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Articles de revues sur le sujet « Elastin-Like Polypeptides (ELP) »

Auteur : Grafiati

Publié le 9 mars 2023

Mis à jour le 31 juillet 2025

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1

Tang, Xin. "Elastin-Like Polypeptides as Thermosensitive Polymer System." Advanced Materials Research 898 (February 2014): 296–99. http://dx.doi.org/10.4028/www.scientific.net/amr.898.296.

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Thermo-responsive elastin-like polypeptides (ELPs) were successfully obtained by inverse transition cycling (ITC) and recursive directional ligation (RDL). Six ELPs displayed thermal properties, depending on their sequence and chain length. It was found that the ELP[KV8F-4 and ELP[KV8F-8 were effective as thermosensitive materials at the body temperature with phase transition temperature from 35 to 45oC.

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Lin, Charng-Yu, and Julie C. Liu. "Incorporation of short, charged peptide tags affects the temperature responsiveness of positively-charged elastin-like polypeptides." Journal of Materials Chemistry B 7, no. 34 (2019): 5245–56. http://dx.doi.org/10.1039/c9tb00821g.

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Dai, Michèle, Evangelos Georgilis, Guillaume Goudounet, et al. "Refining the Design of Diblock Elastin-Like Polypeptides for Self-Assembly into Nanoparticles." Polymers 13, no. 9 (2021): 1470. http://dx.doi.org/10.3390/polym13091470.

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Diblock copolymers based-on elastin-like polypeptide (ELP) have the potential to undergo specific phase transitions when thermally stimulated. This ability is especially suitable to form carriers, micellar structures for instance, for delivering active cargo molecules. Here, we report the design and study of an ELP diblock library based on ELP-[M1V3-i]-[I-j]. First, ELP-[M1V3-i]-[I-j] (i = 20, 40, 60; j = 20, 90) that showed a similar self-assembly propensity (unimer-to-aggregate transition) as their related monoblocks ELP-[M1V3-i] and ELP-[I-j]. By selectively oxidizing methionines of ELP-[M1

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Dragojevic, Sonja, Rebecca Mackey, and Drazen Raucher. "Evaluation of Elastin-Like Polypeptides for Tumor Targeted Delivery of Doxorubicin to Glioblastoma." Molecules 24, no. 18 (2019): 3242. http://dx.doi.org/10.3390/molecules24183242.

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To increase treatment efficiency for glioblastoma, we have developed a system to selectively deliver chemotherapeutic doxorubicin (Dox) to Glioblastoma (GBM) tumors. This carrier is based on elastin-like polypeptide (ELP), which is soluble at physiological temperatures but undergoes a phase transition and accumulates at tumor sites with externally applied, mild (40–41 °C) hyperthermia. The CPP-ELP-Dox conjugate consists of a cell penetrating peptide (CPP), which facilitates transcytosis through the blood brain barrier and cell entry, and a 6-maleimidocaproyl hydrazone derivative of doxorubicin

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VerHeul, Ross, Craig Sweet, and David H. Thompson. "Rapid and simple purification of elastin-like polypeptides directly from whole cells and cell lysates by organic solvent extraction." Biomaterials Science 6, no. 4 (2018): 863–76. http://dx.doi.org/10.1039/c8bm00124c.

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Elastin-like peptide (ELP) constructs are typically hydrophobic in nature. A rapid organic solvent extraction method is reported that affords highly pure ELP with removal of key contaminants such as nucleic acids and LPS.

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Shin, Han Bin, and Young Kee Chae. "Aggregation-Dispersion Chromatography: Application of Elastin-like Polypeptides." Separations 11, no. 12 (2024): 335. http://dx.doi.org/10.3390/separations11120335.

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Protein purification is a crucial step for various downstream applications like drug development, antibody preparation, and structure determination. The constant pursuit is for methods that are more efficient and cost-effective. We propose a novel approach using an elastin-like polypeptide (ELP) as an aggregation core that serves as an anchor between the beads in a chromatography column. In this method, a chilled sample containing a [target protein type] fusion protein is loaded onto a pre-equilibrated IMAC (immobilized metal affinity chromatography) column with a low-salt buffer. The column i

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Lee, Sujin, Ji-Eun Kim, Hye-Jin Seo, and Jun-Hyeog Jang. "Design of fibronectin type III domains fused to an elastin-like polypeptide for the osteogenic differentiation of human mesenchymal stem cells." Acta Biochimica et Biophysica Sinica 51, no. 8 (2019): 856–63. http://dx.doi.org/10.1093/abbs/gmz063.

