Bibliographies thématiques / EcoRI

Littérature scientifique sur le sujet « EcoRI »

Auteur : Grafiati
Publié le 10 mars 2023

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Articles de revues sur le sujet "EcoRI"

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LUKE, PETER A., et STEPHEN E. HALFORD. « Fluorescent labelling of EcoRI and EcoRV ». Biochemical Society Transactions 14, no 2 (1 avril 1986) : 259–60. http://dx.doi.org/10.1042/bst0140259a.

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Nascimento, Elmiro R., Al J. DaMassa, Richard Yamamoto et M. Graça F. Nascimento. « Plasmids in Mycoplasma species isolated from goats and sheep and their preliminary typing ». Revista de Microbiologia 30, no 1 (1999) : 32–36. http://dx.doi.org/10.1590/s0001-37141999000100006.

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One-hundred-five (105) clinical isolates of mycoplasma from caprine origin and one isolate from ovine were surveyed for plasmids, which were present in thirty-three (31%) of them. These mycoplasmas originated from 13 herds. Ten of them were symptomatic for mycoplasmal disease (mastitis, polyarthritis, septicemia) and three herds were asymptomatic, i.e., clinically normal. Twenty-eight isolates were Mycoplasma mycoides subspecies mycoides LC (large colony or caprine biotype), four were Mycoplasma capricolum subsp. capricolum and one was Mycoplasma cottewii. The isolated plasmids were linearized by EcoRI, EcoRV, EcoRI and EcoRV or BamHI and EcoRV, and were of five sizes (1.1, 1.6, 1.7, 1.8, and 1.9 Kbp). Based on restriction enzyme digestion and size of the linearized supercoiled extrachromosomal DNA, five plasmid types were recovered (p1II, p2III, p2V, p3I, and p4IV). The small size of these DNA elements probably exclude replicative forms of DNA virus, which are equal or larger than 8.0 Kbp.
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KENNETT, CHARLES A., et BENJAMIN STARK. « Automated Ribotyping for the Identification and Characterization of Foodborne Clostridia ». Journal of Food Protection 69, no 12 (1 décembre 2006) : 2970–75. http://dx.doi.org/10.4315/0362-028x-69.12.2970.

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The DuPont Qualicon RiboPrinter was employed to determine if automated ribotyping could be used to differentiate between and characterize various species of foodborne clostridia. EcoRI digests were used to ribotype 49 isolates that represented seven Clostridium species: C. aerotolerans, C. beirjerinckii, C. botulinum, C. butyricum, C. perfringens, C. putrificum, and C. sporogenes. EcoRV digests were also used to ribotype 17 C. botulinum isolates to determine if an alternate restriction enzyme was more suitable than was EcoRI for toxin typing. It was concluded that the RiboPrinter could be potentially used to identify most of the clostridia represented in the study, but that the system has difficulty distinguishing between C. botulinum and C. sporogenes. The system may also be potentially used to characterize clostridia based on phenotypic characteristics. Toxin typing of clostridia remains problematic, but may be improved by the use of restriction enzyme combinations.
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del Arco, A., et M. Izquierdo. « De novo methylation causes a tissue-specific polymorphic EcoRI pattern at the human epidermal growth factor receptor gene ». Biochemical Journal 292, no 2 (1 juin 1993) : 591–95. http://dx.doi.org/10.1042/bj2920591.

