Littérature scientifique sur le sujet « FLIMM method »
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Articles de revues sur le sujet "FLIMM method"
Zhang, Beini, Liping Li, Yetao Lyu, Shuguang Chen, Lin Xu et Guanhua Chen. « A New Instrument Monitoring Method Based on Few-Shot Learning ». Applied Sciences 13, no 8 (21 avril 2023) : 5185. http://dx.doi.org/10.3390/app13085185.
Texte intégralWang, Quan, Yahui Li, Dong Xiao, Zhenya Zang, Zi’ao Jiao, Yu Chen et David Day Uei Li. « Simple and Robust Deep Learning Approach for Fast Fluorescence Lifetime Imaging ». Sensors 22, no 19 (26 septembre 2022) : 7293. http://dx.doi.org/10.3390/s22197293.
Texte intégralNeedham, Sarah R., Laura C. Zanetti-Domingues, Kathrin M. Scherer, Michael Hirsch, Daniel J. Rolfe, Selene K. Roberts, Marisa L. Martin-Fernandez, David T. Clarke et Christopher J. Tynan. « Determining the geometry of oligomers of the human epidermal growth factor family on cells with <10 nm resolution ». Biochemical Society Transactions 43, no 3 (1 juin 2015) : 309–14. http://dx.doi.org/10.1042/bst20140318.
Texte intégralJi, Mingmei, Jiahui Zhong, Runzhe Xue, Wenhua Su, Yawei Kong, Yiyan Fei, Jiong Ma, Yulan Wang et Lan Mi. « Early Detection of Cervical Cancer by Fluorescence Lifetime Imaging Microscopy Combined with Unsupervised Machine Learning ». International Journal of Molecular Sciences 23, no 19 (29 septembre 2022) : 11476. http://dx.doi.org/10.3390/ijms231911476.
Texte intégralPande, P., C. A. Trivedi et J. A. Jo. « Analysis of Fluorescence Lifetime Imaging Microscopy (FLIM) Data ». Methods of Information in Medicine 49, no 05 (2010) : 531–36. http://dx.doi.org/10.3414/me09-02-0046.
Texte intégralLiu, Guoying, Pengwei Li et Yun Zhang. « A Color Texture Image Segmentation Method Based on Fuzzy c-Means Clustering and Region-Level Markov Random Field Model ». Mathematical Problems in Engineering 2015 (2015) : 1–9. http://dx.doi.org/10.1155/2015/240354.
Texte intégralXU, LINGLING, ZHONG-CHAO WEI, SHAOQUN ZENG et ZHEN-LI HUANG. « QUANTIFYING THE SHORT LIFETIME WITH TCSPC-FLIM : FIRST MOMENT VERSUS FITTING METHODS ». Journal of Innovative Optical Health Sciences 06, no 04 (octobre 2013) : 1350030. http://dx.doi.org/10.1142/s1793545813500302.
Texte intégralJi, Chao, Xing Wang, Kai He, Yanhua Xue, Yahui Li, Liwei Xin, Wei Zhao, Jinshou Tian et Liang Sheng. « Compressed fluorescence lifetime imaging via combined TV-based and deep priors ». PLOS ONE 17, no 8 (12 août 2022) : e0271441. http://dx.doi.org/10.1371/journal.pone.0271441.
Texte intégralMikicin, Mirosław. « Relationships of attention and arousal are responsible for action in sports ». Biomedical Human Kinetics 14, no 1 (1 janvier 2022) : 229–35. http://dx.doi.org/10.2478/bhk-2022-0028.
Texte intégralAdhikari, Mou, Rola Houhou, Julian Hniopek et Thomas Bocklitz. « Review of Fluorescence Lifetime Imaging Microscopy (FLIM) Data Analysis Using Machine Learning ». Journal of Experimental and Theoretical Analyses 1, no 1 (21 septembre 2023) : 44–63. http://dx.doi.org/10.3390/jeta1010004.
Texte intégralThèses sur le sujet "FLIMM method"
Mendoza, Lopez Duvan Alexander. « Étude des phénomènes de piégeage et dépiégeage de charges par mesures de charge d'espace et de décharge photo-stimulée dans des films polymères minces pour le stockage d'énergie ». Electronic Thesis or Diss., Toulouse 3, 2023. http://www.theses.fr/2023TOU30364.
