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1
Ozato, Kenjiro. « "The Biology of the Medaka" (in Japanese) by Takashi Iwamatsu(Book Review) ». Laboratory of Freshwater Fish Stocks BioScience Center Nagoya University, 1993. http://hdl.handle.net/2237/13786.
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Andrews, Adam Lee. « The role of female preference in sexual dimorphism of Japanese medaka (Oryzias latipes) ». Connect to resource, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1172768559.
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Oxendine, Sharon L. Padilla Stephanie. « Exploring a fish model of developmental neurotoxicity using the Japanese medaka fish (Oryzias latipes) ». Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2006. http://dc.lib.unc.edu/u?/etd,676.
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Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2006.Title from electronic title page (viewed Oct. 10, 2007). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Curriculum of Toxicology." Discipline: Toxicology; Department/School: Medicine.
4
Sakaizumi, Mitsuru, Yasuyuki Kurihara et Yasuko Hyodo-Taguchi. « Random genomic clones as a tool to construct genetic map in Japanese medaka, Orizias latipes ». Laboratory of Freshwater Fish Stocks, Nagoya University, 1992. http://hdl.handle.net/2237/13768.
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Liebl, Andrea. « Chemical, Toxicological, and Microbial Characterization of New Orleans Sediments Following Hurricane Katrina ». ScholarWorks@UNO, 2007. http://scholarworks.uno.edu/td/592.
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On August 29, 2005 Hurricane Katrina struck the Gulf Coast and storm surges breached levees flooding much of New Orleans, Louisiana. One month after the storm, sediment was collected and toxicity was tested using Japanese medaka (Oryzias latipes) embryos. Sediments with the highest contaminant levels showed the highest embryonic mortality and most delayed development. However, no sediment caused an increased mutant frequency. When the most contaminated site was resampled in February, 2006 contaminant levels and toxicity decreased. During toxicity testing, approximately 20% of embryos incubated with sediment from one of these sites died and turned red. A red bacterium was isolated that is Gram-negative, cocco-baccilus, non-motile, and most similar to Hahella chejuensis based on genetic and metabolic tests. This bacterium caused 100% infection at 108 bacterial cells per ml and variable infection at lower doses. This study was the first to examine biological effects of exposure to post-Hurricane Katrina sediments.
6
Tulou, Kimberly E. « Vitellogenin induction in male Japanese medaka exposed to the chemotherapeutics : oxytetracycline, Romet-30, and copper sulfate ». Thesis, Virginia Tech, 2006. http://hdl.handle.net/10919/41438.
Texte intégralRésumé :
The specificity of a medaka vitellogenin (Vtg) enzyme-linked immunosorbent assay (ELISA) kit was tested on adult hybrid tilapia to determine if vitellogenin could be successfully measured in tilapia using this method. Adult hybrid (Orechromis niloticus x Oreochromis mossambicus x Oreochromis aureus) tilapia were injected with 17â-estradiol (E2) at 5 ìg/g body weight (b.w.) in two separate experiments and exposed to 2.5 ìg/g b.w. E2 through aquaria water in a third experiment. All fish were exposed to solvent controls (50/50 PBS/ethanol, or 59/41 PBS/ ethanol) proportional to the volume of estradiol solvent used. Experiment 1 was terminated due to a decrease in fish health and unexpected mortalities. Experiment 2 was continued though there was again a decrease in fish health and mortalities before experiment was completed. According to one-way ANOVA, Experiment 2 treatment groups were significantly different (P<0.05); however, Tukeyâ s pairwise comparisons showed no differences between treatment groups. Experiment 3 results showed statistical differences between some treatment groups (P<0.001). However, maximum vitellogenin levels reached 1250 ng/mL Vtg, which were much lower than induced vitellogenin levels from literature (0.002 mg/mL to 6 mg/mL). Typical induction trends should be as follows: male control
7
Gray, Michelle Anya. « Assessing the effects of xenoestrogens on the reproductive potential of fish using Japanese medaka, Oryzias latipes ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ40472.pdf.
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Davis, Seth R. « Reproductive consequences of exposure to sediment extracts from the South Branch of the Potomac River on Japanese medaka (Oryzias latipes) ». Morgantown, W. Va. : [West Virginia University Libraries], 2007. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=5521.
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Thesis (M.S.)--West Virginia University, 2007.Title from document title page. Document formatted into pages; contains viii, 69 p. : ill., maps. Includes abstract. Includes bibliographical references.
9
Paulos, Peter M. « Reproductive and Growth Responses of the Fathead Minnow (Pimephales Promelas) and Japanese Medaka (Oryzias Latipes) to the Synthetic Progestin, Norethindrone ». Thesis, University of North Texas, 2011. https://digital.library.unt.edu/ark:/67531/metadc68029/.
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A commonly prescribed contraceptive, the synthetic progestin norethindrone (NET) inhibits ovulation in humans. However, ecotoxicological data are lacking. Preliminary tests produced an LC50 for NET of > 1.0 mg/L (96-hour, fathead minnow (FHM) and medaka) and a NOEC of 242.0 µg/L, a LOEC of 485.0 µg/L (7-day, growth for FHM and medaka). Reproductive testing revealed a LOEC for fecundity of 24.1 ng/L (21 days, medaka). Further testing confirmed the LOEC of 24.1 ng/L while defining a NOEC of 4.7 ng/L (28 days, medaka). Effect of NET in medaka life-cycle exposure at concentrations exceeding 4.7 ng/L was evident. Few females were present in the 24.7 ng/L exposure concentration, with none in the 104.6 ng/L. Egg production was significantly reduced at concentrations exceeding 4.7 ng/L. Additionally, weight, condition factor and somatic indices were significantly different in males exposed to concentrations exceeding 4.7 ng/L. For fecundity and sexual differentiation; the NOEC was 4.7 ng/L, the LOEC 24.6 ng/L; growth and somatic indices, the NOEC was more appropriately 0.9 ng/L, with effect evident at 4.7 ng/L. Sexual differentiation of the F1 population was similar to the F0. A defining result of this test was development of exceptionally large ovaries in NET- exposed female medaka, perhaps indicative of a threshold limit for exposure in these fish. Results of FHM life-cycle testing were similar, establishing a NOEC for fecundity of 0.9 ng/L, a LOEC of 4.8 ng/L. NET's inhibitory effect on gonadal development was obvious; GSI NOEC for males, 4.8 ng/L, and histological examination confirmed the presence of intersex development at elevated concentrations. Normal physical development and growth were impaired, generally at concentrations exceeding 24.1 ng/L. At exposure concentrations exceeding 4.8 ng/L, external sexual confirmation of fish was difficult; LOEC for finspot development in females, 4.8 ng/L. Sexual determination of the 97.1 ng/L exposure group was impossible; externally, all fish appeared male and internal examination revealed no gonadal development.
