Littérature scientifique sur le sujet « Formalin-fixed, paraffin-embedded tissues- FFPE »
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Articles de revues sur le sujet "Formalin-fixed, paraffin-embedded tissues- FFPE"
Obi, Ekenedirichukwu N., Daniel A. Tellock, Gabriel J. Thomas et Timothy D. Veenstra. « Biomarker Analysis of Formalin-Fixed Paraffin-Embedded Clinical Tissues Using Proteomics ». Biomolecules 13, no 1 (3 janvier 2023) : 96. http://dx.doi.org/10.3390/biom13010096.
Texte intégralMagdeldin, Sameh, et Tadashi Yamamoto. « Toward deciphering proteomes of formalin-fixed paraffin-embedded (FFPE) tissues ». PROTEOMICS 12, no 7 (avril 2012) : 1045–58. http://dx.doi.org/10.1002/pmic.201100550.
Texte intégralDear, Jonathan D., Jane E. Sykes et Danika L. Bannasch. « Quality of DNA extracted from formalin-fixed, paraffin-embedded canine tissues ». Journal of Veterinary Diagnostic Investigation 32, no 4 (9 juin 2020) : 556–59. http://dx.doi.org/10.1177/1040638720929637.
Texte intégralMichelsen, Nete V., Klaus Brusgaard, Qihua Tan, Mads Thomassen, Khalid Hussain et Henrik T. Christesen. « Investigation of Archived Formalin-Fixed Paraffin-Embedded Pancreatic Tissue with Whole-Genome Gene Expression Microarray ». ISRN Pathology 2011 (26 décembre 2011) : 1–12. http://dx.doi.org/10.5402/2011/275102.
Texte intégralGuo, Tong, Weijie Wang, Paul A. Rudnick, Tao Song, Jie Li, Zhengping Zhuang, Robert J. Weil, Don L. DeVoe, Cheng S. Lee et Brian M. Balgley. « Proteome Analysis of Microdissected Formalin-fixed and Paraffin-embedded Tissue Specimens ». Journal of Histochemistry & ; Cytochemistry 55, no 7 (19 mars 2007) : 763–72. http://dx.doi.org/10.1369/jhc.7a7177.2007.
Texte intégralŞEVİK, Murat. « The extraction of Peste Des Petits Ruminants Virus RNA from paraffin-embedded tissues using a modified extraction method ». Journal of Advances in VetBio Science and Techniques 7, no 2 (31 août 2022) : 202–9. http://dx.doi.org/10.31797/vetbio.1078235.
Texte intégralSequeiros, Tamara, Marta García, Melania Montes, Mireia Oliván, Marina Rigau, Eva Colás, Inés de Torres, Juan Morote, Jaume Reventós et Andreas Doll. « Molecular Markers for Prostate Cancer in Formalin-Fixed Paraffin-Embedded Tissues ». BioMed Research International 2013 (2013) : 1–15. http://dx.doi.org/10.1155/2013/283635.
Texte intégralChung, Joon-Yong, Till Braunschweig, Reginald Williams, Natalie Guerrero, Karl M. Hoffmann, Mijung Kwon, Young K. Song, Steven K. Libutti et Stephen M. Hewitt. « Factors in Tissue Handling and Processing That Impact RNA Obtained From Formalin-fixed, Paraffin-embedded Tissue ». Journal of Histochemistry & ; Cytochemistry 56, no 11 (21 juillet 2008) : 1033–42. http://dx.doi.org/10.1369/jhc.2008.951863.
Texte intégralNucci, Ricardo, Wilson Jacob-Filho, Alexandre Busse, Laura Maifrino et Romeu de Souza. « Anatomopathological Assessment of the Diaphragm in Formalin-Fixed, Paraffin-Embedded Sections ». Journal of Morphological Sciences 35, no 03 (septembre 2018) : 173–76. http://dx.doi.org/10.1055/s-0038-1673611.
