Littérature scientifique sur le sujet « F-11 cells »
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Articles de revues sur le sujet "F-11 cells"
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Sgambato, Antonella, Valentina Pastori, Laura Russo, Simone Vesentini, Marzia Lecchi et Laura Cipolla. « Neoglycosylated Collagen : Effect on Neuroblastoma F-11 Cell Lines ». Molecules 25, no 19 (23 septembre 2020) : 4361. http://dx.doi.org/10.3390/molecules25194361.
Texte intégralRésumé :
The regeneration of the nervous system is a challenging task. Currently, regenerative medicine approaches that exploit nature-inspired cues are being studied and hold great promise. The possibility to use protein-based matrices functionalized with small oligo- and monosaccharides is of interest since these can be finely tuned to better mimic the native environment. Collagen has been selected as a promising material that has the potential to be further tailored to incorporate carbohydrates in order to drive cell behavior towards neuroregeneration. Indeed, the grafting of carbohydrates to collagen 2D matrices is proved to enhance its biological significance. In the present study, collagen 2D matrices were grafted with different carbohydrate epitopes, and their potential to drive F-11 neuroblastoma cells towards neuronal differentiation was evaluated. Collagen functionalized with α-glucosides was able to differentiate neuroblastoma cells into functional neurons, while sialyl α-(2→6)-galactosides stimulated cell proliferation.
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Breen, Shawn M., Nebojsa Andric, Tai Ping, Fang Xie, Stefan Offermans, Jan A. Gossen et Mario Ascoli. « Ovulation Involves the Luteinizing Hormone-Dependent Activation of Gq/11 in Granulosa Cells ». Molecular Endocrinology 27, no 9 (1 septembre 2013) : 1483–91. http://dx.doi.org/10.1210/me.2013-1130.
Texte intégralRésumé :
The LH receptor (LHR) activates several families of heterotrimeric G proteins, but only the activation of Gs and subsequent generation of cAMP are universally accepted as important mediators of LH actions. To examine the involvement of the Gq/11 family on the actions of LH, we crossed Cyp19Cre and Gαqf/f;Gα11−/− mice to generate mice with a granulosa cell-specific deletion of Gαq in the context of a global deletion of Gα11. Granulosa cells from Gαqf/f;Gα11−/−;Cre+ mice have barely detectable levels of Gαq/11, have a normal complement of LHR, and respond to LHR activation with a transient increase in cAMP accumulation, but they fail to respond with increased inositol phosphate accumulation, an index of the activation of Gαq/11. The LHR-provoked resumption of meiosis, cumulus expansion, and luteinization are normal. However, the Gαqf/f;Gα11−/−;Cre+ mice display severe subfertility because many of the oocytes destined for ovulation become entrapped in preovulatory follicles or corpora lutea. Because follicular rupture is known to be dependent on the expression of the progesterone receptor (Pgr), we examined the LHR-induced expression of Pgr and 4 of its target genes (Adamts-1, Ctsl1, Edn2, and Prkg2). These actions of the LHR were impaired in the ovaries of the Gαqf/f;Gα11−/−;Cre+ mice. We conclude that the defect in follicular rupture is secondary to the failure of the LHR to fully induce the expression of the Pgr. This is the first conclusive evidence for the physiological importance of the activation of Gq/11 by the LHR and for the involvement of Gαq/11 in ovulation.
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Pastori, Valentina, Alessia D’Aloia, Stefania Blasa et Marzia Lecchi. « Serum-deprived differentiated neuroblastoma F-11 cells express functional dorsal root ganglion neuron properties ». PeerJ 7 (30 octobre 2019) : e7951. http://dx.doi.org/10.7717/peerj.7951.
Texte intégralRésumé :
The isolation and culture of dorsal root ganglion (DRG) neurons cause adaptive changes in the expression and regulation of ion channels, with consequences on neuronal excitability. Considering that not all neurons survive the isolation and that DRG neurons are heterogeneous, it is difficult to find the cellular subtype of interest. For this reason, researchers opt for DRG-derived immortal cell lines to investigate endogenous properties. The F-11 cell line is a hybridoma of embryonic rat DRG neurons fused with the mouse neuroblastoma line N18TG2. In the proliferative condition, F-11 cells do not display a gene expression profile correspondent with specific subclasses of sensory neurons, but the most significant differences when compared with DRGs are the reduction of voltage-gated sodium, potassium and calcium channels, and the small amounts of TRPV1 transcripts. To investigate if functional properties of mature F-11 cells showed more similarities with those of isolated DRG neurons, we differentiated them by serum deprivation. Potassium and sodium currents significantly increased with differentiation, and biophysical properties of tetrodotoxin (TTX)-sensitive currents were similar to those characterized in small DRG neurons. The analysis of the voltage-dependence of calcium currents demonstrated the lack of low threshold activated components. The exclusive expression of high threshold activated Ca2+ currents and of TTX-sensitive Na+ currents correlated with the generation of a regular tonic electrical activity, which was recorded in the majority of the cells (80%) and was closely related to the activity of afferent TTX-sensitive A fibers of the proximal urethra and the bladder. Responses to capsaicin and substance P were also recorded in ~20% and ~80% of cells, respectively. The percentage of cells responsive to acetylcholine was consistent with the percentage referred for rat DRG primary neurons and cell electrical activity was modified by activation of non-NMDA receptors as for embryonic DRG neurons. These properties and the algesic profile (responses to pH5 and sensitivity to both ATP and capsaicin), proposed in literature to define a sub-classification of acutely dissociated rat DRG neurons, suggest that differentiated F-11 cells express receptors and ion channels that are also present in sensory neurons.
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Powell, Geoffrey M. L. « Attaching cells to finite complexes, with an application to elliptic spaces ». Mathematical Proceedings of the Cambridge Philosophical Society 119, no 3 (avril 1996) : 483–91. http://dx.doi.org/10.1017/s0305004100074351.
