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1

Wang, Kai-Jin, Ying-Jun Zhang et Chong-Ren Yang. « Antioxidant phenolic constituents from Fagopyrum dibotrys ». Journal of Ethnopharmacology 99, no 2 (juin 2005) : 259–64. http://dx.doi.org/10.1016/j.jep.2005.02.029.

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Chen, Ming-Lin, Ya-Li You, Hui-Hui Wen et Ying Li. « The breeding system and reproductive ecology of the endangered plant Fagopyrum dibotrys (D. Don) Hara ». Bangladesh Journal of Botany 43, no 2 (19 janvier 2015) : 197–205. http://dx.doi.org/10.3329/bjb.v43i2.21673.

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Distyly has been confirmed in Fagopyrum dibotrys (D. Don) Hara. The species are dimorphic in style length, anther height, pollen size and number but not in flower diameter, pollen surface ornamentation and stigma papilla cells. In F. dibotrys populations, style-morph ratios vary from isoplethy (1L : 1S) to strongly L-biased, or they more often contain only the L-morph. Moreover, it was found that larger isoplethic populations of F. dibotrys could produce more seeds than smaller single-morph populations. F. dibotrys had a smaller population size, morph-biased populations, and lower seed setting, which could be the reasons for the decrease in its population DOI: http://dx.doi.org/10.3329/bjb.v43i2.21673 Bangladesh J. Bot. 43(2): 197-205, 2014 (September)
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Li, Xin, Jingling Liu, Qinxiang Chang, Ziyun Zhou, Ruilian Han et Zongsuo Liang. « Antioxidant and Antidiabetic Activity of Proanthocyanidins from Fagopyrum dibotrys ». Molecules 26, no 9 (21 avril 2021) : 2417. http://dx.doi.org/10.3390/molecules26092417.

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Proanthocyanidins are natural glycosidase inhibitors with excellent antioxidant activity. This study aims to search for a new source of proanthocyanidins for the prevention and treatment of type 2 diabetes with higher content and better activity and get their structure elucidated. First, the total proanthocyanidins contents (TOPCs), antioxidant activity, antidiabetic activity of seven common Polygonaceae plants were analyzed and compared. Then proanthocyanidins from the rhizome of Fagopyrum dibotrys were purified, and the detailed structure was comprehensively analyzed by ultraviolet visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), 13C nuclear magnetic resonance spectroscopy (13C NMR), reversed-phase high-performance liquid chromatography-electrospray mass spectrometry (RP-HPLC-ESI-MS), and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). The rhizome of F. dibotrys showed the highest TOPCs, the strongest antioxidant, and antidiabetic activities; the TOPCs, antioxidant and antidiabetic activities were all very significantly positively correlated. Proanthocyanidins purified from the rhizome of F. dibotrys showed better antidiabetic activity than grape seed proanthocyanidins (GsPs). Seventy-two proanthocyanidins from trimer to undecamer with a mean degree of polymerization (mDP) of about 5.02 ± 0.21 were identified with catechin and epicatechin as the dominant monomers. Conclusion: Proanthocyanidins are the main antioxidant and antidiabetic active substances of F. dibotrys and are expected to be developed into potential antioxidant and hypoglycemic products.
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Zhang, Jian, Zhi-Qi Yin, Peng Cao, You-Bin Li et Jin-Ao Duan. « A new flavonol derivative from Fagopyrum dibotrys ». Chemistry of Natural Compounds 44, no 6 (novembre 2008) : 701–3. http://dx.doi.org/10.1007/s10600-009-9197-1.

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Zhang, Chengcai, Yang Jiang, Changzheng Liu, Linyuan Shi, Jintong Li, Yan Zeng, Lanping Guo et Sheng Wang. « Identification of Medicinal Compounds of Fagopyri Dibotryis Rhizome from Different Origins and Its Varieties Using UPLC-MS/MS-Based Metabolomics ». Metabolites 12, no 9 (25 août 2022) : 790. http://dx.doi.org/10.3390/metabo12090790.

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Fagopyrum dibotrys, being native to southwest China, is widely distributed in Yunnan, Guizhou Provinces and Chongqing City. However, the quality of medicinal materials growing in different origins varies greatly, and cannot meet the market demand for high-quality F. dibotrys. In this study, 648 metabolites were identified, and phenolic compounds of F. dibotrys from different origins were clearly separated by principal component analysis (PCA). Our results suggested that the medicinal differences of F. dibotrys from different origins can be elucidated via the variations in the abundance of the phenolic and flavonoid compounds. We found that the epicatechin, total flavonoids and total tannin content in Yunnan Qujing (YQ) and Yunnan Kunming (YK) were higher than those in Chongqing Shizhu (CS), Chongqing Fuling (CF) and Guizhou Bijie (GB), suggesting that Yunnan Province can be considered as one of the areas that produce high-quality medicinal materials. Additionally, 1,6-di-O-galloyl-β-D-glucose, 2,3-di-O-galloyl-D-glucose and gallic acid could be used as ideal marker compounds for the quality control of F. dibotrys from different origins caused by metabolites, and the F. dibotrys planted in Yunnan Province is well worth exploiting.
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Liang, Chen, Jian-Ping Yuan, Tao Ding, Lv Yan, Lu Ling, Xin-Fu Zhou et Yue-Qin Zeng. « Neuroprotective Effect of Fagopyrum dibotrys Extract against Alzheimer’s Disease ». Evidence-Based Complementary and Alternative Medicine 2017 (2017) : 1–9. http://dx.doi.org/10.1155/2017/3294586.