Texte intégralHe, Helen, Yu Yang, Zhongmin Xiang, Lunyin Yu, Jouhara Chouitar, Jie Yu, Natalie Roy D’Amore et al. « A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts ». Journal of Biomarkers 2016 (10 mai 2016) : 1–11. http://dx.doi.org/10.1155/2016/1274603.
Texte intégralThèses sur le sujet "Formalin-fixed, paraffin-embedded tissues- FFPE"
Matilda, Rentoft. « The use of formalin fixed paraffin embedded tissue and global gene expression profiling for increased understanding of squamous cell carcinoma of the tongue ». Doctoral thesis, Umeå universitet, Patologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-54005.
Texte intégralRossouw, Sophia Catherine. « Optimisation of proteomics techniques for archival tumour blocks of a South African cohort of colorectal cancer ». University of Western Cape, 2020. http://hdl.handle.net/11394/8036.
Texte intégralTumour-specific protein markers are usually present at elevated concentrations in patient biopsy tissue; therefore tumour tissue is an ideal biological material for studying cancer proteomics and biomarker discovery studies. To understand and elucidate cancer pathogenesis and its mechanisms at the molecular level, the collection and characterisation of a large number of individual patient tissue cohorts are required. Since most pathology institutes routinely preserve biopsy tissues by standardised methods of formalin fixation and paraffin embedment, these archived, FFPE tissues are important collections of pathology material, often accompanied by important metadata, such as patient medical history and treatments. FFPE tissue blocks are conveniently stored under ambient conditions for decades, while retaining cellular morphology due to the modifications induced by formalin.
2022
Djidja, M.-C., S. Francese, Paul M. Loadman, Chris W. Sutton, P. Scriven, E. Claude, M. F. Snel, J. Franck, M. Salzet et M. R. Clench. « Detergent addition to trypsin digest and Ion Mobility Separation prior to MS/MS improves peptide yield and Protein Identification for in situ Proteomic Investigation of Frozen and FFPE Adenocarcinoma tissue sections ». Wiley, 2009. http://hdl.handle.net/10454/4565.
Texte intégralThe identification of proteins involved in tumour progression or which permit enhanced or novel therapeutic targeting is essential for cancer research. Direct MALDI analysis of tissue sections is rapidly demonstrating its potential for protein imaging and profiling in the investigation of a range of disease states including cancer. MALDI-mass spectrometry imaging (MALDI-MSI) has been used here for direct visualisation and in situ characterisation of proteins in breast tumour tissue section samples. Frozen MCF7 breast tumour xenograft and human formalin-fixed paraffin-embedded breast cancer tissue sections were used. An improved protocol for on-tissue trypsin digestion is described incorporating the use of a detergent, which increases the yield of tryptic peptides for both fresh frozen and formalin-fixed paraffin-embedded tumour tissue sections. A novel approach combining MALDI-MSI and ion mobility separation MALDI-tandem mass spectrometry imaging for improving the detection of low-abundance proteins that are difficult to detect by direct MALDI-MSI analysis is described. In situ protein identification was carried out directly from the tissue section by MALDI-MSI. Numerous protein signals were detected and some proteins including histone H3, H4 and Grp75 that were abundant in the tumour region were identified
Clift, Sarah J. « Standardization and validation of an immunoperoxidase test for African horsesickness virus using formalin-fixed, paraffin-embedded tissues ». Diss., University of Pretoria, 2009. http://hdl.handle.net/2263/24626.
Texte intégralDissertation (MSc)--University of Pretoria, 2008.
Paraclinical Sciences
unrestricted
Clift, Sarah Jane. « Standardization and validation of an immunoperoxidase test for African horsesickness virus using formalin-fixed, paraffin-embedded tissues ». Pretoria : [s.n.], 2008. http://upetd.up.ac.za/thesis/available/etd-05132009-173308/.
Texte intégralViljoen, Rabia. « Optimisation of sample preparation for DNA extraction from formalin fixed paraffin embedded tissues of unresolved sudden unexpected death cases ». Master's thesis, Faculty of Health Sciences, 2021. http://hdl.handle.net/11427/33072.
