs—both diseased and normal—to researchers for laboratory studies. Such samples include tissues from the central nervous system and brain, cardiovascular system, endocrine system, eyes, bone, and cartilage. For further information, consult the NDRI Web site (www.ndri.com) or contact Dr. John T. Lonsdale at NDRI, 8 Penn Center, 8th Floor, 1628 JFK Boulevard, Philadelphia, PA 19103. Phone: (800) 222-6374, ext. 271; fax: (215) 557-7154; e-mail: jlonsdale@ndriresource.org. The NDRI Web site is http://www.ndri.com. NCRR - Islet Cell Resource (ICR) With support from NCRR, 10 Islet Cell Resource (ICR) centers isolate, purify, and characterize human pancreatic islets for subsequent transplantation into patients with type I diabetes. The ICR centers procure whole pancreata and acquire relevant data about donors; improve islet isolation and purification techniques; distribute islets for use in approved clinical protocols; and perfect the methods of storage and shipping. In this way, the centers optimize the viability, function, and availability of islets and help clinical researchers capitalize on the recently reported successes in islet transplantation. Information on submitting requests for islet cells can be obtained from Mr. John Kaddis, ICR Coordinating Center Project Manager, City of Hope National Medical Center, 1500 E. Duarte Road, Duarte, California 91010. Phone (626) 359-8111, ext. 63377; fax: (626) 471-7106; e-mail: jkaddis@coh.org. The Coordinating Center hosts a Web site at http://icr.coh.org. NIA - SWAN Repository (longitudinal, multiethnic study of women at midlife including the menopausal transition) The SWAN Repository is a biologic specimen bank of the Study of Women’s Health Across the Nation (SWAN). The SWAN cohort was recruited in 1996/1997 and consists of 3302 African-American, Caucasian, Chinese, Hispanic, and Japanese women. The SWAN Repository contains more than 350,000 blood and urine specimens generated from the study participants’ annual visits (8 visits to date), at which time medical and health history, psychosocial measures, biological measures, and anthropometric data were and are being collected. In addition, a subset of the participants are providing urine samples, collected daily over the length of one menstrual cycle, each year. More than 900,000 of these samples are in the SWAN Repository and are available to researchers who wish to study the midlife and menopausal transition. Additionally, a DNA sample repository is also available and includes DNA as well as transformed B-lymphoblastoid cell lines from more than 1800 of the participants. To learn more about the SWAN Repository and how to apply to use SWAN Repository specimens, contact the Web site at http://www.swanrepository.com or Dr. MaryFran Sowers, University of Michigan, School of Public Health, Epidemiology Dept., (734) 936-3892; e-mail: mfsowers@umich.edu. Human and Animal Cell and Biologic Reagent Resources NIDDK - National Hormone and Peptide Program The National Hormone and Peptide Program (NHPP) offers peptide hormones and their antisera, tissues (rat hypothalami), and miscellaneous reagents to qualified investigators. These reagents are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. These materials can be obtained from Dr. A. F. Parlow of the Harbor-UCLA Medical Center, Research and Education Institute, Torrance, CA. A more complete description of resources within this program is provided in The Endocrine Society journals. Direct scientific-technical inquiry to NHPP Scientific Director, Dr. Al Parlow, at phone: (310) 222-3537; fax: (310) 222-3432; e-mail: parlow@humc.edu. Visit the NHPP Web site at http://www.humc.edu/hormones. NICHD - National Hormone and Pituitary Program (see NIDDK listing) Following is a list of reagents currently available through the resources of NICHD: Androgen receptor and peptide antigen Recombinant monkey (cynomolgus) and baboon luteinizing hormone and follicle-stimulating hormone and antisera. NIA - Aging Cell Bank To facilitate aging research on cells in culture, the NIA provides support for the Aging Cell Bank located at the Coriell Institute for Medical Research in Camden, NJ. The Aged Cell Bank provides fibroblast, lymphoblastoid, and differentiated cell lines from a wide range of human age-related conditions and other mammalian species, as well as DNA from a limited subset of cell lines. For further information, the Aged Cell Bank catalog can be accessed at http://locus.umdnj.edu/nia or contact Dr. Donald Coppock at 1-800-752-3805. NCRR - Various Cell Repositories NCRR maintains the following cell repository resources: National Cell Culture Center, National Stem Cell Resource, and the Yeast Genetic Stock Center. Further information regarding these resources may be obtained through the NCRR Web site at: www.ncrr.nih.gov/ncrrprog/cmpdir/BIOLOG.asp. ANIMAL RESOURCES NIA - Aging Rodent Resources NIA maintains both rat and mouse colonies for use by the scientific community. The animals available range in age from 1 to 36 months. A repository of fresh-frozen tissue from the NIA aged rodent colonies is stocked with tissue from mouse and rat strains, including caloric-restricted BALB/c mice. The NIA also maintains a colony of calorically restricted rodents of selected genotypes, which are available to the scientific community. For further information, please refer to the Aged Rodent information handbook at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentColoniesHandbook/ or contact the Office of Biological Resources and Resource Development order desk. Phone: (301) 496-0181; fax: (301) 402-5597; e-mail: rodents@nia.nih.gov. NIA - Aged Rodent Tissue Bank The rodent tissue bank contains flash-frozen tissues from rodents in the NIA aged rodent colonies. Tissue is collected from rodents at 4 or 5 age points throughout the lifespan. Tissue arrays are also available. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentTissueBankHandbook/. NCRR - Mutant Mouse Regional Resource Centers (MMRRC) The Mutant Mouse Regional Resource Center (MMRRC) Program consists of centers that collectively operate as a one-stop shop to serve the biomedical research community. Investigators who have created select mutant mouse models may donate their models to an MMRRC for broad dissemination to other investigators who request them for noncommercial research investigations related to human health, disease, and treatments. The NCRR Division of Comparative Medicine (DCM) supports the MMRRCs, which are electronically linked through the MMRRC Informatics Coordinating Center (ICC) to function as one facility. The ICC, located at The Jackson Laboratory in Bar Harbor, ME, provides database and other informatics support to the MMRRC to give the research community a single entry point to the program. Further information can be obtained from the Web site at http://www.mmrrc.org, or from Franziska Grieder, D.V.M., Ph.D., Division of Comparative Medicine, NCRR. Phone (301) 435-0744; fax: (301) 480-3819; e-mail: griederf@ncrr.nih.gov. NCRR - Induced Mutant Mouse Resource (IMR) The Induced Mutant Mouse Resource (IMR) at The Jackson Laboratory provides researchers with genetically engineered mice (transgenic, targeted mutant, retroviral insertional mutant, and chemically induced mutant mice). The function of the IMR is to select, import, cryopreserve, maintain, and distribute these important strains of mice to the research community. To improve their value for research, the IMR also undertakes genetic development of stocks, such as transferring mutant genes or transgenes to defined genetic backgrounds and combining transgenes and/or targeted mutations to create new mouse models for research. Over 800 mutant stocks have been accepted by the IMR. Current holdings include models for research on cancer, immunological and inflammatory diseases, neurological diseases and behavioral disorders, cardiovascular diseases, developmental disorders, metabolic and other diseases, reporter (e.g. GFP) and recombinase (e.g. cre/loxP) strains. About 8 strains a month are being added to the IMR holdings. A list of all strains may be obtained from the IMR Web site: www.jax.org/resources/documents/imr/. Online submission forms are also available on that site. All mice can be ordered by calling The Jackson Laboratory’s Customer Service Department at 1-800-422-MICE or (207) 288-5845 or by faxing (207) 288-6150. NIDDK - Mouse Metabolic Phenotyping Centers The mission of the Mouse Metabolic Phenotyping Centers is to provide the scientific community with standardized, high-quality metabolic and physiologic phenotyping services for mouse models of diabetes, diabetic complications, obesity, and related disorders. Researchers can ship mice to one of the four Centers (University of Cincinnati, University of Texas Southwestern Medical Center, Vanderbilt University, and Yale University) and obtain on a fee-for-service basis a range of complex exams used to characterize mouse metabolism, blood composition, energy balance, eating and exercise, organ function and morphology, physiology, and histology. Many tests are done in living animals and are designed to elucidate the subtle hallmarks of metabolic disease. Information, including a complete list of available tests, can be found at www.mmpc.org, or contact Dr. Maren R. Laughlin, NIDDK, at (301) 594-8802; e-mail: Maren.Laughlin@nih.gov; or Dr. Kristin Abraham, NIDDK, at (301) 451-8048; e-mail: abrahamk@extra.niddk.nih.gov. NCRR - National Primate Research Centers (NPRCs) National Primate Research Centers (NPRCs) are a network of eight highly specialized facilities for nonhuman primates (NHP) research. Funded by grants through NCRR’s Division of Comparative Medicine (DCM), each center, staffed with experienced research and support staff, provides the appropriate research environment to foster the development of NHP models of human health and disease for biomedical investigations. The NPRCs are affiliated with academic institutions and are accessible to eligible biomedical and behavioral investigators supported by research project grants from the National Institutes of Health and other sources. Further information may be obtained from the notice, Procedures for Accessing Regional Primate Research Centers, published in the NIH Guide for Grants and Contracts at http://grants2.nih.gov/grants/guide/notice-files/not97-014.html, or from John Harding, Ph.D., National Primate Research Centers and AIDS Animal Models Program, Division of Comparative Medicine, NCRR. Phone: (301) 435-0744; fax: (301) 480-3819; e-mail: hardingj@mail.nih.gov. NIA - Nonhuman Primates, Aging Set-Aside Colony NIA maintains approximately 200 nonhuman primates (M. mulatta) at four National Primate Research Centers (see above) for conducting research on aging. These animals range in age from 18 to 35 years. While these animals are predominantly reserved for non-invasive research, exceptions can be made to this policy. For further information, please contact Dr. Nancy Nadon, Office of Biological Resources and Resource Development, NIA. Phone: (301) 402-7744; fax: (301) 402-0010; e-mail: nadonn@nia.nih.gov. NIA - Nonhuman Primate (NHP) Tissue Bank and Aging Database The NIA developed two new resources to facilitate research in the NHP model. The NHP tissue bank contains fresh-frozen and fixed tissue donated by primate centers around the country. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/NHPTissueBankHandbook.htm. The Primate Aging Database provides an internet accessible database with data from thousands of primates around the country. It can be used to investigate the effect of age on a variety of parameters, predominantly blood chemistry and husbandry measurements. The site is password protected. The URL is http://ipad.primate.wisc.edu. NIA - Obesity, Diabetes and Aging Animal Resource (USF-ODARC) The NIA supports a colony of aged rhesus macaques, many of which are obese and/or diabetic. This is a long-term colony of monkeys housed at the University of South Florida’s Obesity, Diabetes and Aging Research Center. They have been extensively and longitudinally characterized for general health variables, blood chemistry, food intake, and body weight. Diabetic monkeys are tested daily for urine glucose and ketone levels, and prediabetic monkeys are tested weekly. Data for some of the monkeys extend as far back as 15 years. This unique resource is available for collaborative studies. ODARC has a significant amount of stored tissue collected at necropsy and stored blood/plasma collected longitudinally. Serial blood collection or tissue collection at necropsy can also be performed prospectively. Testing and imaging can also be performed on the monkeys. Inquiries regarding collaborative studies using the ODARC colony should be directed to: Barbara C. Hansen, Ph.D., Director, Obesity, Diabetes and Aging Research Center, University of South Florida, All Children’s Hospital, 801 6th Street South #9340, St. Petersburg, FL 33701. Phone: (727) 767-6993; fax: (727) 767-7443; e-mail: bchansen@aol.com. NCRR - Various Animal Resources NCRR maintains the following animal resources: Animal Models and Genetic Stocks, Chimpanzee Biomedical Research Program, NIH Animal Genetic Resource, and the Specific Pathogen Free Macaque Breeding and Research Program. Further information regarding these and other resources may be obtained through the NCRR Web site at www.ncrr.nih.gov/comparative_med.asp. In Silico Resources NIDDK, NHLBI, and NIEHS - Nuclear Receptor Signaling Atlas The Nuclear Receptor Signaling Atlas (NURSA) has created an in silico resource comprised of curated information about Nuclear Receptors, Coregulators, Ligands, and Downstream Targets. NURSA is sponsored by NIH and provides online access through a public webportal at www.NURSA.org. Ease of navigation through a series of molecule pages allows users to make queries about Nuclear Receptors, Coactivators and Corepressors. Additional information about nuclear receptor ligands is provided, as well as primary datasets relating to expression profiling of nuclear receptors, coregulators and downstream targets. The molecule pages are hyperlinked to data contained in external databases, including NCBI, KEGG, UniProt, and others, allowing for detailed data mining. In partnership with The Endocrine Society, NURSA and Molecular Endocrinology (http://mend.endojournals.org/) have reciprocal links designed to enhance publications in Molecular Endocrinology and the information available through the NURSA molecule pages. Links to additional relevant literature citations are from PubMed at the National Library of Medicine. Miscellaneous Resources NCRR - National Gene Vector Laboratories (NGVLs) The National Gene Vector Laboratories (NGVLs), with core funding from NCRR, serve as a resource for researchers to obtain adequate quantities of clinical-grade vectors for human gene transfer protocols. The vector types include retrovirus, lentivirus, adenovirus, adeno-associated virus, herpes-virus, and DNA plasmids. The NGVLs consist of three vector production centers at: Baylor College of Medicine; City of Hope National Medical Center and Beckman Research Institute; and Indiana University, which also serves as the Coordinating Center for all the laboratories. Two additional laboratories conduct toxicology studies for NGVL-approved investigators. These laboratories are located at the Southern Research Institute and the University of Florida. Additional information about the process for requesting vector production and/or pharmacology/toxicology support should be directed to Ms. Lorraine Matheson, NGVL Project Coordinator, Indiana University School of Medicine. Phone: (317) 274-4519; fax: (317) 278-4518; e-mail: lrubin@iupui.edu. The NGVL Coordinating Center at Indiana University also hosts a Web site at http://www.ngvl.org. NCRR - General Clinical Research Centers (GCRCs) The General Clinical Research Centers (GCRCs) are a national network of 82 centers that provide optimal settings for medical investigators to conduct safe, controlled, state-of-the-art in-patient and out-patient studies of both children and adults. GCRCs also provide infrastructure and resources that support several career development opportunities. Investigators who have research project funding from the National Institutes of Health (NIH) and other peer-reviewed sources may apply to use GCRCs. Because the GCRCs support a full spectrum of patient-oriented scientific inquiry, researchers who use these centers can benefit from collaborative, multidisciplinary research opportunities. To request access to a GCRC facility, eligible investigators should initially contact a GCRC program director, listed in the National Center for Research Resources (NCRR) Clinical Research Resources Directory (www.ncrr.nih.gov/ncrrprog/clindir/crdirectory.asp). Further information can be obtained from Anthony R. Hayward, M.D., Director, Division for Clinical Research Resources, National Center for Research Resources at NIH. Phone: (301) 435-0790; e-mail: haywarda@ncrr.nih.gov.