Texte intégralRésumé :
Suppose that f; Sn → E is a continuous map from the n-sphere to a 1-connected CW complex E, with n ≥ 2. One may suppose that f is a cofibration, so that there is a cofibration sequence , with f the attaching map of the cell en+1. Consider the homotopy fibre F of the inclusion E ↪ B, so that there is a homotopy fibration let δ; ΩB → F be the connectant of this fibration. The following definition is given by Félix and Lemaire in [11]: Definition 1·1. Suppose that k is a field of characteristic p ≥ 0. The attaching map f:Sn → E is: 1. p-inert if is surjective; 2. p-lazy if is zero; where H˜ denotes reduced homology and coefficients are taken in the field k.
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Chen, Xu, Ying Qin, Xinru Song, He Li, Yue Yang, Jiazhuang Guo, Tingting Cui, Jiafei Yu, Cai-Feng Wang et Su Chen. « Green Synthesis of Carbon Dots and Their Integration into Nylon-11 Nanofibers for Enhanced Mechanical Strength and Biocompatibility ». Nanomaterials 12, no 19 (26 septembre 2022) : 3347. http://dx.doi.org/10.3390/nano12193347.
Texte intégralRésumé :
Carbon dots (CDs) have been extensively explored to show good optical features, low toxicity, and good biocompatibility. Herein, we report the new synthesis of forsythia-derived CDs (F-CDs) and their incorporation into Nylon-11 nanofibers for improved mechanical properties and biocompatibility. F-CDs are prepared from a Chinese herb forsythia via a magnetic hyperthermia method in 90 s without the use of any organic solvents. The as-prepared F-CDs with rich surface functional groups can be well embedded into Nylon-11 nanofibers via electrospinning, providing Nylon-11/F-CD nanofiber mats with remarkably enhanced mechanical properties. With the incorporation of F-CDs at 10 wt% into the Nylon-11 nanofiber mats, the tensile strength increases from 7.5 to 16.6 MPa, and the elongation ratio at break increases from 39% to 125%. Moreover, the Nylon-11/F-CD nanofiber mats exhibit excellent cytocompatibility towards L929 fibroblast cells with cell viability of 96%. These findings may guide the development of various CD-embedded nanofiber mats with good mechanical properties and biocompatibility potentially useful for biomedical applications, such as tissue engineering scaffolds or wound dressing.
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Zhang, Hongyu, Peter J. Wickley, Sayantani Sinha, Ian N. Bratz et Derek S. Damron. « Propofol Restores Transient Receptor Potential Vanilloid Receptor Subtype-1 Sensitivity via Activation of Transient Receptor Potential Ankyrin Receptor Subtype-1 in Sensory Neurons ». Anesthesiology 114, no 5 (1 mai 2011) : 1169–79. http://dx.doi.org/10.1097/aln.0b013e31820dee67.
Texte intégralRésumé :
Background Cross talk between peripheral nociceptors belonging to the transient receptor potential vanilloid receptor subtype-1 (TRPV1) and ankyrin subtype-1 (TRPA1) family has been demonstrated recently. Moreover, the intravenous anesthetic propofol has directly activates TRPA1 receptors and indirectly restores sensitivity of TRPV1 receptors in dorsal root ganglion (DRG) sensory neurons. Our objective was to determine the extent to which TRPA1 activation is involved in mediating the propofol-induced restoration of TRPV1 sensitivity. Methods Mouse DRG neurons were isolated by enzymatic dissociation and grown for 24 h. F-11 cells were transfected with complementary DNA for both TRPV1 and TRPA1 or TRPV1 only. The intracellular Ca concentration was measured in individual cells via fluorescence microscopy. After TRPV1 desensitization with capsaicin (100 nM), cells were treated with propofol (1, 5, and 10 μM) alone or with propofol in the presence of the TRPA1 antagonist, HC-030031 (0.5 μM), or the TRPA1 agonist, allyl isothiocyanate (AITC; 100 μM); capsaicin was then reapplied. Results In DRG neurons that contain both TRPV1 and TRPA1, propofol and AITC restored TRPV1 sensitivity. However, in DRG neurons containing only TRPV1 receptors, exposure to propofol or AITC after desensitization did not restore capsaicin-induced TRPV1 sensitivity. Similarly, in F-11 cells transfected with both TRPV1 and TRPA1, propofol and AITC restored TRPV1 sensitivity. However, in F-11 cells transfected with TRPV1 only, neither propofol nor AITC was capable of restoring TRPV1 sensitivity. Conclusions These data demonstrate that propofol restores TRPV1 sensitivity in primary DRG neurons and in cultured F-11 cells transfected with both the TRPV1 and TRPA1 receptors via a TRPA1-dependent process. Propofol's effects on sensory neurons may be clinically important and may contribute to peripheral sensitization to nociceptive stimuli in traumatized tissue.
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Khandros, Eugene, Peng Huang, Scott A. Peslak, Belinda Giardine, Zhe Zhang, Cheryl A. Keller, Ross C. Hardison et Gerd A. Blobel. « Understanding Heterogeneity of Fetal Hemoglobin Induction through Comparative Analysis of Stage-Matched F- and a-Cells ». Blood 134, Supplement_1 (13 novembre 2019) : 981. http://dx.doi.org/10.1182/blood-2019-124099.