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Accumulated evidence suggests that polyphenolic antioxidants present in herbs play important roles in prevention of AD; the molecular mechanisms behind neuroprotective actions rely on the phenols through different effects on the amyloid-aggregation pathway. Fagopyrum dibotrys is a traditional herbal medicine which contains high quantity phenols. In present study, we investigate the beneficial pharmacological actions of Fagopyrum dibotrys extract in the APP/PS1 transgenic mouse mode; meanwhile, effects of the FDE on the fibrillation and cytotoxicity of Aβ peptide were evaluated in vitro. After 9-month treatment, FDE exhibited multifunctional properties on Aβ-related pathologies, which cleaned Aβ deposits in the brain and decreased Aβ burden in the plasma, inhibited microhaemorrhage, and reduced reactive microglia in APP/PS1 transgenic mice and also promoted Aβ fibrils disaggregation and inhibited neurotoxicity induced by Aβ in SH-SY5Y cells. These results highlighted that FDE is an AD type pathology modulator with therapeutic potential against AD.
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Zhang, Xiang, Yu Cao, Jinhua Li, Ailin Liu, Haibo Liu et Linfang Huang. « Neuraminidase Inhibitory Activity and Constituent Characterization of Fagopyrum dibotrys ». Molecules 22, no 11 (18 novembre 2017) : 1998. http://dx.doi.org/10.3390/molecules22111998.

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Bai, Zheng Zhong, Xiao Hui Zhang, Li Jiang Xuan et Feng Kui Mo. « A phenolic glycoside from Fagopyrum dibotrys (D. Don) Hara ». Chinese Chemical Letters 18, no 9 (septembre 2007) : 1087–88. http://dx.doi.org/10.1016/j.cclet.2007.07.033.

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Mitishev, Alexander Vladimirovich, Anastasia Sergeevna Fednina, Evgeniy Evgenievich Kurdyukov et Marina Igorevna Ulanova. « Some species of the genus Fagopyrum : chemical composition, pharmacological effects, prospects of use ». chemistry of plant raw material, no 1 (7 février 2024) : 31–56. http://dx.doi.org/10.14258/jcprm.20240112933.

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This review analyzes and summarizes the information of research by domestic and foreign scientists on the chemical composition, pharmacological activity of metabolites of plants of the genus Fagopyrum. The genus Fagopyrum, a member of the Polygonaceae family, includes 15 species, most of them are cultivated on an industrial scale on the territory of the Russian Federation, China, India, Indonesia and North American countries. The greatest interest in medicine and pharmacy is represented by four types of buckwheat: seed buckwheat (Fagopyrum esculentum) and Tatar buckwheat (Fagopyrum tataricum), perennial buckwheat (Fagopyrum dibotrys) and red-stem buckwheat (Fagopyrum rubricaulis). These types are widely used in traditional medicine of China, Tibet, the Russian Federation, for the treatment of lung diseases, skin, edematous syndromes and infectious diseases. Currently, more than 150 compounds related to flavonoids, phenylpropanoids, tannins, phagopyritols, triterpenoids, steroids, fatty acids, essential oils, alkaloids, anthraquinones, coumarins, vitamins, amino acids, macro- and microelements have been isolated and identified from the studied buckwheat species. Extracts and isolated individual compounds have a wide range of pharmacological effects: antitumor, antioxidant, anti-inflammatory, hepatoprotective, hypoglycemic, antiallergic, antibacterial, angioprotective and adaptogenic. The information given in the review indicates that plants of the genus Fagopyrum are promising for further study and use in various industries.
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Guo, Yihui, Xinke Tang, Siren Hu, Kaiqin Li, Meiliang Zhou et Gao Jian. « Steptomyces fagopyri sp. nov., a novel actinomycete isolated from rhizospheric soil of Fagopyrum dibotrys ». International Journal of Systematic and Evolutionary Microbiology 70, no 12 (1 décembre 2020) : 6437–43. http://dx.doi.org/10.1099/ijsem.0.004555.

