Articles de revues : « Fmoc-FF »

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Gallo, Enrico, Carlo Diaferia, Sabrina Giordano, Elisabetta Rosa, Barbara Carrese, Gennaro Piccialli, Nicola Borbone, Giancarlo Morelli, Giorgia Oliviero et Antonella Accardo. « Ultrashort Cationic Peptide Fmoc-FFK as Hydrogel Building Block for Potential Biomedical Applications ». Gels 10, n^o 1 (22 décembre 2023) : 12. http://dx.doi.org/10.3390/gels10010012.

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Fmoc-diphenylalanine (Fmoc-FF) is a low-molecular-weight peptide hydrogelator. This simple all-aromatic peptide can generate self-supporting hydrogel materials, which have been proposed as novel materials for diagnostic and pharmaceutical applications. Our knowledge of the molecular determinants of Fmoc-FF aggregation is used as a guide to design new peptide-based gelators, with features for the development of improved tools. Here, we enlarge the plethora of Fmoc-FF-based hydrogelated matrices by studying the properties of the Fmoc-FFK tripeptide, alone or in combination with Fmoc-FF. For multicomponent matrices, the relative weight ratios between Fmoc-FFK and Fmoc-FF (specifically, 1/1, 1/5, 1/10, and 1/20 w/w) are evaluated. All the systems and their multiscale organization are studied using different experimental techniques, including rheology, circular dichroism, Fourier transform infrared spectroscopy, and scanning electron microscopy (SEM). Preliminary profiles of biocompatibility for the studied systems are also described by testing them in vitro on HaCaT and 3T3-L1 cell lines. Additionally, the lysine (K) residue at the C-terminus of the Fmoc-FF moiety introduces into the supramolecular material chemical functions (amino groups) which may be useful for modification/derivatization with bioactive molecules of interest, including diagnostic probes, chelating agents, active pharmaceutical ingredients, or peptide nucleic acids.

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Choe, Ranjoo, et Seok Il Yun. « Fmoc-diphenylalanine-based hydrogels as a potential carrier for drug delivery ». e-Polymers 20, n^o 1 (24 août 2020) : 458–68. http://dx.doi.org/10.1515/epoly-2020-0050.

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AbstractSelf-assembled hydrogels from 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) peptides were evaluated as potential vehicles for drug delivery. During self-assembly of Fmoc-FF, high concentrations of indomethacin (IDM) drugs were shown to be incorporated into the hydrogels. The β-sheet arrangement of peptides was found to be predominant in Fmoc-FF–IDM hydrogels regardless of the IDM content. The release mechanism for IDM displayed a biphasic profile comprising an initial hydrogel erosion-dominated stage followed by the diffusion-controlled stage. Small amounts of polyamidoamine dendrimer (PAMAM) added to the hydrogel (Fmoc-FF 0.5%–IDM 0.5%–PAMAM 0.03%) resulted in a more prolonged IDM release compared with Fmoc-FF 0.5%–IDM 0.5% hydrogel. Furthermore, these IDM-loaded hydrogels demonstrated excellent thixotropic response and injectability, which make them suitable candidates for use as injectable self-healing matrices for drug delivery.

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Diaferia, Carlo, Giancarlo Morelli et Antonella Accardo. « Fmoc-diphenylalanine as a suitable building block for the preparation of hybrid materials and their potential applications ». Journal of Materials Chemistry B 7, n^o 34 (2019) : 5142–55. http://dx.doi.org/10.1039/c9tb01043b.

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Due to its capability to self-assemble in self-supporting hydrogels (HG) under physiological conditions, Fmoc-FF is one of the most studied ultra-short peptide. This feature pushed towards the development of novel Fmoc-FF multicomponent systems.

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Mayans, Enric, et Carlos Alemán. « Revisiting the Self-Assembly of Highly Aromatic Phenylalanine Homopeptides ». Molecules 25, n^o 24 (20 décembre 2020) : 6037. http://dx.doi.org/10.3390/molecules25246037.