Texte intégralRésumé :
Reversing the developmental switch from fetal (HbF, α2γ2) to adult (HbA, α2β2) hemoglobin is an important therapeutic approach in sickle cell disease (SCD) and β-thalassemia. Elevated HbF levels due to genetic variation or through therapeutic induction by hydroxyurea (HU) attenuate the severity of both disorders. HbF in healthy individuals, SCD patients, and patients treated with HU is present in a heterocellular fashion in a subset of red blood cells known as F-cells. Despite over 50 years of observations of F-cells, it is not known why only some cells in a genetically identical population are able to express HbF or respond to pharmacological inducers. Adult F-cells can potentially represent a reversion to a fetal-like epigenetic and transcriptional program, or alternatively isolated transcriptional or posttranscriptional events at the γ-globin genes. Here we set out to understand the heterogeneity of HbF activation and gain insights into whether the mechanisms underlying the heterocellular response are similar or distinct in response to different HbF inducers. To this end we developed techniques to purify differentiation stage-matched late erythroblast F-cells and non-F cells (A-cells) from the human HUDEP2 erythroid cell line and primary CD34 cell erythroid cultures using a reversible fixation protocol enabling extraction of high-quality RNA and protein. Purified F-cells from both sources were enriched for γ-globin transcripts by 200-500 fold by RT-PCR, validating the purification scheme. We profiled these cells by RNA-seq using a modified method that depletes globin mRNAs and ribosomal RNAs and is capable of detecting low abundance transcripts, as well as by mass spectrometry using size fractionation to increase the number of detected proteins. In differentiated clonal HUDEP2 cells, differences between F-cells and A-cells were remarkably small, with only 62 differentially expressed transcripts and 20 differentially expressed proteins. Top differentially expressed transcripts were γ-globin and the non-coding β-globin locus transcripts BGLT3 and HBBP1. Interestingly, there were no significant changes in known HbF regulators BCL11A, LRF, and HRI at the RNA or protein level. Gene set enrichment analysis (GSEA) using a previously generated set of differentially expressed transcripts from adult and fetal-derived CD34 erythroid cultures showed enrichment of fetal transcripts in F-cells and adult transcripts in A-cells. We also carried out transcriptome analysis of sorted matched late erythroblast F-cells and A-cells from human CD34+ cell erythroid cultures at different time points. Similar to HUDEP2 cells, only small numbers of transcripts were differentially expressed (33 at 8 days, 17 at 11 days, and 261 at 14 days). BCL11A, LRF, and HRI were not differentially expressed at the earlier timepoints, and BCL11A and HRI were at most decreased by about 20% at the 14-day mark. GSEA analysis did not show fetal transcript enrichment in day 8. At days 11 and 14, there was some enrichment of fetal transcripts in F-cells but not to the degree of HUDEP2 cells. Finally, we analyzed sorted F- and A-cells from day 11 CD34+ erythroid cultures treated with hydroxyurea and pomalidomide. Again, differences between F- and A-cells were small with hydroxyurea treatment (53 transcripts) and more significant with pomalidomide treatment (400 transcripts). We have successfully established an approach to analyze stage-matched γ-globin containing cells from a genetically identical starting population, with high degree of enrichment. Our preliminary data indicate that these cells are overall highly similar to non-γ-containing cells, but do show some enrichment of fetal-specific transcripts, more so in HUDEP2 cells. The differences between F- and A- cells are overall smaller than those observed by us and others in profiling of fetal and adult-derived erythroblasts. This suggests that F-cells are not formed by reversion to a fetal-like state but rather through specific changes at the β-globin locus. Importantly, we do not find differential levels of any known γ-globin regulators, suggesting an alternative mechanism for the heterocellular expression pattern. Studies are currently ongoing to carry out epigenetic profiling of F-cells. Disclosures Blobel: Bioverativ: Research Funding; Pfizer: Research Funding.
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Dover, GJ, et SH Boyer. « Fetal hemoglobin-containing cells have the same mean corpuscular hemoglobin as cells without fetal hemoglobin : a reciprocal relationship between gamma- and beta-globin gene expression in normal subjects and in those with high fetal hemoglobin production ». Blood 69, no 4 (1 avril 1987) : 1109–13. http://dx.doi.org/10.1182/blood.v69.4.1109.1109.
Texte intégralRésumé :
Abstract We have developed methodology that allows comparison of the mean corpuscular hemoglobin (MCH) of fetal hemoglobin (HbF)-containing red cells (F cells) with the MCH of non-F cells from the same individual. To do this, suspensions of peripheral blood erythrocytes and their internal contents are fixed with an imidodiester, dimethyl-3,3′- dithiobispropionimidate dihydrochloride (DTBP). Thereafter fixed cells are made permeable to antisera by treatment with Triton X-100 and isopropanol, reacted with a mouse monoclonal antibody (MoAb) against HbF, and then with fluorescein-conjugated antimouse IgG. No appreciable hemoglobin is lost during such manipulation. Red cells from a diversity of subjects were thus treated and examined microscopically, first by transmitted light and then by epifluorescence. A direct correlation between Coulter-derived MCH and mean absorbance of 415 nm transmitted light was found for 100 unfixed (r = 0.96) and for 100 antibody-treated fixed-permeabilized red cells (r = 0.99) among individuals selected so as to provide a range of Coulter MCH values between 20 and 35. Comparisons of microscopically derived MCH of F cells and non-F cells were statistically nondistinguishable (P greater than 0.05) in all subjects. Such comparisons included normal individuals (less than 1% F cells), SS patients (7% to 48% F cells), subjects with congenital anemia (22% to 65% F cells), individuals with heterocellular hereditary persistence of HbF (HPFH) (12% to 21% F cells), and heterozygotes for beta + thalassemia (11% to 31% F cells). We conclude that gamma- and beta-globin production within F cells is regulated in a reciprocal fashion both among normal individuals and among individuals with elevated HbF production.
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Dover, GJ, et SH Boyer. « Fetal hemoglobin-containing cells have the same mean corpuscular hemoglobin as cells without fetal hemoglobin : a reciprocal relationship between gamma- and beta-globin gene expression in normal subjects and in those with high fetal hemoglobin production ». Blood 69, no 4 (1 avril 1987) : 1109–13. http://dx.doi.org/10.1182/blood.v69.4.1109.bloodjournal6941109.
Texte intégralRésumé :
We have developed methodology that allows comparison of the mean corpuscular hemoglobin (MCH) of fetal hemoglobin (HbF)-containing red cells (F cells) with the MCH of non-F cells from the same individual. To do this, suspensions of peripheral blood erythrocytes and their internal contents are fixed with an imidodiester, dimethyl-3,3′- dithiobispropionimidate dihydrochloride (DTBP). Thereafter fixed cells are made permeable to antisera by treatment with Triton X-100 and isopropanol, reacted with a mouse monoclonal antibody (MoAb) against HbF, and then with fluorescein-conjugated antimouse IgG. No appreciable hemoglobin is lost during such manipulation. Red cells from a diversity of subjects were thus treated and examined microscopically, first by transmitted light and then by epifluorescence. A direct correlation between Coulter-derived MCH and mean absorbance of 415 nm transmitted light was found for 100 unfixed (r = 0.96) and for 100 antibody-treated fixed-permeabilized red cells (r = 0.99) among individuals selected so as to provide a range of Coulter MCH values between 20 and 35. Comparisons of microscopically derived MCH of F cells and non-F cells were statistically nondistinguishable (P greater than 0.05) in all subjects. Such comparisons included normal individuals (less than 1% F cells), SS patients (7% to 48% F cells), subjects with congenital anemia (22% to 65% F cells), individuals with heterocellular hereditary persistence of HbF (HPFH) (12% to 21% F cells), and heterozygotes for beta + thalassemia (11% to 31% F cells). We conclude that gamma- and beta-globin production within F cells is regulated in a reciprocal fashion both among normal individuals and among individuals with elevated HbF production.
