Littérature scientifique sur le sujet « Hibiscu »
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Articles de revues sur le sujet "Hibiscu"
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Tangkawanit, Sudaporn. « Eco-Friendly of Hibiscu cannabinus L. Fibers Dyeing with Natural Dye ». Advanced Materials Research 781-784 (septembre 2013) : 674–77. http://dx.doi.org/10.4028/www.scientific.net/amr.781-784.674.
Texte intégralRésumé :
This research was an intensive study on development of Hibiscus cannabinus L. fibers dyeing with natural dye. The result reflected that color shade and color strength value results were different depend on type of mordanted. The Hibiscus cannabinus L. washing with detergent dyeing with Caesalpinia sappan L. and various mordented showed that light fastness was in level 3-4, when CuSO4 and FeSO4 mordanted light fastness result was in level 4-5, mordants as lime, CuSO4 and FeSO4 were added washing fastness property was in level 5.
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Lima, Michele Vieira da Silva, Clarice de Morais Guedes, Maria Carolina de Abreu et Ana Paula Peron. « Análise da citotoxicidade e genotoxicidade de Hibiscus sabdariffa L. in natura e industrializado, e comparação da toxicidade entre as formas analisadas da planta. » Multitemas 23, no 55 (16 octobre 2018) : 121. http://dx.doi.org/10.20435/multi.v23i55.1838.
Texte intégralRésumé :
A flores de Hibiscus sabdariffa, o popular hibisco, são amplamente utilizadas para a prevenção e tratamento de diversas enfermidades. Comercialmente, tais flores são encontradas na forma in natura, onde são recomendadas para uso na forma de chá; e na forma industrializada, encontrada como produto farmacêutico acrescidos de aditivos excipientes. Objetivou-se na presente pesquisa avaliar em células meristemáticas de raízes de Allium cepa, nos tempos de exposição 24 e 48 horas, o potencial citotóxico e genotóxico de flores de hibisco em chá, nas concentrações 0,10; 0,20 e 0,40 mg/ml; e industrializadas, provenientes de dois diferentes laboratórios farmacêuticos, denominados neste estudo de A e B, nas concentrações 0,15; 0,30 e 0,60 mg/mL. A partir dos resultados obtidos, verificou-se que as três concentrações de chá de hibisco não foram citotóxicas e nem genotóxicas as células meristemáticas de raízes de A. cepa. No entanto, as três concentrações de hibisco referentes ao produto LF B, bem como as três concentrações do produto LF A, foram citotóxicas e genotóxicas, respectivamente, aos meristemas de raízes. Portanto, nas condições de estudos estabelecidas, verificou-se diferença de toxicidade celular entre os hibiscos in natura e os hibiscos industrializados.
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Barros de Nogueira, Ruama Viana, Virginia Freitas Rodrigues, Sílvia Menezes de Faria Pereira et Inêz Barcellos de Andrade. « FLORES DE HIBISCO (Hibiscus sabdariffa) COMERCIALIZADAS NO MUNICÍPIO DE CAMPOS DOS GOYTACAZES-RJ : UMA ANÁLISE DO PERFIL FITOQUÍMICO E DO USO POPULAR, CONSIDERANDO ATIVIDADES ATRIBUIDAS ÀS RESPECTIVAS CLASSES DE METABÓLIDOS SECUNDÁRIOS ENCONTRADAS ». Revista Científica da Faculdade de Medicina de Campos 16, no 1 (30 avril 2021) : 12–18. http://dx.doi.org/10.29184/1980-7813.rcfmc.253.vol.16.n1.2021.
Texte intégralRésumé :
As formas mais antigas para prevenção de doenças, tratamento e cura, estão na utilização das plantas medicinais. Dentre as várias espécies utilizadas pela população, encontra-se o Hibisco (Hibiscus sabdariffa). Pertencente à família botânica Malvaceae, o Hibisco é um arbusto anual, nativo dos continentes africano e asiático, que se encontra distribuído nas regiões tropicais e subtropicais de ambos os hemisférios, tornando-se naturalizado em muitas áreas das Américas. Estudos têm comprovado o uso de Hibisco como agente diurético, antimicrobiano, laxante, sedativo, anti-hipertensivo, entre outros. Mais recentemente, há indicativo de que o Hibisco possa agir como antioxidante, antimutagênico, antitumoral e antileucêmico. O presente trabalho teve como objetivo realizar uma análise fitoquímica preliminar nas flores de Hibiscus sabdariffa, comercializadas em Campos dos Goytacazes, RJ, e correlacionar os resultados obtidos com as atividades farmacológicas atribuídas às classes de metabólitos encontrados. O presente estudo revelou a presença de grande quantidade (52,09%) de metabólitos de alta polaridade nas flores de Hibisco. Análise fitoquímica de identificação revelou a presença do metabólito secundário da classe dos taninos, nas flores analisadas da espécie vegetal (Hibiscus sabdariffa). A análise de identificação de flavonoides, por sua vez, não permitiu confirmar a presença desse metabólito visto que a coloração da própria espécie mascarou o resultado. As atividades atribuídas, em referências bibliográficas, à espécie Hibiscus sabdariffa, em especial antimicrobiana e antibiótica, foi compatível com as atividades relacionadas à classe de metabólitos identificada na amostra. Tal estudo revela um resultado preliminar, mas pode nortear outros estudos na busca de substâncias com atividade antioxidante.
As formas mais antigas para prevenção de doenças, tratamento e cura, estão na utilização das plantas medicinais. Dentre as várias espécies utilizadas pela população, encontra-se o Hibisco (Hibiscus sabdariffa). Pertencente à família botânica Malvaceae, o Hibisco é um arbusto anual, nativo dos continentes africano e asiático, que se encontra distribuído nas regiões tropicais e subtropicais de ambos os hemisférios, tornando-se naturalizado em muitas áreas das Américas. Estudos têm comprovado o uso de Hibisco como agente diurético, antimicrobiano, laxante, sedativo, anti-hipertensivo, entre outros. Mais recentemente, há indicativo de que o Hibisco possa agir como antioxidante, antimutagênico, antitumoral e antileucêmico. O presente trabalho teve como objetivo realizar uma análise fitoquímica preliminar nas flores de Hibiscus sabdariffa, comercializadas em Campos dos Goytacazes, RJ, e correlacionar os resultados obtidos com as atividades farmacológicas atribuídas às classes de metabólitos encontrados. O presente estudo revelou a presença de grande quantidade (52,09%) de metabólitos de alta polaridade nas flores de Hibisco. Análise fitoquímica de identificação revelou a presença do metabólito secundário da classe dos taninos, nas flores analisadas da espécie vegetal (Hibiscus sabdariffa). A análise de identificação de flavonoides, por sua vez, não permitiu confirmar a presença desse metabólito visto que a coloração da própria espécie mascarou o resultado. As atividades atribuídas, em referências bibliográficas, à espécie Hibiscus sabdariffa, em especial antimicrobiana e antibiótica, foi compatível com as atividades relacionadas à classe de metabólitos identificada na amostra. Tal estudo revela um resultado preliminar, mas pode nortear outros estudos na busca de substâncias com atividade antioxidante.
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Abreu, Bruna Barbosa, Cintya Regina Nunes Sousa, Juliana Carvalho Passos, Anne Rafaele Silva Marinho, Amanda Castro Amorim Serpa Brandão et Regilda Saraiva dos Reis Moreira-Araújo. « Composição centesimal, compostos bioativos e atividade antioxidante em cálice de hibisco (Hibiscus sabdariffa L.) ». Jornal Interdisciplinar de Biociências 4, no 1 (13 janvier 2020) : 1. http://dx.doi.org/10.26694/jibi.v4i1.7618.
Texte intégralRésumé :
Hibiscus sabdariffa L. é uma planta originária da Índia, do Sudão e da Malásia. Tendo em vista a busca de alimentos ricos em compostos fenólicos e consequentemente com alta atividade antioxidante, o hibisco apresenta-se como uma planta com alta perspectiva. Neste contexto, estudos sobre a caracterização completa de seus componentes podem ampliar o âmbito de aplicações do hibisco, portanto, o presente trabalho tem como objetivo determinar a composição centesimal, compostos bioativos e atividade antioxidante em cálice de Hibiscus sabdariffa L. As análises foram realizadas em duplicata no Laboratório de Bromatologia e Bioquímica dos Alimentos e Sala de Antioxidantes da Universidade Federal do Piauí. Os cálices utilizados como amostras na referida pesquisa foram adquiridos no comercio varejista de Teresina-PI. Foram determinados teores de umidade, cinzas, lipídeos, proteínas, carboidratos, valor energético total, compostos fenólicos totais, flavonóides totais, carotenoides, antocianinas, ácido ascórbico, proantocianidinas e atividade antioxidante. Os teores de compostos fenólicos totais e atividade antioxidante em H. sabdariffa L. foram respectivamente iguais a 429,84 ± 5,16 (EAG/100g) e 626,32 ± 8,03 (μmol trolox/L). Concluiu-se que o cálice de hibisco possui altos teores de cinzas totais, carboidratos, fenólicos totais e atividade antioxidante, bem como baixos teores de lipídeos e proteínas.
