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Auteur : Grafiati
Publié le 14 mars 2023

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1

Thomidis, T., et E. Exadaktylou. « First Report of Pilidiella granati on Pomegranate with Symptoms of Crown Rot in the Prefecture of Xanthi, Greece ». Plant Disease 95, no 1 (janvier 2011) : 79. http://dx.doi.org/10.1094/pdis-07-10-0514.

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In spring 2010, plants of pomegranate (Punica granatum L.) cv. Wonderful with symptoms of crown rot were observed in the Prefecture of Xanthi, Thrace, Greece. Close examination of these plants revealed distinct symptoms of crown rots. Isolations from the lower margins of the necrotic area were made by plating tissues of approximately 3 mm on acidified (2.5 ml of 85% lactic acid per liter of nutrient medium to create a pH = 3.5 after autoclaving) potato dextrose agar. The plates were incubated at 23°C for 5 to 7 days, and consistent colonies with light yellow, leathery mycelia and abundant, black, solitary pycnidia of various sizes were observed. Hyphae were septate and conidia were hyaline, one-celled, and ellipsoid to fusiform (average 10.1 to 20.2 × 3.2 to 4.3 μm). The pathogen was identified as Pilidiella granati Saccardo (synonym Coniella granati (Saccardo) Petrak & Sydow (3)) based on mycelium and spore morphology and ribosomal ITS1-5.8S-ITS2 sequences, which were identical to GenBank No. FN908875. Koch's postulates were completed in the laboratory by inoculating 20 1-year-old plants of pomegranate cv. Wonderful. With a 7-mm-diameter cork borer, a wound was created in the middle of each collar by removing the bark. A 6-mm-diameter agar plug bearing mycelia and spores from a 15-day-old culture of P. granati was inserted into each wound. The wound was covered with petroleum jelly and wrapped with adhesive tape to prevent desiccation. Ten trees were inoculated with sterile potato dextrose agar plugs to serve as controls. All plants were incubated at 25°C for 10 days, at which time necrosis was observed. Koch's postulates were satisfied after reisolating the fungus from inoculated plants that developed symptoms similar to those observed in the field. Control plants produced no symptoms of disease. To our knowledge, this is the first report of P. granati from pomegranate plants with symptoms of crown rots in Greece. The role of predisposing factors such as herbicides and frost damage to infection by P. granati is unknown. This pathogen has been reported to cause fruit rot of pomegranate in Spain (2) and California (1). References: (1) T. J. Michailides et al. (Abstr.) Phytopathology 100(suppl.):S83, 2010. (2) L. Palou et al. New Dis. Rep. Online publication. doi:10.5197/j.2044-0588.2010.022.021, 2010. (3) G T. Tziros and K. Tzavella-Klonari. Plant Pathol. 57:783, 2007.
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Mincuzzi, Annamaria, Antonio Ippolito, Virginia Brighenti, Lucia Marchetti, Stefania Benvenuti, Angela Ligorio, Federica Pellati et Simona Marianna Sanzani. « The Effect of Polyphenols on Pomegranate Fruit Susceptibility to Pilidiella granati Provides Insights into Disease Tolerance Mechanisms ». Molecules 25, no 3 (24 janvier 2020) : 515. http://dx.doi.org/10.3390/molecules25030515.

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Pilidiella granati, also known as Coniella granati, is the etiological agent of pomegranate fruit dry rot. This fungal pathogen is also well-known as responsible for both plant collar rot and leaf spot. Because of its aggressiveness and the worldwide diffusion of pomegranate crops, the selection of cultivars less susceptible to this pathogen might represent an interesting preventive control measure. In the present investigation, the role of polyphenols in the susceptibility to P. granati of the two royalties-free pomegranate cultivars Wonderful and Mollar de Elche was compared. Pomegranate fruit were artificially inoculated and lesion diameters were monitored. Furthermore, pathogen DNA was quantified at 12–72 h post-inoculation within fruit rind by a real time PCR assay setup herein, and host total RNA was used in expression assays of genes involved in host-pathogen interaction. Similarly, protein extracts were employed to assess the specific activity of enzymes implicated in defense mechanisms. Pomegranate phenolic compounds were evaluated by HPLC-ESI-MS and MS2. All these data highlighted ‘Wonderful’ as less susceptible to P. granati than ‘Mollar de Elche’. In the first cultivar, the fungal growth seemed controlled by the activation of the phenylpropanoid pathway, the production of ROS, and the alteration of fungal cell wall. Furthermore, antifungal compounds seemed to accumulate in ‘Wonderful’ fruit following inoculation. These data suggest that pomegranate polyphenols have a protective effect against P. granati infection and their content might represent a relevant parameter in the selection of the most suitable cultivars to reduce the economic losses caused by this pathogen.
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Kwon, Jin-Hyeuk, et Chang-Seuk Park. « Fruit Rot of Pomegranate (Punica granatum) Caused by Coniella granati in Korea ». Research in Plant Disease 8, no 4 (1 décembre 2002) : 215–19. http://dx.doi.org/10.5423/rpd.2002.8.4.215.

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4

Uysal, Aysun, Şener Kurt, Emine Mine Soylu, Merve Kara et Soner Soylu. « Morphology, Pathogenicity and Management of Coniella Fruit Rot (Coniella granati) on Pomegranate ». Turkish Journal of Agriculture - Food Science and Technology 6, no 4 (10 avril 2018) : 471. http://dx.doi.org/10.24925/turjaf.v6i4.471-478.1787.