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Skyberg, Jerod A., et Carolyn A. Lacey. « Hematopoietic MyD88 mediates protection against virulent Francisella tularensis infection via IFN-γ, but does not require myeloid or dendritic cell MyD88 signaling ». Journal of Immunology 198, no 1_Supplement (1 mai 2017) : 148.24. http://dx.doi.org/10.4049/jimmunol.198.supp.148.24.
Texte intégralRésumé :
Abstract Francisella tularensis is a highly infectious intracellular bacterium that causes the potentially fatal disease tularemia. MyD88 has previously been shown to confer protection against both attenuated and virulent strains of F. tularensis, however the underlying mechanisms of protection are largely unexplored. Here we investigated cell-specific mechanisms of protection against virulent F. tularensis infection using mice with conditional MyD88 deficiencies. MyD88 deficiency in myeloid or dendritic cells did not enhance susceptibility to F. tularensis, regardless of the route of infection. In addition, myeloid or dendritic cell MyD88 deficiency did not markedly hinder the production of inflammatory cytokines. In contrast, MyD88−/− mice, or mice with hematopoietic MyD88 deficiency display elevated bacterial burdens, and markedly reduced cytokine levels. While IL-12 levels were not diminished in MyD88−/− or hematopoietic MyD88-deficient mice infected with F. tularensis, IFN-γ production was abolished in these animals. In particular, IFN-γ production by splenic NK cells was abated in mice lacking hematopoietic MyD88. Neutralization of IFN-γ from wild-type, but not hematopoietic MyD88-deficient mice, resulted in elevated tissue F. tularensis burdens. Caspase-1/11−/− mice also displayed enhanced bacterial burdens, diminished serum IL-18 levels, and reduced IFN-γ production. Collectively, our data shows that hematopoietic MyD88 is required for IFN-γ production that is protective against F. tularensis infection. As IL-18 requires MyD88 for signaling to induce IFN-γ production, MyD88 signaling in hematopoietic cells, such as NK cells, may result in the production of protective IFN-γ via caspase1/11 dependent IL-18.
Thèses sur le sujet "F-11 cells"
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MONZA, LAURA. « In vitro models for studying oxaliplatin neurotoxic effects ». Doctoral thesis, Università degli Studi di Milano-Bicocca, 2019. http://hdl.handle.net/10281/241333.
Texte intégralRésumé :
L’oxaliplatino (OHP) è un composto del platino di terza generazione, usato in combinazione con 5-fluorouracile e leucovorin per il trattamento del tumore al colon-retto metastatico. L’assunzione di OHP è in grado di indurre l’insorgenza di due fenomeni di tossicità, acuto e cronico. La tossicità acuta è caratterizzata da disestesie e parestesie transitorie esacerbate dal contatto col freddo. La gravità di questi sintomi è predittiva per lo sviluppo della neuropatia sensoriale cronica.
Sebbene i meccanismi patogenetici alla base della tossicità acuta non siano stati del tutto chiariti, l’ipotesi più accreditata supporta un maggiore coinvolgimento dei canali del sodio voltaggio-dipendenti. Tuttavia, gli studi sino ad ora condotti si sono focalizzati solo su aspetti specifici della risposta elettrofisiologica cellulare ed hanno utilizzato concentrazioni di OHP molto elevate producendo risultati a volte controversi. Per questi motivi, il nostro obiettivo è stato quello di valutare gli effetti di una concentrazione di OHP più fisiologica sulle proprietà elettriche di diversi modelli di neuroni sensoriali. Poiché i neuroni dei gangli della radice dorsale (DRG) rappresentano il principale bersaglio farmacologico dei composti del platino, i nostri studi sono stati condotti incubando con OHP (7.5 μM) cellule F-11 differenziate. Abbiamo quindi studiato le possibili alterazioni prodotte dal farmaco sul potenziale di riposo (Vrest), sulle caratteristiche del potenziale d’azione (AP) e sulle proprietà biofisiche dei canali voltaggio-dipendenti di sodio e potassio. Le loro proprietà elettrofisiologiche sono state studiate con la tecnica del patch-clamp in configurazione whole-cell. Il cisplatino (CDDP 15 μM) è stato usato come composto di controllo per verificare l'esclusività degli effetti indotti da OHP.
Inoltre, al fine di convalidare i risultati raccolti sulle cellule F-11 differenziate, i nostri esperimenti sono stati riprodotti su culture primarie di neuroni sensoriali derivati da DRG di embrioni di ratto o di ratti adulti.
Rispetto alle cellule non trattate, l’incubazione con OHP ha determinato una depolarizzazione del Vrest, una riduzione della frequenza di scarica di AP, un aumento della densità di corrente di sodio e una riduzione della densità di corrente dei canali del potassio ERG. Tuttavia, la somministrazione di OHP non ha avuto alcuna influenza sui canali del potassio delayed-rectifier e sulla durata di AP indotti. Inoltre, OHP ha determinato uno spostamento delle curve di attivazione e di inattivazione delle correnti di sodio TTX-sensibili verso potenziali più negativi ed un aumento della risultante corrente finestra. Un comportamento simile è stato osservato anche per i canali ERG. Al contrario, il trattamento con CDDP non ha determinato variazioni del Vrest ed ha causato una riduzione della frequenza di scarica, un aumentato della durata di PA ed una riduzione della densità di corrente di potassio delayed-rectifier, di ERG e di sodio.
