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Littérature scientifique sur le sujet « Engineering – Sweden »

Auteur : Grafiati

Publié le 10 mars 2023

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Articles de revues sur le sujet "Engineering – Sweden"

GUSTAFSSON, LYNN. « Engineering Education in Sweden ». European Journal of Engineering Education 18, n^o 1 (janvier 1993) : 77–80. http://dx.doi.org/10.1080/03043799308923212.

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Persson, L. « Engineering geology of Stockholm, Sweden ». Bulletin of Engineering Geology and the Environment 57, n^o 1 (29 juin 1998) : 79–90. http://dx.doi.org/10.1007/s100640050024.

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Webster, J. « Around the world - biomedical engineering in Sweden ». IEEE Engineering in Medicine and Biology Magazine 24, n^o 6 (novembre 2005) : 6–11. http://dx.doi.org/10.1109/memb.2005.1549718.

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Strandberg, Tom. « Raising Visibility of Systems Engineering in Sweden ». INSIGHT 2, n^o 1 (mars 1999) : 27. http://dx.doi.org/10.1002/inst.19992127b.

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Gaidi, Khalid El. « Reforming Engineering Education ». Industry and Higher Education 17, n^o 6 (décembre 2003) : 431–34. http://dx.doi.org/10.5367/000000003322776343.

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The CDIO initiative began as a joint endeavour involving four engineering schools in Sweden and the USA. The number of schools joining the project is increasing constantly. CDIO focuses on four activities – curriculum development, teaching and learning, workshops, and assessment projects – with a view to improving engineering education programmes by redesign and through the introduction of real engineering skills as well as hands-on experiences.

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Östlund, David. « “Laissez-faire under a bell jar” Marquis Childs and the Sweden-fad of the Roosevelt Era ». Scandinavistica Vilnensis, n^o 9 (20 décembre 2014) : 181–99. http://dx.doi.org/10.15388/scandinavisticavilnensis.2014.9.14.

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For decades “Swedology” was a rich and polemically charged genre. “Swedophiles” and “Swedoclasts” were quite as eager to deploy images of Sweden as weapons in foreign contexts as they were interested in the country as such. A telling example is the genre’s early classic, Marquis W. Childs’s Sweden: The Middle Way from 1936. With the backdrop of Dwight Eisenhower’s attempt to get back at Childs by branding Sweden as an extreme society in 1960, this essay aims to see Childs’s book as an argument in its original context during the New Deal. Rather than initiating the 1930s’ American wave of Swedophilia, Childs phrased his argument as an implicit polemic against its apparent exaggerations. Sweden was not a Utopia; the point in studying its example was on the contrary the pragmatism shown in the Swedes’ attempts to solve everyday problems in a reasonable and genuinely democratic way, negotiating and compromising. As a text implicitly supporting the Roosevelt agenda, Childs’s book was far from encouraging federal dirigisme, expert rule and central planning (“social engineering”): on the contrary the message to other New Dealers was to shun such things in favor of grass-roots activities and initiatives. The predominant theme – the consumers’ cooperation movement’s central role in counteracting monopolies, thus creating economic efficiency and turning Sweden into the world’s only truly working laissez-faire economy – harmonized with Childs’s commitment to projects like the federal rural electrification program, which in a “Swedish” manner was founded on co-ops and a vision of popular self-determination.

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Antoni, Nils. « Research perspective : Sweden ». Batiment International, Building Research and Practice 14, n^o 3 (mai 1986) : 175–79. http://dx.doi.org/10.1080/01823328608726740.

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Olav-Fornell, Jan. « SiC in Sweden ». III-Vs Review 8, n^o 1 (février 1995) : 34–36. http://dx.doi.org/10.1016/0961-1290(95)80156-1.

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Stephansson, O. « Rock mechanics in Sweden ». International Journal of Rock Mechanics and Mining Sciences & ; Geomechanics Abstracts 26, n^o 1 (janvier 1989) : 1–11. http://dx.doi.org/10.1016/0148-9062(89)90520-2.

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WOLMING, SIMON. « ADMISSION TO UNIVERSITY ENGINEERING PROGRAMS IN SWEDEN : A MULTIPURPOSE APPROACH ». Psychological Reports 94, n^o 3 (2004) : 1125. http://dx.doi.org/10.2466/pr0.94.3.1125-1126.