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Diphenylalanine peptide (FF), which self-assembles into rigid tubular nanostructures, is a very short core recognition motif in Alzheimer’s disease β-amyloid (Aβ) polypeptide. Moreover, the ability of the phenylalanine (F or Phe)-homopeptides to self-assemble into ordered nanostructures has been proved. Within this context it was shown that the assembly preferences of this family of compounds is altered by capping both the N- and C-termini using highly aromatic fluorenyl groups (i.e., fluorenyl-9-methoxycarbonyl and 9-fluorenylmethyl ester, named Fmoc and OFm, respectively). In this article the work performed in the field of the effect of the structure and incubation conditions on the morphology and polymorphism of short (from two to four amino acid residues) Phe-homopeptides is reviewed and accompanied by introducing some new results for completing the comparison. Special attention has been paid to the influence of solvent: co-solvent mixture used to solubilize the peptide, the peptide concentration and, in some cases, the temperature. More specifically, uncapped (FF, FFF, and FFFF), N-capped with Fmoc (Fmoc-FF, Fmoc-FFF, and Fmoc-FFFF), C-capped with OFm (FF-OFm), and doubly capped (Fmoc-FF-OFm, Fmoc-FFF-OFm, and Fmoc-FFFF-OFm) Phe-homopeptides have been re-measured. Although many of the experienced assembly conditions have been only revisited as they were previously reported, other experimental conditions have been examined by the first time in this work. In any case, pooling the effect of highly aromatic blocking groups in a single study, using a wide variety of experimental conditions, allows a perspective of how the disappearance of head-to-tail electrostatic interactions and the gradual increase in the amount of π–π stacking interactions, affects the morphology of the assemblies. Future technological applications of Phe-homopeptides can be envisaged by choosing the most appropriate self-assemble structure, defining not only the length of the peptide but also the amount and the position of fluorenyl capping groups.

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Smaldone, Giovanni, Elisabetta Rosa, Enrico Gallo, Carlo Diaferia, Giancarlo Morelli, Mariano Stornaiuolo et Antonella Accardo. « Caveolin-Mediated Internalization of Fmoc-FF Nanogels in Breast Cancer Cell Lines ». Pharmaceutics 15, n^o 3 (22 mars 2023) : 1026. http://dx.doi.org/10.3390/pharmaceutics15031026.

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Introduction: Hydrogel nanoparticles, also known as nanogels (NGs), have been recently proposed as alternative supramolecular vehicles for the delivery of biologically relevant molecules like anticancer drugs and contrast agents. The inner compartment of peptide based NGs can be opportunely modified according to the chemical features of the cargo, thus improving its loading and release. A full understanding of the intracellular mechanism involved in nanogel uptake by cancer cells and tissues would further contribute to the potential diagnostic and clinical applications of these nanocarriers, allowing the fine tuning of their selectivity, potency, and activity. The structural characterization of nanogels were assessed by Dynamic Light Scattering (DLS) and Nanoparticles Tracking Analysis (NTA) analysis. Cells viability of Fmoc-FF nanogels was evaluated by MTT assay on six breast cancer cell lines at different incubation times (24, 48, and 72 h) and peptide concentrations (in the range 6.25 × 10−4 ÷ 5·10−3 × wt%). The cell cycle and mechanisms involved in Fmoc-FF nanogels intracellular uptake were evaluated using flow cytometry and confocal analysis, respectively. Fmoc-FF nanogels, endowed with a diameter of ~130 nm and a zeta potential of ~−20.0/−25.0 mV, enter cancer cells via caveolae, mostly those responsible for albumin uptake. The specificity of the machinery used by Fmoc-FF nanogels confers a selectivity toward cancer cell lines overexpressing the protein caveolin1 and efficiently performing caveolae-mediated endocytosis.

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Giordano, Sabrina, Enrico Gallo, Carlo Diaferia, Elisabetta Rosa, Barbara Carrese, Nicola Borbone, Pasqualina Liana Scognamiglio, Monica Franzese, Giorgia Oliviero et Antonella Accardo. « Multicomponent Peptide-Based Hydrogels Containing Chemical Functional Groups as Innovative Platforms for Biotechnological Applications ». Gels 9, n^o 11 (15 novembre 2023) : 903. http://dx.doi.org/10.3390/gels9110903.