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A novel restriction polymorphism, probably due to tissue-specific methylation, has been identified at the human epidermal-growth-factor-receptor (EGF-R) gene. DNA isolated from smooth muscle showed altered EcoRI restriction bands when hybridized with different fragments of the EGF-R cDNA. These bands were absent in brain or leucocyte DNA samples from the same individuals. Three restriction sites, partly resistant to cleavage by EcoRI, were characterized in muscle DNA which were not clustered but instead were scattered along the gene. The flanking sequences of one of these resistant EcoRI sites were determined. This specific EcoRI site was followed by a 3′-guanosine generating a methylatable EcoRI sequence. This suggests that the failure to digest to completion these EcoRI sites was due to modification by methylation. In addition, we noted that EcoRI sites were affected at both alleles, indicating that de novo methylation changes, and not methylation events related to genomic imprinting, would cause the muscle-specific EcoRI pattern. Also abnormal restriction fragments with XbaI were observed in muscle DNA. A large number of unrelated muscle DNA samples have been analysed, and all of them displayed an identical EcoRI polymorphic pattern, suggesting that DNA modification by de novo methylation events could be functionally relevant.
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Kodogo, Vitaris, Danai Tavonga Zhou, Olav Oektedalen, Kerina Duri, Babill Stray-Pedersen et Exnevia Gomo. « Apolipoprotein B Gene Polymorphisms and Dyslipidemia in HIV Infected Adult Zimbabweans ». Open AIDS Journal 10, no 1 (30 septembre 2016) : 190–98. http://dx.doi.org/10.2174/1874613601610010190.

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Background:Dyslipidemia does not occur in all HIV-infected or antiretroviral therapy-experienced patients suggesting role of host genetic factors but there is paucity of data on association between dyslipidemia and gene polymorphisms in Zimbabwe.Objective:To determine association of lipoprotein levels andapolipoprotein Bpolymorphisms in HIV-infected adults.Method:Demographic data were collected from 103 consenting patients; lipoprotein levels were determined and blood samples were successfully genotyped for bothapolipoprotein B2488C>T Xba1 andapolipoprotein B4154G>A p.Gln4154Lys EcoR1 polymorphisms by real time polymerase chain reaction.Results:Mean age of genotyped patients was 40.3 ± 10.1 years, 68% were female; prevalence of dyslipidemia was 67.4%. Of 103 samples genotyped forapolipoprotein BXba1 polymorphism, 76 (74%) were homozygous C/C, 24 (23%) were heterozygous C/T and only three (3%) were homozygous T/T.Apolipoprotein BEcoR1 polymorphism showed little variability, one participant had rare genotype A/A, 68.3% had wild type genotype G/G.Conclusion:Observed frequencies ofapolipoprotein BXbaI and EcoRI polymorphisms matched other African studies. In spite of low numbers of rare variants, there was positive association between both total cholestrol and high density lipoprotein with ECoR1 wild type G/G genotype, suggesting that ECoRI 4154 G allele could be more protective against coronary heart disease than EcoR1 4154 A allele. There is need for further research at population level to confirm whetherapolipoprotein BECoR1 genotyping is useful for predicting risk of dyslipidemia in HIV patients in our setting.
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Chen, Jianzhu, Leonard A. Herzenberg et Leonore A. Herzenberg. « Heparin inhibits EcoRI endonuclease cleavage of DNA at certain EcoRI sites ». Nucleic Acids Research 18, no 11 (1990) : 3255. http://dx.doi.org/10.1093/nar/18.11.3255.

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Song, Yonghai, Dan Luo, Shuhong Ye, Mei Huang, Dandan Zhong, Zhenzhong Huang, Haoqing Hou et Li Wang. « Spectroscopic studies on the interaction between EcoRI and CdS QDs and conformation of EcoRI in EcoRI-CdS QDs bioconjugates ». Physical Chemistry Chemical Physics 14, no 47 (2012) : 16258. http://dx.doi.org/10.1039/c2cp42562a.

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Liu, Yaoping, Asao Ichige et Ichizo Kobayashi. « Regulation of the EcoRI restriction–modification system : Identification of ecoRIM gene promoters and their upstream negative regulators in the ecoRIR gene ». Gene 400, no 1-2 (octobre 2007) : 140–49. http://dx.doi.org/10.1016/j.gene.2007.06.006.

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Terry, B. J., W. E. Jack et P. Modrich. « Facilitated diffusion during catalysis by EcoRI endonuclease. Nonspecific interactions in EcoRI catalysis. » Journal of Biological Chemistry 260, no 24 (octobre 1985) : 13130–37. http://dx.doi.org/10.1016/s0021-9258(17)38848-8.