Texte intégralHutamo, Kutlwano Aggrineth. « Typing of Mycobacterium bovis in formalin-fixed, paraffin-embedded tissues from selected wildlife species in the Kruger National Park, South Africa ». Diss., University of Pretoria, 2012. http://hdl.handle.net/2263/29674.
Texte intégralDissertation (MSc)--University of Pretoria, 2012.
Veterinary Tropical Diseases
unrestricted
Lüder, Ripoli Florenza [Verfasser]. « Comparison of fresh frozen vs. formalin-fixed, paraffin-embedded specimens and the expression profiling of 16 target genes in neoplastic and non-neoplastic canine mammary tissues using a multiplex branched-DNA assay / Florenza Lüder Ripoli ». Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2016. http://d-nb.info/1125394560/34.
Texte intégralKuo, Shanny Hsuan, et 郭. 軒. « Molecular Detection of Feline Coronaviruses in Formalin-Fixed and Paraffin-Embedded Tissue (FFPE) by nested RT-PCRs : a Diagnosis-Aiding Approach ». Thesis, 2017. http://ndltd.ncl.edu.tw/handle/dc943h.
Texte intégral國立臺灣大學
分子暨比較病理生物學研究所
105
Feline infectious peritonitis (FIP), caused by feline coronavirus (FCoV), is a lethal disease in cats. The clinical signs are non-specific and antemortem diagnosis remains challenging and frustrating. Appling histopathology combined with immunohistochemical (IHC) staining is considered as the gold standard for FIP diagnosis. However, the sensitivity of the IHC method depends much on the numbers of intralesional antigen-bearing cells. Due to the limitations of small sampling sizes as well as the equivocal IHC staining pattern in some specimens, formalin-fixed and paraffin-embedded tissue (FFPE) biopsies frequently submitted for histopathological examination for FIP are the most challenging specimens for pathologists. It has been demonstrated that the consensus PCR targeting 3’UTR alone is non-specific for diagnosis of FIP in fresh tissues. Moreover, two recently described mutations, the substitution of methionine (M) to leucine (L) amino acid mutation at position 1058 (M1058L) and the substitution of serine (S) to alanine (A) amino acid mutation at position 1060 (S1060A) in spike (S) gene, which together can distinguish feline infectious peritonitis virus (FIPV) from feline enteric coronavirus (FECV) in >95% of serotype I FCoV-infected cases in freshly-collected specimens, have suggested a potential diagnostic value. The aim of this study was to compare the uses of a consensus nested RT-PCR (nRT-PCR) targeting 3’UTR and a nRT-PCR targeting the two mutations in S gene in aiding the diagnosis of FIP in FFPE tissues. After evaluation of the RNA quality in FFPE tissues by a RT-PCR targeting the housekeeping gene of feline GAPDH, a total of 38 histopathologically and immunohistochemically confirmed FIP cases and 22 non-FIP cases were used as the source of RNA and examined nRT-PCRs. We have successfully extracted RNA and amplified FCoV genes in 31/38 (82%) FIP cases using consensus nRT-PCR, whereas 17/38 (42%) FIP cases were detected using the S-specific nRT-PCR. Following subsequent sequencing, 16 out of 17 serotype 1 cases had one of the two mutations (M1058L and S1060A) in the S gene. None of the FFPF tissues from these non-FIP cats were positive by both methods. We have demonstrated that in combined with histopathology and IHC staining, both consensus nRT-PCR and S-specific nRT-PCR were capable of detecting viral RNA from FFPE samples where IHC signals were equivocal and possibly misinterpreted as negativity. Both methods serve as a useful tool in supporting FIP diagnosis and for the retrospective study of FIP in archival FFPE tissues.
Zhang, XIAO. « EVALUATION OF RNA QUALITY FROM FORMALIN FIXED AND PARAFFIN EMBEDDED SAMPLES:APPLICATIONS AND LIMITATIONS ». Thesis, 2008. http://hdl.handle.net/1974/1740.
Texte intégralThesis (Master, Pathology & Molecular Medicine) -- Queen's University, 2008-09-26 10:49:50.044
Livres sur le sujet "Formalin-fixed, paraffin-embedded tissues- FFPE"
Al-Mulla, Fahd, dir. Formalin-Fixed Paraffin-Embedded Tissues. Totowa, NJ : Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-055-3.
