Littérature scientifique sur le sujet « Flippases »
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Articles de revues sur le sujet "Flippases"
Takeda, Miyoko, Kanako Yamagami et Kazuma Tanaka. « Role of Phosphatidylserine in Phospholipid Flippase-Mediated Vesicle Transport in Saccharomyces cerevisiae ». Eukaryotic Cell 13, no 3 (3 janvier 2014) : 363–75. http://dx.doi.org/10.1128/ec.00279-13.
Texte intégralJing, Weidong, Mehmet Yabas, Angelika Bröer, Lucy Coupland, Elizabeth E. Gardiner, Anselm Enders et Stefan Bröer. « Calpain cleaves phospholipid flippase ATP8A1 during apoptosis in platelets ». Blood Advances 3, no 3 (23 janvier 2019) : 219–29. http://dx.doi.org/10.1182/bloodadvances.2018023473.
Texte intégralSlavetinsky, Christoph J., Andreas Peschel et Christoph M. Ernst. « Alanyl-Phosphatidylglycerol and Lysyl-Phosphatidylglycerol Are Translocated by the Same MprF Flippases and Have Similar Capacities To Protect against the Antibiotic Daptomycin in Staphylococcus aureus ». Antimicrobial Agents and Chemotherapy 56, no 7 (9 avril 2012) : 3492–97. http://dx.doi.org/10.1128/aac.00370-12.
Texte intégralMENON, A. « Flippases ». Trends in Cell Biology 5, no 9 (septembre 1995) : 355–60. http://dx.doi.org/10.1016/s0962-8924(00)89069-8.
Texte intégralWatkins, W. E., III III et A. K. Menon. « Reconstitution of Phospholipid Flippase Activity from E. coli Inner Membrane : A Test of the Protein Translocon as a Candidate Flippase ». Biological Chemistry 383, no 9 (17 septembre 2002) : 1435–40. http://dx.doi.org/10.1515/bc.2002.162.
Texte intégralDevaux, Philippe F. « Phospholipid flippases ». FEBS Letters 234, no 1 (4 juillet 1988) : 8–12. http://dx.doi.org/10.1016/0014-5793(88)81291-2.
Texte intégralDaleke, David L. « Phospholipid Flippases ». Journal of Biological Chemistry 282, no 2 (27 novembre 2006) : 821–25. http://dx.doi.org/10.1074/jbc.r600035200.
Texte intégralBasante-Bedoya, Miguel A., Stéphanie Bogliolo, Rocio Garcia-Rodas, Oscar Zaragoza, Robert A. Arkowitz et Martine Bassilana. « Two distinct lipid transporters together regulate invasive filamentous growth in the human fungal pathogen Candida albicans ». PLOS Genetics 18, no 12 (14 décembre 2022) : e1010549. http://dx.doi.org/10.1371/journal.pgen.1010549.
Texte intégralRajasekharan, Archita, Vincent Gerard Francis et Sathyanarayana N. Gummadi. « Biochemical evidence for energy-independent flippase activity in bovine epididymal sperm membranes : an insight into membrane biogenesis ». REPRODUCTION 146, no 3 (septembre 2013) : 209–20. http://dx.doi.org/10.1530/rep-13-0121.
Texte intégralLenoir, Guillaume, et Joost C. M. Holthuis. « The elusive flippases ». Current Biology 14, no 21 (novembre 2004) : R912—R913. http://dx.doi.org/10.1016/j.cub.2004.10.008.
Texte intégralThèses sur le sujet "Flippases"
McDowell, Stephen C. « Lipid Flippases and Elemental Homeostasis Systems in Arabidopsis thaliana ». Thesis, University of Nevada, Reno, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=3566276.
Texte intégralMany molecules in living systems are present in charged forms, and these molecules are often highly regulated. The work presented in the following chapters addresses two main topics involving charged molecules using the model plant Arabidopsis thaliana: elemental homeostasis and lipid flippases. The study of elemental homeostasis is referred to as ionomics and is the topic of Chapter II. P4-ATPases are thought to be the principle class of proteins with lipid flippase activity and are the topics of Chapter III and Chapter IV.