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Thèses sur le sujet "Engineering – Sweden"

Cacija, Goran, et Christofer Lindberg. « Angels of Sweden ». Thesis, Halmstad University, Mechanical Engineering and Industrial Design (MTEK), 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-4651.

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Arbetet inleddes med ett möte med Evonova där projektet diskuterades. Företagets inledandetankegångar och idéer kring produkten framgick och arbetet påbörjades.Under arbetets gång följdes Fredy Olssons anvisningar i hur en teknisk rapport ska se ut. Alltinleddes med en brainstorm och kundundersökning. Kundundersökningens resultat mynnadeut i en kravprofil, till denna tillkom även Evonovas krav och önskningar. Kravprofilenanvändes när vi sållade ut lösningar från brainstormen.Vidare använde vi oss av idéerna vi fått fram och arbetade fram CATIA-modeller på möjligaförslag. Dessa mättes, gjordes om samt blev utvärderade av företaget. Enbart två modeller,hjärtat och droppen, ansågs tillräckligt tilltalande av uppdragsgivaren.Den slutgiltiga lösningen gjordes det en prototyp av som togs till företaget och UTEXPO.

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Pérez, Martín Beatriz. « Nursery in Halmstad (Sweden) ». Thesis, Högskolan i Halmstad, Sektionen för ekonomi och teknik (SET), 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-14674.

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A construction project including planning, cost estimation and design of a ”Nursery school” on a property in the city of Halmstad.
Ett byggnadsprojekt med planering, kostnadsberäkning och projektering av en ”Nursery school” på en fastighet i Halmstad.

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Rydin, Wiktoria, et Isabelle Andersson. « Logistic solutions Slovakia - Sweden ». Thesis, Tekniska Högskolan, Högskolan i Jönköping, JTH, Maskinteknik, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-41216.

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This report is a bachelor thesis project conducted by Isabelle Andersson and Wiktoria Rydin as a part of the education Mechanical Engineering, Industrial Economy and Production Management, at the School of Engineering, Jönköping University. The project is a collaboration with Hörle Wire Group AB, a company that produces round and flat-rolled wire of high-quality and they belong to one of Europe's leading suppliers of wire. The purpose of the bachelor theses was to review Hörle Wire's transport solutions from their facility in Nitra, Slovakia, to their two main customers in Sweden. Hörle Wire has a problem with gaining profitability regarding these transports. The cost of transporting the goods from Nitra to the customers is so high that the profit margin becomes very low. The problem was studied through qualitative and quantitative data collection in the form of interviews with staff at Hörle Wire and with their customers. The resulted in a clear picture of the emerging problem, and with the theories and facts studied in the field, proposals could be processed. Based on this, some suggestions for improvements were made that Hörle Wire could apply in its efforts to affect the transport. Some different proposals have been made, for example completely changing the means of transport, changing the appearance of today's transportation and to establish a warehouse.

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Larsen, Joakim, et Tobias Tunestam. « Dimensioning of a Residential Microgrid in Sweden ». Thesis, KTH, Industriell ekologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-210742.

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This project aims to evaluate through simulationsin Simulink whether an average power consuming, 4-person household in Sweden, can be successfully supplied from a microgrid system powered by solar panels. The goal is to achievea 48 V DC system and examine both its voltage stability and long term power balance for stationary and transient conditions. The simulations are based on weather data such as temperature and solar irradiation as well as an estimated consumption pattern fort he household. Loads and sources in the system are identified as well as possible algorithms for communication between nodes. Two scenarios are tested, a stand alone grid with optional energy storage and a system connected to the main grid. The results indicate that a Swedish household can not be supplied from a stand alone microgrid due to low irradiation values, without an unreasonably large energy storage or change in consumption. Although a continuous power supply is missing, voltage stability and power balance is established for this scenario. With connection to the main grid all requirements above are met. The observed limitations of the methodology are analyzed. For future projects a communication algorithm for load variation, synchronized with irradiation inputs, can be implemented in the model. Also, simulations can be done for alternative geographical conditions to yield different results where alternative renewablesources, such as wind and geothermal, can be included.

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Huang, Wenting. « IPR Barriers in Collaboration between University and Engineering Industry in Sweden ». Thesis, KTH, Samhällsekonomi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-48720.