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OLD, D. C., S. A. CHISHOLM, P. B. CRICHTON et A. TAYLOR. « Grouping of Salmonella enterica serotype Montevideo strains by ribotyping and IS200 profiling ». Epidemiology and Infection 124, no 3 (juin 2000) : 375–82. http://dx.doi.org/10.1017/s0950268899004033.

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One-hundred and twenty-one isolates of Salmonella enterica serotype Montevideo, representing different biotypes and incidents of infection detected in the UK between 1977 and 1995, were analysed by EcoRI ribotyping, PvuII ribotyping and IS200 fingerprinting. Among the isolates examined, 7 EcoRI ribotypes, 5 PvuII ribotypes and 55 IS200 profile types were recognized and 4 arbitrary groups defined. All 33 isolates of biotype 2d belonged to EcoRI/PvuII ribotype 1/1 and IS200 lineage A and comprised Group I. The other 88 isolates of biotype 10di and its variants were assigned to Groups II–IV. All 27 isolates in Group II were of EcoRI/PvuII ribotype 2/2 and IS200 lineage B. Among the 43 isolates in Group III, 42 of which were of EcoRI/PvuII ribotype 3/3, IS200 analysis identified 38 profiles in lineages C–I. Six EcoRI/PvuII ribotypes and 8 IS200 profiles, mostly in lineages C–E, were recognized among the 18 isolates in Group IV. The combined use of biotyping and ribotyping, and to some extent IS200 profiling, has enhanced our understanding of the clonal structure of serotype Montevideo and provides a basis for further study.
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Thèses sur le sujet "EcoRI"

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Luke, P. A. « The EcoRI and EcoRV restriction endonucleases ». Thesis, University of Bristol, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372027.

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Vennekohl, Petra. « Bedeutung der Dimerisierung für Spezifität und Katalyse der Restriktionsendonuklease EcoRI ». [S.l. : s.n.], 1999. http://deposit.ddb.de/cgi-bin/dokserv?idn=959597549.

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Peters, Imke. « Veränderung der Sequenzspezifität der Restriktionsendonuklease EcoRI ». [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=971220905.

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Küster, Wolfgang. « Bedeutung hydrophober Kontakte für die sequenzspezifische DNA-Erkennung der Restriktionsendonuklease EcoRI ». [S.l. : s.n.], 1998. http://deposit.ddb.de/cgi-bin/dokserv?idn=955012392.

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Rosati, Olaf. « Untersuchung und Design von DNA-Kontakten der Restriktionsendonuklease EcoRI inner- und ausserhalb der Erkennungssequenz ». [S.l. : s.n.], 1999. http://deposit.ddb.de/cgi-bin/dokserv?idn=958768870.

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Chan, Yee-man, et 陳綺雯. « Transcriptional regulation in the EcoRI-F immunity region of the Bacillus subtilis phage [phi] 105 ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B29474528.

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Hatcher, Mary Elana. « A solid-state deuterium NMR investigation of the local dynamics of nucleotides in the EcoRI restriction endonuclease binding site / ». Thesis, Connect to this title online ; UW restricted, 1996. http://hdl.handle.net/1773/8640.

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Bougueleret, Lydie. « Contribution à l'étude des systèmes de restriction modification EcoR I et EcoR V ». Paris 7, 1985. http://www.theses.fr/1985PA077010.