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SANTOS, B. S., L. C. O. BARRETTO, J. A. B. SANTOS et G. F. SILVA. « OBTENTION, FREEZE-DRYING AND CHARACTERIZATION OF LEMON GRASS (Cymbopogon citratus D.C.) AND HIBISCUS (Hibiscus sabdariffa L.) EXTRACTS ». Revista Gestão, Inovação e Tecnologias 3, no 5 (27 janvier 2014) : 090–99. http://dx.doi.org/10.7198/s2237-0722201300050008.
Texte intégral
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Cabrera, María G., Roberto E. Álvarez, Susana A. Gutiérrez, María A. Cúndom et Angela A. Sosa López. « Enfermedades que afectan a las plantas de hibiscus cultivadas como ornamentales en Corrientes ». Agrotecnia, no 21 (25 août 2013) : 30. http://dx.doi.org/10.30972/agr.021333.
Texte intégralRésumé :
En la provincia de Corrientes se observó que muchos ejemplares de Hibiscus cultivados como ornamentales, presentaban enfermedades que afectaban su estética y debilitaban las plantas. Se recolectaron para su estudio muestras de Sinencia o Rosa de la China (Hibiscus rosa-sinensis L.), rosa de Jamaica o de Abisinia (Hibiscus sabdariffa L.), rosa de Siria (Hibiscus syriacus L.) y ejemplares de Abutilon x hybridum hort., con el objeto de reconocer que patógenos afectaban a estas especies de Malvaceae en la provincia de Corrientes y caracterizar las enfermedades que provocaban. En algunos casos los síntomas afectaban a las hojas y en otros a toda la parte aérea de las plantas con manifiestas alteraciones. El objetivo del presente trabajo fue identificar al agente causal de cada enfermedad de las especies ornamentales de los géneros Hibiscus y Abutilon presentes en viveros, plazas y paseos ubicados en varias localidades de la provincia de la Corrientes. Se siguieron las técnicas usuales de laboratorio para el estudio de estas enfermedades y sus agentes causales y revisión de bibliografía. Se determinaron: el agente causal de antracnosis, Colletotrichum gloeosporioides; el causante de la cercosporiosis Cercospora hibisci; una roya causada por una cepa de Cerospsora sp. y también se observaron sintomatologías similares a virus como las causadas por Hibiscus chlorotic ringspot carmovirus (HCRSV) y Abutilon mosaic virus Morren (AMV) sobre H. rosa-sinensis y H. syriacus.
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Abouzeid, Fatma M., et Sultanah Alshammery. « The Hibiscus extract Potential in Inhibiting Anodic Dissolution of Copper ». Revista de Chimie 73, no 3 (29 juillet 2022) : 67–81. http://dx.doi.org/10.37358/rc.22.3.8536.
Texte intégralRésumé :
Copper electro dissolution in H3PO4 acid is examined in solution containing different concentration of Hibiscus extract (Hibiscuss abdariffa). Potential of anode limiting current relation were computed and estimate of gently raising Hibiscus extract concentration (500-2000 ppm), The results reveal that Hibiscus extraction as a natural product has strong retardation behavior ranging from 24.5 � 42.1 % and the thermodynamic factors, adsorption isotherm and dimensionless parameters were given. Copper dissolution behavior in Hibiscus extract containing solution was investigated below ordinary convection and rotating cylinder electrode (RCE) as a compulsory convection. The rotating cylinder electrode RCE speed was investigated. The limiting current increases with increasing rotation, which indicates that the anodic corrosion is a diffusion-controlled process. The limiting current were reduced with raising extract plant concentration and raise with amplifying temperature from 293 -313 K). The activation energy values established which reaction rate was controlled via diffusion. Hibiscus extract adsorption follow Flory Huggins and kinetic thermodynamic models. The result at several circumstance was commanded via dimensionless correlations Sherwood (Sh), Schmidt (Sc) and Reynolds (Re) numbers.
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Lachance, Marc-André, Carlos A. Rosa, William T. Starmer, Birgit Schlag-Edler, J. Stuart F. Baker et Jane M. Bowles. « Metschnikowia continentalisvar.borealis,Metschnikowia continentalisvar.continentalis, andMetschnikowia hibisci, new heterothallic haploid yeasts from ephemeral flowers and associated insects ». Canadian Journal of Microbiology 44, no 3 (1 mars 1998) : 279–88. http://dx.doi.org/10.1139/w97-148.
Texte intégralRésumé :
Several strains of three new taxa of haploid heterothallic yeasts have been isolated from various ephemeral flowers and associated insects in North and South America and Australia. Metschnikowia continentalis comprises two varieties and is a close relative of Metschnikowia hawaiiensis. Like the latter, it produces giant ascospores and lives in association with the insects that colonize flowers of the family Convolvulaceae. These species exhibit an unusual asymmetrical mating, but their rare asci are sterile. The varieties of M. continentalis undergo unlimited mating, but ascospores are rarely formed. Metschnikowia continentalis var. continentalis was isolated in central Brazil and is thought to occur across South America.Metschnikowia continentalis var. borealis was recovered in the Great Lakes area and may represent a North American population. Metschnikowia hibisci was found in the flowers and insects of various Hibiscus species in the Australian states of New South Wales and Queensland but appeared to be absent in members of the Convolvulaceae growing in the same areas. The latter forms intermediate-sized ascospores and one of its mating types forms conjugation tubes in the presence of cells of other Metschnikowia species. The three taxa share with M. hawaiiensis a large deletion in the D2 region of their large ribosomal DNA subunit, but in M. hibisci, the variable domain of the D2 region shares little, if any, sequence similarity with others. The type cultures are as follows:M. continentalis var. continentalis strains UFMG96-173 (h+, CBS8429) and UFMG96-179 (h-, CBS8430); M. continentalis var. borealis strains UWO(PS)96-104.2 (h+, CBS 8431) and UWO(PS)96-101.1 (h-, CBS8432); and M. hibisci strains UWO(PS)95-797.2 (h+, CBS8433) and UWO(PS)95-805.1 (h-, CBS8434).Key words: Metschnikowia, yeast, Convolvulaceae, Hibiscus, geographic speciation.
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Araújo, Amanda Leandro, Julia Felipe Dias, Melissa Vitória Neto Fernandes, Thaís Leandro Rocha et Alexandre De Jesus Barros. « DESENVOLVIMENTO DE CÉLULAS SOLARES SENSIBILIZADAS POR CORANTE DE HIBISCO (HIBISCUS SABDARIFFA) ». Revista Conhecer Científico 1, no 3 (2022) : 1–5. http://dx.doi.org/10.56637/rcc.v1n3-002.
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Barros, Nara Vanessa dos Anjos, Joana Carvalho Serra, Rosielle Alves de Moura, Paulo Víctor de Lima Sousa et Regina Márcia Soares Cavalcante. « Desenvolvimento de Massa de Pizza Enriquecida com Hibisco ». Ensaios e Ciência C Biológicas Agrárias e da Saúde 24, no 5-esp. (18 février 2021) : 504–10. http://dx.doi.org/10.17921/1415-6938.2020v24n5-esp.p504-510.
Texte intégralRésumé :
Atualmente, busca-se constantemente o desenvolvimento de novos produtos para atender as necessidades dos consumidores, sendo a utilização do hibisco (Hibiscus sabidariffa L.) na produção alimentícia uma forma simples e econômica de agregar sabor aos produtos e beneficiar a saúde de quem os consome. O presente trabalho teve como objetivo desenvolver uma massa de pizza enriquecida com farinha de folhas de hibisco (FFH). Adicionou-se diferentes concentrações da FFH às massas de pizza, as quais foram submetidas às análises sensoriais e físico-químicas. Não houve diferença estatística significativa entre a formulação I (massa de pizza enriquecida com 2% de FFH) e a formulação II (massa de pizza enriquecida com 4% de FFH) em relação à aceitação global. Para as análises físico-químicas, as formulações obtiveram teores baixos de umidade (F1 - 18,07 ± 1,07; F2 - 19,3 ± 0,31) e um bom conteúdo de cinzas (F1 - 1,45 ± 0,06; F2 - 1,57 ± 0,10). Para o pH e acidez, os resultados obtidos estão de acordo com o preconizado pela legislação vigente, com resultados que variaram de 5,0 a 6,0 para o pH, e de 1,7 a 2,8, para a acidez total titulável. Constatou-se que ambas amostras obtiveram boa aceitação por parte dos provadores, assim como uma intenção de compra satisfatória. Em relação às análises físico-químicas, verificou-se que os produtos desenvolvidos obtiveram características semelhantes a outras massas enriquecidas, estando de acordo com o preconizado nas legislações para este produto.
Palavras-chave: Desenvolvimento de Produtos. Hibiscus sabidariffa L. Produto de Panificação.