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This thesis examines the barriers, especially intellectual property rights concerned that inhibit industry academia collaboration. By analyzing Swedish firms in the engineering industry, I explore the influence of IPR barrier on firms’ benefits, short- and long-term respectively from university-industry interaction. Three hypotheses are suggested to investigate the relationship between IPR barriers, firm categories, short-term benefits and long-term benefits. The results illustrate different firms’ reactions to IPR barriers. According to the analysis, advanced firms are more likely to report IPR barriers. Moreover, they tend to benefit more in the long run. However, the short-term benefits from UI collaboration seem to have no significant relationship with IPR barriers. There is hence no proof in the data that IPR barriers are a significant hinder for firms to benefit from university-industry interaction.

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Abdallah, Ali. « The perception of Nuclear powerin Sweden : How engineering students view it ». Thesis, Uppsala universitet, Industriell teknik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-335139.

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Nuclear power has been an important part of the electricity generatingin Sweden in almost half a century. However, the use of nuclear powerraises many complex issues such as economy, accident risks, andradioactive waste management and storage. Nuclear power has thereforebeen debated intensively in Sweden as in many other countries since the1970s. Several countries have changed their nuclear policy and starteddecommissioning programs following the nuclear accident in Fukushimaback in 2011. Citizens' support for nuclear power has declined worldwidein last few years, including in Sweden. This study aims to investigatethe perception of nuclear power among engineering students and showsthat the majority of engineering students, who generally are positivetowards technology, have been affected by their surrounding and areagainst nuclear power. Engineering students in general, both supportersand opponents of nuclear power, agree that Sweden should gradually phaseout nuclear power and replace it by renewable energy sources.

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Lopez, Lopez Mikel. « FINAL PROJECT-Public Library, Halmstad, Sweden 2011 ». Thesis, Högskolan i Halmstad, Sektionen för ekonomi och teknik (SET), 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-15531.

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Molavi, Sam, et William Bydén. « Solar Prosumer Business Models in Sweden ». Thesis, KTH, Skolan för industriell teknik och management (ITM), 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-232569.

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In this research, four of the most common business models for Swedish photovoltaic (PV) prosumers were analyzed through a business case study. The costs and revenues related to adopting these prosumer business models for a household were examined and their economic viability was measured using the Net Present Value- and Payback method. The markets of PV and Battery Energy Storage Systems (BESS) in Sweden were researched in order to provide an overview of the potential for future prosumers in Sweden. Relevant actors and business models have been described and analyzed. Furthermore, Sweden’s energy structure as a whole and its potential transformation to a more decentralized and prosumer friendly system has been discussed. The business case study results show that the most economically beneficial business model for prosumers today in Sweden is through a Power Purchase Agreement (PPA). The business model where the prosumer owns a PV system proved to be the second best option which also was calculated to be economically beneficial. Leasing a PV system or owning a combined PV- and BESS were proven to not be economically beneficial.
I den här rapporten har fyra av de vanligaste affärsmodellerna för Svenska solcells-prosumers analyserats genom en business case study. Kostnaderna och intäkterna kopplade till att uppta dessa affärsmodeller för ett hushåll har undersökts och deras ekonomiska duglighet har mätts med nuvärdesmetoden och återbetalningsmetoden. Marknaderna för solceller och energilagringssystem av batterier i Sverige har undersökts för att ge en översikt av möjligheterna för framtida prosumers i Sverige. Relevanta aktörer och affärsmodeller har beskrivits och analyserats. Vidare har Sveriges energistruktur och dess potentiella övergång till ett mer decentraliserat och prosumervänligt system diskuterats. Resultaten från vår business case study visar att den mest ekonomiskt lönsamma affärsmodellen för prosumers idag i Sverige är genom ett energiköpeavtal. Affärsmodellen att ha ett egenägt solcellssystem var det näst bästa alternativet och visade sig också vara ekonomiskt lönsamt. Att hyra ett solcellssystem eller att äga ett solcellsystem i kombination med ett energilagringssystem av batterier visade sig vara olönsamma ur ett ekonomiskt perspektiv.

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Rydhagen, Birgitta. « Feminist Sanitary Engineering as a Participatory Alternative in South Africa and Sweden ». Doctoral thesis, Karlskrona : Blekinge Institute of Technology, 2002. http://www.bth.se/fou/forskinfo.nsf/allfirst2/0ecaa05e81bfec0ec1256fbd0057a98b?OpenDocument.

