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Articles de revues sur le sujet « Hydrogen Peroxide Biosensor »

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Auteur : Grafiati
Publié le 14 mars 2023

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1

Baccar, H., T. Ktari et A. Abdelghani. « Functionalized Palladium Nanoparticles for Hydrogen Peroxide Biosensor ». International Journal of Electrochemistry 2011 (2011) : 1–4. http://dx.doi.org/10.4061/2011/603257.

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We present a comparison between two biosensors for hydrogen peroxide (H2O2) detection. The first biosensor was developed by the immobilization of Horseradish Peroxidase (HRP) enzyme on thiol-modified gold electrode. The second biosensor was developed by the immobilization of cysteamine functionalizing palladium nanoparticles on modified gold surface. The amino groups can be activated with glutaraldehyde for horseradish peroxidase immobilization. The detection of hydrogen peroxide was successfully observed in PBS for both biosensors using the cyclic voltammetry and the chronoamperometry techniques. The results show that the limit detection depends on the large surface-to-volume ratio attained with palladium nanoparticles. The second biosensor presents a better detection limit of 7.5 μM in comparison with the first one which is equal to 75 μM.
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2

Valencia, Germán Ayala, Luci Cristina de Oliveira Vercik et Andrés Vercik. « A new conductometric biosensor based on horseradish peroxidase immobilized on chitosan and chitosan/gold nanoparticle films ». Journal of Polymer Engineering 34, no 7 (1 septembre 2014) : 633–38. http://dx.doi.org/10.1515/polyeng-2014-0072.

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Abstract A new conductometric biosensor was developed and characterized; the biosensor was based on horseradish peroxidase that was deposited in chitosan and chitosan/AuNPs films. The biosensors were characterized by scanning electron microscopy and current-voltage curves. Current-voltage curves in biosensors showed that the electrical conductivity and bistability in biosensors can be modulated by horseradish peroxidase. Horseradish peroxidase catalyzed the reduction of H2 O2 to H2 O with the oxidation of the prosthetic group (Fe3+) in the enzyme to Fe4+=O. Conductometric signal in the biosensors increased with the gradual increase of H2 O2 concentration, and it was due to the H2 O2 reduction. Linear hydrogen peroxide detection was observed for a concentration between 0 and 15 mm. The results proved that these biosensors could have promising industrial applications, due to its rapid and sensitive H2 O2 detection.
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Baccar, Zouhair M., et Imène Hafaiedh. « Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application ». International Journal of Electrochemistry 2011 (2011) : 1–5. http://dx.doi.org/10.4061/2011/934893.

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We present a new biosensor for hydrogen peroxide (H2O2) detection. The biosensor was based on the immobilization of horseradish peroxidase (HRP) enzyme on layered double hydroxides- (LDH-) modified gold surface. The hydrotalcite LDH (Mg2Al) was prepared by coprecipitation in constant pH and in ambient temperature. The immobilization of the peroxidase on layered hybrid materials was realized via electrostatic adsorption autoassembly process. The detection of hydrogen peroxide was successfully observed in PBS buffer with cyclic voltammetry and the chronoamperometry techniques. A limit detection of 9 μM of H2O2was obtained with a good reproducibility. We investigate the sensitivity of our developed biosensor for H2O2detection in raw milk.
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4

Zhao, Dong Jiao, Yan Hong Chen, Chen Di Tu, Yao Fang Xuan et Feng Na Xi. « Construction of Reagentless Biosensor Based on Self-Assembly and Electrodeposition for Determination of Hydrogen Peroxide ». Advanced Materials Research 441 (janvier 2012) : 442–46. http://dx.doi.org/10.4028/www.scientific.net/amr.441.442.

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A general methodology to prepare reagentless biosensor was developed based on self-assembly and electrodeposition. Redox active inorganic multilayers consisting of copper hexacyanoferrate (CuHCF) multilayers were formed by successive self-assembly. A simple and controllable electrodeposition approach was established for one-step fabrication of chitosan-enzyme layer on CuHCF modified electrode. Horseradish peroxidase was selected as the model enzyme. With CuHCF as the electroactive mediator, the developed reagentless biosensor exhibited a fast amperometric response for the determination of hydrogen peroxide (H2O2). The linear response ranged from 1.4 × 10-5 to 2.0 × 10-4 M with a detection limit of 1.2 × 10-6 M. The biosensor exhibited high reproducibility and long-time storage stability. The proposed methodology could serve as a versatile platform for fabrication of electrochemical biosensors.
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Kafi, A. K. M., Dong-Yun Lee, Sang-Hyun Park et Young-Soo Kwon. « A Hydrogen Peroxide Biosensor Based on Peroxidase Activity of Hemoglobin in Polymeric Film ». Journal of Nanoscience and Nanotechnology 7, no 11 (1 novembre 2007) : 4005–8. http://dx.doi.org/10.1166/jnn.2007.095.

