Bibliographies thématiques / Hemagglutination Assay

Littérature scientifique sur le sujet « Hemagglutination Assay »

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Publié le 6 septembre 2023

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Articles de revues sur le sujet "Hemagglutination Assay"

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Enders, Martin, Uwe Bartelt, Frank Knotek, Kristina Bunn, Sirpa Strobel, Klaus Dietz et Gisela Enders. « Performance of the Elecsys Rubella IgG Assay in the Diagnostic Laboratory Setting for Assessment of Immune Status ». Clinical and Vaccine Immunology 20, no 3 (23 janvier 2013) : 420–26. http://dx.doi.org/10.1128/cvi.00688-12.

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ABSTRACTRubella in early pregnancy bears a high risk for congenital defects (e.g., cataracts, hearing loss, and heart disease) and for long-term sequelae in the newborn. Despite implementation of vaccination programs in many regions, the threat of devastating consequences from congenital rubella virus infection remains and careful screening of maternal immune status before and during pregnancy helps to reduce the risk. This study compared the performance of the Elecsys Rubella IgG assay with that of other assays routinely used for screening. Samples from 1,090 women undergoing routine antenatal care were tested using the Elecsys and Enzygnost Rubella IgG assays and the hemagglutination inhibition test. Samples with hemagglutination inhibition titers of <32 (n= 148) were additionally tested using the Vidas, AxSYM, Liaison, and Architect Rubella IgG assays. Agreement of qualitative results from the Elecsys, Enzygnost, and hemagglutination inhibition assays was good in all samples. All assays showed 100.0% specificity. In samples with hemagglutination inhibition titers of <32, the Elecsys, AxSYM, and Enzygnost assays showed higher sensitivity (>90.0%) than the other immunoassays (78.6 to 82.4%). The Elecsys assay reported significantly higher rubella virus IgG levels than the other immunoassays across the whole set of 1,090 samples, with the largest bias and deviation from limits of agreement in Bland-Altman analysis. In conclusion, the Elecsys assay is highly sensitive and specific with regard to qualitative results and suitable for routine automated screening. However, given the considerable variation between quantitative results from different immunoassays, testing methods should be documented and the same assay used throughout an individual's antenatal follow-up wherever possible.
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Bǎlunǎ, Roxana-Georgeta, Doina Barac, Ruxandra Tarnavschi et Irena Belaşcu. « Hemagglutination assay for human serum fibronectin ». Journal of Immunological Methods 79, no 1 (mai 1985) : 65–70. http://dx.doi.org/10.1016/0022-1759(85)90392-8.

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Nam, Sung-Wook, Dong-Gyu Jeon, Young-Ran Yoon, Gang Ho Lee, Yongmin Chang et Dong Il Won. « Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips ». Biosensors 13, no 7 (14 juillet 2023) : 733. http://dx.doi.org/10.3390/bios13070733.

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Hemagglutination assay has been used for blood typing and detecting viruses, thus applicable for the diagnosis of infectious diseases, including COVID-19. Therefore, the development of microfluidic devices for fast detection of hemagglutination is on-demand for point-of-care diagnosis. Here, we present a way to detect hemagglutination in 3D microfluidic devices via optical absorbance (optical density, OD) characterization. 3D printing is a powerful way to build microfluidic structures for diagnostic devices. However, mixing liquid in microfluidic chips is difficult due to laminar flow, which hampers practical applications such as antigen-antibody mixing. To overcome the issue, we fabricated 3D microfluidic chips with embedded microchannel and microwell structures to induce hemagglutination between red blood cells (RBCs) and antibodies. We named it a 3D microtrap chip. We also established an automated measurement system which is an integral part of diagnostic devices. To do this, we developed a novel way to identify RBC agglutination and non-agglutination via the OD difference. By adapting a 3D-printed aperture to the microtrap chip, we obtained a pure absorbance signal from the microchannels by eliminating the background brightness of the microtrap chip. By investigating the underlying optical physics, we provide a 3D device platform for detecting hemagglutination.
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Patel, C. P., et T. W. Willis. « Potato lectin activity assay based on hemagglutination ». Journal of the Science of Food and Agriculture 60, no 1 (1992) : 113–19. http://dx.doi.org/10.1002/jsfa.2740600118.