Nelle colture primarie di neuroni sensoriali embrionali, l'incubazione con OHP ha indotto un aumento della frazione di neuroni in grado di generare PA multipli evocati ed un aumento delle densità di corrente di sodio e di potassio.
Infine, i dati raccolti dalle colture primarie di neuroni derivati da ratti adulti hanno mostrato che la somministrazione di OHP per 24 ore aumenta la densità di corrente di sodio, mentre non ha alcun effetto sugli altri parametri studiati.
In conclusione, i nostri risultati hanno messo in luce diversi target di OHP a livello dei neuroni sensoriali, agendo sia sui canali del sodio che del potassio. Inoltre i dati raccolti, suggeriscono che le cellule F-11 differenziate rappresentano un buon modello cellulare per lo sviluppo di strategie farmacologiche volte a prevenire l'insorgenza di neurotossicità causata da un’alterazione della funzionalità dei canali del sodio.Chemotherapy-induced peripheral neurotoxicity is one of the most common and often dose limiting side effects of anticancer drugs. Among others, oxaliplatin (OHP) is a third generation platinum compound used in combination with 5-fluorouracil and leucovorin as an efficient treatment for metastatic colorectal cancer. Unlike other compounds of the same class, oxaliplatin may also cause an acute syndrome characterized by transient cold-induced dysesthesias and paresthesias located at limb extremities and at perioral area. The severity of these symptoms is predictive of the development of chronic and cumulative sensory neuropathy. Hence, unraveling the mechanisms underlying the acute syndrome should not be considered a secondary aim. Since Adelsberger et al. (Eur J Pharmacol 406:25-32, 2000) first described the effects of OHP on voltage-dependent sodium channels, many in vitro studies on different animal models supported the hypothesis of a major involvement of these channels in the acute syndrome. However, all of these works used very high OHP concentrations and focused on single aspects of the overall electrophysiological cellular response to OHP administration and gave controversial results. For these reasons, our aim was to study the effects of an OHP concentration comparable to the one estimated in patients’ blood on the electrical properties of different models of sensory neurons. We thus investigated the possible alterations produced by the drug on membrane resting potential (Vrest), on the main action potential (AP) features and on the biophysical properties of voltage-dependent sodium and potassium channels. Since dorsal root ganglion (DRG) neurons represent the main pharmacological target of platinum compounds, we incubated differentiated F-11 cells (rat DRG neurons x mouse neuroblastoma N18TG-2 cell line) for 24 or 48 h with 7.5 µM OHP. Their electrophysiological properties were investigated by the patch-clamp technique in the whole-cell configuration. Cisplatin (CDDP 15 µM) was used as reference compound to verify the exclusivity of OHP-induced effects. Finally, in order to validate the results collected with the differentiated F-11 cells, our experiments were reproduced on primary sensory neurons deriving from the dissociation of isolated embryonic and adult rat DRGs. Compared to untreated cells, treated F-11 cells displayed depolarized Vrest, decreased firing frequency, increased sodium current density and reduced ERG (ether-à-go-go-related gene) potassium current density. However, OHP administration did not affect the delayed rectifying potassium channels and the duration of induced APs. In TTX-sensitive sodium currents, OHP shifted both steady-state activation and inactivation curves towards more negative potentials and caused an expansion of the window current. A similar shift of both activation and inactivation curves was observed for ERG channels. In contrast, CDDP caused no effect on Vrest, decreased firing frequency, increased AP duration, and reduced sodium, ERG and delayed rectifier potassium current densities. In embryonic primary DRG neuron cultures, OHP incubation induced a significant increase of the fraction of sensory neurons able to generate multiple evoked APs and of voltage-dependent sodium and potassium current densities. Vrest and the firing frequency were not affected by the treatment. Lastly, data collected on primary DRG neuron cultures derived from adult rats showed that administration of OHP for 24h significantly increased sodium current density while no effects were produced on the other parameters of interest. In conclusion, the collected data indicate that OHP has different targets on DRG neurons, acting on both sodium and potassium channels, and suggest that differentiated F-11 cells represent a good cellular model for the development of pharmacological strategies aimed at preventing the onset of neurotoxicity caused by sodium channel dysfunction.
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Zielecki, Julia [Verfasser], Thomas F. [Akademischer Betreuer] Meyer, Richard [Akademischer Betreuer] Lucius et Stefan [Akademischer Betreuer] Bereswill. « Establishment of in vitro-infection models for Chlamydia trachomatis based on human primary cells and primary tissue / Julia Zielecki. Gutachter : Thomas F. Meyer ; Richard Lucius ; Stefan Bereswill ». Berlin : Humboldt Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2011. http://nbn-resolving.de/urn:nbn:de:kobv:11-100196653.
Texte intégralLivres sur le sujet "F-11 cells"
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Hoshiko, Masaki. F*cking Hard Sudoku Puzzle Book #11 : The 300 Worst Sudoku Puzzles in History That Will Destroy Your Life and Brain Cells Just at the First Puzzle. Independently Published, 2019.
Trouver le texte intégralChapitres de livres sur le sujet "F-11 cells"
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S.N. Chaitanya, Nyshadham, et Sibani Sahu. « Mineral Deficiencies a Root Cause for Reduced Longevity in Mammals ». Dans Mineral Deficiencies - Genes, Diet and Disease Interface [Working Title]. IntechOpen, 2020. http://dx.doi.org/10.5772/intechopen.94276.
Texte intégralRésumé :
Metals, inorganic compounds and their elements that act as cofactors for enzymes that play an essential role in various biological processes constitute mineral nutrients. Their primary source is soil and enters the climax consumers in food chain through plants as they contain most minerals that are essential for humans. They are required in small and precise amounts according to their requirement they were classified as Major (phosphorous (P), potassium (K)), Secondary (calcium (Ca), magnesium (Mg), sulphur (S)), Minor/trace/rare (Boron (B), chlorine (Cl), chromium (Cr), fluoride(F), iodine (I), iron (Fe), manganese (Mn), molybdenum (Mo), nickel (Ni), selenium (Se), sodium (Na), vanadium (V) and zinc (Zn)). The daily requirement of minerals for individuals for effective biological function inside the cell is known as recommended dietary allowance (RDA) that varies for element. The daily requirement of major element is up to 10 g/d, whereas secondary and micro minerals was 400 - 1500 mg/d and 45 μg/d - 11 mg/d, respectively. Meats, vegetables, fruits, grains contains high amount of minerals that protect humans from mineral deficiencies. Some of the mineral deficiencies include ageing, cancer, hair loss etc. The key for these root problems include supplementation of healthy foods rich in minerals and understanding the importance of food by nutrition education, practice of physical activity, and about food habits. A detailed understanding of each mineral and their biological importance through mechanism of action studied in detail to overcome their deficiencies.