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Abstract Extracellular matrix (ECM) including fibronectin (FN) and elastin plays a pivotal role in providing a microenvironment to support tissue regeneration in stem cell therapy. To develop a novel biomimetic ECM for stem cell differentiation, we engineered FN type III 9 and 10 domains fused to elastin-like polypeptides (FN-ELPs). The recombinant FN-ELP fusion protein was expressed in Escherichia coli and purified by inverse transition cycling. Human mesenchymal stem cells (hMSCs) cultured on plates coated with FN-ELP had significantly greater adhesion activity and proliferation than cells g

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Funtan, Sebastian, Anne Funtan, Reinhard Paschke, and Wolfgang H. Binder. "Biomimetic Elastin-Like Polypeptides as Materials for the Activation of Mechanophoric Catalysts." Organic Materials 02, no. 02 (2020): 116–28. http://dx.doi.org/10.1055/s-0040-1702149.

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Elastin-like polypeptides (ELPs) are well known for their elastic and thermoresponsive behaviors. Their elasticity originates from the formation of a β-spiral which is the consequence of stacking type-II β-turns, formed from individual VPGVG pentapeptide units. Here, the synthesis of ELPs of varying chain lengths [VPGVG, (VPGVG)2, and (VPGVG)4] and their coupling to a mechanoresponsive catalyst are reported. The attached ELP chains can act as “molecular springs,” allowing for an efficient uptake and transmission of an applied force to the mechanophoric bond. This leads to stress-induced activa

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Sugioka, Yusuke, Jin Nakamura, Chikara Ohtsuki, and Ayae Sugawara-Narutaki. "Thixotropic Hydrogels Composed of Self-Assembled Nanofibers of Double-Hydrophobic Elastin-Like Block Polypeptides." International Journal of Molecular Sciences 22, no. 8 (2021): 4104. http://dx.doi.org/10.3390/ijms22084104.

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Physically crosslinked hydrogels with thixotropic properties attract considerable attention in the biomedical research field because their self-healing nature is useful in cell encapsulation, as injectable gels, and as bioinks for three-dimensional (3D) bioprinting. Here, we report the formation of thixotropic hydrogels containing nanofibers of double-hydrophobic elastin-like polypeptides (ELPs). The hydrogels are obtained with the double-hydrophobic ELPs at 0.5 wt%, the concentration of which is an order of magnitude lower than those for previously reported ELP hydrogels. Although the kinetic

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Cobb, Jared S., Anna S. Rourke, Aiden Creel, and Amol V. Janorkar. "Manipulating the solution environment to control the surface roughness of elastin-based polymer coatings." Journal of Biomaterials Applications 36, no. 3 (2021): 419–27. http://dx.doi.org/10.1177/08853282211010302.

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Elastin-like polypeptides (ELP) have been used as a genetically-engineered, biocompatible substitute for elastin. Cell culture coatings prepared using ELP conjugated to low molecular weight polyethyleneimine (PEI) entices cells to form three-dimensional cellular aggregates that mimic their in vivo counterparts. This study seeks to control the deposition of the ELP and ELP-PEI molecules to control the roughness of the final coatings. The two polymers were coated onto three different substrates (glass, polystyrene, tissue-culture polystyrene) and the solution environment was altered by changing

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Wang, Wan, Changrim Lee, Martha Pastuszka, Gordon W. Laurie, and J. Andrew MacKay. "Thermally-Responsive Loading and Release of Elastin-Like Polypeptides from Contact Lenses." Pharmaceutics 11, no. 5 (2019): 221. http://dx.doi.org/10.3390/pharmaceutics11050221.

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Contact lenses are widely prescribed for vision correction, and as such they are an attractive platform for drug delivery to the anterior segment of the eye. This manuscript explores a novel strategy to drive the reversible adsorption of peptide-based therapeutics using commercially available contact lenses. To accomplish this, thermo-sensitive elastin-like polypeptides (ELPs) alone or tagged with a candidate ocular therapeutic were characterized. For the first time, this manuscript demonstrates that Proclear CompatiblesTM contact lenses are a suitable platform for ELP adsorption. Two rhodamin

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Ryu, Jung Su, Felix Kratz, and Drazen Raucher. "Cell-Penetrating Doxorubicin Released from Elastin-Like Polypeptide Kills Doxorubicin-Resistant Cancer Cells in In Vitro Study." International Journal of Molecular Sciences 22, no. 3 (2021): 1126. http://dx.doi.org/10.3390/ijms22031126.

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Elastin-like polypeptides (ELPs) undergo a characteristic phase transition in response to ambient temperature. Therefore, it has been be used as a thermosensitive vector for the delivery of chemotherapy agents since it can be used to target hyperthermic tumors. This novel strategy introduces unprecedented options for treating cancer with fewer concerns about side effects. In this study, the ELP system was further modified with an enzyme-cleavable linker in order to release drugs within tumors. This system consists of an ELP, a matrix metalloproteinase (MMP) substrate, a cell-penetrating peptid

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Le Fer, Gaëlle, Delphine Portes, Guillaume Goudounet, Jean-Michel Guigner, Elisabeth Garanger, and Sébastien Lecommandoux. "Design and self-assembly of PBLG-b-ELP hybrid diblock copolymers based on synthetic and elastin-like polypeptides." Organic & Biomolecular Chemistry 15, no. 47 (2017): 10095–104. http://dx.doi.org/10.1039/c7ob01945a.