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One of the objectives of the study was to identify the fungus involved in fruit rot on pomegranates in the Mediterranean Region of Turkey. The fungus designated as Coniella granati (Saccardo) Petrak and Sydow based on morphological characteristics. The fungus colonized the fruit after 5 to 8 days, followed by the appearance of fruit rot symptoms leading to the formation of abundant pycnidia covering the peel. Secondly, the efficacy of fungicides against C. granati was evaluated by mycelial growth and conidial germination assays. Tebuconazole, boscalid+pyraclostrobin and iprodione at 1.0, 25, and 50 μgml-1 concentrations, respectively, completely inhibited mycelial growth. In the azoxystrobin and dodine, relatively higher concentrations required to inhibit mycelial growth. Tebuconazole exhibited the greatest inhibition (82.2%) of mycelium growth. The EC50 values in mycelial growth of C. granati ranged from 0.13 to 151.9. The highest EC50 values occurred for tebuconazole (0.13μgml-1). Tebuconazole, boscalid+pyraclostrobin and iprodione at 200, 10 and 5 μgml-1 concentrations, respectively, were the highly effective in inhibiting conidial germination. Azoxystrobin exhibited a low effect (61%) on conidial germination. The EC50 values on conidial germination of C. granati ranged from 0.2 to 28.7. Tebuconazole had the lowest EC50 value, while boscalid+pyraclostrobin exhibited the highest EC50 value.
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5

Yuan, Hongbo, Hui Hou, Tianxiang Huang, Zengqiang Zhou, Hongtao Tu et Li Wang. « Agrobacterium tumefaciens-mediated transformation of Coniella granati ». Journal of Microbiological Methods 182 (mars 2021) : 106149. http://dx.doi.org/10.1016/j.mimet.2021.106149.

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Tekiner, Nasibe, Recep Kotan, Elif Tozlu et Fatih Dadaşoğlu. « Biological Control of Coniella granati Saccardo in Pomegranate ». Universal Journal of Agricultural Research 8, no 1 (janvier 2020) : 18–24. http://dx.doi.org/10.13189/ujar.2020.080103.

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7

Al-Sweedi, Taha M. M. « The Accumulated Heat Units Required for the Development of the Different Stages of the Grape False Spider Mite, Tenuipalpus granati Sayed Under Laboratory Conditions ». Arab Journal for Plant Protection 40, no 4 (2022) : 351–55. http://dx.doi.org/10.22268/ajpp-40.4.351355.

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Al-Sweedi, T.M.M. 2022. The Accumulated Heat Units Required for the Development of the Different Stages of the Grape False Spider Mite, Tenuipalpus granati Sayed Under Laboratory Conditions. Arab Journal of Plant Protection, 40(4): 351-355. https://doi.org/10.22268/AJPP-40.4.351355 The grape false spider mite Tenuipalpus granati Sayed (Acariformes: Tenuipalpidae) is one of the significant pests on the grape trees in Iraq. The aim of this study was to determine the threshold temperature and calculate the heat units needed for the development time of the duration of this mite stage. Different incubation temperatures (10 15, 20, 25, 30, 35, and 40±2°C), relative humidity (50-60±5%), and light:darkness period of 16: 8 hours were applied to rear this mite on the lower surface of the newly grown grape leaves. The results showed that the development time of the egg, larva, protonymph, and deutonymph (active, quiescent, active + quiescent) at temperatures 15, 20, 25, 30, and 35°C were decreased gradually with temperature increase. Furthermore, the development time of the eggs incubation period was 15.3, 4.98 days and the larva (active + quiescent) were 15.1, 4.43 days, and the protonymph (active + quiescent) was 14.4, 3.48 days and of the deutonymph (active + quiescent) was 15.6, 5.58 days for the effect of 15 and 35°C. Additionally, the development time from egg to adult was 60.00 and 18.26 days at 15 and 35°C, respectively. The threshold temperature was 6.73, 7.89, 9.38, 6.47 and 7.3 °C, respectively. Finally, the accumulated heat units required for the development time of each mite instar varied according to the threshold temperature difference and development time. Keywords: False red mite, heat units, Tenuipalpus granai, developmental rate.
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8

Mirabolfathy, M., J. Z. Groenewald et P. W. Crous. « First Report of Pilidiella granati Causing Dieback and Fruit Rot of Pomegranate (Punica granatum) in Iran ». Plant Disease 96, no 3 (mars 2012) : 461. http://dx.doi.org/10.1094/pdis-10-11-0887.

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Iran is the largest producer of pomegranate (Punica granatum) in the world, with more than 60,000 ha currently in production. In the spring of 2011, a decline and dieback of young pomegranate trees (7 to 10 years old) were observed in the Kheir area of Fars Province. Dieback and twig blight developed toward the lower part of the stem, resulting in death of aerial tree parts and growing suckers from roots. Surface-disinfected tissues of diseased plants were plated on potato dextrose agar (PDA) and malt extract agar media. Isolates were separated into two groups that had either pale green or white aerial mycelia and sporulated after 5 to 7 days at 25°C. Pycnidia were globose and black with thin, membranous, pseudoparenchymatic walls, 80 to 140 μm in diameter. Conidia were hyaline, one-celled, elongate to fusiform, straight, and 11 to 17 × 4 to 6 μm (average 14 × 4.7 μm). Cardinal minimum growth temperatures were 8 to 10°C, optimum at 27 to 30°C, and maximum at 35°C. Radial growth rate at 30°C was 8 to 9 mm per day. Representative isolates were deposited in the CBS-KNAW Fungal Biodiversity Centre, the Netherlands (CPC 19625 = CBS 130974 and CPC 19626 = CBS 130975; GenBank JN815312 and JN815313, respectively). Genomic DNA was extracted with the UltraClean Microbial DNA Isolation Kit (MoBio Laboratories, Inc., Solana Beach, CA) and the internal transcribed spacer (ITS) region of the nrDNA operon of two isolates were sequenced as described previously (1). On the basis of morphology (3), the causal organism was identified as Pilidiella granati Sacc. This identification was corroborated by the ITS sequence data, which was identical for both colony types to GenBank HQ166057 (identities = 614 of 614 [100%]). Pathogenicity tests were conducted using two representative isolates from each group on 5-month-old P. granatum trees with 10 replicates under greenhouse conditions; 5-mm mycelial plugs from the edge of 7-day-old colonies on PDA were placed under the bark of twig wounds. Uncolonized PDA plugs were used as noninoculated controls. Pathogenicity was also tested on nonwounded fruit by placing colonized 5-mm-diameter mycelial plugs on surface-disinfected pomegranate fruits; noncolonized PDA plugs were used as controls. All treated fruit were placed in plastic bags and maintained at 25°C for 10 days. Isolates were found to be pathogenic on twigs after 2 months, giving rise to brown lesions that were 2 to 5 cm long. No lesions were observed on the controls. Furthermore, the fungus was reisolated from all infected tissues, satisfying Koch's postulates. On pomegranate fruit, the fungus colonized the fruit after 5 to 8 days, followed by the appearance of fruit rot symptoms leading to the formation of abundant pycnidia covering the skin after 10 days. No decay was observed in control inoculations. Pilidiella granati has previously been reported as a pathogen of P. granatum fruit from Europe, Asia, and the United States (2). To our knowledge, this is the first report of this pathogen causing dieback and fruit rot of pomegranate in Iran. References: (1) J. Frank et al. Persoonia 24:93, 2010. (2) L. Palou et al. Online publication. doi:10.5197/j.2044.0588.2010.022.021. New Dis. Rep. 22:21, 2010. (3) J. M. Van Niekerk et al. Mycol. Res. 108:283, 2004.
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Park, Soo-Yeon, Chang-Su Na, Won-Cheol Jeong et Jong-Cheol Lee. « A Literature Study of Pericarpium Granati and Cortex Betulae Platyphyllae ». Journal of Korean Oriental Medical Ophthalmology and Otolaryngology and Dermatology 25, no 3 (25 août 2012) : 13–33. http://dx.doi.org/10.6114/jkood.2012.25.3.013.