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Nguyen, Michael, Katherine Fries, Rawia Khoury, Lingyi Zheng, Branda Hu, Stephen W. Hildreth, Robert Parkhill et William Warren. « Automated Imaging and Analysis of the Hemagglutination Inhibition Assay ». Journal of Laboratory Automation 21, no 2 (avril 2016) : 287–96. http://dx.doi.org/10.1177/2211068215610061.

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Chantratita, Narisara, Vanaporn Wuthiekanun, Aunchalee Thanwisai, Direk Limmathurotsakul, Allen C. Cheng, Wirongrong Chierakul, Nicholas P. J. Day et Sharon J. Peacock. « Accuracy of Enzyme-Linked Immunosorbent Assay Using Crude and Purified Antigens for Serodiagnosis of Melioidosis ». Clinical and Vaccine Immunology 14, no 1 (8 novembre 2006) : 110–13. http://dx.doi.org/10.1128/cvi.00289-06.

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ABSTRACT Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).
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Noah, Diana L., Heather Hill, David Hines, E. Lucile White et Mark C. Wolff. « Qualification of the Hemagglutination Inhibition Assay in Support of Pandemic Influenza Vaccine Licensure ». Clinical and Vaccine Immunology 16, no 4 (18 février 2009) : 558–66. http://dx.doi.org/10.1128/cvi.00368-08.

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ABSTRACT Continued outbreaks of highly pathogenic avian influenza over the past decade have spurred global efforts to develop antivirals and vaccines. As part of vaccine development, standard methods are needed for determining serum antibody titers in response to vaccination. Hemagglutination inhibition (HAI) assays are appropriate for assessing the immunogenicity of pandemic influenza vaccines in support of license approval. We demonstrate that a rigorous qualification of the HAI assay for H5N1 influenza virus, evaluating for precision, intermediate precision, linearity, range, specificity, and robustness, satisfies the intent of regulatory guidance for assay validation despite the lack of availability of specific reference standard antigens and antisera.
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Wang, Xiaolei, Zhiyuan Yang, Xiuqing Wang, Huijuan Duan, Lixin Liu, Huimin Cheng, Chenghuai Yang et al. « Development of a Hemagglutination Inhibition Assay for Duck Tembusu Virus ». Avian Diseases 63, no 2 (18 janvier 2019) : 298. http://dx.doi.org/10.1637/11954-082018-reg.1.

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CHENG, ALLEN C., GARY LUM, KEVIN FREEMAN, BART J. CURRIE et MATHEW O’BRIEN. « INDIRECT HEMAGGLUTINATION ASSAY IN PATIENTS WITH MELIOIDOSIS IN NORTHERN AUSTRALIA ». American Journal of Tropical Medicine and Hygiene 74, no 2 (1 février 2006) : 330–34. http://dx.doi.org/10.4269/ajtmh.2006.74.330.

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Saha, Repon Kumer, Srijan Acharya, Maha Jamiruddin, Priyanka Roy, Md Sohidul Islam et Syed Sahidul Haque Shovon. « Antimicrobial effects of a crude plant lectin isolated from the stem of Tinospora tomentosa ». Journal of Phytopharmacology 3, no 1 (25 janvier 2014) : 44–51. http://dx.doi.org/10.31254/phyto.2014.3107.