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Shi, Xianggang, Dongfeng Chen, Guodong Liu, et al. "Application of Elastin-Like Polypeptide in Tumor Therapy." Cancers 14, no. 15 (2022): 3683. http://dx.doi.org/10.3390/cancers14153683.

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Elastin-like polypeptides (ELPs) are stimulus-responsive artificially designed proteins synthesized from the core amino acid sequence of human tropoelastin. ELPs have good biocompatibility and biodegradability and do not systemically induce adverse immune responses, making them a suitable module for drug delivery. Design strategies can equip ELPs with the ability to respond to changes in temperature and pH or the capacity to self-assemble into nanoparticles. These unique tunable biophysicochemical properties make ELPs among the most widely studied biopolymers employed in protein purification,

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Sugawara-Narutaki, Ayae, Sawako Yasunaga, Yusuke Sugioka, et al. "Rheology of Dispersions of High-Aspect-Ratio Nanofibers Assembled from Elastin-Like Double-Hydrophobic Polypeptides." International Journal of Molecular Sciences 20, no. 24 (2019): 6262. http://dx.doi.org/10.3390/ijms20246262.

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Elastin-like polypeptides (ELPs) are promising candidates for fabricating tissue-engineering scaffolds that mimic the extracellular environment of elastic tissues. We have developed a “double-hydrophobic” block ELP, GPG, inspired by non-uniform distribution of two different hydrophobic domains in natural elastin. GPG has a block sequence of (VGGVG)5-(VPGXG)25-(VGGVG)5 that self-assembles to form nanofibers in water. Functional derivatives of GPG with appended amino acid motifs can also form nanofibers, a display of the block sequence’s robust self-assembling properties. However, how the block

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Won, J. I., J. W. Ro, and H. Choi. "Phase behavior and physical properties of elastin-like polypeptide (ELP) block co-polypeptides: effects of drug delivery carriers." New Biotechnology 44 (October 2018): S108. http://dx.doi.org/10.1016/j.nbt.2018.05.1002.

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Pica, Andrea, and Giuseppe Graziano. "A Rationalization of the Effect That TMAO, Glycine, and Betaine Exert on the Collapse of Elastin-like Polypeptides." Life 12, no. 2 (2022): 140. http://dx.doi.org/10.3390/life12020140.

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Elastin-like polypeptides (ELPs) are soluble in water at low temperature, but, on increasing the temperature, they undergo a reversible and cooperative, coil-to-globule collapse transition. It has been shown that the addition to water of either trimethylamine N-oxide (TMAO), glycine, or betaine causes a significant decrease of T(collapse) in the case of a specific ELP. Traditional rationalizations of these phenomena do not work in the present case. We show that an alternative approach, grounded in the magnitude of the solvent-excluded volume effect and its temperature dependence (strictly link

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Goel, Ridhima, Deepak Gulwani, and Thoudam Debraj Singh. "Abstract 6893: Synthesis and characterization of IL-4R ligand mimic (AP1)-functionalized elastin-like polypeptide-based nanopolymers as selective delivery vehicles for paclitaxel in ovarian cancer." Cancer Research 85, no. 8_Supplement_1 (2025): 6893. https://doi.org/10.1158/1538-7445.am2025-6893.

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Abstract Introduction- Elastin-like polypeptides (ELPs) have emerged as promising nanocarriers for drug administration, owing to their advantageous properties, including biocompatibility and distinctive biophysical behavior. These polymers can be tailored to incorporate functional motifs that allow for accurate drug loading and targeted delivery. In cancer therapy, ELP-based nanocarriers have shown potential by increasing drug solubility, stability, and allowing for tumor-specific targeting. This study aims to develop and evaluate AP1-functionalized ELP-based nanopolymers as vehicles for the “

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Attia, Sara Aly, Anh Tan Truong, Alvin Phan та ін. "αB-Crystallin Peptide Fused with Elastin-like Polypeptide: Intracellular Activity in Retinal Pigment Epithelial Cells Challenged with Oxidative Stress". Antioxidants 12, № 10 (2023): 1817. http://dx.doi.org/10.3390/antiox12101817.