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A novel actinomycete, designated strain QMT-28T, was isolated from rhizosphere soil of Fagopyrum dibotrys collected from Shuangfeng, Hunan Province, PR China. Strain QMT-28T grew well on International Streptomyces Project series media and formed well-developed, branched substrate hyphae and aerial mycelium that differentiated into loose spiral spore chains consisting of cylindrical spores with smooth surfaces. The diagnostic diamino acid was ll-diaminopimelic acid and the whole-cell sugars were galactose and glucose. The predominant fatty acids were C18 : 1 cis9, summed feature 6 (C18 : 2 cis 9,12/C18 : 0 a) and C16 : 0. The polar lipids included diphosphatidylglycerol, hydroxy phospatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, phospholipids of unknown structure containing glucosamine and several unidentified phospholipids. The major menaquinones were MK-9, MK-9(H2), MK-9(H4), MK-9(H6) and MK-9(H8). The genome size of strain QMT-28T was about 8.7 Mbp with a G+C content of 71.2 mol%. Phylogenetic analysis showed that the novel strain was closely related to Streptomyces olivochromogenes DSM 40451T (99.5 % similarity), Streptomyces mirabilis NBRC 13450T (98.9 %), Streptomyces kanamyceticus NBRC 13414T (98.9 %), Streptomyces kaempferi I37T (98.9 %) and Streptomyces arcticus ZLN234T (98.8 %). However, the average nucleotide identity values, the digital DNA–DNA hybridization values and the multilocus sequence analysis evolutionary distances between this strain and closely related strains showed that it belonged to a distinct species. In addition, these results were also supported by differences in the phenotypic characteristics between QMT-28T and five closely related type strains. Consequently, strain QMT-28T should represent a novel species of the genus Streptomyces , with the suggested name Streptomyces fagopyri sp. nov. The type strain is QMT-28T (=CICC 24808T=JCM 33796T).
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游, 亚丽. « Study on Morphology Anatomical Characters of Fagopyrum dibotrys and F. esculentum ». Botanical Research 01, no 02 (2012) : 41–45. http://dx.doi.org/10.12677/br.2012.12007.

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Zhang, Yan, et Chen Chen. « The complete chloroplast genome sequence of the medicinal plant Fagopyrum dibotrys (Polygonaceae) ». Mitochondrial DNA Part B 3, no 2 (3 juillet 2018) : 1087–89. http://dx.doi.org/10.1080/23802359.2018.1483761.

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Min, Daozhang, Wei Shi, Mohammad Mehdi Dehshiri, Yuting Gou, Wei Li, Kaixuan Zhang, Meiliang Zhou et Bo Li. « The molecular phylogenetic position of Harpagocarpus (Polygonaceae) sheds new light on the infrageneric classification of Fagopyrum ». PhytoKeys 220 (27 février 2023) : 109–26. http://dx.doi.org/10.3897/phytokeys.220.97667.

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In the context of the molecular phylogeny of Polygonaceae, the phylogenetic positions of most genera and their relationships have been resolved. However, the monotypic genus Harpagocarpus has never been included in any published molecular phylogenetic studies. In the present study, we adopt a two-step approach to confirm the phylogenetic placement of Harpagocarpus using two datasets: (1) a concatenated dataset of three chloroplast DNA (cpDNA) regions (matK, rbcL and trnL-F) for Polygonaceae and (2) a combined cpDNA dataset of five sequences (accD, matK, psbA-trnH, rbcL and trnL-F) for Fagopyrum. Our analyses confirm the previous hypothesis based on morphological, anatomical and palynological investigations that Harpagocarpus is congeneric with Fagopyrum and further reveal that H. snowdenii (= F. snowdenii) is sister to the woody buckwheat F. tibeticum. Within Fagopyrum, three highly supported clades were discovered and the first sectional classification was proposed to accommodate them: sect. Fagopyrum comprises the two domesticated common buckwheat (F. esculentum and F. tataricum) and their wild relatives (F. esculentum subsp. ancestrale, F. homotropicum and F. dibotrys) which are characterised by having large corymbose inflorescences and achenes greatly exceeding the perianth; sect. Tibeticum, including F. snowdenii and F. tibeticum, is characterised by the achene having appurtenances along the ribs, greatly exceeding the perianth and the perianth accrescent in fruit; sect. Urophyllum contains all other species of which the achenes were completely enclosed in the perianth. This study is very helpful to understand the phylogeny of the Fagopyrum and sheds light on the future study of taxonomy, biogeography, diversification and character evolution of the genus.
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CHEN, Cai-Xia, Si-Ran CUI, Yan-Hua JIANG et Ai-Lian LI. « Callus Induction and Plant Regeneration of Fagopyrum dibotrys(D.Don) Hara,An Important Medicinal Plant ». Plant Science Journal 30, no 3 (2012) : 315. http://dx.doi.org/10.3724/sp.j.1142.2012.30315.

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亚丽, 游. « 金荞麦和荞麦的形态解剖学特征研究Study on Morphology Anatomical Characters of Fagopyrum dibotrys and F. esculentum ». Botanical Research 01, no 02 (2012) : 41–45. http://dx.doi.org/10.4236/br.2012.12007.

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Feng, Shengbao, Lixia Song, Yuancai Liu, Fuli Lai, Gang Zuo, Guangyuan He, Mingjie Chen et Dejian Huang. « Hypoglycemic Activities of Commonly-Used Traditional Chinese Herbs ». American Journal of Chinese Medicine 41, no 04 (janvier 2013) : 849–64. http://dx.doi.org/10.1142/s0192415x13500572.