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Crude plant lectins were isolated from the stem of Tinospora tomentosa and found its antibacterial and antifungal effects. Lectins were isolated by ammonium sulphate precipitation method. Presence of carbohydrate and proteins were investigated by thin layer chromatography and infrared spectroscopy techniques. Lectin was characterized by its binding affinity with carbohydrates and human erythrocytes by hemagglutination inhibition assay and SDS-page gel electrophoresis. The amount of proteins was quantitatively measured by Lowry method. Antibacterial and antifungal activities were investigated by disk diffusion assay. Minimum inhibitory concentrations of bacteria and fungus were determined from their dose-response curve. Salmonella induced hemagglutination activity was performed to investigate its binding affinity with bacterial cell surface. Isolated lectin contained carbohydrates and protein residues in its structure. Its molecular weight was about 32 kD and seemed as a monomeric. It showed binding affinities to lactose sugar and bacterial cell surfaces and inhibited hemagglutination. It showed a dose-response relationship in its antibacterial and antifungal activities. The stem of Tinospora tomentosa may be considered as an important medicinal plant for antimicrobial therapeutics.
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Thèses sur le sujet "Hemagglutination Assay"

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Ng, Hoi-yee Iris, et 吳凱怡. « Serological diagnosis of influenza B virus infection in pigs : a comparison of the hemagglutination inhibition assay and the cell-based ELISA assay ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/193797.

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Background Swine influenza virus (SIV) was first isolated in the United States in 1930 and was thereafter widely reported in many countries. Most SIVs that have been identified are influenza A viruses. There was no report of influenza B viruses isolated in swine. Seroepidemiological study in UK has shown a low seroprevalence of influenza B antibody in pigs. The primary serological test used to detect influenza antibody is the hemagglutination inhibition (HI)test. Enzyme-linked immunosorbent assay (ELISA) are also available commercially for detection of antibodies against influenza A viruses but not for the detection of influenza B antibodies. Objectives 1) To examine the prevalence of influenza B antibodies in pig sera sampled at the abattoir in Hong Kong. 2) To develop the cell-based ELISA assay for the detection of antibodies against influenza A and B viruses. 3) To compare the cell-based ELISA assays with three commercial ELISA kits, namely the IDVet ID Screen influenza A antibody competition ELISA, the IDEXX Influenza A Ab test and the IDEXX AI MultiS-Screen Ab test using swine sera. 4) To test swine sera using the influenza B cell-based ELISA assay to complement data on swine seroprevalence obtained with HI tests. Methods The first part of this study involved HI screening of 4643 pig sera from 2009 to 2012. These sera were tested for the presence of antibodies against B/Brisbane/60/2008 and B/Wisconsin/1/2010whichrepresent the B/Victoria and B/Yamagata lineages respectively. The second part of this study involved the development and performance evaluation of the cell-based ELISA assays. The cell-based ELISA assays were developed using influenza virus infected cells as the capture antigens and fluorescence-labelled anti-IgG antibody as the detection antibody. The viruses that were used to prepare the assays were A/California/04/2009, B/Brisbane/60/2008 and B/Wisconsin/1/2010. All three cell-based ELISA assays were tested with WHO reference sera and swine sera and the results were analyzed using paired t-test and receiver operating characteristic analysis. In addition, the results of the influenza A cell-based ELISA assay were compared with the commercial ELISA assay using Fisher’s exact two-tailed test, Pearson’s correlation analysis and Bland-Altman plot. Results A low prevalence (0.28%; 95%CI: 0.16%-0.47%) of influenza B antibody was observed inthe swine sera samples. The seroprevalence for B/Victoria was higher than that of B/Yamagatain 2010to2012. Co-existence of B/Victoria and B/Yamagata antibodies were found in the swine population during 2010 and 2011. The influenza A cell-based ELISA was found to have low sensitivity (64.1%;95%CI: 52.4%-74.4%) and high specificity (94.7%; 95%CI:80.9%-99.1%) when compared with the commercial ELISA assays. In contrast, using HI as the reference test influenza B cell-based ELISA prepared using B/Wisconsin/1/2010 infected cells were shown to have high sensitivity (92.31%; 95%CI:64.0%-99.8%) but low specificity (63.16%;95%CI:38.4%-83.7%) in detection of influenza B antibodies in swine sera. Conclusion Sporadic transmission of influenza B virus may occur in swine but there is no evidence for efficient and sustained transmission of the virus between them. Cell-based ELISA assay prepared with B/Wisconsin/1/2010 may be considered as an alternative screening testprior to HI subtyping.
published_or_final_version
Public Health
Master
Master of Public Health
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YANG, CHING-I., et 楊靜怡. « Functional Study of AcmJRL through Site-directed Mutagenesis by Hemagglutination Assay and QCM Analysis ». Thesis, 2019. http://ndltd.ncl.edu.tw/handle/2ahspe.

