Articles de revues : « L12D »

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O'Connor, Christopher, Philip Matsumura et Andres Campos. « The CheZ Binding Interface of CheAS Is Located in α-Helix E ». Journal of Bacteriology 191, n^o 18 (6 juillet 2009) : 5845–48. http://dx.doi.org/10.1128/jb.00294-09.

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ABSTRACT Specific CheA-short (CheAS) residues, L123 and L126, were identified as critical for CheZ binding. In the CheAS ′P1-CheZ nuclear magnetic resonance structure, these residues form an interaction surface on α-helix E in the ′P1 domain. Both L123 and L126 are buried in CheA-long (CheAL), providing an explanation for why CheAL fails to bind CheZ.

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Wang, Wenjing, Xupeng Zang, Yonglun Liu, Yunyi Liang, Gengyuan Cai, Zhenfang Wu et Zicong Li. « Dynamic miRNA Landscape Links Mammary Gland Development to the Regulation of Milk Protein Expression in Mice ». Animals 12, n^o 6 (14 mars 2022) : 727. http://dx.doi.org/10.3390/ani12060727.

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Mammary gland morphology varies considerably between pregnancy and lactation status, e.g., virgin to pregnant and lactation to weaning. Throughout these critical developmental phases, the mammary glands undergo remodeling to accommodate changes in milk production capacity, which is positively correlated with milk protein expression. The purpose of this study was to investigate the microRNA (miRNA) expression profiles in female ICR mice’s mammary glands at the virgin stage (V), day 16 of pregnancy (P16d), day 12 of lactation (L12d), day 1 of forced weaning (FW1d), and day 3 of forced weaning (FW3d), and to identify the miRNAs regulating milk protein gene expression. During the five stages of testing, 852 known miRNAs and 179 novel miRNAs were identified in the mammary glands. Based on their expression patterns, the identified miRNAs were grouped into 12 clusters. The expression pattern of cluster 1 miRNAs was opposite to that of milk protein genes in mammary glands in all five different stages. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that the predicted target genes of cluster 1 miRNAs were related to murine mammary gland development and lactation. Furthermore, fluorescence in situ hybridization (FISH) analysis revealed that the novel-mmu-miR424-5p, which belongs to the cluster 1 miRNAs, was expressed in murine mammary epithelial cells. The dual-luciferase reporter assay revealed that an important milk protein gene—β-casein (CSN2)—was regarded as one of the likely targets for the novel-mmu-miR424-5p. This study analyzed the expression patterns of miRNAs in murine mammary glands throughout five critical developmental stages, and discovered a novel miRNA involved in regulating the expression of CSN2. These findings contribute to an enhanced understanding of the developmental biology of mammary glands, providing guidelines for increasing lactation efficiency and milk quality.

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Ramirez, Celia, Lawrence C. Shimmin, C. Hunter Newton, Alastair T. Matheson et Patrick P. Dennis. « Structure and evolution of the L11, L1, L10, and L12 equivalent ribosomal proteins in eubacteria, archaebacteria, and eucaryotes ». Canadian Journal of Microbiology 35, n^o 1 (1 janvier 1989) : 234–44. http://dx.doi.org/10.1139/m89-036.

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The genes corresponding to the L11, L1, L10, and L12 equivalent ribosomal proteins (L11e, L1e, L10e, and L12e) of Escherichia coli have been cloned and sequenced from two widely divergent species of archaebacteria, Halobacterium cutirubrum and Sulfolobus solfataricus, and the L10 and four different L12 genes have been cloned and sequenced from the eucaryote Saccharomyces cerevisiae. Alignments between the deduced amino acid sequences of these proteins and to other available homologous proteins of eubacteria and eucaryotes have been made. The data suggest that the archaebacteria are a distinct coherent phylogenetic group. Alignment of the proline-rich L11e proteins reveals that the N-terminal region, believed to be responsible for interaction with release factor 1, is the most highly conserved region and that there is specific conservation of most of the proline residues, which may be important in maintaining the highly elongated structure of the molecule. Although L11 is the most highly methylated protein in the E. coli ribosome, the sites of methylation are not conserved in the archaebacterial L11e proteins. The L1e proteins of eubacteria and archaebacteria show two regions of very high similarity near the center and the carboxy termini of the proteins. The L10e proteins of all kingdoms are colinear and contain approximately three fourths of an L12e protein fused to their carboxy terminus, although much of this fusion has been lost in the truncated eubacterial protein. The archaebacterial and eucaryotic L12e proteins are colinear, whereas the eubacterial protein has suffered a rearrangement through what appear to be gene fusion events. Within the L12e derived region of the L10e proteins there exists a repeated module of 26 amino acids, present in two copies in eucaryotes, three in archaebacteria, and one in eubacteria. This modular sequence is apparently also present in the L12e proteins of all kingdoms and may play a role in L12e dimerization, L10e–L12e complex formation, and the function of the L10e–L12e complex in translation.Key words: translation, ribosome.

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Stults, John T., Patrick R. Griffin, David D. Lesikar, Asha Naidu, Barbara Moffat et Bradley J. Benson. « Lung surfactant protein SP-C from human, bovine, and canine sources contains palmityl cysteine thioester linkages ». American Journal of Physiology-Lung Cellular and Molecular Physiology 262, n^o 3 (1 mars 1992) : 1. http://dx.doi.org/10.1152/ajplung.1992.262.3.1-a.

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Pages L118B–L125: John T. Stults, Patrick R. Griffin, David D. Lesikar, Asha Naidu, Barbara Moffat, and Bradley J. Benson. “Lung surfactant protein SP-C from human, bovine, and canine sources contains palmityl cysteine thioester linkages.” Page L124: received and accepted dates were inadvertently omitted. They should read “Received 4 October 1990; accepted in final form 11 February 1991.”

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Stults, John T., Patrick R. Griffin, David D. Lesikar, Asha Naidu, Barbara Moffat et Bradley J. Benson. « Lung surfactant protein SP-C from human, bovine, and canine sources contains palmityl cysteine thioester linkages ». American Journal of Physiology-Lung Cellular and Molecular Physiology 262, n^o 6 (1 juin 1992) : 1. http://dx.doi.org/10.1152/ajplung.1992.262.6.1-b.

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Pages L118B–L125: John T. Stults, Patrick R. Griffin, David D. Lesikar, Asha Naidu, Barbara Moffat, and Bradley J. Benson. “Lung surfactant protein SP-C from human, bovine, and canine sources contains palmityl cysteine thioester linkages.” Page L124: received and accepted dates were inadvertently omitted. They should read “Received 4 October 1990; accepted in final form 11 February 1991.”

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Shimmin, Lawrence C., C. Hunter Newton, Celia Ramirez, Janet Yee, Willa Lee Downing, Andrea Louie, Alastair T. Matheson et Patrick P. Dennis. « Organization of genes encoding the L11, L1, L10, and L12 equivalent ribosomal proteins in eubacteria, archaebacteria, and eucaryotes ». Canadian Journal of Microbiology 35, n^o 1 (1 janvier 1989) : 164–70. http://dx.doi.org/10.1139/m89-025.

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Archaebacterial and eucaryotic cytoplasmic ribosomes contain proteins equivalent to the L11, L1, L10, and L12 proteins of the eubacterium Escherichia coli. In E. coli the genes encoding these ribosomal proteins are clustered, cotranscribed, and autogenously regulated at the level of mRNA translation. Genomic restriction fragments encoding the L11e, L1e, L10e, and L12e (equivalent) proteins from two divergent archaebacteria, Halobacterium cutirubrum and Sulfolobus solfataricus, and the L10e and L12e proteins from the eucaryote Saccharomyces cerevisiae have been cloned, sequenced, and analyzed. In the archaebacteria, as in eubacteria, the four genes are clustered and the L11e, L1e, L 10e, and L12e order is maintained. The transcription pattern of the H. cutirubrum cluster is different from the E. coli pattern and the flanking genes on either side of the tetragenic clusters in E. coli, H. cutirubrum, and Sulfolobus solfataricus are all unrelated to each other. In the eucaryote Saccharomyces cerevisiae there is a single L10e gene and four separate L12e genes that are designated L12eIA, L12eIB, L12eIIA, and L12eIIB. These five genes are not closely linked and each is transcribed as a monocistronic mRNA; the L10e, L12eIA, L12eIB, and the L12eIIA genes are contiguous and uninterrupted, whereas the L12eIIB gene is interrupted by a 301 nucleotide long intron located between codons 38 and 39.Key words: archaebacteria, ribosome, Halobacterium, Sulfolobus.

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Khanzada, Nimra, Owais Iqbal, Ambrin Baby, Sumbal Zaman, Mehvish Tofique et Asad Rajput. « EVALUATION OF CHICKPEA GERMPLASM FOR RELATIVE RESISTANCE OR SUSCEPTIBILITY AGAINST FUSARIUM WILT AND ASCOCHYTA BLIGHT UNDER FIELD CONDITIONS ». Plant Protection 6, n^o 2 (23 août 2022) : 121–32. http://dx.doi.org/10.33804/pp.006.02.4234.

