Thèses sur le sujet « Escherichia coli Genetics »
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Baldwin, Thomas John. « Disease mechanism of enteropathogenic Escherichia coli ». Thesis, University of Leicester, 1990. http://hdl.handle.net/2381/34445.
Texte intégralLim, C. K. « Cloning of Streptomyces genes in Escherichia coli ». Thesis, University of Leeds, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373850.
Texte intégralChua, K.-L. « Plasmid recombination in Escherichia coli K-12 ». Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383777.
Texte intégralMorgan, Brian Alexander. « The regulation of rpoBC in Escherichia coli ». Thesis, University of Edinburgh, 1986. http://hdl.handle.net/1842/15431.
Texte intégralAgostinho, Juliana Maria Avanci [UNESP]. « Diversidade genética, fatores de virulência e perfis de susceptibilidade a antimicrobianos de isolados de Escherichia coli provenientes do útero, da boca e das fezes de cadelas com piometra ». Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/108413.
Texte intégralA piometra canina é uma enfermidade caracterizada pela inflamação do útero com acúmulo de exsudatos, acometendo principalmente fêmeas adultas. Ocorre na fase lútea do ciclo estral em decorrência de alterações hormonais e infecção bacteriana. É reconhecida como uma das principais causas de morte em cadelas e a Escherichia coli é o principal patógeno associado a esta doença. O objetivo deste estudo foi isolar e identificar cepas de E. coli provenientes de conteúdo intra uterino, boca e fezes de cadelas diagnosticadas com piometra, estudar a prevalência de genes codificadores de fatores de virulência uropatogênicos das cepas obtidas testando ainda a semelhança genética entre as cepas isoladas de conteúdo intra uterino e boca e avaliar a susceptibilidade “in vitro” das bactérias isoladas frente a 12 agentes antimicrobianos. Setenta cepas de E. coli, 25 provenientes do conteúdo intra uterino, 26 provenientes da boca e 19 provenientes das fezes, isoladas de 6 cadelas foram examinadas por PCR. Entre as cepas examinadas, 67 (95,7%) foram positivas para o gene fim, 19 (27,1%) foram positivas para iss, 18 (25,7%) foram positivas para hly , 13 (18,5%) foram positivas para iuc e 12 (17,1%) foram positivas para usp. Três animais apresentaram grande similaridade entre as cepas de E. coli isoladas do conteúdo intra uterino e da boca, através da análise da diversidade genética realizada mediante emprego da técnica REP-PCR, ERIC-PCR e BOX-PCR. Resistência antimicrobiana predominante foi detectada para cefalotina (67,1%), ampicilina (65,7%), tetraciclina (61,4%) e nitrofurantoina (58,5%) entre as cepas isoladas. Resistência a múltiplos antimicrobianos foi detectada em 12 (48,0%) dos isolados do conteúdo intra uterino, em 22 (84,6%) dos isolados da boca e em 14 (73,6%) dos isolados das fezes...
Canine pyometra is a disease characterized by the inflammation of the uterus with accumulation of purulent discharge, affecting mainly adult animals. Occurs in the luteus phase of the estrus cycle in result of hormone alterations and generally is associated with bacterial infections. It is recognized as one of the main causes of disease and death in the bitch and Escherichia coli is the major pathogen associated with this disease. The aim of this study was to research, isolation and identification of Escherichia coli strains from intrauterine contents, mouth and feces of bitches diagnosed with pyometra, studying the prevalence of uropathogenic virulence factors genes in strains isolated, still testing the genetic similarity among strains isolated from intrauterine contents and mouth and evaluate the susceptibility in vitro of the isolated bacteria to 12 antimicrobial drugs. Seventy E. coli strains, 25 from intrauterine contents, 26 from mouth and 19 from feces isolated from six bitches were examined by PCR. Among the strains 67 (95.7%) were positive for fim, 19 (27.1%) were positive for iss, 18 (25.7%) were positive for hly, 13 (18.5%) were positive for iuc and 12 (17.1%) were positive for usp. Multiple antimicrobial resistance was detected for cephalothin (68.0%), nalidixic acid (56.0%) and ampicillin (56.0%) among the pyomera E. coli isolates. Multidrug resistance was found in 12 (48.0%) of the isolates from pyometra, in 22 (84.6%) of the isolates from mouth and in 14 (73.6%) of the isolates from feces...