Resources currently available to the scientific community that may be of interest for endocrinology research are described briefly here. More information is available through The Endocrine Society Home Page (http://www.endo-society.org) or the information provided below. Human Tissue and Biologic Specimen Resources NCI - Cooperative Human Tissue Network (CHTN) The NCI Cooperative Human Tissue Network (CHTN) provides normal, benign, precancerous, and cancerous human tissue to the scientific community for biomedical research. Specimens are collected according to the investigator’s individual protocol. Information provided with the specimens includes routine histopathologic and demographic data. The CHTN can also provide a variety of tissue microarrays. Contact the CHTN Web site at http://www-chtn.ims.nci.nih.gov, or 1-866-GO2-CHTN (1-866-462-2486). NCI - Cooperative Breast Cancer Tissue Resource (CBCTR) The NCI Cooperative Breast Cancer Tissue Resource (CBCTR) can provide researchers with access to formalin-fixed, paraffin-embedded primary breast cancer specimens, with associated pathologic, clinical, and outcome data. All specimens are evaluated for pathologic diagnosis by CBCTR pathologists using standard diagnostic criteria. The collection is particularly well suited for validation studies of diagnostic and prognostic markers. The CBCTR also makes available breast cancer tissue microarrays designed by NCI statisticians to provide high statistical power for studies of stage-specific markers of breast cancer. Contact CBCTR’s Web site at http://cbctr.nci.nih.gov, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com NCI - Cooperative Prostate Cancer Tissue Resource (CPCTR) The NCI Cooperative Prostate Cancer Tissue Resource (CPCTR) can provide access to over 4,000 cases of formalin-fixed, paraffin-embedded primary prostate cancer specimens, with associated pathology and clinical data. Fresh-frozen tissue is also available with limited clinical follow-up information. In addition, slides from prostate cancer tissue microarrays with associated pathology and clinical data are now available. Contact the CPCTR Web site at http://www.prostatetissues.org, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com NCI - AIDS and Cancer Specimen Resource (ACSR) The AIDS and Cancer Specimen Resource (ACSR) provides qualified researchers with tissue, cell, blood, and fluid specimens, as well as clinical data from patients with AIDS and cancer. The specimens and clinical data are available for research studies, particularly those that translate basic research findings to clinical application. Contact the ACSR Web site (http://acsr.ucsf.edu/) or Dr. Kishor Bhatia, (301) 496-7147; e-mail: bhatiak@mail.nih.gov NCI - Breast and Ovarian Cancer Family Registries (CFRs) The Breast and Ovarian CFRs facilitate and support interdisciplinary and population-based research on the identification and characterization of breast and ovarian cancer susceptibility genes, with particular emphasis on gene-gene and gene-environment interaction research. Available from the registries are: a) family history, epidemiologic and clinical data, b) updates on cancer recurrence, morbidity and mortality in participating families, and c) biospecimens, including plasma, lymphocytes, serum, DNA, Guthrie cards or buccal smears, and paraffin blocks of tumor tissue. For further information on these registries, contact the CFR Web site (http://epi.grants.cancer.gov/BCFR) or (301) 496-9600. NCI - Specimen Resource Locator The NCI Specimen Resource Locator (http://cancer.gov/specimens) is a database that helps researchers locate specimens for research. The database includes resources such as tissue banks and tissue procurement systems with access to normal, benign, precancerous, and/or cancerous human tissue covering a wide variety of organ sites. Researchers specify the types of specimens, number of cases, preservation methods, and associated data they require. The Locator will search the database and return a list of tissue resources most likely to meet their requirements. When no match is obtained, the researcher is referred to the NCI Tissue Expediter [(301) 496-7147; e-mail: tissexp@mail.nih.gov]. The Tissue Expediter is a scientist who can help match researchers with appropriate resources or identify appropriate collaborators when those are necessary. NIDDK - Biologic Samples from Diabetic Study Foundation A portion (1/3) of all stored nonrenewable samples (plasma, serum, urine) from subjects enrolled in the Diabetes Control and Complications Trial (DCCT) is available for use by the scientific community to address questions for which these samples may be invaluable. Announcements for using this resource appear in the NIH Guide for Grants and Contracts periodically. Inquiries may be addressed to: Catherine C. Cowie, Ph.D., Director, Diabetes Epidemiology Program, NIDDK, 6707 Democracy Blvd., Room 691, MSC 5460, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892-5460. Phone: (301) 594-8804; fax: (301) 480-3503; e-mail: cowiec@extra.niddk.nih.gov NIDDK - NIDDK Central Repositories (Diabetes Prevention Study) The NIDDK Central Repositories have selected biosamples from the DPT-1 (The Diabetes Prevention Type 1) study that are available to qualified investigators through an application process. These samples are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. Information about how to apply for these materials can be obtained from the NIDDK Central Repositories by contacting Ms. Helen Ray of RTI, 1-919-316-3418, or hmp@rti.org Direct scientific-technical inquiry to the Project Officer of the NIDDK Central Repositories, Dr. Rebekah Rasooly, at phone: (301) 594-6007; e-mail: rr185i@nih.gov Visit the Repositories Web site at http://www.niddkrepository.org. NICHD - Brain and Tissue Bank for Developmental Disorders The purpose of the Bank is to collect, preserve, and distribute human tissues to investigators interested in autism and developmental disorders; normal tissues may be available for other research purposes. Further information can be obtained at www.btbank.org. The contact persons are H. Ron Zielke or Sally Wisniewsky, University of Maryland (1-800-847-1539), and Carol Petito or Stephanie Lojko, University of Miami (1-800-592-7246). NICHD - Reproductive Tissue Sample Repository (RTSaR) The Reproductive Tissue Sample Repository (RTSaR) is a virtual repository with online tissue sample acquisition capabilities. The RTSaR provides investigators with real-time access to human and nonhuman primate tissue and fluid inventories from four tissue bank facilities that are supported through the Specialized Cooperative Centers Program in Reproduction Research. The tissue banks are located at the University of California, San Diego (human ovary bank), Stanford University (human endometrium and DNA bank), Johns Hopkins University (male reproductive tissues and fluids), and the Oregon National Primate Research Center (nonhuman primate tissues). The web site for the RTSaR is https://rtsar.nichd.nih.gov/rtsar/login. If you wish to access the RTSaR, you can request an id and password to access the system by contacting the network administrator at RTSaR@mail.nih.gov Once you access the system, contact information for each bank is provided. Access is open to all investigators living in North America who are supported by research and research training grants from the NIH. One id and password will be provided to each principal investigator that can be utilized by any person working in the P.I.’s laboratory, or, in the case of institutional training grants (T32) and institutional career development award programs (K12), any person supported by the aforementioned awards. NCRR - Human Tissues and Organs Resource (HTOR) The Human Tissues and Organs Resource (HTOR) cooperative agreement supports a procurement network developed by the National Disease Research Interchange (NDRI), a not-for-profit organization. By collaborating with various medical centers, hospitals, pathology services, eye banks, tissue banks, and organ procurement organizations, HTOR provides a wide variety of human tissues and organs—both diseased and normal—to researchers for laboratory studies. Such samples include tissues from the central nervous system and brain, cardiovascular system, endocrine system, eyes, bone, and cartilage. For further information, consult the NDRI Web site (www.ndri.com) or contact Dr. John T. Lonsdale at NDRI, 8 Penn Center, 8th Floor, 1628 JFK Boulevard, Philadelphia, PA 19103. Phone: (800) 222-6374, ext. 271; fax: (215) 557-7154; e-mail: jlonsdale@ndriresource.org The NDRI Web site is http://www.ndri.com. NCRR - Islet Cell Resource (ICR) With support from NCRR, 10 Islet Cell Resource (ICR) centers isolate, purify, and characterize human pancreatic islets for subsequent transplantation into patients with type I diabetes. The ICR centers procure whole pancreata and acquire relevant data about donors; improve islet isolation and purification techniques; distribute islets for use in approved clinical protocols; and perfect the methods of storage and shipping. In this way, the centers optimize the viability, function, and availability of islets and help clinical researchers capitalize on the recently reported successes in islet transplantation. Information on submitting requests for islet cells can be obtained from Mr. John Kaddis, ICR Coordinating Center Project Manager, City of Hope National Medical Center, 1500 E. Duarte Road, Duarte, California 91010. Phone (626) 359-8111, ext. 63377; fax: (626) 471-7106; e-mail: jkaddis@coh.org The Coordinating Center hosts a Web site at http://icr.coh.org. NIA - SWAN Repository (longitudinal, multiethnic study of women at midlife including the menopausal transition) The SWAN Repository is a biologic specimen bank of the Study of Women’s Health Across the Nation (SWAN). The SWAN cohort was recruited in 1996/1997 and consists of 3302 African-American, Caucasian, Chinese, Hispanic, and Japanese women. The SWAN Repository contains more than 350,000 blood and urine specimens generated from the study participants’ annual visits (8 visits to date), at which time medical and health history, psychosocial measures, biological measures, and anthropometric data were and are being collected. In addition, a subset of the participants are providing urine samples, collected daily over the length of one menstrual cycle, each year. More than 900,000 of these samples are in the SWAN Repository and are available to researchers who wish to study the midlife and menopausal transition. Additionally, a DNA sample repository is also available and includes DNA as well as transformed B-lymphoblastoid cell lines from more than 1800 of the participants. To learn more about the SWAN Repository and how to apply to use SWAN Repository specimens, contact the Web site at http://www.swanrepository.com or Dr. MaryFran Sowers, University of Michigan, School of Public Health, Epidemiology Dept., (734) 936-3892; e-mail: mfsowers@umich.edu Human and Animal Cell and Biologic Reagent Resources NIDDK - National Hormone and Peptide Program The National Hormone and Peptide Program (NHPP) offers peptide hormones and their antisera, tissues (rat hypothalami), and miscellaneous reagents to qualified investigators. These reagents are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. These materials can be obtained from Dr. A. F. Parlow of the Harbor-UCLA Medical Center, Research and Education Institute, Torrance, CA. A more complete description of resources within this program is provided in The Endocrine Society journals. Direct scientific-technical inquiry to NHPP Scientific Director, Dr. Al Parlow, at phone: (310) 222-3537; fax: (310) 222-3432; e-mail: parlow@humc.edu Visit the NHPP Web site at http://www.humc.edu/hormones. NICHD - National Hormone and Pituitary Program (see NIDDK listing) Following is a list of reagents currently available through the resources of NICHD: Androgen receptor and peptide antigenRecombinant monkey (cynomolgus) and baboon luteinizing hormone and follicle-stimulating hormone and antisera. NIA - Aging Cell Bank To facilitate aging research on cells in culture, the NIA provides support for the Aging Cell Bank located at the Coriell Institute for Medical Research in Camden, NJ. The Aged Cell Bank provides fibroblast, lymphoblastoid, and differentiated cell lines from a wide range of human age-related conditions and other mammalian species, as well as DNA from a limited subset of cell lines. For further information, the Aged Cell Bank catalog can be accessed at http://locus.umdnj.edu/nia or contact Dr. Donald Coppock at 1-800-752-3805. NCRR - Various Cell Repositories NCRR maintains the following cell repository resources: National Cell Culture Center, National Stem Cell Resource, and the Yeast Genetic Stock Center. Further information regarding these resources may be obtained through the NCRR Web site at: www.ncrr.nih.gov/ncrrprog/cmpdir/BIOLOG.asp. Animal Resources NIA - Aging Rodent Resources NIA maintains both rat and mouse colonies for use by the scientific community. The animals available range in age from 1 to 36 months. A repository of fresh-frozen tissue from the NIA aged rodent colonies is stocked with tissue from mouse and rat strains, including caloric-restricted BALB/c mice. The NIA also maintains a colony of calorically restricted rodents of selected genotypes, which are available to the scientific community. For further information, please refer to the Aged Rodent information handbook at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentColoniesHandbook/ or contact the Office of Biological Resources and Resource Development order desk. Phone: (301) 496-0181; fax: (301) 402-5597; e-mail: rodents@nia.nih.gov NIA - Aged Rodent Tissue Bank The rodent tissue bank contains flash-frozen tissues from rodents in the NIA aged rodent colonies. Tissue is collected from rodents at 4 or 5 age points throughout the lifespan. Tissue arrays are also available. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentTissueBankHandbook/. NCRR - Mutant Mouse Regional Resource Centers (MMRRC) The Mutant Mouse Regional Resource Center (MMRRC) Program consists of centers that collectively operate as a one-stop shop to serve the biomedical research community. Investigators who have created select mutant mouse models may donate their models to an MMRRC for broad dissemination to other investigators who request them for noncommercial research investigations related to human health, disease, and treatments. The NCRR Division of Comparative Medicine (DCM) supports the MMRRCs, which are electronically linked through the MMRRC Informatics Coordinating Center (ICC) to function as one facility. The ICC, located at The Jackson Laboratory in Bar Harbor, ME, provides database and other informatics support to the MMRRC to give the research community a single entry point to the program. Further information can be obtained from the Web site at http://www.mmrrc.org, or from Franziska Grieder, D.V.M., Ph.D., Division of Comparative Medicine, NCRR. Phone (301) 435-0744; fax: (301) 480-3819; e-mail: griederf@ncrr.nih.gov NCRR - Induced Mutant Mouse Resource (IMR) The Induced Mutant Mouse Resource (IMR) at The Jackson Laboratory provides researchers with genetically engineered mice (transgenic, targeted mutant, retroviral insertional mutant, and chemically induced mutant mice). The function of the IMR is to select, import, cryopreserve, maintain, and distribute these important strains of mice to the research community. To improve their value for research, the IMR also undertakes genetic development of stocks, such as transferring mutant genes or transgenes to defined genetic backgrounds and combining transgenes and/or targeted mutations to create new mouse models for research. Over 800 mutant stocks have been accepted by the IMR. Current holdings include models for research on cancer, immunological and inflammatory diseases, neurological diseases and behavioral disorders, cardiovascular diseases, developmental disorders, metabolic and other diseases, reporter (e.g. GFP) and recombinase (e.g. cre/loxP) strains. About 8 strains a month are being added to the IMR holdings. A list of all strains may be obtained from the IMR Web site: www.jax.org/resources/documents/imr/. Online submission forms are also available on that site. All mice can be ordered by calling The Jackson Laboratory’s Customer Service Department at 1-800-422-MICE or (207) 288-5845 or by faxing (207) 288-6150. NIDDK - Mouse Metabolic Phenotyping Centers The mission of the Mouse Metabolic Phenotyping Centers is to provide the scientific community with standardized, high-quality metabolic and physiologic phenotyping services for mouse models of diabetes, diabetic complications, obesity, and related disorders. Researchers can ship mice to one of the four Centers (University of Cincinnati, University of Texas Southwestern Medical Center, Vanderbilt University, and Yale University) and obtain on a fee-for-service basis a range of complex exams used to characterize mouse metabolism, blood composition, energy balance, eating and exercise, organ function and morphology, physiology, and histology. Many tests are done in living animals and are designed to elucidate the subtle hallmarks of metabolic disease. Information, including a complete list of available tests, can be found at www.mmpc.org, or contact Dr. Maren R. Laughlin, NIDDK, at (301) 594-8802; e-mail: Maren.Laughlin@nih.gov NCRR - National Primate Research Centers (NPRCs) National Primate Research Centers (NPRCs) are a network of eight highly specialized facilities for nonhuman primates (NHP) research. Funded by grants through NCRR’s Division of Comparative Medicine (DCM), each center, staffed with experienced research and support staff, provides the appropriate research environment to foster the development of NHP models of human health and disease for biomedical investigations. The NPRCs are affiliated with academic institutions and are accessible to eligible biomedical and behavioral investigators supported by research project grants from the National Institutes of Health and other sources. Further information may be obtained from the notice, Procedures for Accessing Regional Primate Research Centers, published in the NIH Guide for Grants and Contracts at http://grants2.nih.gov/grants/guide/notice-files/not97-014.html, or from John Harding, Ph.D., National Primate Research Centers and AIDS Animal Models Program, Division of Comparative Medicine, NCRR. Phone: (301) 435-0744; fax: (301) 480-3819; e-mail: hardingj@mail.nih.gov NIA - Nonhuman Primates, Aging Set-Aside Colony NIA maintains approximately 200 nonhuman primates (M. mulatta) at four National Primate Research Centers (see above) for conducting research on aging. These animals range in age from 18 to 35 years. While these animals are predominantly reserved for non-invasive research, exceptions can be made to this policy. For further information, please contact Dr. Nancy Nadon, Office of Biological Resources and Resource Development, NIA. Phone: (301) 402-7744; fax: (301) 402-0010; e-mail: nadonn@nia.nih.gov NIA - Nonhuman Primate (NHP) Tissue Bank and Aging Database The NIA developed two new resources to facilitate research in the NHP model. The NHP tissue bank contains fresh-frozen and fixed tissue donated by primate centers around the country. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/NHPTissueBankHandbook.htm. The Primate Aging Database provides an internet accessible database with data from thousands of primates around the country. It can be used to investigate the effect of age on a variety of parameters, predominantly blood chemistry and husbandry measurements. The site is password protected. The URL is http://ipad.primate.wisc.edu. NIA - Obesity, Diabetes and Aging Animal Resource (USF-ODARC) The NIA supports a colony of aged rhesus macaques, many of which are obese and/or diabetic. This is a long-term colony of monkeys housed at the University of South Florida’s Obesity, Diabetes and Aging Research Center. They have been extensively and longitudinally characterized for general health variables, blood chemistry, food intake, and body weight. Diabetic monkeys are tested daily for urine glucose and ketone levels, and prediabetic monkeys are tested weekly. Data for some of the monkeys extend as far back as 15 years. This unique resource is available for collaborative studies. ODARC has a significant amount of stored tissue collected at necropsy and stored blood/plasma collected longitudinally. Serial blood collection or tissue collection at necropsy can also be performed prospectively. Testing and imaging can also be performed on the monkeys. Inquiries regarding collaborative studies using the ODARC colony should be directed to: Barbara C. Hansen, Ph.D., Director, Obesity, Diabetes and Aging Research Center, University of South Florida, All Children’s Hospital, 801 6th Street South #9340, St. Petersburg, FL 33701. Phone: (727) 767-6993; fax: (727) 767-7443; e-mail: bchansen@aol.com NCRR - Various Animal Resources NCRR maintains the following animal resources: Animal Models and Genetic Stocks, Chimpanzee Biomedical Research Program, NIH Animal Genetic Resource, and the Specific Pathogen Free Macaque Breeding and Research Program. Further information regarding these and other resources may be obtained through the NCRR Web site at www.ncrr.nih.gov/comparative_med.asp. In Silico Resources NIDDK, NHLBI, and NIEHS - Nuclear Receptor Signaling Atlas The Nuclear Receptor Signaling Atlas (NURSA) has created an in silico resource comprised of curated information about Nuclear Receptors, Coregulators, Ligands, and Downstream Targets. NURSA is sponsored by NIH and provides online access through a public webportal at www.NURSA.org. Ease of navigation through a series of molecule pages allows users to make queries about Nuclear Receptors, Coactivators and Corepressors. Additional information about nuclear receptor ligands is provided, as well as primary datasets relating to expression profiling of nuclear receptors, coregulators and downstream targets. The molecule pages are hyperlinked to data contained in external databases, including NCBI, KEGG, UniProt, and others, allowing for detailed data mining. In partnership with The Endocrine Society, NURSA and Molecular Endocrinology (http://mend.endojournals.org/) have reciprocal links designed to enhance publications in Molecular Endocrinology and the information available through the NURSA molecule pages. Links to additional relevant literature citations are from PubMed at the National Library of Medicine. Miscellaneous Resources NCRR - National Gene Vector Laboratories (NGVLs) The National Gene Vector Laboratories (NGVLs), with core funding from NCRR, serve as a resource for researchers to obtain adequate quantities of clinical-grade vectors for human gene transfer protocols. The vector types include retrovirus, lentivirus, adenovirus, adeno-associated virus, herpes-virus, and DNA plasmids. The NGVLs consist of three vector production centers at: Baylor College of Medicine; City of Hope National Medical Center and Beckman Research Institute; and Indiana University, which also serves as the Coordinating Center for all the laboratories. Two additional laboratories conduct toxicology studies for NGVL-approved investigators. These laboratories are located at the Southern Research Institute and the University of Florida. Additional information about the process for requesting vector production and/or pharmacology/toxicology support should be directed to Ms. Lorraine Matheson, NGVL Project Coordinator, Indiana University School of Medicine. Phone: (317) 274-4519; fax: (317) 278-4518; e-mail: lrubin@iupui.edu The NGVL Coordinating Center at Indiana University also hosts a Web site at http://www.ngvl.org. NCRR - General Clinical Research Centers (GCRCs) The General Clinical Research Centers (GCRCs) are a national network of 82 centers that provide optimal settings for medical investigators to conduct safe, controlled, state-of-the-art in-patient and out-patient studies of both children and adults. GCRCs also provide infrastructure and resources that support several career development opportunities. Investigators who have research project funding from the National Institutes of Health (NIH) and other peer-reviewed sources may apply to use GCRCs. Because the GCRCs support a full spectrum of patient-oriented scientific inquiry, researchers who use these centers can benefit from collaborative, multidisciplinary research opportunities. To request access to a GCRC facility, eligible investigators should initially contact a GCRC program director, listed in the National Center for Research Resources (NCRR) Clinical Research Resources Directory (www.ncrr.nih.gov/ncrrprog/clindir/crdirectory.asp). Further information can be obtained from Anthony R. Hayward, M.D., Director, Division for Clinical Research Resources, National Center for Research Resources at NIH. Phone: (301) 435-0790; e-mail: haywarda@ncrr.nih.gov

Resources currently available to the scientific community that may be of interest for endocrinology research are described briefly here. More information is available through The Endocrine Society Home Page (http://www.endo-society.org) or the information provided below. HUMAN TISSUE AND BIOLOGIC SPECIMEN RESOURCES NCI - Cooperative Human Tissue Network (CHTN) The NCI Cooperative Human Tissue Network (CHTN) provides normal, benign, precancerous, and cancerous human tissue to the scientific community for biomedical research. Specimens are collected according to the investigator’s individual protocol. Information provided with the specimens includes routine histopathologic and demographic data. The CHTN can also provide a variety of tissue microarrays. Contact the CHTN Web site at http://www-chtn.ims.nci.nih.gov, or 1-866-GO2-CHTN (1-866-462-2486). NCI - Cooperative Breast Cancer Tissue Resource (CBCTR) The NCI Cooperative Breast Cancer Tissue Resource (CBCTR) can provide researchers with access to formalin-fixed, paraffin-embedded primary breast cancer specimens, with associated pathologic, clinical, and outcome data. All specimens are evaluated for pathologic diagnosis by CBCTR pathologists using standard diagnostic criteria. The collection is particularly well suited for validation studies of diagnostic and prognostic markers. The CBCTR also makes available breast cancer tissue microarrays designed by NCI statisticians to provide high statistical power for studies of stage-specific markers of breast cancer. Contact CBCTR’s Web site at http://cbctr.nci.nih.gov, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com. NCI - Cooperative Prostate Cancer Tissue Resource (CPCTR) The NCI Cooperative Prostate Cancer Tissue Resource (CPCTR) can provide access to over 4,000 cases of formalin-fixed, paraffin-embedded primary prostate cancer specimens, with associated pathology and clinical data. Fresh-frozen tissue is also available with limited clinical follow-up information. In addition, slides from prostate cancer tissue microarrays with associated pathology and clinical data are now available. Contact the CPCTR Web site at http://www.prostatetissues.org, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com. NCI - AIDS and Cancer Specimen Resource (ACSR) The AIDS and Cancer Specimen Resource (ACSR) provides qualified researchers with tissue, cell, blood, and fluid specimens, as well as clinical data from patients with AIDS and cancer. The specimens and clinical data are available for research studies, particularly those that translate basic research findings to clinical application. Contact the ACSR Web site (http://acsr.ucsf.edu/) or Dr. Kishor Bhatia, (301) 496-7147; e-mail: bhatiak@mail.nih.gov. NCI - Breast and Ovarian Cancer Family Registries (CFRs) The Breast and Ovarian CFRs facilitate and support interdisciplinary and population-based research on the identification and characterization of breast and ovarian cancer susceptibility genes, with particular emphasis on gene-gene and gene-environment interaction research. Available from the registries are: a) family history, epidemiologic and clinical data, b) updates on cancer recurrence, morbidity and mortality in participating families, and c) biospecimens, including plasma, lymphocytes, serum, DNA, Guthrie cards or buccal smears, and paraffin blocks of tumor tissue. For further information on these registries, contact the CFR Web site (http://epi.grants.cancer.gov/BCFR) or (301) 496-9600. NCI - Specimen Resource Locator The NCI Specimen Resource Locator (http://cancer.gov/specimens) is a database that helps researchers locate specimens for research. The database includes resources such as tissue banks and tissue procurement systems with access to normal, benign, precancerous, and/or cancerous human tissue covering a wide variety of organ sites. Researchers specify the types of specimens, number of cases, preservation methods, and associated data they require. The Locator will search the database and return a list of tissue resources most likely to meet their requirements. When no match is obtained, the researcher is referred to the NCI Tissue Expediter [(301) 496-7147; e-mail: tissexp@mail.nih.gov]. The Tissue Expediter is a scientist who can help match researchers with appropriate resources or identify appropriate collaborators when those are necessary. NIDDK - Biologic Samples from Diabetic Study Foundation A portion (1/3) of all stored nonrenewable samples (plasma, serum, urine) from subjects enrolled in the Diabetes Control and Complications Trial (DCCT) is available for use by the scientific community to address questions for which these samples may be invaluable. Announcements for using this resource appear in the NIH Guide for Grants and Contracts periodically. Inquiries may be addressed to: Catherine C. Cowie, Ph.D., Director, Diabetes Epidemiology Program, NIDDK, 6707 Democracy Blvd., Room 691, MSC 5460, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892-5460. Phone: (301) 594-8804; fax: (301) 480-3503; e-mail: cowiec@extra.niddk.nih.gov. NIDDK - NIDDK Central Repositories (Diabetes Prevention Study) The NIDDK Central Repositories have selected biosamples from the DPT-1 (The Diabetes Prevention Type 1) study that are available to qualified investigators through an application process. These samples are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. Information about how to apply for these materials can be obtained from the NIDDK Central Repositories by contacting Ms. Helen Ray of RTI, 1-919-316-3418, or hmp@rti.org. Direct scientific-technical inquiry to the Project Officer of the NIDDK Central Repositories, Dr. Rebekah Rasooly, at phone: (301) 594-6007; e-mail: rr185i@nih.gov. Visit the Repositories Web site at http://www.niddkrepository.org. NICHD - Brain and Tissue Bank for Developmental Disorders The purpose of the Bank is to collect, preserve, and distribute human tissues to investigators interested in autism and developmental disorders; normal tissues may be available for other research purposes. Further information can be obtained at www.btbank.org. The contact persons are H. Ron Zielke or Sally Wisniewsky, University of Maryland (1-800-847-1539), and Carol Petito or Stephanie Lojko, University of Miami (1-800-592-7246). NICHD - Reproductive Tissue Sample Repository (RTSaR) The Reproductive Tissue Sample Repository (RTSaR) is a virtual repository with online tissue sample acquisition capabilities. The RTSaR provides investigators with real-time access to human and nonhuman primate tissue and fluid inventories from four tissue bank facilities that are supported through the Specialized Cooperative Centers Program in Reproduction Research. The tissue banks are located at the University of California, San Diego (human ovary bank), Stanford University (human endometrium and DNA bank), Johns Hopkins University (male reproductive tissues and fluids), and the Oregon National Primate Research Center (nonhuman primate tissues). The web site for the RTSaR is https://rtsar.nichd.nih.gov/rtsar/login. If you wish to access the RTSaR, you can request an id and password to access the system by contacting the network administrator at RTSaR@mail.nih.gov. Once you access the system, contact information for each bank is provided. Access is open to all investigators living in North America who are supported by research and research training grants from the NIH. One id and password will be provided to each principal investigator that can be utilized by any person working in the P.I.’s laboratory, or, in the case of institutional training grants (T32) and institutional career development award programs (K12), any person supported by the aforementioned awards. NCRR - Human Tissues and Organs Resource (HTOR) The Human Tissues and Organs Resource (HTOR) cooperative agreement supports a procurement network developed by the National Disease Research Interchange (NDRI), a not-for-profit organization. By collaborating with various medical centers, hospitals, pathology services, eye banks, tissue banks, and organ procurement organizations, HTOR provides a wide variety of human tissues and organs—both diseased and normal—to researchers for laboratory studies. Such samples include tissues from the central nervous system and brain, cardiovascular system, endocrine system, eyes, bone, and cartilage. For further information, consult the NDRI Web site (www.ndri.com) or contact Dr. John T. Lonsdale at NDRI, 8 Penn Center, 8th Floor, 1628 JFK Boulevard, Philadelphia, PA 19103. Phone: (800) 222-6374, ext. 271; fax: (215) 557-7154; e-mail: jlonsdale@ndriresource.org. The NDRI Web site is http://www.ndri.com. NCRR - Islet Cell Resource (ICR) With support from NCRR, 10 Islet Cell Resource (ICR) centers isolate, purify, and characterize human pancreatic islets for subsequent transplantation into patients with type I diabetes. The ICR centers procure whole pancreata and acquire relevant data about donors; improve islet isolation and purification techniques; distribute islets for use in approved clinical protocols; and perfect the methods of storage and shipping. In this way, the centers optimize the viability, function, and availability of islets and help clinical researchers capitalize on the recently reported successes in islet transplantation. Information on submitting requests for islet cells can be obtained from Mr. John Kaddis, ICR Coordinating Center Project Manager, City of Hope National Medical Center, 1500 E. Duarte Road, Duarte, California 91010. Phone (626) 359-8111, ext. 63377; fax: (626) 471-7106; e-mail: jkaddis@coh.org. The Coordinating Center hosts a Web site at http://icr.coh.org. NIA - SWAN Repository (longitudinal, multiethnic study of women at midlife including the menopausal transition) The SWAN Repository is a biologic specimen bank of the Study of Women’s Health Across the Nation (SWAN). The SWAN cohort was recruited in 1996/1997 and consists of 3302 African-American, Caucasian, Chinese, Hispanic, and Japanese women. The SWAN Repository contains more than 350,000 blood and urine specimens generated from the study participants’ annual visits (8 visits to date), at which time medical and health history, psychosocial measures, biological measures, and anthropometric data were and are being collected. In addition, a subset of the participants are providing urine samples, collected daily over the length of one menstrual cycle, each year. More than 900,000 of these samples are in the SWAN Repository and are available to researchers who wish to study the midlife and menopausal transition. Additionally, a DNA sample repository is also available and includes DNA as well as transformed B-lymphoblastoid cell lines from more than 1800 of the participants. To learn more about the SWAN Repository and how to apply to use SWAN Repository specimens, contact the Web site at http://www.swanrepository.com or Dr. MaryFran Sowers, University of Michigan, School of Public Health, Epidemiology Dept., (734) 936-3892; e-mail: mfsowers@umich.edu. HUMAN AND ANIMAL CELL AND BIOLOGIC REAGENT RESOURCES NIDDK - National Hormone and Peptide Program The National Hormone and Peptide Program (NHPP) offers peptide hormones and their antisera, tissues (rat hypothalami), and miscellaneous reagents to qualified investigators. These reagents are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. These materials can be obtained from Dr. A. F. Parlow of the Harbor-UCLA Medical Center, Research and Education Institute, Torrance, CA. A more complete description of resources within this program is provided in The Endocrine Society journals. Direct scientific-technical inquiry to NHPP Scientific Director, Dr. Al Parlow, at phone: (310) 222-3537; fax: (310) 222-3432; e-mail: parlow@humc.edu. Visit the NHPP Web site at http://www.humc.edu/hormones. NICHD - National Hormone and Pituitary Program (see NIDDK listing) Following is a list of reagents currently available through the resources of NICHD: Androgen receptor and peptide antigen Recombinant monkey (cynomolgus) and baboon luteinizing hormone and follicle-stimulating hormone and antisera. NIA - Aging Cell Bank To facilitate aging research on cells in culture, the NIA provides support for the Aging Cell Bank located at the Coriell Institute for Medical Research in Camden, NJ. The Aged Cell Bank provides fibroblast, lymphoblastoid, and differentiated cell lines from a wide range of human age-related conditions and other mammalian species, as well as DNA from a limited subset of cell lines. For further information, the Aged Cell Bank catalog can be accessed at http://locus.umdnj.edu/nia or contact Dr. Donald Coppock at 1-800-752-3805. NCRR - Various Cell Repositories NCRR maintains the following cell repository resources: National Cell Culture Center, National Stem Cell Resource, and the Yeast Genetic Stock Center. Further information regarding these resources may be obtained through the NCRR Web site at: www.ncrr.nih.gov/ncrrprog/cmpdir/BIOLOG.asp. ANIMAL RESOURCES NIA - Aging Rodent Resources NIA maintains both rat and mouse colonies for use by the scientific community. The animals available range in age from 1 to 36 months. A repository of fresh-frozen tissue from the NIA aged rodent colonies is stocked with tissue from mouse and rat strains, including caloric-restricted BALB/c mice. The NIA also maintains a colony of calorically restricted rodents of selected genotypes, which are available to the scientific community. For further information, please refer to the Aged Rodent information handbook at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentColoniesHandbook/ or contact the Office of Biological Resources and Resource Development order desk. Phone: (301) 496-0181; fax: (301) 402-5597; e-mail: rodents@nia.nih.gov. NIA - Aged Rodent Tissue Bank The rodent tissue bank contains flash-frozen tissues from rodents in the NIA aged rodent colonies. Tissue is collected from rodents at 4 or 5 age points throughout the lifespan. Tissue arrays are also available. