Bibliographies thématiques / Fluorescence Microscopy, Image Correlation Spectroscopy

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Littérature scientifique sur le sujet « Fluorescence Microscopy, Image Correlation Spectroscopy »

Auteur : Grafiati

Publié le 9 mars 2023

Mis à jour le 31 juillet 2025

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Articles de revues sur le sujet "Fluorescence Microscopy, Image Correlation Spectroscopy"

Clayton, Andrew H. A. "Phase-Sensitive Fluorescence Image Correlation Spectroscopy." International Journal of Molecular Sciences 25, no. 20 (2024): 11165. http://dx.doi.org/10.3390/ijms252011165.

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Fluorescence lifetime imaging microscopy is sensitive to molecular interactions and environments. In homo-dyne frequency-domain fluorescence lifetime imaging microscopy, images of fluorescence objects are acquired at different phase settings of the detector. The detected intensity as a function of detector phase is a sinusoidal function that is sensitive to the lifetime of the fluorescent species. In this paper, the theory of phase-sensitive fluorescence image correlation spectroscopy is described. In this version of lifetime imaging, image correlation spectroscopy analysis (i.e., spatial auto

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Wiseman, Paul. "Introduction to Fluorescence and Image Correlation Spectroscopy." Microscopy and Microanalysis 10, S02 (2004): 246–47. http://dx.doi.org/10.1017/s1431927604886483.

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Bates, Ian R., Paul W. Wiseman, and John W. Hanrahan. "Investigating membrane protein dynamics in living cellsThis paper is one of a selection of papers published in this Special Issue, entitled CSBMCB — Membrane Proteins in Health and Disease." Biochemistry and Cell Biology 84, no. 6 (2006): 825–31. http://dx.doi.org/10.1139/o06-189.

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Live cell imaging is a powerful tool for understanding the function and regulation of membrane proteins. In this review, we briefly discuss 4 fluorescence-microscopy-based techniques for studying the transport dynamics of membrane proteins: fluorescence-correlation spectroscopy, image-correlation spectroscopy, fluorescence recovery after photobleaching, and single-particle and (or) molecule tracking. The advantages and limitations of each approach are illustrated using recent studies of an ion channel and cell adhesion molecules.

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Wiseman, P. W., J. C. Bouwer, S. Peltier, and M. H. Ellisman. "High Speed Two Photon Excitation Microscopy in Live Cell Imaging using Image Correlation Spectroscopy (ICS)." Microscopy and Microanalysis 7, S2 (2001): 22–23. http://dx.doi.org/10.1017/s1431927600026180.

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For live-cell imaging, two-photon excitation microscopy (TPEM) is proving to be a significant technological advancement. The unique features offered by TPEM are the ability to image thick sections, excellent optical sectioning capabilities, low damage to living cells, and less out of focus fluorescence and out of focus photobleaching. of these features, the most useful for the biological microscopist, is optical sectioning. Optical sectioning is an intrinsic property of the two-photon process, whereby, two infrared (IR) photons are absorbed quickly to excite a single UV/blue transition. The pr

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Laňková, Martina, Jana Humpolíčková, Stanislav Vosolsobě, et al. "Determination of Dynamics of Plant Plasma Membrane Proteins with Fluorescence Recovery and Raster Image Correlation Spectroscopy." Microscopy and Microanalysis 22, no. 2 (2016): 290–99. http://dx.doi.org/10.1017/s1431927616000568.

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AbstractA number of fluorescence microscopy techniques are described to study dynamics of fluorescently labeled proteins, lipids, nucleic acids, and whole organelles. However, for studies of plant plasma membrane (PM) proteins, the number of these techniques is still limited because of the high complexity of processes that determine the dynamics of PM proteins and the existence of cell wall. Here, we report on the usage of raster image correlation spectroscopy (RICS) for studies of integral PM proteins in suspension-cultured tobacco cells and show its potential in comparison with the more wide

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Diaspro, Alberto, Giuseppe Chirico, and Maddalena Collini. "Two-photon fluorescence excitation and related techniques in biological microscopy." Quarterly Reviews of Biophysics 38, no. 2 (2005): 97–166. http://dx.doi.org/10.1017/s0033583505004129.