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Recognition and use of resistant sources against pests and diseases are an integral element of a genetic improvement program. For this purpose, an evaluation of chickpea cultivars (24 advanced lines and 6 commercial varieties) was undertaken under field conditions. Three types of disease responses based on a disease rating scale of 1-9 were observed i.e. resistant, moderately resistant, and susceptible. It was noticed that among 30 cultivars, none was highly resistant and asymptomatic or highly susceptible to both diseases. It was remarkably noticed that in the case of Fusarium wilt, all of the chickpea genotypes except one which performed better was categorized as resistant. Contrarily, L124 was scored as moderately susceptible. All the commercial varieties NUT (2018-19), DG-92, Rabat, Black gram, Benezir, and Synyasi were susceptible to Fusarium wilt. In the case of Ascochyta blight, all the germplasm exhibited resistant reactions except one (L124). Out of the six commercial varieties, Black gram and Benezir exhibited resistant reactions. Fusarium wilt and Ascochyta blight gradually increased with time after each observation. Fusarium wilt disease index in the month of March, was significantly higher on commercial varieties, including NUT (2018-19), DG-92, Rabat, Benezir, and Synyasi, ranging between 57.4-61.7% followed by cv. Black gram with a disease index of 53.7%. All the advanced lines had a low disease index as compared to commercial varieties. Similarly, in the month of March, the disease index of Ascochyta blight was lowest on L102 (38.9%) and the highest on Nut-2018-19 (59.3%) followed by Rabat and Synyasi. Significantly maximum 1000 grain weight was recorded in DG-92, L10, and L119, ranging from 304.3-305.3 g. In terms of grain yield/hectare, L117, L124, NUT (2018-19) and Black gram produced a significantly maximum yield (2916.7-2868.1 kg/ha) followed by Rabat (2638.9 kg/ha) and Synyasi (2520.8 kg/ha) whereas, the lowest yield was recorded in L121. The disease severity of both diseases was positively correlated with 100-grain weight as well as with grain yield. The study revealed the availability of resistant germplasm against two important diseases (Fusarium wilt and Ascochyta blight) which may be exploited in the breeding program for the development of disease-resistant cultivars and may be incorporated with high-yielding cultivars which are clearly evident in the present study.

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Ge, Xueliang, Chandra Sekhar Mandava, Christoffer Lind, Johan Åqvist et Suparna Sanyal. « Complementary charge-based interaction between the ribosomal-stalk protein L7/12 and IF2 is the key to rapid subunit association ». Proceedings of the National Academy of Sciences 115, n^o 18 (23 avril 2018) : 4649–54. http://dx.doi.org/10.1073/pnas.1802001115.

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The interaction between the ribosomal-stalk protein L7/12 (L12) and initiation factor 2 (IF2) is essential for rapid subunit association, but the underlying mechanism is unknown. Here, we have characterized the L12–IF2 interaction on Escherichia coli ribosomes using site-directed mutagenesis, fast kinetics, and molecular dynamics (MD) simulations. Fifteen individual point mutations were introduced into the C-terminal domain of L12 (L12-CTD) at helices 4 and 5, which constitute the common interaction site for translational GTPases. In parallel, 15 point mutations were also introduced into IF2 between the G4 and G5 motifs, which we hypothesized as the potential L12 interaction sites. The L12 and IF2 mutants were tested in ribosomal subunit association assay in a stopped-flow instrument. Those amino acids that caused defective subunit association upon substitution were identified as the molecular determinants of L12–IF2 interaction. Further, MD simulations of IF2 docked onto the L12-CTD pinpointed the exact interacting partners—all of which were positively charged on L12 and negatively charged on IF2, connected by salt bridges. Lastly, we tested two pairs of charge-reversed mutants of L12 and IF2, which significantly restored the yield and the rate of formation of the 70S initiation complex. We conclude that complementary charge-based interaction between L12-CTD and IF2 is the key for fast subunit association. Considering the homology of the G domain, similar mechanisms may apply for L12 interactions with other translational GTPases.

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Lin, Tz-Feng, Wei-Chieh Wang, Xin-Yu Zeng, Yi-Xian Lu et Pei-Jung Shih. « Preparation, Structural Characterization of Anti-Cancer Drugs-Mediated Self-Assembly from the Pluronic Copolymers through Synchrotron SAXS Investigation ». Materials 15, n^o 15 (5 août 2022) : 5387. http://dx.doi.org/10.3390/ma15155387.

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Chemotherapy drugs are mainly administered via intravenous injection or oral administration in a very a high dosage. If there is a targeted drug vehicle which can be deployed on the tumor, the medical treatment is specific and precise. Binary mixing of biocompatible Pluronic® F127 and Pluronic® L121 was used in this study for a drug carrier of pluronic biomedical hydrogels (PBHs). Based on the same PBH ingredients, the addition of fluorouracil (5-FU) was separated in three ways when it was incorporated with pluronics: F127-L121-(5-FU), F127-(5-FU), and L121-(5-FU). Small angle X-ray scattering experiments were performed to uncover the self-assembled structures of the PBHs. Meanwhile, the expected micelle and lamellar structural changes affected by the distribution of 5-FU were discussed with respect to the corresponding drug release monitoring. PBH-all with the mixing method of F127-L121-(5-FU) has the fastest drug release rate owing to the undulated amphiphilic boundary. In contrast, PBH-2 with the mixing method of L121-(5-FU) has a prolonged drug release rate at 67% for one month of the continuous drug release experiment because the flat lamellar amphiphilic boundary of PBH-2 drags the migration of 5-FU from the hydrophobic core. Therefore, the PBHs developed in the study possess great potential for targeted delivery and successfully served as a microenvironment model to elucidate the diffusion pathway of 5-FU.

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Han, Chang-Suk, Kyung-Wan Koo et Dong-Cheol Oh. « A Study on the Precipitation Behavior of Carbide Particle in L12-type Intermetallic Compound Ni3Al ». Korean Journal of Materials Research 16, n^o 4 (27 avril 2006) : 241–47. http://dx.doi.org/10.3740/mrsk.2006.16.4.241.

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Masni, Tiara Elsita, Tetty Marta Linda et Bernadeta Leni Fibriarti. « STUDI PERTUMBUHAN DAN DEGRADASI FENOL OLEH KULTUR TUNGGAL AKTINOMISETES DARI TANAH GAMBUT ». Al-Kauniyah : Jurnal Biologi 13, n^o 1 (30 avril 2020) : 95–104. http://dx.doi.org/10.15408/kauniyah.v13i1.12854.

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AbstrakFenol adalah senyawa organik yang bersifat toksik dan larut dalam air, sehingga mudah menimbulkan pencemaran pada perairan dan menurunkan kualitas air. Penelitian ini bertujuan untuk melihat potensi tiga isolat aktinomisetes asal tanah gambut Riau dalam Minimal Salt Medium yang mengandung fenol pada konsentrasi 0 ppm, 200 ppm, 400 ppm, dan 600 ppm serta mengetahui kemampuan aktinomisetes dalam mendegradasi fenol pada konsentrasi 600 ppm menggunakan metode folin ciocalteau. Potensi pertumbuhan isolat L121, L18, L11 menunjukkan total populasi tidak berbeda nyata dengan penambahan 400 ppm dan 600 ppm fenol, tetapi berbeda nyata terhadap 0 ppm dan 200 ppm fenol. Potensi pertumbuhan tertinggi terdapat pada isolat L121 dan terendah pada isolat L11. Kemampuan degradasi fenol oleh masing-masing isolat adalah L121 sebesar 570,80 ppm (95%), L18 sebesar 218,85 ppm (36%) dan L11 sebesar 97,21 ppm (16%) dari konsentrasi fenol awal 600 ppm pada Minimal Salt Medium. Isolat aktinomisetes ini berpotensi dikembangkan untuk penanggulangan pencemaran di lingkungan.Abstract Phenol is an organic compound is toxic and easily soluble in water so easy to cause pollution in a waters such as water quality degradation. The aim of this research is to see the potential of three isolates of actinomycetes from Riau peat soil in Minimal Salt Medium containing phenol concentration 0 ppm, 200 ppm, 400 ppm and 600 ppm and to know the ability of actinomycetes in degradation of phenol at the concentration of 600 ppm using folin ciocalteu. The growth potential of L121, L18, L11 isolates showed the total population was not significantly different with the addition of 400 ppm and 600 ppm of phenol but significantly different from 0 ppm and 200 ppm of phenol. The highest growth potential was found in L121 isolate and lowest in L11 isolate. The degradation ability of phenols by each isolate was L121 570.80 ppm (95%), L18 218.85 ppm (36%) and L11 was able to degrade phenol 97.21 ppm (16%) from the initial phenol concentration of 600 ppm at Minimum Salt Medium.These actinomycetes have the potential to be developed for the overcome of pollution in the environment.