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The α-amylase and α-glucosidase inhibition activity of 92 Traditional Chinese Medicinal (TCM) herbs, which are permitted to be used as food ingredients, were evaluated using the high throughput assay developed in our laboratory. Among these herbs, twenty-seven of them possessed significant α-amylase inhibition activities ranging from 2.4 to 349.2 μmol AE/g (AE = acarbose equivalent) with inhibition concentrations at 50% inhibition (IC50) from 16.0 to 2342.2 μg/mL, respectively. In addition, they showed α-glucosidase inhibition activities ranging from 0.5 to 31.6 μmolAE/g (IC50 from 49.0 to 3385.5 μg/mL). The extracts of Rhizoma fagopyri dibotryis (Jīnqiáomài), Rosa rugosa (Méiguīhuā), Caulis polygoni multiflori (Shǒuwūténg), Fructus amomi (Shārén), Rhizoma alpiniae officinarum (Gāoliángjiāng), Folium ginkgo (Yínxìngyè) and Cortex cinnamomi (Ròuguì) showed the better inhibitory activities against both α-amylase and α-glucosidase. Our results illustrated that these food grade herbs are potent natural hypoglycemic agents and can be used as active ingredients for low glycemic index food production or TCM herbal formulations for controlling hyperglycemia.
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Si, Fuchun, Wenyu Zhao, Gao Si et Xuejie Song. « Abstract 4720 : Effects of removing heat and nourishing yin prescription on malignant phenotype and PI3K/AKT signaling pathway of human esophageal carcinoma cells ». Cancer Research 84, no 6_Supplement (22 mars 2024) : 4720. http://dx.doi.org/10.1158/1538-7445.am2024-4720.

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Abstract Introduction: Esophageal carcinoma (EC) ranks eighth in the incidence of malignant tumors and the sixth mortality worldwide. This study aims to screen out the Chinese herbs with strong inhibitory effects on the proliferation of four kinds of EC cells EC9706, EC-1, TE-1 and Eca109 from 366 Chinese herbs produced in Henan Province, and study the effect of composed Removing Heat and Nourshing Yin Prescription(RHNYP) on the proliferation, migration, invasion and PI3K/AKT signaling pathway of four EC cells. Methods: MTT and orthogonal design was applied to screen out the best effective Chinese herbs and determine the optimal dosage ratio of prescription on EC9706, EC-1, TE-1, Eca109 cells from 366 Chinese herbs alcohol extracts, the effects of this prescription on the proliferation, migration, invasion, clone formation, cell cycle and apoptosis of four EC cells were investigated by MTT, RTCA, soft agarose colony forming test, flow cytometry and western blot analysis. Then exploring the effects of RHNYP on EC related signaling pathways and key genes expression by western blot and RT-PCR assay. Results: Rhizoma Fagopyri Dibotryis(Jinqiaomai), Rhizoma Anemarrhenae(Zhimu), Asparagus Cochinchinensis(Tiandong), and Carpesium Abrolanoides Linne(Heshi) were screened out and composed Removing Heat and Nourshing Yin Prescription(RHNYP) according to their efficacy and orthogonal design. RHNYP could obviously inhibit the proliferation of EC9706, TE-1, EC-1, ECa109 cells with dose-effect and time-dependent manner. IC50 values of Jinqiaomai, Zhimu, Tiandong, Heshi in RHNYP for EC9706 cell were 27.639μg/ml, 40.015μg/ml, 9.044μg/ml, 11.208μg/ml respectively; for EC-1 cell were 24.575μg/ml, 56.991μg/ml, 9.361μg/ml, 13.584μg/ml respectively; for TE-1 cell were 23.534μg/ml, 25.862μg/ml, 6.788μg/ml, 8.911μg/ml respectively; for ECa109 cell were 33.990μg/ml, 33.604μg/ml, 19.873μg/ml, 15.227μg/ml respectively. RHNYP could effectively inhibit the proliferation, migration, invasion, cloning ability, cell cycle and reduce apoptosis of four EC cells. PI3K/AKT/mTOR pathway had closely relationship with the occurrence and development of EC and RHNYP could effectively regulate the proteins expression on the pathway. CDCK3, KIF4A and RAD51AP1 genes were negatively correlated with the overall survival of EC patients and RHNYP could significantly inhibit their expression in four EC cells. Conclusion: RHNYP can effectively inhibit EC cell proliferation, migration, invasion and cell cycle, induce apoptosis, which may be related to the regulation of PI3K/AKT/mTOR pathway. Citation Format: Fuchun Si, Wenyu Zhao, Gao Si, Xuejie Song. Effects of removing heat and nourishing yin prescription on malignant phenotype and PI3K/AKT signaling pathway of human esophageal carcinoma cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 4720.
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« Fagopyrum dibotrys ». CABI Compendium CABI Compendium (7 janvier 2022). http://dx.doi.org/10.1079/cabicompendium.114372.

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Zhang, Le-Le, Yan He, Feiya Sheng, Ying-Fan Hu, Yu Song, Wei Li, Jiarong Chen, Jinming Zhang et Liang Zou. « Towards a better understanding of Fagopyrum dibotrys : a systematic review ». Chinese Medicine 16, no 1 (16 septembre 2021). http://dx.doi.org/10.1186/s13020-021-00498-z.

