Articles de revues : « HindIII »

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Mu'in, M. A., et A. G. Murwanto. « GHR/HindIII Locus Polymorphisms in Intron-2 GHR Gene of Papua Local Chicken ». Jurnal Sain Peternakan Indonesia 16, n^o 4 (26 décembre 2021) : 315–21. http://dx.doi.org/10.31186/jspi.id.16.4.315-321.

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This study aimed to detect single nucleotide polymorphisms (SNPs) in intron-2 on growth hormone receptor (GHR) gene in Papua local chickens using the PCR-RFLP method to study its relationship with growth characteristics. Data on the bodyweight of 49 chickens aged 1, 2, 3, and 4 months (22 males, 27 females) and DNA samples were used for this study. The DNA fragment of size 718 bp in intron-2 of the GHR gene from the study chicken was successfully amplified using a pair of specific primers. The PCR-RFLP/HindIII analysis results found this locus's two genotypes (HindIII++ and HindIII--). HindIII+ and HindIII- alleles were 0.02 and 0.98, respectively.

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Samson, M. L., et M. Wegnez. « Bipartite structure of the 5S ribosomal gene family in a Drosophila melanogaster strain, and its evolutionary implications. » Genetics 118, n^o 4 (1 avril 1988) : 685–91. http://dx.doi.org/10.1093/genetics/118.4.685.

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Abstract Knowledge of multigenic family organization should provide insight into their mode of evolution. Accordingly, we characterized the 5S ribosomal gene family in the Drosophila melanogaster strain ry506. The 5S genes in this strain display a striking HindIII restriction difference compared to the "standard" D. melanogaster 5S genes. The sequence of three ry506 5S genes was determined. We show that the HindIII restriction site heterogeneity within the ry506 5S family most probably results from the same point mutation, suggesting that a single 5S variant was propagated into the 5S cluster of this strain. Furthermore, we demonstrate that the structural organization of the 5S genes in ry506 is a bipartite structure, i.e., that about 40% of the 5S genes constitute a HindIII+/HindIII- mixed cluster, while those remaining constitute an homogeneous HindIII- cluster. The events which might lead to such an heterogeneous pattern are discussed from an evolutionary point of view.

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O'Connor, A., C. Nishigori, D. Yarosh, L. Alas, J. Kibitel, L. Burley, P. Cox, C. Bucana, S. Ullrich et M. Kripke. « DNA double strand breaks in epidermal cells cause immune suppression in vivo and cytokine production in vitro. » Journal of Immunology 157, n^o 1 (1 juillet 1996) : 271–78. http://dx.doi.org/10.4049/jimmunol.157.1.271.

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Abstract UV irradiation of the skin causes immune suppression by a mechanism involving epidermal cytokines. To determine the role of epidermal DNA damage in immune suppression, we used HindIII restriction endonuclease encapsulated in liposomes to cause DNA strand breaks in epidermal cells in vivo and in vitro. Topical application of HindIII in liposomes to murine skin in vivo impaired the induction of contact hypersensitivity responses initiated either locally or at distant sites and impaired the function of APCs. Unlike UV-B radiation, however, treatment of mice with HindIII in liposomes before contact sensitization did not induce tolerance or transferable suppression. The liposome-encapsulated HindIII caused double strand breaks in DNA and induced IL-10 and TNF-alpha production when added to cells of a murine keratinocyte line in vitro. Topical application of liposomal HindIII also induced TNF-alpha in the epidermis of mice. Liposomes containing heat-inactivated HindIII or an endonuclease specific for pyrimidine dimers in DNA did not exhibit these effects. These results support the hypothesis that DNA damage is a trigger for the production of cytokines that modulate immune responses. They also suggest that immune suppression and suppressor cell induction are separate consequences of cutaneous injury that require different stimuli.

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Pukkila, P. J., et C. Skrzynia. « Frequent changes in the number of reiterated ribosomal RNA genes throughout the life cycle of the basidiomycete Coprinus cinereus. » Genetics 133, n^o 2 (1 février 1993) : 203–11. http://dx.doi.org/10.1093/genetics/133.2.203.

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Abstract We have examined the stability of the tandemly repeated genes that encode the ribosomal RNA in Coprinus cinereus. These genes are contained within two linked HindIII fragments in a 3.0-Mb chromosome. We monitored the size of these fragments in both mitotic and meiotic segregants using the contour-clamped homogeneous electric field (CHEF) method. No length changes were observed in the smaller HindIII fragment (100 kb; 10 repeats) among the DNAs prepared from 46 asexual spore derivatives (oidia) or 128 meiotic segregants (basidiospores from 32 tetrads). However, the larger HindIII fragment (1100 kb; 120 repeats) did exhibit variability. Substantial changes, involving up to 40% of the larger HindIII fragment were recorded in 7 of 46 oidial isolates (including 4 of 22 transformed derivatives). To learn if the changes were confined to the vegetative portion of the life cycle, we examined transmission of HindIII variants through three crosses. In the first two crosses (16 tetrads total), no changes were observed in the large HindIII fragment. However, in the third cross (16 tetrads), each tetrad showed at least one alteration. In half of the tetrads from the third cross, the altered patterns segregated 2:2, suggesting that the changes occurred after mating but prior to premeiotic DNA replication. We conclude that breakage and rejoining reactions within the rDNA are frequent and are not confined to any particular stage of the life cycle. It also appears that certain repeats are sheltered from these events. Finally, marked differences in rDNA stability were observed in the cross analyzed.

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Kawamura, Takashi, Tomoki Kobayashi et Nobuhisa Watanabe. « Analysis of the HindIII-catalyzed reaction by time-resolved crystallography ». Acta Crystallographica Section D Biological Crystallography 71, n^o 2 (23 janvier 2015) : 256–65. http://dx.doi.org/10.1107/s1399004714025188.

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In order to investigate the mechanism of the reaction catalyzed by HindIII, structures of HindIII–DNA complexes with varying durations of soaking time in cryoprotectant buffer containing manganese ions were determined by the freeze-trap method. In the crystal structures of the complexes obtained after soaking for a longer duration, two manganese ions, indicated by relatively higher electron density, are clearly observed at the two metal ion-binding sites in the active site of HindIII. The increase in the electron density of the two metal-ion peaks followed distinct pathways with increasing soaking times, suggesting variation in the binding rate constant for the two metal sites. DNA cleavage is observed when the second manganese ion appears, suggesting that HindIII uses the two-metal-ion mechanism, or alternatively that its reactivity is enhanced by the binding of the second metal ion. In addition, conformational change in a loop near the active site accompanies the catalytic reaction.

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Coupar, Barbara E. H., Pamela G. Oke et Marion E. Andrew. « Insertion sites for recombinant vaccinia virus construction : effects on expression of a foreign protein ». Microbiology 81, n^o 2 (1 février 2000) : 431–39. http://dx.doi.org/10.1099/0022-1317-81-2-431.

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The expression of antigens or other molecules from recombinant vaccinia viruses requires the insertion of coding sequence at specific sites in the viral genome. Here we investigate the influence of two different sites on the level of protein expressed during a viral infection. The level of immune response in mice to vaccinia virus-expressed murine interleukin 2 (IL-2) or IL-4 varied depending on whether the coding sequence was inserted into the vaccinia virus thymidine kinase (tk) gene or into the HindIII F fragment of the viral genome where herpes simplex virus (HSV) tk was used as a selectable marker. In each case the intensity of the response was greater when the relevant gene was expressed from the HindIII F insertion site. In order to quantify these differences a series of recombinant viruses expressing luciferase was constructed. Luciferase activity from coding sequence inserted into the HindIII F fragment was significantly higher than that from the tk gene insertion, provided HSV tk+ constructs were compared. Insertion of a marker gene (HSV tk) into the HindIII F site with disruption of the F7L open reading frame led to a reduced level of luciferase expressed from the tk insert, despite more than 45 kb of intervening sequence. In mice, luciferase expression was higher from the HindIII F inserted gene than from the tk insert in both lungs and ovaries.

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Bora, N. S., T. W. Post et J. P. Atkinson. « Membrane cofactor protein of the complement system. A HindIII restriction fragment length polymorphism that correlates with the expression polymorphism. » Journal of Immunology 146, n^o 8 (15 avril 1991) : 2821–25. http://dx.doi.org/10.4049/jimmunol.146.8.2821.