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Kozakai, Takao, C. Aoki, Tomokazu Moritani et Minoru Doi. « Precipitation of Gamma-Prime and Gamma-Double Prime Coherent Phases in Three-Phase Ni-V-Si Alloys ». Materials Science Forum 539-543 (mars 2007) : 3036–41. http://dx.doi.org/10.4028/www.scientific.net/msf.539-543.3036.

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Phase separation of γ (A1) supersaturated solid solution into A1, γ’ (L12) and γ” (D022) phases was investigated in two Ni-rich Ni-V-Si ternary alloys by means of transmission electron microscopy. When the alloys are annealed at 1073K, two different sequences of the phase separation are observed, depending on the chemical composition of the alloy: In Ni-17.0at%V-6.9at%Si alloy (A) at the D022 corner of three-phase field, first many D022 particles precipitate aligning along the <110> direction of the matrix and the so-called chessboard pattern is observed, followed by the formation of L12 phase at the interface between D022 and A1 phases. In Ni-12.1at%V-11.3at%Si alloy (B) at the L12 corner of the Gibbs triangle, cuboidal L12 particles precipitate arranging along the <100> direction, and then D022 phase is formed. As the phase separation proceeds, a selective growth/formation of the third phase (L12 in the alloy A, D022 in the alloy B) occurs: In the alloy A, L12 phase grows into D022 particle inside along the diagonal direction of D022 cube which is parallel to the a-axis of D022 tetragonal phase. In the alloy B, D022 forms on the {100} cube face of cuboidal L12 particle, arranging the c-axis of D022 perpendicular to the {100} cube face of L12 phase. As a result of such a selective growth/formation, the first phase D022/L12 is split off into two particles, which results in the formation of laminated structure consisting of D022 and L12 phases. The selective growth/formation is considered to occur so as to maintain the less elastic strain state.

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Mangla, Anil, Goutam Deo et Pankaj A. Apte. « Cooperative freezing of the L12 ordered domains at the critical cooling temperature of Ni₃Fe alloy ». Journal of Statistical Mechanics : Theory and Experiment 2022, n^o 9 (1 septembre 2022) : 093204. http://dx.doi.org/10.1088/1742-5468/ac8c89.

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Abstract It is well known that Ni3Fe transforms from a disordered solid solution to an ordered intermetallic with L12 superstructure when the alloy is cooled slowly. Here we elucidate the underlying cooperative phenomenon and the atomistic mechanism of this ordering process based on simulations using embedded atom potentials. As the simulated alloy is cooled from the disordered state to the critical cooling temperature (T c), Ni atoms with L12 order [denoted as Ni(L12 ⩾ 1) atoms] increase significantly along with Ni atoms having the least deviation from L12 local order (denoted as Ni([IP]3) atoms). The ordering (up to T c) occurs predominantly through random increase in Ni(L12 ⩾ 1) atoms throughout the system, as indicated by absence of long-range order. At T c, L12 ordered domains formed by Ni(L12 ⩾ 1) atoms ‘freeze’, i.e. these domains, collectively, achieve a threshold strength against thermal fluctuations. This is indicated by (i) dissipation of large-scale fluctuations of Ni(L12 ⩾ 1) atoms at T c and (ii) the growth of the L12 domains through propagation (at the expense of atoms with non-L12 local environment) as the alloy is cooled below T c. The stability threshold of the L12 ordered domains at T c is qualitatively consistent with (i) the critical slowing down, i.e. a significant increase in annealing time (to about 41 days) at 497 °C close to T c (∼500 °C) and (ii) sharp changes in bulk properties (due to loss of stability of the domains) when the alloy is heated across T c to about 550 °C. Further, the experimental long-range order parameter values as a function of reduced temperature are in reasonable agreement with the corresponding values of the simulated alloys. The contribution of Ni([IP]3) atoms to ordering in the actual alloy is potentially significant since such atoms together with nearest neighbours constitute about 75% of the total atoms in the simulated alloys at T c.

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Tegopoulos, Konstantinos, Odysseas Sotirios Stergiou, Despoina Eugenia Kiousi, Margaritis Tsifintaris, Ellie Koletsou, Aristotelis C. Papageorgiou, Anthoula A. Argyri, Nikos Chorianopoulos, Alex Galanis et Petros Kolovos. « Genomic and Phylogenetic Analysis of Lactiplantibacillus plantarum L125, and Evaluation of Its Anti-Proliferative and Cytotoxic Activity in Cancer Cells ». Biomedicines 9, n^o 11 (19 novembre 2021) : 1718. http://dx.doi.org/10.3390/biomedicines9111718.

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Lactiplantibacillus plantarum is a diverse species that includes nomadic strains isolated from a variety of environmental niches. Several L. plantarum strains are being incorporated in fermented foodstuffs as starter cultures, while some of them have also been characterized as probiotics. In this study, we present the draft genome sequence of L. plantarum L125, a potential probiotic strain presenting biotechnological interest, originally isolated from a traditional fermented meat product. Phylogenetic and comparative genomic analysis with other potential probiotic L. plantarum strains were performed to determine its evolutionary relationships. Furthermore, we located genes involved in the probiotic phenotype by whole genome annotation. Indeed, genes coding for proteins mediating host–microbe interactions and bile salt, heat and cold stress tolerance were identified. Concerning the potential health-promoting attributes of the novel strain, we determined that L. plantarum L125 carries an incomplete plantaricin gene cluster, in agreement with previous in vitro findings, where no bacteriocin-like activity was detected. Moreover, we showed that cell-free culture supernatant (CFCS) of L. plantarum L125 exerts anti-proliferative, anti-clonogenic and anti-migration activity against the human colon adenocarcinoma cell line, HT-29. Conclusively, L. plantarum L125 presents desirable probiotic traits. Future studies will elucidate further its biological and health-related properties.

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Benameur, T., et A. R. Yavari. « Disordering and amorphization of L12-type alloys by mechanical attrition ». Journal of Materials Research 7, n^o 11 (novembre 1992) : 2971–77. http://dx.doi.org/10.1557/jmr.1992.2971.

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X-ray diffraction patterns obtained during the grinding of Ni3Ge and Ni3Al alloys which at equilibrium exhibit the L12 ordered fcc structures show the emergence of a nanocrystalline structure and transformation to the disordered fcc form but little amorphization. Furthermore, the non-L12 Al2Pt alloy which also has a more strongly negative heat of mixing is easier to amorphize than the Ni3Ge and Ni3Al with L12 superstructure. This is in contrast to the Zr3Al compound (also L12-type) for which a short milling time is sufficient for obtaining complete amorphization. Variations in the aptitudes toward amorphization of the three L12-type alloys under ball-milling conditions are attributed in part to the differences in the lattice stability terms of their disordered fcc phases.

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Hayashi, Eiki, Satoru Kobayashi, Kazuhisa Sato, Toyohiko J. Konno, Yasuyuki Kaneno et Takayuki Takasugi. « The Effect of Ti Addition on Phase Equilibria among Ni (A1), Ni₃Al (L1₂) and Ni₃V (D0₂₂) Phases ». Materials Science Forum 654-656 (juin 2010) : 432–35. http://dx.doi.org/10.4028/www.scientific.net/msf.654-656.432.

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The effect of Ti addition on phase equilibria among Ni (A1), Ni3Al (L12) and Ni3V (D022) phases at 950 °C was investigated through TEM/EDS analysis on heat-treated alloys. The three-phase coexisting region of A1 + L12 + D022 was found to exist around the composition of Ni-4Al-19V (at. %) in the Ni-Al-V ternary system. With addition of Ti to the ternary system the three-phase coexisting region was shifted to the Ni-rich side. Ti partitioned most into the L12 phase and least into the A1 phase. These results suggest that the addition of Ti stabilizes the L12 and D022 phases against A1 phase, and raises the temperature of eutectoid reaction in the Ni3Al-Ni3V pseudo-binary system: A1 → L12 + D022.

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Kozakai, Takao, Daisuke Sakurai et Minoru Doi. « Substitution Effect of the Third Element X on the Morphology of A1/L1₂/D0₂₂ Three-Phase Microstructure in Ni-V-X (X=Si, Al) Alloy ». Solid State Phenomena 172-174 (juin 2011) : 236–41. http://dx.doi.org/10.4028/www.scientific.net/ssp.172-174.236.

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Morphology of phase-separated microstructure consisting of cubic disordered A1 and ordered L12 phases and tetragonal ordered D022 phase in Ni-V-X (X=Al, Si) alloys has been investigated by transmission electron microscopy. Ternary Ni-V-Al alloy showed the lamellar structure of D022 phase with spherical L12 particles, while Ni-V-Si ternary alloy indicated the anisotropic microstructure consisting of plate- or diamond-shaped D022 and plate-shaped L12 phases. When a part of element Al is substituted by Si, the morphology of three-phase microstructure (A1/L12/D022) varied depending on the amount of element substitution. When Ni-16.1V-4.0Al-2.8Si (at.%) alloy was aged at 1173K for long time, D022 plate-like particle aligned along <110> direction and L12 particles formed between them in the shape of sphere. In the case of Ni-16.6V-2.8Al-4.0Si (at%) alloy more substituted for Al by Si, cuboidal L12 phases were first formed aligning along <100> direction and then plate-shaped D022 phases precipitated along the <100> direction in the channel of cuboidal L12 particles. Such morphological changes with the substitution of the third elements X by Al or Si were able to be explained based on lattice mismatch.