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Holm, Jenny. « Transnational career agents : A comparative study on international engineering students in Sweden ». Thesis, Stockholms universitet, Institutionen för pedagogik och didaktik, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-181005.

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The mobility of students has increased dramatically in the past decades which primarily is a consequence of globalization (Ninnes & Hellstén, 2005). Many higher education institutions (HEIs) around the world have responded to the global educational trend by actively recruiting students from abroad, with the incentive of maintaining a competitive position on the global, knowledge-based market (Knight, 2004). Simultaneously, an increasing number of students have responded to the expanded opportunities which have become available, aspiring to secure the best education to facilitate their path into a well-remunerated career (Waters & Brooks, 2011). By employing a qualitative research approach, this study explores how international degree-seeking students undertaking engineering studies in Swedish HE, take on their career development within a global framework. The findings are compared and analyzed in order to provide further insights into the career trajectories of engineering students from different countries. The findings suggest that the participants in the study have employed a large degree of agency and independence in advancing their career development further. Moreover, it has also been indicated that they, as global professionals, uphold a flexible stance towards future career opportunities, regardless of where in the world these would be located. Considering the increasing numbers of international students that occupy Swedish HEIs, the study concludes that further attention needs to be paid to gain further insights into the realities of this student population, both to increase our understanding of how they respond to the forces of globalization and to safeguard that career services address the real career needs of this population.

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Livres sur le sujet "Engineering – Sweden"

Alva and Gunnar Myrdal : Social engineering in the modern world. Lanham : Lexington Books, 2014.

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Wield, David. Forging the links : Evaluation of SAREC support to research development in engineering and sciences in Mozambique. [Stockholm?] : Swedish Agency for Research Cooperation with Developing Countries, 1991.

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IEEE/NASA Software Engineering Workshop (33rd 2009 Skövde, Sweden). Proceedings, 33rd Annual IEEE Software Engineering Workshop : 13-14 October, 2009, Skövde, Sweden. Los Alamitos, Calif : IEEE Computer Society, 2010.

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Sweden) International RILEM Symposium on Timber Engineering (1st 1999 Stockholm. 1st International RILEM Symposium on Timber Engineering : Stockholm, Sweden, September 13-14, 1999. Cachan [France] : RILEM publications, 1999.

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Stockholm, Sweden) Workshop on Human Reliability Models : Theoretical and Practical Challenges (1994. Proceedings of Workshop on Human Reliability Models Theoretical and Practical Challenges : Stockholm, Sweden, August 22-24, 1994. College Park, MD : Center for Reliability Engineering, University of Maryland, 1998.

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IEEE International Conference on Engineering of Complex Computer Systems (7th 2001 Skövde, Sweden). Seventh IEEE International Conference on Engineering of Complex Computer Systems : Proceedings : Skövde, Sweden, June 11-13, 2001. Los Alamitos, Calif : IEEE Computer Society, 2001.

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(Sweden), Statens geotekniska institut, dir. SGI 1944-1994, Statens geotekniska institut. Linköping : Statens geotekniska institut, 1994.

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Karlsson, Lennart. Mechanical Effects of Welding : IUTAM Symposium, Luleå/Sweden, June 10-14, 1991. Berlin, Heidelberg : Springer Berlin Heidelberg, 1992.

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CAiSE, '90 (1990 Stockholm Sweden). Advanced information systems engineering : Second Nordic conference, CAiSE '90, Stockholm, Sweden, May 8-10, 1990 : proceedings. Berlin : Springer-Verlag, 1990.

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Singh, Val. Gender and managerial meanings of commitment in high tech engineering in the UK and Sweden. Cranfield : Cranfield University, 1998.

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Chapitres de livres sur le sujet "Engineering – Sweden"

Malmqvist, Johan, et Aija Sadurskis. « Quality Assurance of Engineering Education in Sweden ». Dans Engineering Education Quality Assurance, 133–43. Boston, MA : Springer US, 2009. http://dx.doi.org/10.1007/978-1-4419-0555-0_10.

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Etzemüller, Thomas. « Rationalizing the Individual – Engineering Society : The Case of Sweden ». Dans Engineering Society, 97–118. London : Palgrave Macmillan UK, 2012. http://dx.doi.org/10.1057/9781137284501_5.