Texte intégralWith the development of the polymer film capacitor market, there is growing interest in the study of charge generation, transport and trapping phenomena within relatively thin dielectric films. Indeed, increased requirements imposed by high supply voltages or elevated temperature conditions favor the appearance of charges which, as they migrate through the material, can be trapped to create space charge. This charge trapping dynamic is susceptible to causing localized intensifications of the electric field, thereby engendering electromechanical stresses that may ultimately culminate in material failure. Consequently, it is imperative to investigate the nature and properties of traps found in polymer dielectrics. The aim is to enrich our understanding of these materials and enhance the reliability of the systems in which they are incorporated. Among the available experimental approaches for investigating the electrical behavior of polymers, those capable of providing specific insights into the energy states of traps remain relatively scarce. The most prevalent methods in this regard are probably the Thermally Stimulated Discharge (TSD) and Photo-Stimulated Discharge (PSD) techniques. The PSD method, which entails measuring discharge currents during exposure to light within the UV-visible spectrum, offers the capability to identify interactions between mobile or trapped charges and the energy of incident photons. This approach possesses an advantage over the TSD method, which relies on a temperature ramp, as it does not induce alterations or destruction of traps through thermal effects. However, it is worth noting that interpreting PSD spectra poses challenges, as the measured current may originate from diverse phenomena, including photogeneration of charges or photoinjection of carriers, all of which are likely to interact with the existing traps. In an attempt to elucidate the underlying mechanisms governing in photoinduced current, we propose to couple PSD measurements to space charge measurements. By implementing protocols that systematically manipulate polarization and irradiation parameters, changes in the density or position of charges will serve as valuable indicators of the origin of charges and the kinetics of their trapping and detrapping processes. To achieve this objective, we intend to employ the Light Intensity Modulation Method (LIMM), which is particularly well-suited for the investigation of thin films (with dimensions on the order of a few micrometers) and offers excellent spatial resolution in proximity to interfaces. It will also be an asset for studying electric field reinforcement effects, linked to the use of an interdigitated electrode for PSD measurement. The integration of LIMM and PSD methods within the same experimental setup, and their use in sequential measurement programs, offers the possibility of continuously monitoring trap filling, trap release by light irradiation, and exploring the relationships between applied light energy and trap characteristics. In fact, the measurement system demonstrates in an innovative approach that the reduction in space charge density after PSD measurements is directly attributed to the light disturbance itself, rather than being influenced by concurrent factors such as the time elapsed after the charge period or manipulations leading to charge release
Hosny, Neveen Amera. « Development of a non-invasive method to detect pericellular spatial oxygen gradients using FLIM ». Thesis, Queen Mary, University of London, 2011. http://qmro.qmul.ac.uk/xmlui/handle/123456789/1262.
Texte intégralFERRI, Gianmarco. « A biophysical approach to the study of living β-cell ». Doctoral thesis, Scuola Normale Superiore, 2020. http://hdl.handle.net/11384/91107.
Texte intégralChakraborty, Sandeep, et 夏柏杉. « Fluorescence based methods (FLIM and FRET) to study the metabolic state of Parkinson’s disease in cell model and in vitro protein intractions ». Thesis, 2016. http://ndltd.ncl.edu.tw/handle/41738728403186503249.
Texte intégral國立陽明大學
生醫光電研究所
104
Fluorescence based spectroscopic and microscopic techniques have been widely used in the field of scientific research and medical diagnostics for their unique advantages, such as specificity, sensitivity, simplicity, and speed. Over the years, two of the most frequently used techniques based on fluorescence are Förster/fluorescence resonance energy transfer (FRET) spectroscopy (and microscopy) and fluorescence lifetime imaging microscopy (FLIM). Fluorescence lifetime imaging technique quantifies the average time a fluorophore remains in the excited state before descending to the ground state. This technique can easily distinguish two molecules with similar fluorescence emission bands, or the same molecule with different structural conformations based on their fluorescence lifetime. In this work, we applied two-photon fluorescence lifetime imaging microscopy (2P-FLIM) to observe two endogenous fluorescent molecules viz. reduced nicotinamide adenine dinucleotide (NADH) and oxidized flavin adenine dinucleotide (FAD). NADH have longer lifetime when it binds to protein and free NADH has shorter lifetime. On the other hand, FAD has shorter lifetime when it binds to protein, while the free FAD has longer time. In this thesis, we exploited these properties of NADH and FAD to monitor the cellular