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Çeliker, Naciye Mükerrem, Ayşe Uysal, Barbaros Çetinel et Dilek Poyraz. « Crown rot on pomegranate caused by Coniella granati in Turkey ». Australasian Plant Disease Notes 7, no 1 (16 octobre 2012) : 161–62. http://dx.doi.org/10.1007/s13314-012-0074-6.

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Chen, Y., D. D. Shao, A. F. Zhang, X. Yang, M. G. Zhou et Y. L. Xu. « First Report of a Fruit Rot and Twig Blight on Pomegranate (Punica granatum) Caused by Pilidiella granati in Anhui Province of China ». Plant Disease 98, no 5 (mai 2014) : 695. http://dx.doi.org/10.1094/pdis-09-13-1012-pdn.

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Pomegranate, Punica granatum Linn., is widely planted as an ornamental and a fruit crop in Huaiyuan County, Anhui Province, which is the primary pomegranate production area in China. In the early summer of 2012, twig dieback and fruit rot were observed on about 10% and 30% of the pomegranate trees, respectively, in several villages of Huaiyuan County. Necrosis was observed in the twigs, resulting in death of the branches. On fruit, dry rot started at the sepals, covered the entire surface in severely infected fruit, and eventually resulted in shriveling of the fruit. Abundant, black, and solitary pycnidia were observed on diseased twigs and fruit. Pieces of tissue (3 mm in size) from diseased twigs and sepals were surface-disinfected in 75% ethanol for 1 min, washed in sterile water three times, plated on potato dextrose agar (PDA) acidified with 2.5 ml 85% lactic acid per liter, and incubated at 25°C. Resulting fungal cultures produced pale green or white aerial mycelia and sporulated after 5 to 7 days. Pycnidia, ~80 to 130 μm in diameter, were globose and black with thin and membranous walls and contained hyaline, one-celled, and ellipsoid to fusiform conidia, averaging 10.8 to 17.2 × 2.9 to 4.7 μm in size. These morphological features were consistent with Pilidiella granati Sacc. (= Coniella granati Sacc.) (2). Genomic DNA from each of the 10 isolates was extracted and purified using a DNA Gel Extraction Kit (AxyPrep, Hangzhou, China), and PCR was conducted using a DNA Engine System PTC-200 (BIO-RAD, Watertown, MA) with ITS1 and ITS4 internal transcribed spacer universal primers. A single 616-bp fragment was amplified from all 10 isolates and sequenced. Sequence analysis revealed that the ITS from these isolates were identical (100% similarity, GeneBank Accession No. KF560320) to each other and showed >99.5% similarity with those of the P. granti isolates deposited in GenBank (AY339342.1). To evaluate pathogenicity, mycelial plugs (5 mm diameter) from 7-day-old PDA cultures were transferred onto the non-wounded sepals of pomegranate fruit (one plug per fruit, six fruits per isolate), and then all inoculated fruit were placed in plastic bags and maintained at 25°C for 14 days. In addition, twigs on pomegranate plant growing in the field were inoculated by placing mycelial plugs of the fungus on young bark and covered with cotton saturated with sterile water (one plug per twig, six twigs per isolate). Sterile PDA plugs were used as controls in both tests. All 10 isolates colonized the fruit after 5 to 8 days; this was followed by the appearance of dry rot and formation of abundant pycnidia after 10 to 12 days. No decay was observed on the control fruit. Isolates were also pathogenic on twigs, resulting in brown lesions after 2 months that were 2 to 5 cm long. No lesions were observed on the control twigs. Furthermore, the pathogen was isolated from all inoculated fruit and twig tissues and identified to be P. granati as described above, fulfilling Koch's postulates. This pathogen has been reported in Spain (3), Greece (4), and Iran (1), causing crown rot on pomegranate in addition to infecting fruit, but has not been reported previously in Anhui Province of China. This disease is an emerging problem in Anhui Province and will necessitate the development of new disease management practices to sustain commercial production in this region. References: (1) M. Mirabolfathy et al. Plant Dis. 96:461, 2012. (2) Niekerk et al. Mycol. Res. 108:283, 2004. (3) L. Palou et al. New Dis. Rep. 22:21, 2010. (4) T. Thomidis et al. Plant Dis. 95:79, 2011.
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Mahadevakumar, Shivannegowda, Mahadevappa Shreenidhi et Gottravalli Ramanayaka Janardhana. « First report of Coniella granati associated with dieback and fruit rot of pomegranate (Punica granatum L.) in India ». Journal of Plant Pathology 101, no 3 (12 février 2019) : 787. http://dx.doi.org/10.1007/s42161-019-00256-z.