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Abstract An RFLP was found in the DNA of 25 unrelated persons, two families, and five cell lines that correlated with their membrane cofactor protein phenotype. If restricted with HindIII, DNA derived from upper band predominant protein (U) phenotypes had a band at 2 kb, whereas DNA of lower band predominant (L) phenotypes had a 4-kb band. The equal band protein phenotype, in which equal quantities of the two species are expressed, had bands at both 4 and 2 kb. The polymorphic HindIII site was localized to an intron within the membrane cofactor protein gene between exon 1 (codes for 5'UT/signal peptide) and exon 2 (codes for the first short consensus repeat). Using the polymerase chain reaction (PCR), sequences around this site were amplified and a single band of 260 bp was produced. In the U phenotype, the PCR product was restricted with HindIII into 200- and 60-bp fragments. In the L phenotype, there was no change in the size of 260 bp upon restriction with HindIII. For the equal band protein phenotype, the PCR product was partially cleaved. The 260-bp PCR product was subcloned and sequenced. DNA from the U phenotype demonstrated an intact HindIII site (AAGCTT), whereas in the DNA of the L phenotype, this site was altered because a "G" was substituted for a "C" (AAGGTT).

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Wu, Wen-Luan, Jiang-Ping Wang, Mei-Chen Tseng et Tzen-Yuh Chiang. « Cloning and genetic variability of a HindIII repetitive DNA in Acrossocheilus paradoxus (Cyprinidae) ». Genome 42, n^o 4 (1 août 1999) : 780–88. http://dx.doi.org/10.1139/g99-019.

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Thirty clones of a highly repetitive HindIII fragment of DNA from seven populations of Acrossocheilus paradoxus (Cyprinidae) were isolated and sequenced. The fragment represents a tandemly repeated sequence, with a monomeric unit of 270 bp, amounting to 0.08-0.10% of the fish genome. Higher units of this monomer appear as a ladder in Southern blots. The HindIII satellite DNA family is conserved in three genera of the Cyprinidae. Variation in nucleotide sequences of this repetitive fragment, which is A+T-rich, is distributed both within individuals and among populations. High overall nucleotide divergence (dij= 0.056 ± 0.001) was detected among clones of the HindIII satellite DNAs of Acrossocheilus paradoxus. Based on the molecular clock hypothesis, the maximum evolutionary rate was estimated to be 5.3 × 10-7 substitutions per site per year. Lineage sorting may have contributed to the genetic heterogeneity within individuals and populations. Cladistic analyses indicated a closer phylogeographic relationship between populations of the central and south regions in Taiwan.Key words: highly repetitive DNA, HindIII restriction, nucleotide sequence, genetic variability, phylogeography.

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Abdulqader, Aveen M. Raouf, Shwan Rachid, Ali Ibrahim Mohammed et Sarwar Noori Mahmood. « Application of Indirect Linkage Analysis for Carrier Detection of Hemophilia A in Kurdistan Region of Iraq : Usefulness of Intron 18 BclI T>A, Intron 19 HindIII C>T, and IVS7 nt27 G>A Markers ». Clinical and Applied Thrombosis/Hemostasis 25 (1 janvier 2019) : 107602961985454. http://dx.doi.org/10.1177/1076029619854545.

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Hemophilia A (HA) is the most common congenital X-linked coagulopathy caused by mutations in the factor VIII gene. One in 5000 to 10 000 male persons worldwide suffer from HA. It is the archetype of high-cost, low-volume disease. Therefore, identification of carriers is crucial to avoid the birth of affected males. Tracking of the defective X chromosome through indirect linkage analysis represents the most practical method for screening for carriers in developing countries. In this study, 227 individuals from 41 families with HA and 100 normal participants were recruited from the Kurdistan region of Iraq and evaluated for intron 18 BclI, intron 19 HindIII, and IVS7 nt 27 markers by polymerase chain reaction restriction fragment length polymorphism and direct sequencing. Among the studied women, 49%, 42%, and 14% were discovered to be heterozygous for BclI, HindIII, and IVS7 markers, respectively. Using BclI, HindIII, and IVS7 markers, 56%, 46%, and 17% of the families were informative, respectively. The combined informativity of these polymorphic sites reaches 66%. The current study illustrates the effectiveness of the BclI and HindIII markers for the diagnosis of HA carriers among the Iraqi Kurdish population.

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Tao, Fang, Justin Weinstock, Scott A. Venners, Jun Cheng, Yi-Hsiang Hsu, Yanfeng Zou, Faming Pan, Shanqun Jiang, Xiangdong Zha et Xiping Xu. « Associations of the ABCA1 and LPL Gene Polymorphisms With Lipid Levels in a Hyperlipidemic Population ». Clinical and Applied Thrombosis/Hemostasis 24, n^o 5 (11 septembre 2017) : 771–79. http://dx.doi.org/10.1177/1076029617725601.

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We conducted a cross-sectional study to investigate the effects of the adenosine triphosphate-binding cassette transporter 1 (ABCA1) I883M and lipoprotein lipase (LPL) HindIII polymorphisms on lipid levels in patients with hyperlipidemia. A total of 533 patients were enrolled. Serum lipid parameters were determined by an automatic biochemistry analyzer. Genotyping of the ABCA1 I883M and LPL HindIII was carried out using the polymerase chain reaction-restriction fragment length polymorphism technique. Multiple linear regression analysis was used to estimate the associations between serum lipid levels and the genetic polymorphisms. The frequency distribution of the ABCA1 I883M and LPL HindIII polymorphisms did not deviate from Hardy-Weinberg equilibrium. The major finding of our regression analysis showed that neither the ABCA1 I883M nor the LPL HindIII polymorphism was associated with baseline serum lipid levels in the total population. However, among patients with elevated alanine aminotransferase (ALT) levels (ALT ≥ 40 U/L), carriers of the M allele of the ABCA1 gene had lower levels of high-density lipoprotein cholesterol (HDL-C) and higher levels of low-density lipoprotein cholesterol (LDL-C) after adjusting for age, sex, smoking status, alcohol consumption, education level, occupation, and work intensity ( P < .05 for both). A test on interaction terms between the ABCA1 I833M polymorphism and ALT on HDL-C and LDL-C levels also remained significant ( P = .001 and P = .014, respectively). Our data suggest that there are significant interactive effects between ABCA1 I883M and ALT levels on HDL-C and LDL-C levels. However, the LPL HindIII polymorphism did not influence lipid levels.

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Larson, Ilona, Michael M. Hoffmann, Jose M. Ordovas, Ernst J. Schaefer, Winfried März et Jörg Kreuzer. « The Lipoprotein Lipase HindIII Polymorphism : Association with Total Cholesterol and LDL-Cholesterol, but not with HDL and Triglycerides in 342 Females ». Clinical Chemistry 45, n^o 7 (1 juillet 1999) : 963–68. http://dx.doi.org/10.1093/clinchem/45.7.963.

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Abstract Background: Lipoprotein lipase (LPL) is the rate-limiting enzyme in the hydrolysis of core triglycerides in chylomicrons and VLDL. Methods: We investigated the association between the HindIII polymorphism of the LPL gene and fasting glucose, lipid, and lipoprotein concentrations in 683 Caucasians. We first stabilized the study subjects, using an 8-day diet and exercise intervention program before obtaining blood samples. The use of this standardization period reduced the variance of all glucose and lipid concentrations. Results: In our study, the HindIII allele frequencies for females and males were 0.29 and 0.34 for H− and 0.71 and 0.66 for H+, respectively. We found in females, but not in males, a significant association between the HindIII genotype and total cholesterol (P = 0.007) and LDL-cholesterol (P = 0.018), with females homozygous for the rare H− allele having the lowest, heterozygotes (H−/+) having intermediate, and women homozygous for the common H+ allele having the highest of each of these lipid traits. With regard to triglycerides, HDL-cholesterol, and glucose, no significant effect of the HindIII genotype was noted in either gender. Conclusions: These results suggest that in a gender-specific manner, the rare LPLHindIII H− allele has a cholesterol-lowering and, therefore, potentially cardioprotective effect compared with the common H+ allele.

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Nieradko, J., et B. Podgórska. « Expression of genes 51, 27, 28 coding for proteins of the central part of bacteriophage T4 baseplate in the bacteriophage T7 promoter/RNA polymerase expression system. » Acta Biochimica Polonica 40, n^o 2 (30 juin 1993) : 273–78. http://dx.doi.org/10.18388/abp.1993_4829.