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentTissueBankHandbook/. NCRR - Mutant Mouse Regional Resource Centers (MMRRC) The Mutant Mouse Regional Resource Center (MMRRC) Program consists of centers that collectively operate as a one-stop shop to serve the biomedical research community. Investigators who have created select mutant mouse models may donate their models to an MMRRC for broad dissemination to other investigators who request them for noncommercial research investigations related to human health, disease, and treatments. The NCRR Division of Comparative Medicine (DCM) supports the MMRRCs, which are electronically linked through the MMRRC Informatics Coordinating Center (ICC) to function as one facility. The ICC, located at The Jackson Laboratory in Bar Harbor, ME, provides database and other informatics support to the MMRRC to give the research community a single entry point to the program. Further information can be obtained from the Web site at http://www.mmrrc.org, or from Franziska Grieder, D.V.M., Ph.D., Division of Comparative Medicine, NCRR. Phone (301) 435-0744; fax: (301) 480-3819; e-mail: griederf@ncrr.nih.gov. NCRR - Induced Mutant Mouse Resource (IMR) The Induced Mutant Mouse Resource (IMR) at The Jackson Laboratory provides researchers with genetically engineered mice (transgenic, targeted mutant, retroviral insertional mutant, and chemically induced mutant mice). The function of the IMR is to select, import, cryopreserve, maintain, and distribute these important strains of mice to the research community. To improve their value for research, the IMR also undertakes genetic development of stocks, such as transferring mutant genes or transgenes to defined genetic backgrounds and combining transgenes and/or targeted mutations to create new mouse models for research. Over 800 mutant stocks have been accepted by the IMR. Current holdings include models for research on cancer, immunological and inflammatory diseases, neurological diseases and behavioral disorders, cardiovascular diseases, developmental disorders, metabolic and other diseases, reporter (e.g. GFP) and recombinase (e.g. cre/loxP) strains. About 8 strains a month are being added to the IMR holdings. A list of all strains may be obtained from the IMR Web site: www.jax.org/resources/documents/imr/. Online submission forms are also available on that site. All mice can be ordered by calling The Jackson Laboratory’s Customer Service Department at 1-800-422-MICE or (207) 288-5845 or by faxing (207) 288-6150. NIDDK - Mouse Metabolic Phenotyping Centers The mission of the Mouse Metabolic Phenotyping Centers is to provide the scientific community with standardized, high-quality metabolic and physiologic phenotyping services for mouse models of diabetes, diabetic complications, obesity, and related disorders. Researchers can ship mice to one of the four Centers (University of Cincinnati, University of Texas Southwestern Medical Center, Vanderbilt University, and Yale University) and obtain on a fee-for-service basis a range of complex exams used to characterize mouse metabolism, blood composition, energy balance, eating and exercise, organ function and morphology, physiology, and histology. Many tests are done in living animals and are designed to elucidate the subtle hallmarks of metabolic disease. Information, including a complete list of available tests, can be found at www.mmpc.org, or contact Dr. Maren R. Laughlin, NIDDK, at (301) 594-8802; e-mail: Maren.Laughlin@nih.gov; or Dr. Kristin Abraham, NIDDK, at (301) 451-8048; e-mail: abrahamk@extra.niddk.nih.gov. NCRR - National Primate Research Centers (NPRCs) National Primate Research Centers (NPRCs) are a network of eight highly specialized facilities for nonhuman primates (NHP) research. Funded by grants through NCRR’s Division of Comparative Medicine (DCM), each center, staffed with experienced research and support staff, provides the appropriate research environment to foster the development of NHP models of human health and disease for biomedical investigations. The NPRCs are affiliated with academic institutions and are accessible to eligible biomedical and behavioral investigators supported by research project grants from the National Institutes of Health and other sources. Further information may be obtained from the notice, Procedures for Accessing Regional Primate Research Centers, published in the NIH Guide for Grants and Contracts at http://grants2.nih.gov/grants/guide/notice-files/not97-014.htm, or from John Harding, Ph.D., National Primate Research Centers and AIDS Animal Models Program, Division of Comparative Medicine, NCRR. Phone: (301) 435-0744; fax: (301) 480-3819; e-mail: hardingj@mail.nih.gov. NIA - Nonhuman Primates, Aging Set-Aside Colony NIA maintains approximately 200 nonhuman primates (M. mulatta) at four National Primate Research Centers (see above) for conducting research on aging. These animals range in age from 18 to 35 years. While these animals are predominantly reserved for non-invasive research, exceptions can be made to this policy. For further information, please contact Dr. Nancy Nadon, Office of Biological Resources and Resource Development, NIA. Phone: (301) 402-7744; fax: (301) 402-0010; e-mail: nadonn@nia.nih.gov. NIA - Nonhuman Primate (NHP) Tissue Bank and Aging Database The NIA developed two new resources to facilitate research in the NHP model. The NHP tissue bank contains fresh-frozen and fixed tissue donated by primate centers around the country. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/NHPTissueBankHandbook.htm. The Primate Aging Database provides an internet accessible database with data from thousands of primates around the country. It can be used to investigate the effect of age on a variety of parameters, predominantly blood chemistry and husbandry measurements. The site is password protected. The URL is http://ipad.primate.wisc.edu. NIA - Obesity, Diabetes and Aging Animal Resource (USF-ODARC) The NIA supports a colony of aged rhesus macaques, many of which are obese and/or diabetic. This is a long-term colony of monkeys housed at the University of South Florida’s Obesity, Diabetes and Aging Research Center. They have been extensively and longitudinally characterized for general health variables, blood chemistry, food intake, and body weight. Diabetic monkeys are tested daily for urine glucose and ketone levels, and prediabetic monkeys are tested weekly. Data for some of the monkeys extend as far back as 15 years. This unique resource is available for collaborative studies. ODARC has a significant amount of stored tissue collected at necropsy and stored blood/plasma collected longitudinally. Serial blood collection or tissue collection at necropsy can also be performed prospectively. Testing and imaging can also be performed on the monkeys. Inquiries regarding collaborative studies using the ODARC colony should be directed to: Barbara C. Hansen, Ph.D., Director, Obesity, Diabetes and Aging Research Center, University of South Florida, All Children’s Hospital, 801 6th Street South #9340, St. Petersburg, FL 33701. Phone: (727) 767-6993; fax: (727) 767-7443; e-mail: bchansen@aol.com. NCRR - Various Animal Resources NCRR maintains the following animal resources: Animal Models and Genetic Stocks, Chimpanzee Biomedical Research Program, NIH Animal Genetic Resource, and the Specific Pathogen Free Macaque Breeding and Research Program. Further information regarding these and other resources may be obtained through the NCRR Web site at www.ncrr.nih.gov/comparative_med.asp. IN SILICO RESOURCES NIDDK, NHLBI, and NIEHS - Nuclear Receptor Signaling Atlas The Nuclear Receptor Signaling Atlas (NURSA) has created an in silico resource comprised of curated information about Nuclear Receptors, Coregulators, Ligands, and Downstream Targets. NURSA is sponsored by NIH and provides online access through a public webportal at www.NURSA.org. Ease of navigation through a series of molecule pages allows users to make queries about Nuclear Receptors, Coactivators and Corepressors. Additional information about nuclear receptor ligands is provided, as well as primary datasets relating to expression profiling of nuclear receptors, coregulators and downstream targets. The molecule pages are hyperlinked to data contained in external databases, including NCBI, KEGG, UniProt, and others, allowing for detailed data mining. In partnership with The Endocrine Society, NURSA and Molecular Endocrinology (http://mend.endojournals.org/) have reciprocal links designed to enhance publications in Molecular Endocrinology and the information available through the NURSA molecule pages. Links to additional relevant literature citations are from PubMed at the National Library of Medicine. MISCELLANEOUS RESOURCES NCRR - National Gene Vector Laboratories (NGVLs) The National Gene Vector Laboratories (NGVLs), with core funding from NCRR, serve as a resource for researchers to obtain adequate quantities of clinical-grade vectors for human gene transfer protocols. The vector types include retrovirus, lentivirus, adenovirus, adeno-associated virus, herpes-virus, and DNA plasmids. The NGVLs consist of three vector production centers at: Baylor College of Medicine; City of Hope National Medical Center and Beckman Research Institute; and Indiana University, which also serves as the Coordinating Center for all the laboratories. Two additional laboratories conduct toxicology studies for NGVL-approved investigators. These laboratories are located at the Southern Research Institute and the University of Florida. Additional information about the process for requesting vector production and/or pharmacology/toxicology support should be directed to Ms. Lorraine Matheson, NGVL Project Coordinator, Indiana University School of Medicine. Phone: (317) 274-4519; fax: (317) 278-4518; e-mail: lrubin@iupui.edu. The NGVL Coordinating Center at Indiana University also hosts a Web site at http://www.ngvl.org. NCRR - General Clinical Research Centers (GCRCs) The General Clinical Research Centers (GCRCs) are a national network of 82 centers that provide optimal settings for medical investigators to conduct safe, controlled, state-of-the-art in-patient and out-patient studies of both children and adults. GCRCs also provide infrastructure and resources that support several career development opportunities. Investigators who have research project funding from the National Institutes of Health (NIH) and other peer-reviewed sources may apply to use GCRCs. Because the GCRCs support a full spectrum of patient-oriented scientific inquiry, researchers who use these centers can benefit from collaborative, multidisciplinary research opportunities. To request access to a GCRC facility, eligible investigators should initially contact a GCRC program director, listed in the National Center for Research Resources (NCRR) Clinical Research Resources Directory (www.ncrr.nih.gov/ncrrprog/clindir/crdirectory.asp). Further information can be obtained from Anthony R. Hayward, M.D., Director, Division for Clinical Research Resources, National Center for Research Resources at NIH. Phone: (301) 435-0790; e-mail: haywarda@ncrr.nih.gov.

Resources currently available to the scientific community that may be of interest for endocrinology research are described briefly here. More information is available through The Endocrine Society Home Page (http://www.endo-society.org) or the information provided below. HUMAN TISSUE AND BIOLOGIC SPECIMEN RESOURCES NCI - Cooperative Human Tissue Network (CHTN) The NCI Cooperative Human Tissue Network (CHTN) provides normal, benign, precancerous, and cancerous human tissue to the scientific community for biomedical research. Specimens are collected according to the investigator’s individual protocol. Information provided with the specimens includes routine histopathologic and demographic data. The CHTN can also provide a variety of tissue microarrays. Contact the CHTN Web site at http://www-chtn.ims.nci.nih.gov, or 1-866-GO2-CHTN (1-866-462-2486). NCI - Cooperative Breast Cancer Tissue Resource (CBCTR) The NCI Cooperative Breast Cancer Tissue Resource (CBCTR) can provide researchers with access to formalin-fixed, paraffin-embedded primary breast cancer specimens, with associated pathologic, clinical, and outcome data. All specimens are evaluated for pathologic diagnosis by CBCTR pathologists using standard diagnostic criteria. The collection is particularly well suited for validation studies of diagnostic and prognostic markers. The CBCTR also makes available breast cancer tissue microarrays designed by NCI statisticians to provide high statistical power for studies of stage-specific markers of breast cancer. Contact CBCTR’s Web site at http://cbctr.nci.nih.gov, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com. NCI - Cooperative Prostate Cancer Tissue Resource (CPCTR) The NCI Cooperative Prostate Cancer Tissue Resource (CPCTR) can provide access to over 4,000 cases of formalin-fixed, paraffin-embedded primary prostate cancer specimens, with associated pathology and clinical data. Fresh-frozen tissue is also available with limited clinical follow-up information. In addition, slides from prostate cancer tissue microarrays with associated pathology and clinical data are now available. Contact the CPCTR Web site at http://www.prostatetissues.org, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com. NCI - AIDS and Cancer Specimen Resource (ACSR) The AIDS and Cancer Specimen Resource (ACSR) provides qualified researchers with tissue, cell, blood, and fluid specimens, as well as clinical data from patients with AIDS and cancer. The specimens and clinical data are available for research studies, particularly those that translate basic research findings to clinical application. Contact the ACSR Web site (http://acsr.ucsf.edu/) or Dr. Kishor Bhatia, (301) 496-7147; e-mail: bhatiak@mail.nih.gov. NCI - Breast and Ovarian Cancer Family Registries (CFRs) The Breast and Ovarian CFRs facilitate and support interdisciplinary and population-based research on the identification and characterization of breast and ovarian cancer susceptibility genes, with particular emphasis on gene-gene and gene-environment interaction research. Available from the registries are: a) family history, epidemiologic and clinical data, b) updates on cancer recurrence, morbidity and mortality in participating families, and c) biospecimens, including plasma, lymphocytes, serum, DNA, Guthrie cards or buccal smears, and paraffin blocks of tumor tissue. For further information on these registries, contact the CFR Web site (http://epi.grants.cancer.gov/BCFR) or (301) 496-9600. NCI - Specimen Resource Locator The NCI Specimen Resource Locator (http://cancer.gov/specimens) is a database that helps researchers locate specimens for research. The database includes resources such as tissue banks and tissue procurement systems with access to normal, benign, precancerous, and/or cancerous human tissue covering a wide variety of organ sites. Researchers specify the types of specimens, number of cases, preservation methods, and associated data they require. The Locator will search the database and return a list of tissue resources most likely to meet their requirements. When no match is obtained, the researcher is referred to the NCI Tissue Expediter [(301) 496-7147; e-mail: tissexp@mail.nih.gov]. The Tissue Expediter is a scientist who can help match researchers with appropriate resources or identify appropriate collaborators when those are necessary. NIDDK - Biologic Samples from Diabetic Study Foundation A portion (1/3) of all stored nonrenewable samples (plasma, serum, urine) from subjects enrolled in the Diabetes Control and Complications Trial (DCCT) is available for use by the scientific community to address questions for which these samples may be invaluable. Announcements for using this resource appear in the NIH Guide for Grants and Contracts periodically. Inquiries may be addressed to: Catherine C. Cowie, Ph.D., Director, Diabetes Epidemiology Program, NIDDK, 6707 Democracy Blvd., Room 691, MSC 5460, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892-5460. Phone: (301) 594-8804; fax: (301) 480-3503; e-mail: cowiec@extra.niddk.nih.gov. NIDDK - NIDDK Central Repositories (Diabetes Prevention Study) The NIDDK Central Repositories have selected biosamples from the DPT-1 (The Diabetes Prevention Type 1) study that are available to qualified investigators through an application process. These samples are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. Information about how to apply for these materials can be obtained from the NIDDK Central Repositories by contacting Ms. Helen Ray of RTI, 1-919-316-3418, or hmp@rti.org. Direct scientific-technical inquiry to the Project Officer of the NIDDK Central Repositories, Dr. Rebekah Rasooly, at phone: (301) 594-6007; e-mail: rr185i@nih.gov. Visit the Repositories Web site at http://www.niddkrepository.org. NICHD - Brain and Tissue Bank for Developmental Disorders The purpose of the Bank is to collect, preserve, and distribute human tissues to investigators interested in autism and developmental disorders; normal tissues may be available for other research purposes. Further information can be obtained at www.btbank.org. The contact persons are H. Ron Zielke or Sally Wisniewsky, University of Maryland (1-800-847-1539), and Carol Petito or Stephanie Lojko, University of Miami (1-800-592-7246). NICHD - Reproductive Tissue Sample Repository (RTSaR) The Reproductive Tissue Sample Repository (RTSaR) is a virtual repository with online tissue sample acquisition capabilities. The RTSaR provides investigators with real-time access to human and nonhuman primate tissue and fluid inventories from four tissue bank facilities that are supported through the Specialized Cooperative Centers Program in Reproduction Research. The tissue banks are located at the University of California, San Diego (human ovary bank), Stanford University (human endometrium and DNA bank), Johns Hopkins University (male reproductive tissues and fluids), and the Oregon National Primate Research Center (nonhuman primate tissues). The web site for the RTSaR is https://rtsar.nichd.nih.gov/rtsar/login. If you wish to access the RTSaR, you can request an id and password to access the system by contacting the network administrator at RTSaR@mail.nih.gov. Once you access the system, contact information for each bank is provided. Access is open to all investigators living in North America who are supported by research and research training grants from the NIH. One id and password will be provided to each principal investigator that can be utilized by any person working in the P.I.’s laboratory, or, in the case of institutional training grants (T32) and institutional career development award programs (K12), any person supported by the aforementioned awards. NCRR - Human Tissues and Organs Resource (HTOR) The Human Tissues and Organs Resource (HTOR) cooperative agreement supports a procurement network developed by the National Disease Research Interchange (NDRI), a not-for-profit organization. By collaborating with various medical centers, hospitals, pathology services, eye banks, tissue banks, and organ procurement organizations, HTOR provides a wide variety of human tissues and organs—both diseased and normal—to researchers for laboratory studies. Such samples include tissues from the central nervous system and brain, cardiovascular system, endocrine system, eyes, bone, and cartilage. For further information, consult the NDRI Web site (www.ndri.com) or contact Dr. John T. Lonsdale at NDRI, 8 Penn Center, 8th Floor, 1628 JFK Boulevard, Philadelphia, PA 19103. Phone: (800) 222-6374, ext. 271; fax: (215) 557-7154; e-mail: jlonsdale@ndriresource.org. The NDRI Web site is http://www.ndri.com. NCRR - Islet Cell Resource (ICR) With support from NCRR, 10 Islet Cell Resource (ICR) centers isolate, purify, and characterize human pancreatic islets for subsequent transplantation into patients with type I diabetes. The ICR centers procure whole pancreata and acquire relevant data about donors; improve islet isolation and purification techniques; distribute islets for use in approved clinical protocols; and perfect the methods of storage and shipping. In this way, the centers optimize the viability, function, and availability of islets and help clinical researchers capitalize on the recently reported successes in islet transplantation. Information on submitting requests for islet cells can be obtained from Mr. John Kaddis, ICR Coordinating Center Project Manager, City of Hope National Medical Center, 1500 E. Duarte Road, Duarte, California 91010. Phone (626) 359-8111, ext. 63377; fax: (626) 471-7106; e-mail: jkaddis@coh.org. The Coordinating Center hosts a Web site at http://icr.coh.org. NIA - SWAN Repository (longitudinal, multiethnic study of women at midlife including the menopausal transition) The SWAN Repository is a biologic specimen bank of the Study of Women’s Health Across the Nation (SWAN). The SWAN cohort was recruited in 1996/1997 and consists of 3302 African-American, Caucasian, Chinese, Hispanic, and Japanese women. The SWAN Repository contains more than 350,000 blood and urine specimens generated from the study participants’ annual visits (8 visits to date), at which time medical and health history, psychosocial measures, biological measures, and anthropometric data were and are being collected. In addition, a subset of the participants are providing urine samples, collected daily over the length of one menstrual cycle, each year. More than 900,000 of these samples are in the SWAN Repository and are available to researchers who wish to study the midlife and menopausal transition. Additionally, a DNA sample repository is also available and includes DNA as well as transformed B-lymphoblastoid cell lines from more than 1800 of the participants. To learn more about the SWAN Repository and how to apply to use SWAN Repository specimens, contact the Web site at http://www.swanrepository.com or Dr. MaryFran Sowers, University of Michigan, School of Public Health, Epidemiology Dept., (734) 936-3892; e-mail: mfsowers@umich.edu. HUMAN AND ANIMAL CELL AND BIOLOGIC REAGENT RESOURCES NIDDK - National Hormone and Peptide Program The National Hormone and Peptide Program (NHPP) offers peptide hormones and their antisera, tissues (rat hypothalami), and miscellaneous reagents to qualified investigators. These reagents are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. These materials can be obtained from Dr. A. F. Parlow of the Harbor-UCLA Medical Center, Research and Education Institute, Torrance, CA. A more complete description of resources within this program is provided in The Endocrine Society journals. Direct scientific-technical inquiry to NHPP Scientific Director, Dr. Al Parlow, at phone: (310) 222-3537; fax: (310) 222-3432; e-mail: parlow@humc.edu. Visit the NHPP Web site at http://www.humc.edu/hormones. NICHD - National Hormone and Pituitary Program (see NIDDK listing) Following is a list of reagents currently available through the resources of NICHD: Androgen receptor and peptide antigen Recombinant monkey (cynomolgus) and baboon luteinizing hormone and follicle-stimulating hormone and antisera. NIA - Aging Cell Bank To facilitate aging research on cells in culture, the NIA provides support for the Aging Cell Bank located at the Coriell Institute for Medical Research in Camden, NJ. The Aged Cell Bank provides fibroblast, lymphoblastoid, and differentiated cell lines from a wide range of human age-related conditions and other mammalian species, as well as DNA from a limited subset of cell lines. For further information, the Aged Cell Bank catalog can be accessed at http://locus.umdnj.edu/nia or contact Dr. Donald Coppock at 1-800-752-3805. NCRR - Various Cell Repositories NCRR maintains the following cell repository resources: National Cell Culture Center, National Stem Cell Resource, and the Yeast Genetic Stock Center. Further information regarding these resources may be obtained through the NCRR Web site at: www.ncrr.nih.gov/ncrrprog/cmpdir/BIOLOG.asp. ANIMAL RESOURCES NIA - Aging Rodent Resources NIA maintains both rat and mouse colonies for use by the scientific community. The animals available range in age from 1 to 36 months. A repository of fresh-frozen tissue from the NIA aged rodent colonies is stocked with tissue from mouse and rat strains, including caloric-restricted BALB/c mice. The NIA also maintains a colony of calorically restricted rodents of selected genotypes, which are available to the scientific community. For further information, please refer to the Aged Rodent information handbook at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentColoniesHandbook/ or contact the Office of Biological Resources and Resource Development order desk. Phone: (301) 496-0181; fax: (301) 402-5597; e-mail: rodents@nia.nih.gov. NIA - Aged Rodent Tissue Bank The rodent tissue bank contains flash-frozen tissues from rodents in the NIA aged rodent colonies. Tissue is collected from rodents at 4 or 5 age points throughout the lifespan. Tissue arrays are also available. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentTissueBankHandbook/. NCRR - Mutant Mouse Regional Resource Centers (MMRRC) The Mutant Mouse Regional Resource Center (MMRRC) Program consists of centers that collectively operate as a one-stop shop to serve the biomedical research community. Investigators who have created select mutant mouse models may donate their models to an MMRRC for broad dissemination to other investigators who request them for noncommercial research investigations related to human health, disease, and treatments. The NCRR Division of Comparative Medicine (DCM) supports the MMRRCs, which are electronically linked through the MMRRC Informatics Coordinating Center (ICC) to function as one facility. The ICC, located at The Jackson Laboratory in Bar Harbor, ME, provides database and other informatics support to the MMRRC to give the research community a single entry point to the program. Further information can be obtained from the Web site at http://www.mmrrc.org, or from Franziska Grieder, D.V.M., Ph.D., Division of Comparative Medicine, NCRR. Phone (301) 435-0744; fax: (301) 480-3819; e-mail: griederf@ncrr.nih.gov. NCRR - Induced Mutant Mouse Resource (IMR) The Induced Mutant Mouse Resource (IMR) at The Jackson Laboratory provides researchers with genetically engineered mice (transgenic, targeted mutant, retroviral insertional mutant, and chemically induced mutant mice). The function of the IMR is to select, import, cryopreserve, maintain, and distribute these important strains of mice to the research community. To improve their value for research, the IMR also undertakes genetic development of stocks, such as transferring mutant genes or transgenes to defined genetic backgrounds and combining transgenes and/or targeted mutations to create new mouse models for research. Over 800 mutant stocks have been accepted by the IMR. Current holdings include models for research on cancer, immunological and inflammatory diseases, neurological diseases and behavioral disorders, cardiovascular diseases, developmental disorders, metabolic and other diseases, reporter (e.g. GFP) and recombinase (e.g. cre/loxP) strains. About 8 strains a month are being added to the IMR holdings. A list of all strains may be obtained from the IMR Web site: www.jax.org/resources/documents/imr/. Online submission forms are also available on that site. All mice can be ordered by calling The Jackson Laboratory’s Customer Service Department at 1-800-422-MICE or (207) 288-5845 or by faxing (207) 288-6150. NIDDK - Mouse Metabolic Phenotyping Centers The mission of the Mouse Metabolic Phenotyping Centers is to provide the scientific community with standardized, high-quality metabolic and physiologic phenotyping services for mouse models of diabetes, diabetic complications, obesity, and related disorders. Researchers can ship mice to one of the four Centers (University of Cincinnati, University of Texas Southwestern Medical Center, Vanderbilt University, and Yale University) and obtain on a fee-for-service basis a range of complex exams used to characterize mouse metabolism, blood composition, energy balance, eating and exercise, organ function and morphology, physiology, and histology. Many tests are done in living animals and are designed to elucidate the subtle hallmarks of metabolic disease. Information, including a complete list of available tests, can be found at www.mmpc.org, or contact Dr. Maren R. Laughlin, NIDDK, at (301) 594-8802; e-mail: Maren.Laughlin@nih.gov; or Dr. Kristin Abraham, NIDDK, at (301) 451-8048; e-mail: abrahamk@extra.niddk.nih.gov. NCRR - National Primate Research Centers (NPRCs) National Primate Research Centers (NPRCs) are a network of eight highly specialized facilities for nonhuman primates (NHP) research. Funded by grants through NCRR’s Division of Comparative Medicine (DCM), each center, staffed with experienced research and support staff, provides the appropriate research environment to foster the development of NHP models of human health and disease for biomedical investigations. The NPRCs are affiliated with academic institutions and are accessible to eligible biomedical and behavioral investigators supported by research project grants from the National Institutes of Health and other sources. Further information may be obtained from the notice, Procedures for Accessing Regional Primate Research Centers, published in the NIH Guide for Grants and Contracts at http://grants2.nih.gov/grants/guide/notice-files/not97-014.html, or from John Harding, Ph.D., National Primate Research Centers and AIDS Animal Models Program, Division of Comparative Medicine, NCRR. Phone: (301) 435-0744; fax: (301) 480-3819; e-mail: hardingj@mail.nih.gov. NIA - Nonhuman Primates, Aging Set-Aside Colony NIA maintains approximately 200 nonhuman primates (M. mulatta) at four National Primate Research Centers (see above) for conducting research on aging. These animals range in age from 18 to 35 years. While these animals are predominantly reserved for non-invasive research, exceptions can be made to this policy. For further information, please contact Dr. Nancy Nadon, Office of Biological Resources and Resource Development, NIA. Phone: (301) 402-7744; fax: (301) 402-0010; e-mail: nadonn@nia.nih.gov. NIA - Nonhuman Primate (NHP) Tissue Bank and Aging Database The NIA developed two new resources to facilitate research in the NHP model. The NHP tissue bank contains fresh-frozen and fixed tissue donated by primate centers around the country. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/NHPTissueBankHandbook.htm. The Primate Aging Database provides an internet accessible database with data from thousands of primates around the country. It can be used to investigate the effect of age on a variety of parameters, predominantly blood chemistry and husbandry measurements. The site is password protected. The URL is http://ipad.primate.wisc.edu. NIA - Obesity, Diabetes and Aging Animal Resource (USF-ODARC) The NIA supports a colony of aged rhesus macaques, many of which are obese and/or diabetic. This is a long-term colony of monkeys housed at the University of South Florida’s Obesity, Diabetes and Aging Research Center. They have been extensively and longitudinally characterized for general health variables, blood chemistry, food intake, and body weight. Diabetic monkeys are tested daily for urine glucose and ketone levels, and prediabetic monkeys are tested weekly. Data for some of the monkeys extend as far back as 15 years. This unique resource is available for collaborative studies. ODARC has a significant amount of stored tissue collected at necropsy and stored blood/plasma collected longitudinally. Serial blood collection or tissue collection at necropsy can also be performed prospectively. Testing and imaging can also be performed on the monkeys. Inquiries regarding collaborative studies using the ODARC colony should be directed to: Barbara C. Hansen, Ph.D., Director, Obesity, Diabetes and Aging Research Center, University of South Florida, All Children’s Hospital, 801 6th Street South #9340, St. Petersburg, FL 33701. Phone: (727) 767-6993; fax: (727) 767-7443; e-mail: bchansen@aol.com. NCRR - Various Animal Resources NCRR maintains the following animal resources: Animal Models and Genetic Stocks, Chimpanzee Biomedical Research Program, NIH Animal Genetic Resource, and the Specific Pathogen Free Macaque Breeding and Research Program. Further information regarding these and other resources may be obtained through the NCRR Web site at www.ncrr.nih.gov/comparative_med.asp. IN SILICO RESOURCES NIDDK, NHLBI, and NIEHS - Nuclear Receptor Signaling Atlas The Nuclear Receptor Signaling Atlas (NURSA) has created an in silico resource comprised of curated information about Nuclear Receptors, Coregulators, Ligands, and Downstream Targets. NURSA is sponsored by NIH and provides online access through a public webportal at www.NURSA.org. Ease of navigation through a series of molecule pages allows users to make queries about Nuclear Receptors, Coactivators and Corepressors. Additional information about nuclear receptor ligands is provided, as well as primary datasets relating to expression profiling of nuclear receptors, coregulators and downstream targets. The molecule pages are hyperlinked to data contained in external databases, including NCBI, KEGG, UniProt, and others, allowing for detailed data mining. In partnership with The Endocrine Society, NURSA and Molecular Endocrinology (http://mend.endojournals.org/) have reciprocal links designed to enhance publications in Molecular Endocrinology and the information available through the NURSA molecule pages. Links to additional relevant literature citations are from PubMed at the National Library of Medicine. MISCELLANEOUS RESOURCES NCRR - National Gene Vector Laboratories (NGVLs) The National Gene Vector Laboratories (NGVLs), with core funding from NCRR, serve as a resource for researchers to obtain adequate quantities of clinical-grade vectors for human gene transfer protocols. The vector types include retrovirus, lentivirus, adenovirus, adeno-associated virus, herpes-virus, and DNA plasmids. The NGVLs consist of three vector production centers at: Baylor College of Medicine; City of Hope National Medical Center and Beckman Research Institute; and Indiana University, which also serves as the Coordinating Center for all the laboratories. Two additional laboratories conduct toxicology studies for NGVL-approved investigators. These laboratories are located at the Southern Research Institute and the University of Florida. Additional information about the process for requesting vector production and/or pharmacology/toxicology support should be directed to Ms. Lorraine Matheson, NGVL Project Coordinator, Indiana University School of Medicine. Phone: (317) 274-4519; fax: (317) 278-4518; e-mail: lrubin@iupui.edu. The NGVL Coordinating Center at Indiana University also hosts a Web site at http://www.ngvl.org. NCRR - General Clinical Research Centers (GCRCs) The General Clinical Research Centers (GCRCs) are a national network of 82 centers that provide optimal settings for medical investigators to conduct safe, controlled, state-of-the-art in-patient and out-patient studies of both children and adults. GCRCs also provide infrastructure and resources that support several career development opportunities. Investigators who have research project funding from the National Institutes of Health (NIH) and other peer-reviewed sources may apply to use GCRCs. Because the GCRCs support a full spectrum of patient-oriented scientific inquiry, researchers who use these centers can benefit from collaborative, multidisciplinary research opportunities. To request access to a GCRC facility, eligible investigators should initially contact a GCRC program director, listed in the National Center for Research Resources (NCRR) Clinical Research Resources Directory (www.ncrr.nih.gov/ncrrprog/clindir/crdirectory.asp). Further information can be obtained from Anthony R. Hayward, M.D., Director, Division for Clinical Research Resources, National Center for Research Resources at NIH. Phone: (301) 435-0790; e-mail: haywarda@ncrr.nih.gov.