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Multicomponent hydrogels (HGs) based on ultrashort aromatic peptides have been exploited as biocompatible matrices for tissue engineering applications, the delivery of therapeutic and diagnostic agents, and the development of biosensors. Due to its capability to gel under physiological conditions of pH and ionic strength, the low molecular-weight Fmoc-FF (Nα-fluorenylmethoxycarbonyl-diphenylalanine) homodimer is one of the most studied hydrogelators. The introduction into the Fmoc-FF hydrogel of additional molecules like protein, organic compounds, or other peptide sequences often allows the generation of novel hydrogels with improved mechanical and functional properties. In this perspective, here we studied a library of novel multicomponent Fmoc-FF based hydrogels doped with different amounts of the tripeptide Fmoc-FFX (in which X= Cys, Ser, or Thr). The insertion of these tripeptides allows to obtain hydrogels functionalized with thiol or alcohol groups that can be used for their chemical post-derivatization with bioactive molecules of interest like diagnostic or biosensing agents. These novel multicomponent hydrogels share a similar peptide organization in their supramolecular matrix. The hydrogels’ biocompatibility, and their propensity to support adhesion, proliferation, and even cell differentiation, assessed in vitro on fibroblast cell lines, allows us to conclude that the hybrid hydrogels are not toxic and can potentially act as a scaffold and support for cell culture growth.

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Dudukovic, Nikola A., et Charles F. Zukoski. « Gelation of Fmoc-diphenylalanine is a first order phase transition ». Soft Matter 11, n^o 38 (2015) : 7663–73. http://dx.doi.org/10.1039/c5sm01399b.

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Ariawan, A. Daryl, Biyun Sun, Jonathan P. Wojciechowski, Ian Lin, Eric Y. Du, Sophia C. Goodchild, Charles G. Cranfield, Lars M. Ittner, Pall Thordarson et Adam D. Martin. « Effect of polar amino acid incorporation on Fmoc-diphenylalanine-based tetrapeptides ». Soft Matter 16, n^o 20 (2020) : 4800–4805. http://dx.doi.org/10.1039/d0sm00320d.

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The incorporation of polar amino acids into the Fmoc-FF motif yields tetrapeptide hydrogels whose biocompatibility in the gel state is inversely proportional to their biocompatibility in the solution state.

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Diaferia, Carlo, Elisabetta Rosa, Giancarlo Morelli et Antonella Accardo. « Fmoc-Diphenylalanine Hydrogels : Optimization of Preparation Methods and Structural Insights ». Pharmaceuticals 15, n^o 9 (25 août 2022) : 1048. http://dx.doi.org/10.3390/ph15091048.

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Hydrogels (HGs) are tri-dimensional materials with a non-Newtonian flow behaviour formed by networks able to encapsulate high amounts of water or other biological fluids. They can be prepared using both synthetic or natural polymers and their mechanical and functional properties may change according to the preparation method, the solvent, the pH, and to others experimental parameters. Recently, many short and ultra-short peptides have been investigated as building blocks for the formulation of biocompatible hydrogels suitable for different biomedical applications. Due to its simplicity and capability to gel in physiological conditions, Fmoc-FF dipeptide is one of the most studied peptide hydrogelators. Although its identification dates to 15 ago, its behaviour is currently studied because of the observation that the final material obtained is deeply dependent on the preparation method. To collect information about their formulation, here are reported some different strategies adopted until now for the Fmoc-FF HG preparation, noting the changes in the structural arrangement and behaviour in terms of stiffness, matrix porosity, and stability induced by the different formulation strategy on the final material.

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Luo, Xin, Boya Ding et Xingcen Liu. « Poly(acrylic acid)/Dipeptide Double-Network Hydrogel to Achieve a Highly Stretchable Strain Sensor ». Chemosensors 10, n^o 9 (9 septembre 2022) : 360. http://dx.doi.org/10.3390/chemosensors10090360.