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Taber, Douglass F. « Flow Chemistry ». Dans Organic Synthesis. Oxford University Press, 2017. http://dx.doi.org/10.1093/oso/9780190646165.003.0018.
Texte intégralRésumé :
Arturo Macchi of the University of Ottawa and Dominique M. Roberge of Lonza summarized (Org. Process Res. Dev. 2014, 18, 1286) a “toolbox approach” for the evolution from batch to continuous chemical synthesis. Michael D. Organ of York University developed (Org. Process Res. Dev. 2014, 18, 1315) a flow reactor with inline analytics, and Timothy D. White of Eli Lilly described (Org. Process Res. Dev. 2014, 18, 1482) the continuous production of solid products under flow conditions. Electrochemical reduction and oxidation are particularly easy under flow conditions. Steven V. Ley of the University of Cambridge oxidized (Org. Lett. 2014, 16, 4618) 1 under flow conditions, then condensed the product with tryptamine 2 to prepare the indole alkaloid Nazlinine 3. Thomas Wirth of Cardiff University electrolyzed (Org. Process Res. Dev. 2014, 18, 1377) the carbonate 4 in a non-divided cell to return the deprotected phenol 5. Timothy Noël of the Eindhoven University of Technology gathered (Chem. Eur. J. 2014, 20, 10562) an overview of photochemical transformations under flow conditions. Kevin I. Booker-Milburn of the University of Bristol observed (Chem. Eur. J. 2014, 20, 15226) superior yields for the coupling of 6 with 7 to form 8 under flow compared to batch conditions. Koichi Fukase of Osaka University and Ilhyong Ryu of Osaka Prefecture University converted (Chem. Eur. J. 2014, 20, 12750) 9 selectively to 10 under flow conditions. Alexei A. Lapkin, also of the University of Cambridge, optimized (Org. Process Res. Dev. 2014, 18, 1443) the singlet oxygen conversion of 11 to 12. Shawn K. Collins of the Université de Montréal cyclized (Org. Process Res. Dev. 2014, 18, 1571) 13 to 14. There have been several advances in the use of enzymes under flow conditions. Rodrigo O. M. A. de Souza of the Federal University of Rio de Janeiro found (Org. Process Res. Dev. 2014, 18, 1372) that lipase in a microemulsion-based organogel efficiently converted coupled 15 with 16 to make 17. Timothy F. Jamison of MIT developed (Org. Lett. 2014, 16, 6092) a catch-and-release protocol for the reductive amination of 18 with 19 to give 20.
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« oPnosDsA ib F le m lo o c le a c ti uolnesdoefdCurcoemdefrrosm ys t M em AI EaAntt ig e e st nss Understanding of the biochemical structures and molecular basis of Rh antigens is emerging rapidly. Absence of Rh antigens, as occurs in the RhnuN phenotype, compromises the integrity of red cells and cells from people with an RhnuN phenotype have been extensively studied. These studies contributed to the recognition of Rh polypeptides and some related glycoproteins [see 20,21,22]. Partial amino acid sequencing of the proteins in Bristol, Paris and Baltimore [23,24,25] led to recognition of involvement of two genes and isolation of cDNA by the Paris and Bristol workers [26,27] and cloning of the D gene [28]. One gene is responsible for the D polypeptide and another for the C and E series of antigens. However, although encoded by the same gene there is evidence that the C and E series of antigens are carried by different proteins. The molecular genetic basis of Rh antigens is discussed in another presentation. Immune precipitation using anti-D, -c, -E or R6A antibodies demonstrated the proteins which carried the Rh antigens. Two bands are co-precipitated by anti-D : one with an apparent Mr 30,000 called D30 polypeptide by the Bristol group and the other a diffuse band of 50-100 kD called the D50 polypeptide. Similar bands were observed when immune precipitation were done using anti-c, -E or R6A [see 20-22]. The D30 polypeptide was an unusual membrane protein because it was not glycosylated, the gene producing this protein and the other Rh protein were subsequently cloned. Assignment of the genes to chromosome 1p34-p36 confirmed that they are responsible for the Rh polymorphism [see 22]. The role of the Rh glycoproteins, the diffuse band of 50-1 OOkD, is not yet understood : the gene encoding the Rh glycoprotein when cloned was assigned to chromosome 6p21-qter [29]. » Dans Transfusion Immunology and Medicine, 192. CRC Press, 1995. http://dx.doi.org/10.1201/9781482273441-11.
Texte intégralActes de conférences sur le sujet "F-11 cells"
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Montani, N., S. B. Solerte, G. Gamba, M. Fioravanti et E. Ferrari. « RELATIONSHIPS BETWEEN HAEMOSTATIC ENDOTHELIAL FUNCTIONS AND GLOMERULAR FILTRATION RATE IN SHORT-TERM TYPE I DIABETES MELLITUS ». Dans XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643101.
Texte intégralRésumé :
It is known that the increase of glomerular filtration rate (GFR) represents an early sign of diabetic nephropathy. The changes of endothelial functions observed in diabetes might play a role in this respect. As F VIII vWF and fibronectin are synthetized by endothelial cells, we evaluated these components in 33 diabetic patients with short-term Type I (insulin dependent) diabetes mellitus, without retinopathy and macro-vascular complications. 15 pts. (mean age 29 ± 7 yrs; mean diabetes duration 2.9 ± 0.9 yrs) presented high GFR (154 ± 19 ml/min per 1.73 m2 ; albuminuria 7.2 ± 3.2 μg/min) and 18 pts. (mean age 30 ± 6 yrs; mean diabetes duration 3.0 ± 1 yrs) normal GFR (105 ± 11 ml/min per 1.73 m2 ; albuminuria 5 ± 2.8 μg/min).The following results were obtained:In conclusion the significant increase of FVIIIR:Ag and fibronectin levels in short-time type I diabetic patients with high GFR suggests an early endothelial cell function damage also related to the Door metabolic control.