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A Hydrogen peroxide (H2O2) biosensor, based on hemoglobin (Hb) and ortho-phenylenediamine (o-PD) gold electrode, was fabricated. Hb was immobilized onto the electrode surface by electrochemical polymerize method with o-PD. The designed biosensor showed a well defined redox peak which was attributed to the direct electrochemical response of Hb. The immobilized Hb exhibited an excellent electrocatalytical response to the reduction of hydrogen peroxide, enabling the sensitivity determination of H2O2. Factors and performances such as pH, potential, influencing the designed biosensor, were studied carefully. The amperometric detection of H2O2 was carried out at −300 mV in phosphate buffer solution (PBS) (0.1 M) with pH 6.0. This biosensor showed a fast amperometric response (less then 5 s) to H2O2. The levels of the (Relative standard deviation) RSDs (<3 5%) for the entire analyses reflected a highly reproducible sensor performance. Using the optimized conditions, the detection limit of the biosensor was 1 × 10−7 M and linear range was from 5 × 10−6 to 1.25 × 10−4 M. In addition, this sensor showed long term stability and good sensitivity.
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Nguyen Duc, Nghia, Chinh Huynh Dang, Hoang Tran Vinh et Vu Dao Hong. « Peroxidase-like activity of Fe3O4/carbon core-shell nanostructured : effects of carbon shell thickness for application to glucose biosensor ». Vietnam Journal of Catalysis and Adsorption 10, no 2 (30 juillet 2021) : 109–13. http://dx.doi.org/10.51316/jca.2021.038.

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In this study, we present a protocol for synthesis of carbon coated Fe3O4 nanoparticles with core-shell structured nanocomposite (FeC) following a two steps approach. The peroxidase-like acitivity of the synthesized FeC nanocomposite has been evaluated towards replacing of the horseradish peroxidase enzyme (HRP) in hydrogen peroxide enzymatic biosensor. In which, FeC has catalyzed for a redox reaction 5,5'-tetramethylbenzidine (TMB) and H2O2 to produce oxidized state of TMB with as a blue color. Results exhibited that FeC has a high catalytic activity accepting for fabrication of a high selectivity hydrogen peroxide (H2O2) colorimetric sensor with low detection of limit (LoD) of 0.02 mM H2O2. Based on this finding, we have used FeC and combined with glucose oxidase (GOx) enzyme to construct a new colorimetric glucose biosensor with high selectivity.
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Chmayssem, Ayman, Ibrahim Shalayel, Stéphane Marinesco et Abdelkader Zebda. « Investigation of GOx Stability in a Chitosan Matrix : Applications for Enzymatic Electrodes ». Sensors 23, no 1 (1 janvier 2023) : 465. http://dx.doi.org/10.3390/s23010465.

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In this study, we designed a new biosensing membrane for the development of an electrochemical glucose biosensor. To proceed, we used a chitosan-based hydrogel that entraps glucose oxidase enzyme (GOx), and we crosslinked the whole matrix using glutaraldehyde, which is known for its quick and reactive crosslinking behavior. Then, the stability of the designed biosensors was investigated over time, according to different storage conditions (in PBS solution at temperatures of 4 °C and 37 °C and in the presence or absence of glucose). In some specific conditions, we found that our biosensor is capable of maintaining its stability for more than six months of storage. We also included catalase to protect the biosensing membranes from the enzymatic reaction by-products (e.g., hydrogen peroxide). This design protects the biocatalytic activity of GOx and enhances the lifetime of the biosensor.
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8

Zhang, Yunfei, Tingting Lin, Yi Shen et Hongying Li. « A High-Performance Self-Supporting Electrochemical Biosensor to Detect Aflatoxin B1 ». Biosensors 12, no 10 (20 octobre 2022) : 897. http://dx.doi.org/10.3390/bios12100897.

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High-performance electrochemical biosensors for the rapid detection of aflatoxin B1 (AFB1) are urgently required in the food industry. Herein, a multi-scaled electrochemical biosensor was fabricated by assembling carboxylated polystyrene nanospheres, an aptamer and horseradish peroxidase into a free-standing carbon nanofiber/carbon felt support. The resulting electrochemical biosensor possessed an exceptional performance, owing to the unique structures as well as the synergistic effects of the components. The 3D porous carbon nanofiber/carbon felt support served as an ideal substrate, owing to the excellent conductivity and facile diffusion of the reactants. The integration of carboxylated polystyrene nanospheres with horseradish peroxidase was employed as a signal amplification probe to enhance the electrochemical responses via catalyzing the decomposition of hydrogen peroxide. With the aid of the aptamer, the prepared sensors could quantitatively detect AFB1 in wine and soy sauce samples via differential pulse voltammetry. The recovery rates of AFB1 in the samples were between 87.53% and 106.71%. The limit of detection of the biosensors was 0.016 pg mL−1. The electrochemical biosensors also had excellent sensitivity, reproducibility, specificity and stability. The synthetic strategy reported in this work could pave a new route to fabricate high-performance electrochemical biosensors for the detection of mycotoxins.
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9

Gurbanova, Lala. « Sensors for analysis of hydrogen peroxide ». Scientific Bulletin 3 (2020) : 169–74. http://dx.doi.org/10.54414/bgif9220.

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The physico-chemical properties of new type catalase sensors, the so-called biomimetic sensors modulating some of catalase biosensor functions were investigated. These sensors have technological advantages ever their biological analogs due to the properties usually attributed to chemical sensors. The development electrochemical system stands in between bio- and chemical sensors.
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10

Karunakaran, Chandran, Murugesan Karthikeyan, Marimuthu Dhinesh Kumar, Ganesan Kaniraja et Kalpana Bhargava. « Electrochemical Biosensors for Point of care Applications ». Defence Science Journal 70, no 5 (8 octobre 2020) : 549–56. http://dx.doi.org/10.14429/dsj.70.16359.