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Chen, Hui-Wen, et 陳慧文. « Sequence analyses, hemagglutination activity and development of diagnostic assays of avian infectious bronchitis viruses ». Thesis, 2010. http://ndltd.ncl.edu.tw/handle/02339965931162241536.

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博士
國立臺灣大學
獸醫學研究所
98
In the beginning one-third part of this dissertation (Unit 3 and 4), research has focused on the sequence analyses of IBVs isolates in Taiwan. The putative recombinant events of Taiwan IBVs isolated from 1992 to 2007 were investigated by phylogenetic analysis and simplot analysis. The 3’ 7.3 kb structural protein genome of eight Taiwan strains was directly sequenced. Frequent recombination events were identified among the Taiwan and China CK/CH/LDL/97I-type strains. Putative crossover sites were located in the S1, S2, 3b, M genes and the intergenic region between the M and 5a genes. All of the recombinants showed chimeric IBV genome arrangements originated from Taiwan and China-like parental strains. In addition, this study reports on a viral surveillance program in Taiwan from 2005 to 2006 with sampling conducted in poultry slaughter houses. Eight out of 47 flocks (17%) were IBV-infected, from which 13 IBV isolates were recovered. Eleven of 13 isolates (84.6%) clustered with Taiwan group I based on the S1 gene. One IBV isolate showed evidence of frequent recombination events with China-like IBVs in the S gene. Another isolate demonstrated the incorporation of China-like and H120-like genome fragments within the S2 gene and the M gene region, respectively. Some antigenic changes were found in the one-directional neutralization test. However, no positive selection pressures were related to those variations in the S1 genes among Taiwan IBVs. Field IBVs in Taiwan revealed intertypic genetic recombination and antigenic diversity. For the second one-third part of this dissertation (Unit 5 and 6), molecular and serological diagnosis of IBV were the research topics. Since a heterologous Mass-serotype vaccine has been used in Taiwan for a decade, group-specific identification on virus and antibody has been a difficult problem. The Unit 5 reports on a rapid and reliable multiplex reverse transcriptase-polymerase chain reaction (mRT-PCR) assay for the genotyping of IBVs. Local IBV strains and commercially available vaccines were used for evaluating the viral genotyping assay. A number of field isolates and were examined for clinical application. The results showed that all of the examined IBVs were accurately genotyped by identifying the corresponding bands on agarose gels. The mRT-PCR assay was able to detect as low as 103, 105 and 103 viral RNA copies of the TW-I, TW-II and Mass-type strains, respectively. The mRT-PCR assay accurately detected and differentiated vaccine viruses from wild-type strains in the field. Another aspect, in order to understand the status of field infection, a monoclonal antibody (mAb) blocking ELISA (b-ELISA) against local IBVs was developed. The selected mAb showed specificity to Taiwan IBV strains but no cross reactivity against the vaccine strain H120. By using the hemagglutination inhibition (HI) test as a gold standard, the cut-off value, sensitivity and specificity of the b-ELISA were evaluated with 390 field samples. The type-specificity of detection was validated with a panel of chicken hyperimmune sera. The results showed that the b-ELISA demonstrated high sensitivity (97.96%) and specificity (97.16%) of detection. The agreement between the results of b-ELISA and HI test was statistically significant (Kappa = 0.95) and no significant difference between these two methods (McNemar p = 0.72). The b-ELISA specifically detected Taiwan IBV serotypes rather than other three IBV serotypes and sera against other avian pathogens. This b-ELISA provides type-specific antibody detection to the local IBV strains. It has the potential to serve as a rapid and reliable diagnostic method of IBV clinical infections in the field of Taiwan. In the last one-third part of this dissertation (Unit 7 and 8), research started with the characterization of the hemagglutination activity (HA) in Taiwan IBV strains and ended up with discovering the cell-associated hemadsorption activity of baculovirus-derived S1 protein. The HA activity of 13 Taiwan IBV strains were investigated. The results showed 9 of 13 Taiwan IBV strains failed to show any HA activity after the neuraminidase treatment under standard or optimized protocols. This difference is not genotype-dependent. The amount of S1 gene did not equivalently correlate to the obtained HA titers. The presence of S1 protein in the prepared HA antigens was verified by western blot. The present study provides the information on the diversity of HA activity that Taiwan IBVs display and primary investigation on the factors that may influence the HA activity. Furthermore, to investigate the hemagglutination activity mediated by the S1 protein, the full S1 gene of the Taiwan IBV 2575/98 was cloned and expressed in Sf9 cells with the baculovirus expression vector system. Both of the recombinant S1 protein and the recombinant baculovirus possessed good reactivity with the chicken hyperimmune sera against several IBVs. Most notably, the baculovirus infected Sf9 cells acquired the ability to directly hemadsorb the chicken erythrocytes without neuraminidase treatment. The phenomenon of hemadsorption was inhibited by the IBV chicken antiserum, indicating the hemadsorption activity was specific induced by IBV-related proteins. However, no HA titer was obtained from any materials, even after additional neuraminidase treatment. This is the first observation of cell-associated hemadsorption activity in IBV research. This finding may provide a simple model for investigating the mechanism of virus-mediated hemagglutination activity, cell attachment and fusion.
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Chapitres de livres sur le sujet "Hemagglutination Assay"