Abstract
Currently, there is a constant search for the development of new products to meet the consumer’s needs, and the use of hibiscus (Hibiscus sabidariffa L.) in food production is a simple and economical way to add flavor to products and benefit the health of those who consume them. The present work had as objective to develop pizza dough enriched with hibiscus leaf flour (HLF). Different concentrations of HLF were added to the pizza masses, which were submitted to the sensorial and physicochemical analyzes. There was no statistically significant difference between formulation I (2% HLF enriched pizza mass) and formulation II (4% HLF enriched pizza mass) in relation to the overall acceptance. For the physicochemical analysis, the formulations obtained low levels of humidity (F1 - 18.07 ± 1.07; F2 - 19.3 ± 0.31) and a good ash content (F1 - 1.45 ± 0, 06; F2 - 1.57 ± 0.10). For pH and acidity, the results obtained are in accordance with the recommendations of the current legislation, with results ranging from 5.0 to 6.0 for pH, and from 1.7 to 2.8, for total titratable acidity. It was found that both samples obtained good acceptance by the tasters, as well as a satisfactory purchase intention. Regarding the physical-chemical analysis, it was found that the products developed obtained characteristics similar to other enriched masses, being in accordance with the recommendations in the legislation for this product.
Keywords: Product Development. Hibiscus Sabidariffa L. Bakery Product.
Thèses sur le sujet "Hibiscu"
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MALACRIDA, ALESSIO. « EVALUATION OF ANTITUMORAL EFFECTS OF HIBISCUS SABDARIFFA ON MULTIPLE MYELOMA CELLS ». Doctoral thesis, Università degli Studi di Milano-Bicocca, 2017. http://hdl.handle.net/10281/158152.
Texte intégralRésumé :
Hibiscus Sabdariffa (HS) is a plant of the Malvacee family commonly cultured in tropical and subtropical countries. It is mainly known as the main ingredient for the preparation of cold drink called Karkadè. Calices and leaves of HS plant are also used in folk medicine thanks to their antioxidant and anti-inflammatory properties. In recent years, HS has also gained great interest as a possible antitumoral agent.
In the present PhD project, we evaluated the antitumoral effects of HS against multiple mye-loma cells in vitro. Multiple myeloma is the most frequent hematological malignancy world-wide. In recent years, new drugs have increased the survival expectancy of patients. Despite this, new therapeutic approaches are necessary, especially for high multiple myeloma hetero-geneity and for relapsed or refractory multiple myeloma.
The project was organized in three distinct phases:
1- Evaluation of antitumoral effects of HS against RPMI 8226 human multiple myeloma cells. We demonstrated by MTT and Trypan blue assays that a total HS extract (HSE) and one of its fraction obtained by liquid-liquid extraction (HSEC) were able to impair cell viability of human multiple myeloma RPMI 8226 in a dose and time dependent manner. HSE cell viability reduction was due to a cytostatic action, while HSEC was more cytotoxic and induced a caspase dependent apoptosis. Moreover, both HSE and HSEC impaired cell migration and invasion of RPMI 8226 cells in a Boyden chamber as-say. We also demonstrated in in vitro model of neurotoxicity (dorsal root ganglia model) that HSE and HSEC concentrations used in our experiments were not neurotoxic. In RPMI 8226 cells autophagy and proteasome activity were impaired by both HSE and HSEC. MAPK p38 activation was observed in the first 6h of treatment, while ERK 1 and ERK 2 activation occurred between 16 and 48h.
2- Evaluation of combinations between Bortezomib (BTZ) and HSE or HSEC against RPMI 8226 multiple myeloma cells. We evaluated several combinations between BTZ and HSE or HSEC (simultaneous, not-simultaneous treatment and pretreatment) using MTT assay to assess their effectiveness. Among all evaluated combinations, only the 24h BTZ 1nM pretreatment followed by HSE or HSEC treatment resulted more effective than BTZ or HSE or HSEC single treatment in reducing cell viability. This combination was not neurotoxic in the dorsal root ganglia model.
3- Isolation and characterization of HSEC molecules responsible of the antitumoral ef-fect. Using a bioguided method, we isolated several fractions from HSEC. Fractions were obtained by flash column chromatography or by resin use. Molecular characterization was performed by HPLC, NMR or mass spectrometry, while biological activity was screened in human multiple myeloma RPMI 8226 cells by MTT and proteasome activity assay. We isolated three fractions with a first fractionating process that keep their activ-ity on RPMI 8226 cells, but that are not completely pure. In a second fractionating, we isolated a highly pure fraction with a significant activity on RPMI 8226 cell viability. We also characterized this fraction by both a molecular and biological point of view.
In conclusion, we demonstrated the antitumoral effect of HS in vitro against RPMI 8226 multiple myeloma cells. We also found a new therapeutic combination between BTZ and HS that enhanced their antitumoral effect when compared to single treatments. Moreover, we also isolated one of the molecules that are involved in the antitumoral effect of HS.
The results of my PhD project showed that HS could be a promising chemotherapeutic agent, but further studies are still needed.Hibiscus Sabdariffa (HS) is a plant of the Malvacee family commonly cultured in tropical and subtropical countries. It is mainly known as the main ingredient for the preparation of cold drink called Karkadè. Calices and leaves of HS plant are also used in folk medicine thanks to their antioxidant and anti-inflammatory properties. In recent years, HS has also gained great interest as a possible antitumoral agent. In the present PhD project, we evaluated the antitumoral effects of HS against multiple mye-loma cells in vitro. Multiple myeloma is the most frequent hematological malignancy world-wide. In recent years, new drugs have increased the survival expectancy of patients. Despite this, new therapeutic approaches are necessary, especially for high multiple myeloma hetero-geneity and for relapsed or refractory multiple myeloma. The project was organized in three distinct phases: 1- Evaluation of antitumoral effects of HS against RPMI 8226 human multiple myeloma cells. We demonstrated by MTT and Trypan blue assays that a total HS extract (HSE) and one of its fraction obtained by liquid-liquid extraction (HSEC) were able to impair cell viability of human multiple myeloma RPMI 8226 in a dose and time dependent manner. HSE cell viability reduction was due to a cytostatic action, while HSEC was more cytotoxic and induced a caspase dependent apoptosis. Moreover, both HSE and HSEC impaired cell migration and invasion of RPMI 8226 cells in a Boyden chamber as-say. We also demonstrated in in vitro model of neurotoxicity (dorsal root ganglia model) that HSE and HSEC concentrations used in our experiments were not neurotoxic. In RPMI 8226 cells autophagy and proteasome activity were impaired by both HSE and HSEC. MAPK p38 activation was observed in the first 6h of treatment, while ERK 1 and ERK 2 activation occurred between 16 and 48h. 2- Evaluation of combinations between Bortezomib (BTZ) and HSE or HSEC against RPMI 8226 multiple myeloma cells. We evaluated several combinations between BTZ and HSE or HSEC (simultaneous, not-simultaneous treatment and pretreatment) using MTT assay to assess their effectiveness. Among all evaluated combinations, only the 24h BTZ 1nM pretreatment followed by HSE or HSEC treatment resulted more effective than BTZ or HSE or HSEC single treatment in reducing cell viability. This combination was not neurotoxic in the dorsal root ganglia model. 3- Isolation and characterization of HSEC molecules responsible of the antitumoral ef-fect. Using a bioguided method, we isolated several fractions from HSEC. Fractions were obtained by flash column chromatography or by resin use. Molecular characterization was performed by HPLC, NMR or mass spectrometry, while biological activity was screened in human multiple myeloma RPMI 8226 cells by MTT and proteasome activity assay. We isolated three fractions with a first fractionating process that keep their activ-ity on RPMI 8226 cells, but that are not completely pure. In a second fractionating, we isolated a highly pure fraction with a significant activity on RPMI 8226 cell viability. We also characterized this fraction by both a molecular and biological point of view. In conclusion, we demonstrated the antitumoral effect of HS in vitro against RPMI 8226 multiple myeloma cells. We also found a new therapeutic combination between BTZ and HS that enhanced their antitumoral effect when compared to single treatments. Moreover, we also isolated one of the molecules that are involved in the antitumoral effect of HS. The results of my PhD project showed that HS could be a promising chemotherapeutic agent, but further studies are still needed.
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Ndiaye, Oumoule. « Impacts of Water, Extraction Procedure and Origin on Anthocyanins and Volatile Compositions of Hibiscus Extracts and Freeze-Dried Hibiscus ». Thesis, Virginia Tech, 2016. http://hdl.handle.net/10919/88756.