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Background: Oxidative stress-induced retinal degeneration is among the main contributing factors of serious ocular pathologies that can lead to irreversible blindness. αB-crystallin (cry) is an abundant component of the visual pathway in the vitreous humor, which modulates protein and cellular homeostasis. Within this protein exists a 20 amino acid fragment (mini-cry) with both chaperone and antiapoptotic activity. This study fuses this mini-cry peptide to two temperature-sensitive elastin-like polypeptides (ELP) with the goal of prolonging its activity in the retina. Methods: The biophysical

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Nelson, Derek W., Alexander Connor, Yu Shen, and Ryan J. Gilbert. "Construction of an Elastin-like Polypeptide Gene in a High Copy Number Plasmid Using a Modified Method of Recursive Directional Ligation." SynBio 2, no. 2 (2024): 174–89. http://dx.doi.org/10.3390/synbio2020010.

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Elastin-like polypeptides (ELPs) are popular biomaterials due to their reversible, temperature-dependent phase separation and their tunability, which is achievable by evolving procedures in recombinant technology. In particular, recursive direction ligation by plasmid reconstruction (PRe-RDL) is the predominant cloning technique used to generate ELPs of varying lengths. Pre-RDL provides precise control over the number of (VPGXG)n repeat units in an ELP due to the selection of type IIS restriction enzyme (REs) sites in the reconstructed pET expression plasmid, which is a low-to-medium copy numb

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Choi, Minji, Jiyoung Kwon, Ji-Hyun Jang, Duck-Su Kim, and Hyun-Jung Kim. "Enhancing the Biological Properties of Organic–Inorganic Hybrid Calcium Silicate Cements: An In Vitro Study." Journal of Functional Biomaterials 15, no. 11 (2024): 337. http://dx.doi.org/10.3390/jfb15110337.

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(1) Background: This study aimed to enhance the biological properties of hydraulic calcium silicate cements (HCSCs) by incorporating organic and inorganic components, specifically elastin-like polypeptides (ELPs) and bioactive glass (BAG). We focused on the effects of these composites on the viability, migration, and osteogenic differentiation of human periodontal ligament fibroblasts (hPDLFs). (2) Methods: Proroot MTA was supplemented with 1–5 wt% 63S BAG and 10 wt% ELP. The experimental groups contained various combinations of HSCS with ELP and BAG. Cell viability was assessed using an MTT a

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Bidwell, Gene L., Fakhri Mahdi, Qingmei Shao, et al. "A kidney-selective biopolymer for targeted drug delivery." American Journal of Physiology-Renal Physiology 312, no. 1 (2017): F54—F64. http://dx.doi.org/10.1152/ajprenal.00143.2016.

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Improving drug delivery to the kidney using renal-targeted therapeutics is a promising but underdeveloped area. We aimed to develop a kidney-targeting construct for renal-specific drug delivery. Elastin-like polypeptides (ELPs) are nonimmunogenic protein-based carriers that can stabilize attached small-molecule and peptide therapeutics. We modified ELP at its NH2-terminus with a cyclic, seven-amino acid kidney-targeting peptide (KTP) and at its COOH-terminus with a cysteine residue for tracer conjugation. Comparative in vivo pharmacokinetics and biodistribution in rat and swine models and in v

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Mahdi, Fakhri, Alejandro R. Chade, and Gene L. Bidwell. "Utilizing a Kidney-Targeting Peptide to Improve Renal Deposition of a Pro-Angiogenic Protein Biopolymer." Pharmaceutics 11, no. 10 (2019): 542. http://dx.doi.org/10.3390/pharmaceutics11100542.

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Elastin-like polypeptides (ELP) are versatile protein biopolymers used in drug delivery due to their modular nature, allowing fusion of therapeutics and targeting agents. We previously developed an ELP fusion with vascular endothelial growth factor (VEGF) and demonstrated its therapeutic efficacy in translational swine models of renovascular disease and chronic kidney disease. The goal of the current work was to refine renal targeting and reduce off-target tissue deposition of ELP–VEGF. The ELP–VEGF fusion protein was modified by adding a kidney-targeting peptide (KTP) to the N-terminus. All c

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Shamji, Mohammed F., Odelia Ghodsizadeh, Allan H. Friedman, William J. Richardson, Ashutosh Chilkoti, and Lori A. Setton. "Development of a thermally responsive peptide for sustained deliver of solyble TNF receptor II to attenuate inflammatory events associated with radiculopathys." Clinical & Investigative Medicine 30, no. 4 (2007): 94. http://dx.doi.org/10.25011/cim.v30i4.2873.