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Watanabe, Yoshimi. « Review, Role of L12 Modified (Al1−xMex)3Ti Intermetallic Compounds on Heterogeneous Nucleation of Alpha Aluminum Grains ». MATEC Web of Conferences 326 (2020) : 06008. http://dx.doi.org/10.1051/matecconf/202032606008.

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Al3Ti intermetallic compound with the tetragonal D022 structure undergoes a phase transformation to the high-symmetry L12 cubic structure by addition of third elements, Me. The lattice constants of some L12 modified (Al1−xMex)3Ti intermetallic compounds are closed to that of alpha aluminum. Therefore, it is expected that the addition of L12 modified (Al1−xMex)3Ti intermetallic compound particles show good grain refining performance of cast aluminum. In this paper, our recent results on novel refines containing heterogeneous nucleation site particles of L12 modified (Al1−xMex)3Ti intermetallic compounds have been reviewed.

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Ribeiro, Luciana A., Vasco Azevedo, Yves Le Loir, Sergio C. Oliveira, Yakhya Dieye, Jean-Christophe Piard, Alexandra Gruss et Philippe Langella. « Production and Targeting of the Brucella abortus Antigen L7/L12 in Lactococcus lactis : a First Step towards Food-Grade Live Vaccines against Brucellosis ». Applied and Environmental Microbiology 68, n^o 2 (février 2002) : 910–16. http://dx.doi.org/10.1128/aem.68.2.910-916.2002.

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ABSTRACT Brucella abortus is a facultative intracellular gram-negative bacterial pathogen that infects humans and animals by entry mainly through the digestive tract. B. abortus causes abortion in pregnant cattle and undulant fever in humans. The immunogenic B. abortus ribosomal protein L7/L12 is a promising candidate antigen for the development of oral live vaccines against brucellosis, using food-grade lactic acid bacteria (LAB) as a carrier. The L7/L12 gene was expressed in Lactococcus lactis, the model LAB, under the nisin-inducible promoter. Using different signals, L7/L12 was produced in cytoplasmic, cell-wall-anchored, and secreted forms. Cytoplasmic production of L7/L12 gave a low yield, estimated at 0.5 mg/liter. Interestingly, a secretable form of this normally cytoplasmic protein via fusion with a signal peptide resulted in increased yield of L7/L12 to 3 mg/liter; secretion efficiency (SE) was 35%. A fusion between the mature moiety of the staphylococcal nuclease (Nuc) and L7/L12 further increased yield to 8 mg/liter. Fusion with a synthetic propeptide (LEISSTCDA) previously described as an enhancer for heterologous protein secretion in L. lactis (Y. Le Loir, A. Gruss, S. D. Ehrlich, and P. Langella, J. Bacteriol. 180:1895-1903, 1998) raised the yield to 8 mg/liter and SE to 50%. A surface-anchored L7/L12 form in L. lactis was obtained by fusing the cell wall anchor of Streptococcus pyogenes M6 protein to the C-terminal end of L7/L12. The fusions described allow the production and targeting of L7/L12 in three different locations in L. lactis. This is the first example of a B. abortus antigen produced in a food-grade bacterium and opens new perspectives for alternative vaccine strategies against brucellosis.

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Dong, Wei Ping, et Zheng Chen. « Computer Simulation of the Early Stage of Precipitation of L1₂-Ni₃(Al,V) Using Microscopic Phase Field Method ». Materials Science Forum 817 (avril 2015) : 809–15. http://dx.doi.org/10.4028/www.scientific.net/msf.817.809.

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Based on the phase field theory, the long-range order (LRO) parameter related interatomic potentials equations were utilized to calculate the interatomic potentials of L10-Ni3(Al,V), L12-Ni3Al and L12-Ni3(Al,V) phases varying with temperature and concentrations. Using these potentials, the simulated microstructure evolution and the order parameter with the time of Ni75Al20V5 ternary alloy are simulated at temperature 1000K during the early stage of the precipitation process in this research. Results testify that the precipitation sequence during the early stage of Ni75Al20V5 alloy is the disordered phase →L10 pre-precipitation phase →L12 equilibrium phase. Firstly, the nonstoichiometric L10 pre-precipitation phase formed by congruent ordering precipitation mechanism; secondly, the nonstoichiometric L12 phase formed by transforming from L10 phase; thirdly, the stoichiometric equilibrium L12 phase formed by spinodal decomposition precipitation mechanism. It is discovered that the precipitation mechanism (congruent ordering+ spinodal decomposition) process was closely related to free energy and interatomic potentials: L10 pre-precipitation phase’s free energies are higher and interatomic potentials are smaller than those of L12 equilibrium phase.

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Kenedy, Gurumayum Robert, Korir Rosemary Chemeli et Wei-Chun Cheng. « The Observation of Cellular Precipitation in an Ni36Co18Cr20Fe19Al7 High-Entropy Alloy after Quenching and Annealing ». Materials 15, n^o 19 (23 septembre 2022) : 6613. http://dx.doi.org/10.3390/ma15196613.

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High-entropy alloys (HEAs) comprise a minimum of five major elements. These alloys show some special characteristics, such as excellent mechanical and high temperature properties. The development of the HEAs requires a knowledge of phase transformations during alloy making procedures. The phase transformations of an Ni36Co18Cr20Fe19Al7 HEA were studied in this research. The alloy underwent hot forging, cold rolling, annealing at and quenching from 1323 K, and isothermal holding at 873 K. The alloy is a single face-centered cubic (FCC) phase in the as-quenched condition. After annealing at 873 K, not only fine coherent L12 particles precipitated homogeneously in the FCC matrix, but lamellae of FCC and L12 phases also developed from the grain boundaries. Both lamellar FCC and L12 grains have a cubic-on-cubic orientation relationship (OR). The composition of the lamellar L12 phase is Ni60Co8Cr6Fe6Al20, and that of the lamellar FCC phase is Ni31Co15Cr28Fe21Al4. Cellular precipitation occurs in the HEA, and the high-temperature FCC (γ) transforms to a lamella of low-temperature FCC (γ1), and an L12 phase, i.e., γ → γ1+L12.

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Balagurov, Anatoly M., Nataliya Yu Samoylova, Ivan A. Bobrikov, Sergey V. Sumnikov et Igor S. Golovin. « The first- and second-order isothermal phase transitions in Fe3Ga-type compounds ». Acta Crystallographica Section B Structural Science, Crystal Engineering and Materials 75, n^o 6 (9 novembre 2019) : 1024–33. http://dx.doi.org/10.1107/s2052520619013106.

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Structural features and kinetics of the transition between ordered metastable b.c.c.-derived D03 and equilibrium f.c.c.-derived L12 phases of Fe–xGa alloys (x = 27.2% and 28.0%) have been analyzed by in situ real-time neutron diffraction during isothermal annealing in the temperature range 405–470°C. It has been revealed that the transition proceeds with alternation of the first- and second-order phase transformations according to a D03 → A2 → A1 → L12 scheme, where A2 and A1 are disordered b.c.c. and f.c.c. structures. Deformations of the crystal lattice that arise due to these transitions are determined. The kinetics of the L12 phase nucleation and growth were analyzed in the frame of the Johnson–Mehl–Avrami–Kolmogorov (JMAK) model; however, only the early stage of the D03 → L12 transition is well described by the JMAK equation. The value of the Avrami exponent corresponds to the constant growth rate of the new L12 phase and decreasing nucleation rate in the Fe–27.2Ga alloy and indicates the presence of pre-existing nucleation centres of the L12 phase in the Fe–28.0Ga alloy.

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Lin, Yuan, Yan Li, Ningyu Zhu, Yanxing Han, Wei Jiang, Yanchang Wang, Shuyi Si et Jiandong Jiang. « The Antituberculosis Antibiotic Capreomycin Inhibits Protein Synthesis by Disrupting Interaction between Ribosomal Proteins L12 and L10 ». Antimicrobial Agents and Chemotherapy 58, n^o 4 (21 janvier 2014) : 2038–44. http://dx.doi.org/10.1128/aac.02394-13.

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ABSTRACTCapreomycin is a second-line drug for multiple-drug-resistant tuberculosis (TB). However, with increased use in clinics, the therapeutic efficiency of capreomycin is decreasing. To better understand TB resistance to capreomycin, we have done research to identify the molecular target of capreomycin.Mycobacterium tuberculosisribosomal proteins L12 and L10 interact with each other and constitute the stalk of the 50S ribosomal subunit, which recruits initiation and elongation factors during translation. Hence, the L12-L10 interaction is considered to be essential for ribosomal function and protein synthesis. Here we provide evidence showing that capreomycin inhibits the L12-L10 interaction by using an established L12-L10 interaction assay. Overexpression of L12 and/or L10 inM. smegmatis, a species close toM. tuberculosis, increases the MIC of capreomycin. Moreover, both elongation factor G-dependent GTPase activity and ribosome-mediated protein synthesis are inhibited by capreomycin. When protein synthesis was blocked with thiostrepton, however, the bactericidal activity of capreomycin was restrained. All of these results suggest that capreomycin seems to inhibit TB by interrupting the L12-L10 interaction. This finding might provide novel clues for anti-TB drug discovery.