Abstract Resources currently available to the scientific community that may be of interest for endocrinology research are described briefly here. More information is available through The Endocrine Society Home Page (http://www.endo-society.org) or the information provided below. HUMAN TISSUE AND BIOLOGIC SPECIMEN RESOURCES NCI - Cooperative Human Tissue Network (CHTN) The NCI Cooperative Human Tissue Network (CHTN) provides normal, benign, precancerous, and cancerous human tissue to the scientific community for biomedical research. Specimens are collected according to the investigator’s individual protocol. Information provided with the specimens includes routine histopathologic and demographic data. The CHTN can also provide a variety of tissue microarrays. Contact the CHTN Web site at http://www-chtn.ims.nci.nih.gov, or 1-866-GO2-CHTN (1-866-462-2486). NCI - Cooperative Breast Cancer Tissue Resource (CBCTR) The NCI Cooperative Breast Cancer Tissue Resource (CBCTR) can provide researchers with access to formalin-fixed, paraffin-embedded primary breast cancer specimens, with associated pathologic, clinical, and outcome data. All specimens are evaluated for pathologic diagnosis by CBCTR pathologists using standard diagnostic criteria. The collection is particularly well suited for validation studies of diagnostic and prognostic markers. The CBCTR also makes available breast cancer tissue microarrays designed by NCI statisticians to provide high statistical power for studies of stage-specific markers of breast cancer. Contact CBCTR’s Web site at http://cbctr.nci.nih.gov, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com. NCI - Cooperative Prostate Cancer Tissue Resource (CPCTR) The NCI Cooperative Prostate Cancer Tissue Resource (CPCTR) can provide access to over 4,000 cases of formalin-fixed, paraffin-embedded primary prostate cancer specimens, with associated pathology and clinical data. Fresh-frozen tissue is also available with limited clinical follow-up information. In addition, slides from prostate cancer tissue microarrays with associated pathology and clinical data are now available. Contact the CPCTR Web site at http://www.prostatetissues.org, or contact Steve Marroulis at Information Management Services, Inc.: telephone: (301) 680-9770; e-mail: marrouliss@imsweb.com. NCI - AIDS and Cancer Specimen Resource (ACSR) The AIDS and Cancer Specimen Resource (ACSR) provides qualified researchers with tissue, cell, blood, and fluid specimens, as well as clinical data from patients with AIDS and cancer. The specimens and clinical data are available for research studies, particularly those that translate basic research findings to clinical application. Contact the ACSR Web site (http://acsr.ucsf.edu/) or Dr. Kishor Bhatia, (301) 496-7147; e-mail: bhatiak@mail.nih.gov. NCI - Breast and Ovarian Cancer Family Registries (CFRs) The Breast and Ovarian CFRs facilitate and support interdisciplinary and population-based research on the identification and characterization of breast and ovarian cancer susceptibility genes, with particular emphasis on gene-gene and gene-environment interaction research. Available from the registries are: a) family history, epidemiologic and clinical data, b) updates on cancer recurrence, morbidity and mortality in participating families, and c) biospecimens, including plasma, lymphocytes, serum, DNA, Guthrie cards or buccal smears, and paraffin blocks of tumor tissue. For further information on these registries, contact the CFR Web site (http://epi.grants.cancer.gov/BCFR) or (301) 496-9600. NCI - Specimen Resource Locator The NCI Specimen Resource Locator (http://cancer.gov/specimens) is a database that helps researchers locate specimens for research. The database includes resources such as tissue banks and tissue procurement systems with access to normal, benign, precancerous, and/or cancerous human tissue covering a wide variety of organ sites. Researchers specify the types of specimens, number of cases, preservation methods, and associated data they require. The Locator will search the database and return a list of tissue resources most likely to meet their requirements. When no match is obtained, the researcher is referred to the NCI Tissue Expediter [(301) 496-7147; e-mail: tissexp@mail.nih.gov]. The Tissue Expediter is a scientist who can help match researchers with appropriate resources or identify appropriate collaborators when those are necessary. NIDDK - Biologic Samples from Diabetic Study Foundation A portion (1/3) of all stored nonrenewable samples (plasma, serum, urine) from subjects enrolled in the Diabetes Control and Complications Trial (DCCT) is available for use by the scientific community to address questions for which these samples may be invaluable. Announcements for using this resource appear in the NIH Guide for Grants and Contracts periodically. Inquiries may be addressed to: Catherine C. Cowie, Ph.D., Director, Diabetes Epidemiology Program, NIDDK, 6707 Democracy Blvd., Room 691, MSC 5460, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892-5460. Phone: (301) 594-8804; fax: (301) 480-3503; e-mail: cowiec@extra.niddk.nih.gov. NIDDK - NIDDK Central Repositories (Diabetes Prevention Study) The NIDDK Central Repositories have selected biosamples from the DPT-1 (The Diabetes Prevention Type 1) study that are available to qualified investigators through an application process. These samples are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. Information about how to apply for these materials can be obtained from the NIDDK Central Repositories by contacting Ms. Helen Ray of RTI, 1-919-316-3418, or hmp@rti.org. Direct scientific-technical inquiry to the Project Officer of the NIDDK Central Repositories, Dr. Rebekah Rasooly, at phone: (301) 594-6007; e-mail: rr185i@nih.gov. Visit the Repositories Web site at http://www.niddkrepository.org. NICHD - Brain and Tissue Bank for Developmental Disorders The purpose of the Bank is to collect, preserve, and distribute human tissues to investigators interested in autism and developmental disorders; normal tissues may be available for other research purposes. Further information can be obtained at www.btbank.org. The contact persons are H. Ron Zielke or Sally Wisniewsky, University of Maryland (1-800-847-1539), and Carol Petito or Stephanie Lojko, University of Miami (1-800-592-7246). NICHD - Reproductive Tissue Sample Repository (RTSaR) The Reproductive Tissue Sample Repository (RTSaR) is a virtual repository with online tissue sample acquisition capabilities. The RTSaR provides investigators with real-time access to human and nonhuman primate tissue and fluid inventories from four tissue bank facilities that are supported through the Specialized Cooperative Centers Program in Reproduction Research. The tissue banks are located at the University of California, San Diego (human ovary bank), Stanford University (human endometrium and DNA bank), Johns Hopkins University (male reproductive tissues and fluids), and the Oregon National Primate Research Center (nonhuman primate tissues). The web site for the RTSaR is https://rtsar.nichd.nih.gov/rtsar/login. If you wish to access the RTSaR, you can request an id and password to access the system by contacting the network administrator at RTSaR@mail.nih.gov. Once you access the system, contact information for each bank is provided. Access is open to all investigators living in North America who are supported by research and research training grants from the NIH. One id and password will be provided to each principal investigator that can be utilized by any person working in the P.I.’s laboratory, or, in the case of institutional training grants (T32) and institutional career development award programs (K12), any person supported by the aforementioned awards. NCRR - Human Tissues and Organs Resource (HTOR) The Human Tissues and Organs Resource (HTOR) cooperative agreement supports a procurement network developed by the National Disease Research Interchange (NDRI), a not-for-profit organization. By collaborating with various medical centers, hospitals, pathology services, eye banks, tissue banks, and organ procurement organizations, HTOR provides a wide variety of human tissues and organs—both diseased and normal—to researchers for laboratory studies. Such samples include tissues from the central nervous system and brain, cardiovascular system, endocrine system, eyes, bone, and cartilage. For further information, consult the NDRI Web site (www.ndri.com) or contact Dr. John T. Lonsdale at NDRI, 8 Penn Center, 8th Floor, 1628 JFK Boulevard, Philadelphia, PA 19103. Phone: (800) 222-6374, ext. 271; fax: (215) 557-7154; e-mail: jlonsdale@ndriresource.org. The NDRI Web site is http://www.ndri.com. NCRR - Islet Cell Resource (ICR) With support from NCRR, 10 Islet Cell Resource (ICR) centers isolate, purify, and characterize human pancreatic islets for subsequent transplantation into patients with type I diabetes. The ICR centers procure whole pancreata and acquire relevant data about donors; improve islet isolation and purification techniques; distribute islets for use in approved clinical protocols; and perfect the methods of storage and shipping. In this way, the centers optimize the viability, function, and availability of islets and help clinical researchers capitalize on the recently reported successes in islet transplantation. Information on submitting requests for islet cells can be obtained from Mr. John Kaddis, ICR Coordinating Center Project Manager, City of Hope National Medical Center, 1500 E. Duarte Road, Duarte, California 91010. Phone (626) 359-8111, ext. 63377; fax: (626) 471-7106; e-mail: jkaddis@coh.org. The Coordinating Center hosts a Web site at http://icr.coh.org. NIA - SWAN Repository (longitudinal, multiethnic study of women at midlife including the menopausal transition) The SWAN Repository is a biologic specimen bank of the Study of Women’s Health Across the Nation (SWAN). The SWAN cohort was recruited in 1996/1997 and consists of 3302 African-American, Caucasian, Chinese, Hispanic, and Japanese women. The SWAN Repository contains more than 350,000 blood and urine specimens generated from the study participants’ annual visits (8 visits to date), at which time medical and health history, psychosocial measures, biological measures, and anthropometric data were and are being collected. In addition, a subset of the participants are providing urine samples, collected daily over the length of one menstrual cycle, each year. More than 900,000 of these samples are in the SWAN Repository and are available to researchers who wish to study the midlife and menopausal transition. Additionally, a DNA sample repository is also available and includes DNA as well as transformed B-lymphoblastoid cell lines from more than 1800 of the participants. To learn more about the SWAN Repository and how to apply to use SWAN Repository specimens, contact the Web site at http://www.swanrepository.com or Dr. MaryFran Sowers, University of Michigan, School of Public Health, Epidemiology Dept., (734) 936-3892; e-mail: mfsowers@umich.edu. HUMAN AND ANIMAL CELL AND BIOLOGIC REAGENT RESOURCES NIDDK - National Hormone and Peptide Program The National Hormone and Peptide Program (NHPP) offers peptide hormones and their antisera, tissues (rat hypothalami), and miscellaneous reagents to qualified investigators. These reagents are supplied for research purposes only, not for therapeutic, diagnostic, or commercial uses. These materials can be obtained from Dr. A. F. Parlow of the Harbor-UCLA Medical Center, Research and Education Institute, Torrance, CA. A more complete description of resources within this program is provided in The Endocrine Society journals. Direct scientific-technical inquiry to NHPP Scientific Director, Dr. Al Parlow, at phone: (310) 222-3537; fax: (310) 222-3432; e-mail: parlow@humc.edu. Visit the NHPP Web site at http://www.humc.edu/hormones. NICHD - National Hormone and Pituitary Program (see NIDDK listing) Following is a list of reagents currently available through the resources of NICHD: Androgen receptor and peptide antigen Recombinant monkey (cynomolgus) and baboon luteinizing hormone and follicle-stimulating hormone and antisera. NIA - Aging Cell Bank To facilitate aging research on cells in culture, the NIA provides support for the Aging Cell Bank located at the Coriell Institute for Medical Research in Camden, NJ. The Aged Cell Bank provides fibroblast, lymphoblastoid, and differentiated cell lines from a wide range of human age-related conditions and other mammalian species, as well as DNA from a limited subset of cell lines. For further information, the Aged Cell Bank catalog can be accessed at http://locus.umdnj.edu/nia or contact Dr. Donald Coppock at 1-800-752-3805. NCRR - Various Cell Repositories NCRR maintains the following cell repository resources: National Cell Culture Center, National Stem Cell Resource, and the Yeast Genetic Stock Center. Further information regarding these resources may be obtained through the NCRR Web site at: www.ncrr.nih.gov/ncrrprog/cmpdir/BIOLOG.asp. ANIMAL RESOURCES NIA - Aging Rodent Resources NIA maintains both rat and mouse colonies for use by the scientific community. The animals available range in age from 1 to 36 months. A repository of fresh-frozen tissue from the NIA aged rodent colonies is stocked with tissue from mouse and rat strains, including caloric-restricted BALB/c mice. The NIA also maintains a colony of calorically restricted rodents of selected genotypes, which are available to the scientific community. For further information, please refer to the Aged Rodent information handbook at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentColoniesHandbook/ or contact the Office of Biological Resources and Resource Development order desk. Phone: (301) 496-0181; fax: (301) 402-5597; e-mail: rodents@nia.nih.gov. NIA - Aged Rodent Tissue Bank The rodent tissue bank contains flash-frozen tissues from rodents in the NIA aged rodent colonies. Tissue is collected from rodents at 4 or 5 age points throughout the lifespan. Tissue arrays are also available. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentTissueBankHandbook/. NCRR - Mutant Mouse Regional Resource Centers (MMRRC) The Mutant Mouse Regional Resource Center (MMRRC) Program consists of centers that collectively operate as a one-stop shop to serve the biomedical research community. Investigators who have created select mutant mouse models may donate their models to an MMRRC for broad dissemination to other investigators who request them for noncommercial research investigations related to human health, disease, and treatments. The NCRR Division of Comparative Medicine (DCM) supports the MMRRCs, which are electronically linked through the MMRRC Informatics Coordinating Center (ICC) to function as one facility. The ICC, located at The Jackson Laboratory in Bar Harbor, ME, provides database and other informatics support to the MMRRC to give the research community a single entry point to the program. Further information can be obtained from the Web site at http://www.mmrrc.org, or from Franziska Grieder, D.V.M., Ph.D., Division of Comparative Medicine, NCRR. Phone (301) 435-0744; fax: (301) 480-3819; e-mail: griederf@ncrr.nih.gov. NCRR - Induced Mutant Mouse Resource (IMR) The Induced Mutant Mouse Resource (IMR) at The Jackson Laboratory provides researchers with genetically engineered mice (transgenic, targeted mutant, retroviral insertional mutant, and chemically induced mutant mice). The function of the IMR is to select, import, cryopreserve, maintain, and distribute these important strains of mice to the research community. To improve their value for research, the IMR also undertakes genetic development of stocks, such as transferring mutant genes or transgenes to defined genetic backgrounds and combining transgenes and/or targeted mutations to create new mouse models for research. Over 800 mutant stocks have been accepted by the IMR. Current holdings include models for research on cancer, immunological and inflammatory diseases, neurological diseases and behavioral disorders, cardiovascular diseases, developmental disorders, metabolic and other diseases, reporter (e.g. GFP) and recombinase (e.g. cre/loxP) strains. About 8 strains a month are being added to the IMR holdings. A list of all strains may be obtained from the IMR Web site: www.jax.org/resources/documents/imr/. Online submission forms are also available on that site. All mice can be ordered by calling The Jackson Laboratory’s Customer Service Department at 1-800-422-MICE or (207) 288-5845 or by faxing (207) 288-6150. NIDDK - Mouse Metabolic Phenotyping Centers The mission of the Mouse Metabolic Phenotyping Centers is to provide the scientific community with standardized, high-quality metabolic and physiologic phenotyping services for mouse models of diabetes, diabetic complications, obesity, and related disorders. Researchers can ship mice to one of the four Centers (University of Cincinnati, University of Texas Southwestern Medical Center, Vanderbilt University, and Yale University) and obtain on a fee-for-service basis a range of complex exams used to characterize mouse metabolism, blood composition, energy balance, eating and exercise, organ function and morphology, physiology, and histology. Many tests are done in living animals and are designed to elucidate the subtle hallmarks of metabolic disease. Information, including a complete list of available tests, can be found at www.mmpc.org, or contact Dr. Maren R. Laughlin, NIDDK, at (301) 594-8802; e-mail: Maren.Laughlin@nih.gov; or Dr. Kristin Abraham, NIDDK, at (301) 451-8048; e-mail: abrahamk@extra.niddk.nih.gov. NCRR - National Primate Research Centers (NPRCs) National Primate Research Centers (NPRCs) are a network of eight highly specialized facilities for nonhuman primates (NHP) research. Funded by grants through NCRR’s Division of Comparative Medicine (DCM), each center, staffed with experienced research and support staff, provides the appropriate research environment to foster the development of NHP models of human health and disease for biomedical investigations. The NPRCs are affiliated with academic institutions and are accessible to eligible biomedical and behavioral investigators supported by research project grants from the National Institutes of Health and other sources. Further information may be obtained from the notice, Procedures for Accessing Regional Primate Research Centers, published in the NIH Guide for Grants and Contracts at http://grants2.nih.gov/grants/guide/notice-files/not97-014.html, or from John Harding, Ph.D., National Primate Research Centers and AIDS Animal Models Program, Division of Comparative Medicine, NCRR. Phone: (301) 435-0744; fax: (301) 480-3819; e-mail: hardingj@mail.nih.gov. NIA - Nonhuman Primates, Aging Set-Aside Colony NIA maintains approximately 200 nonhuman primates (M. mulatta) at four National Primate Research Centers (see above) for conducting research on aging. These animals range in age from 18 to 35 years. While these animals are predominantly reserved for non-invasive research, exceptions can be made to this policy. For further information, please contact Dr. Nancy Nadon, Office of Biological Resources and Resource Development, NIA. Phone: (301) 402-7744; fax: (301) 402-0010; e-mail: nadonn@nia.nih.gov. NIA - Nonhuman Primate (NHP) Tissue Bank and Aging Database The NIA developed two new resources to facilitate research in the NHP model. The NHP tissue bank contains fresh-frozen and fixed tissue donated by primate centers around the country. Information is available at http://www.nia.nih.gov/ResearchInformation/ScientificResources/NHPTissueBankHandbook.htm. The Primate Aging Database provides an internet accessible database with data from thousands of primates around the country. It can be used to investigate the effect of age on a variety of parameters, predominantly blood chemistry and husbandry measurements. The site is password protected. The URL is http://ipad.primate.wisc.edu. NIA - Obesity, Diabetes and Aging Animal Resource (USF-ODARC) The NIA supports a colony of aged rhesus macaques, many of which are obese and/or diabetic. This is a long-term colony of monkeys housed at the University of South Florida’s Obesity, Diabetes and Aging Research Center. They have been extensively and longitudinally characterized for general health variables, blood chemistry, food intake, and body weight. Diabetic monkeys are tested daily for urine glucose and ketone levels, and prediabetic monkeys are tested weekly. Data for some of the monkeys extend as far back as 15 years. This unique resource is available for collaborative studies. ODARC has a significant amount of stored tissue collected at necropsy and stored blood/plasma collected longitudinally. Serial blood collection or tissue collection at necropsy can also be performed prospectively. Testing and imaging can also be performed on the monkeys. Inquiries regarding collaborative studies using the ODARC colony should be directed to: Barbara C. Hansen, Ph.D., Director, Obesity, Diabetes and Aging Research Center, University of South Florida, All Children’s Hospital, 801 6th Street South #9340, St. Petersburg, FL 33701. Phone: (727) 767-6993; fax: (727) 767-7443; e-mail: bchansen@aol.com. NCRR - Various Animal Resources NCRR maintains the following animal resources: Animal Models and Genetic Stocks, Chimpanzee Biomedical Research Program, NIH Animal Genetic Resource, and the Specific Pathogen Free Macaque Breeding and Research Program. Further information regarding these and other resources may be obtained through the NCRR Web site at www.ncrr.nih.gov/comparative_med.asp. MISCELLANEOUS RESOURCES NCRR - National Gene Vector Laboratories (NGVLs) The National Gene Vector Laboratories (NGVLs), with core funding from NCRR, serve as a resource for researchers to obtain adequate quantities of clinical-grade vectors for human gene transfer protocols. The vector types include retrovirus, lentivirus, adenovirus, adeno-associated virus, herpes-virus, and DNA plasmids. The NGVLs consist of three vector production centers at: Baylor College of Medicine; City of Hope National Medical Center and Beckman Research Institute; and Indiana University, which also serves as the Coordinating Center for all the laboratories. Two additional laboratories conduct toxicology studies for NGVL-approved investigators. These laboratories are located at the Southern Research Institute and the University of Florida. Additional information about the process for requesting vector production and/or pharmacology/toxicology support should be directed to Ms. Lorraine Matheson, NGVL Project Coordinator, Indiana University School of Medicine. Phone: (317) 274-4519; fax: (317) 278-4518; e-mail: lrubin@iupui.edu. The NGVL Coordinating Center at Indiana University also hosts a Web site at http://www.ngvl.org. NCRR - General Clinical Research Centers (GCRCs) The General Clinical Research Centers (GCRCs) are a national network of 82 centers that provide optimal settings for medical investigators to conduct safe, controlled, state-of-the-art in-patient and out-patient studies of both children and adults. GCRCs also provide infrastructure and resources that support several career development opportunities. Investigators who have research project funding from the National Institutes of Health (NIH) and other peer-reviewed sources may apply to use GCRCs. Because the GCRCs support a full spectrum of patient-oriented scientific inquiry, researchers who use these centers can benefit from collaborative, multidisciplinary research opportunities. To request access to a GCRC facility, eligible investigators should initially contact a GCRC program director, listed in the National Center for Research Resources (NCRR) Clinical Research Resources Directory (www.ncrr.nih.gov/ncrrprog/clindir/crdirectory.asp). Further information can be obtained from Anthony R. Hayward, M.D., Director, Division for Clinical Research Resources, National Center for Research Resources at NIH. Phone: (301) 435-0790; e-mail: haywarda@ncrr.nih.gov.