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Biosensor refers to powerful and innovative analytical tool involving biological sensing element and transducer with broad range of applications, such as diagnosis, drug discovery, biomedicine, food safety and processing, environmental monitoring, security and defense. Recent advances in the field of biotechnology, microelectronics, and nanotechnology have improved the development of biosensors. Glucometers utilizing the electrochemical determination of oxygen or hydrogen peroxide employing immobilised glucose oxidase electrode seeded the discovery and development of biosensors. Molecular recognition based on geometry and forces of interaction play an important role in the biosensor development. The advent of nanotechnology led to highly efficient and sensitive biosensors. They also provide an effective immobilisation matrix for the various bioreceptors. Enzymatic and their mimetic (metalloporphyrin)-based biosensors for reactive oxygen, nitrogen species and cytochrome c will also be discussed. The role of antibodies and their applications in immunosensors development for cytochrome c and superoxide dismutase will be highlighted. The electrochemical biosensors are less expensive, miniaturised and used for point-of-care applications. Further, the fabrication of labVIEW based virtual biosensor instrumentation and microcontroller based portable biosensor for wide variety of applications also devices will be presented.
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Ariffin, Eda Yuhana, Nik Nurhanan Nik Mansor, Eka Safitri, Yook Heng Lee et Nurul Izzaty Hassan. « A Hydrogen Peroxide Biosensor from Horseradish peroxidase Immobilization onto Acrylic Microspheres ». Sains Malaysiana 48, no 7 (31 juillet 2019) : 1409–16. http://dx.doi.org/10.17576/jsm-2019-4807-09.

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12

Navas Dı́az, A., M. C. Ramos Peinado et M. C. Torijas Minguez. « Sol–gel horseradish peroxidase biosensor for hydrogen peroxide detection by chemiluminescence ». Analytica Chimica Acta 363, no 2-3 (mai 1998) : 221–27. http://dx.doi.org/10.1016/s0003-2670(98)00080-4.

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13

Morales, A., F. Céspedes, J. Mũnoz, E. Martínez-Fábregas et S. Alegret. « Hydrogen peroxide amperometric biosensor based on a peroxidase-graphite-epoxy biocomposite ». Analytica Chimica Acta 332, no 2-3 (octobre 1996) : 131–38. http://dx.doi.org/10.1016/0003-2670(96)82793-0.

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14

Uygun, Hilmiye Deniz Ertugrul, Nihat Tinkilic, Azade Attar et Ibrahim Isildak. « Development of Potentiometric Lactate Biosensor Based on Composite pH Sensor ». Journal of New Materials for Electrochemical Systems 19, no 3 (20 septembre 2016) : 151–56. http://dx.doi.org/10.14447/jnmes.v19i3.313.

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In this study, a micro-sized lactate sensitive biosensor based on polyvinylchloride, quinhydrone and graphite composite pH sensing platform was developed. Lactate oxidase was immobilized on the composite layer as the biorecognition element. Transformation reaction of lactate to pyruvate and hydrogen peroxide was the basis of this biosensor system. In the reaction, hydrogen peroxide undergoes to give hydronium ions into solution, and the pH sensitive membrane detects the adjunct hydronium ions potentiometrically. The surface of lactate biosensor based composite pH sensing matrice was first examined for electrochemical elucidation by using cyclic voltammetry and electrochemical impedance spectroscopy. A linear response in concentration range from 5x10-5 to 1x10-1 mol/L was obtained with a detec-tion limit of 2x10-5 mol/L. The lactate biosensor developed was successfully applied for highly precise and efficient determination of lactate in food preparations. The biosensor exhibited a fast response time (10 s), had good stability, and had an extended lifetime.
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15

Chen, Lu, Meng Shan Lin, Minoru Hara et G. A. Rechnitz. « Kohlrabi-Based Amperometric Biosensor for Hydrogen Peroxide Measurement ». Analytical Letters 24, no 1 (janvier 1991) : 1–14. http://dx.doi.org/10.1080/00032719108052879.

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Lupu, Alina, Patricia Lisboa, Andrea Valsesia, Pascal Colpo et François Rossi. « Hydrogen peroxide detection nanosensor array for biosensor development ». Sensors and Actuators B : Chemical 137, no 1 (mars 2009) : 56–61. http://dx.doi.org/10.1016/j.snb.2008.10.004.

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Wang, Peng, Shengqi Li et Jinqing Kan. « A hydrogen peroxide biosensor based on polyaniline/FTO ». Sensors and Actuators B : Chemical 137, no 2 (2 avril 2009) : 662–68. http://dx.doi.org/10.1016/j.snb.2008.12.055.

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18

Tai, G. Y., M. L. Wen et C. Y. Wang. « Bacteria-Based Biosensor for Determination of Hydrogen Peroxide ». Microchemical Journal 53, no 2 (février 1996) : 152–57. http://dx.doi.org/10.1006/mchj.1996.0021.

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19

Li, Meng, Jing Wu, Haiping Su, Yan Tu, Yazhuo Shang, Yifan He et Honglai Liu. « Ionic Liquid-Polypyrrole-Gold Composites as Enhanced Enzyme Immobilization Platforms for Hydrogen Peroxide Sensing ». Sensors 19, no 3 (3 février 2019) : 640. http://dx.doi.org/10.3390/s19030640.