10
Robb, Stacey E. « Aggression, monopolization and growth depensation within groups of Japanese medaka, Oryzias latipes, interactions between the temporal and spatial clumping of food ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq25980.pdf.
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Zhang, Xiaowei. « Application of toxicogenomic approaches to study chemical-induced effects on the hypothalamic-pituitary-gonadal (HPG) axis of the Japanese medaka (Oryzias latipes) ». Diss., Connect to online resource - MSU authorized users, 2008.
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Thesis (Ph. D.)--Michigan State University. Dept. of Zoology & Environmental Toxicology, 2008.Title from PDF t.p. (viewed on Mar. 30, 2009) Includes bibliographical references. Also issued in print.
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Fukutani, Naoto. « Association of Varus Thrust With Pain and Stiffness and Activities of Daily Living in Patients With Medial Knee Osteoarthritis ». 京都大学 (Kyoto University), 2016. http://hdl.handle.net/2433/215467.
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© [2015] American Physical Therapy Association.Kyoto University (京都大学)
0048
新制・課程博士
博士(人間健康科学)
甲第19641号
人健博第33号
新制||人健||3(附属図書館)
32677
京都大学大学院医学研究科人間健康科学系専攻
(主査)教授 市橋 則明, 教授 山田 重人, 教授 妻木 範行
学位規則第4条第1項該当
13
Barjhoux, Iris. « Étude de la biodisponibilité et de la toxicité de polluants chimiques à risque dans les sédiments aquatiques vis-à-vis des premiers stades de développement d’un poisson modèle, Oryzias latipes ». Thesis, Bordeaux 1, 2011. http://www.theses.fr/2011BOR14442/document.
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Les sédiments agissent comme de véritable puits pour certains polluants organiques persistants et métaux représentant ainsi une source de contamination secondaire pour les milieux aquatiques. Dans ce contexte, un test embryo-larvaire utilisant le Medaka japonais (Oryzias latipes) a été développé afin d’évaluer la toxicité des polluants fixés aux particules sédimentaires. Le bioessai consiste à maintenir les embryons de Medaka en contact direct avec le sédiment durant tout leur développement embryonnaire puis à évaluer les effets létaux et sublétaux induits au niveau phénotypique et moléculaire chez les embryons et larves exposés. Tout d’abord, le bioessai a été appliqué à la caractérisation de la toxicité et des modes d’action de substances organiques et métalliques pures. Le pyrène et son dérivé, le méthylpyrène, se sont avérés fortement tératogènes avec un spectre de malformations rappelant le syndrome de la maladie du sac bleu. L’étude du profil d’expression génique a révélé la perturbation de certains processus cellulaires notamment la voie AhR, le métabolisme mitochondrial et la voie de signalétique et de métabolisation des rétinoïdes. Le cuivre et le cadmium ont induit de nombreuses malformations squelettiques et cardio-vasculaires, des dommages à l’ADN ainsi que l’altération de l’expression de gènes impliqués dans le métabolisme mitochondrial, la régulation du cycle cellulaire et la réparation de l’ADN. Enfin, le bioessai a été appliqué à l’évaluation du potentiel toxique de sédiments naturels du système Lot-Garonne et du Bassin d’Arcachon. L’ensemble des sédiments testés a montré un large spectre d’effets tératogènes et seuls quelques-uns ont conduit à des effets létaux. Par ailleurs, l’expression plusieurs gènes impliqués dans le métabolisme mitochondrial, la voie AhR, le contrôle du cycle cellulaire, la réparation de l’ADN et la détoxication des métaux a été modifiée lors de l’exposition à certains sédiments contaminés de la zone d’étudeAquatic sediments act as real sink for numerous anthropogenic chemicals such as persistent organic pollutants and heavy metals as well as a secondary contamination source. In this context, a fish embryo-larval assay was developed using the Japanese Medaka (Oryzias latipes) as a test organism to evaluate de toxicity of particle-bound contaminants. In this aim, fish embryos are kept in direct contact to sediment during their whole embryonic development and induced lethal and sub-lethal effects are measured at the phenotypical and molecular levels. This test was first applied to the toxicity evaluation and modes of action characterization of organic and metallic single compounds. Pyrene and its alkylated derivative, methylpyrene, revealed a high teratogenic potency inducing developmental deformities similar to the blue sac disease syndrome. Gene expressions response pattern using RT-PCR method highlighted the alteration of several cellular process including AhR pathway, mitochondrial metabolism and retinoids signaling and metabolism pathways. Copper and cadmium mainly induced numerous skeletal and cardiovascular injuries, DNA damage as well as modifications of gene expression involved in mitochondrial metabolism, DNA damage repair and cell cycle regulation. Finally, the Medaka embryo-larval assay was applied to analyze the toxic potential of natural sediments form the Lot-Garonne system and the Arcachon bay. All tested sediments showed a wide range of teratogenic effects but only few of them revealed to be acutely toxic. Moreover, the expression of several genes involved in mitochondrial metabolism, AhR pathway, cell cycle arrest, DNA repair and heavy metals detoxication proved to be modified in the presence of some contaminated sediments from the studied area
14
Leaf, Robert Thomas. « The Evolutionary Effects of Fishing : Implications for Stock Management and Rebuilding ». Diss., Virginia Tech, 2010. http://hdl.handle.net/10919/28636.
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Recent empirical studies have demonstrated inter-generational morphological and life-history
changes in fish stocks that have been impacted by size-selective harvest. Evolutionary processes
in biological populations occur through differential survival and reproductive success based, in
part, upon individual phenotypic variability. Fishing is a source of directional selection resulting
in the directed removal of some phenotypes; however, many aspects of the evolutionary effects
of fishing remain have yet to be described. In order to better understand the life-history and
morphological changes that occur as a result of size-selective fishing, and their effect on fishery
dynamics, I first determined the suitability of Japanese medaka (Oryzias latipes) for selection
experiments. I performed selection experiments using Japanese medaka and report how
morphology and life-history characteristics changed over multiple generations of selection. I
then used these patterns of change in life-history and morphology to validate individual-based
simulation candidate models to test general mechanisms of life-history relationships. Finally, I
applied the individual-based simulation modeling approach in order to describe how biological
and fishery characteristics change in a large, age-structured population exposed to size-selective
fishing over multiple generations. I found that the Japanese medaka has attractive characteristics
for biological investigation. The selection experiments indicated large changes in the age-atmaturity,
including a nearly 50% decrease over four generations in the most intense sizeselective
removal regimes. However, I did not observe significant changes in length-at-age or
weight-at-age over the course of the experiment. Candidate simulation models were poor at
predicting some aspects of the life-history characteristics of Japanese medaka. The simulation
model to determine fishery characteristics predicted large decreases in yield and egg production
as a result of decreases in length-at-age. Understanding the relationships of life-history
characteristics and their role in determining population resilience is a step toward understanding
the importance of evolutionary processes in fishery management.Ph. D.