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Thomidis, Thomas. « Pathogenicity and characterization of Pilidiella granati causing pomegranate diseases in Greece ». European Journal of Plant Pathology 141, no 1 (28 août 2014) : 45–50. http://dx.doi.org/10.1007/s10658-014-0520-8.

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Levy, Edna, Genya Elkind, Ruth Ben-Arie et I. S. Ben-Ze’ev. « First report of Coniella granati causing pomegranate fruit rot in Israel ». Phytoparasitica 39, no 4 (1 juin 2011) : 403–5. http://dx.doi.org/10.1007/s12600-011-0171-7.

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Ueckermann, Edward A., Eric Palevsky, Uri Gerson, Eitan Recht et Pieter D. Theron. « The Tenuipalpidae (Acari : Trombidiformes) of Israel ». Acarologia 58, no 2 (12 avril 2018) : 483–525. http://dx.doi.org/10.24349/acarologia/20184255.

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An annotated list and key to the 26 species of phytophagous false spider mites (Trombidiformes: Tenuipalpidae) known from Israel is provided. About two thirds are exotics, having invaded Israel within the last 35 years. Eight species, namely Brevipalpus californicus, B. lewisi, B. obovatus, B. phoenicis, B. yothersi, Raoiella indica, Tenuipalpus granati and T. punicae are agricultural pests. The others have little economic impact, or are possibly controlled by natural enemies. A key to all the species is given.
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Hatzinikolis, Ε. Ν. « The genus Tenuipalpus (Acari : Tenuipalpidae) in Greece ». ENTOMOLOGIA HELLENICA 4 (29 mai 2017) : 19. http://dx.doi.org/10.12681/eh.13928.

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Knowledge of the Greek mites of the genus Tenuipaipus was limited to the following two species only: T. caudatus (Doges) and T. granati Sayed. Recent studies revealed the presence of another five species namely: T. crassus Andre, T. pacificus Baker, T. punicae Pritchard and Baker, T. rosae Kadzhava and T. zhizhilashviliae Reek. A key to the seven species now recorded from Greece is presented. Some information concerning the distribution, economic importance and host plants of the above mentioned Tenuipalpus species is also given.
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Mincuzzi, Annamaria, Simona Marianna Sanzani, Lluís Palou, Marco Ragni et Antonio Ippolito. « Postharvest Rot of Pomegranate Fruit in Southern Italy : Characterization of the Main Pathogens ». Journal of Fungi 8, no 5 (30 avril 2022) : 475. http://dx.doi.org/10.3390/jof8050475.

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Pomegranate (Punica granatum L.) is an emerging crop in Italy and particularly in southern regions, such as Apulia, Basilicata, and Sicily, due to favorable climatic conditions. The crop is affected by several pathogenic fungi, primarily in the field, but also during postharvest phases. The most important postharvest fungal diseases in pomegranate are gray and blue molds, black heart and black spot, anthracnose, dry rot, and various soft rots. The limited number of fungicides allowed for treatment in the field and the lack of postharvest fungicides make it difficult to control latent, quiescent, and incipient fungal infections. Symptomatic pomegranates from southern Italy were sampled and isolated fungi were morphologically and molecularly characterized. The data obtained revealed that various species of Penicillium sensu lato (including Talaromyces genus), Alternaria spp., Coniella granati, and Botrytis cinerea were the principal etiological agents of postharvest pomegranate fruit diseases; other relevant pathogens, although less represented, were ascribable to Aspergillus sect. nigri, Colletotrichum acutatum sensu stricto, and Cytospora punicae. About two thirds of the isolated pathogens were responsible for latent infections. The results obtained may be useful in planning phytosanitary control strategies from the field to storage, so as to reduce yield losses.
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Fanelli, Giuliano, Michele De Sanctis, Ermelinda Gjeta, Alfred Mullaj et Fabio Attorre. « The Vegetation of the Buna River Protected Landscape (Albania) ». Hacquetia 14, no 2 (1 décembre 2015) : 129–74. http://dx.doi.org/10.1515/hacq-2015-0008.

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AbstractThe vegetation of Buna River Protected Landscape is described. The area comprises both the alluvial plain of the lower course of the Buna river that marks the boundary between Montenegro and Albania, and a carbonatic range. The vegetation is characterized by a high β-diversity (27 alliances and 46 associations), especially in wetlands and dry grasslands. In the area it is possible to distinguish 1 dunal and 4 hygro-sequences in the alluvial plain and 3 xeroseries in the carbonatic range. Associations Clematido viticellae-Punicetum granati and Periploco graecae-Alnetum glutinosae are described as new.
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Wang, Bor-Sen, Kuen-Lin Leu, Guan-Jhong Huang, Ching-Fen Yeh, Huo-Mu Tai, Wen-Yueh Ho et Ming-Hsing Huang. « Protective effects of an aqueous Pericarpium Granati extract against inflammatory damage in mice ». Journal of Functional Foods 9 (juillet 2014) : 183–91. http://dx.doi.org/10.1016/j.jff.2014.04.022.

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Zhou, Xin-Yu, Rui-Lin Liu, Xue Ma et Zhi-Qi Zhang. « Polyethylene glycol as a novel solvent for extraction of crude polysaccharides from pericarpium granati ». Carbohydrate Polymers 101 (janvier 2014) : 886–89. http://dx.doi.org/10.1016/j.carbpol.2013.10.017.

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Madufor, N. J. K., L. Page, J. Burger, W. J. Perold et U. L. Opara. « Design and fabrication of a portable potentiostat for DNA-based electrochemical detection of Coniella granati in pomegranate (Punica granatum) fruit ». Acta Horticulturae, no 1349 (octobre 2022) : 357–64. http://dx.doi.org/10.17660/actahortic.2022.1349.49.