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AbstractFagopyrum dibotrys (F. dibotrys) (D.Don) H.Hara is a well-known edible herbal medicine in Asian countries. It has been widely used for the treatment of lung diseases, swelling, etc., and is also an important part of many Chinese medicine prescriptions. At present, more than 100 compounds have been isolated and identified from F. dibotrys, and these compounds can be primarily divided into flavonoids, phenols, terpenes, steroids, and fatty acids. Flavonoids and phenolic compounds are considered to be the main active ingredients of F. dibotrys. Previous pharmacological studies have shown that F. dibotrys possesses anti-inflammatory, anti-cancer, anti-oxidant, anti-bacterial, and anti-diabetic activities. Additional studies on functional genes have led to a better understanding of the metabolic pathways and regulatory factors related with the flavonoid active ingredients in F. dibotrys. In this paper, we systemically reviewed the research advances on the phytochemistry and pharmacology of F. dibotrys, as well as the functional genes related to the synthesis of active ingredients, aiming to promote the development and utilization of F. dibotrys.
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Ma, Nan, Dengpan Yin, Ying Liu, Ziyong Gao, Yu Cao, Tongtong Chen, Ziyi Huang, Qiaojun Jia et Dekai Wang. « Succession of endophytic fungi and rhizosphere soil fungi and their correlation with secondary metabolites in Fagopyrum dibotrys ». Frontiers in Microbiology 14 (1 août 2023). http://dx.doi.org/10.3389/fmicb.2023.1220431.

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Golden buckwheat (Fagopyrum dibotrys, also known as F. acutatum) is a traditional edible herbal medicinal plant with a large number of secondary metabolites and is considered to be a source of therapeutic compounds. Different ecological environments have a significant impact on their compound content and medicinal effects. However, little is known about the interactions between soil physicochemical properties, the rhizosphere, endophytic fungal communities, and secondary metabolites in F. dibotrys. In this study, the rhizosphere soil and endophytic fungal communities of F. dibotrys in five different ecological regions in China were identified based on high-throughput sequencing methods. The correlations between soil physicochemical properties, active components (total saponins, total flavonoids, proanthocyanidin, and epicatechin), and endophytic and rhizosphere soil fungi of F. dibotrys were analyzed. The results showed that soil pH, soil N, OM, and P were significantly correlated with the active components of F. dibotrys. Among them, epicatechin, proanthocyanidin, and total saponins were significantly positively correlated with soil pH, while proanthocyanidin content was significantly positively correlated with STN, SAN, and OM in soil, and total flavone content was significantly positively correlated with P in soil. In soil microbes, Mortierella, Trechispora, Exophiala, Ascomycota_unclassified, Auricularia, Plectosphaerella, Mycena, Fungi_unclassified, Agaricomycetes_unclassified, Coprinellus, and Pseudaleuria were significantly related to key secondary metabolites of F. dibotrys. Diaporthe and Meripilaceae_unclassified were significantly related to key secondary metabolites in the rhizome. This study presents a new opportunity to deeply understand soil-plant-fungal symbioses and secondary metabolites in F. dibotrys, as well as provides a scientific basis for using biological fertilization strategies to improve the quality of F. dibotrys.
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Guo, Yihui, Xinke Tang, Siren Hu, Kaiqin Li, Meiliang Zhou et Gao Jian. « Erratum : Steptomyces fagopyri sp. nov., a novel actinomycete isolated from rhizospheric soil of Fagopyrum dibotrys ». International Journal of Systematic and Evolutionary Microbiology 71, no 9 (20 septembre 2021). http://dx.doi.org/10.1099/ijsem.0.005029.

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Jia, C. F., et A. L. Li. « Effect of gamma radiation on mutant induction of Fagopyrum dibotrys Hara ». Photosynthetica 46, no 3 (1 septembre 2008). http://dx.doi.org/10.1007/s11099-008-0066-0.

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Fan, Yu, Ya’nan Jin, Mengqi Ding, Yu Tang, Jianping Cheng, Kaixuan Zhang et Meiliang Zhou. « The Complete Chloroplast Genome Sequences of Eight Fagopyrum Species : Insights Into Genome Evolution and Phylogenetic Relationships ». Frontiers in Plant Science 12 (15 décembre 2021). http://dx.doi.org/10.3389/fpls.2021.799904.