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A fragment of T4 DNA (XbaI-HindIII) comprising the genes 51, 27, 28, which encodes the central plug proteins was cloned into plasmid pT7-5 and p7-6 (T7 RNA polymerase expressing system). The examined genes were only overexpressed when the orientation of cloned DNA to promoter phi 10 was as follows: promoter phi 10 and genes 51, 27, 28. This was achieved when the fragment (XbaI-HindIII) was cloned into plasmid pT7-5. Gene 27 and 28 were overexpressed when the intact fragment (XbaI-HindIII) was used. The high rate of the synthesis of proteins 27 and/or 28 had a strong inhibitory effect on the level of synthesis of the product of gene 51. For the overexpression of gene 51 in this system a deletion derivate which was devoid of gene 28 and a larger fragment of gene 27 was prepared.

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Mendez, Julio C., Mark J. Espy, Thomas F. Smith, Jennie A. Wilson et Carlos V. Paya. « Evaluation of PCR Primers for Early Diagnosis of Cytomegalovirus Infection following Liver Transplantation ». Journal of Clinical Microbiology 36, n^o 2 (1998) : 526–30. http://dx.doi.org/10.1128/jcm.36.2.526-530.1998.

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The availability of microbiologic methods that detect early replication of cytomegalovirus (CMV) posttransplantation will enhance the process of initiating preemptive antiviral therapy prior to the appearance of CMV disease. Using PCR techniques we sought to determine which region of the CMV genome present in peripheral blood leukocytes (PBLs) or serum provides the highest sensitivity for the detection of CMV posttransplantation. Blood samples were prospectively collected weekly for at least 8 weeks from a cohort of 21 consecutive liver transplant recipients not receiving anti-CMV prophylaxis. Results of PCR assays were correlated with recovery of CMV in cell cultures and histopathological findings from biopsy specimens of infected organs to assess clinical symptomatic infection. Of 148 specimens, primer pairs directed to the HindIII-X fragment region of CMV detected target DNA with a 94% sensitivity, compared to an 87% sensitivity with primer pairs directed to EcoRI fragment D, 32% sensitivity with primer pairs directed to the immediate-early antigen 1 gene (IEA1 gene), and 20% sensitivity with primer pairs directed to the major immediate-early (MIE) gene. The performance characteristics in terms of the sensitivity of primers for amplifying CMV DNA associated with symptomatic infection ranged from 100% (HindIII-X) to 20% (MIE gene); however, specificity was inversely related (HindIII-X, 45%; MIE gene, 91%) to primers directed to these gene targets. When HindIII-X andEcoRI-D primer sets were used, CMV DNA from PBLs was a more sensitive target than CMV DNA from serum for the early detection of symptomatic CMV infection (17 versus 12 days). Importantly, CMV DNA was not detected in five patients with no evidence of this viral infection. In conclusion, primers directed to the HindIII-X fragment region were the most optimal for the early detection of CMV DNA in PBLs and sera from symptomatic liver transplant recipients.

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W.Baron, Beverly, Christine Billstrand, Luisa Madronero et Timothy W. McKeithan. « HindIII polymorphism in the BCL6 gene ». Human Molecular Genetics 2, n^o 9 (1993) : 1513. http://dx.doi.org/10.1093/hmg/2.9.1513.

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Franck, Jens P. C., et Jonathan M. Wright. « Conservation of a satellite DNA sequence (SATB) in the tilapiine and haplochromine genome (Pisces : Cichlidae) ». Genome 36, n^o 1 (1 février 1993) : 187–94. http://dx.doi.org/10.1139/g93-025.

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We have cloned and sequenced a 1900-bp EcoRI fragment (SATB) from the tilapiine fish Oreochromis niloticus. The SATB sequence is highly reiterated in the tilapiine genome and organized in long tandem arrays. A 760-bp HindIII fragment, an internal component of SATB, has also been cloned and sequenced from the related tilapiine species Oreochromis hornorum. Hybridization of the radiolabeled 760-bp HindIII repeat detected the presence of the SATB repeat in the genomes of several tilapiine species as well as the haplochromine species Haplochromis (Protomelas) similis. The 760-bp HindIII fragment did not hybridize to genomic DNA of Etroplus maculatus (an Asian cichlid) or to that of Cichlasoma meeki (a South American cichlid). The SATB repeat sequence is 56% AT and constitutes 0.2–5% of the tilapiine genome depending on the species examined. Four imperfect 21-bp direct repeat sequences are present within the cloned 1900-bp EcoRI repeat. Alignment of the four direct repeats from the O. niloticus cloned 1900-bp DNA and the two homologous direct repeats from the O. hornorum 760-bp HindIII repeat revealed a core motif of 11 bp that exhibits 100% sequence identity between all of the direct repeats. The conservation of this motif in the SATB repeat suggests that this sequence may be under selective constraint.Key words: satellite DNA, Cichlidae, direct repeats.

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Zhu, X., K. P. Pruess et T. O. Powers. « Mitochondrial DNA polymorphism in a black fly, Simulium vittatum (Diptera : Simuliidae) ». Canadian Journal of Zoology 76, n^o 3 (1 mars 1998) : 440–47. http://dx.doi.org/10.1139/z97-203.

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Mitochondrial DNA (mtDNA) was extracted from pooled field-collected samples representing six species of black flies (Cnephia dacotensis, Simulium bivittaum, S. johansenni, S. luggeri, S. piperi, S. vittatum) and compared by restriction fragment length polymorphism (RFLP) analysis. Morphospecies were molecularly distinct, with few shared restriction fragments. Eleven populations of S. vittatum were found that appeared to be homogeneous for a single mitochondrial haplotype. Ten other populations of S. vittatum showed extensive mitochondrial heterogeneity. In part, these samples contained mixtures of two cytologically recognized siblings: IIIL-1 and IS-7. About 70% of the mitochondrial genome of a population pure for sibling IIIL-1 was cloned as five HindIII fragments, which were used as hybridization probes to examine individual black flies. Thirteen mtDNA haplotypes involving permutations of 10 HindIII restriction sites were identified in individual black flies examined from 26 populations. DNA from 168 larvae cut with both EcoR1 and HindIII revealed five additional haplotypes. One HindIII haplotype was present in 84% of 390 larvae examined and predominated in every population examined from New York to California and in both the IIIL-1 and IS-7 siblings. Nebraska populations had individuals with nearly all known haplotypes. The most common haplotype was usually the only form present in warm, silty streams with organic enrichment. Rarer haplotypes were found in cool, spring-fed streams but without clear geographic or phylogenetic components.

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Borthakur, Dulal, et Xuefeng Gao. « A 150-megadalton plasmid in Rhizobium etli strain TAL182 contains genes for nodulation competitiveness on Phaseolus vulgaris L. » Canadian Journal of Microbiology 42, n^o 9 (1 septembre 1996) : 903–10. http://dx.doi.org/10.1139/m96-116.

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Rhizobium etli TAL182, a competitive strain for the nodulation of Phaseolus beans, occupied more than 99% of the nodules when co-inoculated in various proportions with Rhizobium TAL1145 or Rhizobium tropici CIAT899. Two overlapping cosmid clones, pUHR68 and pUHR69, containing genes for nodulation competitiveness from TAL182, were isolated by functional complementation of strain TAL1145. Using one of these cosmid clones, we constructed two Tn5-insertion mutants of TAL182 defective in nodulation competitiveness. The Tn5 insertions in both mutants were localized in identical positions within a 4.6-kb HindIII fragment. One mutant, RUH120, was complemented for nodulation competitiveness by this HindIII fragment. The cloned DNA in pUHR68 is a part of a plasmid, 150 MDa in size, in TAL182 and does not show homology with TAL1145 genomic DNA. The 4.6-kb HindIII fragment contains a gene(s) required for nodulation competitiveness on beans, which is present only in some R. etli strains and absent in other Rhizobium spp.Key words: nitrogen fixation, competition, nodule, common bean.

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Mruk, Iwona, et Tadeusz Kaczorowski. « Genetic Organization and Molecular Analysis of the EcoVIII Restriction-Modification System of Escherichia coli E1585-68 and Its Comparison with Isospecific Homologs ». Applied and Environmental Microbiology 69, n^o 5 (mai 2003) : 2638–50. http://dx.doi.org/10.1128/aem.69.5.2638-2650.2003.