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Flexible and stretchable strain sensors can be applied for human health monitoring and disease diagnoses via the output of multiple biophysical signals. However, it is still a challenge to fabricate short-peptide-based strain sensors. Here, we prepared a novel polymer-dipeptide double-network hydrogel with excellent stretchability, responsiveness, and stability. The poly(acrylic acid) (PAA) gel, by cross-linking, maintains mechanical and flexible properties, and the fluorenyl methoxycarbonyl-diphenylalanine (Fmoc-FF) network, by non-covalent interactions, is helpful for energy dissipation. With increasing tensile or compression strains, the PAA/Fmoc-FF hydrogel exhibited a high mechanical strength and fast recovery. Moreover, as the presence of KCl improves the electronic conductivity, the hybrid gel exhibited a cyclic strain-stress performance, which is the foundation of a strain sensor. Based on that, its application as a motion sensor was demonstrated by monitoring the movements of human joints, such as the forefinger, wrist, elbow, and knee. Consequently, the hybrid polymer-peptide gel could be an ideal candidate for wearable sensors in the future.

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Diaferia, Carlo, Elisabetta Rosa, Enrico Gallo, Giovanni Smaldone, Mariano Stornaiuolo, Giancarlo Morelli et Antonella Accardo. « Self-Supporting Hydrogels Based on Fmoc-Derivatized Cationic Hexapeptides for Potential Biomedical Applications ». Biomedicines 9, n^o 6 (15 juin 2021) : 678. http://dx.doi.org/10.3390/biomedicines9060678.

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Peptide-based hydrogels (PHGs) are biocompatible materials suitable for biological, biomedical, and biotechnological applications, such as drug delivery and diagnostic tools for imaging. Recently, a novel class of synthetic hydrogel-forming amphiphilic cationic peptides (referred to as series K), containing an aliphatic region and a Lys residue, was proposed as a scaffold for bioprinting applications. Here, we report the synthesis of six analogues of the series K, in which the acetyl group at the N-terminus is replaced by aromatic portions, such as the Fmoc protecting group or the Fmoc-FF hydrogelator. The tendency of all peptides to self-assemble and to gel in aqueous solution was investigated using a set of biophysical techniques. The structural characterization pointed out that only the Fmoc-derivatives of series K keep their capability to gel. Among them, Fmoc-K3 hydrogel, which is the more rigid one (G’ = 2526 Pa), acts as potential material for tissue engineering, fully supporting cell adhesion, survival, and duplication. These results describe a gelification process, allowed only by the correct balancing among aggregation forces within the peptide sequences (e.g., van der Waals, hydrogen bonding, and π–π stacking).

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Diaferia, Carlo, Moumita Ghosh, Teresa Sibillano, Enrico Gallo, Mariano Stornaiuolo, Cinzia Giannini, Giancarlo Morelli, Lihi Adler-Abramovich et Antonella Accardo. « Fmoc-FF and hexapeptide-based multicomponent hydrogels as scaffold materials ». Soft Matter 15, n^o 3 (2019) : 487–96. http://dx.doi.org/10.1039/c8sm02366b.

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Rosa, Elisabetta, Enrico Gallo, Teresa Sibillano, Cinzia Giannini, Serena Rizzuti, Eliana Gianolio, Pasqualina Liana Scognamiglio, Giancarlo Morelli, Antonella Accardo et Carlo Diaferia. « Incorporation of PEG Diacrylates (PEGDA) Generates Hybrid Fmoc-FF Hydrogel Matrices ». Gels 8, n^o 12 (16 décembre 2022) : 831. http://dx.doi.org/10.3390/gels8120831.