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Sano, Kotone, et Haruko Ogawa. « Hemagglutination (Inhibition) Assay ». Dans Methods in Molecular Biology, 47–52. New York, NY : Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1292-6_4.

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Spackman, Erica, et Ioannis Sitaras. « Hemagglutination Inhibition Assay ». Dans Methods in Molecular Biology, 11–28. New York, NY : Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0346-8_2.

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Killian, Mary Lea. « Hemagglutination Assay for Influenza Virus ». Dans Methods in Molecular Biology, 3–9. New York, NY : Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0758-8_1.

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Killian, Mary Lea. « Hemagglutination Assay for Influenza Virus ». Dans Methods in Molecular Biology, 3–10. New York, NY : Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0346-8_1.

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Killian, Mary Lea. « Hemagglutination Assay for the Avian Influenza Virus ». Dans Avian Influenza Virus, 47–52. Totowa, NJ : Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-279-3_7.

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Zhang, Hong, et Ce Huang. « Application of Dot Immunobinding Assay (DIBA) and Reversed Passive Hemagglutination Assay (RPHA) in Detection of Shigella flexneri from Fecal Samples ». Dans Rapid Methods and Automation in Microbiology and Immunology, 104–10. Berlin, Heidelberg : Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-76603-9_12.

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Pedersen, Janice C. « Hemagglutination-Inhibition Assay for Influenza Virus Subtype Identification and the Detection and Quantitation of Serum Antibodies to Influenza Virus ». Dans Methods in Molecular Biology, 11–25. New York, NY : Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0758-8_2.

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BURLESON, FLORENCE G., THOMAS M. CHAMBERS et DANNY L. WIEDBRAUK. « HEMAGGLUTINATION ASSAY ». Dans Virology, 86–92. Elsevier, 1992. http://dx.doi.org/10.1016/b978-0-12-144730-4.50021-7.