metabolic state in Parkinson’s disease (PD) cellular model in terms of the cellular redox ratios NADH/NAD+ and FADH2/FAD via 2P-FLIM. NADH and FAD are two co-enzymes which take part in the ATP production of cells in the inner-mitochondrial membrane; we monitored via 2P-FLIM to map the cellular metabolism in PD. The cellular redox state can be interpreted in terms of the fluorescence lifetime components values of NADH and FAD, and also the relative contributions of free to protein-bound NADH (and FAD). Two-photon excitation was used for lifetime imaging of NADH and FAD, as it causes less photobleaching and yields higher cell viability. PD is a progressive neurological disorder due to the loss of dopaminergic neurons in substantia nigra of mid-brain region and several lines of evidence suggest that mitochondrial dysfunction is responsible for the disease pathology. In this work, PC12 cells were first treated with nerve growth factor (NGF) to differentiate it into neuronal cells which were further treated with 1-methyl-4-phenylpyridinium (MPP+) to establish the PD cellular model. A systematic FLIM data analysis showed a statistically significant (p < 0.001) decrease in the fluorescence lifetime of both free and protein-bound NADH, as well as free and protein-bound FAD in MPP+ treated cells. On the relative contributions of the free and protein-bound NADH and FAD to the life time, however, both the free NADH contribution and the corresponding protein-bound FAD contribution increased significantly (p < 0.001) in MPP+ treated cells, compared to control cells. These results, which indicate a shift in energy production in the MPP+ treated cells from oxidative phosphorylation towards anaerobic glycolysis, can potentially be used as cellular metabolic metrics to assess the condition of PD at the cellular level. In this thesis, we also developed an organic fluorophore based steady-state quantitative FRET assay with a new and modified algorithm to extract FRET emission signal. This method was further applied to quantify the interaction between leukocyte function-associated antigen-1(LFA-1) and intercellular adhesion molecule-1 (ICAM-1) in terms of the dissociation constant (Kd). The interaction between these two transmembrane proteins plays a significant role in cellular adhesion including the extravasation and inflammatory response of leukocytes, and also in the formation of immunological synapse. Moreover, the LFA-1/ICAM-1 interaction may serve as a potential therapeutic target for the treatment of several diseases as irregular expressions of LFA-1 or ICAM-1 or both may lead to autoimmune diseases, metastasis cancer, etc. In addition, we also developed the FRET assay into a screening platform to identify peptides and small molecules that inhibit the LFA-1/ICAM-1 interaction. For the FRET pair, we used Alexa Fluor 488-LFA-1 conjugate as the donor and Alexa Fluor 555-human recombinant ICAM-1 (D1-D2-Fc) as the acceptor. From our quantitative FRET analysis, the Kd between LFA-1 and D1-D2-Fc was determined to be 17.93 ± 1.34 nM. Both the Kd determination and screening assay were performed in a 96-well plate platform, providing the opportunity to develop it into a high-throughput assay. In future, these approaches can be further developed/optimized for the study of in vivo Parkinson’s disease pathogenesis and for small molecules based drug screening.
Chapitres de livres sur le sujet "FLIMM method"
Petre, Anca, Didier Marty-Dessus, Laurent Berquez et Jean-Luc Franceschi. « FLIMM and FLAMM Methods ». Dans Dielectric Materials for Electrical Engineering, 251–70. Hoboken, NJ USA : John Wiley & Sons, Inc., 2013. http://dx.doi.org/10.1002/9781118557419.ch12.
Texte intégralKukk, Olga, Jeffrey Klarenbeek et Kees Jalink. « Time-Domain Fluorescence Lifetime Imaging of cAMP Levels with EPAC-Based FRET Sensors ». Dans cAMP Signaling, 105–16. New York, NY : Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2245-2_7.
Texte intégralMorton, Penny E., et Maddy Parsons. « Measuring FRET Using Time-Resolved FLIM ». Dans Methods in Molecular Biology, 403–13. Totowa, NJ : Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-207-6_27.
Texte intégralBonilla, Pedro Andrade, et Rebika Shrestha. « FLIM-FRET Protein-Protein Interaction Assay ». Dans Methods in Molecular Biology, 261–69. New York, NY : Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3822-4_19.
Texte intégralYoo, Tae Yeon, et Daniel J. Needleman. « Studying Kinetochores In Vivo Using FLIM-FRET ». Dans Methods in Molecular Biology, 169–86. New York, NY : Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3542-0_11.
Texte intégralWindgasse, Lukas, et Carsten Grashoff. « Multiplexed Molecular Tension Sensor Measurements Using PIE-FLIM ». Dans Methods in Molecular Biology, 221–37. New York, NY : Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2851-5_15.
Texte intégralLuyben, Thomas T., Jayant Rai, Bingyue Zhou, Hang Li et Kenichi Okamoto. « Two-Photon FRET/FLIM Imaging of Cerebral Neurons ». Dans Methods in Molecular Biology, 33–43. New York, NY : Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3810-1_4.
Texte intégralPandzic, Elvis, Renee Whan et Alex Macmillan. « Rapid FLIM Measurement of Membrane Tension Probe Flipper-TR ». Dans Methods in Molecular Biology, 257–83. New York, NY : Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1843-1_20.