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Generated by a hierarchical and multiscale self-assembling phenomenon, peptide-based hydrogels (HGs) are soft materials useful for a variety of applications. Short and ultra-short peptides are intriguing building blocks for hydrogel fabrication. These matrices can also be obtained by mixing low-molecular-weight peptides with other chemical entities (e.g., polymers, other peptides). The combination of two or more constituents opens the door to the development of hybrid systems with tunable mechanical properties and unexpected biofunctionalities or morphologies. For this scope, the formulation, the multiscale analysis, and the supramolecular characterization of novel hybrid peptide-polymer hydrogels are herein described. The proposed matrices contain the Fmoc-FF (Nα-fluorenylmethyloxycarbonyl diphenylalanine) hydrogelator at a concentration of 0.5 wt% (5.0 mg/mL) and a diacrylate α-/ω-substituted polyethylene-glycol derivative (PEGDA). Two PEGDA derivatives, PEGDA 1 and PEGDA2 (mean molecular weights of 575 and 250 Da, respectively), are mixed with Fmoc-FF at different ratios (Fmoc-FF/PEGDA at 1/1, 1/2, 1/5, 1/10 mol/mol). All the multicomponent hybrid peptide-polymer hydrogels are scrutinized with a large panel of analytical techniques (including proton relaxometry, FTIR, WAXS, rheometry, and scanning electronic microscopy). The matrices were found to be able to generate mechanical responses in the 2–8 kPa range, producing a panel of tunable materials with the same chemical composition. The release of a model drug (Naphthol Yellow S) is reported too. The tunable features, the different topologies, and the versatility of the proposed materials open the door to the development of tools for different applicative areas, including diagnostics, liquid biopsies and responsive materials. The incorporation of a diacrylate function also suggests the possible development of interpenetrating networks upon cross-linking reactions. All the collected data allow a mutual comparison between the different matrices, thus confirming the significance of the hybrid peptide/polymer-based methodology as a strategy for the design of innovative materials.

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Ryan, Kate, Jason Beirne, Gareth Redmond, Jason I. Kilpatrick, Jill Guyonnet, Nicolae-Viorel Buchete, Andrei L. Kholkin et Brian J. Rodriguez. « Nanoscale Piezoelectric Properties of Self-Assembled Fmoc–FF Peptide Fibrous Networks ». ACS Applied Materials & ; Interfaces 7, n^o 23 (3 juin 2015) : 12702–7. http://dx.doi.org/10.1021/acsami.5b01251.

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Gong, Xiao, Christopher Branford-White, Lei Tao, Shubai Li, Jing Quan, Huali Nie et Limin Zhu. « Preparation and characterization of a novel sodium alginate incorporated self-assembled Fmoc-FF composite hydrogel ». Materials Science and Engineering : C 58 (janvier 2016) : 478–86. http://dx.doi.org/10.1016/j.msec.2015.08.059.

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Orocz, Marta, Kinga Hartman, Marek Smoluch, Jerzy Silberring et Przemyslaw Mielczarek. « Fmoc solid-phase synthesis of RF9 optimization with mass spectrometry verification ». Current Issues in Pharmacy and Medical Sciences 35, n^o 1 (1 juillet 2022) : 11–15. http://dx.doi.org/10.2478/cipms-2022-0003.

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Abstract The RF9 compound, which is an antagonist of the FF neuropeptide receptors is used as a therapeutic substance to improve the effectiveness of opioids in the chronic treatment of pain. The purpose of this study was to find the most efficient method of RF9 synthesis. The optimization experiment involved solid-phase peptide synthesis. The Fmoc strategy is based on the usage of the 9-fluorenylmethoxycarbonyl group to block reactive amino groups. Commonly applied RF9 synthesis is based on DIC/HOBt activation of 1-adamantanecarboxylic acid prior to its substitution. The experiments carried out in this research were based on the routinely applied DIC/HOBt carboxylic group activation and this scheme was compared with the COMU/DIPEA and DIC approach. The obtained results showed that COMU/DIPEA was the most efficient and effective method of RF9 synthesis. Using this strategy, pure compound was obtained, without any by-products, and at a highest yield. The use of COMU/DIPEA can be an excellent alternative to the routinely used RF9 synthesis.

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MacPherson, Douglas, Yaron Bram, Jiwoon Park et Robert E. Schwartz. « Peptide-based scaffolds for the culture and maintenance of primary human hepatocytes ». Scientific Reports 11, n^o 1 (24 mars 2021). http://dx.doi.org/10.1038/s41598-021-86016-5.

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AbstractWe report here the use of a nanofibrous hydrogel as a 3D scaffold for the culture and maintenance of functional primary human hepatocytes. The system is based on the cooperative assembly of a fiber-forming peptide component, fluorenylmethyloxycarbonyl-diphenylalanine (Fmoc-FF), and the integrin-binding functional peptide ligand, Fmoc-arginine-glycine-aspartic acid (Fmoc-RGD) into a nanofibrous gel at physiological pH. This Fmoc-FF/RGD hydrogel was formulated to provide a biomimetic microenvironment with some critical features such as mechanical properties and nanofiber morphology, which were optimized to support hepatocyte culture. The material was shown to support maintenance and function of encapsulated primary human hepatocytes as indicated by actin staining, qRT-PCR, and functional cytochrome P450 assays. The designed gel was shown to outperform Matrigel in cytochrome P450 functional assays. The hydrogel may prove useful for liver development and disease models, as well as providing insights into the design of future implantable scaffolds for the regeneration of liver tissue in patients with liver disease.