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BURLESON, FLORENCE G., THOMAS M. CHAMBERS et DANNY L. WIEDBRAUK. « HEMAGGLUTINATION-INHIBITION ASSAY ». Dans Virology, 130–34. Elsevier, 1992. http://dx.doi.org/10.1016/b978-0-12-144730-4.50031-x.

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Potekaev, Nikolay, Olga Zhukova et Irina Khamaganova. « False-Positive Serologic Reactions for Syphilis ». Dans Bacterial Sexually Transmitted Infections - New Findings, Diagnosis, Treatment, and Prevention [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.106370.

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The epidemiologic situation of syphilitic infection warrants attention to diagnostic methods. Nontreponemal tests (rapid plasma regain, Venereal Disease Research Laboratory) are less reliable, as there are certain situations when false-positive reactions for syphilis antibodies may appear. Variable examinations were performed and proved that it was necessary to assess the titer of antibodies, as well as confirmation of the diagnosis by treponemal tests (fluorescent treponemal antibody, treponema pallidum hemagglutination assay, enzyme immunoassay, Western blot), were obligatory. In recent decades, new methods were elaborated (e.g., BioPlex total screen, tests with β2-GPI-dependent anticardiolipin antibody, the ARCHITECT syphilis treponema pallidum chemiluminescent immunoassay, the Elecsys immunoassay (Roche Diagnostics)). We present the review of publications on syphilis serologic diagnostics and present our own research. We did not find any mention of a false-positive test in atopic dermatitis and present a case of false-positive reactions for syphilis in such patients.
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Actes de conférences sur le sujet "Hemagglutination Assay"

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Luwito, Bagus Nanang, Ahmad Nadif, Retno D. Soejoedono et I. Wayan T. Wibawan. « Subtype Identification Of Avian Influenza Virus Isolated from Laying Duck In Sidenreng Rappang, South Sulawesi With Hemagglutination Inhibition Assay ». Dans 1st International Conference in One Health (ICOH 2017). Paris, France : Atlantis Press, 2018. http://dx.doi.org/10.2991/icoh-17.2018.28.

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Carr, JM, ML McKinney, I. Neuringer et J. McDonagh. « MONOCLONAL ANTIBODIES To D-DIMER : DISCREPANT LATEX AGGLUTINATION AND EIA RESULTS IN LIVER DISEASE PATIENTS ». Dans XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644836.

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Measurement of fibrin (ogen) degradation products (FDP) by latex agglutination with polyclonal antifibrinogen may be falsely elevated in liver disease due to the presence of “poorly clottable” fibrinogen in serum (Blood 67:1468, 1986). TSro monoclonal antibodies recognizing different epitopes of fibrin degradation fragment D-dimer (DD) are now commercially available. Because of the reported lack of crossreactivity of the DD monoclonals with fibrinogen, we employed them as tools to distinguish true fibrinolysis from false positive results due to liver disease. 17 citrated plasmas were evaluated by 5%SDS PAGE, nitrocellulose transfer and polyclonal antifibrinogen blotting for detection of FDP (X,ED,Y,D). Samples were then evaluated for ED by latex agglutination and by EIA (enzyme immunoassay) with the following results:Patients in Groups 3a and 3b were jaundiced (bilirubins 3.2-27) and had either hepatitis or cirrhosis except for one patient in Group 3a who had metastatic cancer. Assay for soluble fibrin monomer (SFM) by hemagglutination was negative in group 3b but positive in 5 of 7 patients in group 3a. Neither eta recognized fibrinogen. Purified fibrin monomer gave positive results with both EIAs. Detection of fibrinolysis by latex agglutination using monoclonal anti-DD resolves the problem of false positive results seen with polyclonal antifibrinogen in patients with liver disease. Elevated SFM in the absence of disseminated intravascular coagulation (DIC) in patients with liver disease may induce false positive ED results when measured by EIA.
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