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Prawitz, Jan. « From Nuclear Option to Non-nuclear Promotion : The Sweden Case ». Dans Pioneers in Arts, Humanities, Science, Engineering, Practice, 61–79. Cham : Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-12797-7_6.

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Erlingsson, Sigurður, et Denis Saliko. « Correlating Air Freezing Index and Frost Penetration Depth—A Case Study for Sweden ». Dans Lecture Notes in Civil Engineering, 847–57. Cham : Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-48679-2_79.

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Brander, Linus, et Björn Schouenborg. « CE-Marking of Natural Stone—Practical Application and Solutions in Sweden ». Dans Engineering Geology for Society and Territory - Volume 5, 309–12. Cham : Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-09048-1_59.

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Liu, Zhendong, Sebastian Stichel, Peter Larsson et Jan Hjort. « Study on the Characterization of Degraded Catenary in Sweden to Enhance Safety and Reliability ». Dans Lecture Notes in Mechanical Engineering, 212–19. Cham : Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-38077-9_26.

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Casanueva, Carlos, Babette Dirks et Tohmmy Bustad. « Rail Vehicle Dynamics Simulation-Based Decision Support for Novel Block Brake Material Implementation in Sweden ». Dans Lecture Notes in Mechanical Engineering, 775–82. Cham : Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-38077-9_90.

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Kans, Mirka, et Lasse Metso. « Maintenance Education in Engineering Programs on Bachelor and Master Level : Evidence from Finland and Sweden ». Dans Engineering Assets and Public Infrastructures in the Age of Digitalization, 465–74. Cham : Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-48021-9_52.

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Gröndahl, Fredrik, et Daniel Franzen. « A Practical Approach to Integrating Research and Education : A Course Experiment from KTH, Sweden ». Dans New Developments in Engineering Education for Sustainable Development, 69–79. Cham : Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-32933-8_7.

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Kettunen, Petri, Tomas Gustavsson, Maarit Laanti, Andreas Tjernsten, Tommi Mikkonen et Tomi Männistö. « Impacts of COVID-19 Pandemic for Software Development in Nordic Companies – Agility Helps to Respond ». Dans Agile Processes in Software Engineering and Extreme Programming – Workshops, 33–41. Cham : Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-88583-0_4.

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AbstractIn 2020, the global COVID-19 pandemic affected almost every company in some way also in the Nordic countries. Depending on the different industry sectors of the companies, the impacts have varied from minor risks to severe disruptions but also even booming businesses. In all, agility and resilience have been required to continue and even to survive. In 2018, we started conducting large-scale agile surveys in Finland and Sweden. For the 2020 survey round, we included questions about the current pandemic situation impacts and how agility has helped to respond. The respondents represented software professionals from different industries, not limited to information and communication technology (ICT) companies. The results indicate that although the perceived impacts have mostly been negative (53%), it is not all so. One-third (33%) reported positive impacts such as increased business and better well-being. The majority (55%) of the responses indicated that agility has helped to respond to the pandemic situation. Remarkably, 59% reported that their companies have improved agility during the past year. Improved agility appears to be positively related to the ability to respond to the pandemic. We did not discover significant differences between the Finnish and Swedish respondent cohorts.

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Actes de conférences sur le sujet "Engineering – Sweden"

Wall, Göran. « Tools for Sustainable Energy Engineering ». Dans World Renewable Energy Congress – Sweden, 8–13 May, 2011, Linköping, Sweden. Linköping University Electronic Press, 2011. http://dx.doi.org/10.3384/ecp110572323.

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Oberg, P. A. « Trends in clinical engineering in Sweden ». Dans 1992 14th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 1992. http://dx.doi.org/10.1109/iembs.1992.5761706.

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Oberg. « Trends In Clinical Engineering In Sweden ». Dans Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 1992. http://dx.doi.org/10.1109/iembs.1992.593808.

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Ariyanfar, L., H. Ghadamian et R. Roshandel. « Alkaline Fuel Cell (AFC) Engineering Design, Modeling and Simulation for UPS Provide in Laboratory Application ». Dans World Renewable Energy Congress – Sweden, 8–13 May, 2011, Linköping, Sweden. Linköping University Electronic Press, 2011. http://dx.doi.org/10.3384/ecp110571227.