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Diaferia, Carlo, Elisabetta Rosa, Enrico Gallo, Giancarlo Morelli et Antonella Accardo. « Differently N‐Capped Analogues of Fmoc‐FF. » Chemistry – A European Journal, 6 mars 2023. http://dx.doi.org/10.1002/chem.202300661.

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Wang, Yunxiao, Qiang Geng, Hao Lyu, Wuxuepeng Sun, Xinyuan Fan, Kang Ma, Kai Wu et al. « Bioinspired Flexible Hydrogelation with Programmable Properties for Tactile Sensing ». Advanced Materials, 28 avril 2024. http://dx.doi.org/10.1002/adma.202401678.

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AbstractTactile sensing requires integrated detection platforms with distributed and highly‐sensitive haptic sensing capabilities along with biocompatibility, aiming to replicate the physiological functions of the human skin and empower industrial robotic and prosthetic wearers to detect tactile information. In this regard, short peptide‐based self‐assembled hydrogels show promising potential to act as bioinspired supramolecular substrates for developing tactile sensors showing biocompatibility and biodegradability. However, the intrinsic difficulty to modulate the mechanical properties severely restricts their extensive employment. Herein, by controlling the self‐assembly of 9‐fluorenylmethoxycarbonyl‐modifid diphenylalanine (Fmoc‐FF) through introduction of polyethylene glycol diacrylate (PEGDA), wider nanoribbons are achieved by untwisting from well‐established thinner nanofibers, and the mechanical properties of the supramolecular hydrogels can be enhanced 10‐fold, supplying bioinspired supramolecular encapsulating substrate for tactile sensing. Furthermore, by doping with PEDOT:PSS and 9‐fluorenylmethoxycarbonyl‐modifid 3,4‐dihydroxy‐L‐phenylalanine (Fmoc‐DOPA), the Fmoc‐FF self‐assembled hydrogels can be engineered to be conductive and adhesive, providing bioinspired sensing units and adhesive layer for tactile sensing applications. Therefore, the integration of these modules results in peptide hydrogelation‐based tactile sensors, showing high sensitivity and sustainable responses with intrinsic biocompatibility and biodegradability. Our findings establish the feasibility of developing programmable peptide self‐assembly with adjustable features for tactile sensing applications.This article is protected by copyright. All rights reserved

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Tongshun, DING, FENG Ping, SUN Xuewen, SHAN Husheng, LI Qi et SONG Jian. « Perovskite Film Passivated by Fmoc-FF-OH and Its Photovoltaic Performance ». Journal of Inorganic Materials, 2023, 50. http://dx.doi.org/10.15541/jim20230050.

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Najafi, Haniyeh, Samira Sadat Abolmaali, Reza Heidari, Hadi Valizadeh, Ali Mohammad Tamaddon et Negar Azarpira. « Integrin receptor-binding nanofibrous peptide hydrogel for combined mesenchymal stem cell therapy and nitric oxide delivery in renal ischemia/reperfusion injury ». Stem Cell Research & ; Therapy 13, n^o 1 (26 juillet 2022). http://dx.doi.org/10.1186/s13287-022-03045-1.