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Ma, Hsiao-Kang, Jyun-Sheng Wang, Shih-Han Huang, Yu-Jen Huang et Yao-Zong Kuo. « Numerical Study of Different Anode and Cathode Channel Design on the Performance of Piezoelectric Proton Exchange Membrane Fuel Cells (PZT-PEMFCs) ». Dans ASME 2009 7th International Conference on Fuel Cell Science, Engineering and Technology. ASMEDC, 2009. http://dx.doi.org/10.1115/fuelcell2009-85086.
Texte intégralRésumé :
Previous studies [18, 19] have indicated that a novel ribbed PZT-PEMFC design has been developed, and that a three-dimensional, transitional model has been successfully built to study its major characteristics and fuel cell performance. A ribbed cathode channel can reduce internal resistance and double current density. At a higher PZT vibration frequency (f = 64 Hz), an air-breathing PZT-PEMFCs device compresses more oxygen into the catalyst layer and thus enhances the electrochemical reaction, resulting in a higher current output. On the other hand, the accumulated water vapor may be pumped out from the cathode channel during the compression process. Previous studies [11, 12] also demonstrated that serpentine and interdigitated flow fields could induce better performance than other flow fields in traditional PEMFCs, such as parallel and pin-type. In this study, the 3-D theoretical model of PZT-PEMFCs has been successfully developed in order to investigate the effects of anode and cathode channel designs on the performance of PZT-PEMFCs. Different cathode open area ratios, which are 80.5%, 63.2%, 47.9%, and 34.7%, were chosen for consideration of current density, PZT vibration frequency, and species concentrations. The results show that the cathode open area ratio of 47.9% is a better choice than 80.5%, 63.2%, or 34.7%. The results also establish that a lower vibration frequency may draw less air into the cathode channel, cause water vapor accumulation in the space of the electrochemical reaction area, and ultimately cause a drop in current over time. On the other hand, the designs of the anode flow field are found to have a big influence on the current density and water vapor profiles. The simulation results prove that the interdigitated flow field in the anode side, which is different from the traditional PEMFCs, performs much better than the serpentine and parallel flow fields.
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Nagy, J. A., et H. F. Devorak. « FIBRINOGEN INFLUX AND FIBRIN ACCUMULATION IN ASCITES TUMORS ». Dans XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643668.
Texte intégralRésumé :
Fibrin gel matrix organizes solid tumors into discrete cell nests and providesa provisional matrix for mature stroma generation. Fibrin deposits result fromlocal extravasation of plasma fibrinogen followed by extravascular coagulationand crosslinking. Unlike solid tumors, ascites tumor cells in body cavities grow in suspension, and are not envelopedin fibrin gel. The lack of fibrin gel in ascites tumors has several possible explanations: 1. Peritoneal wall blood vessels are impermeable to molecules as large as fibrinogen. 2. Fibrinogen leaks from peritoneal vessels, but is clotted to fibrin and never reaches the peritoneal cavity. 3. Fibrinogen leaks from vessels, reaches the peritoneal cavity, and is rapidly degraded. To distinguish among these possibilities we investigated the permselective properties of the peritoneal wall. We measured the influx of intravenously (iv) administered, fluorescein-labeled dextrans (FITC-D) of varying size into the peritonea of normal and tumor or inflammatory ascites-bearing mice and guinea pigs. FITC-D of MW 70-5,000 kD leaked from vessels and ∽ 10-fold more entered MOT or TA3/St tumor-associated or serotonin-induced inflammatory ascites than normal peritonea. In tumor bearing animals, 3-20% of injected FITC-D entered the peritoneum within 15-30 min., all of it intact by gel exclusion chromatography. We also investigated the influx of fluorescein-labeled fibrinogen (FITC-F). At 30-60 min after iv injection, FITC-F hadextravasated into the peritoneal wall as judged by fluorescence microscopy (tumor bearing ≫ normal animals). Moreover, monoclonal antibodies reactivewith fibrin, but not fibrinogen, stained such deposits by immunohistochemistry in guinea pigs bearing line 1 or line 10 ascites tumors. Following iv injection of 125l-fibrinogen, as much as 11% of injected radioactivity accumulated in tumor ascites fluid over1-3 hrs (versus ∽1% in normal controls). In both, only ∽half was clottableand 3040% was of low MW (≤.10 kD). In contrast, iv 125I-albumin also entered the peritoneal cavity at an enhanced rate but showed no evidence of degradation. We conclude that fibrinogen extravasates from peritoneal vessels,thatleakage is increased in ascites tumor-bearing animals, and that fibrinogen clots, at least in part, before it enters the peritoneal cavity. Also, fibrinogen undergoes selective degradation and much of the fibrinogen that does enter the peritoneum represents degradation products of fibrinogen/fibrin. The resulting lack of peritoneal fibrin gel matrixfavors the suspension pattern of ascites, as compared with solid tumor growth.
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Grabowski, F. E. « RHEOLOGY AND PRIMARY HEMOSTASIS ». Dans XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643986.