15
Chong, Samuel Siong Chuan. « Evaluation of a fish gene transfer system : expression, fate, and germline transmission of CAT recombinant plasmid and phage sequences microinjected into newly fertilized eggs of the Japanese medaka, Oryzias latipes (Temminck & ; Schlegel) ». Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/27407.
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The creation of 'transgenic' animals has provided insights into mechanisms of gene regulation, as well as opened up a new avenue for genetic improvement of livestock, including fish.
In this thesis, the suitability of the Japanese ricefield fish or 'medaka' (Oryzias latipes) as a gene expression system was evaluated. The procaryotic chloramphenicol acetyltransferase (CAT) gene regulated by a double eucaryotic promoter-enhancer region was chosen as a reporter. This reporter was introduced as either a supercoiled or linear recombinant plasmid (pUSVCAT), as a phage, or as purified phage DNA. DNA or phage was microinjected into the cytoplasm of newly fertilized medaka eggs at the 1-2 cell stage. Expression and fate of the injected DNA or phage were monitored by harvesting medaka at various developmental stages and performing CAT enzyme assays and Southern blot analyses, respectively. Several injected eggs were allowed to develop to sexual maturity, and their offspring were pooled and tested by CAT enzyme assay for inheritance of the CAT sequences.
The patterns of expression of injected supercoiled and linear pUSVCAT DNA were very similar, indicating that DNA conformation does not affect the efficiency of expression. CAT enzyme activity was detectible from the early high blastula stage (4 hr post-injection), was strongest at the late gastrula/early neurula stage (1 day post-injection), and was sustained but slightly weaker in the one-week old embryo. Expression was significantly reduced in hatchlings (2 weeks post-injection), varying noticeably among the individuals analysed. CAT expression was still detectible in free-swimming fish (4 weeks post-injection). Recombinant CAT phage particles or purified CAT phage DNA were also able to express the CAT gene up to the free-swimming fish stage. However, in these treatments, the strongest CAT expression was seen in the one-week old embryo instead of in the gastrula/neurula, raising the possibility of a role played by different vector sequences on gene expression.
Studies on the fate of injected supercoiled and linear pUSVCAT revealed conversion of the input forms to high molecular weight head-to-tail and randomly oriented concatemers respectively. Total plasmid DNA increased rapidly during cleavage and gastrulation, indicative of plasmid replication, whereas degradation of plasmid sequences was observed by the early high blastula stage. In the gastrula/neurula derived from injection of supercoiled pUSVCAT, total plasmid DNA increased ten-fold, whereas injection of linear pUSVCAT resulted in a 12-fold increase at the same stage. In both cases, most of the observed increase was contributed by the high molecular weight concatemers. The amount of plasmid DNA decreased after the gastrula/neurula stage, and this DNA was exclusively of the high molecular weight form at hatching and could persist to the free-swimming stage.
Neither the DNA from injected CAT phage particles nor the injected purified CAT phage DNA appeared to be concatenated during early embryogenesis. In both cases, however, the phage DNA appeared as higher molecular weight DNA by the one-week old embryonic stage, probably formed by covalent end-to-end ligations. DNA of CAT phage particles did not increase until after the early high blastula stage, but by the flat blastula stage (10 hr post-injection) a three-fold increase over the input amount was observed. There was no significant increase at the gastrula/neurula stage, nor was there an immediate decrease thereafter. Injected purified CAT phage DNA increased through the stages of cleavage and gastrulation, the gastrula/neurula having seven-fold more CAT phage DNA than that injected, and decreased thereafter. Both DNA of injected phage particles and injected phage DNA could persist to the free-swimming stage. CAT gene expression was detected in a number of pooled offspring from several DNA and phage-treated fish, indicating inheritance of the input sequences. The data in this study suggest that the germline-positive parents are probably mosaic for the presence of the CAT sequences, and that germline transmission is possible with plasmid DNA of both conformations, DNA-carrying phage particles, or purified phage DNA.
The above results, coupled with the ease of handling and manipulation of the medaka embryo, strongly favour the use of the medaka as a transient expression and transgenic animal model.Science, Faculty of
Zoology, Department of
Graduate
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Pannetier, Pauline. « Outils alternatifs à l'expérimentation animale pour l'évaluation de la toxicité des contaminants de l'environnement : lignées cellulaires et tests embryo-larvaires chez un poisson modèle le Médaka japonais, Oryzias latipes ». Thesis, Bordeaux, 2018. http://www.theses.fr/2018BORD0050/document.