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ABSTRACT The EcoVIII restriction-modification (R-M) system is carried by the Escherichia coli E1585-68 natural plasmid pEC156 (4,312 bp). The two genes were cloned and characterized. The G+C content of the EcoVIII R-M system is 36.1%, which is significantly lower than the average G+C content of either plasmid pEC156 (43.6%) or E. coli genomic DNA (50.8%). The difference suggests that there is a possibility that the EcoVIII R-M system was recently acquired by the genome. The 921-bp EcoVIII endonuclease (R · EcoVIII) gene (ecoVIIIR) encodes a 307-amino-acid protein with an M r of 35,554. The convergently oriented EcoVIII methyltransferase (M · EcoVIII) gene (ecoVIIIM) consists of 912 bp that code for a 304-amino-acid protein with an M r of 33,930. The exact positions of the start codon AUG were determined by protein microsequencing. Both enzymes recognize the specific palindromic sequence 5′-AAGCTT-3′. Preparations of EcoVIII R-M enzymes purified to homogeneity were characterized. R · EcoVIII acts as a dimer and cleaves a specific sequence between two adenine residues, leaving 4-nucleotide 5′ protruding ends. M · EcoVIII functions as a monomer and modifies the first adenine residue at the 5′ end of the specific sequence to N 6-methyladenine. These enzymes are thus functionally identical to the corresponding enzymes of the HindIII (Haemophilus influenzae Rd) and LlaCI (Lactococcus lactis subsp. cremoris W15) R-M systems. This finding is reflected by the levels of homology of M · EcoVIII with M · HindIII and M · LlaCI at the amino acid sequence level (50 and 62%, respectively) and by the presence of nine sequence motifs conserved among m6 N-adenine β-class methyltransferases. The deduced amino acid sequence of R · EcoVIII shows weak homology with its two isoschizomers, R · HindIII (26%) and R · LlaCI (17%). A catalytic sequence motif characteristic of restriction endonucleases was found in the primary structure of R · EcoVIII (D108X12DXK123), as well as in the primary structures of R · LlaCI and R · HindIII. Polyclonal antibodies raised against R · EcoVIII did not react with R · HindIII, while anti-M · EcoVIII antibodies cross-reacted with M · LlaCI but not with M · HindIII. R · EcoVIII requires Mg(II) ions for phosphodiester bond cleavage. We found that the same ions are strong inhibitors of the M · EcoVIII enzyme. The biological implications of this finding are discussed.

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Dion, Michel, et Claude Hamelin. « Cartographie physique de l'ADN du cytomégalovirus humain souche AD169 ». Canadian Journal of Microbiology 36, n^o 5 (1 mai 1990) : 341–47. http://dx.doi.org/10.1139/m90-059.

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The whole human cytomegalovirus strain AD169 genome was cloned into plasmid pAT153 in the form of 25 HindIII fragments. Double and triple digestions of the recombinant plasmids with restriction endonucleases BamHI, BglII, ClaI, DraI, EcoRI, EcoRV, HindIII, HpaI, KpnI, PaeR7, PstI, SphI and XbaI yielded a detailed restriction map of human cytomegalovirus DNA. Knowing the exact position of numerous restriction sites in the viral DNA molecule, we have been able to examine very closely the heterologous region between the long and the short segments of the human cytomegalovirus genome. Key words: DNA, physical map, cytomegalovirus, restriction endonucleases, HCMV.

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Palacio Rojas, Marcos, Carem Prieto, Valmore Bermúdez, Carlos Garicano, Trina Núñez Nava, María Sofía Martínez, Juan Salazar et al. « Dyslipidemia : Genetics, lipoprotein lipase and HindIII polymorphism ». F1000Research 6 (30 novembre 2017) : 2073. http://dx.doi.org/10.12688/f1000research.12938.1.

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The direct link between lipid metabolism alterations and the increase of cardiovascular risk are well documented. Dyslipidemias, including isolated high LDL-c or mixed dyslipidemia, such as those seen in diabetes (hypertriglyceridemia, high LDL-c or low HDL-c), correlate with a significant risk of cardiovascular and cerebrovascular disease worldwide. This review analyzes the current knowledge concerning the genetic basis of lipid metabolism alterations, emphasizing lipoprotein lipase gene mutations and the HindIII polymorphism, which are associated with decreased levels of triglycerides and LDL-c, as well as higher levels of HDL-c. These patterns would be associated with decreased global morbidity and mortality, providing protection against cardiovascular and cerebrovascular diseases.

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Palacio Rojas, Marcos, Carem Prieto, Valmore Bermúdez, Carlos Garicano, Trina Núñez Nava, María Sofía Martínez, Juan Salazar et al. « Dyslipidemia : Genetics, lipoprotein lipase and HindIII polymorphism ». F1000Research 6 (1 octobre 2018) : 2073. http://dx.doi.org/10.12688/f1000research.12938.2.

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The direct link between lipid metabolism alterations and the increase of cardiovascular risk are well documented. Dyslipidemias, including isolated high LDL-c or mixed dyslipidemia, such as those seen in diabetes (hypertriglyceridemia, high LDL-c or low HDL-c), correlate with a significant risk of cardiovascular and cerebrovascular disease worldwide. This review analyzes the current knowledge concerning the genetic basis of lipid metabolism alterations, emphasizing lipoprotein lipase gene mutations and the HindIII polymorphism, which are associated with decreased levels of triglycerides and LDL-c, as well as higher levels of HDL-c. These patterns would be associated with decreased global morbidity and mortality, providing protection against cardiovascular and cerebrovascular diseases.

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Vielh, E., G. Uzé, G. Lutfalla, M. T. Bandu et K. E. Mogensen. « HindIII RFLP at the human IFNAR locus ». Nucleic Acids Research 18, n^o 18 (1990) : 5580. http://dx.doi.org/10.1093/nar/18.18.5580.

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Hoefsloot, L. H., M. Hoogeveek-Westerveld, H. Sakuraba, Y. Suzuki, B. A. Oostra et A. J. J. Reuser. « HindIII/EcoRI polymorphism in the GAA gene ». Nucleic Acids Research 18, n^o 19 (1990) : 5921. http://dx.doi.org/10.1093/nar/18.19.5921.

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Fourney, R. M., K. D. Dietrich, R. A. Aubin et M. C. Paterson. « HindIII polymorphism in the humanc-sisproto-oncogene ». Nucleic Acids Research 16, n^o 16 (1988) : 8197. http://dx.doi.org/10.1093/nar/16.16.8197.

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SUTARNO, SUTARNO, SYARIFA ZAHRAH, OKID PARAMA ASTIRIN, ELISA HERAWATI et AHMAD DWI SETYAWAN. « Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique ». Biodiversitas Journal of Biological Diversity 20, n^o 3 (2 mars 2019) : 783–88. http://dx.doi.org/10.13057/biodiv/d200324.

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Abstract. Sutarno, Zahrah S, Astirin OP, Herawati E, Setyawan AD. 2019. Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique. Biodiversitas 20: 783-788. Genetic diversity is the basis of livestock breeding because it can be used as an initial improvement in livestock quality through artificial selection. This study aims to determine polymorphism in ND-5 fragment of mitochondrial DNA in Ongole Grade, Aceh, and Sumbawa cattle and their genetic diversity. The total DNA from the blood of the local cattle was extracted using the Wizard genomic DNA purification system from Promega and amplified using the PCR technique. The PCR product was then digested with HindIII enzyme using the RFLP technique to detect polymorphism. The genetic diversity of the Ongole Grade, Aceh, and Sumbawa cattle was analyzed using the formula from Nei and its genetic relationship was evaluated with the 2.02i NTSYSpc version program. Our findings showed there were polymorphisms in the ND-5 fragment of mitochondrial DNA. Digestion with HindIII restriction enzyme produces two types of haplotypes. Haplotype B is a 453 bp-sized DNA fragment that is not truncated by the HindIII enzyme, and haplotype A is a DNA fragment cut by HindIII enzyme into two with fragments of 336 bp and 117 bp. Polymorphism was found in Ongole Grade cattle, but not in Sumbawa and Aceh cattle. Haplotypes diversity in ND-5 fragments of mitochondrial DNA of Ongole Grade was 0.6250 while Sumbawa and Aceh cattle displayed no diversity of haplotypes. The genetic relationship shows that Sumbawa cattle belonged to the same cluster with Ongole Grade but separated from Aceh cattle.