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Buckwheat (Fagopyrum genus, Polygonaceae), is an annual or perennial, herbaceous or semi-shrub dicotyledonous plant. There are mainly three cultivated buckwheat species, common buckwheat (Fagopyrum esculentum) is widely cultivated in Asia, Europe, and America, while Tartary buckwheat (F. tataricum) and F. cymosum (also known as F. dibotrys) are mainly cultivated in China. The genus Fagopyrum is taxonomically confusing due to the complex phenotypes of different Fagopyrum species. In this study, the chloroplast (cp) genomes of three Fagopyrum species, F. longistylum, F. leptopodum, F. urophyllum, were sequenced, and five published cp genomes of Fagopyrum were retrieved for comparative analyses. We determined the sequence differentiation, repeated sequences of the cp genomes, and the phylogeny of Fagopyrum species. The eight cp genomes ranged, gene number, gene order, and GC content were presented. Most of variations of Fagopyrum species cp genomes existed in the LSC and SSC regions. Among eight Fagopyrum chloroplast genomes, six variable regions (ndhF-rpl32, trnS-trnG, trnC, trnE-trnT, psbD, and trnV) were detected as promising DNA barcodes. In addition, a total of 66 different SSR (simple sequence repeats) types were found in the eight Fagopyrum species, ranging from 8 to 16 bp. Interestingly, many SSRs showed significant differences especially in some photosystem genes, which provided valuable information for understanding the differences in light adaptation among different Fagopyrum species. Genus Fagopyrum has shown a typical branch that is distinguished from the Rumex, Rheum, and Reynoutria, which supports the unique taxonomic status in Fagopyrum among the Polygonaceae. In addition, phylogenetic analysis based on the cp genomes strongly supported the division of eight Fagopyrum species into two independent evolutionary directions, suggesting that the separation of cymosum group and urophyllum group may be earlier than the flower type differentiation in Fagopyrum plants. The results of the chloroplast-based phylogenetic tree were further supported by the matK and Internal Transcribed Spacer (ITS) sequences of 17 Fagopyrum species, which may help to further anchor the taxonomic status of other members in the urophyllum group in Fagopyrum. This study provides valuable information and high-quality cp genomes for identifying species and evolutionary analysis for future Fagopyrum research.
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Guo, Xinwei, Zuliang Luo, Min Zhang, Linfang Huang, Hui Wang, Yuting Li, Xu Qiao, Ailian Li et Bin Wu. « The spatiotemporal regulations of epicatechin biosynthesis under normal flowering and the continuous inflorescence removal treatment in Fagopyrum dibotrys ». BMC Plant Biology 22, no 1 (29 juillet 2022). http://dx.doi.org/10.1186/s12870-022-03761-z.

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Abstract Background Flowering is a critical physiological change that interferes with not only biomass yield but also secondary metabolism, such as the biosynthesis of flavonoids, in rhizome/root plants. The continuous inflorescence removal (CIR) treatment is frequently conducted to weaken this effect. Fagopyrum dibotrys (D.Don) H.Hara (Golden buckwheat) is a kind of rhizome medicinal plant rich in flavonoids and is widely used for the treatment of lung diseases. The CIR treatment is usually conducted in F. dibotrys because of its excessive reproductive growth. To uncover the molecular mechanisms, comprehensive analysis was performed using metabolome and transcriptome data obtained from normally bloomed and the CIR treated plants. Results Metabolome results demonstrated that in the rhizomes of F. dibotrys, its bioactive compound called epicatechin has higher amount than most of the detected precursors. Compared with the normally bloomed plants, the level of epicatechin in the rhizomes of the CIR group increased by 25% at the withering stage. Based on 96 samples of the control and the CIR groups at 4 flowering stages for 4 tissues, RNA-Seq results revealed a 3 ~ 5 times upregulations of all the key enzyme genes involved in the biosynthesis of epicatechin in both time (from the bud stage to the withering stage) and spatial dimensions (from the top of branch to rhizome) under the CIR treatment compared to normal flowering. Integrated analysis of LC–MS/MS and transcriptome revealed the key roles of several key enzyme genes besides anthocyanidin reductase (ANR). A total of 93 transcription factors were identified to co-expressed with the genes in epicatechin biosynthetic pathway. The flowering activator SQUAMOSA promoter-binding protein like (SPLs) exhibited opposite spatiotemporal expression patterns to that of the epicatechin pathway genes; SPL3 could significantly co-express with all the key enzyme genes rather than the flowering repressor DELLA. Weighted gene co-expression network analysis (WGCNA) further confirmed the correlations among chalcone synthases (CHSs), chalcone isomerases (CHIs), ANRs, SPLs and other transcription factors. Conclusions SPL3 might dominantly mediate the effect of normal flowering and the CIR treatment on the biosynthesis of epicatechin in rhizomes mainly through the negative regulations of its key enzyme genes including CHS, CHI and ANR.
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Chenglei, LI. « Molecular cloning and prokaryotic expression of phenylalanine ammonia- lyase gene FdPAL from Fagopyrum dibotrys ». China Journal of Chinese Materia Medica, 1 décembre 2011. http://dx.doi.org/10.4268/cjcmm20112305.

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Wang, Xumei, Tao Zhou, Guoqing Bai et Yuemei Zhao. « Complete chloroplast genome sequence of Fagopyrum dibotrys : genome features, comparative analysis and phylogenetic relationships ». Scientific Reports 8, no 1 (17 août 2018). http://dx.doi.org/10.1038/s41598-018-30398-6.