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Stephens, Sutton F. « Faculty Lead Study Abroad : Wood Engineering in Sweden ». Dans Structures Congress 2008. Reston, VA : American Society of Civil Engineers, 2008. http://dx.doi.org/10.1061/41016(314)141.

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Eriksson, Sture J. A. « The Electrical Power Engineering Profession in Sweden - Overview and Milestones ». Dans 2009 IEEE Conference on the History of Technical Societies. IEEE, 2009. http://dx.doi.org/10.1109/hts.2009.5337820.

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Adlemo, Anders, He Tan et Vladimir Tarasov. « Test case quality as perceived in Sweden ». Dans ICSE '18 : 40th International Conference on Software Engineering. New York, NY, USA : ACM, 2018. http://dx.doi.org/10.1145/3195538.3195541.

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Täljsten, Björn, Thomas Blanksvärd, Gabriel Sas, Niklas Bagge, Jonny Nilimaa, Cosmin Popescu, Lennart Elfgren, Anders Carolin et Jens Häggström. « Bridges Tested to Failure in Sweden ». Dans IABSE Conference, Copenhagen 2018 : Engineering the Past, to Meet the Needs of the Future. Zurich, Switzerland : International Association for Bridge and Structural Engineering (IABSE), 2018. http://dx.doi.org/10.2749/copenhagen.2018.064.

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Five bridges of different types have been tested to failure and the results have been compared to analyses of the load-carrying capacity using standard code models and advanced numerical methods. The results may help to make accurate assessments of similar existing bridges. There it is necessary to know the real behaviour, weak points, and to be able to model the load-carrying capacity in a correct way.<p> The five bridges were: (1) a strengthened one span concrete road bridge - Stora Höga ; (2) a one span concrete rail trough bridge loaded in fatigue – Lautajokk; (3) a two span strengthened concrete trough railway bridge - Övik; (4) a one span railway steel truss bridge -Åby; and (5) a five span prestressed concrete road bridge - Kiruna. The unique results in the paper are the experiences of the real failure types, the robustness/weakness of the bridges, and the accuracy and shortcomings/potentials of different codes and models for safety assessment of existing structures.

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Mikkelsen, Peter S., Maria Viklander, Jens J. Linde et Per-Arne Malmqvist. « BMPs in Urban Stormwater Management in Denmark and Sweden ». Dans Engineering Foundation Conference 2001. Reston, VA : American Society of Civil Engineers, 2002. http://dx.doi.org/10.1061/40602(263)24.

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Stahre, Peter. « Recent Experiences in the Use of BMPs in Malmö, Sweden ». Dans Engineering Foundation Conference 2001. Reston, VA : American Society of Civil Engineers, 2002. http://dx.doi.org/10.1061/40602(263)16.

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Rapports d'organisations sur le sujet "Engineering – Sweden"

Lewinsohn, Efraim, Eran Pichersky et Shimon Gepstein. Biotechnology of Tomato Volatiles for Flavor Improvement. United States Department of Agriculture, avril 2001. http://dx.doi.org/10.32747/2001.7575277.bard.

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The main objectives of the research project were: 1. The manipulation, by genetic engineering techniques, of the terpenoid pathway in tomato fruit. Specifically, to test the hypothesis whether overexpression of linalool synthase in tomato fruits will result in the diversion of intermediates of the carotene biosynthetic pathway to linalool, demonstrating that linalool synthase is a key regulatory enzyme, and possibly improving tomato flavor. 2. The elucidation of the biochemical pathway leading to eugenol and methyl eugenol, and the manipulation of this pathway to determine key enzymes and to improve flavor in tomato. Background, conclusions and implications The different proportions of volatile components present in foods often determine their flavor properties. Two of the ten most important flavor compounds in tomatoes, linalool and eugenol, are emitted by the flowers of Clarkia breweri, (Onagraceae), a plant native to California, and are also present in sweet basil (Ocimum basilicum, Lamiaceae). We have studied the key enzymes and genes involved in the production of these flavorants. Linalool synthase, the key enzyme in linalool biosynthesis and its corresponding gene were isolated and characterized from Clarkia breweri. The gene was coupled to a fruit-specific tomato promotor (E8) and was used to transform tomatoes. The transgenic tomatoes produced S-linalool and 1-hydroxylinalool, compounds absent from the fruits of controls. The transgenesis did not adversely affect the overall appearance of the plants nor the levels of other terpenoids present such as carotenoids and vitamin E. Our work has proven that the terpenoid pathway in tomatoes can be modified by the introduction and expression of foreign genes coding for the enzymes controlling the production of monoterpenoid flavor compounds. We have also isolated novel enzymes and genes that are involved in the formation of eugenol and methyl eugenol from Clarkia breweri and basil. An EST library of basil glandular trichomes (the site of eugenol and methyl eugenol biosynthesis) was prepared. More than 1,200 genes have been preliminary characterized and a few of them have been confirmed by functional expression, to be involved in eugenol and methyl eugenol biosynthesis. These genes have augmented the still small repertoire of genes that are available to modify the aroma of agricultural produce by genetic engineering.