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1. Introduction 982. Historical background of two-photon effects 992.1 2PE 1002.2 Harmonic generation 1002.3 Fluorescence correlation spectroscopy 1003. Basic principles of two-photon excitation of fluorescent molecules and implications for microscopy and spectroscopy 1013.1 General considerations 1013.2 Fluorescence intensity under the 2PE condition 1033.3 Optical consequences of 2PE 1043.4 Saturation effects in 2PE 1083.5 Fluorescence correlation spectroscopy 1093.5.1 Autocorrelation analysis 1103.5.2 Photon-counting histogram analysis 1124. Two-photon-excited probes 1155. Design considerati

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Friaa, Ouided, and Cécile Fradin. "Coincidence Measurements in Dual-Color Confocal Microscopy: A Combined Single-Particle and Fluorescence Correlation Approach." Biophysical Reviews and Letters 09, no. 03 (2014): 249–71. http://dx.doi.org/10.1142/s1793048014400074.

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In this paper we discuss how the coincident detection of mobile particles in dual-color confocal images can be improved. Optimal coincidence detection requires a careful choice of experimental conditions and image acquisition parameters in order to maximize the overlap between the two detection volumes. By measuring this overlap with fluorescence cross-correlation spectroscopy, we show in particular that a small confocal field of view is necessary in order to maintain good coincidence. Most importantly, coincidence detection also requires a dedicated image analysis strategy. Traditionally, two

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Pelicci, Simone, Laura Furia, Mirco Scanarini, Pier Giuseppe Pelicci, Luca Lanzanò, and Mario Faretta. "Novel Tools to Measure Single Molecules Colocalization in Fluorescence Nanoscopy by Image Cross Correlation Spectroscopy." Nanomaterials 12, no. 4 (2022): 686. http://dx.doi.org/10.3390/nano12040686.

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Super Resolution Microscopy revolutionized the approach to the study of molecular interactions by providing new quantitative tools to describe the scale below 100 nanometers. Single Molecule Localization Microscopy (SMLM) reaches a spatial resolution less than 50 nm with a precision in calculating molecule coordinates between 10 and 20 nanometers. However new procedures are required to analyze data from the list of molecular coordinates created by SMLM. We propose new tools based on Image Cross Correlation Spectroscopy (ICCS) to quantify the colocalization of fluorescent signals at single mole

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Pandzic, E., and R. M. Whan. "A Practical Guide to Fluorescence Temporal and Spatial Correlation Spectroscopy." Biophysicist 2, no. 1 (2021): 40–69. http://dx.doi.org/10.35459/tbp.2019.000143.

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ABSTRACT The aim of this article is to introduce the basic principles behind the widely used microscopy tool: fluorescence fluctuation correlation spectroscopy (FFCS). We present the fundamentals behind single spot acquisition (FCS) and its extension to spatiotemporal sampling, which is implemented through image correlation spectroscopy (ICS). The article is an educational guide that introduces theoretic concepts of FCS and some of the ICS techniques, followed by interactive exercises in MATLAB. There, the learner can simulate data time series and the application of various FFCS techniques, as

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Dal Fovo, Alice, Margherita Morello, Anna Mazzinghi, Caterina Toso, Monica Galeotti, and Raffaella Fontana. "Spectral Mapping Techniques for the Stratigraphic and Compositional Characterisation of a 16th-Century Painting." Heritage 7, no. 3 (2024): 1320–33. http://dx.doi.org/10.3390/heritage7030063.

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Identifying a painting’s pigment palette is crucial for comprehending the author’s technique, as well as for evaluating the degradation of the materials. This paper investigates the stratigraphy and pigments distribution of a 16th-century painting from the Uffizi Galleries collection. Firstly, we obtained compositional information through the cross-sectional analysis of samples using scanning electron microscopy. Secondly, we performed elemental mapping using macro-X-ray fluorescence followed by reflectance imaging spectroscopy. The painting image cube was analysed using the spectral correlati

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Thèses sur le sujet "Fluorescence Microscopy, Image Correlation Spectroscopy"

Nicovich, Philip R. "Widefield fluorescence correlation spectroscopy." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/33849.

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Fluorescence correlation spectroscopy has become a standard technique for modern biophysics and single molecule spectroscopy research. Here is presented a novel widefield extension of the established single-point technique. Flow in microfluidic devices was used as a model system for microscopic motion and through widefield fluorescence correlation spectroscopy flow profiles were mapped in three dimensions. The technique presented is shown to be more tolerant to low signal strength, allowing image data with signal-to-noise values as low as 1.4 to produce accurate flow maps as well as utilizi

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BOUZIN, MARGAUX. "Correlazione di Immagini per lo Studio di Processi Dinamici in Sistemi Biologici." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2015. http://hdl.handle.net/10281/94231.