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In this work, three different aqueous solutions containing imidazole-based ILs with different alkyl chain lengths ([Cnmim]Br, n = 2, 6, 12) were adopted as the medium for the synthesis of ionic liquid-polypyrrole (IL-PPy) composites. Herein, the ILs undertook the roles of the pyrrole solvent, the media for emulsion polymerization of PPy and PPy dopants, respectively. The electrochemical performances of the three IL-PPy composites on a glassy carbon electrode (GCE) were investigated by electrochemical experiments, which indicated that [C12mim]Br-PPy (C12-PPy) composites displayed better electrochemical performance due to their larger surface area and firmer immobilization on the GCE. Further, C12-PPy/GCE were decorated with Au microparticles by electrodeposition that can not only increase the conductivity, but also immobilize sufficient biomolecules on the electrode. Then, the obtained C12-PPy-Au/GCE with outstanding electrochemical performance was employed as a horseradish peroxidase (HRP) immobilization platform to fabricate a novel C12-PPy-Au-HRP/GCE biosensor for H2O2 detection. The results showed that the prepared C12-PPy-Au-HRP/GCE biosensor exhibited high sensitivity, fast response, and a wide detection range as well as low detection limit towards H2O2. This work not only provides an outstanding biomolecule immobilization matrix for the fabrication of highly sensitive biosensors, but also advances the understanding of the roles of ILs in improving the electrochemical performance of biosensors.
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Khan, Naveed Ahmad, Fahad Sameer Alshammari, Carlos Andrés Tavera Romero, Muhammad Sulaiman et Ghaylen Laouini. « Mathematical Analysis of Reaction–Diffusion Equations Modeling the Michaelis–Menten Kinetics in a Micro-Disk Biosensor ». Molecules 26, no 23 (2 décembre 2021) : 7310. http://dx.doi.org/10.3390/molecules26237310.

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In this study, we have investigated the mathematical model of an immobilized enzyme system that follows the Michaelis–Menten (MM) kinetics for a micro-disk biosensor. The film reaction model under steady state conditions is transformed into a couple differential equations which are based on dimensionless concentration of hydrogen peroxide with enzyme reaction (H) and substrate (S) within the biosensor. The model is based on a reaction–diffusion equation which contains highly non-linear terms related to MM kinetics of the enzymatic reaction. Further, to calculate the effect of variations in parameters on the dimensionless concentration of substrate and hydrogen peroxide, we have strengthened the computational ability of neural network (NN) architecture by using a backpropagated Levenberg–Marquardt training (LMT) algorithm. NNs–LMT algorithm is a supervised machine learning for which the initial data set is generated by using MATLAB built in function known as “pdex4”. Furthermore, the data set is validated by the processing of the NNs–LMT algorithm to find the approximate solutions for different scenarios and cases of mathematical model of micro-disk biosensors. Absolute errors, curve fitting, error histograms, regression and complexity analysis further validate the accuracy and robustness of the technique.
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Milardović, Stjepan, Zorana Grabarić, Mihael Tkalčec et Vlatko Rumenjak. « Determination of Oxalate in Urine, Using an Amperometric Biosensor with Oxalate Oxidase Immobilized on the Surface of a Chromium Hexacyanoferrate-Modified Graphite Electrode ». Journal of AOAC INTERNATIONAL 83, no 5 (1 septembre 2000) : 1212–17. http://dx.doi.org/10.1093/jaoac/83.5.1212.

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Abstract A novel enzymatic amperometric method is described for the determination of oxalic acid in urine. An amperometric biosensor was made by immobilizing oxalate oxidase on the surface of a chromium(III) hexacyanoferrate-modified graphite electrode by using a bovine serum albumin and glutaraldehyde cross-linking procedure. The enzyme biocatalyzes oxalate decomposition in the presence of oxygen into carbon dioxide and hydrogen peroxide. The oxalate concentration, which is proportional to the amount of hydrogen peroxide, was determined amperometrically by measuring the current resulting in the reduction of hydrogen peroxide at a very low working potential (0.05 V versus the Hg | Hg2Cl2 | 3M KCl electrode), which minimized the influence of the possible interferences present in human urine. All experiments were performed with succinic buffer, pH 3.8, containing 0.1M KCl and 5.4mM ethylenediaminetetraacetic acid. In an aqueous solution of pure oxalic acid, the biosensor showed good linearity in a concentration range of 2.5–100μM without the use of a dialysis membrane. For untreated urine samples, a high correlation (R2 = 0.9949) was obtained between oxalate concentrations added to urine samples and oxalate recoveries calculated for determinations with the described oxalate biosensor.
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O'Riordan, Saidhbhe L., Kelly Mc Laughlin et John P. Lowry. « In vitro physiological performance factors of a catalase-based biosensor for real-time electrochemical detection of brain hydrogen peroxide in freely-moving animals ». Analytical Methods 8, no 42 (2016) : 7614–22. http://dx.doi.org/10.1039/c6ay02190e.

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Cui, Han, Zhao Hao Wang, Qi Jin Wan et Nian Jun Yang. « Self-Assembled Au Electrode for Direct Electrochemistry of Horseradish Peroxidase and Detection of Hydrogen Peroxide ». Advanced Materials Research 704 (juin 2013) : 72–76. http://dx.doi.org/10.4028/www.scientific.net/amr.704.72.