In Australian universities, many courses provide lecture notes as a standard learning resource; however, captions and transcripts of these lectures are not usually provided unless requested by a student through dedicated disability support officers (Worthington). As a result, to date their use has been limited. However, while the requirement for—and benefits of—captioned online lectures for students with disabilities is widely recognised, these captions or transcripts might also represent further opportunity for a personalised approach to learning for the mainstream student population (Podszebka et al.; Griffin). This article reports findings of research assessing the usefulness of captioned recorded lectures as a mainstream learning tool to determine their usefulness in enhancing inclusivity and learning outcomes for the disabled, international, and broader student population.Literature ReviewCaptions have been found to be of benefit for a number of different groups considered at-risk. These include people who are D/deaf or hard of hearing, those with other learning difficulties, and those from a non-English speaking background (NESB).For students who are D/deaf or hard of hearing, captions play a vital role in providing access to otherwise inaccessible audio content. Captions have been found to be superior to sign language interpreters, note takers, and lip reading (Stinson et al.; Maiorana-Basas and Pagliaro; Marschark et al.).The use of captions for students with a range of cognitive disabilities has also been shown to help with student comprehension of video-based instruction in a higher education context (Evmenova; Evmenova and Behrmann). This includes students with autism spectrum disorder (ASD) (Knight et al.; Reagon et al.) and students with dyslexia (Alty et al.; Beacham and Alty). While, anecdotally, captions are also seen as of benefit for students with attention deficit hyperactivity disorder (ADHD) (Kent et al.), studies have proved inconclusive (Lewis and Brown).The third group of at-risk students identified as benefiting from captioning recorded lecture content are those from a NESB. The use of captions has been shown to increase vocabulary learning (Montero Perez, Peters, Clarebout, and Desmet; Montero Perez, Van Den Noortgate, and Desmet) and to assist with comprehension of presenters with accents or rapid speech (Borgaonkar, 2013).In addition to these three main groups of at-risk students, captions have also been demonstrated to increase the learning outcomes for older students (Pachman and Ke, 2012; Schmidt and Haydu, 1992). Captions also have demonstrable benefits for the broader student cohort beyond these at-risk groups (Podszebka et al.; Griffin). For example, a recent study found that the broader student population utilised lecture captions and transcripts in order to focus, retain information, and overcome poor audio quality (Linder). However, the same study revealed that students were largely unaware about the availability of captions and transcripts, nor how to access them.MethodologyIn 2016 students in the Curtin University unit Web Communications (an introductory unit for the Internet Communications major) and its complementary first year unit, Internet and Everyday Life, along with a second year unit, Web Media, were provided with access to closed captions for their online recorded lectures. The latter unit was added to the study serendipitously when its lectures were required to be captioned through a request from the Curtin Disability Office during the study period. Recordings and captions were created using the existing captioning system available through Curtin’s lecture recording platform—Echo360. As well as providing a written caption of what is being said during the lectures, this system also offers a sophisticated search functionality, as well as access to a total transcript of the lecture. The students were provided access to an online training module, developed specifically for this study, to explain the use of this system.Enrolled Curtin students, both on-campus and online, Open Universities Australia (OUA) students studying through Curtin online, teaching staff, and disability officers were then invited to participate in a survey and interviews. The study sought to gain insights into students’ use of both recorded lectures and captioned video at the time of the survey, and their anticipated future usage of these services (see Kent et al.).A total of 50 students—of 539 enrolled across the different instances of the three units—completed the survey. In addition, five follow-up interviews with students, teaching staff, and disability support staff were conducted once the surveys had been completed. Staff interviewed included tutors and unit coordinators who taught and supervised units in which the lecture captions were provided. The interviews assessed the awareness, use, and perceived validity of the captions system in the context of both learning and teaching.ResultsA number of different questions were asked regarding students’ demographics, their engagement with online unit materials, including recorded lectures, their awareness of Echo360’s lecture captions, as well as its additional features, their perceived value of online captions for their studies, and the future significance of captions in a university context.Of the 50 participants in the survey, only six identified themselves as a person with a disability—almost 90 per cent did not identify as disabled. Additionally, 45 of the 50 participants identified English as their primary language. Only one student identified as a person with both a disability and coming from a NESB.Engagement with Online Unit Materials and Recorded LecturesThe survey results provide insight into the ways in which participants interact with the Echo360 lecture system. Over 90 per cent of students had accessed the recorded lectures via the Echo360 system. While this might not seem notable at first, given such materials are essential elements of the units surveyed, the level of repeated engagement seen in these results is important because it indicates the extent to which students are revising the same material multiple times—a practice that captions are designed to facilitate and assist. For instance, one lecture was recorded per week for each unit surveyed, and most respondents (70 per cent) were viewing these lectures at least once or twice a week, while 10 per cent were viewing the lectures multiple times a week. Over half of the students surveyed reported viewing the same lecture more than once. Out these participants, 19 (or 73 per cent) had viewed a lecture twice and 23 per cent had viewed it three times or more. This illustrates that frequent revision is taking place, as students watch the same lecture repeatedly to absorb and clarify its contents. This frequency of repeated engagement with recorded unit materials—lectures in particular—indicates that students were making online engagement and revision a key element of their learning process.Awareness of the Echo360 Lecture Captions and Additional FeaturesHowever, while students were highly engaged with both the online learning material and the recorded lectures, there was less awareness of the availability of the captioning system—only 34 per cent of students indicated they were aware of having access to captions. The survey also asked students whether or not they had used additional features of the Echo360 captioning system such as the search function and downloadable lecture transcripts. Survey results confirm that these features were being used; however, responses indicated that only a minority of students using the captions system used these features, with 28 per cent using the search function and 33 per cent making use of the transcripts. These results can be seen as an indication that additional features were useful for revision, albeit for the minority of students who used them. A Curtin disability advisor noted in their interview that:transcripts are particularly useful in addition to captions as they allow the user to quickly skim the material rather than sit through a whole lecture. Transcripts also allow translation into other languages, highlighting text and other features that make the content more accessible.Teaching staff were positive about these features and suggested that providing transcripts saved time for tutors who are often approached to provide these to individual students:I typically receive requests for lecture transcripts at the commencement of each study period. In SP3 [during this study] I did not receive any requests.I feel that lecture transcripts would be particularly useful as this is the most common request I receive from students, especially those with disabilities.I think transcripts and keyword searching would likely be useful to many students who access lectures through recordings (or who access recordings even after attending the lecture in person).However, the one student who was interviewed preferred the keyword search feature, although they expressed interest in transcripts as well:I used the captions keyword search. I think I would like to use the lecture transcript as well but I did not use that in this unit.In summary, while not all students made use of Echo360’s additional features for captions, those who did access them did so frequently, indicating that these are potentially useful learning tools.Value of CaptionsOf the students who were aware of the captions, 63 per cent found them useful for engaging with the lecture material. According to one of the students:[captions] made a big difference to me in terms on understanding and retaining what was said in the lectures. I am not sure that many students would realise this unless they actually used the captions…I found it much easier to follow what was being said in the recorded lectures and I also found that they helped stay focussed and not become distracted from the lecture.It is notable that the improvements described above do not involve assistance with hearing or language issues, but the extent to which captions improve a more general learning experience. This participant identified themselves as a native English speaker with no disabilities, yet the captions still made a “big difference” in their ability to follow, understand, focus on, and retain information drawn from the lectures.However, while over 60 per cent of students who used the captions reported they found them useful, it was difficult to get more detailed feedback on precisely how and why. Only 52.6 per cent reported actually using them when accessing the lectures, and a relatively small number reported taking advantage of the search and transcripts features available through the Echo360 system. Exactly how they were being used and what role they play in student learning is therefore an area to pursue in future research, as it will assist in breaking down the benefits of captions for all learners.Teaching staff also reported the difficulty in assessing the full value of captions—one teacher interviewed explained that the impact of captions was hard to monitor quantitatively during regular teaching:it is difficult enough to track who listens to lectures at all, let alone who might be using the captions, or have found these helpful. I would like to think that not only those with hearing impairments, but also ESL students and even people who find listening to and taking in the recording difficult for other reasons, might have benefitted.Some teaching staff, however, did note positive feedback from students:one student has given me positive feedback via comments on the [discussion board].one has reported that it helps with retention and with times when speech is soft or garbled. I suspect it helps mediate my accent and pitch!While 60 per cent claiming captions were useful is a solid majority, it is notable that some participants skipped this question. As discussed above, survey answers indicate that this was because these 37 students did not think they had access to captions in their units.Future SignificanceOverall, these results indicate that while captions can provide a benefit to students’ engagement with online lecture learning material, there is a need for more direct and ongoing information sharing to ensure both students and teaching staff are fully aware of captions and how to use them. Technical issues—such as the time delay in captions being uploaded—potentially dissuade students from using this facility, so improving the speed and reliability of this tool could increase the number of learners keen to use it. All staff interviewed agreed that implementing captions for all lectures would be beneficial for everyone:any technology that can assist in making lectures more accessible is useful, particularly in OUA [online] courses.it would be a good example of Universal Design as it would make the lecture content more accessible for students with disabilities as well as students with other equity needs.YES—it benefits all students. I personally find that I understand and my attention is held more by captioned content.it certainly makes my role easier as it allows effective access to recorded lectures. Captioning allows full access as every word is accessible as opposed to note taking which is not verbatim.DiscussionThe results of this research indicate that captions—and their additional features—available through the Echo360 captions system are an aid to student learning. However, there are significant challenges to be addressed to make students aware of these features and their potential benefits.This study has shown that in a cohort of primarily English speaking students without disabilities, over 60 per cent found captions a useful addition to recorded lectures. This suggests that the implementation of captions for all recorded lectures would have widespread benefits for all learners, not only those with hearing or language difficulties. However, at present, only “eligible” students who approach the disability office would be considered for this service, usually students who are D/deaf or hard of hearing. Yet it can be argued that these benefits—and challenges—could also extend to other groups that are might traditionally have been seen to benefit from the use of captions such as students with other disabilities or those from a NESB.However, again, a lack of awareness of the training module meant that this potential cohort did not benefit from this trial. In this study, none of the students who identified as having a disability or coming from a NESB indicated that they had access to the training module. Further, five of the six students with disabilities reported that they did not have access to the captions system and, similarly, only two of the five NESB students. Despite these low numbers, all the students who were part of these two groups and who did access the captions system did find it useful.It can therefore be seen that the main challenge for teaching staff is to ensure all students are aware of captions and can access them easily. One option for reducing the need for training or further instructions might be having captions always ON by default. This means students could incorporate them into their study experience without having to take direct action or, equally, could simply choose to switch them off.There are also a few potential teething issues with implementing captions universally that need to be noted, as staff expressed some concerns regarding how this might alter the teaching and learning experience. For example:because the captioning is once-off, it means I can’t re-record the lectures where there was a failure in technology as the new versions would not be captioned.a bit cautious about the transcript as there may be problems with students copying that content and also with not viewing the lectures thinking the transcripts are sufficient.Despite these concerns, the survey results and interviews support the previous findings showing that lecture captions have the potential to benefit all learners, enhancing each student’s existing capabilities. As one staff member put it:in the main I just feel [captions are] important for accessibility and equity in general. Why should people have to request captions? Recorded lecture content should be available to all students, in whatever way they find it most easy (or possible) to engage.Follow-up from students at the end of the study further supported this. As one student noted in an email at the start of 2017:hi all, in one of my units last semester we were lucky enough to have captions on the recorded lectures. They were immensely helpful for a number of reasons. I really hope they might become available to us in this unit.ConclusionsWhen this project set out to investigate the ways diverse groups of students could utilise captioned lectures if they were offered it as a mainstream learning tool rather than a feature only disabled students could request, existing research suggested that many accommodations designed to assist students with disabilities actually benefit the entire cohort. The results of the survey confirmed this was also the case for captioning.However, currently, lecture captions are typically utilised in Australian higher education settings—including Curtin—only as an assistive technology for students with disabilities, particularly students who are D/deaf or hard of hearing. In these circumstances, the student must undertake a lengthy process months in advance to ensure timely access to essential captioned material. Mainstreaming the provision of captions and transcripts for online lectures would greatly increase the accessibility of online learning—removing these barriers allows education providers to harness the broad potential of captioning technology. Indeed, ensuring that captions were available “by default” would benefit the educational outcomes and self-determination of the wide range of students who could benefit from this technology.Lecture captioning and transcription is increasingly cost-effective, given technological developments in speech-to-text or automatic speech recognition software, and the increasing re-use of content across different iterations of a unit in online higher education courses. At the same time, international trends in online education—not least the rapidly evolving interpretations of international legislation—provide new incentives for educational providers to begin addressing accessibility shortcomings by incorporating captions and transcripts into the basic materials of a course.Finally, an understanding of the diverse benefits of lecture captions and transcripts needs to be shared widely amongst higher education providers, researchers, teaching staff, and students to ensure the potential of this technology is accessed and used effectively. Understanding who can benefit from captions, and how they benefit, is a necessary step in encouraging greater use of such technology, and thereby enhancing students’ learning opportunities.AcknowledgementsThis research was funded by the Curtin University Teaching Excellence Development Fund. Natalie Latter and Kai-ti Kao provided vital research assistance. 