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Background: Tumor necrosis factor alpha (TNFα) is a cytokine that may mediate inflammatory histopathology of the dorsal root ganglion following lumbar disc herniation.1 Soluble TNF receptor II (sTNFRII) competitively binds TNFa with clinical value for painful radiculopathy.2 Bioactive peptides expressed with elastin-like polypeptides (ELP) fusion partners gain a thermally responsive domain, by which they can undergo hydrophobic collapse and separate from solution to aggregate at physiological temperatures.3 Protein release from such a depot may locally sustain drug presence, an effect demonstr

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Wada, Iori, Parameswaran G. Sreekumar, Christine Spee, Andrew J. MacKay, Michael Ip та Ram Kannan. "Mechanisms of Epithelial-Mesenchymal Transition and Prevention of Dispase-Induced PVR by Delivery of an Antioxidant αB Crystallin Peptide". Antioxidants 11, № 10 (2022): 2080. http://dx.doi.org/10.3390/antiox11102080.

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Proliferative Vitreoretinopathy (PVR) is a refractory retinal disease whose primary pathogenesis involves the epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells. At present, there is no effective treatment other than surgery for PVR. The purpose of this study was to investigate the effect of αB crystallin peptide (αBC-P) on EMT in PVR. We have previously shown that this peptide is antiapoptotic and regulates RPE redox status. Subconfluent primary human RPE (hRPE) cells were stimulated by TGFβ2 (10 ng/mL) with or without αBC-P (50 or 75 μg/mL) for 48 h and express

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Kadam, Shephali, Atham Ali, Mateusz Pospiech, et al. "Abstract 2363: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99-A192 ScFv-ELP nanoparticle treatment." Cancer Research 84, no. 6_Supplement (2024): 2363. http://dx.doi.org/10.1158/1538-7445.am2024-2363.

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Abstract CD99 is a transmembrane protein overexpressed in acute myeloid leukemia (AML), presenting a potential novel therapeutic target. Our previously developed anti-CD99-A192, comprising scFv and elastin-like polypeptides (ELPs), showed promising anti-leukemic activity by inducing apoptosis in AML cell lines and prolonging survival in AML xenograft models. Considering CD99's expression and role in T cell activation, we proposed that anti-CD99-A192 could have a dual function: targeting leukemic cells while activating T cells. Blood samples were obtained from 20 healthy donors, and peripheral

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Mills, Carolyn E., Zachary Michaud, and Bradley D. Olsen. "Elastin-like Polypeptide (ELP) Charge Influences Self-Assembly of ELP–mCherry Fusion Proteins." Biomacromolecules 19, no. 7 (2018): 2517–25. http://dx.doi.org/10.1021/acs.biomac.8b00147.

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Waller, Jamarius P., John Aaron Howell, Hali Peterson, Eric M. George, and Gene L. Bidwell. "Elastin-Like Polypeptide: VEGF-B Fusion Protein for Treatment of Preeclampsia." Hypertension 78, no. 6 (2021): 1888–901. http://dx.doi.org/10.1161/hypertensionaha.121.17713.

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Preeclampsia is characterized by the development of elevated blood pressure during the second and third trimesters of pregnancy that is accompanied by end organ dysfunction. The pathogenesis of preeclampsia is multifactorial but is commonly characterized by endothelial dysfunction and the overproduction of antiangiogenic factors, including the soluble VEGF (vascular endothelial growth factor) receptor sFlt-1 (soluble Fms-like tyrosine kinase receptor 1). Previously, administration of exogenous VEGF-A, bound to a carrier protein called ELP (elastin-like polypeptide), significantly reduced free

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Dragojevic, Sonja, Lindsay Turner, Pallabi Pal, Amol V. Janorkar, and Drazen Raucher. "Elastin-like Polypeptide Hydrogels for Tunable, Sustained Local Chemotherapy in Malignant Glioma." Pharmaceutics 14, no. 10 (2022): 2072. http://dx.doi.org/10.3390/pharmaceutics14102072.

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Glioblastoma (GBM) is a primary brain tumor that carries a dismal prognosis, which is primarily attributed to tumor recurrence after surgery and resistance to chemotherapy. Since the tumor recurrence appears near the site of surgical resection, a concept of immediate and local application of chemotherapeutic after initial tumor removal could lead to improved treatment outcome. With the ultimate goal of developing a locally-applied, injectable drug delivery vehicle for GBM treatment, we created elastin-like polypeptide (ELP) hydrogels. The ELP hydrogels can be engineered to release anti-cancer

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TURNER, PAUL A., GAURAV V. JOSHI, C. ANDREW WEEKS, R. SCOTT WILLIAMSON, AARON D. PUCKETT, and AMOL V. JANORKAR. "NANO AND MICRO-STRUCTURES OF ELASTIN-LIKE POLYPEPTIDE-BASED MATERIALS AND THEIR APPLICATIONS: RECENT DEVELOPMENTS." Nano LIFE 03, no. 04 (2013): 1343002. http://dx.doi.org/10.1142/s1793984413430022.