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The H2O2 biosensor was prepared by continuous processes: 2,3-dimercaptosuccinic acid (DMSA) self-assembly monolayers gold nanoparticles (AuNPs) film by electrodepositioncovalent immobilization of horseradish peroxidase (HRP). In pH 7.2 phosphoric buffer solution (PBS), the HRP-AuNPs-DMSA-Au electrode exhibited a pair of well-defined cyclic voltammetric peaks in H2O2 solution, and the oxidation peak is about +0.4V versus saturated calomel electrode (SCE). The resulting substrates were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The biosensor exhibited remarkable sensitivity towards H2O2 and a wide dynamic range of 2-800 μM. The electrode retained 90% of its initial activity after 30 days of storage at 4°C.
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Fois, Marco, Paola Arrigo, Andrea Bacciu, Patrizia Monti, Salvatore Marceddu, Gaia Rocchitta et Pier Andrea Serra. « The Presence of Polysaccharides, Glycerol, and Polyethyleneimine in Hydrogel Enhances the Performance of the Glucose Biosensor ». Biosensors 9, no 3 (30 juillet 2019) : 95. http://dx.doi.org/10.3390/bios9030095.

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The use of amperometric biosensors has attracted particular attention in recent years, both from researchers and from companies, as they have proven to be low-cost, reliable, and very sensitive devices, with a wide range of uses in different matrices. The continuous development of amperometric biosensors, since their use involves an enzyme, is specifically aimed at keeping and increasing the catalytic properties of the loaded protein, so as to be able to use the same device over time. The present study aimed to investigate the impact of glycerol and polysaccharides, in the presence of polycationic substances to constitute a hydrogel, in enhancing the enzymatic and analytic performance of a glucose biosensor. Initially, it was possible to verify how the deposition of the starch-based hydrogel, in addition to allowing the electropolymerization of the poly(p-phenylenediamine) polymer and the maintenance of its ability to shield the ascorbic acid, did not substantially limit the permeability towards hydrogen peroxide. Moreover, different biosensor designs, loading a mixture containing all the components (alone or in combination) and the enzyme, were tested in order to evaluate the changes of the apparent enzyme kinetic parameters, such as VMAX and KM, and analytical response in terms of Linear Region Slope, highlighting how the presence of all components (starch, glycerol, and polyethyleneimine) were able to substantially enhance the performance of the biosensors. The surface analysis of the biosensors was performed by scanning electron microscope (SEM). More, it was shown that the same performances were kept unchanged for seven days, proving the suitability of this biosensor design for short- and mid-term use.
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Wang, Ping, Li Hua Cheng et Ru Jin Zhou. « Study on the Photoelectric Properties of CdSe/MS Composite Film and its Potential Application in Biosensor Field ». Advanced Materials Research 560-561 (août 2012) : 688–93. http://dx.doi.org/10.4028/www.scientific.net/amr.560-561.688.

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CdSe nanocrystals were prepared within a template of mesoporous silica (MS) spheres via chemical reaction. The photocurrent of CdSe/MS composite under UV illumination was distinctly higher than that in the dark. The horseradish peroxidase (HRP) was immobilized on the surface of the composite particles. The HRP/CdSe/MS composite film toward the reduction of hydrogen peroxide (H2O2) had stronger response signal under UV illumination than that in the dark. The electrons photo-generated from CdSe and the electrons provided by electrode can occur simultaneously to catalyze the reduction of H2O2. The holes photo-generated by CdSe are trapped at the interface between CdSe and pore walls of MS film. The results show that CdSe/MS composite film has good photoelectric properties and potential application on immobilization of horseradish peroxidase for hydrogen peroxide monitoring.
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Alpeeva, Inna S., Mihaela Niculescu-Nistor, Jaime Castillo Leon, Elisabeth Csöregi et Ivan Yu Sakharov. « Palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring ». Biosensors and Bioelectronics 21, no 5 (novembre 2005) : 742–48. http://dx.doi.org/10.1016/j.bios.2005.01.008.

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Tang, Jilin, Bingquan Wang, Zhengyan Wu, Xiaojun Han, Shaojun Dong et Erkang Wang. « Lipid membrane immobilized horseradish peroxidase biosensor for amperometric determination of hydrogen peroxide ». Biosensors and Bioelectronics 18, no 7 (juillet 2003) : 867–72. http://dx.doi.org/10.1016/s0956-5663(02)00148-3.

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Ren, Qiong-Qiong, Fen Yang, Wu Ren, Chang Wang, Wen-Shuai Jiang, Zong-Ya Zhao, Jun Chen, Xuan-Yi Lu et Yi Yu. « Amperometric Biosensor Based on Coimmobilization of Multiwalled Carbon Nanotubes and Horseradish Peroxidase-Gold Nanocluster Bioconjugates for Detecting H2O2 ». Journal of Nanomaterials 2020 (11 juillet 2020) : 1–6. http://dx.doi.org/10.1155/2020/9627697.