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KC, A. N., et G. E. Vallad. « First Report of Pilidiella granati Causing Fruit Rot and Leaf Spots on Pomegranate in Florida ». Plant Disease 100, no 6 (juin 2016) : 1238. http://dx.doi.org/10.1094/pdis-09-15-1054-pdn.

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Mohanan, C., K. Rajesh Kumar et K. Yesodharan. « Coniella causing foliage diseases in forest species in Kerala, India ». Indian Journal of Forestry 33, no 3 (1 septembre 2010) : 355–60. http://dx.doi.org/10.54207/bsmps1000-2010-rfcqv0.

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Coniella australiensis, C. fragariae, C. granati, C. minima and C. petrakii causing foliage infection in 18 forest species in natural forests, plantations and forest nurseries in Kerala State, India are reported. Six species of Eucalypts, E. camaldulensis, E. citriodora, E. grandis, E. pellita, E. urophylla, E. tereticornis and many clones and provenances of E. tereticornis were found affected with various species of Coniella. Careya arborea, Cleistanthus collinus, Garcinia gummi-gutta, Gmelina arborea, Macaranga peltata, Mikania micrantha, Myristica sp., Persea macrantha, Tabernaemontana heyniana, Terminalia chebula, Terminalia paniculata, Syzygium caryophyllatum were the other host plants affected with Coniella species. Among the five species of Coniella affecting the forest tree species, C. fragariae was the most widespread and predominant pathogen and caused foliage disease in 12 forest tree species.
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Ruhimat, Udang. « UJI IN-VITRO AKTIVITAS ANTIBAKTERI INFUSUM KULIT BUAH DELIMA (Granati Pericarpium cortex) TERHADAP BAKTERI Escherichia coli ». Jurnal Kesehatan Bakti Tunas Husada : Jurnal Ilmu-ilmu Keperawatan, Analis Kesehatan dan Farmasi 9, no 1 (10 juin 2015) : 75. http://dx.doi.org/10.36465/jkbth.v9i1.97.

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Pollastro, S., C. Dongiovanni, D. Gerin, P. Pollastro, G. Fumarola, R. M. De Miccolis Angelini et F. Faretra. « First Report of Coniella granati as a Causal Agent of Pomegranate Crown Rot in Southern Italy ». Plant Disease 100, no 7 (juillet 2016) : 1498. http://dx.doi.org/10.1094/pdis-11-15-1320-pdn.

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Facchinelli, Valerio, et Mario Gaeta. « Indicazioni petrogenetiche dai granati birifrangenti dei proietti sialici nelle vulcaniti alcalino potassiche dei Monti Sabatini (Lazio) ». Rendiconti Lincei 3, no 4 (décembre 1992) : 295–310. http://dx.doi.org/10.1007/bf03002937.

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Nerya, O., A. Tzviling, H. Hibrahim et R. Ben-Arie. « Coniella granati – a new pathogen of pomegranates in Israel – postharvest fungicide screening for control of storage decay ». Acta Horticulturae, no 1144 (novembre 2016) : 465–68. http://dx.doi.org/10.17660/actahortic.2016.1144.69.

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Thomidis, T., et A. Filotheou. « Evaluation of five essential oils as bio-fungicides on the control of Pilidiella granati rot in pomegranate ». Crop Protection 89 (novembre 2016) : 66–71. http://dx.doi.org/10.1016/j.cropro.2016.07.002.

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Munhuweyi, K., C. L. Lennox, J. C. Meitz-Hopkins, O. J. Caleb, G. O. Sigge et U. L. Opara. « In vitro effects of crab shell chitosan against mycelial growth of Botrytis sp., Penicillium sp. and Pilidiella granati ». Acta Horticulturae, no 1144 (novembre 2016) : 403–8. http://dx.doi.org/10.17660/actahortic.2016.1144.60.

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Lennox, C. L., L. Mostert, E. Venter, W. Laubscher et J. C. Meitz-Hopkins. « First Report of Coniella granati Fruit Rot and Dieback on Pomegranate in the Western Cape of South Africa ». Plant Disease 102, no 4 (avril 2018) : 821. http://dx.doi.org/10.1094/pdis-09-17-1387-pdn.

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Brighenti, Virginia, Ramona Iseppi, Luca Pinzi, Annamaria Mincuzzi, Antonio Ippolito, Patrizia Messi, Simona Marianna Sanzani, Giulio Rastelli et Federica Pellati. « Antifungal Activity and DNA Topoisomerase Inhibition of Hydrolysable Tannins from Punica granatum L. » International Journal of Molecular Sciences 22, no 8 (17 avril 2021) : 4175. http://dx.doi.org/10.3390/ijms22084175.

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Punica granatum L. (pomegranate) fruit is known to be an important source of bioactive phenolic compounds belonging to hydrolysable tannins. Pomegranate extracts have shown antifungal activity, but the compounds responsible for this activity and their mechanism/s of action have not been completely elucidated up to now. The aim of the present study was the investigation of the inhibition ability of a selection of pomegranate phenolic compounds (i.e., punicalagin, punicalin, ellagic acid, gallic acid) on both plant and human fungal pathogens. In addition, the biological target of punicalagin was identified here for the first time. The antifungal activity of pomegranate phenolics was evaluated by means of Agar Disk Diffusion Assay and minimum inhibitory concentration (MIC) evaluation. A chemoinformatic analysis predicted for the first time topoisomerases I and II as potential biological targets of punicalagin, and this prediction was confirmed by in vitro inhibition assays. Concerning phytopathogens, all the tested compounds were effective, often similarly to the fungicide imazalil at the label dose. Particularly, punicalagin showed the lowest MIC for Alternaria alternata and Botrytis cinerea, whereas punicalin was the most active compound in terms of growth control extent. As for human pathogens, punicalagin was the most active compound among the tested ones against Candida albicans reference strains, as well as against the clinically isolates. UHPLC coupled with HRMS indicated that C. albicans, similarly to the phytopathogen Coniella granati, is able to hydrolyze both punicalagin and punicalin as a response to the fungal attack. Punicalagin showed a strong inhibitory activity, with IC50 values of 9.0 and 4.6 µM against C. albicans topoisomerases I and II, respectively. Altogether, the results provide evidence that punicalagin is a valuable candidate to be further exploited as an antifungal agent in particular against human fungal infections.
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NEELAM KUMARI et VED RAM. « Influence of epidemiological parameters on the development and spread of leaf spot and dry fruit rot (Coniella granati) of pomegranate ». Journal of Agrometeorology 17, no 2 (1 décembre 2015) : 259–60. http://dx.doi.org/10.54386/jam.v17i2.1021.