Sendy, Bandar. « Studies on transcription in Escherichia coli ». Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7576/.
Texte intégralEnnis, Don Gregory. « Genetics of SOS mutagenesis ». Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184602.
Texte intégralWoodgate, R. « A mechanism for UV mutagenesis in Escherichia coli ». Thesis, University of Sussex, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375858.
Texte intégralHallewell, Jennyka, et University of Lethbridge Faculty of Arts and Science. « Shiga toxin-producing bacteriophage in Escherichia coli O157:H7 ». Thesis, Lethbridge, Alta. : University of Lethbridge, Deptartment of Biochemistry, 2008, 2008. http://hdl.handle.net/10133/776.
Texte intégralxv, 162 leaves : ill. ; 29 cm.
Miao, Yuanying, et 缪元颖. « The localization of E. coli persistent gene products ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45554791.
Texte intégralHo, Joanne Ming-Li. « Towards Sense Codon Reassignment in Escherichia Coli ». Thesis, Harvard University, 2015. http://nrs.harvard.edu/urn-3:HUL.InstRepos:26718706.
Texte intégralMedical Sciences
Picksley, S. M. « Inducible recombination and DNA repair in Escherichia coli K12 ». Thesis, University of Nottingham, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.332729.
Texte intégralTaylor, L. « The recB and recC gene products of Escherichia coli ». Thesis, University of Newcastle Upon Tyne, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355080.
Texte intégralDave, Emma. « The physiology of the Escherichia coli pyruvate dehydrogenase complex ». Thesis, University of Sheffield, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364242.
Texte intégralDoyle, Noel James. « Plasmid mediated error prone DNA repair in Escherichia coli ». Thesis, University of Liverpool, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.262031.
Texte intégralPickett, M. A. « Regulatory aspects of the gua operon of Escherichia coli ». Thesis, University of Southampton, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381272.
Texte intégralMalloch, Richard Anthony. « Ribonuclease III processing of Escherichia coli rpoBC messenger RNA ». Thesis, University of Edinburgh, 1990. http://hdl.handle.net/1842/15259.
Texte intégralStumpf, Jeffrey D. « Polyphosphate kinase regulates DNA polymerase IV in Escherichia coli ». [Bloomington, Ind.] : Indiana University, 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3253637.
Texte intégralTitle from PDF t.p. (viewed Nov. 19, 2008). Source: Dissertation Abstracts International, Volume: 68-02, Section: B, page: 0748. Adviser: Patricia L. Foster.
Cowan, Peter J. « Controlled production of tryptophan by genetically-manipulated strains of Escherichia coli ». Connect to thesis, 1992. http://repository.unimelb.edu.au/10187/1020.
Texte intégralDuring prolonged cultivation in the presence of antibiotic, the recombinant strain generated faster-growing, production-defective variants, which harboure mutated derivatives of pMU78. Insertion sequences were responsible for the two predominant types of mutation. The plasmid element ISI02 mediated deletions extending into the promoter-proximal region of the plasmid-borne trp operon. ISI0-Right, a chromosomal element, inserted into the promoter/trpE region of the plasmid. Three methods were employed to increase the structural stability of JP4153(pMU78) during the course of the production process. First, the growth of seed cultures was carried out at 30 degrees C, the permissive temperature for the trpS378 mutation carried by the host strain. Second, the seed culture medium was modified by the addition of yeast extract, which appeared to reduce the selective disadvantage conferred by the plasmid. Third, ISI02was deleted from pMU78 to create pMU88. (For complete abstract open document)
Park, Simon Fearon. « The biochemistry and genetics of osmoregulation in Escherichia coli ». Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.254420.