Texte intégralRésumé :
Les poissons sont parmi les modèles aquatiques les plus utilisés pour l’évaluationdes dangers des substances chimiques avant leur mise sur le marché européen(REACh (Registration, Evaluation, Autorisation and Restriction of CHemicals)).Actuellement, cette réglementation ainsi que la directive européenne sur la protectiondes animaux utilisés à des fins scientifiques (2010/63/UE) promeuvent la mise enplace de méthodes alternatives en expérimentation animale. Ces méthodes ont pourbut de remplacer les modèles animaux chaque fois que cela est possible, de réduirele nombre d’animaux utilisés en expérimentation et d’optimiser la méthodologieappliquée aux animaux. Cette thèse vise à comparer la sensibilité de deux de cesoutils alternatifs, les lignées cellulaires et les tests embryo-larvaires poissons, enutilisant des toxiques modèles puis mettre en oeuvre ces outils pour évaluer la toxicitéde mélanges environnementaux de polluants : microplastiques et hydrocarbures(pétrole Arabian Light). Les composés modèles (B(a)P, Cd, MMS, PCB126) ont permisde valider trois biomarqueurs : la production d’espèces réactives de l’oxygène surlignées cellulaires de poissons, le test micronoyaux par cytométrie en flux et lechallenge infectieux sur larves de Médaka. Ces biomarqueurs associés à des analyseschimiques, biochimiques et physiologiques ont permis d’évaluer la toxicité demélanges environnementaux d’hydrocarbures (extrait aqueux de pétrole Arabian =WAF) et de microplastiques. Les WAF présentent une toxicité sur les lignéescellulaires de poissons (induction de l’activité EROD, génotoxicité) ainsi que surembryons et larves de Médaka (comportement, biométrie, EROD, génotoxicité). Lespoissons comme les cellules sont particulièrement sensibles au WAF de l’ArabianLight. La contamination aux microplastiques (MP) environnementaux a révélé unetoxicité des contaminants associés au MP sur les lignées cellulaires et les larves depoisson. L’ingestion de particules de plastiques par les larves a induit des effets létauxet sublétaux (biométrie, comportement et EROD). L’ingestion des particules plastiquespar les larves a été confirmée par microscopie biphotonique. Cette étude a permisd’évaluer la sensibilité et la complémentarité de ces outils in vitro pour l’évaluation desdangers et des risques associés aux substances chimiquesFish are among the most widely used aquatic model for assessing the chemicalthreat before they are placed on the European market (REACh (Registration,Evaluation, Authorization and Restriction of CHemicals)). Currently, this regulation aswell as the European directive on the protection of animals used for scientific purposes(2010/63/EU) promote the establishment of alternative methods in animal testing.These methods are intended to replace animal models whenever possible, to reducethe number of animals used in experiments and to optimize the methodology appliedto animals. This thesis aims to compare the sensitivity of two of these alternative tools,cell lines and fish embryo-larval tests, using model toxicants. Then, these tools wereused to evaluate the toxicity of environmental mixtures of pollutants: microplastics andhydrocarbons (Arabian Light oil). The model compounds (B(a)P, Cd, MMS, PCB126)valid three biomarkers: reactive oxygen species (ROS) production on fish cell lines,micronucleus assay by flow cytometry and infectious challenge on Medaka larvae andjuveniles. These biomarkers combined with chemical, biochemical and physiologicalanalyzes have assessed toxicity of environmental mixtures of hydrocarbons (aqueousextract of Arabian oil = WAF) and microplastics. WAF are toxic to fish cell lines (ERODactivity induction and genotoxicity) as well as to Medaka embryos and larvae (behavior,biometrics, EROD, genotoxicity). Fish like cells seem to be particularly susceptible toArabian Light WAF. Contamination with environmental microplastics (MP) hasrevealed a toxicity of MP-associated contaminants on cell lines and fish larvae. Theingestion of plastic particles by the larvae induced lethal and sublethal effects(biometrics, behavior and EROD). The ingestion of the plastic particles by the larvaewas confirmed by two-photon microscopy. This study assessed the sensitivity andcomplementarity of these in vitro tools for assessing hazards and risks associated withchemicals
17
Le, Bihanic Florane. « Effets des hydrocarbures aromatiques polycycliques sur les stades précoces de poissons modèles : développement de bioessais et étude comparée de mélanges ». Phd thesis, Université Sciences et Technologies - Bordeaux I, 2013. http://tel.archives-ouvertes.fr/tel-01068302.
Texte intégralRésumé :
La majorité des polluants organiques persistants, tels que les hydrocarbures aromatiques polycycliques (HAP), sont hydrophobes et de ce fait s'accumulent au cours du temps dans les sédiments aquatiques. Dans ce contexte, des tests ont été mis en œuvre sur embryons et pro-larves de truite arc-en-ciel Oncorhynchus mykiss et de médaka japonais Oryzias latipes pour étudier la toxicité de ces contaminants. Un sédiment artificiel et de nouveaux critères d'effets sur l'intégrité de l'ADN et l'activité natatoire ont été développés pour améliorer la reproductibilité et la sensibilité des tests. Ces bioessais ont été appliqués à la comparaison de la toxicité de trois mélanges de HAP : un extrait pyrolytique et deux extraits issus d'un pétrole léger Arabian Light et lourd de type Erika. Les trois mélanges de HAP ont été enrobés sur un sédiment artificiel ou sur des graviers à des concentrations comparables à celles retrouvées dans l'environnement. L'exposition des embryons à ces matrices contaminées a provoqué des perturbations de l'expression de gènes impliqués dans le fonctionnement mitochondrial, le métabolisme de la vitamine A, la formation de dommages à l'ADN ainsi que la synthèse d'hormones. Parallèlement ces mélanges ont induit des effets tératogènes, des perturbations de l'activité natatoire et des effets génotoxiques. Le degré de toxicité et le spectre d'effets induits étaient différents selon la composition des mélanges testés. Les mélanges pétrogéniques présentant une forte proportion en HAP de faibles poids moléculaires et HAP alkylés, se sont avérés plus toxiques envers les jeunes stades de développement de poissons que le mélange pyrolytique, composé en majorité de HAP à hauts poids moléculaires non substitués. Des résultats comparables ont été obtenus avec les modèles médaka japonais et truite arc-en-ciel.
18
Cho, Tzu-Chun, et 卓姿均. « In Japanese colonial period, Girl’s Meddle Schools and Taiwan Women ». Thesis, 2015. http://ndltd.ncl.edu.tw/handle/31785997843636913078.
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碩士國立臺灣師範大學
臺灣史研究所
103
In Japanese colonial period, Girl’s Meddle Schools is four years of secondary education for girls established. Its educational aims: cultivate good wife and wise mother. In Taiwan, girl after graduating primary education, secondary schools may further studies, but after they lack higher education institutions, and need to go abroad to further education. So, research on Girls' Meddle School set, useful understanding of national education, female education during the Japanese colonial period. Minority-secondary education by women, demonstrated excellent achievement. Educated women have more choices of life. In this paper, to discuss changes in the spirit of the Taiwan Women to become Girls' Meddle School graduate in Japanese colonial period. With「Girl’s Meddle Schools and Local Society」、「Girl’s Meddle School Educational content」、「Girl’s Meddle Schools and Taiwan Women」thinking about the meaning of education for women
19
Chang, Keng-Hao, et 張耿豪. « A Study on Intercalation and Administration of Taiwanese Collaborator Medal Rules in Japanese Ruling Period ». Thesis, 2008. http://ndltd.ncl.edu.tw/handle/83505827061116921818.
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Chen, Guan-Lin, et 陳冠霖. « Acid-base regulation and stress-resistant of Japanese medaka embryos under ocean acidification ». Thesis, 2014. http://ndltd.ncl.edu.tw/handle/02926700294638778271.
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Kennedy, Sharilyn. « Embryotoxicity of dioxin-like chemicals extracted from American eels (Anguilla rostrata) from the St. Lawrence River System ». Thesis, 2010. http://hdl.handle.net/1974/6008.