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Luo, Meizhong, Yi-Hong Wang, David Frisch, Tarek Joobeur, Rod A. Wing et Ralph A. Dean. « Melon bacterial artificial chromosome (BAC) library construction using improved methods and identification of clones linked to the locus conferring resistance to melon Fusarium wilt (Fom-2) ». Genome 44, n^o 2 (1 avril 2001) : 154–62. http://dx.doi.org/10.1139/g00-117.

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Utilizing improved methods, two bacterial artificial chromosome (BAC) libraries were constructed for the multidisease-resistant line of melon MR-1. The HindIII library consists of 177 microtiter plates in a 384-well format, while the EcoRI library consists of 222 microtiter plates. Approximately 95.6% of the HindIII library clones contain nuclear DNA inserts with an average size of 118 kb, providing a coverage of 15.4 genome equivalents. Similarly, 96% of the EcoRI library clones contain nuclear DNA inserts with an average size of 114 kb, providing a coverage of 18.7 genome equivalents. Both libraries were evaluated for contamination with high-copy vector, empty pIndigoBac536 vector, and organellar DNA sequences. High-density filters were screened with two genetic markers FM and AM that co-segregate with Fom-2, a gene conferring resistance to races 0 and 1 of Fusarium wilt. Fourteen and 18 candidate BAC clones were identified for the FM and AM probes, respectively, from the HindIII library, while 34 were identified for the AM probe from filters A, B, and C of the EcoRI library.Key words: bacterial artificial chromosome (BAC) library, Fusarium wilt, melon, pCUGIBAC1, resistant gene.

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Syed Tasleem Raza, Nuzhat Husain, Anant Narayan Bhatt et Avneesh. « HindIII Polymorphism in Carrier Detection of Hemophilia A in India : A New Primer Design ». Clinical and Applied Thrombosis/Hemostasis 13, n^o 4 (octobre 2007) : 432–34. http://dx.doi.org/10.1177/1076029607303527.

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HindIII polymorphism studies were done in 67 north Indian families with hemophilia A using a new primer sequence and comparing results with the standard protocol. Amplified segment was 608 bp in size. Genetically positive hemizygous males (+) and homozygous females (+/+) showed 427, 100, and 81 size fragments, whereas heterozygous females (+/−) had 427, 181, 100, and 81-bp segments. Genotypically negative hemizygous (−) males and homozygous females (−/−) show that 427-bp and 181-bp fragments dimer formation was minimal and results matched with standard protocol in 20 cases where both polymerase chain reactions were run. The frequency of positive allele for HindIII polymorphism in 67 families was 0.38 and heterozygosity in terms of informativity was 0.46.

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Chumachenko, Yaroslav D., Viktoriia Yu Harbuzova et Alexander V. Ataman. « Association Study between BGLAP Gene HindIII Polymorphism and Type 2 Diabetes Mellitus Development in Ukrainian Population ». Journal of Diabetes Research 2019 (27 novembre 2019) : 1–7. http://dx.doi.org/10.1155/2019/9302636.

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Type 2 diabetes mellitus (T2DM) belongs to the diseases with hereditary predisposition, so both environmental and genetic factors contribute to its development. Recent studies have demonstrated that the skeleton realizes systemic regulation of energy metabolism through the secretion of osteocalcin (OCN). Thus, the association analysis between HindIII single nucleotide polymorphism of OCN gene (BGLAP) promoter region and T2DM development in Ukrainian population was carried out. 153 individuals diagnosed with T2DM and 311 control individuals were enrolled in the study. The genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The lack of association between BGLAP HindIII single nucleotide polymorphism (SNP) and T2DM development among Ukrainians was found. Further studies with extended groups of comparison are needed to confirm the obtained results.

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Kenney, Richard T., et Thomas L. Leto. « A HindIII polymorphism in the human NCF2 gene ». Nucleic Acids Research 18, n^o 23 (1990) : 7193. http://dx.doi.org/10.1093/nar/18.23.7193-a.

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Habib, Saman, et Seyed E. Hasnain. « Differential Activity of Two Non-hrOrigins during Replication of the Baculovirus Autographa californica Nuclear Polyhedrosis Virus Genome ». Journal of Virology 74, n^o 11 (1 juin 2000) : 5182–89. http://dx.doi.org/10.1128/jvi.74.11.5182-5189.2000.

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ABSTRACT The identification of potential baculovirus origins of replication (ori) has involved the generation and characterization of defective interfering particles that contain major genomic deletions yet retain their capability to replicate by testing the replication ability of transiently transfected plasmids carrying viral sequences in infected cells. So far, there has not been any evidence to demonstrate the actual utilization of these putative origins in Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV) replication. By using the method of origin mapping by competitive PCR, we have obtained quantitative data for theori activity of the HindIII-K region and theie-1 promoter sequence in AcMNPV. We also provide evidence for differential activity of the two oriin the context of the viral genome through the replication phase of viral infection. Comparison of the number of molecules representing theHindIII-K and ie-1 origins vis-à-vis the non-ori polH region in a size-selected nascent DNA preparation revealed that the HindIII-K ori is utilized ∼14 times more efficiently than the ie-1 region during the late phase of infection. HindIII-K also remains the more active ori through the early and middle replication phases. Our results provide in vivo evidence in support of the view that AcMNPV replication involves multipleori that are activated with vastly different efficiencies during the viral infection cycle.

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Nishigori, Chikako, Daniel Yarosh, Adrienne O’Connor, Vijay K. Shreedhar, Stephen E. Ullrich, Patricia Cox et Margaret L. Kripke. « HindIII Liposomes Suppress Delayed-Type Hypersensitivity Responses In Vivo and Induce Epidermal IL-10 In Vitro ». Journal of Immunology 161, n^o 6 (15 septembre 1998) : 2684–91. http://dx.doi.org/10.4049/jimmunol.161.6.2684.

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Abstract Considerable evidence suggests that ultraviolet-B (UV-B) radiation suppresses certain immune responses through the induction of cyclobutane pyrimidine dimers in DNA. To determine whether induction of other forms of DNA damage in the skin mimicked the immunosuppressive effects of UV-B radiation, we produced double-strand breaks in the DNA of epidermal cells with HindIII restriction endonuclease encapsulated in liposomes. Application of these liposomes, but not liposomes containing inactive HindIII or an irrelevant endonuclease, to the skin of C3H mice suppressed the induction of delayed-type hypersensitivity responses to Candida albicans and alloantigen and induced IL-10 production in the epidermis. Treatment of the Pam212 murine keratinocyte cell line with these liposomes in vitro induced immunosuppressive activity and IL-10 in culture supernatants. Unlike UV-B irradiation, however, HindIII in liposomes failed to induce suppressor T cell activity in vivo or in vitro. We conclude that double-strand breaks in DNA of epidermal cells can induce immunosuppression and up-regulate the production of immunomodulatory cytokines; however, either DNA damage alone does not account for all the immunosuppressive properties of UV-B irradiation, or cyclobutane pyrimidine dimers differ qualitatively from double-strand breaks in their biologic consequences. These studies raise the possibility that drugs causing DNA damage may induce cytokine dysregulation and immune suppression in addition to cytotoxicity.

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Iso, Hiroyasu, Aaron R. Folsom, John C. Winkelmann, Kazuko Koike, Shoji Harada, Beryl Greenberg, Shinichi Sato, Takashi Shimamoto, Minoru lida et Yoshio Komachi. « Polymorphisms of the Beta Fibrinogen Gene and Plasma Fibrinogen Concentration in Caucasian and Japanese Population Samples ». Thrombosis and Haemostasis 73, n^o 01 (1995) : 106–11. http://dx.doi.org/10.1055/s-0038-1653733.