Texte intégralRésumé :
Overview The adhesion-aggregation of platelets to a site of vessel wall injury is a quintessential blood flow phenomenon. Firstly, platelets are driven to the vicinity of the vessel wall by a form of convective diffusion in which red cells both mechanically augment the effective platelet diffusivity (Turitto et al., Ind. Eng. Chem. Fund. 11:216-223, 1972; Grabowski et al., Ind. Eng. Chem. Fund. 11:224-232, 1972) and enhance the near-wall piatelet concentration (Ti11es and Eckstein, Microvasc Res., In press, 1987). Secondly, red cells subjected to physiologic shear forces are capable of secreting sufficient adenine nucleotides to induce primary platelet aggregation without themselves undergoing frank lysis (Reimers et al, Blood 64:1200-1206, 1984). This "humoral" effect of erythrocytes is likely to contribute to primary hemostasis in a shear stress-dependent manner. Thirdly, endothelial cells are able to modulate platelet aggregation at a site of vessel injury by producing prostacyclin (and perhaps other antithrombotic substances) in a manner which increases with vessel shear rate (Grabowski et al, Blood 62:301a, 1983); production for a large range of arterial shear rates appears to be limited by plasma-borne substrate (arachidonate). This manner of production ensures a concentration of prostacyclin in the near-wall region which remains relatively independent of shear rate.Imaging primary hemostasis. In our work, epi-fluorescence videomicroscopy has allowed real time imaging of platelet adhesion-aggregation to a simulated vessel wall injury. The injury model is an endothelial cell monolayer (ECM) across which, prior to ECM exposure to flowing blood, a 6-0 sterile suture is drawn in a direction transverse to flow. Microinjuries result which measure 70 ± 15μm (Mean ± SD) in width. The fluorescent label is the TAB murine monoclonal antibody (courtesy of Dr. R.P. McEver) directed against human platelet GPIIB, together with a fluorescein-conjugated goat F(ab')2 against murine inmunoglobulin. The injured ECM's, grown to confluence on rectangular cover glasses precoated with microfibrillar collagen, comprise one wall of a flow chamber mounted on a vertical microscope stage. On microinjury sites and at shear rates of 100 to 700 sec-1, computer-enhanced video images show adherence, remodelling and growth of chains of platelet aggregates. Aligned with the flow direction, these chains have a spacing of approximately 30)im, a length similar to the average endothelial cell diameter. One may speculate that such chains provide a scaffold for wound healing insofar as they are likely rich in agents chemotactic for leukocytes and in platelet-derived growth factor.Modulatory role of endothelium. When the ECM's are pre treated with 1.0 mM FC lysine acetyl sal icy late (LA), aggregate length increases (P<0.001) up totwo-fold, outflow levels by RIA of serum thromboxane B2 increase (8 of 8 paired runs), and outflow levels of prostacyclin by RIA for 6-Keto PGFiot decrease (5 of 7 paired runs). The Table gives data for one of four similar experiments at 270 sec-1 and following five minutes of flow. These data imply that products of ECM which are inhibitable by aspirin modulate local adhesion-aggregation; their inhibition, as by vasculitis or drugs, may give rise to thrombotic states.Bleeding disorders. Aggregate length is reduced in von Willebrand's disease (4 patients), Hermansky-Pudlak syndrome (2 patients), and after 300 mg oral aspirin (Tablet 4 donors). The reduction in the first two, however, is greater (P<0.01) than that for oral aspirin. With oral aspirin, further, there is a paradoxic increase in the percent platelet coverage of the injury area. Summary. Rheology has profound effects on the rate, structure, and modulation of primary hemostasis. Many of these effects can be studied via real-time, epi-fluorescence videomicroscopy of platelet adhesion-aggregation to a site of injury to an endothelial cell monolayer exposed to flowing blood. The model described has application to the study of thrombotic and hemostatic disorders and unstable angina.
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Siddiqua, Ayesha. « Salinity Effects on Symbiodinium sp. growth rate in Controlled conditions and produced Biomass Biochemical Characterization ». Dans Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2021. http://dx.doi.org/10.29117/quarfe.2021.0072.
Texte intégralRésumé :
Salinity is an abiotic influencer to the growth and the efficiency of the algal Symbiodinium that coexists in symbiosis with corals. In light of the high salinity conditions that prevail in the Arabian Gulf including the waters of Qatar, we observed the effect of salinity above local-ambient levels on Symbiodinium’s growth rate, biomass and its photosynthetic efficiency. Symbiodinium sp. extracted from Platygyra daedalea was launched in f/2 media in controlled incubator conditions at salinities of 30, 40 (control), 45, 50, 60 and 70 psu for 11 days. Subsamples were obtained and fixed for cell density counts and growth rate calculations. Photosynthetic efficiency was determined using an Aquapen, and biomass at the end of the experiment was sent for biochemical characterization. A two-way ANOVA test was performed on the data using SigmaPlot software. Our results indicated that at salinities 55 psu and greater, significant decline in both cell density and photosynthetic efficiency was observed. At 70 psu, growth rate was exclusively negatively affected, and biochemical compositions varied at all salinity levels with a notable increase in lipid content at 70 psu. Impact of high salinity has not been widely studied in the Arabian Gulf. Thus, this study will aid conservational efforts while also encouraging further studies on the contribution of abiotic factors to Symbiodinium sp. growth in the region.
Rapports d'organisations sur le sujet "F-11 cells"
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Idakwo, Gabriel, Sundar Thangapandian, Joseph Luttrell, Zhaoxian Zhou, Chaoyang Zhang et Ping Gong. Deep learning-based structure-activity relationship modeling for multi-category toxicity classification : a case study of 10K Tox21 chemicals with high-throughput cell-based androgen receptor bioassay data. Engineer Research and Development Center (U.S.), juillet 2021. http://dx.doi.org/10.21079/11681/41302.
Texte intégralRésumé :
Deep learning (DL) has attracted the attention of computational toxicologists as it offers a potentially greater power for in silico predictive toxicology than existing shallow learning algorithms. However, contradicting reports have been documented. To further explore the advantages of DL over shallow learning, we conducted this case study using two cell-based androgen receptor (AR) activity datasets with 10K chemicals generated from the Tox21 program. A nested double-loop cross-validation approach was adopted along with a stratified sampling strategy for partitioning chemicals of multiple AR activity classes (i.e., agonist, antagonist, inactive, and inconclusive) at the same distribution rates amongst the training, validation and test subsets. Deep neural networks (DNN) and random forest (RF), representing deep and shallow learning algorithms, respectively, were chosen to carry out structure-activity relationship-based chemical toxicity prediction. Results suggest that DNN significantly outperformed RF (p < 0.001, ANOVA) by 22–27% for four metrics (precision, recall, F-measure, and AUPRC) and by 11% for another (AUROC). Further in-depth analyses of chemical scaffolding shed insights on structural alerts for AR agonists/antagonists and inactive/inconclusive compounds, which may aid in future drug discovery and improvement of toxicity prediction modeling.