Plants, especially seed crops, are an important source of mineral nutrition in the human diet and are thus important targets for biofortification and toxic element exclusion. Here, we report the results of a pilot ionomic screen in which we quantified the concentrations of 14 elements in Arabidopsis seeds. To identify conditional ionomic phenotypes, plants were grown under four different soil conditions: standard, or modified with NaCl, heavy metals, or alkali. To help identify the genetic networks regulating the seed ionome, elemental concentrations were evaluated in mutants corresponding to 760 genes as well as 10 naturally occurring accessions. The frequency of ionomic phenotypes observed in the mutant screen supports an estimate that up to 11% of the Arabidopsis genome encodes proteins of functional relevance to the seed ionome. A subset of mutants were analyzed with two independent alleles, providing five examples of genes important for regulation of the seed ionome: SOS2, ABH1, CCC, At3g14280, and CNGC2. Reproducible ionomic differences were also observed between the Col-0 reference accession and eight of the other nine accessions screened. Significantly, all 15 mutants with reproducible ionomic phenotypes showed at least one change under standard soil conditions. This suggests that the sole use of a standard growth environment might be the most effective strategy for continued reverse-genetic efforts to identify genes that impact the Arabidopsis seed ionome. Nonetheless, each soil modification had a unique impact on the Col-0 seed ionome and elicited several conditional phenotypes in both the mutant and accession screens, indicating that seed elemental homeostasis is sensitive to soil conditions. Together, the results of this study establish that elemental analysis is a sensitive approach to identify genes and environmental conditions that impact elemental accumulation in Arabidopsis seed.
By flipping lipids between membrane leaflets, P4-ATPases are thought to help create and maintain asymmetry in biological membranes. Lipid asymmetry between membrane leaflets has been implicated in a wide range of biological processes including: vesicular trafficking, cell signaling, modulation of membrane permeability, protein recruitment, and regulation of protein activity. Additionally, one P4-ATPase, Neo1p, is essential in yeast. In Arabidopsis thaliana, 12 P4-ATPases have been identified: Aminophospholipid ATPase 1 (ALA1) to ALA12. However, very little is known about P4-ATPases in the context of plant systems.
Of the 12 ALA isoforms, only ALA3 has been extensively studied. Previous studies have shown that loss of ALA3 results in pleiotropic phenotypes affecting root, shoot, and reproductive development. Here, we expand on the previous studies by showing that multiple phenotypes for ala3 mutants are strongly sensitive to growth conditions. We also expand on the ala3 pollen phenotype by identifying three points of defect in ala3 pollen tubes: delayed germination, slow growth, and reduced overall length. Furthermore, we show that ala3 pistils have reduced ovule production, thus providing the first evidence of a female reproductive defect in ala3 mutants. Together, these results support a model in which ALA3 functions in multiple cell types and is critical to plants for development and adaptation to varied growth conditions.
Two other ALA isoforms, ALA6 and ALA7, were also examined in this study. We provide in-vitro and in-vivo evidence that ALA6 and ALA7 are important for rapid, sustained pollen tube growth. Expression of fluorescently labeled ALA6 fusion proteins indicates that the subcellular localization of ALA6 includes the plasma membrane and highly mobile endomembrane structures. We also show that staining by lipophilic FM dyes is reduced by ∼10-fold in ala6-1/7-2 pollen tubes relative to wild-type, suggesting differences in plasma membrane composition. Furthermore, tandem mass spectroscopy analysis revealed significant differences between the lipid compositions of ala6-1/7-2 and wild-type pollen grains, both in the concentrations of different headgroups and in the average number of double bonds present within acyl side chains. Together, these results support a model in which ALA6 and ALA7 function to directly or indirectly regulate the distribution and concentration of lipids in pollen and are thus critical for pollen fitness.
Lamy, Anaïs. « Lipid Flippases from Plasmodium Parasites : from Heterologous Production towards Functional Characterization ». Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS447/document.