Texte intégralXiao, Minfeng, et 肖敏鳳. « The regulatory mechanisms and physiological functions of an outer membrane protein opmpW during anaerobic adaptation in Escherichia coli ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/206531.
Texte intégralpublished_or_final_version
Biological Sciences
Doctoral
Doctor of Philosophy
Huen, Shing-yan Michael, et 禤承恩. « A mechanistic study of lambdaphage-mediated recombination in E. coli ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B35321854.
Texte intégralSule, Preeti. « Phenotypic and Genotypic Effects of FlhC Mediated Gene Regulation in Escherichia Coli O157:H7 ». Diss., North Dakota State University, 2011. https://hdl.handle.net/10365/29205.
Texte intégralBendezu, Felipe Oseas. « CELL SHAPE DETERMINATION IN ESCHERICHIA COLI ». Case Western Reserve University School of Graduate Studies / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=case1215459479.
Texte intégralLeong, Mei-kid, et 梁美潔. « Development of an effective method to tag escherichia coli chromosomalgenes by recombineering ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B30430811.
Texte intégral黃雅誼 et Nga-yi Queenie Wong. « DNA engineering utilizing thymidylate synthase A (thyA) selection system in Escherichia coli ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31226851.
Texte intégralPaton, Adrienne Webster. « Molecular characterization of variant shiga-like toxin genes of Escherichia coli / ». Title page, contents and abstract only, 1993. http://web4.library.adelaide.edu.au/theses/09PH/09php3118.pdf.
Texte intégralCrickmore, Neil. « Analysis of a cell division gene cluster in "Escherichia coli" ». Thesis, University of Warwick, 1987. http://wrap.warwick.ac.uk/2468/.
Texte intégralAnton, I. A. « Studies on the shikimate dehydrogenase gene of Escherichia coli ». Thesis, University of Glasgow, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.234808.
Texte intégralStanley, Peter. « Studies on the mutator gene mutS of Escherichia coli K-12 ». Thesis, University of Kent, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328271.
Texte intégralRalph, Edward T. « Molecular characterisation of FNR, the anaerobic transcription regulator of Escherichia coli ». Thesis, University of Sheffield, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323194.
Texte intégralMusuri, Periasamy Vigneshbabu. « Co-ordination of replication initiation with transcriptional regulation in Escherichia coli ». Thesis, State University of New York at Buffalo, 2016. http://pqdtopen.proquest.com/#viewpdf?dispub=10013540.
Texte intégralIn Escherichia coli, replication initiates when the DnaA-ATP protein assembles at the origin oriC. Hda protein in complex with the beta sliding clamp protein (? clamp) on DNA, functions in altering the nucleotide bound form of the replication initiator DnaA protein from DnaA-ATP to DnaA-ADP in a process termed as Regulatory Inactivation of DnaA (RIDA). DnaA also functions as a transcription factor of several genes including the aerobic ribonucleotide reductase nrdAB genes. In this study, I have exploited the cold sensitive growth phenotype due to loss of Hda function and its suppressors to understand the Hda function beyond initiation of replication. I describe the global transcriptional changes in strains lacking Hda and suppressed by two different modes. Loss of Hda function results in reduced expression of nrdAB genes, altered thiol status of the cell, SOS induction and increase in iron import due to de-repression of the Fur regulon. Strains lacking Hda function have increased requirement for the RpoH mediated heat shock response that affects the activity of NrdAB. I have shown that oversupply of ? clamp results in a slow growth phenotype which is more pronounced at low temperatures. Six mutant ? clamps suppress this slow growth phenotype. One of the mutant clamp that has the E202K mutation displays a hyper-Hda phenotypes such as hydroxyurea resistance and increase in nrdAB expression. These phenotypes were dependent on Hda but independent of SOS response. Finally, the slow growth phenotype due to overexpression of ? clamp can be compensated by co-overproducing Hda. This leads to a model where ? clamp could recruit Hda as a response to replication defects independent of SOS response.