Texte intégralRésumé :
The American eel (Anguilla rostrata) has suffered a serious population decline in Lake Ontario since the early 1980s due to a decline in recruitment of juveniles migrating from the Sargasso Sea. This has resulted in the closure of the Lake Ontario fishery in 2004 and its listing as endangered under the Ontario Species at Risk act in June of 2008 in Ontario. Due to their complex life cycle, little is known about eels once they leave their freshwater habitats and migrate to the Sargasso Sea to reproduce. Ocean conditions, habitat destruction, disease, reduced lipid content, over-fishing, physical barriers (hydroelectric dams), and chemical contamination are all possible reasons for recruitment decline and may be acting cumulatively. Maternally derived dioxin-like contaminants (DLCs) accumulated during the growth phase of eels in Lake Ontario are toxic to fish embryos, and embryotoxicity is expressed as a series of malformations known as blue sac disease (BSD). I assessed whether these toxicants are in high enough concentrations in sexually maturing, eels to be embryotoxic to their offspring, as assessed by using Japanese medaka (Oryzias latipes), a surrogate species. Medaka embryos were first injected with 2,3,7,8-tetracholordibenzo-p-dioxin (TCDD) to establish their sensitivity to this test chemical expressed as an 11-day EC50 of 3.79 pg/mg, for the induction of BSD. Medaka embryos were injected with eel extracts and their response compared to the TCDD toxicity curve to assess whether extracts caused developmental problems and to estimate the relative concentration of DLCs. Eel extracts from all collection sites caused no dioxin-like toxicity to Japanese medaka embryos. However, significantly higher toxicity at 10 eeq relative to triolein was found for all extracts with no differences among sites, suggesting the presence of non-dioxin-like toxicants. The low level of maternal tissue contamination by DLCs implied by this bioassay is mirrored in chemical monitoring of persistent organic pollutants in Lake Ontario fish. If correct, the low levels of toxicity of extracts to embryos could contribute to the observed increase of eels entering L. Ontario from 2003 to 2008.Thesis (Master, Biology) -- Queen's University, 2010-09-01 09:31:19.466
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Lin, Chia-Cheng, et 林家正. « Molecular Physiological Study on Na+ uptake/acid-base regulation mechanisms in Japanese medaka (Oryzias latipes) ». Thesis, 2009. http://ndltd.ncl.edu.tw/handle/63622661244142152304.
Texte intégralRésumé :
碩士國立臺灣大學
漁業科學研究所
97
Euryhaline teleosts have to cope with the osmotic and ionic gradients of aquatic environments with diverse salinities, ion compositions, and pH values. Previous studies suggested that mitochondria-rich (MR) cells are specialized ionocytes, which are the main site responsible for ion regulation mechanisms in fish gills and embryonic skin. However, there are still many unclear issues of how transporters are functioning in fish ion regulation mechanism. The present study used Japanese medaka as a model to examine the roles of the related ion transporters in fish Na+ uptake and acid/base balance mechanisms. Three ion transporters (Na+/H+ exchanger 2/3, NHE2/3; and V-type H+-ATPase and two transcription factors (forkhead box transcription factor I 3, FOXI3; and glial cell missing 2, GCM2) related to ionocyte differentiation have been successfully cloned from Japanese medaka. Using double in situ hybridization/immunocytochemistry, Na+/K+-ATPase (NKA) and NHE were colocalized in MR cells, H+-ATPase (HA) was localized in a part of MR cells. In qRT-PCR experiments of adult medaka gills, after acclimation to acidic freshwater slc9a2 (NHE2), slc9a3 (NHE3) and atp6v1a (H+-ATPase) were up-regulated, while gcm2 was down-regulated; on the other hand, slc9a3, atp6v1a, and foxi3 were up-regulated during acclimation to low sodium water. Taken together, NHE and HA may play some roles in sodium uptake/acid-base regulation pathways in medaka, and the 2 transcriptional factors, foxi3 and gcm2, may participate in the ionocyte differentiation pathway.
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Lai, Yun-Wei, et 賴昀瑋. « Expression and regulation of gill ion transporters in Japanese medaka (Oryzias latipes) during SW acclimation ». Thesis, 2008. http://ndltd.ncl.edu.tw/handle/00784132707588480459.
Texte intégralRésumé :
碩士國立臺灣大學
生化科學研究所
96
Euryhaline teleosts have to cope with the fluctuating salinities of the environments in which they inhabit during their life time. In current model of gill salt scecretion of seawater- (SW) adaptated teleosts, mitochondria-rich cells (MR cells) play the major roles in the active ion secretion mechanism. The ion secretion mechanism in SW gill MR cells is achieved by the balsolateral Na+/K+-ATPase (NKA) and Na+/K+/2Cl- cotransporter (NKCC), and an apical membrane located cystic fibrosis transmembrane conductance regulator (CFTR). However, this model still lacks sufficient molecular evidences and leaves some controversies in the previous studies. The present study used Japanese medaka (Oryzias latipes, OL) as the model species to provide molecular and physiological evidence to support the roles of the relevant transporters in the current NaCl secretion model of SW type MR cells. The mortality experiment showed that OL enhanced their salinity adaptability after a pre-acclimation to SW and even after back to FW for 1 wk. This study examined the mRNA expression patterns of 8 transporter isoforms, and found that 3 NKA isoforms (atp1a1a.1, atp1b1a and atp1b1b), 1 NKCC (slc12a1a) and 1 CFTR (abcc7) mRNA levels were up-regulated during SW exposure, suggesting that these genes may play critical roles in salt secretion. Interestingly, the elevated mRNA levels of atp1b1b and slc12a2a in SW medaka were prolonged even after transfer back to FW for 1 wk, indicating that occurrence of a latent salt secretion mechanism of these 2 genes may be necessary for the subsequent salinity challenge as we mentioned above. Furthermore, atp1a1a.1, atp1b1b, slc12a2a and abcc7 were found to be co-expressed in the same MR cell, providing the first molecular evidence for their isoform-specific identities. The cell number of proliferation (by PH3 stainning) and apoptosis (by TUNEL assay) in gill MR cells showed that the gill cell’s turnover rate is faster in SW than in FW. In addition, olfoxi3 expression indicated the differentiation of MR cells during SW acclimation. NKCC and CFTR mRNA expression levels highly related to the number of SW-type MR cells. According to these results, we proposed that the differentiation of immature cells into SW-type MR cells can be accelerated to enhance NaCl secretion pathway in SW. In summary, several isoforms were isolated from gill MR cells of OL, and in vivo molecular evidences demonstrated their roles in iono/osmo-regulation and cell differentiation mechanisms in fish gills. This supports the notion that the expressions of these ion transporters correlate with the NaCl secretion function in MR cells, and also offer new insights into the studies on salinity tolerance in euryhaline teleosts.