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Elastin-like polypeptide (ELP) containing materials have spurred significant research interest for biomedical applications exploiting their biocompatible, biodegradable and nonimmunogenic nature while maintaining precise control over their chemical structure and functionality through genetic engineering. Physical, mechanical and biological properties of ELPs could be further manipulated using genetic engineering or through conjugation with a variety of chemical moieties. These chemical and physical modifications also achieve interesting micro- and nanostructured ELP-based materials. Here, we r

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Huang, Kaizong, Mengyue Gao, Lin Fan, Yueyang Lai, Hongwei Fan, and Zichun Hua. "IR820 covalently linked with self-assembled polypeptide for photothermal therapy applications in cancer." Biomaterials Science 6, no. 11 (2018): 2925–31. http://dx.doi.org/10.1039/c8bm00399h.

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Elastin-like polypeptide covalently was linked with IR820 in an aqueous environment, followed by self-assembly into nanoparticles after adding to zinc ions solution. ELP-IR820 nanoparticles significantly accumulated at the tumor site.

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Cai, Lei, Cong B. Dinh, and Sarah C. Heilshorn. "One-pot synthesis of elastin-like polypeptide hydrogels with grafted VEGF-mimetic peptides." Biomater. Sci. 2, no. 5 (2014): 757–65. http://dx.doi.org/10.1039/c3bm60293a.

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We present a one-pot, single-step, cell-compatible strategy to tether VEGF-mimetic QK peptides into elastin-like polypeptide (ELP) hydrogels while simultaneously encapsulating endothelial cells to promote their three-dimensional outgrowth.

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Dragojevic, Sonja, Lindsay Turner, and Drazen Raucher. "Circumventing Doxorubicin Resistance Using Elastin-like Polypeptide Biopolymer-Mediated Drug Delivery." International Journal of Molecular Sciences 23, no. 4 (2022): 2301. http://dx.doi.org/10.3390/ijms23042301.

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Although doxorubicin (dox), an anthracycline antibiotic, is widely used and effective in treating cancer, its treatment efficiency is limited by low blood plasma solubility, poor pharmacokinetics, and adverse side effects, including irreversible cardiotoxicity. Moreover, cancer cells often develop drug resistance over time, which decreases the efficacy of anti-cancer drugs, including dox. In this study, we examine a macromolecular drug delivery system for its ability to specifically deliver doxorubicin to cancer cells with and without drug resistance. This drug delivery system consists of a mu

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Ingrole, Rohan S., Wenqian Tao, Jatindra N. Tripathy, and Harvinder S. Gill. "Synthesis and Immunogenicity Assessment of Elastin-Like Polypeptide-M2e Construct as an Influenza Antigen." Nano LIFE 04, no. 02 (2014): 1450004. http://dx.doi.org/10.1142/s1793984414500044.

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The 23 amino acid-long extracellular domain of the influenza virus transmembrane protein M2 (M2e) has remained highly conserved since the 1918 pandemic, and is thus considered a good candidate for development of a universal influenza A vaccine. However, M2e is poorly immunogenic. In this study we assessed the potential of increasing immunogenicity of M2e by constructing a nanoscale-designed protein polymer containing the M2e sequence and an elastin-like polypeptide (ELP) nanodomain consisting of alanine and tyrosine guest residues (ELP(A2YA2)24). The ELP nanodomain was included to increase ant

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Kim, Hyun-Jung, Donghyun Lee, Seungryong Cho, Ji-Hyun Jang, Sahng Gyoon Kim, and Sun-Young Kim. "Improvement of the Bonding Properties of Mineral Trioxide Aggregate by Elastin-Like Polypeptide Supplementation." Scanning 2019 (August 19, 2019): 1–8. http://dx.doi.org/10.1155/2019/3484396.

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Introduction. Elastin-like polypeptide (ELP) supplementation was previously reported to enhance the physical properties of mineral trioxide aggregate (MTA). The aim of this study was to investigate the effect of ELP supplementation on the bonding properties of MTA to dentin. Methods. Two types of ELPs were synthesized and mixed with MTA in a 0.3 liquid/powder ratio. The push-out bond strength test and interfacial observation with scanning electron microscopy were performed for ELP-supplemented MTA. The porosity of MTA fillings in the cavity was observed with microcomputed tomography. The stick

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Mills, Carolyn E., Erika Ding, and Bradley Olsen. "Protein Purification by Ethanol-Induced Phase Transitions of the Elastin-like Polypeptide (ELP)." Industrial & Engineering Chemistry Research 58, no. 27 (2019): 11698–709. http://dx.doi.org/10.1021/acs.iecr.9b00769.

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Ro, Jang-Won, Heelak Choi, Tae-Young Heo, Soo-Hyung Choi, and Jong-In Won. "Characterization of Amphiphilic Elastin-like Polypeptide (ELP) Block Copolymers as Drug Delivery Carriers." Biotechnology and Bioprocess Engineering 23, no. 6 (2018): 627–33. http://dx.doi.org/10.1007/s12257-018-0365-7.