Dorrell, Nicholas. « Characterisation of a gene for photorepair in Escherichia coli K-12 ». Thesis, University of Bath, 1993. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.357241.
Texte intégralAgostinho, Juliana Maria Avanci. « Diversidade genética, fatores de virulência e perfis de susceptibilidade a antimicrobianos de isolados de Escherichia coli provenientes do útero, da boca e das fezes de cadelas com piometra / ». Jaboticabal, 2013. http://hdl.handle.net/11449/108413.
Texte intégralBanca: Janete Apparecida Desidério
Banca: Maria de Fátima Martins
Resumo: A piometra canina é uma enfermidade caracterizada pela inflamação do útero com acúmulo de exsudatos, acometendo principalmente fêmeas adultas. Ocorre na fase lútea do ciclo estral em decorrência de alterações hormonais e infecção bacteriana. É reconhecida como uma das principais causas de morte em cadelas e a Escherichia coli é o principal patógeno associado a esta doença. O objetivo deste estudo foi isolar e identificar cepas de E. coli provenientes de conteúdo intra uterino, boca e fezes de cadelas diagnosticadas com piometra, estudar a prevalência de genes codificadores de fatores de virulência uropatogênicos das cepas obtidas testando ainda a semelhança genética entre as cepas isoladas de conteúdo intra uterino e boca e avaliar a susceptibilidade "in vitro" das bactérias isoladas frente a 12 agentes antimicrobianos. Setenta cepas de E. coli, 25 provenientes do conteúdo intra uterino, 26 provenientes da boca e 19 provenientes das fezes, isoladas de 6 cadelas foram examinadas por PCR. Entre as cepas examinadas, 67 (95,7%) foram positivas para o gene fim, 19 (27,1%) foram positivas para iss, 18 (25,7%) foram positivas para hly , 13 (18,5%) foram positivas para iuc e 12 (17,1%) foram positivas para usp. Três animais apresentaram grande similaridade entre as cepas de E. coli isoladas do conteúdo intra uterino e da boca, através da análise da diversidade genética realizada mediante emprego da técnica REP-PCR, ERIC-PCR e BOX-PCR. Resistência antimicrobiana predominante foi detectada para cefalotina (67,1%), ampicilina (65,7%), tetraciclina (61,4%) e nitrofurantoina (58,5%) entre as cepas isoladas. Resistência a múltiplos antimicrobianos foi detectada em 12 (48,0%) dos isolados do conteúdo intra uterino, em 22 (84,6%) dos isolados da boca e em 14 (73,6%) dos isolados das fezes...
Abstract: Canine pyometra is a disease characterized by the inflammation of the uterus with accumulation of purulent discharge, affecting mainly adult animals. Occurs in the luteus phase of the estrus cycle in result of hormone alterations and generally is associated with bacterial infections. It is recognized as one of the main causes of disease and death in the bitch and Escherichia coli is the major pathogen associated with this disease. The aim of this study was to research, isolation and identification of Escherichia coli strains from intrauterine contents, mouth and feces of bitches diagnosed with pyometra, studying the prevalence of uropathogenic virulence factors genes in strains isolated, still testing the genetic similarity among strains isolated from intrauterine contents and mouth and evaluate the susceptibility "in vitro" of the isolated bacteria to 12 antimicrobial drugs. Seventy E. coli strains, 25 from intrauterine contents, 26 from mouth and 19 from feces isolated from six bitches were examined by PCR. Among the strains 67 (95.7%) were positive for fim, 19 (27.1%) were positive for iss, 18 (25.7%) were positive for hly, 13 (18.5%) were positive for iuc and 12 (17.1%) were positive for usp. Multiple antimicrobial resistance was detected for cephalothin (68.0%), nalidixic acid (56.0%) and ampicillin (56.0%) among the pyomera E. coli isolates. Multidrug resistance was found in 12 (48.0%) of the isolates from pyometra, in 22 (84.6%) of the isolates from mouth and in 14 (73.6%) of the isolates from feces...