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Lai, Yun-Wei. « Expression and regulation of gill ion transporters in Japanese medaka (Oryzias latipes) during SW acclimation ». 2008. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-0906200818092000.
Texte intégral
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Pan, Shu-Chin, et 潘素琴. « Induction of erythrocyte alterations in the Japanese Medaka , Oryzias latipes , by common pesticides（methomyl and butachlor） ». Thesis, 2005. http://ndltd.ncl.edu.tw/handle/99675268113602369052.
Texte intégralRésumé :
碩士國立臺灣海洋大學
環境生物與漁業科學學系
93
Abstract The purpose of this thesis is to investigate the toxic effects of two common pesticides ( insecticide and herbicide) on Japanese Medaka (Oryzias latipes). The micronucleus tests examined after 96 hours exposure of 3 different concentrations(1.0, 2.0, 3.0 ppm) of Methomyl and Butachlor, were studied in order to find out the risk of aquatic ecosystems due to the genotoxicity of pesticides. The results are summarized as follow: 1. The occurrence frequencies of micronucleus were slightly increased with dose- dependent response in methomyl experiments. 2. After exposure in common pesticides, erythrocytes were induced to cellular transformations: spherocytes, anisocytosis, echinocytes, vacuolation and haemolysis. 3. Nuclear abnormalities other than micronuclei were found such as notched nuclei, blebbed nuclei, diffused nuclear amterial and enucleated cells. Key words: Japanese Medaka (Oryzias latipes), pesticide, herbicide, micronucleus
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Hsu, Hao-Hsuan, et 徐浩軒. « Molecular and cellular mechanisms of functional modification of ionocytes during seawater acclimation in Japanese Medaka (Oryzias latipes) ». Thesis, 2010. http://ndltd.ncl.edu.tw/handle/61585340097251492220.
Texte intégralRésumé :
碩士國立臺灣大學
漁業科學研究所
98
Ionocytes, mitochondrion-rich cells (MRCs), are specialized epidermal cells which maintain ionic homeostasis of body fluid by actively taking up or excreting ions. The purpose of this study is to examine the regulatory mechanism of cell turnover and transporters expression of ionocytes in Japanese Medaka (Oryzias latipes) during seawater (SW) acclimation. Four types of ionocyte were identified in medaka embryo, the freshwater (FW) type were NHE cell, NCC cell and HA cell and only one SW type ionocyte. The NHE cells expressing apical Na+/H+ exchanger-3 (NHE3), cystic fibrosis conductance regulator (CFTR), and basolateral Na+/K+-ATPase (NKA) and constituted the majority of FW ionocytes. We also found that most of ionocytes were basolateral expressing NKCC1a and co-localized with NKA suggesting that they are belonged to NHE cell. The cell number of NCC cell expressing apical Na+/Cl- cotransporter (NCC) was in minority. Expressions of above-mentioned transporters were regulated in response to SW stimulation. NKCC1a signal was up-regulated and no apical NCC signal was found in SW-acclimated embryo. The gene expression of NHE3 (slc9a3) was down-regulated but CFTR (abcc7) was up-regulated in medaka gill during 24 hours SW transfer. In 2-days SW transfer medaka embryo, the density of NKA-immunoreactive ionocytes was not changed, but the cell size became larger. Ionocyte turnover was examined on medaka yolk sac membrane during short-term SW adaptation. P63 is an epidermal stem cell marker, immunocytochemistry of P63 showed no significant difference in the density of P63-cells after 1 hour and 7 hours SW transfer, but a little decrease showed after 1 day SW transfer. The result of TUNEL assay indicated that cell apoptosis was not significant at 12, 24 and 48 hours after SW transfer. By qRT-PCR, The expression of ionocyte differentiation marker foxi3 and gcm2 did not differ between FW and 24-hour SW transfer medaka gill. These results suggest that proliferation, differentiation and apoptosis of epidermal cells were not changed in short-term SW adaptation response. In mammalian, deleted in malignant brain tumor 1 (dmbt1) encodes Hensin which transform b-intercalated cell into a-intercalated cell in cortical collecting duct during systemic metabolic acidosis. In medaka, dmbt1 was also expressed in ionocytes. The protein expression of DMBT1 was down-regulated in 36 hours SW transfer embryo by immunocytochemistry. However, there was no difference in the gene expression of dmbt1 during 24 hours SW tranfer. Loss-of-function experiment showed higher mortality in dmbt1 morpholino-injected group than in control. Morpholino knock down accompany with SW transfer treatment showed obvious delay in the development of medaka morphant. We suggested dmbt1 might involve in transformation of ionocytes during SW acclimation. Taken together, we demonstrated a model of short-term SW adaptation in medaka which suggests that FW ionocytes directly transform to SW ionocytes and dmbt1 might involve in this process.
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Su, Yu-Ching, et 蘇于菁. « Using Japanese Medaka Primary Hepatic Cell Culture to Investigate the Effect of Wastewater on Expression of VTG ». Thesis, 2005. http://ndltd.ncl.edu.tw/handle/96165685744381733705.
Texte intégralRésumé :
碩士嘉南藥理科技大學
環境工程與科學系碩士班
93
In the last decade, much of savant efforts have been on studying endocrine-disrupting chemicals (EDCs), or commonly known as environmental hormones. These chemicals can imitate natural hormones and interfere not only the normal endocrinal, but also immune, reproductive and nerve systems in animals and humans. Some EDCs can be found in different water bodies or even in sewage-treatment works (STWs) effluents. The aims of this study were to investigate the effects of effluent from different sources on induction of vitellogenin (VTG) in the primary hepatocyte culture of Japanese medaka (Oryzias latipes) Because aquatic toxicology studies need to sacrifice a lot of animals, using cell culture can minimize the number of the animals used for different purposes. The medaka hepatocytes were cultivated in flasks with medium, and were exposed to 17-β estradiol and samples extracted from wastewater for 5days, 10days and 15days. The culture mediums were changed daily. VTG induction in hepatocytes was analyzed using Western blotting and quantified by the Bio-Rad’s image analysis system. The results showed that VTG induction by 17-β estradiol was dose-dependent after the 15-day exposure. Most of the influent samples from constructed wetlands could not induce vitellogenesis in cultured cells until exposed for 15 days. All of the effluent samples tested showed no VTG inducibility except those from An-Ping STW, and the responses were dependent with exposure time. Based on the results in this study, using Japanese medaka primary hepatic cell culture to investigate the effects of wastewater on induction of VTG was feasible, and at the same time, can be served as a alternative for in vivo study.