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Abstract Background Mesenchymal-based therapy has been utilized as a practical approach in the treatment of renal ischemia/reperfusion (I/R) injury. However, low cell retention and survival in the ischemic site have remained challenging issues. To bridge this gap, the integrin receptor-binding RGD peptide-functionalized, s-nitroso-n-acetyl penicillamine (SNAP)-loaded hydrogel was used to transplant Wharton's jelly-mesenchymal stem cells (WJ-MSCs). Methods Apart from physicochemical and rheological characterizations that confirmed entangled interlocking β-sheets with nanofibrous morphology, real-time RT-PCR, ROS production, serum biomarker concentrations, and histopathological alterations were explored in a mouse model to assess the therapeutic efficacy of formulations in the treatment of renal I/R injury. Results The RGD-functionalized Fmoc-diphenylalanine (Fmoc-FF + Fmoc-RGD) hydrogel supported the spread and proliferation of WJ-MSCs in vivo. Notably, intralesional injection of nitric oxide donor combined with the embedded WJ-MSCs caused superior recovery of renal I/R injury compared to free WJ-MSCs alone in terms of histopathological scores and renal function indices. Compared to the I/R control group, oxidative stress and inducible nitric oxide synthase (iNOS) expression biomarkers showed a significant decline, whereas endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor (VEGF) expression exhibited a significant increment, indicating regeneration of the injured endothelial tissue. Conclusion The findings confirmed that the hydrogels containing WJ-MSCs and nitric oxide donors can promote the regeneration of renal I/R injuries by increasing angiogenic factors and cell engraftment.

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Rosa, Elisabetta, Carlo Diaferia, Eliana Gianolio, Teresa Sibillano, Enrico Gallo, Giovanni Smaldone, Mariano Stornaiuolo, Cinzia Giannini, Giancarlo Morelli et Antonella Accardo. « Multicomponent Hydrogel Matrices of Fmoc‐FF and Cationic Peptides for Application in Tissue Engineering ». Macromolecular Bioscience, 7 mai 2022, 2200128. http://dx.doi.org/10.1002/mabi.202200128.

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Rosa, Elisabetta, Paolo Pellegrino, Mariafrancesca Cascione, Rosaria Rinaldi, Eliana Gianolio, Charlotte Edwards-Gayle, Ian W. Hamley, Giancarlo Morelli, Antonella Accardo et Carlo Diaferia. « Cross-Link of Telechelic Diacrylate Polyethylene-Glycol in Peptide-Based Fmoc-FF Hydrogel Matrices ». ACS Applied Polymer Materials, 6 juin 2024. http://dx.doi.org/10.1021/acsapm.4c01059.

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Netti, Francesca, Moran Aviv, Yoav Dan, Safra Rudnick-Glick, Michal Halperin-Sternfeld et Lihi Adler-Abramovich. « Stabilizing gelatin-based bioinks under physiological conditions by incorporation of ethylene-glycol-conjugated Fmoc-FF peptides ». Nanoscale, 2022. http://dx.doi.org/10.1039/d1nr08206j.

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Over the last decade, three-dimensional (3D) printing technologies have attracted the interest of researchers due to the possibility of fabricating tissue- and organ-like structures with similarities to the organ of...

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Gallo, Enrico, Carlo Diaferia, Giovanni Smaldone, Elisabetta Rosa, Giovanni Pecoraro, Giancarlo Morelli et Antonella Accardo. « Fmoc-FF hydrogels and nanogels for improved and selective delivery of dexamethasone in leukemic cells and diagnostic applications ». Scientific Reports 14, n^o 1 (30 avril 2024). http://dx.doi.org/10.1038/s41598-024-60145-z.

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AbstractDexamethasone (DEX) is a synthetic analogue of cortisol commonly used for the treatment of different pathological conditions, comprising cancer, ocular disorders, and COVID-19 infection. Its clinical use is hampered by the low solubility and severe side effects due to its systemic administration. The capability of peptide-based nanosystems, like hydrogels (HGs) and nanogels (NGs), to serve as vehicles for the passive targeting of active pharmaceutical ingredients and the selective internalization into leukemic cells has here been demonstrated. Peptide based HGs loaded with DEX were formulated via the “solvent-switch” method, using Fmoc-FF homopeptide as building block. Due to the tight interaction of the drug with the peptidic matrix, a significant stiffening of the gel (G′ = 67.9 kPa) was observed. The corresponding injectable NGs, obtained from the sub-micronization of the HG, in the presence of two stabilizing agents (SPAN®60 and TWEEN®60, 48/52 w/w), were found to be stable up to 90 days, with a mean diameter of 105 nm. NGs do not exhibit hemolytic effects on human serum, moreover they are selectively internalized by RS4;11 leukemic cells over healthy PBMCs, paving the way for the generation of new diagnostic strategies targeting onco-hematological diseases.

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