Texte intégralRésumé :
There has been a lot of interest in Roselle (Hibiscus sabdariffa L.), called Bissap in Senegal, hibiscus recently because of consumer interest in nutraceutical products. However, beverages made from hibiscus have a short self-life due to anthocyanin and flavor degradation. The purpose of our study was first to assess the impacts of water, extraction procedure and origin on the anthocyanins of hibiscus extracts and secondly, to examine the impacts of freeze-drying on the anthocyanins and the volatiles compositions of hibiscus extracts. For the first experiment, a 2x3 factorial design was used with hibiscus calyces from Senegal and Egypt for the factor origin, distilled water and reformulated Dakar (Senegal) water for the second factor water, and then cold and hot extraction procedures were applied. For the second experiment, Senegalese hibiscus was extracted with hot and cold water and one part of each extract was freeze-dried. For both objectives, a ratio of 1:15 w/v (1 kilogram of calyces for 15 liters of water) were used. The time-temperature was 98°C / 30 min for hot and 22°C / 4 hours for cold extractions. The anthocyanins were determined using high performance liquid chromatography (HPLC). And the volatiles were measured using headspace-solid phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GCMS). Origin and temperature as well as their interaction had significant effects on the anthocyanin contents, with respective p-values of 0.0036 and 0.0025 and 0.0002. Freeze-drying showed no effect on the anthocyanins in cold extracts. In contrast, a significant difference between the hot extract and its freeze-dried product was observed with a p-value of 0.0013. For the flavor compounds, the aroma profiles were different between cold and hot extracts and their instant powders. Globally the results of this study can help in the optimization when processing hibiscus derivatives.MSLFS
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Silva, Analú Barbosa da. « Caracterização antibacteriana, química e fitoquímica de flores de Hibiscus rosa-sinensis L. (mimo-de-vênus) e Hibiscus syriacus L. (hibisco-da-síria) como fonte de alimento ». reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/101498.
Texte intégralRésumé :
O Hibiscus rosa-sinensis L. e o Hibiscus syriacus L., da família Malvaceae são utilizados na área ornamental, mas nos últimos anos vem ganhando espaço na área alimentícia como flores comestíveis. Alguns estudos demonstram o potencial antibacteriano destas variedades frente a diversos microrganismos e sobre sua composição nutricional e fitoquímica há poucas pesquisas. Este trabalho teve por objetivo analisar a intensidade de atividade de inibição (IINIB) e a inativação bacteriana (IINAB) in vitro dos dois extratos alcoólicos das flores dos hibiscos e a relação com os polifenóis e antocianinas, e quantificar os compostos nutricionais e bioativos comparados com a atividade antioxidante. Avaliou-se a ação antibacteriana frente às bactérias de interesse alimentar, Staphylococcus aureus e Salmonella Enteretidis resultando em diferenças significativas entre as médias dos valores arbitrários (IINIB/IINAB). Observou-se a resistência da primeira bactéria com a segunda respectivamente em ambos os extratos vegetais. O doseamento dos compostos fitoquímicos presentes constatou que as plantas possuem correlação com a atividade antibacteriana e as propriedades químicas demonstraram valores significativos do ponto de vista nutricional e detectou-se efeito relevante com a atividade antioxidante.The Hibiscus rosa-sinensis L. and Hibiscus syriacus L., Malvaceae the family are used in the ornamental area, but in recent years has been gaining ground in the food area as edible flowers. Some studies have demonstrated the antibacterial potential of these varieties against various microorganisms and on their nutritional composition and phytochemical little research. This study aimed to analyze the intensity of activity inhibition (IINIB) and bacterial inactivation (IINAB) in vitro of both alcoholic extracts of petals of flowers of hibiscus and relationship with polyphenols and anthocyanins, and quantify the nutritional and bioactive compounds compared with antioxidant activity. We evaluated the antibacterial action on the bacteria of food interest, Staphylococcus aureus and Salmonella Enteretidis resulting in significant differences between the means of arbitrary values (IINIB/IINAB), where there was resistance from the first to the second bacterium in both extracts vegetables. The determination of phytochemical compounds found that plants have a correlation with the antibacterial activity and chemical properties showed significant amounts of nutritional standpoint and significant effect was detected with antioxidant activity.
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Cassol, Liliana. « Extração de compostos bioativos do hibisco (Hibiscus sabdariffa L.) por micro-ondas e seu encapsulamento por atomização e liofilização ». reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2018. http://hdl.handle.net/10183/179787.
Texte intégralRésumé :
Os cálices do hibisco possuem uma grande quantidade de compostos bioativos responsáveis pela sua atividade antioxidante. O presente trabalho teve como objetivos a obtenção de extratos contendo esses compostos bioativos em solução aquosa com 2 % de ácido cítrico por extração assistida por micro-ondas (EAM) e o encapsulamento desses extratos por atomização e liofilização utilizando polidextrose (PD), proteína isolada do soro de leite (WPI) e a mistura destes na concentração de 10 %. Previamente foram estudados três métodos de extração, o primeiro usando somente EAM a 200, 300 e 700 W de potência, e tempos de 2, 5 e 8 minutos; o segundo consistiu de dois períodos, a extração aquosa ácida com tempos de 1, 2, 4, 6, 18 e 24 horas seguida de EAM nas potências de 200, 300 e 700 W; o terceiro consistiu de EAM seguida de extração aquosa ácida, nos mesmos tempos e potências citados para o segundo método. Os resultados indicaram que a melhor condição do primeiro método foi a 700 W e 8 min, do segundo método realizada a 6 horas de extração aquosa ácida, seguida de EAM a 700 W por 8 min e do terceiro método, EAM a 700 W por 8 min seguida de 6 horas de extração aquosa ácida. Quando os três métodos foram comparados, a melhor condição de extração foi aquela obtida no terceiro método: 1,63 mg delfinidina-3-sambubiosídeo · g-1; 29,62 mg EAG · g-1; 133,25 μmol ET · g-1 para antocianinas, fenólicos totais e atividade antioxidante por ABTS, respectivamente. Para avaliar o efeito da extração obtida somente por EAM, os extratos obtidos por extração exaustiva com metanol por 25 min e EAM a 700 W e 8 min foram quantificados por HPLC-DAD-ESI-MS/MS, sendo encontrados 13 compostos (6 ácidos fenólicos, 2 antocianinas e 5 flavonóides derivados da quercetina, kaempferol e miricetina). Os compostos fenólicos majoritários foram o ácido 3-cafeoilquínico (2,58 e 1,32 mg · g-1) e ácido 5-cafeoilquínico (1,71 e 0,90 mg · g-1) para extração exaustiva e EAM, respectivamente. Esse mesmo extrato (700 W e 8 min) foi encapsulado por atomização (160 °C) e liofilização (- 68 °C por 54 horas). Os pós obtidos foram avaliados quanto aos teores de compostos fenólicos totais, antocianinas monoméricas totais, atividade antioxidante (ABTS, DPPH e HRSA), medidas por análises espectrofotométricas, atividade de água, umidade, higroscopicidade, solubilidade, eficiência de encapsulação, cor, análise termogravimétrica, temperatura de transição vítrea, espectroscopia de infravermelho com transformada de Fourier (FTIR) e microestrutura (MEV). Os pós atomizados tiveram menor atividade de água (0,14 a 0,17), umidade (3,4 a 4,5 %), higroscopicidade (23,9 a 34,1 %), solubilidade (86 a 98,2 %) e eficiência de encapsulação (51,62 a 84,52 %) do que os pós liofilizados. Os resultados do FTIR mostraram que os encapsulantes não interagiram quimicamente, visto que não foram observados mudanças na frequência dos picos; as provas termogravimétricas indicaram que os pós apresentaram a mesma tendência nas perdas de massa. Na análise de microestrutura foi observado um melhor desempenho nas micropartículas atomizadas com PD, as quais mostraram partículas mais esféricas e sem tendência de atração e aderência entre si. Foram obtidas retenções de 38 a 77 % para antocianinas monoméricas totais, 42 a 89 % para compostos fenólicos totais, e entre 33 e 90 % para atividade antioxidante nos pós obtidos. O pó encapsulado liofilizado com 10 % de polidextrose mostrou uma maior retenção de antocianinas (77 %), atividade antioxidante por DDPH (90 %) e HRSA (74 %), entretanto com maior higroscopicidade (39,4 %). As provas aceleradas de estocagem (umidades