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Dynamic processes are ubiquitous in biological systems: the transport of organelles, proteins and cargoes in the micron-sized heterogeneous cellular environment mainly occurs by Brownian diffusion, while directional flow or drift phenomena contribute to enhance the diffusion-mediated intracellular trafficking and are responsible for the delivery of blood, nutrients and signaling molecules on the larger scale of whole tissues and organs. Motivated by the relevance of transport phenomena in several fields ranging from cell biology to immunology, I adopt and extend the approach of Fluorescence Im

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Gallagher, Joseph. "Adaptive optics for fluorescence correlation spectroscopy." Thesis, Université Grenoble Alpes (ComUE), 2017. http://www.theses.fr/2017GREAY054/document.

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Ce projet de recherche conjugue deux aspects complémentaires : le développement d’un montage de microscopie intégrant un système d'Optique Adaptative (OA) et l’étude de masses cancéreuses (Sphéroïdes Multicellulaires) sous pression mécanique.Ces deux axes seront mutuellement bénéfiques puisque l’implémentation de l’OA rendra possible l’imagerie et les mesures physiques au sein des sphéroïdes ; d’un autre côté, l’étude des sphéroïdes permettra de caractériser les aberrations induites par ce type d’échantillons et de mieux comprendre les exigences sur le système d’OA qu’imposent l’observation de

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Doroshenko, Mikheil [Verfasser]. "Diffusion in heterogeneous systems studied by laser scanning confocal microscopy and fluorescence correlation spectroscopy / Mikheil Doroshenko." Mainz : Universitätsbibliothek Mainz, 2014. http://d-nb.info/104870758X/34.

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Xu, Lei. "Development and application of ultra-sensitive fluorescence spectroscopy and microscopy for biomolecular interaction studies." Doctoral thesis, KTH, Experimentell biomolekylär fysik, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-146181.

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This thesis describes the development of sensitive and high-resolution fluorescence spectroscopic and microscopic techniques and their application to probe biomolecules and their interactions in solution, lipid membrane model systems and in cells. Paper I-IV are largely focused on methodological developments. In paper I, a new fluorescence method based on fluorescence correlation spectroscopy (FCS) for detecting single particles was realized, requiring no fluorescent labeling of the particles. The method can yield information both about the diffusion properties of the particles as well as abou

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Wang, Ruixing. "STED-fluorescence correlation spectroscopy for dynamic observations in cell biology : from theoretical to practical approaches." Thesis, Aix-Marseille, 2018. http://www.theses.fr/2018AIXM0163/document.

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Les techniques de super-résolution offrent un nouvel aperçu de la description de l'organisation moléculaire dynamique de la membrane plasmique. Parmi ces techniques, la microscopie par déplétion d'émission stimulée (stimulated emission depletion, STED) dépasse la limite de diffraction optique et atteint une résolution de quelques dizaines de nanomètres. Il est une technique polyvalente qui peut être combinée avec d'autres techniques telles que la spectroscopie par corrélation de fluorescence (fluorescence correlation spectroscopy, FCS), fournissant des résolutions spatiales et temporelles élev

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Persson, Gustav. "Temporal Modulation in Fluorescence Spectroscopy and Imaging for Biological Applications." Doctoral thesis, KTH, Experimentell biomolekylär fysik, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-10243.

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This thesis explores the benefits of intensity modulation for the purpose of extending the range of applications of fluorescence spectroscopy and imaging in cellular and molecular biology and medicine. Long-lived transient states of fluorescent molecules can, because of their long lifetimes, be used to detect subtle changes in the microenvironment of the molecule. A method for determining the kinetic rates for transitions to and from such states by registration of changes in the average fluorescence intensity related to different modulation of the excitation source is introduced. It combines t

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Le, Andy Vinh. "Blood Microflow Characterization Using Micro-Particle Image Velocimetry and 2-Beam Fluorescence Cross-Correlation Spectroscopy." Thesis, Université d'Ottawa / University of Ottawa, 2020. http://hdl.handle.net/10393/41535.

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Blood flow through microcirculation in both simple and complex geometry has been difficult to predict due to the composition and complex behavior of blood at the microscale. Blood is a dense suspension of deformable red blood cells that is comparable in dimensions to the microchannels that it flows through. As a result, rheological properties at the microscale can vastly differ from bulk rheological properties due to non-continuum effects. To further develop our understanding of blood microflow; experimental techniques should be explored. In this work, we explore micro-particle image velocime

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Vaillancourt, Benoit. "Novel biophysical appliations [sic] of STICS." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=111550.