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Bauer, Anthony E. « Identification of Oil Sands Naphthenic Acid Structures and Their Associated Toxicity to Pimephales promelas and Oryzias latipes ». Thesis, 2013. http://hdl.handle.net/10012/7297.
Texte intégralRésumé :
The oil sands, located in north-eastern Alberta, are one of the largest deposits of oil worldwide. Because the Alberta Environmental Protection and Enhancement Act prohibits the release of oil sands process-affected material into the environment, industry is storing vast quantities of tailings on mine lease sites. The oil sands industry is currently accumulating tailings waste at a rate of >105 m3/day, for which reclamation strategies are being investigated. Naphthenic acids (NAs) have been identified as the most toxic component of oil sands tailings as they are considered acutely toxic to a variety of biota, and are therefore a target contaminant for tailings pond reclamation strategies. Current literature based on Microtox® assays (marine bacteria Vibrio fischeri) suggests that lower molecular weight NAs are more toxic than higher molecular weight NAs. The following thesis involves the utilization of NA fractions and their relative toxicities to determine if NA toxicity is related to NA molecular weight.
A previous study generated an oil sands-derived naphthenic acid extract (NAE), which was fractionated by distillation at stepped temperatures, yielding five fractions with increasing median molecular weights (Daltons). In the present study, the same extract and five fractions were utilized. To expand on the earlier characterization which involved a low resolution electrospray ionization mass spectrometry (ESI-MS), the whole extract and five fractions were analysed using electrospray ionization high-resolution mass spectrometry (ESI-HRMS) and synchronous fluorescence spectroscopy (SFS). Mean molecular weights were generated for each fraction, and an increase in molecular weight with increasing fraction number was confirmed. Respective mean Daltons and relative proportions for each fraction are as follows: 237 and 11.9 % (fraction 1), 240 and 32.3% (fraction 2), 257 and 33.4% (fraction 3), 308 and 16.8% (fraction 4), and 355 and 5.6% (fraction 5). When chemical analyses of fractions were compared, it was determined that structures contributing to increased molecular weight included increased cyclic structures (up to 7-ring structures), aromaticity (mono- and diaromatics), nitrogen, sulfur, and oxygen heteroatoms, and dihydroxy/dicarboxy compounds. In addition, characterization data suggested the presence of NAs exhibiting estrogenic structures.
Following chemical characterization, NA fractions were subject to embryo/larval bioassays using two fish species: Oryzias latipes (Japanese medaka) and Pimephales promelas (fathead minnow). Endpoints evaluated were mortality, time to hatch, hatch length, and abnormalities. Results suggest that relative NA fraction toxicity is not related to molecular weight, as no trend relating mean Dalton weight to toxicity was observed for any endpoint in both species. Acute toxicity data indicated differences between fractions as high as 2-fold, although results were species-dependent. Fraction 1 displayed the lowest potency (highest LC50) for both Japanese medaka (0.291 mM) and fathead minnow (0.159 mM). Fractions 3 and 2 for Japanese medaka (0.149 and 0.157 mM, respectively), and fractions 5 and 2 for fathead minnow (0.061 and 0.080 mM, respectively) displayed the greatest potencies for mortality (lowest LC50). When fraction LC50s for Japanese medaka were compared to the whole NAE (0.143 mM), the mid molecular weight fractions (fractions 2 and 3) appeared most similar to the whole NA. . In terms of relative toxicity and proportion, constituents in the mid molecular range fractions (2 and 3) likely represent greater risk compared to other fractions, and further chemical and toxicological characterization of constituents within these fractions is warranted particularly for long-chained, monocarboxylic acids, with low aromaticity.
Japanese medaka and fathead minnow varied in their sensitivity and their relative response to different fractions. In general, fathead minnow were more sensitive than Japanese medaka based on lower estimates of LC50 and threshold (growth) values in addition to the presence of developmental abnormalities (predominately yolk sac edema) associated with a few of the fractions. Compared to differences in toxicity between fractions for a given species (>2-fold for fathead minnow), there was more variability between species for a given fraction (> 3-fold for fraction 5). Also, the relative toxicity of fractions as indicated in the present study is contrary to the results generated using Vibrio fischeri for the same fractions. Thus, there is a need for multi- endpoint and species toxicity evaluations to assess the efficacy of remediation and reclamation options for reducing toxicity of oil sands tailings.
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Clark, Lia. « Competitive aggression in male and female Japanese medaka (Oryzias latipes) in relation to the operational sex resource ratio ». Thesis, 2009. http://spectrum.library.concordia.ca/976647/1/MR63260.pdf.
Texte intégralRésumé :
Because of the fundamental asymmetry between the sexes, males typically compete for access to females. Hence, most studies on intra-sexual mating aggression have focused only on males, and have shown increasing aggression with increasing operational sex ratio (OSR). However, one study documented a "dome-shaped" relationship between aggression and OSR, presumably because aggression became ineffective at high competitor densities as predicted by resource defence theory. The few studies that have investigated female intra-sexual aggression have used only a narrow range of OSR. The purpose of my study was to investigate the patterns of both male and female mating aggression over a broad range of OSR. I also compared how females competed for two different resources, food and mates. Male and female aggression initially increasing with OSR, peaked and then levelled off. For a given value of OSR, however, the rate of male aggression was higher than female aggression. The rate of male aggression was consistent with a dome-shaped pattern, whereas the rate of female aggression did not decrease at high levels of OSR. In contrast, courtship rates for both males and females decreased with increasing OSR in a linear manner. The pattern of male and female intra-sexual aggression were broadly consistent with the predictions of resource defence theory.
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Hirano, Chalinee. « Political information contests and the media's role in politics : a comparative analysis of the Thai and Japanese media ». Phd thesis, 2001. http://hdl.handle.net/1885/146044.
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Scarano, Louis John. « Evaluation of rodent peroxisome proliferators in two species of fish (rainbow trout ; Salmo gairdneri and Japanese medaka ; Oryzias latipes) ». 1992. https://scholarworks.umass.edu/dissertations/AAI9219493.