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Les gènes codant pour le système de restriction modification EcoR I ont été clonés séparément dans les plasmides vecteurs pBR322 et pACYC184. L'étude de mutants spontanés de l'endonucléase EcoR I mec en évidence le caractère létal eu gène de l'endonucléase. De plus ce gène apparaît être un site préférentiel d'intégration pour l'élément d'insertion IS1. Un plasmide codant pour le système de restriction modification EcoR V a été isolé et caractérisé. Ce plasmide, pLB1, a une taille de 6,2 Kb et porte comme seuls marqueurs identifiables le système EcoR V. Les deux gènes codant pour l'endonucléase et la méthylase ont été localisés sur un fragment 3 Kb sous-cloné dans pBR322. Les positions relatives de ces deux gènes ont été déterminées par construction d'une série de délétions chevauchantes. La séquence nucléotidique du segment de 2,2 Kb contenant toute l'information génétique nécessaire à l'expression du système a été déterminée. Les deux gènes sont transcrits en direction opposée à partir d'une région intergénique de 310 pdb. L'identification des régions codantes a été confirmée par la séquence des protéines. La structure secondaire potentielle de l’ARN messager permet de proposer un modèle de modulation de la traduction du gène de l'endonucléase. D'autre part nous avons construit des clones surproduisant l'endonucléase et la méthylase en plaçant cep deux gènes sous le contrôle du promoteur PL de lambda.
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Grabowski, Gabriele. « Untersuchungen zum Mechanismus der Katalyse und zu In-vivo-Selektionssystemen für die Anreicherung von Mutanten der Restriktionsendonuklease EcoRI mit veränderter DNA-Bindungs- oder Spaltspezifität ». [S.l. : s.n.], 1998. http://deposit.ddb.de/cgi-bin/dokserv?idn=954823028.

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Schubert, Susanne, Sandra Heller, Birgit Löffler, Ingo Schäfer, Martina Seibel, Gaetano Villani et Peter Seibel. « Generation of rho zero cells ». Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-167888.

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Human mitochondrial DNA (mtDNA) is located in discrete DNA-protein complexes, so called nucleoids. These structures can be easily visualized in living cells by utilizing the fluorescent stain PicoGreen®. In contrary, cells devoid of endogenous mitochondrial genomes (ρ0 cells) display no mitochondrial staining in the cytoplasm. A modified restriction enzyme can be targeted to mitochondria to cleave the mtDNA molecules in more than two fragments, thereby activating endogenous nucleases. By applying this novel enzymatic approach to generate mtDNA-depleted cells the destruction of mitochondrial nucleoids in cultured cells could be detected in a time course. It is clear from these experiments that mtDNA-depleted cells can be seen as early as 48 h post-transfection using the depletion system. To prove that mtDNA is degraded during this process, mtDNA of transfected cells was quantified by real-time PCR. A significant decline could be observed 24 h post-transfection. Combination of both results showed that mtDNA of transfected cells is completely degraded and, therefore, ρ0 cells were generated within 48 h. Thus, the application of a mitochondrially-targeted restriction endonuclease proves to be a first and fast, but essential step towards a therapy for mtDNA disorders.
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Livres sur le sujet "EcoRI"

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ECORS-Pirineos, Proyecto Hispano-Francés. Proyecto Hispano-Francés ECORS-Pirineos. Madrid : Repsol, Exploración, 1992.

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Popescu-Gogan, Petre. Ecouri eminesciene în arta plastică. [Bucharest] : Editura Meridiane, 1992.

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Ecouri din holocaust în literatura universală. București : Editura Hasefer, 2003.

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François, Roure, dir. The ECORS-CROP Alpine seismic traverse. Paris : Société géologique de France, 1996.

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Madan, Gheorghe. Grădina cu cireșe coapte : Ecouri istorice. Chișinău : Știința, 2001.

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Quiñones, Tato. Ecorie Abakuá : Cuatro ensayos sobre los ñáñigos cubanos. [La Habana] : Ediciones Unión, 1994.

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Șotropa, Adriana. Visuri și himere : Ecouri simboliste în sculptura românească modernă. București : Compania, 2009.

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International Committee of the Red Cross. Archives et Schweizerisches Bundesarchiv, dir. Ecouri dintr-o epocă tulbure : Documente elvețiene, 1940-1944. București : Editura Hasefer, 1998.