Texte intégralMalaria is a devastating disease caused by a parasite of the genus Plasmodium. Due to the spread of strains resistant to current antimalarial drugs, it is necessary to understand essential physiological functions of the parasite in order to find new drug targets. Membrane transport proteins are an important class of drug targets in humans, as they perform essential physiological roles of the cell. However, for Plasmodium parasites, just a few membrane transporters have been biochemically described. Recent gene-deletion studies in malaria mouse models have shown that the Plasmodium P4-ATPase, or lipid flippase, ATP2 is essential for the parasite. In eukaryotes, the phospholipid translocation activity of P4-ATPases is needed to maintain the asymmetric distribution of membranes, a key element in many essential processes like vesicle budding or apoptosis. Lipid flippases form heteromeric complexes with members of the Cdc50 protein family, also found in the genomes of Plasmodium parasites. To understand the functional role of these still putative transporters during malaria infection we need to study their transport mechanism and identify their substrate(s). We have conducted the heterologous expression in Saccharomyces cerevisiae of ATP2 in complex with the Cdc50 subunits from three different Plasmodium species. We succeeded to co-express the ATP2 ortholog of P. chabaudi (PcATP2) and the related putative PcCdc50 proteins. By co-immunoprecipitation and Fluorescence-detection Size Exclusion Chromatography, we have managed to identify the Cdc50 β-subunit that associates to PcATP2: PcCdc50.1. We then purified the complex PcATP2/PcCdc50.1 using immobilized nanobodies that recognize the GFP fused at the C-terminal end of PcATP2 and we initiated the functional characterization using ATPase and phosphorylation activity assays
Basante-Bedoya, Miguel Angel. « Transporteurs lipidiques dans la morphogenèse du champignon pathogène opportuniste de l’Homme Candida albicans ». Electronic Thesis or Diss., Université Côte d'Azur, 2021. http://theses.univ-cotedazur.fr/2021COAZ6030.
Texte intégralCandida albicans is a human opportunistic fungal pathogen that can cause superficial or systemic infections; its ability to change from an ovoid to a filamentous form is associated with its virulence. During this highly polarized filamentous growth, an accumulation of vesicles (Spitzenkörper), characteristic of filamentous fungi, as well as a polarized distribution of lipids, such as ergosterol, phosphorylated derivatives of phosphatidylinositol (PI(4)P, PI(4,5)P2) and phosphatidylserine (PS) is observed at the apex of filaments. However, the importance of the asymmetry of these lipids in the membrane bilayer is not completely understood. Flippases (P4-ATPases) transport lipids across the membrane bilayer to generate and maintain its asymmetry. C. albicans has 5 flippases, including Drs2 which is critical for filamentous growth and phosphatidylserine (PS) distribution. Furthermore, a drs2 deletion mutant is hypersensitive to fluconazole and copper. We show here that such a mutant is also critical to virulence in a mouse model of systemic infection. To clarify the role of Drs2 during C. albicans filamentous growth, we studied the distribution of this ATPase, as well as that of key lipids and regulators, during the initiation and maintenance of this growth process. We also characterized point mutants of Drs2, analogous to those altered for PS transport in S. cerevisiae. In addition, we examined the importance of other flippases, such as Dnf1-3, in invasive growth and the role of lipid transporters belonging to the oxysterol binding protein (Osh) family. Our results indicate in particular that Drs2 plays a unique role in the maintenance of invasive filamentous growth of C. albicans, which appears to be more critical after the first septum formation, and that an interaction between Drs2 and Osh4, via PI(4)P, plays an essential role during invasive filamentous growth
Ezanno, Pierre. « Flippase, tension mécanique et mécanosensibilité ». Paris 6, 2009. http://www.theses.fr/2009PA066167.
Texte intégralMembrane proteins and soluble proteins are sensitive to their environment. The lateral mechanical tension in membrane via lipids is a physico-chemical parameter of membrane protein environment. The flippase (a membrane protein) can modulate this tension creating an asymmetry of lipids populations between both of membrane leaflets. Flippase from erythrocytes (a Mg ATP dependent ATPase) is, in this thesis, used unpurified: from its native membrane. A mechanosensitive membrane protein changes conformation according to the mechanical tension in the membrane; for example, the MscL (Mechano sensitive channel Large conductance). The flippase is still active in giant membrane systems used, despite a partial dehydration step of membrane (required step in making giant liposomes). An addition of MG ATP triggers the flippase activity which is detected by liposome shape changes. Then, the lateral mechanical tension is triggered in a membrane containing the flippase and the MscL the opening of which is monitored by electrophysiology. In the presence of flippase activity, the MscL’s behaviour is modified: the required tension to open the channel seems to be lowered
Dieudonne, Thibaud. « Functional and Structural Characterization of Lipid Flippases : The Yeast Drs2p/Cdc50p and the Disease-Related Human Atp8b1/Cdc50a Complexes Structure and Autoregulation of a P4-ATPase Lipid Flippase Screening of Detergents for Stabilization of Functional Membrane Proteins High phosphatidylinositol 4-phosphate (PI4P)-dependent ATPase activity for the Drs2p-Cdc50p flippase after removal of its N- and C-terminal extensions Slow Phospholipid Exchange between a Detergent-Solubilized Membrane Protein and Lipid-Detergent Mixed Micelles : Brominated Phospholipids as Tools to Follow Its Kinetics ». Thesis, université Paris-Saclay, 2020. http://www.theses.fr/2020UPASS023.