Texte intégralFormalin-fixed paraffin-embedded tissues : Methods and protocols. New York : Humana Press, 2011.
Trouver le texte intégralFormalin-Fixed Paraffin-Embedded Tissues : Methods and Protocols. Humana Press, 2016.
Trouver le texte intégralChapitres de livres sur le sujet "Formalin-fixed, paraffin-embedded tissues- FFPE"
Bonin, Serena, Patricia J. T. A. Groenen, Iris Halbwedl et Helmut H. Popper. « DNA Extraction from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues ». Dans Guidelines for Molecular Analysis in Archive Tissues, 33–36. Berlin, Heidelberg : Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-17890-0_7.
Texte intégralHowe, Karen. « Extraction of miRNAs from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues ». Dans Methods in Molecular Biology, 17–24. New York, NY : Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6524-3_3.
Texte intégralLonguespée, Rémi, Dominique Baiwir, Gabriel Mazzucchelli, Nicolas Smargiasso et Edwin De Pauw. « Laser Microdissection-Based Microproteomics of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues ». Dans Methods in Molecular Biology, 19–31. New York, NY : Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7558-7_2.
Texte intégralPiqueras, Marta, Manish Mani Subramaniam, Samuel Navarro, Nina Gale et Rosa Noguera. « Fluorescence In Situ Hybridization (FISH) on Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Sections ». Dans Guidelines for Molecular Analysis in Archive Tissues, 225–30. Berlin, Heidelberg : Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-17890-0_34.
Texte intégralTran, Diem, Mark Daniels, Ben Colson, Dikran Aivazian, Antonio Boccia, Ingrid Joseph, Steffan Ho, Steve French, Alex Buko et Jing Wei. « Sample Preparation of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue for Proteomic Analyses ». Dans Sample Preparation in Biological Mass Spectrometry, 159–70. Dordrecht : Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-0828-0_10.
Texte intégralAzimzadeh, Omid, Michael J. Atkinson et Soile Tapio. « Quantitative Proteomic Analysis Using Formalin-Fixed, Paraffin-Embedded (FFPE) Human Cardiac Tissue ». Dans Methods in Molecular Biology, 525–33. New York, NY : Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1186-9_33.
Texte intégralAzimzadeh, Omid, Michael J. Atkinson et Soile Tapio. « Qualitative and Quantitative Proteomic Analysis of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue ». Dans Methods in Molecular Biology, 109–15. New York, NY : Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2550-6_10.
Texte intégralBonin, Serena, Patricia J. T. A. Groenen, Iris Halbwedl et Helmut H. Popper. « DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues (FFPE) (from Small Fragments of Tissues or Microdissected Cells) ». Dans Guidelines for Molecular Analysis in Archive Tissues, 37–39. Berlin, Heidelberg : Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-17890-0_8.
Texte intégralGomes, Bruno Costa, Bruno Santos, José Rueff et António Sebastião Rodrigues. « Methods for Studying MicroRNA Expression and Their Targets in Formalin-Fixed, Paraffin-Embedded (FFPE) Breast Cancer Tissues ». Dans Methods in Molecular Biology, 189–205. New York, NY : Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3347-1_11.
Texte intégralDowling, Paul. « DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue ». Dans Methods in Molecular Biology, 113–18. New York, NY : Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2831-7_9.
Texte intégralActes de conférences sur le sujet "Formalin-fixed, paraffin-embedded tissues- FFPE"
Galindo, Hector, Luisa Solis, Junya Fujimoto, Nana E. Hanson, Christina McDowell, Erick Riquelme, XiMing Tang et al. « Abstract 3195 : Formalin-fixed and paraffin-embedded (FFPE) DNA recovery for high-throughput genotyping of lung cancer tissues ». Dans Proceedings : AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012 ; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-3195.