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SummaryWe reported previously that plasma fibrinogen was significantly higher in U.S. Caucasians than in Japanese, which may contribute to the higher mortality rate of coronary heart disease in the United States than in Japan. To examine the contribution of genetic variations to the race difference in plasma fibrinogen levels, restriction fragment length polymorphisms (RFLPs) of the beta fibrinogen gene were examined in 293 nonsmoking Caucasians and Japanese men and women aged 47-69 years. Three RFLPs were detected by digestion of genomic DNA using the BclI restriction enzyme, polymerase chain reaction (PCR) products using HaeIII and HindIII. The alleles B2 (4.2 kb, BclI digestion), H2 (957 b, HaeIII) and Hd2 (465 b, HindIII) were associated with higher fibrinogen concentrations in previous studies. Because of a strong linkage disequilibrium between HaeIII and HindIII polymorphisms, the data of HindIII was presented. The frequency of the B2 allele was 22% (95% Cl: 17-27%) for Caucasians and 13% (10-17%) for Japanese (the difference: p <0.01). The respective frequency of the Hd2 allele was 26% (21-31%) and 12% (8-16%) (p <0.001). After controlling for age, body mass index, alcohol intake, triglycerides, fish intake, and for women, menopausal status and hormone replacement therapy, the adjusted mean fibrinogen level among Caucasians was 289 mg/dl for genotype B1B1 and 301 mg/dl for genotype B1B2 or B2B2 combined (p = 0.18), and 285 mg/dl for Hd1Hd1 and 306 mg/dl for Hd1Hd2 or Hd2Hd2 combined (p = 0.03). The respective mean values among Japanese were 251 mg/dl for B1B1 and 257 mg/dl for B1B2 or B2B2 combined (p = 0.37), 248 mg/dl for Hd1Hd1 and 259 mg/dl for Hd1Hd2 or Hd2Hd2 combined (p = 0.16). These polymorphisms had a similar effect on plasma fibrinogen concentrations for both men and women. The results suggest that a genetic difference may partly explain the higher plasma fibrinogen in Caucasians than in Japanese.

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Wilson, J. G., W. W. Wong, E. E. Murphy, P. H. Schur et D. T. Fearon. « Deficiency of the C3b/C4b receptor (CR1) of erythrocytes in systemic lupus erythematosus : analysis of the stability of the defect and of a restriction fragment length polymorphism of the CR1 gene. » Journal of Immunology 138, n^o 8 (15 avril 1987) : 2706–10. http://dx.doi.org/10.4049/jimmunol.138.8.2706.

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Abstract The role of genetic factors in controlling CR1 quantitative expression on erythrocytes (E) of patients with systemic lupus erythematosus (SLE) was reexamined by determining the temporal stability of CR1 numbers and the frequency of a CR1 genomic restriction fragment length polymorphism (RFLP). The mean number of binding sites/(E) for Yz-1 monoclonal anti-CR1 correlated with the number of sites for polyclonal anti-CR1 that had been determined 2 to 4 yr previously in 18 normal persons (p less than 0.001), 18 patients (p less than 0.001), and 28 relatives (p less than 0.001), indicating that CR1 sites/E was a stable characteristic in all three groups. The mean number of Yz-1 sites/E was 281 +/- 34 (+/- SEM) in 28 probands with SLE and 457 +/- 21 in 93 relatives, both determinations being less than that for 100 normal persons, 553 +/- 21 (p less than 0.002). Thirty-six patients and 51 normal individuals were also assessed for the presence of the 7.4 kb and 6.9 kb HindIII CR1 allelic restriction fragments that correlate with high and low expression, respectively, of CR1 on E. The distribution of patients differed from normal (p less than 0.05), with a smaller proportion being homozygous for the 7.4 kb allele. In addition, the mean numbers of Yz-1 sites/E for patients and relatives who were homozygous (p less than 0.02) and heterozygous (p less than 0.05) for the 7.4 kb allele were significantly lower than those for normal persons matched for the HindIII RFLP, suggesting the existence of additional heritable factors that decrease CR1 expression. The stability over time of the CR1 deficiency among patients, the finding of decreased CR1 number among an expanded group of relatives, the altered frequency among patients of CR1 alleles defined by the HindIII RFLP, and the decreased expression of CR1 on E among patients and relatives compared with normal individuals having the same HindIII RFLP indicate a role for genetic factors in CR1 deficiency in SLE.

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Fjellstrom, R. G., et D. E. Parfitt. « Walnut (Juglans spp.) genetic diversity determined by restriction fragment length polymorphisms ». Genome 37, n^o 4 (1 août 1994) : 690–700. http://dx.doi.org/10.1139/g94-097.

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The genetic diversity of 13 Juglans species was characterized using nuclear RFLPs. Allelic frequencies among 41 Juglans populations were determined at 19 RFLP loci by hybridizing single locus probes to walnut DNAs digested with the restriction endonuclease EcoRI or HindIII. A 10-fold difference in species heterozygosity levels was seen among species in different sections of the genus. Differentiation among conspecific populations varied over threefold between species. Genetic differentiation among conspecific east Asian populations was larger than that seen among east Asian species, while the opposite trend was seen for Western Hemisphere species. Taxonomic affinities were also indicated by these results, suggesting that J. cinerea should be included as part of section Cardiocaryon rather than as a unique section, Trachycaryon. Juglans hindsii is classified as a distinct species and not a subspecies of J. californica. Strategies for germplasm preservation and species requiring marked collection efforts are given.Key words: Juglans, RFLP, genetic diversity, walnut.

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Subramanian, T., M. Kuppuswamy et G. Chinnadurai. « An adenovirus 2-coded tumor antigen located on the endoplasmic reticulum and nuclear envelope is required for growth of transformed cells in Ca2+-deficient media ». Molecular and Cellular Biology 5, n^o 11 (novembre 1985) : 3297–300. http://dx.doi.org/10.1128/mcb.5.11.3297-3300.1985.

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Rat embryo cell lines containing the adenovirus 2 E1a region together with normal or mutant forms of the N-terminal half of the E1b region (HindIII G fragment) were generated by using a dominant selection marker, neo. Biochemically transformed cells containing a nonmutated HindIII G fragment proliferated more rapidly in Ca2+-deficient media, whereas cells containing a specific deletion within the E1b-encoded, 175-amino-acid (175R) (19-kilodalton) T-antigen gene and nontransformed cells grew at a slower rate. Furthermore, transformed cells that did not express the 175R T antigen and untransformed cells could not replicate their DNA efficiently in low-Ca2+ medium. Our results suggest that Ca2+ ions may provide an important stimulus for cell proliferation in adenovirus-transformed cells through a mechanism that involves the functions of the 175R T antigen.

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Subramanian, T., M. Kuppuswamy et G. Chinnadurai. « An adenovirus 2-coded tumor antigen located on the endoplasmic reticulum and nuclear envelope is required for growth of transformed cells in Ca2+-deficient media. » Molecular and Cellular Biology 5, n^o 11 (novembre 1985) : 3297–300. http://dx.doi.org/10.1128/mcb.5.11.3297.

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Rat embryo cell lines containing the adenovirus 2 E1a region together with normal or mutant forms of the N-terminal half of the E1b region (HindIII G fragment) were generated by using a dominant selection marker, neo. Biochemically transformed cells containing a nonmutated HindIII G fragment proliferated more rapidly in Ca2+-deficient media, whereas cells containing a specific deletion within the E1b-encoded, 175-amino-acid (175R) (19-kilodalton) T-antigen gene and nontransformed cells grew at a slower rate. Furthermore, transformed cells that did not express the 175R T antigen and untransformed cells could not replicate their DNA efficiently in low-Ca2+ medium. Our results suggest that Ca2+ ions may provide an important stimulus for cell proliferation in adenovirus-transformed cells through a mechanism that involves the functions of the 175R T antigen.

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Waeyenberge, Lieven, Alexander Ryss, Maurice Moens, Jorge Pinochet et Thierry Vrain. « Molecular characterisation of 18 Pratylenchus species using rDNA Restriction Fragment Length Polymorphism ». Nematology 2, n^o 2 (2000) : 135–42. http://dx.doi.org/10.1163/156854100509024.