Texte intégralBecker, Wolfgang, Samuel Frere et Inna Slutsky. « Recording Ca++ Transients in Neurons by TCSPC FLIM ». Dans Advanced Optical Methods for Brain Imaging, 103–10. Singapore : Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-9020-2_5.
Texte intégralLionetti, Maria Chiara, et Caterina Anna Maria La Porta. « FLIM-FRET Investigation of Heterogeneous Huntingtin Aggregation in HeLa Cells ». Dans Methods in Molecular Biology, 595–604. New York, NY : Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2597-2_36.
Texte intégralActes de conférences sur le sujet "FLIMM method"
Pham, C. D., V. Griseri et L. Berquez. « Space charge distribution detection by FLIMM and PEA method on electron beam irradiated dielectric films ». Dans 2009 IEEE Conference on Electrical Insulation and Dielectric Phenomena (CEIDP). IEEE, 2009. http://dx.doi.org/10.1109/ceidp.2009.5377748.
Texte intégralCerqueira, Matheus A., et Alexandre X. Falcão. « User-Assisted Design of a Neural Network for Brain Tumor Segmentation ». Dans Anais Estendidos da Conference on Graphics, Patterns and Images. Sociedade Brasileira de Computação - SBC, 2023. http://dx.doi.org/10.5753/sibgrapi.est.2023.27455.
Texte intégralLeiter, Nina, Maximilian Wohlschläger et Martin Versen. « Frequency-domain fluorescence lifetime imaging as method to analyze wood structures ». Dans CLEO : Applications and Technology. Washington, D.C. : Optica Publishing Group, 2022. http://dx.doi.org/10.1364/cleo_at.2022.jw3a.19.
Texte intégralNguyen, T. X., S. Bouchareb, V. Griseri et L. Berquez. « Post-electronic irradiation measurements by PEA and FLIMM methods on dielectric films ». Dans 2011 IEEE Conference on Electrical Insulation and Dielectric Phenomena - (CEIDP 2011). IEEE, 2011. http://dx.doi.org/10.1109/ceidp.2011.6232780.
Texte intégralKanaani, Salar, Mohammad Sadegh Helfroush, Habibollah Danyali et Mohammad Ali Kazemi. « Segmentation of skin lesions using an improved FLICM method ». Dans 2017 7th International Conference on Computer and Knowledge Engineering (ICCKE). IEEE, 2017. http://dx.doi.org/10.1109/iccke.2017.8167919.
Texte intégralJunek, J., et K. Zidek. « FLIM via RATS Method Using Single Pixel Camera ». Dans 3D Image Acquisition and Display : Technology, Perception and Applications. Washington, D.C. : OSA, 2020. http://dx.doi.org/10.1364/3d.2020.jw5c.1.
Texte intégralAlfonso-Garcia, Alba, Lisanne Kraft, Xiagnan Zhou, Julien Bec, Laura Marcu, Dongguang Wei, Shiro Urayama et Asha Cogdill. « Colorectal Polyp Assessment with Label-Free Fluorescence Lifetime Imaging ». Dans Clinical and Translational Biophotonics. Washington, D.C. : Optica Publishing Group, 2024. http://dx.doi.org/10.1364/translational.2024.tm3b.2.
Texte intégralHuang, YuHeng, Huaixin Guo et Tangsheng Chen. « Thermal conductivity of SiN flims with different thicknesses by 3ω method ». Dans Sixth Symposium on Novel Photoelectronic Detection Technology and Application, sous la direction de Huilin Jiang et Junhao Chu. SPIE, 2020. http://dx.doi.org/10.1117/12.2557726.
Texte intégralSchwarz, Jonas, Maximilian Wohlschläger, Nina Leiter, Veronika Auer, Michael Risse et Martin Versen. « Frequency Domain Fluorescence Lifetime Imaging Microscopy (FD-FLIM) analysis of Quercus robur samples for origin differentiation purposes ». Dans Applied Industrial Spectroscopy. Washington, D.C. : Optica Publishing Group, 2023. http://dx.doi.org/10.1364/ais.2023.jtu4a.10.
Texte intégralKomarova, A. D., S. D. Sinyushkina, A. M. Mozherov, I. S. Kritchenkov, S. P. Tunik, I. N. Druzhkova, V. I. Shcheslavskiy et M. V. Shirmanova. « IN VIVO STUDY OF THE OXYGEN AND METABOLIC STATUS OF TUMORS DURING ANTI-TUMOR THERAPY USING THE PLIM/FLIM METHOD ». Dans X Международная конференция молодых ученых : биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-185.
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