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Loeb, D. D., R. W. Padgett, S. C. Hardies, W. R. Shehee, M. B. Comer, M. H. Edgell et C. A. Hutchison. « The sequence of a large L1Md element reveals a tandemly repeated 5' end and several features found in retrotransposons ». Molecular and Cellular Biology 6, n^o 1 (janvier 1986) : 168–82. http://dx.doi.org/10.1128/mcb.6.1.168-182.1986.

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The complete nucleotide sequence of a 6,851-base pair (bp) member of the L1Md repetitive family from a selected random isolate of the BALB/c mouse genome is reported here. Five kilobases of the element contains two overlapping reading frames of 1,137 and 3,900 bp. The entire 3,900-bp frame and the 3' 600 bp of the 1,137-bp frame, when compared with a composite consensus primate L1 sequence, show a ratio of replacement to silent site differences characteristic of protein coding sequences. This more closely defines the protein coding capacity of this repetitive family, which was previously shown to possess a large open reading frame of undetermined extent. The relative organization of the 1,137- and 3,900-bp reading frames, which overlap by 14 bp, bears resemblance to protein-coding, mobile genetic elements. Homology can be found between the amino acid sequence of the 3,900-bp frame and selected domains of several reverse transcriptases. The 5' ends of the two L1Md elements described in this report have multiple copies, 4 2/3 copies and 1 2/3 copy, of a 208-bp direct tandem repeat. The sequence of this 208-bp element differs from the sequence of a previously defined 5' end for an L1Md element, indicating that there are at least two different 5' end motifs for L1Md.

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Loeb, D. D., R. W. Padgett, S. C. Hardies, W. R. Shehee, M. B. Comer, M. H. Edgell et C. A. Hutchison. « The sequence of a large L1Md element reveals a tandemly repeated 5' end and several features found in retrotransposons. » Molecular and Cellular Biology 6, n^o 1 (janvier 1986) : 168–82. http://dx.doi.org/10.1128/mcb.6.1.168.

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The complete nucleotide sequence of a 6,851-base pair (bp) member of the L1Md repetitive family from a selected random isolate of the BALB/c mouse genome is reported here. Five kilobases of the element contains two overlapping reading frames of 1,137 and 3,900 bp. The entire 3,900-bp frame and the 3' 600 bp of the 1,137-bp frame, when compared with a composite consensus primate L1 sequence, show a ratio of replacement to silent site differences characteristic of protein coding sequences. This more closely defines the protein coding capacity of this repetitive family, which was previously shown to possess a large open reading frame of undetermined extent. The relative organization of the 1,137- and 3,900-bp reading frames, which overlap by 14 bp, bears resemblance to protein-coding, mobile genetic elements. Homology can be found between the amino acid sequence of the 3,900-bp frame and selected domains of several reverse transcriptases. The 5' ends of the two L1Md elements described in this report have multiple copies, 4 2/3 copies and 1 2/3 copy, of a 208-bp direct tandem repeat. The sequence of this 208-bp element differs from the sequence of a previously defined 5' end for an L1Md element, indicating that there are at least two different 5' end motifs for L1Md.

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Yang, Ya-Wun, et Chih-Feng Chang. « Induction of differentiation of hematopoietic cells by the vaccine adjuvants (75.10) ». Journal of Immunology 188, n^o 1_Supplement (1 mai 2012) : 75.10. http://dx.doi.org/10.4049/jimmunol.188.supp.75.10.

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Abstract Vaccine adjuvants have been used to enhance the immune responses against the co-administered antigens. The mechanisms of action by the adjuvants however remain poorly understood. We attempt to investigate in this study the effect of vaccine adjuvants on differentiation of the hematopoietic system. C57BL/6J mice were vaccinated with ovalbumin (OVA) in L121-adjuvant, an emulsion adjuvant consists of Pluronic L121 that previously shown to stimulate cytotoxic lymphocyte (CTL) effect. Cells were isolated from bone marrow and thymus after vaccination, followed by flow cytometric analysis. Our data showed that treatment of animals with L121-adjuvant skewed the differentiation of hematopoietic cells, resulting in a significant increase of the CD11b+ population at the expense of lineage (lin)+ cells, attributing mainly to a dramatic reduction of the B220+ population. A considerable increase of Lin-/Sca1+/c-Kit+ (LSK) hematopoietic stem/progenitor cells was observed in the bone marrow of immunized mice, suggesting an induction of enhanced cell expansion by the vaccine adjuvant. Treatment of animals with L121-adjuvant also affects the differentiation of cells in the thymus with a decrease of the CD4+/CD8+ double-positive (DP) cells, and a concurrent increase of the CD4+ and CD8+ single positive (SP) populations. These experimental results suggested the differentiation effect of vaccine adjuvants on the hematopoietic system, accounting for the enhanced immunity after immunization.

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Huang, Bo, Yong Hua Duan, Sun Yong et Ming Jun Peng. « Elastic Properties and Electronic Structures of L1₂-TiAl₃ and L1₂-Ti(Al,Pt)₃ : A Density Functional Theory Investigation ». Materials Science Forum 817 (avril 2015) : 816–25. http://dx.doi.org/10.4028/www.scientific.net/msf.817.816.

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First-principles calculations have been carried out to investigate the elastic properties and electronic structures of L12-TiAl3and L12-Ti (Al, Pt)3. The optimized structural parameters were largely consistent with the experimental values. The electronic density of states (DOS) and the differences of charge density distribution were given. The independent single-crystal elastic constants and polycrystalline elastic parameters such as bulk modulusB, Young’s modulusE, shear modulusG, Poisson’s ratioνand anisotropy valueAhave been calculated by Voigt-Reuss-Hill averaging scheme. The results indicate that the L12-Ti (Al, Pt)3exhibits larger anisotropy and more ductile than L12-TiAl3.

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Nomura, Takaomi, Kohji Nakano, Yasushi Maki, Takao Naganuma, Takashi Nakashima, Isao Tanaka, Makoto Kimura, Akira Hachimori et Toshio Uchiumi. « In vitro reconstitution of the GTPase-associated centre of the archaebacterial ribosome : the functional features observed in a hybrid form with Escherichia coli 50S subunits ». Biochemical Journal 396, n^o 3 (29 mai 2006) : 565–71. http://dx.doi.org/10.1042/bj20060038.

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We cloned the genes encoding the ribosomal proteins Ph (Pyrococcus horikoshii)-P0, Ph-L12 and Ph-L11, which constitute the GTPase-associated centre of the archaebacterium Pyrococcus horikoshii. These proteins are homologues of the eukaryotic P0, P1/P2 and eL12 proteins, and correspond to Escherichia coli L10, L7/L12 and L11 proteins respectively. The proteins and the truncation mutants of Ph-P0 were overexpressed in E. coli cells and used for in vitro assembly on to the conserved domain around position 1070 of 23S rRNA (E. coli numbering). Ph-L12 tightly associated as a homodimer and bound to the C-terminal half of Ph-P0. The Ph-P0·Ph-L12 complex and Ph-L11 bound to the 1070 rRNA fragments from the three biological kingdoms in the same manner as the equivalent proteins of eukaryotic and eubacterial ribosomes. The Ph-P0·Ph-L12 complex and Ph-L11 could replace L10·L7/L12 and L11 respectively, on the E. coli 50S subunit in vitro. The resultant hybrid ribosome was accessible for eukaryotic, as well as archaebacterial elongation factors, but not for prokaryotic elongation factors. The GTPase and polyphenylalanine-synthetic activity that is dependent on eukaryotic elongation factors was comparable with that of the hybrid ribosomes carrying the eukaryotic ribosomal proteins. The results suggest that the archaebacterial proteins, including the Ph-L12 homodimer, are functionally accessible to eukaryotic translation factors.

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Denkova, Zapryana, Velichka Yanakieva, Bogdan Goranov, Teodora Tomova, Rositsa Denkova-Kostova, Nadia Oulahal, Georgi Kostov et Pascal Degraeve. « In vitro examination of the antimicrobial activity of a potentially probiotic Lactobacillus acidophilus strain against Escherichia coli, Staphylococcus aureus and Salmonella sp. » BIO Web of Conferences 45 (2022) : 02010. http://dx.doi.org/10.1051/bioconf/20224502010.