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Coplin, David, Isaac Barash et Shulamit Manulis. Role of Proteins Secreted by the Hrp-Pathways of Erwinia stewartii and E. herbicola pv. gypsophilae in Eliciting Water-Soaking Symptoms and Initiating Galls. United States Department of Agriculture, juin 2001. http://dx.doi.org/10.32747/2001.7580675.bard.

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Many bacterial pathogens of plants can inject pathogenicity proteins into host cells using a specialized type III secretion system encoded by hrpgenes. This system deliver effector proteins, into plant cells that function in both susceptible and resistant interactions. We have found that the virulence of Erwinia stewartii(Es; syn. Pantoea stewartii) and Erwinia herbicola pv. gypsophilae (Ehg, syn. Pantoea agglomerans), which cause Stewart's wilt of corn and galls on Gypsophila, respectively, depends on hrpgenes. The major objectives of this project were: To increase expression of hrpgenes in order to identify secreted proteins; to identify genes for proteins secreted by the type-III systems and determine if they are required for pathogenicity; and to determine if the secreted proteins can function within eukaryotic cells. We found that transcription of the hrp and effector genes in Es and Ehg is controlled by at least four genes that constitute a regulatory cascade. Environmental and/or physiological signaling appears to be mediated by the HrpX/HrpY two component system, with HrpX functioning as a sensor-kinase and HrpY as a response regulator. HrpYupregulateshrpS, which encodes a transcriptional enhancer. HrpS then activates hrpL, which encodes an alternate sigma factor that recognizes "hrp boxes". All of the regulatory genes are essential for pathogenicity, except HrpX, which appears only to be required for induction of the HR in tobacco by Es. In elucidating this regulatory pathway in both species, we made a number of significant new discoveries. HrpX is unusual for a sensor-kinase because it is cytoplasmic and contains PAS domains, which may sense the redox state of the bacterium. In Es, a novel methyl-accepting protein may function upstream of hrpY and repress hrp gene expression in planta. The esaIR quorum sensing system in Es represses hrp gene expression in Es in response to cell-density. We have discovered six new type III effector proteins in these species, one of which (DspE in Ehg and WtsE in Es) is common to both pathogens. In addition, Es wtsG, which is a homolog of an avrPpiB from P. syringae pv. pisi, and an Ehg ORF, which is a homolog of P. syringae pv. phaseolicola AvrPphD, were both demonstrated to encode virulence proteins. Two plasmidborne, Ehg Hop proteins, HsvG and PthG, are required for infection of gypsophilia, but interestingly, PthG also acts as an Avr elicitor in beets. Using a calmodulin-dependent adenylate cyclase (cyaA) reporter gene, we were successful in demonstrating that an HsvG-CyaA fusion protein can be transferred into human HeLa cells by the type-III system of enteropathogenic E. coli. This is a highly significant accomplishment because it is the first direct demonstration that an effector protein from a plant pathogenic bacterium is capable of being translocated into a eukaryotic cell by a type-III secretion system. Ehg is considered a limiting factor in Gypsophila production in Israel and Stewart’s Wilt is a serious disease in the Eastern and North Central USA, especially on sweet corn in epidemic years. We believe that our basic research on the characterization of type III virulence effectors should enable future identification of their receptors in plant cells. This may lead to novel approaches for genetically engineering resistant plants by modifying their receptors or inactivating effectors and thus blocking the induction of the susceptible response. Alternatively, hrp gene regulation might also provide a target for plant produced compounds that interfere with recognition of the host by the pathogen. Such strategies would be broadly applicable to a wide range of serious bacterial diseases on many crops throughout the USA and Israel.

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