relativas de 75 e 90 % em temperaturas de 40 e 60 °C) realizadas em todos os pós encapsulados, após 30 dias, indicaram que o tratamento liofilizado com 10 % de PD foi o que apresentou melhores resultados a essas condições, retendo 75 % dos compostos fenólicos, com atividades antioxidantes medidas por ABTS, DPPH e HRSA de 75, 90 e 74 %, respectivamente, existentes no extrato original. O pó obtido tem potencial para aplicação em alimentos, portanto, devido ao hibisco ser uma matriz com ampla composição de compostos bioativos.The hibiscus calyces contend a high quantity of bioactive compounds responsible for their antioxidant activity. The present paper was aimed the production of extracts containing those bioactive compounds in acidified aqueous solution 2 % of citric acid by microwave assisted extraction (MAE) and the encapsulation of those extracts by spray drying and freeze-drying using polydextrose (PD), whey protein isolate (WPI) and their mixture in the concentration of 10 %. Previously three methods of extraction were studied, the first using only MAE at 200, 300 and 700 W of power, and times of 2, 5 and 8 minutes; the second consisted of two steps, the acid aqueous extraction with times of 1, 2, 4, 6, 18 and 24 hours followed by MAE at the powers of 200, 300 and 700 Watts; the third consisted of MAE followed by acid aqueous extraction, in the same times and powers mentioned for the second method. The results indicated that the best condition of the first method was 700 W and 8 minutes, the second method performed at 6 hours of acid aqueous extraction, followed by MAE at 700 W for 8 minutes and the third method, MAE at 700 W for 8 minutes followed by 6 hours of acid aqueous extraction. When the three methods are compared, the best condition of extraction was obtained in the third method: 1.63 mg delphinidin-3-sambubioside · g-1; 29.62 mg GAE · g-1; 133.25 μmol TE · g-1 for total monomeric anthocyanins, total phenolic compounds and antioxidant activity by ABTS, respectively. To evaluate the effect of the extraction obtained only by MAE, the extracts obtained by exhaustive extraction with methanol for 25 minutes and MAE at 700 W and 8 minutes were quantified by HPLC-DAD-ESI-MS/MS, was found 13 compounds (6 phenolic acids, 2 anthocyanins and 5 flavonoids derived from quercetin, kaempferol and myricetin). The phenolic compounds majorities were acid 3-caffeoylquinic (2.58 e 1.32 mg · g-1) and acid 5-caffeoylquinic (1.71 e 0.90 mg · g-1) for exhaustive extraction and MAE, respectively. That same extract (700 W and 8 minutes) was encapsulated by spray drying (160 ºC) and freeze-drying (- 68 °C for 54 hours). The obtained powders were evaluated about the levels of total phenolic compounds, total monomeric anthocyanins, antioxidant activity (ABTS, DPPH e HRSA), measured by spectrophotometric analysis, water activity, moisture, hygroscopicity, solubility, encapsulation efficiency, color, thermogravimetric analysis, glass transition temperature, Fourier transform infrared spectroscopy (FTIR) and microstructure (MEV). The spray dried powders had lower water activity (0.14 to 0.17), moisture (3.4 to 4.5 %), hygroscopicity (23.9 to 34.1 %), solubility (86 to 98.2 %) and encapsulation efficiency (51.62 to 84.52 %) than the freeze-dried powders. The results of FTIR showed that the encapsulants did not interact chemically, since changes were not observed on the frequency of the peaks; the thermogravimetric tests indicated that the powders presented the same tendency on the mass loss. On the microstructure analysis a better perform was observed on the spray dried microparticles with PD, which showed more spherical particles and with no tendency of attraction and adherence between them. Were obtained retentions of 38 to 77 % for total monomeric anthocyanins, 42 to 89 % for total phenolic compounds and between 33 and 90 % for antioxidant activity in the obtained powders. The encapsulated power by freeze-drying, with 10 % of polydextrose, was showed higher retention of anthocyanins (77 %), antioxidant activity by DDPH (90 %) and HRSA (74 %), however with higher hygroscopicity (39.4 %). The accelerated tests of storage (relative humidity of 75 and 90 % in temperatures of 40 and 60 ºC) performed in all the encapsulated powders, after 30 days, indicated that the freeze-drying treatment with 10 % of PD has the best behavior in those conditions, retaining 75 % of the phenolic compounds, with antioxidant activities measured by ABTS, DPPH and HRSA of 75, 90 and 74 %, respectively, present in the original extract. The obtained powder has potential for application in foods, therefore, due to the hibiscus being a matrix with ample composition of bioactive compounds.
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Maciel, Mônica Jachetti. « Avaliação do extrato alcoólico de hibisco (hibiscus sabdariffa l.) como fator de proteção antibacteriana e antioxidante em alimentos ». reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2011. http://hdl.handle.net/10183/29544.
Texte intégralRésumé :
O hibisco (Hibiscus sabdariffa L.) além de possuir propriedades antioxidantes e antimicrobianas, é utilizado como planta medicinal e alimento funcional nos países tropicais. Através de Testes de Diluição em Sistema de Tubos Múltiplos determinou-se a Intensidade de Atividade de Inibição Bacteriana (IINIB/Bacteriostasia) e a Intensidade de Atividade de Inativação Bacteriana (IINAB/Bactericidia) de extrato alcoólico de dois acessos de hibisco, a saber: Palmares do Sul/RS e Porto Alegre/RS sobre inóculos padronizados de Enterococcus faecalis (ATCC 19433), Escherichia coli (ATCC 11229), Salmonella Enteritidis (ATCC 11076) e Staphylococcus aureus (ATCC 25923). Paralelamente, o teor de polifenóis totais e de antocianinas nos cálices e nos frutos com sementes do hibisco foi determinado. A atividade antimicrobiana do extrato alcoólico de cálices, em ambos os acessos, apresentou diferença positiva significativa quando relacionada ao extrato alcoólico dos frutos com sementes. Salmonella Enteritidis foi a bactéria mais sensível ao extrato alcoólico de cálices do hibisco enquanto a mais resistente foi Staphylococcus aureus, independente da variável acesso, considerando somente a estrutura vegetal. Em relação ao extrato alcoólico dos frutos com sementes, Escherichia coli demonstrou a maior sensibilidade e Staphylococcus aureus a maior resistência. Os valores de polifenóis totais e de antocianinas do extrato alcoólico de cálices apresentaram diferença significativa entre si e foram superiores ao extrato alcoólico dos frutos com sementes. Possivelmente existe uma relação direta entre a concentração de antocianina e a atividade antibacteriana em diferentes estruturas vegetais do hibisco.The hibiscus (Hibiscus sabdariffa L.) has antioxidant and antimicrobial properties and it is utilized as functional food and medicinal plant in tropical countries. Through of Dilution Testing in Multiple Tubes System, it were determined the intensity of bacterial inhibition activity (IINIB/ Bacteriostasy) and the intensity of bacterial inactivation activity (IINAB/ Bactericidie) of alcoholic extracts of two accesses of hibiscus, known as: Palmares do Sul/RS and Porto Alegre/RS on standardized inoculum of Enterococcus faecalis (ATCC 19433), Escherichia coli (ATCC 11229), Salmonella Enteritidis (ATCC 11076) and Staphylococcus aureus (ATCC 25923). At the same time, the total content of polyphenols and anthocyanins in the calyxes and fruits with seeds hibiscus was determined. The antimicrobial activity of alcoholic extract of the calyxes in both accesses showed a significant positive difference when related to the alcoholic extract of the fruits with seeds. Salmonella enteritidis was the most sensitive bacteria to the alcoholic extract of calyxes of the hibiscus while the most resistant was Staphylococcus aureus, independent of the variable access, considering only the plant structure. In relation to the alcoholic extract of the fruits with seeds, Escherichia coli showed the highest sensitivity and Staphylococcus aureus the highest resistence. Total polyphenols and anthocyanins of alcoholic extract of calyxes‟s values showed a significant difference and they were superior to alcohol extract of fruits with seeds. Possibly there is a direct relationship between the concentration of anthocyanin and antibacterial activity in different structures of the hibiscus plant.
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Piovesana, Alessandra. « Extração, identificação, quantificação e microencapsulamento por atomização e liofilização de compostos bioativos dos cálices de hibisco (hibiscus sabdariffa l.) ». reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2016. http://hdl.handle.net/10183/151290.