Texte intégralLiving cells are surrounded by membranes organized in bilayers, separating the intracellular medium from the extracellular environment. A hallmark of eukaryotic membranes from the late secretory/endocytic pathways is the asymmetric distribution of phospholipids between the two leaflets. Indeed, phosphatidylcholine (PC) and sphingolipids (SL) are mainly found in the outer leaflet whereas phosphatidylserine (PS) and phosphatidylethanolamine (PE) are sequestered in the inner leaflet. This asymmetry is maintained thanks to different membrane lipid transporters. Among them, flippases, which are transporters fueled by ATP hydrolysis, translocate lipids from the outer to the inner leaflet. Flippases belong to the P4-ATPase family and have been linked to several diseases. For instance, mutated forms of a human P4-ATPase, ATP8B1, are responsible for intrahepatic cholestasis, a severe liver disease. In this thesis, we investigated the regulatory mechanism of two flippases, the yeast PS-specific flippase complex Drs2p/Cdc50p, and the human disease-related flippase complex ATP8B1/CDC50A. Both proteins were expressed in S. cerevisiae and purified for downstream functional characterization. Our results demonstrate that both flippases are tightly regulated by phosphoinositides and autoinhibited by their N- and C-terminal extensions
Johansson, Martin. « Instruktionsfilmer och det flippade klassrummet - Det flippade klassrummet, varför inte ? » Thesis, Malmö universitet, Fakulteten för lärande och samhälle (LS), 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-34640.
Texte intégralAndersson, Hanna. « Den flippade läxan : En systematisk litteraturstudie av läxor i det flippade matematikklassrummet ». Thesis, Linköpings universitet, Matematik och tillämpad matematik, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-153116.
Texte intégralThe purpose of this study is to investigate homework given in the flipped mathematics classroom. One of the characteristics of the “flipped classroom” is that traditional lectures are not placed in class time. Direct instruction is instead given as homework, “flipped homework”, often in the form of video lectures. The literature review is based on nine articles and focuses on the design of flipped mathematics homework, pupil’s views of the method, and the possible advantages and disadvantages of flipped homework in relation to traditional homework. There is still a lack of research done on “flipped homework”, which makes it difficult to draw any general conclusions. However, the results indicate that the teaching method may have some advantages, including that video lectures gives the students a greater responsibility for their own learning, and that the fixed time of the video have the potential to reduce the difference in time spent by different students on the same homework.
Villazana-Kretzer, Diana L. « Giardia lamblia genomic and molecular analyses of flippase / ». To access this resource online via ProQuest Dissertations and Theses @ UTEP, 2008. http://0-proquest.umi.com.lib.utep.edu/login?COPT=REJTPTU0YmImSU5UPTAmVkVSPTI=&clientId=2515.
Texte intégralPeters, Ida. « Det flippade klassrummet : ur ett elevperspektiv ». Thesis, Högskolan Kristianstad, Sektionen för lärande och miljö, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:hkr:diva-13239.
Texte intégralNaito, Tomoki. « Phospholipid Flippase Activity and Cellular Function of Class 5 P4-ATPases ». 京都大学 (Kyoto University), 2017. http://hdl.handle.net/2433/225530.
Texte intégralLivres sur le sujet "Flippases"
Edizioni, Poiana, et Matteo Maschio. Nft : Monetizzare con I Token Non Fungibili - Come Utilizzare le Criptovalute Attraverso le Blockchain per Creare, Acquistare, Vendere e Flippare NFT. Independently Published, 2022.
Trouver le texte intégralHoward, James. NFT : Monetizzare con l'Arte Digitale e le Blockchain : I Migliori Casi Studio per Imparare a Creare, Vendere, Acquistare e Flippare Non-Fungible Tokens. Independently Published, 2022.