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Huo, Ziting, Wenbo Xu, Huijun Guo, Peng Yang, Qianwen Zhang, Xu Lu et Long Wang. « The complete chloroplast genome of Persicaria perfoliata and comparative analysis with Four Medicinal Plants of Polygonaceae ». Genome, 16 mai 2022. http://dx.doi.org/10.1139/gen-2021-0085.

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Polygonaceae is a large family of medicinal herbs that includes many species used as traditional Chinese medicine, such as Persicaria perfoliate. Here, we sequenced the complete cp genome of P. perfoliata using Illumina sequencing technology with the purpose to provide a method to facilitate accurate identification. After being annotated, the cp genome of P. perfoliata was compared with Fagopyrum tataricum, Persicaria chinensis, Fagopyrum dibotrys and Fallopia multiflora. The complete cp genome of P. perfoliata is 160,730 bp in length, containing a small single copy (SSC) region of 12,927 bp, a large single copy (LSC) region of 85,433 bp and a pair of inverted repeats (IR) regions of 62,370 bp. A total of 131 genes were annotated, including eight rRNA genes, 34 tRNA genes and 84 protein-coding genes. Forty-two simple sequence repeats and fifty-five repeat sequences were identified. Mutational hot spots analyses indicated that five genes (matK, ndhF, ccsA, cemA, rpl20) could be selected as candidates for molecular markers. Moreover, phylogenetic analysis showed that all the Polygonaceae species formed a monophyletic clade, and P. perfoliata showed the closest relationship with P. chinense. The study provides valuable molecular information to accurately identify P. perfoliata and assist in its development and application.
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Lan, J. X., A. L. Li et C. X. Chen. « Effect of transient accumulation of anthocyanin on leaf development and photoprotection of Fagopyrum dibotrys mutant ». Biologia plantarum 55, no 4 (1 décembre 2011). http://dx.doi.org/10.1007/s10535-011-0184-6.

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Zhang, Dan, Yongfang Xu, Hang Chen, Da Wang, Zuotao Geng, Yuanli Chen, Yan Chen et al. « Fagopyrum dibotrys extract alleviates hepatic steatosis and insulin resistance, and alters autophagy and gut microbiota diversity in mouse models of high-fat diet-induced non-alcoholic fatty liver disease ». Frontiers in Nutrition 9 (14 novembre 2022). http://dx.doi.org/10.3389/fnut.2022.993501.

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Non-alcoholic fatty liver disease (NAFLD) is a major global health concern with increasing prevalence, with a lack of currently available effective treatment options; thus, the investigation of novel therapeutic approaches is necessary. The study aimed to investigate the outcomes and mechanisms of action of Fagopyrum dibotrys extract (FDE) in a high-fat diet (HFD)-induced mouse model of obesity. The findings showed that FDE supplementation attenuated glucose tolerance, insulin resistance (IR), hepatic steatosis, and abnormal lipid metabolism. In addition, FDE also promoted autophagic activity and inhibited the phosphorylation of transcription factor EB in HFD-fed mice. Furthermore, gut microbiota characterization via 16S rRNA sequencing revealed that the supplementation of FDE increased Bacteroidetes and Verrucomicrobia populations while decreased Firmicutes, thus modifying the gut microbiome. FDE also increased the relative abundance of Akkermansia. Our findings suggest that FDE may protect against HFD-induced NAFLD by activating autophagy and alleviating dysbiosis in the gut microbiome. FDE may be beneficial as a nutraceutical treatment for NAFLD.
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Yifeng, ZHANG. « Influences of enhanced UV-B radiation and drought stress on biomass accumulation and allocation of Fagopyrum dibotrys ». China Journal of Chinese Materia Medica, 1 août 2011. http://dx.doi.org/10.4268/cjcmm20111505.

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Zhang, Min, Xinke Zhang, Jin Pei, Baolin Guo, Guoshuai Zhang, Minhui Li et Linfang Huang. « Identification of phytochemical compounds of Fagopyrum dibotrys and their targets by metabolomics, network pharmacology and molecular docking studies ». Heliyon, mars 2023, e14029. http://dx.doi.org/10.1016/j.heliyon.2023.e14029.

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Hu, Yingfan, Xiaomin Liu, Yu Song, Yan Zhang, Wei Li, Lele Zhang, Anqi Wang, Qian Su, Zhiyong Yang et Liang Zou. « Exploring the anti‐inflammatory ingredients and potential of golden buckwheat (Fagopyrum dibotrys) on the TLR4/NLRP3 pathway in acute lung injury ». Food Science & ; Nutrition, 26 avril 2024. http://dx.doi.org/10.1002/fsn3.4193.