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An interesting property for many probiotic strains is to exhibit antimicrobial activity against pathogenic microorganisms. Therefore, the antimicrobial activity of the potentially probiotic strain Lactobacillus acidophilus L12 against Escherichia coli, Staphylococcus aureus and Salmonella sp. was examined in vitro through co-culturing of Lactobacillus acidophilus L12 with each of the pathogenic strains for 72 h. When cultured individually, all strains reached a final population always exceeding 1012 cfu/mL. When co-cultured with each of the pathogenic strains Lactobacillus acidophilus L12 population always exceeded 1012 cfu/mL, while the bacterial population of the pathogenic strains was reduced in a strain-specific manner. Lactobacillus acidophilus L12 demonstrated the highest inhibitory activity against the two Staphylococcus aureus strains, followed by the two E. coli strains, and the two Salmonella sp. strains tested. The kinetics of the co-culturing process of Lactobacillus acidophilus L12 with each of the test pathogenic strains was modelled using the logistic curve model. The obtained results show the strong antagonistic activity of the potentially probiotic strain Lactobacillus acidophilus L12, thus making it a promising candidate for inclusion in the composition of probiotic preparations and functional probiotic foods.

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Yamanaka, Kunika, Hisashi Sato et Yoshimi Watanabe. « Grain Refinement of Al Cast by Novel Al-Al_2.5Cu_0.5Ti Refiner Fabricated by Cold Pressing ». Materials Science Forum 783-786 (mai 2014) : 276–81. http://dx.doi.org/10.4028/www.scientific.net/msf.783-786.276.

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It is reported that alloying with Cu causes the transformation from the D022 type structure of Al3Ti into L12 cubic structure. Since the lattice constant of Al2.5Cu0.5Ti with L12 structure is a = 0.3927 nm, smaller disregistry value between Al and Al2.5Cu0.5Ti can be achieved. In this study, novel refiner for Al cast containing Al2.5Cu0.5Ti intermetallic compound particles with L12 structure is fabricated by cold pressing. It is found that the grains of Al cast are partially refined by using the novel refiner, although microstructure of Al grain is not homogeneous. In this way, the Al2.5Cu0.5Ti intermetallic compound particles with L12 structure can become favorable heterogeneous nucleation sites for Al cast.

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Yang, Ya-Wun, Yen-Chi Liu et Amit Gupta. « Roles of nitric oxide in hematopoietic differentiation induced by the vaccine adjuvant ». Journal of Immunology 200, n^o 1_Supplement (1 mai 2018) : 181.24. http://dx.doi.org/10.4049/jimmunol.200.supp.181.24.

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Abstract N(ω)-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide synthase (NOS), is involved in the pathogenesis of inflammatory disorders. The roles of nitric oxide (NO) in myeloid differentiation induced by the cancer vaccine adjuvant is unknown. The objective this study was to examine the effect of NOS inhibitor in the hematopoietic differentiation induced by the L121-adjuvant, an emulsion vaccine adjuvant containing Pluronic L121 previously shown to exhibit potent immune responses when combined with soluble protein antigens. C57BL/6J mice were vaccinated by subcutaneous injection on days 0 and 7 with 100 μl L121-adjuvant, containing 50 μg ovalbumin (OVA), followed by daily treatment with L-NAME (10 mg/kg). The animals were sacrificed on day 10, and bone marrow cells and splenocytes were isolated, lysed with the ammonium-chloride-potassium (ACK) lysing buffer, followed by flow cytometric analysis. Our results showed that vaccination of mice with the L121-adjuvant stimulated a considerable generation of CD11b+Gr-1+ myeloid populations in the bone marrow and spleens. Treatment of animals with L-NAME significantly upregulated the lineage−Sca-1+c-kit+ (LSK) frequency, but down-regulated the lineage− Sca-1− c-kit+CD16/CD32+ granulocytic/monocytic progenitor (GMP) populations in the bone marrow. These data demonstrated the involvement of NO in the modulation of granulocytic/monocytic differentiation and generation of inflammatory response in cancer vaccine adjuvant-mediate hematopoietic differentiation.

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Laughrea, Michael, et Eric Higgins. « ΔLys120, a mutation which destabilizes the ribosome-binding domain of ribosomal protein L7/L12 ». Biochemistry and Cell Biology 68, n^o 5 (1 mai 1990) : 832–38. http://dx.doi.org/10.1139/o90-123.

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Five-residue-long deletions centered on A1a63, A1a75, and Glu118 of ribosomal protein L7/L12 gave low mutant yields (5% or less) when the mutant genes were cloned in phage M13mp18 and controlled by the L10 promotor. Deletions of Glu118–Lys120 or Lys120 (the COOH-terminus of L7/L12) gave higher mutant yields, up to 50% with L7/L12ΔLys120. L7/L12ΔLys120 was not preferentially found in the S100 and not preferentially removed by LiCl washing, but was preferentially extracted from 70S ribosomes in the presence of 28–35% ethanol in 0.25–0.5 M NH4Cl. It follows that ΔLys120 destabilizes the ribosome-binding domain of ribosomal protein L7/L12 in an ethanol-containing solvent, which raises the question whether Lys120 is part of the ribosome-binding domain of L7/L12 during some step of protein synthesis or whether it is essential to preserve the conformation of the physiological ribosome-binding domain under structurally stressful conditions.Key words: ribosome, ribosomal protein L7/L12, site-directed mutagenesis.

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Cheng, Wenqiang, Guohao Qin, Muxin Yang, Wei Wang et Fuping Yuan. « Excellent tensile properties and deformation mechanisms in a FeCoNi-based medium entropy alloy with dual-heterogeneous structures ». Journal of Applied Physics 132, n^o 17 (7 novembre 2022) : 175111. http://dx.doi.org/10.1063/5.0118217.

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A dual-heterogeneous structure with both heterogeneous grain structure and coherent L12 nano-precipitate was obtained in a (FeCoNi)86Al7Ti7 medium-entropy alloy (MEA) after cold rolling, critical annealing, and aging treatments. The volume fraction of the L12 phase is found to increase from 0% to 34.5% after aging treatment, resulting in severer heterogeneity. The unaged samples of (FeCoNi)86Al7Ti7 MEA are found to have a much better synergy of strength and ductility as compared to the FeCoNi MEA. Moreover, the tensile properties for the aged samples of (FeCoNi)86Al7Ti7 MEA with dual-heterogeneous structures are even better than those for the corresponding unaged samples. The hetero-deformation-induced hardening plays a more important role in the aged samples than in the unaged samples, producing a higher density of geometrically necessary dislocations. High density dislocations are discovered in grain interiors for the aged samples and the deformation is dominated by the planar slip in the unaged samples. Meanwhile, a high density of dislocations are observed inside L12 nano-precipitates; thus, L12 nano-precipitates can be sheared by dislocations, indicating that L12 nano-precipitates are strong but deformable. The strong interactions between dislocations and L12 nano-precipitates should provide additional strain hardening for better tensile properties in the aged samples.

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Yang, Yunfei, Changhao Wang, Junhao Sun, Shilei Li, Wei Liu, Hao Wu et Jinshu Wang. « First-Principles Study of Mechanical and Thermodynamic Properties of Binary and Ternary CoX (X = W and Mo) Intermetallic Compounds ». Materials 14, n^o 6 (13 mars 2021) : 1404. http://dx.doi.org/10.3390/ma14061404.

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In this study, the structural, elastic, and thermodynamic properties of DO19 and L12 structured Co3X (X = W, Mo or both W and Mo) and μ structured Co7X6 were investigated using the density functional theory implemented in the pseudo-potential plane wave. The obtained lattice constants were observed to be in good agreement with the available experimental data. With respect to the calculated mechanical properties and Poisson’s ratio, the DO19-Co3X, L12-Co3X, and μ-Co7X6 compounds were noted to be mechanically stable and possessed an optimal ductile behavior; however, L12-Co3X exhibited higher strength and brittleness than DO19-Co3X. Moreover, the quasi-harmonic Debye–Grüneisen approach was confirmed to be valid in describing the temperature-dependent thermodynamic properties of the Co3X and Co7X6 compounds, including heat capacity, vibrational entropy, and Gibbs free energy. Based on the calculated Gibbs free energy of DO19-Co3X and L12-Co7X6, the phase transformation temperatures for DO19-Co3X to L12-Co7X6 were determined and obtained values were noted to match well with the experiment results.

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Harada, Yoshihisa, et David C. Dunand. « Microstructure and Hardness of Scandium Trialuminide with Ternary Rare-Earth Additions ». Materials Science Forum 539-543 (mars 2007) : 1565–70. http://dx.doi.org/10.4028/www.scientific.net/msf.539-543.1565.

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The microstructure of ternary Al3(Sc1-yREy) intermetallic compounds (where RE is one of the rare-earth elements La, Ce, Nd, Sm, Eu, Yb or Lu), was investigated as a function of RE concentration for 0<y≤0.75. Alloys with La, Ce, Nd, Sm or Eu additions consist of a L12 phase containing a dendritic second phase with D019 (La, Ce, Nd, Sm) or C11b (Eu) structure. Alloys with Yb or Lu additions show a single L12 phase. The RE solubility limits at 1373 K in the L12-Al3(Sc1-yREy) phase are very low for La, Nd, Ce and Eu (0.08-0.41 at.% or y=0.0032-0.0164), low for Sm (3.22 at.% or y=0.1288) and complete for Yb and Lu. The lattice parameter of the L12 solid-solution increases linearly with RE concentration and the magnitude of this effect is correlated with the atomic size mismatch between Sc and the RE elements. The Vickers micro-hardness of the L12 solid-solution increases linearly with increasing RE concentration.