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AbstractThe RFLP technique was used to establish a reliable diagnostic method for 18 Pratylenchus species: Pratylenchus agilis, P. bolivianus, P. brachyurus, P. coffeae, P. crenatus, P. fallax, P. goodeyi, P. loosi, P. mediterraneus, P. neglectus, P. penetrans, P. pratensis, P. pseudocoffeae, P. scribneri, P. subranjani, P. thornei, P. vulnus and P. zeae. The polymerase chain reaction (PCR) amplified the ITS regions from all species and populations examined and revealed large differences in length, ranging in size from approximately 900 to 1250 bp. The rDNA fragments were digested with five restriction enzymes (CfoI, DdeI, HindIII, HpaII, and PstI). All Pratylenchus species can be differentiated from each other by a combination of at least two enzymes. CfoI differentiated all nematode species with the exception of P. fallax, P. penetrans and P. pseudocoffeae. P. fallax was further separated by a DdeI restriction, and P. pseudocoffeae by a PstI digestion. Intraspecific RFLP were observed. Upon CfoI, DdeI, HindIII, or HpaII digestion, it was possible to separate the three P. coffeae populations studied from each other. La technique RFLP a été utilisée pour créer une méthode fiable de diagnostic pour 18 espèces de Pratylenchus: Pratylenchus agilis, P. bolivianus, P. brachyurus, P. coffeae, P. crenatus, P. fallax, P. goodeyi, P. loosi, P. mediterraneus, P. neglectus, P. penetrans, P. pratensis, P. pseudocoffeae, P. scribneri, P. subranjani, P. thornei, P. vulnus et P. zeae. La réaction de polymérisation en chaîne (PCR) a amplifié les régions de l’ITS pour toutes les espèces et populations étudiées et a mis en évidence de grandes différences dans la taille des gammes de longueur, de 900 à 1250 bp approximativement. Les fragments de rDNA ont été digérés à l’aide de cinq enzymes de restriction (CfoI, DdeI, HindIII, HpaII, and PstI). Toutes les espèces de Pratylenchus ont pu être différenciées les unes des autres par une combinaison d’au moins deux enzymes. CfoI a différencié toutes les espèces à l’exception de P. fallax, P. penetrans et P. pseudocoffeae. P. fallax a été ultérieurement séparé par une restriction DdeI, et P. pseudocoffeae par une digestion PstI. Des RFLP intraspécifiques ont été observés. Par les digestions CfoI, DdeI, HindIII, ou HpaII, il s’est révélé possible de séparer les unes des autres les trois populations étudiées de P. coffeae.

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Shiramizu, B., F. Barriga, J. Neequaye, A. Jafri, R. Dalla-Favera, A. Neri, M. Guttierez, P. Levine et I. Magrath. « Patterns of chromosomal breakpoint locations in Burkitt's lymphoma : relevance to geography and Epstein-Barr virus association ». Blood 77, n^o 7 (1 avril 1991) : 1516–26. http://dx.doi.org/10.1182/blood.v77.7.1516.1516.

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Abstract We have examined, by Southern blotting, the patterns of chromosomal breakpoint locations in 55 cases of Burkitt's lymphoma (BL) with respect to geography and Epstein-Barr virus (EBV) association. We have confirmed the association between chromosome 8 breakpoint and geography: 74% of endemic (eBL) but only 9% of sporadic BL (sBL) had breakpoints outside the HindIII fragment encompassing the c-myc gene (P2 less than .00001). Conversely, not only did 91% of sBL manifest a rearranged HindIII fragment, but at least 56% of these cases, in contrast to 17% of eBL cases, had a breakpoint within the first exon or intron of c-myc (P2 less than .004). Breakpoints outside the switch mu (S mu) region (ie, the HindIII fragment encompassing S mu) on chromosome 14 were twice as common overall (73%) as those within S mu (27%), but in the 15 tumors with S mu breakpoints, 13 (87%) had a rearranged c-myc gene. Breakpoints outside the HindIII fragment encompassing c-myc on chromosome 8 were predominantly associated with non-S mu breakpoints on chromosome 14 (85%) and this was the combination most frequently associated with eBL (65%; 6% of sBL, P2 less than .00001). In sBL, the most frequent breakpoint combination was a rearranged c-myc gene with a non-S mu breakpoint (63%; 13% of eBL). Twenty-eight percent of sBL and 13% of eBL had breakpoints both within c-myc and within S mu. EBV DNA was present in 19 of 20 tumors with breakpoints outside c-myc, in none of 7 with a breakpoint in the immediate 5′ region of c-myc, in 4 of 5 tumors with breakpoints in the first exon, and in 7 of 12 tumors with breakpoints in the first intron. These data suggest that the pathogeneses of eBL and sBL differ with regard to the mechanism of c-myc deregulation, and probably also with regard to the state of differentiation of the target cell for malignant transformation. We have formulated a testable hypothesis regarding the potential role of EBV in pathogenesis: that it is required to contribute to the deregulation of c-myc in the presence of some, but not all, types of c-myc damage arising from the chromosomal translocations.

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Shiramizu, B., F. Barriga, J. Neequaye, A. Jafri, R. Dalla-Favera, A. Neri, M. Guttierez, P. Levine et I. Magrath. « Patterns of chromosomal breakpoint locations in Burkitt's lymphoma : relevance to geography and Epstein-Barr virus association ». Blood 77, n^o 7 (1 avril 1991) : 1516–26. http://dx.doi.org/10.1182/blood.v77.7.1516.bloodjournal7771516.

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We have examined, by Southern blotting, the patterns of chromosomal breakpoint locations in 55 cases of Burkitt's lymphoma (BL) with respect to geography and Epstein-Barr virus (EBV) association. We have confirmed the association between chromosome 8 breakpoint and geography: 74% of endemic (eBL) but only 9% of sporadic BL (sBL) had breakpoints outside the HindIII fragment encompassing the c-myc gene (P2 less than .00001). Conversely, not only did 91% of sBL manifest a rearranged HindIII fragment, but at least 56% of these cases, in contrast to 17% of eBL cases, had a breakpoint within the first exon or intron of c-myc (P2 less than .004). Breakpoints outside the switch mu (S mu) region (ie, the HindIII fragment encompassing S mu) on chromosome 14 were twice as common overall (73%) as those within S mu (27%), but in the 15 tumors with S mu breakpoints, 13 (87%) had a rearranged c-myc gene. Breakpoints outside the HindIII fragment encompassing c-myc on chromosome 8 were predominantly associated with non-S mu breakpoints on chromosome 14 (85%) and this was the combination most frequently associated with eBL (65%; 6% of sBL, P2 less than .00001). In sBL, the most frequent breakpoint combination was a rearranged c-myc gene with a non-S mu breakpoint (63%; 13% of eBL). Twenty-eight percent of sBL and 13% of eBL had breakpoints both within c-myc and within S mu. EBV DNA was present in 19 of 20 tumors with breakpoints outside c-myc, in none of 7 with a breakpoint in the immediate 5′ region of c-myc, in 4 of 5 tumors with breakpoints in the first exon, and in 7 of 12 tumors with breakpoints in the first intron. These data suggest that the pathogeneses of eBL and sBL differ with regard to the mechanism of c-myc deregulation, and probably also with regard to the state of differentiation of the target cell for malignant transformation. We have formulated a testable hypothesis regarding the potential role of EBV in pathogenesis: that it is required to contribute to the deregulation of c-myc in the presence of some, but not all, types of c-myc damage arising from the chromosomal translocations.

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Mansfield, Shawn D., Greg S. Bezanson et Thomas J. Marrie. « Characterization and cloning of a 37.6-kb plasmid carried byLegionella pneumophilarecovered from patients and hospital water over a 12-year period ». Canadian Journal of Microbiology 43, n^o 2 (1 février 1997) : 193–97. http://dx.doi.org/10.1139/m97-025.

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For 12 years, strains of Legionella pneumophila serogroup 1 harbouring a 37.6-kb (23 MDa) plasmid have predominated among patient and potable water isolates at the Victoria General Hospital, Halifax, N.S. Plasmid DNA recovered from 24 strains isolated between 1983 and 1995 was digested with the restriction endonucleases EcoRI, HindIII, KpnI, PvuII, XbaI, and BamHI. The distribution of cutting sites indicated that the 23-MDa size group had remained essentially unchanged during this period, suggesting the persistence of a single plasmid type. Further fragmentation pattern analysis permitted the construction of a physical map of the prototype 23-MDA plasmid, pLp4269. Double digestion with BamHI–HindIII enabled the cloning of 94.4% of pLp4269 into pBluescript vector. A 2.1-kb fragment was not clonable. Plasmid pLp4269 is the first of the smaller Legionella extrachromosomal DNAs to be characterized in this way.Key words: Legionella, plasmid, stability, map, cloning.