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AbstractGolden buckwheat, also called Fagopyrum dibotrys (D. Don) H. Hara, is a plant of the genus Buckwheat in the buckwheat family. The aim of this study was to screen the bioactive ingredients of golden buckwheat extract and investigate the protective effect on acute lung injury (ALI). The ethyl acetate extract (EAE) was identified as the active fraction in LPS‐induced RAW264.7 cells, with gallic acid, proanthocyanidin B2, and epicatechin at 0.0563%, 0.3707%, and 0.3868%, respectively. At the same time, 20 compounds (mainly flavonoids and organic acids) were identified by UPLC‐Q‐Exactive Orbitrap‐HRMS in EAE. Furthermore, the EAE reduced lung histopathology scores in mice with ALI, decreased the dry‐to‐wet weight ratio of lung tissue, and significantly inhibited the concentrations of IL‐1β, TNFα, and IL‐6 in bronchoalveolar lavage fluid (BALF). It also reduced the number of leukocytes, decreased the activity of MPO in lung tissue, and inhibited the levels of TLR4/NLRP3 pathway mRNA and protein in lung tissue. Our study indicated that golden buckwheat as a source of functional food prevents or treats associated lung diseases by modulating the activation of the TLR4/NLRP3 signaling pathway.
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Chen, Caixia, et Ailian Li. « Transcriptome Analysis of Differentially Expressed Genes Involved in Proanthocyanidin Accumulation in the Rhizomes of Fagopyrum dibotrys and an Irradiation-Induced Mutant ». Frontiers in Physiology 7 (18 mars 2016). http://dx.doi.org/10.3389/fphys.2016.00100.

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Chen, Zhaojun, Guotao Dai, Xian Wu, Lina Li, Yujie Tian et Lulin Tan. « Protective effects of Fagopyrum dibotrys on oxidized oil-induced oxidative stress, intestinal barrier impairment, and altered cecal microbiota in broiler chickens ». Poultry Science, janvier 2023, 102472. http://dx.doi.org/10.1016/j.psj.2022.102472.

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Li, Zhiyu, Yunying Xiao, Kexuan Zhou, Xin Jin, Wan Li, Wanzhi Li, Lu Zhang, Jin Wang, Rongping Hu et Lijin Lin. « Water extract of Fagopyrum dibotrys (D. Don) Hara straw increases selenium accumulation in peach seedlings under selenium-contaminated soil ». International Journal of Phytoremediation, 8 septembre 2023, 1–10. http://dx.doi.org/10.1080/15226514.2023.2255287.

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Caixia Chen,. « In vitro propagation and quality evaluation of long-term micro-propagated and conventionally grown Fagopyrum dibotrys Hara mutant, an important medicinal plant ». Journal of Medicinal Plants Research 6, no 15 (23 mai 2012). http://dx.doi.org/10.5897/jmpr11.1508.

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Wang, Xiaojiang, Yukun Jiao, Haiyan Zhu, Yan Lu et Daofeng Chen. « Exploring the anticomplement components from Fagopyrum dibotrys for the treatment of H1N1-induced acute lung injury by UPLC-Triple-TOF-MS/MS ». Journal of Pharmaceutical and Biomedical Analysis, novembre 2022, 115158. http://dx.doi.org/10.1016/j.jpba.2022.115158.

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Geng, Qi, Bin Liu, Zhiwen Cao, Li Li, Peipei Lu, Lin Lin, Lan Yan et Cheng Lu. « Ethnobotany, phytochemistry and pharmacological properties of Fagopyri Dibotryis Rhizoma : A review ». Frontiers in Pharmacology 14 (6 mars 2023). http://dx.doi.org/10.3389/fphar.2023.1095554.

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Fagopyri Dibotryis Rhizoma (FDR) is an effective Chinese herbal medicine with a long history of use in China. FDR is effective in heat clearing and detoxifying, promotion of blood circulation, relieving carbuncles, dispelling wind, and removing dampness. Its seeds also have high nutritional value, are rich in protein, and contain a variety of mineral elements and vitamins. Therefore, FDR is considered a natural product with medical and economic benefits, and its chemical composition and pharmacological activity are of interest to scientists. The current review provides an overview of the available scientific information on FDR, particularly its botany, chemical constituents, and pharmacological activities. Various sources of valid and comprehensive relevant information were consulted, including the China National Knowledge Infrastructure, Web of Science, and PubMed. Among the keywords used were “Fagopyri Dibotryis Rhizoma”, “botanical features”, “chemical composition”, and “pharmacological activity” in combination. Various ailments are treated with FDR, such as diabetes, tumor, sore throat, headache, indigestion, abdominal distension, dysentery, boils, carbuncles, and rheumatism. FDR is rich in organic acids, tannins, flavonoids, steroids, and triterpenoids. Experiments performed in vitro and in vivo showed that FDR extracts or fractions had a wide range of pharmacological activities, including antitumor, anti-inflammatory, immunomodulatory, antioxidant, antimicrobial, and antidiabetic. The current review provides an integrative perspective on the botany, phytochemistry and pharmacological activities of FDR. FDR may be used as a medicine and food. Based on its chemical composition and pharmacological effects, the main active ingredients of FDR are organic acids, tannins, and flavonoids, and it has obvious antitumor pharmacological activity against a variety of malignant tumors. Therefore, FDR is worthy of further study and application as a potential antitumor drug.
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« Study on biosynthesis of silver nanoparticles using Fagopyri Dibotryis Rhizoma extract and optimization of synthesis conditions ». China Journal of Chinese Materia Medica, 1 mai 2014. http://dx.doi.org/10.4268/cjcmm20140911.

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