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Xi, Shengkun, Jinxin Yu, Longke Bao, Liuping Chen, Zhou Li, Rongpei Shi, Cuiping Wang et Xingjun Liu. « Machine learning-accelerated first-principles predictions of the stability and mechanical properties of L12-strengthened cobalt-based superalloys ». Journal of Materials Informatics 2, n^o 3 (2022) : 15. http://dx.doi.org/10.20517/jmi.2022.22.

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As promising next-generation candidates for applications in aero-engines, L12-strengthened cobalt (Co)-based superalloys have attracted extensive attention. However, the L12 strengthening phase in first-generation Co-Al-W-based superalloys is metastable and both its solvus temperature and mechanical properties still need to be improved. Therefore, it is necessary to discover new L12-strengthened Co-based superalloy systems with a stable L12 phase by exploring the effect of alloying elements on its stability. Traditional first-principles calculations are capable of providing the crystal structure and mechanical properties of the L12 phase doped by transition metals but suffer from low efficiency and relatively high computational costs. The present study combines machine learning (ML) with first-principles calculations to accelerate crystal structure and mechanical property predictions, with the latter providing both the training and validation datasets. Three ML models are established and trained for predicting the occupancy of alloying elements in the supercell and the stability and the mechanical properties of the L12 phase. The ML predictions are evaluated using first-principles calculations and the accompanying data are used to further refine the ML models. Our ML-accelerated first-principles calculation approach offers more efficient predictions of the crystal structure and mechanical properties for Co-V-Ta- and Co-Al-V-based systems than the traditional counterpart. This approach is applicable to expediting crystal structure and mechanical property calculations and thus the design and discovery of other advanced materials beyond Co-based superalloys.

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Wang, Leini, Zhang Jian et Wei Ning. « Phonon, elastic and thermodynamic properties of L12 phase Rh3Zr under pressure from first-principles ». International Journal of Modern Physics C 28, n^o 07 (juillet 2017) : 1750098. http://dx.doi.org/10.1142/s012918311750098x.

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The phonon, elastic and thermodynamic properties of L12 phase Rh3Zr have been investigated by density functional theory approach combining with quasi-harmonic approximation model. The relaxed lattice parameters of L12 phase Rh3Zr at zero pressure are in good agreement with the experiment. To judge the stability of L12 phase Rh3Zr under high pressure, the phonon band structure has been studied. The results show that L12 phase Rh3Zr possesses dynamical stability in the pressure range from 0[Formula: see text]GPa to 80[Formula: see text]GPa due to the absence of imaginary frequencies. The pressure dependences of elastic constants [Formula: see text] have been analyzed. All the elastic constants of Rh3Zr in a wide pressure range (0–80[Formula: see text]GPa) meet general mechanical conditions, suggesting that L12 phase Rh3Zr is mechanically stable under pressure up to 80[Formula: see text]GPa. L12 phase Rh3Zr exhibits ductility under high pressure and the pressure can improve the ductility from the results of the value of [Formula: see text] and Poisson’s ratio [Formula: see text]. Hence, it is obvious that the mechanical properties of Rh3Zr can be improved under high pressure. Moreover, we have obtained the thermodynamic properties using the quasi-harmonic Debye model. We note that the effect of the temperature on the Debye temperature [Formula: see text] is smaller than that of the pressure. We believe that our result will be a good guidance to future works and applications.

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Gupta, Sonal, Surender Mohan, Vikas Kumar Somani, Somya Aggarwal et Rakesh Bhatnagar. « Simultaneous Immunization with Omp25 and L7/L12 Provides Protection against Brucellosis in Mice ». Pathogens 9, n^o 2 (24 février 2020) : 152. http://dx.doi.org/10.3390/pathogens9020152.

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Currently used Brucella vaccines, Brucella abortus strain 19 and RB51, comprises of live attenuated Brucella strains and prevent infection in animals. However, these vaccines pose potential risks to recipient animals such as attenuation reversal and virulence in susceptible hosts on administration. In this context, recombinant subunit vaccines emerge as a safe and competent alternative in combating the disease. In this study, we formulated a divalent recombinant vaccine consisting of Omp25 and L7/L12 of B. abortus and evaluated vaccine potential individually as well as in combination. Sera obtained from divalent vaccine (Omp25+L7/L12) immunized mice group exhibited enhanced IgG titers against both components and indicated specificity upon immunoblotting reiterating its authenticity. Further, the IgG1/IgG2a ratio obtained against each antigen predicted a predominant Th2 immune response in the Omp25+L7/L12 immunized mice group. Upon infection with virulent B. abortus 544, Omp25+L7/L12 infected mice exhibited superior Log10 protection compared to individual vaccines. Consequently, this study recommends that simultaneous immunization of Omp25 and L7/L12 as a divalent vaccine complements and triggers a Th2 mediated immune response in mice competent of providing protection against brucellosis.

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Zhao, Bing Bing, Xian Ping Dong, Feng Sun et Lan Ting Zhang. « Impact of L1₂-Ordered Precipitation on the Strength of Alumina-Forming Austenitic Heat-Resistant Steels ». Materials Science Forum 941 (décembre 2018) : 692–97. http://dx.doi.org/10.4028/www.scientific.net/msf.941.692.

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Alumina-forming austenitic (AFA) heat-resistance steels firstly developed by Yamamoto et al. at Oak Ridge National Laboratory have been reported as a new promising class of steels with potential for use in high temperature applications in recent years. The creep resistance of AFA steels is improved mainly by precipitation strengthening. Besides modifying the typical existing precipitates, i.e. MC and M23C6 type carbides, B2-NiAl and Fe2Nb-type Laves phase, introduction of coherent L12-ordered precipitate is highly desired. L12-ordered phase gamma prime (γ’) is the most important precipitate for high-temperature strengthening in Ni-based superalloys. In the present work, we demonstrate that addition of 2.8 wt. % Cu to an AFA steel promotes the formation of an L12-ordered phase with the dominating elements Ni, Cu and Al. TEM characterization after slow rate tensile tests indicated there were the different precipitation behaviours at 700°C and 750°C. It was revealed that the occurrence of L12-ordered Ni-Cu-Al phase depends on temperature and Ni content. This opens up new opportunities to promote the formation of L12-ordered phase in Fe-based austenitic heat-resistance steels and benefit high-temperature mechanical properties.

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Prameela, G. K. S., B. V. N. Phani Kumar, R. Ravikanth Reddy, A. Pan, J. Subramanian, Sugam Kumar, V. K. Aswal, Joachim Kohlbrecher, A. B. Mandal et S. P. Moulik. « Vesicle to micelle transition in the ternary mixture of L121/SDS/D2O : NMR, EPR and SANS studies ». Physical Chemistry Chemical Physics 19, n^o 47 (2017) : 31747–55. http://dx.doi.org/10.1039/c7cp06796h.

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Potez, L., A. Loiseau, S. Naka et G. Lapasset. « A study of Al2Ti precipitation in a Cu-modified Al3Ti alloy ». Journal of Materials Research 7, n^o 4 (avril 1992) : 876–82. http://dx.doi.org/10.1557/jmr.1992.0876.

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An equilibrium precipitation of Al2Ti is shown to occur within a Cu-modified Al2Ti alloy having the L12 structure. This precipitation is analyzed by conventional and high resolution electron microscopy and some insights are given concerning the mechanisms of nucleation and growth of Al2Ti within the L12 matrix. In the meantime, Vickers microhardness tests have been performed as a first approach to the mechanical behavior of this alloy and the results are compared to measurements obtained in an actual single-phased L12 compound. The Al2Ti precipitation seems to have an important hardening effect.

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Jang, Ok Jun, Cheol-Woong Yang et Dong Bok Lee. « Transmission Electron Microscopy Characterization of Thermomechanically Treated Al3Ti–(8, 10, 15)% Cr Intermetallics ». Microscopy and Microanalysis 19, S5 (août 2013) : 89–94. http://dx.doi.org/10.1017/s1431927613012403.

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AbstractThe ordered L12-type Al3Ti–(8, 10, 15)% Cr intermetallic compounds, namely, Al67Ti25Cr8, Al66Ti24Cr10, and Al59Ti26Cr15, were prepared by induction melting followed by thermomechanical treatment. Their microstructure, compositional variation, and crystal structure were characterized using X-ray diffraction, optical microscopy, and scanning and transmission electron microscopy equipped with energy-dispersive spectroscopy. The Al67Ti25Cr8 alloy consisted of the L12-Al3Ti matrix and precipitates of α2-Ti3Al, D022-Al3Ti, and γ-TiAl. The Al66Ti24Cr10 and Al59Ti26Cr15 alloys consisted of the L12-Al3Ti matrix and grains of α-TiAl and β-Cr.