Trouver le texte intégralChapitres de livres sur le sujet "Flippases"
Roelofsen, Ben, Esther Middelkoop, Willem P. Vermeulen, Alexander J. Smith et J. A. F. Op den Kamp. « Phospholipid Flippases : Neither Exclusively, Nor Only Involved In Maintaining Membrane Phospholipid Asymmetry ». Dans Molecular Dynamics of Biomembranes, 367–82. Berlin, Heidelberg : Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-61126-1_29.
Texte intégralPollock, Naomi L., Petra H. M. Niesten et Richard Callaghan. « The Flippase Delusion ? » Dans Transmembrane Dynamics of Lipids, 225–49. Hoboken, NJ, USA : John Wiley & Sons, Inc., 2011. http://dx.doi.org/10.1002/9781118120118.ch11.
Texte intégralCook, Shelley M., et David L. Daleke. « Substrate Specificity of the Aminophospholipid Flippase ». Dans Transmembrane Dynamics of Lipids, 199–223. Hoboken, NJ, USA : John Wiley & Sons, Inc., 2011. http://dx.doi.org/10.1002/9781118120118.ch10.
Texte intégralThélot, François A., et Maofu Liao. « Cryo-EM Analysis of the Lipopolysaccharide Flippase MsbA ». Dans Lipopolysaccharide Transport, 233–47. New York, NY : Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2581-1_14.
Texte intégralJensen, Maria S., Sara Costa, Thomas Günther-Pomorski et Rosa L. López-Marqués. « Cell-Based Lipid Flippase Assay Employing Fluorescent Lipid Derivatives ». Dans P-Type ATPases, 371–82. New York, NY : Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3179-8_33.
Texte intégralPaulusma, C. C., A. Groen, C. Kunne, K. S. Ho-Mok, D. E. Folmer, D. R. De Waart, L. N. Bull et R. P. J. Oude Elferink. « ATP8B1, a phosphatidylserine flippase deficient in inherited intrahepatic cholestasis ». Dans Bile Acid Biology and Therapeutic Actions, 9–17. Dordrecht : Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-1-4020-9644-0_2.
Texte intégralMarek, Magdalena, et Thomas Günther-Pomorski. « Assay of Flippase Activity in Proteoliposomes Using Fluorescent Lipid Derivatives ». Dans P-Type ATPases, 181–91. New York, NY : Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3179-8_18.
Texte intégralDieudonné, Thibaud, Christine Jaxel, Maylis Lejeune, Guillaume Lenoir et Cédric Montigny. « Expression in Saccharomyces cerevisiae and Purification of a Human Phospholipid Flippase ». Dans Methods in Molecular Biology, 231–46. New York, NY : Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3147-8_13.
Texte intégralAzouaoui, Hassina, Cédric Montigny, Aurore Jacquot, Raphaëlle Barry, Philippe Champeil et Guillaume Lenoir. « Coordinated Overexpression in Yeast of a P4-ATPase and Its Associated Cdc50 Subunit : The Case of the Drs2p/Cdc50p Lipid Flippase Complex ». Dans P-Type ATPases, 37–55. New York, NY : Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3179-8_6.
Texte intégralMontigny, Cédric, Hassina Azouaoui, Aurore Jacquot, Marc le Maire, Christine Jaxel, Philippe Champeil et Guillaume Lenoir. « Overexpression of Membrane Proteins in Saccharomyces cerevisiae for Structural and Functional Studies : A Focus on the Rabbit Ca2+-ATPase Serca1a and on the Yeast Lipid “Flippase” Complex Drs2p/Cdc50p ». Dans Membrane Proteins Production for Structural Analysis, 133–71. New York, NY : Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0662-8_6.
Texte intégralActes de conférences sur le sujet "Flippases"
Calianu, Andreea, et Radu Tamaian. « Computational Design of New Teixobactin Analogues as Inhibitors of Lipid II Flippase MurJ ». Dans ECMC 2022. Basel Switzerland : MDPI, 2022. http://dx.doi.org/10.3390/ecmc2022-13295.
Texte intégralRapports d'organisations sur le sujet "Flippases"
Harper, Jeffrey F. Final Report for DE-FG02-04ER15626 : P-type ATPases in Plants – Role of Lipid Flippases in Membrane Biogenesis. Office of Scientific and Technical Information (OSTI), février 2015. http://dx.doi.org/10.2172/1223536.
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