Texte intégralRésumé :
O interesse pela extração dos compostos bioativos, a partir de fontes naturais, para o emprego na produção de alimentos funcionais tem aumentado, devido, principalmente, à crescente demanda por parte dos consumidores, por produtos mais saudáveis e que possam trazer benefícios à saúde. Dentre as fontes naturais de compostos bioativos, destaca-se o hibisco (Hibiscus sabdariffa L.), que é rico em antocianinas, flavonoides, ácidos fenólicos, carotenoides, dentre outros. Entretanto, quando os compostos bioativos são separados de suas matrizes, estes se tornam altamente instáveis frente a diversos fatores ambientais, necessitando serem protegidos. O recobrimento por microencapsulamento é uma alternativa para retardar a degradação desses compostos. Este estudo teve como objetivo a extração, identificação, quantificação e microencapsulamento por atomização e liofilização dos compostos bioativos dos cálices do hibisco. Primeiramente, foi realizada a extração exaustiva total dos carotenoides e compostos fenólicos por meio de solventes orgânicos, para a identificação e quantificação desses compostos. Também foi estudada a extração de antocianinas e demais compostos fenólicos por meio de solvente aquoso acidificado (ácido cítrico 2 %, p/v). A partir do melhor tratamento de extração, o extrato obtido foi microencapsulado mediante atomização e liofilização, empregando goma arábica (GA), goma guar parcialmente hidrolisada (GGPH) e polidextrose (PD) como agentes encapsulantes, na concentração de 10%. Os carotenoides e compostos fenólicos foram identificados e quantificados por HPLC-DAD-MS/MS (cromatografia líquida de alta eficiência com detecção por arranjo de diodos e espectrometria de massa). Vinte e um carotenoides foram encontrados, dos quais, quinze foram identificados. O total de carotenoides nos cálices de hibisco foi de 641,38 ± 23,61 μg/100 g massa fresca, sendo a all-trans-luteína e o all-trans--caroteno os compostos majoritários, representando 49 e 23%, respectivamente. Para os compostos fenólicos, foram encontrados vinte compostos, dos quais, catorze foram identificados. As antocianinas foram os compostos majoritários nos cálices de hibisco, sendo que a delfinidina 3-sambubiosídeo e cianidina 3-sambubiosídeo representaram 41 e 13% do total de compostos fenólicos, respectivamente. Dentre os ácidos fenólicos, os componentes majoritários foram o ácido 3-cafeoilquínico e ácido 5-cafeoilquínico, representando 15 e 13% do total de compostos fenólicos, respectivamente. Para a extração aquosa acidificada, foi utilizado um planejamento experimental fatorial fracionado (24-1), com quatro fatores: concentração de enzima, temperatura, velocidade de agitação e tempo de extração. A partir da ANOVA, os efeitos principais e de interação foram avaliados, tendo como respostas Chroma, antocianinas monoméricas totais (TMA), capacidade redutora, ABTS e compostos fenólicos. A partir dos resultados, o melhor tratamento foi: 55 °C, 50 μL de enzima/1000 g extrato, 400 rpm e 4 horas de extração, obtendo-se nessa condição de extração 3,82 mg/g extrato em base seca para TMA e 17,59 mg/g de extrato em base seca para compostos fenólicos totais, que resultou em capacidade antioxidante de 7,72 μmol Eq. Trolox/g de extrato em base seca, avaliado por ABTS e de 3,96 mg GAE/g de de extrato em base seca, avaliado pela capacidade redutora. Este extrato foi empregado no estudo de encapsulamento, por atomização (140 ºC) e liofilização (-68 ºC por 24 horas), utilizando GA, GGPH e PD como encapsulantes. Observou-se que o melhor tratamento foi por liofilização empregando GA como encapsulante, resultando em 2,83 mg/g amostra em base seca para TMA, capacidade antioxidante de 2,98 mg GAE/g amostra em base seca e 5.67 μmol Eq. Trolox/g amostra em base seca, avaliados por capacidade redutora e ABTS, respectivamente. Entretanto, quando foram avaliadas as propriedades físicas e morfológicas dos pós, as amostras elaboradas por atomização e usando GA e GGPH apresentaram os melhores desempenhos, onde os valores de solubilidade, higroscopicidade e umidade foram de 95,8 e 95,2%, 31,3 e 28,9%, 1,9 e 2,4%, respectivamente. Para a temperatura de transição vítrea (Tg), os tratamentos que utilizaram GA e GGPH nos dois métodos de encapsulamento, tiveram os maiores valores de Tg, variando de 10,9 a 17,4 ºC. Já para os tratamentos que utilizaram a PD como material de parede, os valores foram de (0,7 °C), tanto na atomização como na liofilização. Na microscopia também foi observado um melhor desempenho nas micropartículas atomizadas usando GA e GGPH, as quais mostraram partículas mais esféricas e sem tendência de atração e aderência entre si. Em relação ao diâmetro médio de partícula (D[4, 3]), os tratamentos liofilizados tiveram partículas maiores que os atomizados, variando de 101,7 a 143,1 μm para os liofilizados, e de 5,4 a 7,3 μm para os atomizados. Quanto ao span, o qual avalia distribuição de tamanho de partícula, variou de 1,90 a 2,00 para as amostras atomizadas e de 3,06 a 3,19 para as amostras liofilizadas, indicando que houve uma boa uniformidade na distribuição de tamanho de partícula. Conclui-se que o hibisco é uma matriz com ampla composição de compostos bioativos e tem potencial para aplicação em alimentos.The interest in the extraction of bioactive compounds from natural sources, for use in the production of functional foods has increased, mainly due to the growing demand by consumers for healthier products and can bring health benefits. Among the natural sources of bioactive compounds, stands out the hibiscus (Hibiscus sabdariffa L.), which is rich in anthocyanins, flavonoids, phenolic acids, carotenoids, among others. However, when the bioactive compounds are separated from their matrix, they become highly unstable against various environmental factors and need to be protected. The coating by microencapsulation is an alternative to slow the degradation of these compounds. This study aimed at the extraction, identification, quantification and microencapsulation by spray drying and freeze drying of bioactive compounds of hibiscus calyces. Firstly, a thorough exhaustive extraction of carotenoids and phenolic compounds by organic solvents was performed for identification and quantification of these compounds. The extraction of anthocyanins was also studied along with other phenolic compounds by an aqueous solvent acidified (2% citric acid, w/v). From the best treatment for extraction, the extract obtained was microencapsulated by spray drying and freeze drying using Arabic gum (GA), partially hydrolyzed guar gum (PHGG) and polydextrose (PD) as encapsulating agents in a concentration of 10%. Carotenoids and phenolic compounds were identified and quantified by HPLC-DAD-MS/MS (high-performance liquid chromatography with diode array detection and mass spectrometry). Twenty-one carotenoids were found, of which fifteen were identified. The total carotenoids in hibiscus calyces was 641.38 ± 23.61 mg/100 g fresh weight, with the all-trans-lutein and all-trans-β-carotene the major compounds, representing 49 and 23%, respectively. Regarding the phenolic compounds it was found twenty of those, of which fourteen have been identified. Anthocyanins were the main components in the hibiscus calyces, and delphinidin and cyanidin 3-sambubioside 3-sambubioside represented 41 and 13% of total phenolic compounds, respectively. Among the phenolic acids, the major components were the 3-caffeoylquinic acid and 5-caffeoylquinic acid, representing 15 and 13% of total phenolic compounds, respectively. For acidified aqueous extraction, we used a fractional factorial design (24-1) with four factors: enzyme concentration, temperature, stirring speed and extraction time. From the ANOVA, the main and interaction effects were assessed as answers: Chroma, total anthocyanins monomeric (TMA), reducing capacity, ABTS and phenolic compounds. From the results, the best treatment was with 55 °C, 50 μL of enzyme/1000 g extract, 400 rpm and 4 hours of extraction, it was obtained in this extraction condition 3.82 mg/g extract on a dry basis for TMA and 17.59 mg/g extract on a dry basis for phenolic compounds, which resulted in antioxidant capacity of 7.72 μmol Eq. Trolox/g extract on a dry basis, evaluated by ABTS and 3.96 mg GAE/g extract on a dry basis, assessed by reducing capacity. This extract was used for the encapsulation study, by spray drying (140 °C) and freeze drying (-68 ° C for 24 hours) using GA, PHGG, and PD as encapsulants. It was observed that the best treatment is by freeze drying using GA as encapsulant, resulting in 2.83 mg/g sample on dry basis for TMA, antioxidant capacity of 2.98 mg GAE/g sample on dry basis and 5.67 μmol Eq. Trolox/g sample on dry basis, evaluated by reducing capacity and ABTS, respectively. However, when we evaluated the physical and morphological properties of powders, samples prepared by spray drying and using GA and PHGG showed the best performance, and the values for solubility, hygroscopicity and moisture were 95.8 and 95.2%, 31.3 and 28.9%, 1.9 and 2.4%, respectively. For the glass transition temperature (Tg), treatments with GA and PHGG on both encapsulation methods had high Tg values ranging from 10.9 to 17.4 °C. As for treatments of PD as wall material, the values were (0.7 °C), both the spray drying as in freeze drying. In microscopy was also observed improved performance in spray-dried microparticles using GA and PHGG, which showed more spherical particles and with no tendency to attract and adhere to each other. Regarding the average particle diameter (D [4, 3]), the freeze-dried treatments had higher spray-dried particles ranging from 101.7 to 143.1 μm for freeze-dried, and 5.4 to 7.3 μm for spray-dried. As the span, which assesses particle size distribution ranged from 1.90 to 2.00 for spray-dried samples and 3.06 to 3.19 for the freeze-dried samples, indicating that there was a good uniformity in the size in the distribution of the size of the particle. It follows that hibiscus is a matrix with broad composition and bioactive compounds have potential for application in foods.
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Bussalleu, Vargas Quintanilla Vanessa, Cabrera José Julián Estabridis, Villanueva Sandra Judith Levano et Olcese Jose Alberto Osterling. « Hibiscus ». Bachelor's thesis, Universidad Peruana de Ciencias Aplicadas (UPC), 2020. http://hdl.handle.net/10757/653373.
Texte intégralRésumé :
El presente trabajo de investigación trata de la producción mediante servicio de maquila y la comercialización de tés de 45 gr. con la marca Hibiscus que es una mezcla de hojas deshidratadas de planta aromáticas, medicinales que mantienen sus nutrientes y propiedades funcionales, tales como: digestivo, relajante y antioxidante como una solución para mejorar la salud y bienestar del consumidor. Además, para simplificar el proceso de preparación del filtrado del té, la presentación del producto trae un infusor de té para taza.
En este sentido, para posicionar el producto en el consumidor, se buscará que la experiencia del consumidor relacione el producto con la salud en el segmento de varones y mujeres de 25 a 55 años de edad del nivel socioeconómico A y B que residen en Lima Moderna de Lima Metropolitana, con estilo de vida sofisticado y moderno, que cuidan su imagen personal y prefieren productos saludables que les facilite ahorro de tiempo en la preparación. Cabe indicar, el negocio Hibiscus tiene potencial crecimiento por la tendencia de la alimentación saludable, el crecimiento promedio de la venta anual es 4%.