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Puéchal, Xavier. « L12. Rheumatoid arthritis vasculitis ». La Presse Médicale 42, n^o 4 (avril 2013) : 527–30. http://dx.doi.org/10.1016/j.lpm.2013.01.031.

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Hagihara, Koji, Naoyuki Yokotani et Yukichi Umakoshi. « Temperature and Orientation Dependence of Fracture Behavior of Directionally Solidified Duplex-Phase Crystals Composed of Ni₃X-Type Intermetallic Compounds ». Materials Science Forum 512 (avril 2006) : 67–72. http://dx.doi.org/10.4028/www.scientific.net/msf.512.67.

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Fracture behaviors of three directionally solidified (DS) duplex-phase alloys composed of Ni3Nb(D0a)/Ni3Al(L12), Ni6TaAl(D024)/Ni3Al(L12) and Ni3Ti(D024)/Ni3Si(L12) phases, respectively were investigated by three-point bending tests, focusing on temperature and orientation dependence. The temperature-toughness relation showed dissimilar curves depending on alloy. The increasing rate of fracture toughness was the highest in the Ni3Al/Ni3Nb alloy with fine lamellar structure and was the lowest in the Ni3Al/Ni6TaAl alloy with rod-like precipitates. The controlling mechanism for the temperature dependence of fracture behavior of Ni3Al/Ni3Nb alloys was discussed.

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Chen, Xiangkai, Xiaohua Chen, Zidong Wang, Yanlin Wang, Kaixuan Chen et Yuzhi Zhu. « First Principles Investigation of the Effects of Chemical Short-Range Ordering Clusters on the Ideal Tensile Strength and Ductility of Aluminum Alloys ». Metals 12, n^o 12 (14 décembre 2022) : 2143. http://dx.doi.org/10.3390/met12122143.

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As important structural features of the metal materials, chemical short-range ordering clusters play a critical role in the mechanical properties of the materials. They have been discovered in dilute Al-alloy systems and are usually generated by annealing processes at high temperatures or by severe plastic deformation at room temperature. In the present work, systematic first-principle calculations were conducted to evaluate the influences of the chemical short-range ordering clusters L12-Al3Zr on the mechanical properties of the pure Al supercell. Results showed that the mechanical properties including both ideal tensile strength and ductility were improved simultaneously when the chemical short-range ordering clusters L12-Al3Zr were introduced to the pure Al. The larger the volume fraction of chemical short-range ordering clusters L12-Al3Zr, the larger the ideal tensile strength. The deformation charge density, the electron localization function and the density of state were computed to reveal the nature of the strengthening of the chemical short-range ordering clusters L12-Al3Zr on the pure Al supercell. It was found that excellent ideal tensile strength for the Al supercell with the chemical short-range ordering clusters L12-Al3Zr was due to strong charge accumulations and strong electronic interactions between the solute atoms Zr and the host Al atoms. In addition, the Pugh ratio (B/G) and ratio (Wsep/Gdisl) of the work of the separation Wsep to the work of dislocation emission Gdisl were computed to reveal the effect of the chemical short-range ordering clusters L12-Al3Zr on the ductility of the Al supercell. Results showed that the addition of L12-Al3Zr chemical short-range ordering clusters addition to pure Al supercell brought about an increase in ductility as compared to pure Al supercell, which is ascribed to large the Pugh ratio B/G and ratio (Wsep/Gdisl) of the work of the separation Wsep to the work of dislocation emission Gdisl. This work is important for simultaneously improving the tensile strength and ductility of Al alloys.

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Kang, Chang Won, Hee Sup Jang et Seon Jin Kim. « Thermal Stability and Mechanical Properties of Nanocrystalline L1₂ Al₃Hf and (Al+12.5 at.%Zn)₃Hf Prepared by MA and SPS ». Materials Science Forum 449-452 (mars 2004) : 809–12. http://dx.doi.org/10.4028/www.scientific.net/msf.449-452.809.

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Thermal stability and mechanical properties of L12 Al3Hf and (Al+12.5 at.%Zn))3Hf synthesized by mechanical alloying(MA) and spark plasma sintering(SPS) were investigated. Nanocrystalline L12 phase was produced after MA for 8 and 10 hrs in Al3Hf and (Al+12.5 at.%Zn))3Hf powders, respectively. The grain sizes were reduced to about 10 nm in both systems after MA for 20 hrs. After SPS, L12 phase was maintained only in Zn added system. In (Al+12.5 at.%Zn))3Hf, L12 to D023 phase transformation was started at about 850°C and finished at about 1150°C Microhardness was decreased with increasing the annealing temperature while fracture toughness was increased due to the grain growth. Fracture toughness of (Al+12.5 at.%Zn))3Hf was greater than that of Al3Hf in all annealing temperatures. Fracture toughness of (Al+12.5 at.%Zn))3Hf after annealing at 1200°C was about 5.38 MPam1/2.

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Iwasaki, N., K. Xiang, M. Seino et G. I. Bell. « Dinucleotide repeat polymorphism in D20S17 (CRI-L127) ». Nucleic Acids Research 19, n^o 24 (1991) : 6970. http://dx.doi.org/10.1093/nar/19.24.6970-a.

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Bendersky, L. A., F. S. Biancaniello et M. E. Williams. « Evolution of the two-phase microstructure L12 + DO22 in near-eutectoid Ni3(Al,V) alloy ». Journal of Materials Research 9, n^o 12 (décembre 1994) : 3068–82. http://dx.doi.org/10.1557/jmr.1994.3068.

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Transmission electron microscopy and powder x-ray diffraction methods have been used to investigate the evolution of two-phase (L12 + DO22) microstructures from the quenched fcc phase of the Ni-5Al-20V (at. %) alloy. The microstructure after annealing in a temperature range from 650 to 900 °C differs from the eutectoid structure which might be expected for the alloy according to the eutectoid-type phase diagram of the Ni3Al-Ni3V section. This structure results from fast kinetics of ordering in the fcc → L12 and fcc → DO22 phase transitions. Four main stages in the microstructural evolution were observed. Stage I is the formation of spheroidal coherent L12 clusters in a disordered fcc matrix. During stage II the L12 clusters transform into cuboidal precipitates, and the fcc matrix orders into three DO22 variants (which may have interfaces that are wetted by thin fcc layers). In stage III accommodation of misfit (elastic energy) between different phases and variants occurs by formation of (110) twins or a single variant of the DO22 phase and tetragonally strained lamellae of the L12 phase. Stage IV is a discontinuous coarsening process in which a coarse incoherent two-phase structure replaces the fine coherent one. Grains of the coarse structure are nucleated on high-angle boundaries of primary fcc or other surfaces. Many of the grains are found twinned.

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Luo, Deyan, Bing Ni, Peng Li, Wei Shi, Songle Zhang, Yue Han, Liwei Mao, Yangdong He, Yuzhang Wu et Xiliang Wang. « Protective Immunity Elicited by a Divalent DNA Vaccine Encoding Both the L7/L12 and Omp16 Genes of Brucella abortus in BALB/c Mice ». Infection and Immunity 74, n^o 5 (mai 2006) : 2734–41. http://dx.doi.org/10.1128/iai.74.5.2734-2741.2006.

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ABSTRACT This study was designed to evaluate the immunogenicity and the protective efficacy of a divalent fusion DNA vaccine encoding both the Brucella abortus L7/L12 protein (ribosomal protein) and Omp16 protein (outer membrane lipoprotein), designated pcDNA3.1-L7/L12-Omp16. Intramuscular injection of this divalent DNA vaccine into BALB/c mice elicited markedly both humoral and cellular immune responses. The specific antibodies exhibited a dominance of immunoglobulin G2a (IgG2a) over IgG1. In addition, the dual-gene DNA vaccine elicited a strong T-cell proliferative response and induced a large amount of gamma interferon-producing T cells upon restimulation in vitro with recombinant fusion protein L7/L12-Omp16, suggesting the induction of a typical T-helper-1-dominated immune response in vivo. This divalent DNA vaccine could also induce a significant level of protection against challenge with the virulent strain B. abortus 544 in BALB/c mice. Furthermore, the protection level induced by the divalent DNA vaccine was significantly higher than that induced by the univalent DNA vaccines pcDNA3.1-L7/L12 or pcDNA3.1-Omp16. Taken together, the results of this study verify for the first time that the Omp16 gene can be a candidate target for a DNA vaccine against brucellosis. Additionally, a divalent genetic vaccine based on the L7/L12 and Omp16 genes can elicit a stronger cellular immune response and better immunoprotection than the relevant univalent vaccines can.

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De Hosson, J. T. M. « Superlattice dislocations in L12 ordered alloys and in alloys containing L12 ordered precipitates ». Materials Science and Engineering 81 (août 1986) : 515–23. http://dx.doi.org/10.1016/0025-5416(86)90288-0.

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