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IntroductionIn 2016, the online cause #Mission22 went viral on social media. Established to raise awareness about high suicide rates among US military veterans, the campaign involves users posting a video of themselves doing 22 push-ups for 22 days, and on some platforms, to donate and recruit others to do the same. Based on a ‘big data’ analysis of Twitter data (over 225,883 unique tweets) during the height of the campaign, this article uses #Mission22 as a site in which to analyse how people depict, self-represent and self-tell as moral subjects using social media campaigns. In addition to spotlighting how such movements are mobilised to portray moral selves in particular ways, the analysis focuses on how a specific online cause like #Mission22 becomes popularly supported from a plethora of possible causes and how this selection and support is shaped by online networks. We speculate that part of the reason why Mission22 went ‘viral’ in the highly competitive attention economies of social media environments was related to visual depictions of affective bodily, fitness and moral practices.Web 2.0 Culture: Self and Mass DepictionWeb 2.0 culture such as social networking sites (eg., Facebook; Instagram), the advent of video sharing technologies (eg., YouTube) and more recently, micro-blogging services like Twitter have created new and transformative spaces to create, depict and display identity. Web 2.0 is primarily defined by user-generated content and interaction, whereby users are positioned as both consumer and producers, or ‘produsers’ of Web content (Bruns and Schmidt). Challenging traditional “broadcast” media models, Web 2.0 gives users a platform to produce their own content and for “the many” to communicate “with the many” (Castells). The growth of mass self communication, supported by broadband and wireless technologies, gives unprecedented power to individuals and groups to depict and represent their identities and relationships to a potential global audience.The rise of user-generated communication technologies dovetails with broader analyses of the changing contours of self and identity in late-modern times. Individuals in the early decades of the 21st century must take charge for how they depict, portray and self-tell as distinctive, unique and individual subjects (Beck and Beck-Gernsheim; Giddens; Bauman). As contemporary lives become less bound to the strictures of tradition, community and religion, the self becomes a project to be worked out and developed. These theorists suggest that via processes of individualisation, detraditionalisation and globalisation, contemporary subjects have become disconnected from the traditional coordinates of community and are thus faced with the imperative of self-construction and reinvention (Elliott and Lemert).More recently, theoretical and empirical work has attempted to interpret and evaluate how networks of mass self-depiction powered by new digital and wireless technologies are reshaping identity practices. For some theorists, like Bauman (Consuming 2) and Turkle, Web 2.0 is a worrying trend. Bauman suggests in the “confessional society” – think reality TV, talk shows, social media – people are compelled to curate and reflect upon their lives in the public realm. These public acts of self-depiction are part of a move to treating the self as a brand to be consumed, “as products capable of drawing attention, and attracting demands and customers” (Bauman and Lyon 33). The consumer quality of new communications sees connections replace relationships as social bonds become short-term and brittle. Turkle makes a similar argument, suggesting that our preoccupation with online curation centres on controlling our identities and depicting “perfect” versions of ourselves. The result is diminished forms of intimacy and connection; we preach authenticity and realness but practice self-curation and self-stylisation.A more positive body of literature has examined how Web technologies work as tools for the formation of self. This literature is based on more close-up and detailed readings of particular platforms and practices rather than relying on sweeping claims about technology and social change. Following Foucault, Bakardjieva & Gaden argue that personal blogs and social networking site (SNS) profiles constitute a contemporary technology of the self, whereby users employ Web 2.0 technologies in everyday life as practices of self care and self-formation. In a similar way, Sauter argues that SNSs, and in particular Facebook, are tools for self-formation through the way in which status updates provide a contemporary form of self-writing. Eschewing the notion of social media activity as narcissistic or self-obsessive, Sauter argues that SNSs are a techno-social practice of self-writing that facilitate individuals to “form relations to self and others by exposing themselves to others and obtaining their feedback” (Sauter 836). Other research has explored young people’s sustained use of social media, particularly Facebook, and how these sites are used to tell and archive “growing up” narratives and key rites of passage (Robards and Lincoln).One area of research that has been overlooked is how people use social media to construct and depict moral identity. Following Sauter’s arguments about the self work that occurs through networked self-writing, we can extend this to include the ethical self work performed and produced through online depictions. One exception is work by Hookway which analyses how people use blogs – an earlier Web 2.0 form – to write and self-examine their moral experiences. This research shows how bloggers use blogging as a form of online self-writing to construct a do-it-yourself form of morality that emphasises the self, emotions, body and ideals of authenticity. Hookway highlights the idea that morality is less about obedience to a code of rules or following external laws to becoming a particular moral person through a set of self-practices. Paralleling broader shifts in identity construction, people are no longer bound to the inherited guidelines of the past, morality becomes a project to be worked out, designed and depicted in relation to Others (Hookway).In Foucault’s terms, morality involves a process of ethical self-stylisation – an “aesthetics of existence” – based on “the ethical work of the self on the self” (Foucault 91). “Care of the self” involves a “set of occupations” or “labours” that connect and link the self to the Other through guidance, counselling and communication (Foucault 50). For Foucault, self-creation and self-care imply “care for others” as individuals perform a mutual concern with achieving an “art of existence”. This is a reciprocated ethics that obligates the individual to care for others in order to help them care for themselves.This stylisation of the ethical self has been drastically reshaped by the new opportunities for self-expression, belonging and communication offered in our digitally networked society. Digital worlds and spaces create new multi-media modes for individuals and groups to depict, perform and communicate particular moral identities and positions. Web 2.0 technologies are seeing the boundaries between the private and public sphere collapse as more people are willing to share the most intimate part of their moral lives with a diverse mix of strangers, friends, family and associates.The confessional quality of online spaces provide a unique opportunity to analyse “lay morality” or everyday moral understandings, constructions and depictions and how this is co-produced in relation to new technological affordances. Following Sayer (951), morality is defined as “how people should treat others and be treated by them, which of course is crucial for their subjective and objective well-being”. Morality is understood as a relational and evaluative practice that involves being responsive to how people are faring and whether they are suffering or flourishing.In this article, we use the #Mission22 campaign – a campaign that went “viral” across multiple social media platforms – as a unique site to analyse and visualise lay moral depictions and constructions. Specifically, we analyse the #Mission22 campaign on Twitter using a big data analysis. Much of the empirical work on online self construction and depiction is either purely theoretical in the vein of Bauman, Turkle and Sauter or based on small qualitative samples such as the work by Lincoln and Robards and Author A. This article is unique not only in investigating the crafting of moral depictions in Web 2.0 forums but also in the scale of the textual and visual representation of mass moral self-depictions it captures and analyses. Big Data Analysis of #Mission22 on TwitterIn order to empirically examine the #Mission22 campaign on Twitter, we used the Twitter API to collect over three months of tweets that contained the campaign hashtag (from 20 Aug. 2016 to 1 Dec. 2016). This resulted in a dataset of 2,908,559 tweets, of which 225,883 were non-duplicated (i.e., some tweets were collected multiple times by the crawler).There were 3,230 user accounts participating during this period, with each user tweeting 70 times on average. As Figure 1 shows, a sizeable percentage of users were quite active at the height of the campaign, although there is clearly a number of users who only tweeted once or twice. More specifically, there were 1,232 users (or 38%) who tweeted at least 100 times, and on the other hand 1080 users (or 33%) who only tweeted two times or less. In addition, a tiny number of ‘power users’ (18 or 0.006%) tweeted more than 400 times during this period. Figure 1: Frequency distribution of #Mission22 tweets for each user in the datasetTo get a sense of what users were talking about during the campaign, we constructed a wordcloud out of the text data extracted from the tweets (see Figure 2). To provide more information and context, usernames (preceded with @) and hashtags (preceded with #) were included along with the words, providing a set of terms. As a result, the wordcloud also shows the user accounts and hashtags that were mentioned most often (note that #Mission22 was excluded from the data as it, by definition of the data collection process, has to occur in every tweet). In order to remove meaningless terms from the dataset we applied several text processing steps. First, all terms were converted to lowercase, such that “Veteran” and “veteran” are treated as the same term. Next, we applied a technique known as term frequency-inverse document frequency (tf-idf) to the tweet text data. Tf-idf effectively removes terms that occur so frequently that they provide no interesting information (e.g., the term “mission22”), and also terms that occur extremely infrequently. Finally, we removed English “stop words” from the text data, thereby eliminating common words such as “the” and “and”. Figure 2: Wordcloud of the #Mission22 tweet contentAs Figure 2 shows, the most frequent terms revolve around the campaign message and call-to-action for suicide awareness, including, for example, “day”, “veteran”, “support”, “push-ups”, “band”, “challenge”, “suicide”, “fight”, and “alone”. A number of user accounts are also frequently mentioned, which largely relate to the heavily retweeted users (discussed further below). Furthermore, alongside the central #mission22 hashtag, a number of other popular hashtags were in circulation during the campaign, including “#veteran”, “#americasmission”, “#22kill”, and “#22adayis22toomany”. Table 1 provides the top 50 most frequently occurring terms in decreasing order.Table 1: Top 50 words in the #Mission22 tweet content (decreasing order)1-1011-2021-3031-4041-50day@mrbernardedlong@uc_vetsnothingveteran#veteranbetter@kappasigmauceverysupporteverydaybelieve@ucthetachimissionpush-upschallengetodaytakehelp@sandratxassuicidehaulone#22kill@defensebaronveteransawarenessjustsay@the_usofightaccepted@piedmontlax#veterans@nbcnewsaloneptsdgoodweaknessbandvets22kwrong#nevertrumpcimmunity [sic]#americasmissionshoutoutgodwillA surprising finding of our study is that the vast majority of tweets are simply just retweets of other users. The number of retweets was 223,666, which accounts for about 99% of all tweets in the dataset. Even more surprising was that the vast majority of these retweets are from a single tweet. Indeed, 221,088 (or 98%) of all tweets in the dataset were retweets of the following tweet that was authored on 2 March 2015 by @SandraTXAS (see Figure 3). Clearly we can say that this tweet went ‘viral’ (Jenders et al) in the sense that it became frequently retweeted and gained an increasing amount of attention due to its cumulative popularity and visibility over time. Figure 3: #1 most retweeted #Mission22 tweet – @SandraTXAS (https://twitter.com/SandraTXAS)This highly retweeted or viral #Mission22 tweet provides a point of departure to examine what aspects of the tweet content influence the virality or popularity of #Mission22 tweets during the height of the campaign. To do this, we extracted the next nine most retweeted tweets from our dataset, providing an analysis of the “top 10” retweets (including the @SandraTXAS tweet above). Figure 4: #2 most retweeted - @mrbernarded (https://twitter.com/mrbernarded/status/776221040582295553)This tweet was retweeted 715 times in our dataset. Figure 5: #4 most retweeted - @Mission22 (https://twitter.com/Mission22/status/799872548863414272)This was retweeted 317 times in our dataset. Figure 6: #4 most retweeted - @UCThetaChi (https://twitter.com/UCThetaChi/status/784775641430384640)This was retweeted 180 times in our dataset. Figure 7: #5 most retweeted - @PamKeith2016 (https://twitter.com/PamKeith2016/status/782975576550305792)This was retweeted 121 times in our dataset. Figure 8: #6 most retweeted - @PiedmontLax (https://twitter.com/PiedmontLax/status/770749891698122752)This was retweeted 105 times in our dataset. Figure 9: #7 most retweeted - @PiedmontLax (https://twitter.com/PiedmontLax/status/771181070066692098) This was retweeted 78 times in our dataset. Figure 10: #8 most retweeted - @PatriotBrother (https://twitter.com/PatriotBrother/status/804387050728394752) This was retweeted 59 times in our dataset. Figure 11: #9 most retweeted - @alexgotayjr (https://twitter.com/alexgotayjr/status/787112936644849664) This was retweeted 49 times in our dataset. Figure 12: #10 most retweeted - @csjacobson89 (https://twitter.com/csjacobson89/status/772921614044233729) This was retweeted 45 times in our dataset.DiscussionThis article has provided the first “big data” analysis of the #Mission22 movement that went viral across multiple social media platforms in 2016. We began by arguing that Web 2.0 has ushered in profound changes to how people depict and construct identities that articulate with wider transformations in self and identity in conditions of late-modernity. The “confessional” quality of Web 2.0 means individuals and groups are presented with unprecedented opportunities to “mass self-depict” through new communication and Internet technologies. We suggest that the focus on how Web technologies are implicated in the formation of moral subjectivities is something that has been overlooked in the extant research on identity and Web 2.0 technologies.Filling this gap, we used the #Mission22 movement on Twitter as an empirical site to analyse how contemporary subjects construct and visually depict moral identities in online contexts. A central finding of our analysis of 225883 Twitter posts is that most engagement with #Mission22 was through retweeting. Our data show that retweets were by far the most popular way to interact and engage with the movement. In other words, most people were not producing original or new content in how they participated in the movement but were re-sharing – re-depicting – what others had shared. This finding highlights the importance of paying attention to the architectural affordances of social media platforms, in this case, the affordances of the ‘retweet’ button, and how they shape online identity practices and moral expression. We use moral expression here as a broad term to capture the different ways individuals and groups make moral evaluations based on a responsiveness to how people are faring and whether they are suffering or flourishing (Sayer). This approach provides an emic account of everyday morality and precludes, for example, wider philosophical debates about whether patriotism or nationalistic solidarity can be understood as moral values.The prominence of the retweet in driving the shape and nature of #Mission22 raises questions about the depth of moral engagement being communicated. Is the dominance of the retweet suggestive of a type of “moral slacktivism”? Like its online political equivalent, does the retweet highlight a shallow and cursory involvement with a cause or movement? Did online engagement translate to concrete moral actions such as making a donation to the cause or engaging in some other form of civic activity to draw attention to the movement? These questions are beyond the scope of this article but it is interesting to consider the link between the affordances of the platform, capacity for moral expression and how this translates to face-to-face moral action. Putting aside questions of depth, people are compelled not to ignore these posts, they move from “seeing” to “posting”, to taking action within the affordances of the architectural platform.What then is moving Twitter users to morally engage with this content? How did this movement go viral? What helped bust this movement out of the “long tail distribution” which characterises most movements – that is, few movements “take-off” and become durable within the congested attention economies of social media environments. The Top 10 most retweeted tweets provide powerful answers here. All of them feature highly emotive and affective visual depictions, either high impact photos and statements, or videos of people/groups doing pushups in solidarity together. The images and videos align affective, bodily and fitness practices with nationalistic and patriotic themes to produce a powerful and moving moral cocktail. The Top 50 words also capture the emotionally evocative use of moral language: words like: alone, fight, challenge, better, believe, good, wrong, god, help, mission, weakness and will.The emotional and embodied visual depictions that characterise the the Top 10 retweets and Top 50 words highlight how moral identity is not just a cerebral practice, but one that is fundamentally emotional and bodily. We do morality not just with our minds and heads but also with our bodies and our hearts. Part of the power of this movement, then, is the way it mobilises interest and involvement with the movement through a physical and embodied practice – doing push-ups. Visually depicting oneself doing push-ups online is a powerful display of morality identity. The “lay morality” being communicated is that not only are you somebody who cares about the flourishing and suffering of Others, you are also a fit, active and engaged citizen. And of course, the subject who actively takes responsibility for their health and well-being is highly valued in neoliberal risk contexts (Lupton).There is also a strong gendered dimensions to the visual depictions used in #Mission22. All of the Top 10 retweets feature images of men, mostly doing push-ups in groups. In the case of the second most popular retweet, it is two men in suits doing push-ups while three sexualised female singers “look-on” admiringly. Further analysis needs to be done to detail the gendered composition of movement participation, but it is interesting to speculate whether men were more likely to participate. The combination of demonstrating care for Other via a strong assertion of physical strength makes this a potentially more masculinised form of moral self-expression.Overall, Mission22 highlights how online self-work and cultivation can have a strong moral dimension. In Foucault’s language, the self-work involved in posting a video or image of yourself doing push-ups can be read as “an intensification of social relations”. It involves an ethics that is about self-creation through visual and textual depictions. Following the more pessimistic line of Bauman or Turkle, posting images of oneself doing push-ups might be seen as evidence of narcissism or a consumerist self-absorption. Rather than narcissism, we want to suggest that Mission22 highlights how a self-based moral practice – based on bodily, emotional and visual depictions – can extend to Others in an act of mutual care and exchange. Again Foucault helps clarify our argument: “the intensification of the concern for the self goes hand in hand with a valorisation of the Other”. What our work does, is show how this operates empirically on a large-scale in the new confessional contexts of Web 2.0 and its cultures of mass self-depiction. 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