Hibiscus Té será distribuido por el canal directo a través de módulo de venta en Jockey Plaza y Marketplace de Facebook y por el canal indirecto en tiendas saludables como son: Sanahoria, Flora y Fauna, Ecotienda, y plataformas webs: ecotienda.pe, limaorganica.pe y mercadillo.pe. también, se contará con delivery para la entrega del pedido en 24 horas.He following business research paper deals with the production process, marketing and sales of 45 gr. packed teas under the brand ‘Hibiscus’. The teas contain a mixture of dehydrated, aromatic, medicinal plant leafs that maintain their nutrients and functional properties, such as: digestive, relaxing and antioxidant. They serve as a solution to improve consumer health and well-being. In addition, to simplify the process of preparing the tea (filtering), the product presentation comes with a tea infuser. In this sense, to adequately position the product in the market, we must provide a great consumer experience, making our customers relate the product with good health. Our range of consumers belong to the segment of 25 to 55 year old men & women, of the socioeconomic levels A and B, who reside in the metropolitan, modern area of Lima. They have a sophisticated and modern lifestyle, take care of their personal image and prefer healthy products that facilitate saving time in preparation. It should be noted that the Hibiscus business has good potential growth due to the trend of healthy eating. The average growth of annual sales is projected at 4%. Hibiscus Tea will be distributed through a direct channel by the sales module in Jockey Plaza, via Facebook Marketplace, and through the indirect channel in a variety of healthy stores. These include: Sanahoria, Flora and Fauna, Ecotienda, and web platforms such as ecotienda.pe, limaorganica.pe and mercadillo.pe. Finally, there will be a delivery service for orders made during a 24 hours scale.
Trabajo de investigación
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Paul, Hélène. « "Hibiscus sabdariffa" L ». Paris 5, 1995. http://www.theses.fr/1995PA05P111.
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Sindi, Hebah Abbas. « Bioactivity of anthocyanins from Hibiscus sabdariffa ». Thesis, University of Leeds, 2013. http://etheses.whiterose.ac.uk/5910/.
Texte intégralRésumé :
A systematic study on extraction of Hibiscus sabdariffa was carried out for the first time using different solvents (water, methanol, ethyl acetate and hexane) in the presence and absence of formic acid, using different extraction times and temperatures. The extracts were analysed for total phenol content, antioxidant capacity using DPPH, FRAP and TEAC assays, and total monomeric anthocyanin content. In addition, specific anthocyanins were determined using HPLC and LC-MS. The results showed the highest antioxidant capacities were obtained by extracting using water, with or without formic acid, for 10 min at 100 °C. These extracts provided the highest concentrations of cyanidin 3-sambubioside and delphinidin 3-sambubioside. Commercially available herbal teas containing H. sabdariffa were analysed. The study found that contents of total phenols, anthocyanins and antioxidant capacity were higher when using the optimal extraction procedure, suggesting that putative health benefits could be increased by altering processing methods. The partition coefficients (log p) of anthocyanins found in H. sabdariffa, were measured showed that aglycone and glucoside forms of hibiscus anthocyanins behave differently when in the presence of cell wall material. Such behaviour could, in vivo, affect the absorption and bioactivity of these anthocyanins, and therefore, their efficacy. A human crossover study investigated the effect of daily consumption for 8 weeks of a H. sabdariffa juice for 8 weeks on the blood pressure of healthy subjects (n= 29). Cranberry juice was used as the control. A significant reduction was found in systolic blood pressure (but not diastolic) compared to the baseline. No significant effect on blood pressure was seen with cranberry juice. The study suggests that regular consumption of extracts of H. sabdariffa may reduce the risk of cardiovascular disease, on the other hand people with low blood pressure should consumed it very carefully due to hypotensive effect of the extract.
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Borba, Juliana Becker. « O efeito da infusão das flores de Hibiscus pernambucensis Arruda sobre ratas gestantes e lactantes expostas ao campo eletromagnético de ultra-alta-frequência ». reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2010. http://hdl.handle.net/10183/21409.
Texte intégralRésumé :
O Hibiscus pernambucensis Arruda (HpA) é uma planta da família Malvaceae, típica de clima tropical de ecossistemas de mangues. A infusão das flores do HpA apresenta propriedades antioxidantes, decorrentes da vitamina E, flavonóides, quercetina e taninos. Há estudos mostrando que os campos eletromagnéticos de ultra-alta-freqüência (CEMUAF) podem provocar danos à saúde. Estes danos acontecem por indução de estresse oxidativo às células. O presente estudo visa verificar o efeito antioxidante das flores de HpA sobre o fígado e o córtex cerebral de ratas expostas aos CEMUAF durante a gestação e a lactação. Foram utilizadas 24 ratas fêmeas Wistar de 3 meses de idade que foram divididas em 4 grupos: 6 ratas controles (RC), 6 ratas expostas (RE), 6 ratas controles (RCH) e 6 ratas expostas (REH) que tomaram a infusão das flores de HpA. Foi administrada diariamente 1,5ml da infusão, em uma concentração de 5%, 30 min antes da exposição, durante 42 dias. As REH foram irradiadas 30 min por dia, por toda gestação e lactação, em uma freqüência de 950MHz, 1W de potência, campo elétrico médio de 35 V/m e antena de polarização vertical. A taxa de absorção específica do grupo exposto variou de 0,64 a 0,44W/kg no cérebro e de 0,99 a 0,67W/kg no fígado, estes valores foram calculados para ratas variando de 90 a 132 dias de idade. Após este tempo, as ratas foram decapitadas, o fígado e córtex cerebral foram retirados e imersos no nitrogênio líquido para posterior análise. Os resultados mostraram que a exposição ao CEMUAF e a infusão de HpA causaram um aumento significativo do dano oxidativo em lipídios e DNA no córtex cerebral esquerdo (CCE) (p<0,05). Nossos resultados indicam que o CEMUAF de 950MHz e a infusão de HpA provocam estresse oxidativo e é genotóxico ao CCE de RE e RCH. Quando combinado CEMUAF e a infusão, houve diminuição dos danos em DNA no REH. Nas análises quantitativas de conteúdo de polifenol total e flavonóides, observou-se que a infusão de HpA contém uma grande quantidade de polifenóis totais mas uma pequena de flavonóides (quando comparado ao H.sabdariffa) o que não foram capazes de diminuir os danos oxidativos nos animais.The Hibiscus pernambucensis Arruda (HpA) is a plant of the Malvaceae family, typical of tropical mangrove ecosystems. The infusion of the flowers of the HpA has antioxidant properties, resulting from vitamin E, flavonoids, quercetin and tannins. There are studies showing that electromagnetic fields of ultra-high frequency (UHF-EMF) can cause damage to health. These damages occur by induction of oxidative stress to cells. This study aims to determine the antioxidant effect of the flowers of HpA on the liver and cerebral cortex of rats exposed to UHF-EMF during pregnancy and lactation. Twenty-four female Wistar rats of average age three months were distributed into four groups: 6 control rats (CR), 6 exposed rats (ER), 6 control rats (HCR) and 6 exposed rats (HER). The animal recived on daily basis an amount of 1,5 ml of the infusion of flowers HpA, at a concentration of 5%, 30min before exposure, for 42 days. The ER were irradiated 30 minutes per day throughout pregnancy and lactation, at a frequency of 950MHz, 1W of power, the average electric field of 35V/m and antenna for vertical polarization. The specific absorption rate of the exposed group ranged from 0.64 to 0.44W/kg in the brain and from 0.99 to 0.67W/kg in the liver. These values were calculated for rats ranging from 90 to 132 days of age. After this time, the rats were decapitated, the liver and cerebral cortex were removed and immersed in liquid nitrogen for later analysis. The results are presented as mean ± SEM. The values were assessed by post-ANOVA SNK (two tails), with the significant results p<0,05 for n=6. The results showed that exposure to UHF-EMF and the infusion of HpA caused a significant increase in oxidative damage in lipids and DNA in the left cerebral cortex (LCC) (p <0,05). The results indicate that UHF-EMF of 950MHz and the infusion of HpA cause oxidative stress and is genotoxic to LCC ER and HCR. When combined UHFEMF and infusion decreased DNA damage in the HER. Quantitative analysis of total polyphenol content and flavonoids showed that the infusion of HpA contains a large amount of total polyphenols but little of flavonoids (compared to H.sabdariffa) which have not been able to reduce oxidative damage in animals.
Livres sur le sujet "Hibiscu"
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Reardon, Richard C. Pink hibiscus mealybug, Maconellicoccus hirsutus (Green) = : La cochinilla rosada del hibisco, Maconellicoccus hirsutus (Green). Washington, D.C.?] : U.S. Dept. of Agriculture, Animal and Plant Health Inspection Service, 1998.