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The object of this thesis is to present two novel applications of Spatiotemporal Image Correlation Spectroscopy (STICS) to biological systems. STICS is a technique which uses the correlations in pixel intensity fluctuations of an image time series, captured under fluorescence microscopy, to measure the speed and direction of a flowing population of fluorescently labeled molecules. The method was first applied to measure the dynamics of transport vesicles inside growing pollen tubes of lily flowers. The measured vector maps allowed to confirm the presence of actin filaments along the periphery

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Kohram, Maryam. "A Combined Microscopy and Spectroscopy Approach to Study Membrane Biophysics." University of Akron / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=akron1436530389.

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Chapitres de livres sur le sujet "Fluorescence Microscopy, Image Correlation Spectroscopy"

Lacoste, Judith, Charles Vining, Dongmei Zuo, Aleksandrs Spurmanis, and Claire M. Brown. "Optimal Conditions for Live Cell Microscopy and Raster Image Correlation Spectroscopy." In Reviews in Fluorescence 2010. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-9828-6_12.

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Zhou, Qiwei, Zhixin Shi, Shilun Zheng, et al. "The Effect of Modified Styrene Butadiene Rubber Latex on the Properties of Emulsified Asphalt and Mixture." In Lecture Notes in Civil Engineering. Springer Nature Singapore, 2024. http://dx.doi.org/10.1007/978-981-97-4355-1_33.

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AbstractIn order to study the effect of styrene butadiene rubber (SBR) latex at various dosages on the properties of emulsified asphalt and its mixtures as well as to reveal SBR’s modification mechanism and action, the conventional test, dynamic shear rheology test, fluorescence microscopy test, infrared spectroscopy test, contact angle test, wet wheel abrasion test and rutting deformation test were carried out to investigate the conventional properties, rheological properties, microphase structure, adhesive properties and the abrasion resistance of its mixtures, the resistance to water damage

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Kohl, Tobias, and Petra Schwille. "Fluorescence Correlation Spectroscopy with Autofluorescent Proteins." In Microscopy Techniques. Springer Berlin Heidelberg, 2005. http://dx.doi.org/10.1007/b102212.

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Brock, Roland, and Thomas M. Jovin. "Fluorescence Correlation Microscopy (FCM): Fluorescence Correlation Spectroscopy (FCS) in Cell Biology." In Springer Series in Chemical Physics. Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-59542-4_7.

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Moreno, David F., and Martí Aldea. "Coincidence Analysis of Molecular Dynamics by Raster Image Correlation Spectroscopy." In Computer Optimized Microscopy. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9686-5_17.

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Mazza, Davide, Timothy J. Stasevich, Tatiana S. Karpova, and James G. McNally. "Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence Correlation Spectroscopy and Temporal Image Correlation Spectroscopy." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-477-3_12.

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Dumas, D., B. Riquelme, H. Castellini, L. Basciano, N. de Isla, and J. F. Stoltz. "Calibration in fluorescence correlation spectroscopy for measurements of stem cell differentiation kinetic." In EMC 2008 14th European Microscopy Congress 1–5 September 2008, Aachen, Germany. Springer Berlin Heidelberg, 2008. http://dx.doi.org/10.1007/978-3-540-85228-5_86.

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Schwille, Petra, Katrin Heinze, Petra Dittrich, and Elke Haustein. "Two-Photon Fluorescence Correlation Spectroscopy." In Biomedical Optical Imaging. Oxford University PressNew York, NY, 2009. http://dx.doi.org/10.1093/oso/9780195150445.003.0008.

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Abstract Single-molecule-based fluorescence correlation spectroscopy (or FCS) is currently considered one of the most powerful complementary methods in the context of modern fluorescence microscopy. Although the information it provides is primarily of dynamic rather than spatial nature, recent FCS applications particularly in the cellular environment have raised tremendous hopes that this technique, in conjunction with confocal or two-photon imaging, can open fully new ways to investigate and understand complex biological processes on a single-molecule scale. The difference between FCS and sta

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"Fluorescence Correlation Spectroscopy." In Nanoscopy and Multidimensional Optical Fluorescence Microscopy. Chapman and Hall/CRC, 2010. http://dx.doi.org/10.1201/9781420078893-12.