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An enzyme-based amperometric biosensor was fabricated for detecting hydrogen peroxide (H2O2). Horseradish peroxidase (HRP) was modified using functionalized fluorescent gold nanoclusters (AuNCs) via biomineralization. HRP-AuNCs were successfully immobilized on multiwalled carbon nanotube- (MWCNT-) coated carbon fiber ultramicroelectrodes (CFUMEs). The AuNCs, which act as molecular electric wires, effectively promote the electron transfer between the enzyme active center and the electrode. Additionally, the HRP conjugated with the AuNCs retains its biological activity, which enables the catalytic reaction of H2O2. The HRP-AuNCs/MWCNTs/CFUMEs have been proven as excellent amperometric sensors for H2O2. The sensitivity of the H2O2 biosensor is 3.0×10−4 A/M, and the detection limit is estimated to be 443 nM. Furthermore, the biosensor exhibited long-term stability and good reproducibility. Moreover, the biosensor has been tested by determining the H2O2 concentration in calf serum samples.
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O'Riordan, Saidhbhe L., et John P. Lowry. « In vivo characterisation of a catalase-based biosensor for real-time electrochemical monitoring of brain hydrogen peroxide in freely-moving animals ». Analytical Methods 9, no 8 (2017) : 1253–64. http://dx.doi.org/10.1039/c6ay03066a.

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Zhang, Min, Fa Liang Cheng, Zhi Quan Cai et Mei Qiong Chen. « A New Hydrogen Peroxide Biosensor Based on Hrp/Ag Nanowires/Glassy Carbon Electrode ». Applied Mechanics and Materials 110-116 (octobre 2011) : 577–84. http://dx.doi.org/10.4028/www.scientific.net/amm.110-116.577.

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An amperometric biosensor for H2O2 detection was developed with immobilization of Horseradish Peroxidase (HRP) on Ag nanowires (AgNWs). Ag nanowires (AgNWs) have been prepared by the reductive deposition method and characterized by the scanning electron microscopy (SEM) and the energy dispersive X-ray (EDX). The electrochemical performance of the HRP/AgNWs/GC electrode was investigated by cyclic voltammetry (CV) and chronoamperometry and the use of Ag nanowires led to an efficient enzyme loading, and also provided an increased surface area for sensing the reaction, showing high electrocatalytic activity towards the reduction of H2O2. Under the optimized conditions, the response of the biosensor towards H2O2 was investigated by chronoamperometry. The biosensor exhibited excellent sensitivity (the detection limit was down to 0.005 mM), fast response time (15 sec). Moreover, the biosensor had long-time stability and good reproducibility.
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Мачигов, Э. А., Д. А. Свиридова et С. К. Абилев. « The study of paraquat genotoxicity by bacterial lux-biosensors ». Nauchno-prakticheskii zhurnal «Medicinskaia genetika», no 9(218) (30 septembre 2020) : 63–64. http://dx.doi.org/10.25557/2073-7998.2020.09.63-64.

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Показано, что паракват (1,1-диметил-4,4дипиридилия дихлорид) индуцирует люминесценцию биосенсоров, несущих промоторы генов soxS и katG, специфично реагируюших на супероксид-анион радикал и перекись водорода, соответственно. Антиоксиданты глутатион и ацетилцистеин снижали уровень как люминесценции биосенсоров, так и разрывов ДНК в бактериальных клетках. It has been shown that paraquat (1,1-dimethyl-4,4 dipyridylium dichloride) induces luminescence of biosensors carrying the promoters of the soxS and katG genes that specifically react to the superoxide anion radical and hydrogen peroxide. The antioxidants glutathione and acetylcysteine reduced the level of biosensor luminescence and DNA breaks.
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Zhang, Wei-Yi, Hao Zhang et Feng-Qing Yang. « An Economical and Portable Paper-Based Colorimetric Sensor for the Determination of Hydrogen Peroxide-Related Biomarkers ». Chemosensors 10, no 8 (17 août 2022) : 335. http://dx.doi.org/10.3390/chemosensors10080335.

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In this study, a paper-based sensor was developed for the detection of hydrogen-peroxide-related biomarkers, with glucose oxidase catalyzing as an example. Potassium iodide can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine in the presence of hydrogen peroxide to colorize the paper-based biosensor detection area, which was imaged by a scanner, and the color intensity was analyzed by the Adobe Photoshop. Under the optimal conditions, the color intensity shows a good linear relationship with hydrogen peroxide and glucose concentrations in the ranges of 0.1–5.0 mM and 0.5–6.0 mM, respectively. The detection limit of hydrogen peroxide is 0.03 mM and the limit of quantification of glucose is 0.5 mM. Besides, the method was employed in measuring glucose concentration in fruit samples, and the spiked recoveries are in the range of 95.4–106.1%. This method is cost-effective, environmentally friendly, and easy to be operated, which is expected to realize the point-of-care testing of more hydrogen-peroxide-related biomarkers.
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Otto, M., M. Snejdarkova et M. Rehak. « Hydrogen Peroxide/Oxygen Biosensor Based on Supported Phospholipid Bilayer ». Analytical Letters 25, no 4 (avril 1992) : 653–62. http://dx.doi.org/10.1080/00032719208020025.

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Chut, Shi Lin, Juan Li et Swee Ngin Tan∗. « A Mediated Turnip Tissue-Based Amperometric Hydrogen Peroxide Biosensor ». Analytical Letters 30, no 11 (août 1997) : 1993–98. http://dx.doi.org/10.1080/00032719708001715.

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Li, Juan, Swee Ngin Tan et Hailin Ge. « Silica sol-gel immobilized amperometric biosensor for hydrogen peroxide ». Analytica Chimica Acta 335, no 1-2 (décembre 1996) : 137–45. http://dx.doi.org/10.1016/s0003-2670(96)00337-6.