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ECORS, Programme. Deep seismic study of the earth's crust : ECORS Bay of Biscay survey = Etude de la croûte terrestre par sismique profonde : Campagne ECORS-Gascogne. Paris : Société géologique de France, 1997.

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"Un palat de ecouri" : Literatura din perspectivă semiotică, stilistică, imagologică. Craiova : Universitaria, 2020.

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Chapitres de livres sur le sujet "EcoRI"

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Jen-Jacobson, L., D. R. Lesser et M. R. Kurpiewski. « DNA Sequence Discrimination by EcoRI Endonuclease ». Dans Nucleic Acids and Molecular Biology, 141–70. Berlin, Heidelberg : Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-84292-4_10.

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Segall, M., L. Schluender, H. Betuel et M. Garovoy. « RFLP Standardization Report for C4/EcoRI ». Dans Immunobiology of HLA, 765–67. New York, NY : Springer New York, 1989. http://dx.doi.org/10.1007/978-1-4612-3552-1_205.

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Segall, M., L. Schluender, H. Betuel et M. Garovoy. « RFLP Standardization Report for 210H/EcoRI ». Dans Immunobiology of HLA, 782–84. New York, NY : Springer New York, 1989. http://dx.doi.org/10.1007/978-1-4612-3552-1_216.

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Segall, M., L. Schluender, H. Betuel et M. Garovoy. « RFLP Standardization Report for BF/EcoRI ». Dans Immunobiology of HLA, 730. New York, NY : Springer New York, 1989. http://dx.doi.org/10.1007/978-1-4612-3552-1_183.

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Segall, M., L. Schluender, H. Betuel et M. Garovoy. « RFLP Standardization Report for C2/EcoRI ». Dans Immunobiology of HLA, 749–50. New York, NY : Springer New York, 1989. http://dx.doi.org/10.1007/978-1-4612-3552-1_195.

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Segall, M., L. Schluender, B. Bradley et S. Naito. « RFLP Standardization Report for DR Alpha/EcoRI ». Dans Immunobiology of HLA, 801–3. New York, NY : Springer New York, 1989. http://dx.doi.org/10.1007/978-1-4612-3552-1_227.

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Segall, M., L. Schluender, H. Beteul, B. Bradley, M. Garovoy et S. Naito. « RFLP Standardization Report for DQ Alpha/EcoRI ». Dans Immunobiology of HLA, 822–23. New York, NY : Springer New York, 1989. http://dx.doi.org/10.1007/978-1-4612-3552-1_237.

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Segall, M., L. Schluender, B. Bradley et S. Naito. « RFLP Standardization Report for DP Alpha/EcoRI ». Dans Immunobiology of HLA, 842–43. New York, NY : Springer New York, 1989. http://dx.doi.org/10.1007/978-1-4612-3552-1_248.

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Tonks, S., V. Groves, H. Betuel, J. L. Bidwell, M. L. Garavoy, R. Lechler, S. Naito, J. Bodmer et J. Trowsdale. « RFLP Standardization Report for DR Beta/EcoRI ». Dans Immunobiology of HLA, 596–98. New York, NY : Springer New York, 1989. http://dx.doi.org/10.1007/978-1-4612-3552-1_126.

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Tonks, S., V. Groves, H. Betuel, J. L. Bidwell, M. L. Garavoy, R. Lechler, S. Naito, J. Bodmer et J. Trowsdale. « RFLP Standardization Report for DQ Beta/EcoRI ». Dans Immunobiology of HLA, 627–29. New York, NY : Springer New York, 1989. http://dx.doi.org/10.1007/978-1-4612-3552-1_138.

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Actes de conférences sur le sujet "EcoRI"

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Matthews, R. J., I. R. Peake et A. L. Bloom. « POINT-MUTATION OF FACTOR VIII CODING SEQUENCES IN HAEMOPHILIA A ». Dans XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644013.