Mestre
Leung, Hang-mei Polly, et 梁杏媚. « Epidemiology and molecular genetics of verocytotoxigenic escherichia coli in Hong Kong ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31245663.
Texte intégralKempsell, K. « The regulation of glutamate and melibiose utilisation in Escherichia coli ». Thesis, University of Aberdeen, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384465.
Texte intégralBlakely, Garry William. « The regulation and stability of insertion sequence IS1 in Escherichia coli K12 ». Thesis, Queen's University Belfast, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335291.
Texte intégralWalters, Robin George. « The organisation, expression and function of the UVRC gene of Escherichia coli ». Thesis, University of Liverpool, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.237558.
Texte intégralMcFarlane, Ramsay James. « Molecular mechanisms of recombinogenic recircularization of linear plasmid DNA in escherichia coli ». Thesis, University of Liverpool, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384995.
Texte intégralMoffat, K. G. « The molecular cloning of the mutT gene of Escherichia coli K-12 ». Thesis, Cranfield University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373991.
Texte intégralHuen, Shing-yan Michael. « A mechanistic study of lambdaphage-mediated recombination in E. coli ». Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B35321854.
Texte intégralWendel, Brian Michael. « Completion of DNA Replication in Escherichia coli ». PDXScholar, 2018. https://pdxscholar.library.pdx.edu/open_access_etds/4406.
Texte intégralNelson, Adam Michael. « Genomic analysis of pathogen evolution virulence gene acquisition and genetic erosion in Escherichia coli / ». Diss., Connect to online resource - MSU authorized users, 2008.
Trouver le texte intégralBenson, Fiona Elizabeth. « Molecular cloning and analysis of the ruv gene of Escherichia coli K12 ». Thesis, University of Nottingham, 1988. http://eprints.nottingham.ac.uk/30593/.
Texte intégralLuo, Xuebin. « Structural Analysis of the Genes Encoding the Oxalocrotonate Branch of the Pseudomonas putida TOL Plasmid pDKI meta-cleavage Pathway and the Expression of the xy1G Gene Product in Escherichia coli ». Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc500659/.
Texte intégralBaertlein, Dawn Marie August. « Identification of phosphate starvation inducible mineral phosphate solubilization genes in Escherichia coli ». Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184606.
Texte intégralOssanna, Nina. « Isolation and characterization of SOS constitutive mutations in Escherichia coli ». Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184398.
Texte intégralLozano, Gabriela Cazonato. « Avaliação do papel de genes envolvidos na mobilização de poli-3-hidroxibutirato em linhagens recombinantes de Escherichia coli ». Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-17052014-093733/.
Texte intégralThe P3HB is a type of polyester synthesized by bacteria like carbon and energy source, and is mobilizated when there is a shortage of these. A study with mutants of Burkholderia sacchari, showed the involvement of PhaZa1 and LonA in mobilization of P3HB. The present study evaluated the role of PhaZa1 and LonA in this process. The heterologous complementation of mutants of B. sacchari, with phaZa1 and lonA genes from Ralstonia eutropha, reestablished the mobilization capacity in these strains, confirming the involvement of their products in this process. From the recombinant strain of Escherichia coli, harboring accumulation and mobilization genes from R. eutropha, we obtained strains harboring phaZa1 and lonA genes singly and another harboring both genes simultaneously. When expressed singly, both phaZa1 as lonA, had no significant role in mobilization but, when both genes were expressed simultaneously, the rates of mobilization reached more than 50%, appointing that an interaction must occur between PhaZa1 and LonA for the mobilization process to be effective.
Whitby, Matthew Conway. « Molecular and biochemical analysis of the recR operon of Escherichia coli K-12 ». Thesis, University of Nottingham, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335405.
Texte intégralHufton, Simon Evan. « A structure-function analysis of the Escherichia coli vitamin Bâ†1â†2 receptor ». Thesis, University of Nottingham, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317422.
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