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Camacho, Conrado, Belkis Chico, Roberto Cao, Juan C. Matías, Javier Hernández, Ilaria Palchetti, Benjamin K. Simpson, Marco Mascini et Reynaldo Villalonga. « Novel enzyme biosensor for hydrogen peroxide via supramolecular associations ». Biosensors and Bioelectronics 24, no 7 (mars 2009) : 2028–33. http://dx.doi.org/10.1016/j.bios.2008.10.008.

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Li, Baoxin, Zhujun Zhang et Yan Jin. « Chemiluminescence flow biosensor for hydrogen peroxide with immobilized reagents ». Sensors and Actuators B : Chemical 72, no 2 (janvier 2001) : 115–19. http://dx.doi.org/10.1016/s0925-4005(00)00623-7.

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Liu, Jinping, Yuanyuan Li, Xintang Huang et Zhihong Zhu. « Tin Oxide Nanorod Array-Based Electrochemical Hydrogen Peroxide Biosensor ». Nanoscale Research Letters 5, no 7 (11 mai 2010) : 1177–81. http://dx.doi.org/10.1007/s11671-010-9622-1.

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Alim, Samiul, Rajan Jose, Mashitah M. Yusoff et A. K. M. Kafi. « An Amperometric Biosensor Based on a Multi-Nanoporous SnO2/HRP/Chitosan for Detection of Hydrogen Peroxide in Human Urine Samples ». Sensor Letters 17, no 10 (1 octobre 2019) : 769–76. http://dx.doi.org/10.1166/sl.2019.4145.

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A new amperometric biosensor fabricated from a multi-nanoporous SnO2 and HRP was trialled. The multiporous SnO2 nanofiber was fabricated in the research lab. Horseradish peroxidase was co-immobilized on a glassy carbon electrode with the multiporous SnO2 nanofiber and chitosan. The FESEM and EDX analysis were used to study the nanostructure and composition details of the nanofiber. Electrochemical results established that the immobilized HRP exhibited direct electrochemical behavior toward hydrogen peroxide (H2O2). The effects of pH, and applied potentials on the biosensor were also investigated. The limit of detection which was found to be 5 × 10–7 M with the linear range being between 5 × 10–6 M to 1.2 × 10–4 M of H2O2. For real sample analysis, this biosensor showed that the H2O2 concentration in the human urine can be monitored conveniently, where the interference is noted to be negligible.
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Radhakrishnan, Sivaprakasam, et Sang Jae Kim. « An enzymatic biosensor for hydrogen peroxide based on one-pot preparation of CeO2-reduced graphene oxide nanocomposite ». RSC Advances 5, no 17 (2015) : 12937–43. http://dx.doi.org/10.1039/c4ra12841a.

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The study describes cerium oxide-reduced graphene oxide (CeO2-rGO) prepared by a facile one-pot hydrothermal approach and its assembly with horseradish peroxidase (HRP) for the detection of hydrogen peroxide (H2O2) at trace levels.
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Gong, Coucong, Jingyi Chen, Yuan Shen, Yonggui Song, Yonghai Song et Li Wang. « Microperoxidase-11/metal–organic framework/macroporous carbon for detecting hydrogen peroxide ». RSC Advances 6, no 83 (2016) : 79798–804. http://dx.doi.org/10.1039/c6ra16145f.

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Roman, R. L., E. M. Turmena, L. Nagi, L. L. Silva, S. C. Fernandes, J. Dal Magro, J. M. M. Mello et Márcio Antônio Fiori. « Horseradish Peroxidase Immobilized on Polyaniline onto Monocrystalline Silicon for Glyphosate Herbicide Detection ». Materials Science Forum 930 (septembre 2018) : 603–8. http://dx.doi.org/10.4028/www.scientific.net/msf.930.603.

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In this work a special electrode configuration with potential application in enzymatic biosensors for the detection of glyphosate was studied. The enzyme used was Horseradish Peroxidase (HRP), which was immobilized on a polyaniline film (PAni), electrodeposited on the surface of the n-type monocrystalline silicon electrode. PAni has the ability to bind to biomolecules and thereby potentiate their biocatalytic properties by favoring the transfer of electrons between enzyme and substrate. Monocrystalline silicon is one of the most used materials in electronic technology due to its semiconductor character. In this work, different parameters were investigated in the electrode preparation, including concentration of polyaniline in the electrodeposition solutions, as well as the electrodeposition times and potentials. The response of the electrode as biosensor was evaluated by the electric current density characteristic of hydroquinone oxidation in the presence of standardized glyphosate solutions. The peroxidase enzyme catalyzes the oxidation of hydroquinone to the o-quinone form in the presence of hydrogen peroxide. In turn, glyphosate inhibits the activity of HRP and causes a reduction of the electric current density in the biosensor electrode. The results obtained with glyphosate using the proposed method are in agreement with the literature and show that the n-Si/PAni/HRP biosensor maintains the catalytic activity and is of considerable interest due to the simple procedure in practical applications and a promising platform for the lack of environmental monitoring for these contaminants.
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Sun, Yi-Xin, Jian-Tao Zhang, Shi-Wen Huang et Sheng-Fu Wang. « Hydrogen peroxide biosensor based on the bioelectrocatalysis of horseradish peroxidase incorporated in a new hydrogel film ». Sensors and Actuators B : Chemical 124, no 2 (26 juin 2007) : 494–500. http://dx.doi.org/10.1016/j.snb.2007.01.012.