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Sarangthem, Vijaya, Harshita Sharma, Mohini Mendiratta, et al. "Application of Bio-Active Elastin-like Polypeptide on Regulation of Human Mesenchymal Stem Cell Behavior." Biomedicines 10, no. 5 (2022): 1151. http://dx.doi.org/10.3390/biomedicines10051151.

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Regenerative medicine using stem cells offers promising strategies for treating a variety of degenerative diseases. Regulation of stem cell behavior and rejuvenate senescence are required for stem cells to be clinically effective. The extracellular matrix (ECM) components have a significant impact on the stem cell’s function and fate mimicking the local environment to maintain cells or generate a distinct phenotype. Here, human elastin-like polypeptide-based ECM-mimic biopolymer was designed by incorporating various cell-adhesion ligands, such as RGD and YIGSR. The significant effects of bioac

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Chen, Yingli, Yuanyuan Wu, Fengmin Xiong, et al. "Construction of a Collagen-like Protein Based on Elastin-like Polypeptide Fusion and Evaluation of Its Performance in Promoting Wound Healing." Molecules 28, no. 19 (2023): 6773. http://dx.doi.org/10.3390/molecules28196773.

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In the healing of wounds, human-like collagen (hCol) is essential. However, collagen-based composite dressings have poor stability in vivo, which severely limits their current therapeutic potential. Based on the above, we have developed a recombinant fusion protein named hCol-ELP, which consists of hCol and an elastin-like peptide (ELP). Then, we examined the physicochemical and biological properties of hCol-ELP. The results indicated that the stability of the hCol-ELP fusion protein exhibited a more compact and homogeneous lamellar microstructure along with collagen properties, it was found t

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Lindeboom, Tom, Binwu Zhao, George Jackson, Carol K. Hall, and Amparo Galindo. "On the liquid demixing of water + elastin-like polypeptide mixtures: bimodal re-entrant phase behaviour." Physical Chemistry Chemical Physics 23, no. 10 (2021): 5936–44. http://dx.doi.org/10.1039/d0cp05013j.

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Chu, Hun-Su, Kyung-Ho Lee, Ji-Eun Park, Dong-Myung Kim, Byung-Gee Kim, and Jong-In Won. "Expression analysis of an elastin-like polypeptide (ELP) in a cell-free protein synthesis system." Enzyme and Microbial Technology 46, no. 2 (2010): 87–91. http://dx.doi.org/10.1016/j.enzmictec.2009.10.003.

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Newman, Kristen, Kendra Clark, Bhuvaneswari Gurumurthy, Pallabi Pal, and Amol V. Janorkar. "Elastin-Collagen Based Hydrogels as Model Scaffolds to Induce Three-Dimensional Adipocyte Culture from Adipose Derived Stem Cells." Bioengineering 7, no. 3 (2020): 110. http://dx.doi.org/10.3390/bioengineering7030110.

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This study aimed to probe the effect of formulation of scaffolds prepared using collagen and elastin-like polypeptide (ELP) and their resulting physico-chemical and mechanical properties on the adipogenic differentiation of human adipose derived stem cells (hASCs). Six different ELP-collagen scaffolds were prepared by varying the collagen concentration (2 and 6 mg/mL), ELP addition (6 mg/mL), or crosslinking of the scaffolds. FTIR spectroscopy indicated secondary bonding interactions between collagen and ELP, while scanning electron microscopy revealed a porous structure for all scaffolds. Inc

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Sarangthem, Vijaya, Harshita Sharma, Ridhima Goel, et al. "Application of elastin-like polypeptide (ELP) containing extra-cellular matrix (ECM) binding ligands in regenerative medicine." International Journal of Biological Macromolecules 207 (May 2022): 443–53. http://dx.doi.org/10.1016/j.ijbiomac.2022.03.018.

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Yang, Jing, Yuanyu Zhang, Guojie Xing, et al. "Increased Accumulation of Recombinant Proteins in Soybean Seeds via the Combination Strategy of Polypeptide Fusion and Suppression of Endogenous Storage Proteins." Agronomy 13, no. 11 (2023): 2680. http://dx.doi.org/10.3390/agronomy13112680.

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Soybean seeds show great potential as a safe and cost-effective host for the large-scale production of biopharmaceuticals and industrially important macromolecules. However, the yields of desired recombinant proteins in soybean seeds are usually lower than the economic threshold for their potential commercialization. Our previous study demonstrated that polypeptide fusion such as maize γ-zein or elastin-like polypeptide (ELP) could significantly increase the accumulation of foreign proteins. In the present study, a recombination strategy of polypeptide fusions (γ-zein or ELP) and suppression o

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Kallon, Juliet, John J. Bang, Ufana Riaz, and Darlene K. Taylor. "Design of Thermo-Responsive Pervaporation Membrane Based on Hyperbranched Polyglycerols and Elastin-like Protein Conjugates." Nanomaterials 14, no. 22 (2024): 1821. http://dx.doi.org/10.3390/nano14221821.