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Xu, Xinya, Ruilin Liu, Pengqi Guo, Zhimin Luo, Xiang Cai, Hua Shu, Yanhui Ge, Chun Chang et Qiang Fu. « Fabrication of a novel magnetic mesoporous molecularly imprinted polymer based on pericarpium granati-derived carrier for selective absorption of bromelain ». Food Chemistry 256 (août 2018) : 91–97. http://dx.doi.org/10.1016/j.foodchem.2018.02.118.

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Baral, KP, et P. Basnet. « Antioxidant Activity of Selected Natural Medicines used in Nepal ». Journal of Chitwan Medical College 3, no 2 (13 août 2013) : 27–31. http://dx.doi.org/10.3126/jcmc.v3i2.8439.

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Diminished antioxidant defence or increased production of reactive oxygen and nitrogen species in the biological system can result into oxidative stress which can cause damage to deoxyribonucleic acid (DNA), proteins, lipids and as a result different disease states arise like cancer, neurodegenerative diseases, rheumatoid arthritis. Antioxidants from different plant resources can significantly delay or prevent oxidation of the substrate and hence prevents from various diseases. Therefore, present research was focused in search of potent natural antioxidants. For the study, methanolic extracts of twenty-five common natural medicines, mostly spices were screened using 1,1–diphenyl–2–picrylhydrazyl (DPPH) radical for their antioxidative activities. Among them, extracts of Chebulae Fructus, Terminalia Billericae Fructus, Phyllanthi Fructus, Cinnamomi Cortex, Arecae Semen, Pericarpium Punicae Granati, Syzygiae Fructus, Rhei Rhizoma, Pterocarpi Lignum and Santali Lignum Albi showed potent antioxidative activity with EC50 values being 1.5, 2.1, 1.4, 2, 1.5, 1.45, 2.7, 2.9, 3, 3.8 μg/mL, respectively. Ascorbic acid (EC50: 2.6 μg/mL) was used as positive control. Therefore, consumers can increase their intake of foods rich in antioxidant compounds that can lower the risk of chronic health problems DOI: http://dx.doi.org/10.3126/jcmc.v3i2.8439 Journal of Chitwan Medical College Vol.3(2) 2013 27-31
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Jiang, Xudong, Weihao Yang, Chuanjian Zhou, Kailing Lu et Cuiwu Lin. « Separation and Purification of Polyphenols from Pericarpium Granati Using Macroporous Resins and Evaluation of its Anti-Streptococcus mutans Activity in vitro ». Biotechnology(Faisalabad) 15, no 3-4 (15 avril 2016) : 86–95. http://dx.doi.org/10.3923/biotech.2016.86.95.

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Yang, Xue, Chun-Yan Gu, Muhammad Abid, Mohamed N. Al-Attala, Gai-Hua Qin, Yi-Liu Xu, Seinn Sandar May Phyo, Ai-Fang Zhang, Hao-Yu Zang et Yu Chen. « Development of loop-mediated isothermal amplification assay for rapid diagnosis of pomegranate twig blight and crown rot disease caused by Coniella granati ». Crop Protection 135 (septembre 2020) : 105190. http://dx.doi.org/10.1016/j.cropro.2020.105190.

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Liu, Rui-Lin, Zhi-Qi Zhang, Wang-Hui Jing, Lu Wang, Zhi-Min Luo, Rui-Miao Chang, Ai-Guo Zeng, Wei Du, Chun Chang et Qiang Fu. « β-Cyclodextrin anchoring onto pericarpium granati-derived magnetic mesoporous carbon for selective capture of lopid in human serum and pharmaceutical wastewater samples ». Materials Science and Engineering : C 62 (mai 2016) : 605–13. http://dx.doi.org/10.1016/j.msec.2016.02.004.

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Majchrzak, Jakub. « Myśl Wincentego Granata wobec problemów współczesności ». Filozofia Chrześcijańska 17 (30 juillet 2021) : 179–98. http://dx.doi.org/10.14746/fc.2020.17.9.

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The aim of the article is to present relevance of the personalistic Granat’s thought in the context of contemporary social challenges. In the article, I analyze the topic of Christian humanism in Granat’s thought. I point out that he saw the sources of this concept in man’s aspirations to learn the full truth about himself. Granat saw the final answer to these desires in the relation of man and the sense of his existence to the person of Christ. I also consider Granat’s opinion about the role of the Catholic church today. I would like to draw your attention to the fact that Granat clearly emphasized the deep spiritual and apostolic dimension of the Church, pointing out that the Church is above all the Mystical Body of Christ. Therefore, the fundamental task of the Church is to unite God’s children around Christ. The Church also has a duty to proclaim to man that he cannot understand himself without Christ. The Church accomplishes these goals by administering the sacraments and moderating interreligious dialogue. I consider the issue of peace in his philosophical refl ection as well. According to Granat the source of peace is God. Man drawing his strength from his closeness with God can contribute to peace through mutual respectful relationships. At the state level the key role is played by the concern of each country for the common good and international cooperation in this field. In conclusion, I formulate summarizing remarks.
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Zhou, Xin-Yu, Jing Zhang, Rui-Ping Xu, Xue Ma et Zhi-Qi Zhang. « Aqueous biphasic system based on low-molecular-weight polyethylene glycol for one-step separation of crude polysaccharides from Pericarpium granati using high-speed countercurrent chromatography ». Journal of Chromatography A 1362 (octobre 2014) : 129–34. http://dx.doi.org/10.1016/j.chroma.2014.08.034.