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Shi, Xianke, and Thorsten Wohland. "Fluorescence Correlation Spectroscopy." In Nanoscopy and Multidimensional Optical Fluorescence Microscopy. Chapman and Hall/CRC, 2010. http://dx.doi.org/10.1201/9781420078893-c6.

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Actes de conférences sur le sujet "Fluorescence Microscopy, Image Correlation Spectroscopy"

Maiti, Sudipta. "Critical properties of the lipid bilayer probed by fluorescence correlation spectroscopy and its variants." In Multiphoton Microscopy in the Biomedical Sciences XXV, edited by Ammasi Periasamy, Peter T. So, and Karsten König. SPIE, 2025. https://doi.org/10.1117/12.3046129.

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Webb, Watt W. "Multiphoton Microscopy MPM: Imaging Spectra and Dynamics of Molecular Function Deep in Living Tissues." In In Vivo optical Imaging at the NIH. Optica Publishing Group, 1999. http://dx.doi.org/10.1364/ivoi.1999.msi3.

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Multiphoton Excitation (MPE) of fluorescence provides the optimum photophysics for microscopic imaging deep in living tissue with minimal photodamage, to depths so far ~ 400 µm. Tissue autofluorescence excited by two-photon or three-photon absorption to ultra-violet energies can provide specific indications of disease. Useful autofluorescence of serotonin (5HT), melatonin, indolamine breakdown products, NADH, collagen, elastin, and a number of yet-to-be-identified molecular species, some of which identify disease states are already being imaged routinely. For research in model animals, genetic

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Gregor, Ingo, Niels Rademacher, Max Tillmann, Matthias Patting, Jörg Enderlein, and Felix Koberling. "Fluorescence lifetime image scanning microscopy." In Single Molecule Spectroscopy and Superresolution Imaging XV, edited by Ingo Gregor, Rainer Erdmann, and Felix Koberling. SPIE, 2022. http://dx.doi.org/10.1117/12.2625458.

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Liu, Yafeng, Tongsheng Chen, and Qingming Luo. "Fluorescence correlation spectroscopy based upon two-photon excitation." In Biomolecular photonoics and Multidimensional Microscopy, edited by Qingming Luo and Min Gu. SPIE, 2003. http://dx.doi.org/10.1117/12.546244.

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Gregor, Ingo, Niels Radmacher, and Jörg Enderlein. "Fluorescence lifetime image scanning microscopy (Conference Presentation)." In Single Molecule Spectroscopy and Superresolution Imaging XIII, edited by Ingo Gregor, Rainer Erdmann, and Felix Koberling. SPIE, 2020. http://dx.doi.org/10.1117/12.2546532.

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Krmpot, Aleksandar J., Stanko N. Nikolić, Marco Vitali, et al. "Quantitative confocal fluorescence microscopy of dynamic processes by multifocal fluorescence correlation spectroscopy." In European Conference on Biomedical Optics. OSA, 2015. http://dx.doi.org/10.1364/ecbo.2015.95360o.

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Krmpot, Aleksandar J., Stanko N. Nikolić, Marco Vitali, et al. "Quantitative confocal fluorescence microscopy of dynamic processes by multifocal fluorescence correlation spectroscopy." In European Conferences on Biomedical Optics, edited by Emmanuel Beaurepaire, Peter T. C. So, Francesco Pavone, and Elizabeth M. Hillman. SPIE, 2015. http://dx.doi.org/10.1117/12.2183935.

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Sarkar, Anirban, Irène Wang, Aditya Katti, Jörg Enderlein, Jacques Derouard, and Antoine Delon. "Fluorescence speckle image correlation spectroscopy (Conference Presentation)." In Unconventional Optical Imaging II, edited by Corinne Fournier, Marc P. Georges, and Gabriel Popescu. SPIE, 2020. http://dx.doi.org/10.1117/12.2558129.

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Li, Yilun. "Variance lower bound on fluorescence microscopy image denoising." In High-Speed Biomedical Imaging and Spectroscopy VIII, edited by Keisuke Goda and Kevin K. Tsia. SPIE, 2023. http://dx.doi.org/10.1117/12.2647750.

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Sarkar, Soumyajit, Mohammad Zaffar, and Hari M. Varma. "Laser speckle correlation microscopy system to image microvasculature perfusion." In Diffuse Optical Spectroscopy and Imaging, edited by Davide Contini, Yoko Hoshi, and Thomas D. O'Sullivan. SPIE, 2023. http://dx.doi.org/10.1117/12.2670792.

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