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This paper reports the development of a highly crosslinked hyper-branched polyglycerol (HPG) polymer bound to elastin-like proteins (ELPs) to create a membrane that undergoes a distinct closed-to-open permeation transition at 32 °C. The crosslinked HPG forms a robust, mesoporous structure (150–300 nm pores), suitable for selective filtration. The membranes were characterized by FTIR, UV–visible spectroscopy, SEM, and AFM, revealing their structural and morphological properties. Incorporating a synthetic polypeptide introduced thermo-responsive behavior, with the membrane transitioning from imp

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Widder, Katharina, Sarah R. MacEwan, Elisabeth Garanger, et al. "Characterisation of hydration and nanophase separation during the temperature response in hydrophobic/hydrophilic elastin-like polypeptide (ELP) diblock copolymers." Soft Matter 13, no. 9 (2017): 1816–22. http://dx.doi.org/10.1039/c6sm02427k.

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Chowdhury, Sheetal, Joshua S. Speed, Gene L. Bidwell, and Amol V. Janorkar. "Influence of RGD in Elastin-Based Coatings on Morphology and Differentiation of Three-Dimensional Adipocyte Spheroids." Coatings 15, no. 7 (2025): 763. https://doi.org/10.3390/coatings15070763.

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Three-dimensional (3D) in vitro adipocyte models provide physiologically relevant platforms for studying adipogenesis and obesity-related metabolic dysfunction. However, long-term adipocyte culture is often hindered by limited cell–matrix adhesion and spheroid detachment. Previously, we demonstrated that elastin-like polypeptide (ELP)–polyethyleneimine (PEI) coatings functionalized with a trivalent RGD motif enhanced spheroid retention during frequent media changes. The present study investigates the long-term functional consequences of RGD incorporation over a 28-day culture period. 3T3-L1 pr

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Wu, Zhi, Huipeng Lu, Dewei Zhu, et al. "Developing an Indirect ELISA for the Detection of African Swine Fever Virus Antibodies Using a Tag-Free p15 Protein Antigen." Viruses 15, no. 9 (2023): 1939. http://dx.doi.org/10.3390/v15091939.

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African swine fever (ASF) is one of the most severe diseases caused by the ASF virus (ASFV), causing massive economic losses to the global pig industry. Serological tests are important in ASF epidemiological surveillance, and more antigen targets are needed to meet market demand for ASFV antibody detection. In the present study, ASFV p15 protein was fusion-expressed in Escherichia coli (E. coli) with elastin-like polypeptide (ELP), and the ELP-p15 protein was purified using a simple inverse transition cycling (ITC) process. The ELP tag was cleaved off using tobacco etch virus protease (TEVp),

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Tran, Thu Hoai, Hoang Ha Chu, Thi Thuong Ho, and Bich Ngoc Pham. "Optimization of extraction and purification of the M protein fused GCN4pII motif and elastin like polypeptide of porcine epidemic diarrhea virus from <i>Nicotiana benthamiana</i>." Vietnam Journal of Biotechnology 22, no. 4 (2024): 641–54. https://doi.org/10.15625/vjbt-21878.

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Porcine Epidemic Diarrhea (PED) has significantly impacted the swine farming industry in several countries worldwide, including Vietnam. Porcine Epidemic Diarrhea Virus (PEDV) has been proven to be the cause of PED. The COE and M proteins are potential candidates for subunit vaccine research against PEDV. The M protein, a largest envelope protein of PEDV, is highly conserved and plays a crucial role in virus assembly as well as in inducing the production of virus-neutralizing antibodies in the presence of complement. In a previous study, we constructed and expressed the M protein fused with th

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Liang, P., W. Zhuoran, G. Weiping, and W. Guihuai. "P12.01 Local implantation of thermoresponsive interferon alpha-polypeptide conjugate combined with temozolomide for post-surgical glioblastoma chemoimmunotherapy." Neuro-Oncology 21, Supplement_3 (2019): iii59. http://dx.doi.org/10.1093/neuonc/noz126.212.

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Abstract BACKGROUND Glioblastoma(GBM) is associated with gloomy prognosis despite maximal safe resection and following chemoradiation. In recent years, significant progress has been made in cancer immunotherapy except for GBM, which is largerly due to its local immunosuppressive microenvironment. Some research has demonstrated that local immunotherapy may be more efficient than systematic administrators. IFNα has been investigated as antitumor agent for some cancers, including glioma. Nevertheless, the short circulating half-life greatly limits its clinical application. Hence, a thermoresponsi

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