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name, No. Have you seen the pink hibiscus mealybug ? = : Ha visto usted la cochinilla rosada del hibisco ? Rio Piedras, P.R : U.S. Dept. of Agriculture, International Institute of Tropical Forestry, 1998.
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Walker, Jacqueline. Hibiscus. Buffalo, N.Y : Firefly Books, 2001.
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Morris, Paula. Hibiscus Coast. Auckland, N.Z : Penguin, 2005.
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Ku, Hua. Hibiscus : Roman. Paris : R. Laffont, 1987.
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Adichie, Chimamanda Ngozi. Paarse hibiscus. Amsterdam : Muntinga Pockets, 2005.
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Jim, Howie, dir. Growing hibiscus. [Kenthurst, NSW] : Kangaroo Press, 1985.
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Adichie, Chimamanda Ngozi. Purple Hibiscus. New York : Anchor Books, 2004.
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Roché, Cindy Talbott. Venice mallow (Hibiscus trionum L.). [Olympia, Wash.] : Washington State University Cooperative Extension, 1992.
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Grover, Robin. Why the hibiscus ? : Place names of the Hibiscus Coast. Orewa [N.Z.] : Silverdale & Districts Historical Society, 2008.
Trouver le texte intégralChapitres de livres sur le sujet "Hibiscu"
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Ross, Ivan A. « Hibiscus sabdariffa ». Dans Medicinal Plants of the World, 267–75. Totowa, NJ : Humana Press, 2003. http://dx.doi.org/10.1007/978-1-59259-365-1_13.
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Lim, T. K. « Hibiscus mutabilis ». Dans Edible Medicinal and Non Medicinal Plants, 300–305. Dordrecht : Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-8748-2_21.
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Lim, T. K. « Hibiscus sabdariffa ». Dans Edible Medicinal and Non Medicinal Plants, 324–70. Dordrecht : Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-8748-2_23.
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Lim, T. K. « Hibiscus schizopetalus ». Dans Edible Medicinal and Non Medicinal Plants, 371–73. Dordrecht : Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-8748-2_24.
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Lim, T. K. « Hibiscus syriacus ». Dans Edible Medicinal and Non Medicinal Plants, 374–80. Dordrecht : Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-8748-2_25.
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Lim, T. K. « Hibiscus taiwanensis ». Dans Edible Medicinal and Non Medicinal Plants, 381–84. Dordrecht : Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-8748-2_26.
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Lim, T. K. « Hibiscus tiliaceus ». Dans Edible Medicinal and Non Medicinal Plants, 385–94. Dordrecht : Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-8748-2_27.
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Azimova, Shakhnoza S., et Anna I. Glushenkova. « Hibiscus furcatus ». Dans Lipids, Lipophilic Components and Essential Oils from Plant Sources, 620–21. London : Springer London, 2012. http://dx.doi.org/10.1007/978-0-85729-323-7_2019.
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Azimova, Shakhnoza S., et Anna I. Glushenkova. « Hibiscus grandiflorus ». Dans Lipids, Lipophilic Components and Essential Oils from Plant Sources, 621. London : Springer London, 2012. http://dx.doi.org/10.1007/978-0-85729-323-7_2020.
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Azimova, Shakhnoza S., et Anna I. Glushenkova. « Hibiscus inebrum ». Dans Lipids, Lipophilic Components and Essential Oils from Plant Sources, 621. London : Springer London, 2012. http://dx.doi.org/10.1007/978-0-85729-323-7_2021.
Texte intégralActes de conférences sur le sujet "Hibiscu"
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Brito, Isabelle, Jayme César, Bruno Raniere Lins de Albuquerque Meireles, Kataryne Árabe Rimá de Oliveira et Angela Maria Tribuzy de Magalhães Cordeiro. « AVALIAÇÃO DO POTENCIAL ANTIOXIDANTE E IDENTIFICAÇÃO DE COMPOSTOS FENÓLICOS DE DIFERENTES EXTRATOS DE HIBISCO (Hibiscus sabdariffa L.) ». Dans V ENCONTRO NACIONAL DA AGROINDúSTRIA. Galoa, 2019. http://dx.doi.org/10.17648/enag-2019-115115.
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Somera, Tânia, Janiele Dos Santos et Tahis Regina Baú. « EFEITO DAS CONDIÇÕES DE INFUSÃO SOBRE O TEOR DE COMPOSTOS BIOATIVOS DO CHÁ DE HIBISCO (Hibiscus sabdariffa L.) ». Dans Anais do I Simpósio Online Sulamericano de Tecnologia, Engenharia e Ciência de Alimentos. Recife, Brasil : Even3, 2022. http://dx.doi.org/10.29327/161828.1-36.
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Priya, Lakshmi, et Arulmozhi A. « Bio coating as an alternate for wax coating for food grade paper boards ». Dans 11th International Symposium on Graphic Engineering and Design. University of Novi Sad, Faculty of technical sciences, Department of graphic engineering and design, 2022. http://dx.doi.org/10.24867/grid-2022-p7.
Texte intégralRésumé :
1.45 million tons of paraffin wax-coated boxes of used products enter landfills every year, and 4.5 million metric tons of carbon dioxide is released during the recycling process. Therefore, the goal of the research is to find out an alternative to the synthetic wax coating seen on food product cartons. This research uses natural coating materials that are more effective than synthetic coating materials, such as hibiscus, gum Arabic, and turmeric. The natural antibacterial herb turmeric's curcumin is extracted using ethonal, and then heating is used to create the hibiscus extract. Three layers are coated on the package: the first layer is curcumin, which serves as the package's antimicrobial coating; the second layer is gum Arabic, which serves as a binder to hold the third layer of hibiscus. These two layers serve as a barrier on the packaging, and then the hibiscus extract gel is applied over them. Using a spray gun, these three coats are applied to the packaging. The method used to dry the coatings is air or sun drying. The test findings are assessed for the burst strength, burst factor, moisture content, smoothness, calliper thickness, and micropsoic analyses of the wax coated and bio coated carton samples.
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Santos, U. V., B. S. Santos, G. F. Silva, P. B. L. Constant et J. A. B. Santos. « AVALIAÇÃO DE POTENCIAL DE ERVAS MEDICINAIS : CAPIM-LIMÃO (Cymbopogon citratus D.C.), CHÁ VERDE (Camellia sinensis L.) E HIBISCO (Hibiscus sabdariffa L.) PARA OBTENÇÃO DE CHÁS SOLÚVEIS ». Dans 5th International Symposium on Technological Innovation. Universidade Federal do Sergipe, 2014. http://dx.doi.org/10.7198/s2318-3403201400020044.
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da Silva, Elen, Edson Pontes, Maria Alves, Maria Souza, Camila Nascimento, Claudiele Dantas, Thalia da Costa et Edna Silva. « Utilização do Hibisco para Emagrecimento ». Dans XXI I Congresso Brasileiro de Nutrologia. Thieme Revinter Publicações Ltda, 2018. http://dx.doi.org/10.1055/s-0038-1675102.
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Ramelan, A. H., S. Wahyuningsih, N. A. Rosyida, E. Supriyanto, S. Saputro, Q. A. Hanif et L. Rinawati. « Anthocyanin extracted from Hibiscus (Hibiscus rosa sinensis L.) as a photosensitizer on nanostructured-TiO2 dye sensitized solar cells ». Dans PROCEEDINGS OF INTERNATIONAL SEMINAR ON MATHEMATICS, SCIENCE, AND COMPUTER SCIENCE EDUCATION (MSCEIS 2015). AIP Publishing LLC, 2016. http://dx.doi.org/10.1063/1.4941478.
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Rachma, I., A. Dwiranti et A. Salamah. « Optimization of Hibiscus rosa-sinensis L. chromosome pretreatment ». Dans PROCEEDINGS OF THE 3RD INTERNATIONAL SYMPOSIUM ON CURRENT PROGRESS IN MATHEMATICS AND SCIENCES 2017 (ISCPMS2017). Author(s), 2018. http://dx.doi.org/10.1063/1.5064146.
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Yani, Nadia Falihah Ahmad, Nurhazwani Ismail et Kai Siang Oh. « Potential of using Hibiscus Sabdariffa in treating greywater ». Dans PROCEEDINGS OF THE INTERNATIONAL ENGINEERING RESEARCH CONFERENCE - 12TH EURECA 2019. AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5120993.
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Rithichai, P., Y. Jirakiattikul, C. Sriboonthai et A. Itharat. « Bioactive Compounds and Antioxidant in Hibiscus sabdariffa Leaf ». Dans GA 2017 – Book of Abstracts. Georg Thieme Verlag KG, 2017. http://dx.doi.org/10.1055/s-0037-1608056.
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Vinícius Falci et Caroline Maria Calliari. « Elaboração e caracterização de licor de Hibiscus sabdariffa ». Dans XX Seminário de Iniciação Científica e Tecnológica da UTFPR. Curitiba, PR, Brasil : Universidade Tecnológica Federal do Paraná - UTFPR, 2015. http://dx.doi.org/10.20906/cps/sicite2015-0459.
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