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Rojas-Rodríguez, Freddy, et Gustavo Torres-Córdoba. « Árboles del Valle Central de Costa Rica : reproducción Granada (Punica granatum L) ». Revista Forestal Mesoamericana Kurú 14, no 35 (26 juin 2017) : 84. http://dx.doi.org/10.18845/rfmk.v14i35.3158.

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Se presenta una breve descripción del árbol granada Punica granatum L, información sobre fenología como base para la recolección de frutos, el manejo de las semillas, la viverización y el proceso de germinación.
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Nizama-Chapoñan, Angie, Osni Rodriguez-Liñan et Luz María Paucar-Menacho. « Granada (Punica granatum) : una superfruta funcional con propiedades terapéuticas ». Agroindustrial Science 10, no 3 (30 décembre 2020) : 311–18. http://dx.doi.org/10.17268/agroind.sci.2020.03.14.

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García Aguilar, Mónica. « La construcción del espacio épico en Il Conquisto di Granata de Girolamo Graziani ». IRIS, no 35 (30 juin 2014) : 105–16. http://dx.doi.org/10.35562/iris.1777.

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A mediados del siglo xvii, Girolamo Graziani publica en Módena Il Conquisto di Granata (1650), un poema épico de 26 cantos en los que se narran los acontecimientos que rodearon el asedio del Reino de Granada en sus últimos diez años. Este poema, por tanto, describe el ambiente caballeresco y el tema de la lucha entre cristianos y musulmanes a través de múltiples episodios en los que no faltan los fieros y gigantescos moros, las doncellas guerreras y toda suerte de fábulas y encantamientos. En este estudio determinaremos la arquitectura espacial de este poema épico, definiendo la ciudad de Granada como centro de todo movimiento, en el que todas las acciones se valorarán y medirán a partir de la distancia y de la relación que tengan con ese lugar.
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Guerreo Solano, José Antonio, et Tania Flores Bazán. « Punica protopunica Balf., la hermana (¿abuela?) olvidada de la granada común : Generalidades y propiedades farmacológicas ». Quimiofilia 2, no 1 (30 novembre 2022) : 28–30. http://dx.doi.org/10.56604/qfla2022122830.

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El objetivo del presente es brindar información acerca de la granada de Socotra (Punica protopunica Balf.), una de las dos únicas especies del género Punica. Gran parte de la población humana la desconoce pues poco se ha publicado acerca de ella y solo su “hermana”, la granada común (Punica granatum L.), se ha extendido por el mundo. Es una especie endémica y aislada, que se encuentra en un archipiélago del océano Índico noroccidental. Esta obra nos lleva a un paseo a través de su taxonomía, morfología, cultivo, genética, vulnerabilidad, usos y sus propiedades farmacológicas, así como potencial farmacológico.
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Campos, Emannuel Ítalo Alves, Liliane de Sousa Silva, Sandra Alves de Sousa Garcia, Pollyana Guimarães de Oliveira, Milton Adriano Pelli de Oliveira, Carla Afonso da Silva et José Realino de Paula. « Atividade antimicrobiana do extrato, frações e punicalagina da casca do fruto de Punica granatum frente a isolados clínicos de vacas com mastite ». Research, Society and Development 10, no 16 (18 décembre 2021) : e531101623935. http://dx.doi.org/10.33448/rsd-v10i16.23935.

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O objetivo deste estudo foi avaliar a atividade antimicrobiana do extrato, frações e punicalagina purificada da casca do fruto da romã, das variedades granada e wonderful, frente a Staphylococcus Coagulase Positivo (SCP) e Staphylococcus Coagulase Negativa (SCN), isoladas de vacas com mastite. As cascas dos frutos de romã foram secas em estufa, moídas e o extrato etanólico foi preparado por maceração e percolação. As frações foram obtidas por fracionamento líquido-líquido, e a purificação da punicalagina realizada em coluna cromatográfica preenchida com Diaion® HP-20. CLAE e RMN (1H e 13C -Acetona-d6) foram utilizados para identificação, quantificação e elucidação estrutural da punicalagina e a CIM (CLSI-M7A10) foi determinada para 23 isolados clínicos. O teor de punicalagina foi maior no extrato e frações da variedade granada, chegando a 81,5% na amostra de punicalagina purificada, que apresentou boa atividade antimicrobiana frente aos isolados clínicos, com ênfase para S. aureus e S. schleiferi schleiferi, onde a CIM foi de 31,75 μg/mL. Desta forma. a punicalagina foi definida como importante metabólito para o potencial antimicrobiano dos frutos de P. granatum contudo, o sinergismo dos metabolitos das frações acetato de etila e fração aquosa da variedade granada, teve considerável importância para a boa atividade antimicrobiana dessas frações, frente a 100% dos SCP e SCN. Assim, os resultados obtidos confirmam a atividade antimicrobiana dos metabólitos presentes na casca da romã, podendo fundamentar novas pesquisas de formulações farmacêuticas baseadas em P. granatum, como alternativa para o tratamento, prevenção e controle da mastite.
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Bell Cortez, Carlos Alejandro. « Efecto del extracto hidroalcohólico del fruto de granada (Punica granatum L.) presentada en forma de gel farmacéutico en el tratamiento de las hemorroides ». Ciencia y Desarrollo 18, no 1 (5 août 2016) : 15. http://dx.doi.org/10.21503/cyd.v18i1.1084.

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Se evaluó el efecto de gel formulado a base de extracto hidroalcoholico del fruto de la granada (Punica granatum L.), sobre las hemorroides, en 50 voluntarios de ambos sexos con edades comprendidas entre 20 y 50 años, todos residentes en la ciudad de Lima (Perú). La aplicación y seguimiento farmacológico se realizó dos veces al día durante un mes; la dosis vario según el tamaño de la hemorroides; se excluyó la utilización de cualquier otro preparado convencional. El ensayo, previo consentimiento informado, mostro tener efecto en el proceso de desinflamación del tejido comprometido por las hemorroides. El 46,67% fue evaluado como excelente, el 31,11 % como bueno, el 20,00 % como regular y el 2,22 % como nulo. Se concluye que el fruto de la granada es una fuente de materia prima natural, de bajo costo, que eleva la calidad de atención en enfermería en el tratamiento de las hemorroidesPalabras clave: gel de granada, hemorroides, desinflamación.DOI: http://dx.doi.org/10.21503/CienciayDesarrollo.2015.v18i1.02
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Rojas, Juan P., Sergio G. Ronceros et Olga Palacios. « Evaluación in vitro de la actividad antileishmaniásica del extracto metanólico de siete plantas medicinales ». Ciencia e Investigación 15, no 2 (31 décembre 2012) : 90–95. http://dx.doi.org/10.15381/ci.v15i2.2664.