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Li, Jun, Ke-Min Wang, Xiao-hai Yang et Dan Xiao. « Sol–gel horseradish peroxidase biosensor for the chemiluminescent flow determination of hydrogen peroxide ». Analytical Communications 36, no 5 (1999) : 195–97. http://dx.doi.org/10.1039/a901946d.

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Liu, Hongying, Chunchuan Gu, Weiwei Xiong et Mingzhen Zhang. « A sensitive hydrogen peroxide biosensor using ultra-small CuInS2 nanocrystals as peroxidase mimics ». Sensors and Actuators B : Chemical 209 (mars 2015) : 670–76. http://dx.doi.org/10.1016/j.snb.2014.12.052.

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Kang, Xiao Bin, Guang Chang Pang, Xin Yi Liang, Meng Wang, Jing Liu et Wei Ming Zhu. « Study on a hydrogen peroxide biosensor based on horseradish peroxidase/GNPs-thionine/chitosan ». Electrochimica Acta 62 (février 2012) : 327–34. http://dx.doi.org/10.1016/j.electacta.2011.12.034.

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Luo, Liqiang, Limei Zhu, Yanhong Xu, Liuyi Shen, Xia Wang, Yaping Ding, Qiuxia Li et Dongmei Deng. « Hydrogen peroxide biosensor based on horseradish peroxidase immobilized on chitosan-wrapped NiFe2O4 nanoparticles ». Microchimica Acta 174, no 1-2 (8 avril 2011) : 55–61. http://dx.doi.org/10.1007/s00604-011-0591-6.

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Roman, R. L., J. P. Z. Gonçalves, S. C. Fernandes, Lucinao Luiz Silva, J. Dal Magro, J. M. M. Mello et Márcio Antônio Fiori. « Iron Nanoparticles Coated with Nanostructured Carbon : Synthesis and Application in Glucose Biosensors ». Materials Science Forum 899 (juillet 2017) : 216–20. http://dx.doi.org/10.4028/www.scientific.net/msf.899.216.

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Nanowires of carbon coated ferric oxide (Fe2O3/C) have been studied to be applied at an electrochemical glucose biosensor as a new type of carbon paste electrode (CPE) modifier. The Fe2O3/C was synthesized by adapting co-precipitation hydrothermal route. Firstly, amperometric sensor based on a modified CPE doped with Fe2O3 has been developed and compared with CPE doped with Fe2O3/C for hydrogen peroxide (H2O2) determination. The performance evaluations for the modified materials and sensors were studied in detail through cyclic voltammetry (CV) method. After, the electrochemical sensor performance was also evaluated for glucose oxidase (GOx) response biosensor due to considerable interest in the blood sugar control. The results demonstrate that the GOx retains its biocatalytic activity toward the oxidation of glucose and that the bioelectrode modified by the Fe2O3/C matrix has potential for use in biosensors and other bioelectronics devices.
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Ortiz Santos, Elvis, Laura Galicia Luis, Maria Luisa Lozano et Gabriela Valdés-Ramírez. « Design of an Electrochemical Biosensor for the Quantification of H2O2 for Application to Cardiovascular Diseases ». ECS Transactions 110, no 1 (13 février 2023) : 333–38. http://dx.doi.org/10.1149/11001.0333ecst.

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Electrochemical biosensors, which are fast and reliable analytical tools, have attracted particular attention in recent years for their ability to incorporate biomolecules into nanomaterial-designed electrodes. In this study, an electrochemical biosensor for the detection of hydrogen peroxide (H2O2) was developed using a multi-walled carbon nanotube (EPMWC) platform electrochemically modified with poly [Fe (III)-5-Aphen] and using an oxide-reductase enzyme as recognition agent. Amperometry was applied to obtain the calibration curves for H2O2 at the reduction process. The analytical parameters such as sensitivity, linear range, and low detection limits for H2O2 were obtained. A sensitivity of 26.51µA/mM, linear range from (0.4 to 4.5) H2O2 mM and a LOD of 0.761 µM. The advance of this measurement system is the first step in the development process of an electrochemical biosensor for cardiovascular diseases applications.
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Fusco, Giovanni, Paolo Bollella, Franco Mazzei, Gabriele Favero, Riccarda Antiochia et Cristina Tortolini. « Catalase-Based Modified Graphite Electrode for Hydrogen Peroxide Detection in Different Beverages ». Journal of Analytical Methods in Chemistry 2016 (2016) : 1–12. http://dx.doi.org/10.1155/2016/8174913.

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A catalase-based (NAF/MWCNTs) nanocomposite film modified glassy carbon electrode for hydrogen peroxide (H2O2) detection was developed. The developed biosensor was characterized in terms of its bioelectrochemical properties. Cyclic voltammetry (CV) technique was employed to study the redox features of the enzyme in the absence and in the presence of nanomaterials dispersed in Nafion® polymeric solution. The electron transfer coefficient, α, and the electron transfer rate constant, ks, were found to be 0.42 and 1.71 s−1, at pH 7.0, respectively. Subsequently, the same modification steps were applied to mesoporous graphite screen-printed electrodes. Also, these electrodes were characterized in terms of their main electrochemical and kinetic parameters. The biosensor performances improved considerably after modification with nanomaterials. Moreover, the association of Nafion with carbon nanotubes retained the biological activity of the redox protein. The enzyme electrode response was linear in the range 2.5–1150 μmol L−1, with LOD of 0.83 μmol L−1. From the experimental data, we can assess the possibility of using the modified biosensor as a useful tool for H2O2 determination in packaged beverages.
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