Texte intégralReinholz, Monica M., Debrah M. Thompson, Ihab Botros, Matt Rounseville et Patrick C. Roche. « Abstract 1383 : NGS-based measurement of gene expression of 2560 oncology-related biomarkers in formalin-fixed, paraffin-embedded (FFPE) tissues ». Dans Proceedings : AACR 107th Annual Meeting 2016 ; April 16-20, 2016 ; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-1383.
Texte intégralPalescandolo, Emanuele, Robert Jones, Alina Raza, Ashwini Sunkavalli, Priscilla K. Brastianos, Matthew Ducar, Christina Go et al. « Abstract 3178 : Can DNA from archived formalin-fixed paraffin embedded (FFPE) cancer tissues be used for somatic mutation analysis in next generation sequencing ». Dans Proceedings : AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012 ; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-3178.
Texte intégralEnglert, D., D. Wilson et S. Laken. « Amplification of mRNA from Formalin-Fixed Paraffin-Embedded (FFPE) Breast Cancer Tissues without 3' Bias and Multiplex Expression Analysis on Flow-Through Microarrays. » Dans Abstracts : Thirty-Second Annual CTRC‐AACR San Antonio Breast Cancer Symposium‐‐ Dec 10‐13, 2009 ; San Antonio, TX. American Association for Cancer Research, 2009. http://dx.doi.org/10.1158/0008-5472.sabcs-09-3052.
Texte intégralGu, Jian. « Abstract 4127 : Expression profiling of paired formalin-fixed, paraffin-embedded (FFPE) and fresh-frozen tissue samples on Ion Torrent PGMTM. » Dans Proceedings : AACR 104th Annual Meeting 2013 ; Apr 6-10, 2013 ; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-4127.
Texte intégralSmiley, Sheila, Celia Marginean et Bryan Lo. « Abstract A40 : Gene expression profiling of pancreatic cancer precursors directly from formalin fixed paraffin embedded (FFPE) tissue without nucleic acid extraction ». Dans Abstracts : AACR Special Conference on Pancreatic Cancer : Advances in Science and Clinical Care ; May 12-15, 2016 ; Orlando, FL. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.panca16-a40.
Texte intégralThobe, Megan N., Suzane L. Um, Victoria L. Peek, Darryl W. Ballard, Bruce W. Konicek, Kelly M. Credille, Philip J. Ebert, Gregory P. Donoho et S. Betty Yan. « Abstract 2087 : Detection of KRAS mutations in circulating tumor cells (CTCs) and in formalin-fixed, paraffin-embedded (FFPE) tissue using castPCR method ». Dans Proceedings : AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012 ; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-2087.
Texte intégralBaumbach, L., J. Yan, M. Ahearn, C. Gomez, M. Jorda, T. Halsey, A. Mejias et al. « Gene Expression Profiling of Formalin-Fixed, Pariffin-Embedded (FFPE) Tissues from Triple-Negative (TN) Breast Cancer (BC) Patients. » Dans Abstracts : Thirty-Second Annual CTRC‐AACR San Antonio Breast Cancer Symposium‐‐ Dec 10‐13, 2009 ; San Antonio, TX. American Association for Cancer Research, 2009. http://dx.doi.org/10.1158/0008-5472.sabcs-09-6125.
Texte intégralNiland, Erin, Audrey D. McGuire, Mary L. Cox et George E. Sandusky. « Abstract 3203 : High quality DNA obtained with an automated DNA Extraction method with 15 to 40 year old formalin fixed paraffin embedded (FFPE) blocks from normal and cancer tissues ». Dans Proceedings : AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012 ; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-3203.
Texte intégralRusbuldt, Joshua James, Tanesha Cash-Mason, Shaozhou Tian, Alison VanSchoiack, Yan Liang, Chandra Rao et Denis Smirnov. « Abstract 4691 : Evaluation of the NanoString’s Digital Spatial Profiling (DSP) technology in formalin-fixed paraffin embedded (FFPE) cell line mixtures, PBMCs and non-small cell lung cancer (NSCLC) tissues ». Dans Proceedings : AACR Annual Meeting 2019 ; March 29-April 3, 2019 ; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-4691.
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