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El propósito del presente estudio fue determinar la actividad antileishmaniásica in vitro del extracto metanólico de 7 plantas medicinales, y su efecto sobre la producción de óxido nítrico en macrófagos. Los extractos, en concentración de 250 μg/mL, fueron probados contra amastigotes intracelulares. Los macrófagos peritoneales de ratón infectados con amastigotes de Leishmania peruviana fueron coloreados con Giemsa y examinados microscópicamente para determinar la carga parasitaria por 100 macrófagos. El óxido nítrico se determinó indirectamente por su conversión a nitrito. Piper aduncum (matico) redujo la supervivencia de los amastigotes hasta 51,30% (p < 0,001), también se observó efecto reductor con Eucalyptus globulus (eucalipto) y Punica granatum (granado); mientras que Annona cherimola (chirimoya), Annona muricata (guanábana) y Origanum vulgare (orégano) fueron inactivos. La producción de óxido nítrico por los macrófagos fue incrementada significativamente por efecto de Annona cherimola, que produjo la concentración de 13,50 ± 1,50 μM en comparación con 4,90 ± 0,10 μM del control (p < 0,001). También causaron incremento significativo de nitrito Origanum vulgare (p < 0,05) y Punica granatum (p < 0,01). Se concluye que Piper aduncum, Eucalyptus globulus y Punica granatum exhiben moderado efecto antileishmaniásico in vitro, y que la producción de óxido nítrico es uno de los probables mecanismos de acción para Punica granatum.
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Waghulde, Sandeep, Sweety Bhopi, Trunali Ghude, Roshani Gotarane et Mohan Kale. « Comparative Anti-Inflammatory Activity of Aril Extracts of Punica granatum Fruits ». Proceedings 9, no 1 (14 novembre 2018) : 66. http://dx.doi.org/10.3390/ecsoc-22-05783.

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A substanceor treatment with an anti-inflammatory propertyis one that reduces inflammation or swelling. The main objective of this study is to evaluate the anti-inflammatory activity of pomegranate aril extract on rat paw. The anti-inflammatory activity of pomegranate was tested on rats by employing the induced carrageenan rat paw edema method. Various concentrations of the arils and the aril mixture (1:1) were prepared by dissolving in hydroalcohol and alcohol to obtain final concentrations of 100 mg/kg, 200 mg/kg, and 400 mg/kg to betested against the organisms. The effectivity of granatin B inaqueous and alcoholic extracts of the arils of Punica granatum was calculated by measuring the increase in paw volume and the percent of inhibition by comparing with the control and the standard drug.
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S.V., HAJIYEVA. « ASSESSMENT OF BIODIVERSITY OF GRANATE GENOTYPES (P. GRANATUM L.) DISTRIBUTED IN AZERBAIJAN BY SOME SIGNS OF YIELD ». AIC development problems of the region 3, no 43 (juillet 2020) : 32–40. http://dx.doi.org/10.15217/issn2079-0996.2020.3.32.

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Fuentes Segura, Rosalín Viviana, Delia Marily Yovana Montero Núñez et Liz Amelia Juanitaflor Morales Cabrera. « OPTIMIZACIÓN DE UNA CERVEZA TIPO LAGER SABORIZADA CON ZUMO DE GRANADA (Punica granatum L.) ». INGENIERÍA : Ciencia, Tecnología e Innovación 1, no 1 (31 août 2015) : 107. http://dx.doi.org/10.26495/icti.v1i1.111.

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El objetivo principal de esta investigación fue optimizar una cerveza tipo lager saborizada con zumo de granada, como alternativa de empleo y valor agregado a esta materia prima que empieza a posicionarse en el mercado internacional, incorporando el zumo en una bebida tan consumida por el mundo como la cerveza tipo lager. Las variables dependientes evaluación de la aceptabilidad del producto final habiendo estudiado en la formulación la concentración de zumo de granada a incorporar durante la segunda fermentación, y el tiempo de fermentación para obtener los grados alcohólicos reglamentados para una cerveza tipo lager. Se desarrollaron 16 tratamientos generados a partir del software Design Expert 8.0 aplicando una metodología de superficie de respuesta. Una cerveza tipo lager saborizada con zumo de granada requiere de la incorporación del mismo en la fermentación secundaria debiendo permanecer por un periodo de 7-10 días y a una temperatura de -2 a 2 °C. Para cortar la fermentación con meta bisulfito de potasio a 0.15g/l y sorbito de potasio 0.12g/l, luego la separación de sólidos para finalmente pasar a la carbonatación con el 2.5% de CO2. e independientes estudiadas estuvieron asociadas a La formulación óptima fue 23% zumo de granada para lograr 8°Bx, 4pH y 5% de alcohol como características finales. La evaluación sensorial reportan una aceptabilidad de la cerveza tipo lager con: color rojo violáceo, sabor agridulce, aroma agradable, textura líquida. Logrando una aceptación por parte del 52% de los panelistas.
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Maillard Berdeja, Karla Vanessa, Edith Ponce Alquicira et Beatriz Sofía Schettino Bermúdez. « Pomegranate peel (Punica granatum L.) : potential use as a source of functional ingredients in emulsified cooked meat products ». Nacameh 12, no 2 (30 décembre 2018) : 30–41. http://dx.doi.org/10.24275/uam/izt/dcbs/nacameh/2018v12n2/maillard.

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