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McFarland, Elizabeth J., Ruth A. Karron, Petronella Muresan, Coleen K. Cunningham, Charlotte Perlowski, Jennifer Libous, Jennifer Oliva et al. « Live-Attenuated Respiratory Syncytial Virus Vaccine With M2-2 Deletion and With Small Hydrophobic Noncoding Region Is Highly Immunogenic in Children ». Journal of Infectious Diseases 221, n^o 12 (1 février 2020) : 2050–59. http://dx.doi.org/10.1093/infdis/jiaa049.

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Abstract Background Respiratory syncytial virus (RSV) is the leading viral cause of severe pediatric respiratory illness, and vaccines are needed. Live RSV vaccine D46/NS2/N/ΔM2-2-HindIII, attenuated by deletion of the RSV RNA regulatory protein M2-2, is based on previous candidate LID/ΔM2-2 but incorporates prominent differences from MEDI/ΔM2-2, which was more restricted in replication in phase 1. Methods RSV-seronegative children aged 6–24 months received 1 intranasal dose (105 plaque-forming units [PFUs] of D46/NS2/N/ΔM2-2-HindIII [n = 21] or placebo [n = 11]) and were monitored for vaccine shedding, reactogenicity, RSV-antibody responses and RSV-associated medically attended acute respiratory illness (RSV-MAARI) and antibody responses during the following RSV season. Results All 21 vaccinees were infected with vaccine; 20 (95%) shed vaccine (median peak titer, 3.5 log10 PFUs/mL with immunoplaque assay and 6.1 log10 copies/mL with polymerase chain reaction). Serum RSV-neutralizing antibodies and anti-RSV fusion immunoglobulin G increased ≥4-fold in 95% and 100% of vaccines, respectively. Mild upper respiratory tract symptoms and/or fever occurred in vaccinees (76%) and placebo recipients (18%). Over the RSV season, RSV-MAARI occurred in 2 vaccinees and 4 placebo recipients. Three vaccinees had ≥4-fold increases in serum RSV-neutralizing antibody titers after the RSV season without RSV-MAARI. Conclusions D46/NS2/N/ΔM2-2-HindIII had excellent infectivity and immunogenicity and primed vaccine recipients for anamnestic responses, encouraging further evaluation of this attenuation strategy. Clinical Trials Registration NCT03102034 and NCT03099291.

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Girard-Santosuosso, O., I. Lantier, N. Millet, C. Mouline, J. F. Guillot, J. Protais, P. Colin, C. Beaumont et F. Lantier. « TaqI and HindIII RFLPs at the chicken PAX3 locus ». Animal Genetics 27, n^o 5 (24 avril 2009) : 374–75. http://dx.doi.org/10.1111/j.1365-2052.1996.tb00987.x.

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Chuchana, P., A. Blancher, G. Lefranc et M. P. Lefranc. « HindIII RFLP of the human immunoglobulin VλIII subgroup genes ». Nucleic Acids Research 18, n^o 20 (1990) : 6179. http://dx.doi.org/10.1093/nar/18.20.6179.

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Prior, T. W., K. J. Friedman et L. M. Silverman. « RFLP for HindIII at the Duchenne muscular dystrophy gene ». Nucleic Acids Research 17, n^o 6 (1989) : 2370. http://dx.doi.org/10.1093/nar/17.6.2370.

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Heinzmann, C., J. Ladias, S. Antonarakis, T. Kirchgessner, M. Schotz et A. J. Lusis. « RFLP for the human lipoprotein lipase (LPL) gene : HindIII ». Nucleic Acids Research 15, n^o 16 (1987) : 6763. http://dx.doi.org/10.1093/nar/15.16.6763.

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Yüksel, B., et A. H. Paterson. « Construction and characterization of a peanut HindIII BAC library ». Theoretical and Applied Genetics 111, n^o 4 (28 juillet 2005) : 630–39. http://dx.doi.org/10.1007/s00122-005-1992-x.

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Xie, Jianping, Hui Yun, Haigang Dong, Wenya Zhao, Guohua Wang, Guanzhou Qiu et Xinxing Liu. « Simultaneous extraction, separation and purification of microbial genomic DNA and total RNA from acidic habitat samples ». Analytical Methods 7, n^o 3 (2015) : 909–17. http://dx.doi.org/10.1039/c4ay01608d.

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DNA and RNA simultaneously extracted fromA. fusing the optimised method. (a) Total nucleic acid extracted fromA. f: lane M1, 1 kb ladder; lane M2,HindIII-cut lambda molecular size marker; lanes 1–4, biological replicates. (b) DNA precipitated by isopropanol. (c) RNA precipitated by LiCl.

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Терлецкий, Валерий Павлович, et Валентина Ивановна Тыщенко. « Выявление аллельных вариантов гена рецептора гормона роста у кур ». Аграрная Россия, n^o 7 (24 août 2019) : 30–33. http://dx.doi.org/10.30906/1999-5636-2019-7-30-33.

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Проведен молекулярно-генетический анализ генотипов племенных кур и петухов в популяции кур кросса «Смена-8». Кур и петухов (всего 96 голов) отбирали из племенного ядра. Представлены результаты выявления полиморфизмов в гене рецептора гормона роста. В качестве эндонуклеазы рестрикции использовали HindIII, которая выявляет мутацию с заменой нуклеотида G на A, что приводит к изменению размера амплификата. Размер продукта полимеразной цепной реакции (ПЦР) без расщепления ферментом составлял 718 пар оснований ДНК. Расщепление этого амплификата ферментом HindIII приводило к тому, что после проведения электрофореза выявляли генотип A1A1, который характеризовался фрагментами ДНК размером 428 и 290 пар оснований, и генотип A2A2 — 290, 258 и 170 пар оснований. В целом, генотип A2A2 преобладал в исследуемой популяции, количество особей составляло 80. Генотип A1A1 встречался у остальных 16 птиц. Среди 12 исследованных петухов генотип A1A1 отмечен только у 1 особи. Метод ПЦР дает возможность оценить генетическое разнообразие в популяции кур по аллелям гена рецептора гормона роста для дальнейшего использования этих данных в селекционной работе при отборе и подборе пар.

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Bonavent, J. F., L. Bessone, A. Geny, A. Berville, J. P. Denizot et C. Brian. « A possible origin for the sugar beet cytoplasmic male sterility source Owen ». Genome 32, n^o 2 (1 avril 1989) : 322–27. http://dx.doi.org/10.1139/g89-448.

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The cytoplasmic male sterility source of sugar beet used for hybrid seed production is distinguished from the maintainer (O type) by the HindIII chloroplast DNA (ctDNA) restriction pattern. We searched the genus Beta for a putative species carrying the same ctDNA as the Owen cytoplasmic male sterility source. Each of the three sections of this genus displays a specific HindIII profile for ctDNA. We, therefore, suggest the putative species be classified to the Vulgares section as the sugar beet. An old variety of the garden beet, "Crapaudine', in our collection carries the same ctDNA restriction profile as the Owen cytoplasmic male sterility source. This variety presumably was never crossed by breeders with either the Owen cytoplasmic male sterility line or the sugar beet. Our results, however, allow us to propose that the origin of the Owen cytoplasmic male sterility was through a cross of the 'Crapaudine' variety, as female, from the Semaphor company.Key words: cytoplasmique male sterility, sugar beet, chloroplast DNA, garden beet, wild species.

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Molnar, Stephen J., et George Fedak. « Polymorphism in ribosomal DNA repeat units of 12 Hordeum species ». Genome 32, n^o 6 (1 décembre 1989) : 1124–27. http://dx.doi.org/10.1139/g89-565.

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Total genomic DNA was isolated from leaves of individual accessions of 12 Hordeum species. SacI, HindIII, and BamHI restriction fragments hybridizing to the wheat rDNA probe pTA71 were compared. Thirteen rDNA repeat unit length variants were recovered, which combined to produce 11 distinct phenotypes. All rDNA repeat units had two SacI sites and no HindIII sites. The rDNA repeat units of H. secalinum, H. hrasdanicum, and H. distichum all had 3 BamHI sites, but in different configurations. The rDNA repeat units of the other species had at least 4 BamHI sites. Ladders of small hybridizing fragments in 8 of 9 of the latter accessions indicated the presence of occasional BamHI sites in some subrepeats within the intergenic spacer regions of the rDNA repeat units. Different distributions of BamHI restriction site maps within the Hordeum sections strengthen the concept of using variation in rDNA restriction sites as a taxonomic character.Key words: Hordeum, ribosomal DNA, polymorphism.

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