Texte intégralRésumé :
Rainbow trout (Salmo gairdneri) and Japanese medaka (Oryzias latipes) were exposed to three or four compounds, respectively, which have been shown to cause peroxisome proliferation in rodents. Trout were injected (intraperitoneally) daily for two weeks to the following chemicals and doses; the dimethylamine salt of 2,4-dichlorophenoxyacetic acid (DMA of 2,4-D) at 0, 2.5, 5 and 10 mg/kg/d, trichloroethylene (TCE) at 0, 10, 50 and 100 mg/kg/d or gemfibrozil at 0, 46, 87 and 152 mg/kg/d. Japanese medaka were exposed to di (2-ethylhexyl) phthalate (DEHP), 2,4-D (DMA) or gemfibrozil in water for two weeks in a static renewal system. Nominal doses used were 0, 90, 180 and 360 ppb, 0, 50, 100 and 200 ppm and 0, 1.25, 2.5 and 5 ppm for DEHP, 2,4-D and gemfibrozil, respectively. Medaka were also exposed to TCE for 16 hours in a closed system at doses of 0, 25 and 50 ppm. Peroxisome proliferation was assessed by measuring fatty acyl-CoA oxidase (FAO) activity and relative percent increase in peroxisomal bifunctional enzyme (PBE); enzymes which are involved in peroxisomal beta-oxidation. In addition, changes in liver weight/body weight ratios were measured. Results indicate that a mild peroxisome proliferative response was observed in rainbow trout exposed to gemfibrozil (significant increase in FAO activity at all three dose levels and a significant increase in liver weight/body weight ratios at the highest dose level only). There was no difference between control and treated groups in the trout exposed to 2,4-D or TCE. In the medaka experiments, a marginal response was observed in the gemfibrozil experiment (significant increase in PBE at the highest dose level and a non-significant increase in FAO activity in the mid- and high-dose groups). There were no significant, treatment related differences between control and treated fish in the TCE, 2,4-D and DEHP medaka experiments. It was concluded that fish may not be a sensitive model to screen chemicals for their ability to induce peroxisome proliferation.
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Robb, Stacey E. « Aggression, monopolization and growth depensation within groups of Japanese medaka (Oryzias latipes) : interactions between the temporal and spatial clumping of food ». Thesis, 1996. http://spectrum.library.concordia.ca/244/1/MQ25980.pdf.
Texte intégralRésumé :
Resource defence theory predicts that defence and monopolization increase as the spatial clumping, spatial predictability, and temporal predictability of resources increase and as temporal clumping of resources decrease. Previous tests of resource defence theory have manipulated only one aspect of resource distribution at a time. I tested whether there was an interaction between the effects of the spatial and temporal clumping of food by allowing groups of ten Japanese medaka (Oryzias latipes) to compete, over a 4 week period, for food in a 2 x 2 factorial design. As expected, the intensity of aggression increased as the spatial clumping of food increased and as the temporal clumping of food decreased. However, there was also a significant interaction between the effects of the spatial and temporal clumping of food on the intensity of aggression and total aggression. The number of fish in the feeding patch, a measure of monopolization, decreased as the spatial clumping of food increased, but was not affected by the temporal clumping of food. Although measures of aggression were high in the economically defendable trials, there was no apparent cost to resource defence, since mean growth rates did not differ significantly among treatments. Growth depensation increased over time, but was not influenced by the temporal or spatial distribution of food. The power of my experiment to detect a significant effect of food distribution on growth depensation was low. Therefore, although I failed to reject the null hypothesis, I cannot confidently conclude that the spatial and temporal clumping of food have no effect on the magnitude of growth depensation. My results suggest that care must be taken when extrapolating the results of single factor experiments to multi-factor or field situations.
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SHOJI, OKADA, et 岡田庄治. « On the medial deletion of the third-division rhyme words in Go-on of Sino-Japanese and colloquial sound of Southern Min ». Thesis, 2009. http://ndltd.ncl.edu.tw/handle/kjvf2v.
Texte intégralRésumé :
碩士銘傳大學
應用日語學系碩士班
97
In pronunciation of Chinese characters, Go-on of Sino-Japanese closely resembles the colloquial sound form of Southern Min. Especially in the third division rhyme words that should be palatalized sound, both have many non-palatalized sound words. This thesis researched the medial deletion in the third-division rhyme words from a viewpoint of the traditional Chinese phonology and clarified the common part and the different part in both languages. In Southern Min, most of the colloquial words in the new stratum have palatalized sound, but the words in the old stratum have non-palatalized sound. In Go-on of Sino-Japanese, the medial deletion is decided by the position of main vowels and the position relations between onsets and finals. The conditions of the medial deletion in both languages are not same, but I applied the conclusion of this study to the pronunciation rule of Go-on, and showed the corresponding rule of the pronunciation of colloquial words in Southern min and Go-on of Sino-Japanese. It would be my honor to be a help of studying Sino-Japanese for Taiwanese students through this research.
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Bryant, Michael J. « Competition for food and variation of fitness in groups of female Japanese medaka (Oryzias latipes) depend on the synchrony of food arrival ». Thesis, 1993. http://spectrum.library.concordia.ca/2765/1/MM87299.pdf.
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(10724124), Elizabeth B. Allmon. « Cardiotoxic effects of polycyclic aromatic hydrocarbons and abiotic stressors in early life stage estuarine teleosts ». Thesis, 2021.
Trouver le texte intégralRésumé :
Following the 2010 Deepwater Horizon oil spill, extensive research has been conducted on the toxicity of oil and polycyclic aromatic hydrocarbons (PAHs) in the aquatic environment. The location and timing of the Deepwater Horizon surface slick coincided with the spawning seasons of many important pelagic and estuarine fish species. As such, there has been particular emphasis placed on the effects of PAHs on sensitive life history stages in fish, such as the embryonic and larval periods. Additionally, the spill occurred throughout the spring and summer months which, in estuaries, are marked by regular fluctuations in abiotic environmental factors such as dissolved oxygen, salinity, and temperature. Until recently, there has been little work done to elucidate the combined effects that PAHs from oil spills and adverse environmental conditions (hypoxia, increased salinity, and elevated temperatures).
Work presented in this dissertation uses next generation sequencing technology (RNA Seq) to determine differential gene expression in larval estuarine teleosts following exposure to adverse environmental conditions and PAHs. Downstream canonical pathway and toxicological function analysis were then applied to the identified differentially expressed genes (DEGs) to predict cardiotoxic responses at the organismal level. To verify the predicted responses, a phenotypic anchoring study was conducted and identified a cardiotoxic phenotype (pericardial edema) and reduced cardiac output in embryos exposed to oil. Finally, the mechano-genetic interplay governing the morphological development of the teleost heart was investigated and correlations between developmental gene expression and blood flow forces within the cardiovascular system were identified.