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In order to study the molecular basis of haemophilia A, DNA from 26 haemophilia A patients (8 severe with inhibitors, 13 severe noninhibitors and 5 mild/moderate) was screened by the Southern blotting method with FVIII cDNA probe A (a i.7kb Kpnl cDNA fragment that spans exons 1 to 12) probe B (a 4.7kb EcoRI cDNA fragment that contains exons 14 to 25 and part of exon 26) probe C (a 1.8kb EcoRI cDNA fragment that contains the remainder of exon 26 and probe D (an Apal/EcoRI 783bp cDNA fragment that includes all of exons 22 to 25 and parts of exons 21 and 26). All cDNA probes were kindly provided by Genetics Institutes Inc.No large structural alterations of the FVIII gene were detected in any of the patients. However altered TaqI restriction sites within the coding regions of 3 patients were observed. DNA from patient 1 with severe haemophilia (VIIIAg < 0.1 u/dl, inhibitor negative) when probed with probe C showed a substitution of the normal 2.6kb TaqI and 4.5kb EcoRI fragments with novel 12kb TaqI and 11.5kb EcoRI fragments respectively. In addition he showed the normal 4kb Bglll fragment with probe C. A point mutation or small deletion (50bp is suspected to be present within exon 26.Patient 2 had severe haemophilia and a FVIII inhibitor of 12 units (Bethesda). DNA from patient 2 when probed with probe B revealed a novel 5.0kb TaqI fragment instead of the normal 2.2kb and 2.8kb fragments.The location of the altered Taq I restriction site within the coding region of exon 18 was confirmed with intragenomic probe pi 14.12 that includes exons 17and 18 (kindly provided by Genentech Inc.) A family study with this mutation specific fragment showed the patients sister and mother to be carriers.DNA from Patient 3 (severe haemophilia, factor VIII inhibitor 33 units) when probed independently with probes B and D revealed the absence of the normal 2.4kb and 1.4kb TaqI fragments and the generation of a novel 3.8kb TaqI fragment suggesting an alteration of the TaqI site within the coding region of exon 23.The detection of altered TaqI restriction sites in 3of our patients is further evidence that 'CG' dinucleotide sequences might be relative hot-spots for mutation when occurring within coding sequences of genes.
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Chelucci, C., H. J. Hassan, R. Guerriero, A. Leonardi, G. Mattia et C. Peschle. « POLYMORPHIC SITES IN FACTOR X GENE ». Dans XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643836.

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The structure of factor X gene has been analyzed by Southern blot in 5 subjects with factor X deficiency.Genomic DNA was digested with 8 different endonucleases and hybridized with a cDNA probe. The congenital deficiency observed in these patients is not apparently due to a major deletion or rearrangement. Since the gene locus is grossly intact, the disease presumably results from point mutation(s) not identified by the utilized endonucleases.Our study was also focused on the presence of polymorphic site(s) in the factor X gene locus. Analysis of 50 normal subjects allowed to identify several polymorphic restriction sites after digestion with EcoRI, Hind III and Pvu II.The restriction pattern obtained after Hind III digestion showed two bands of 7.3 and 6.0 Kb, while in two families an additional 7.6 Kb band was observed. Genomic DNA digested with EcoRI showed a 7.1 and 5.1 Kb fragments, and also a 6.6 Kb band with a 10% f requency.After DNA digestion with Pvu II 5.6, 2.7 and ∼1.0 Kb bands were observed. In three unrelated subjects we observed an additional 3.0 Kb fragment, in two other subjects a 3.5 kb band. Interestingly hybridization with a 178 bp cDNA subclone allowed to map the polymorphic sites in a the 3’ region of the gene locus.
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Казанцева, О. А., М. О. Нагорных et М. В. Захарова. « Новый конвергентный промотор As3 системы рестрикции-модификации II типа EcoRI играет ключевую роль в регуляции экспрессии гена эндонуклеазы рестрикции ». Dans VI Пущинская школа-конференция «Биохимия, физиология и биосферная роль микроорганизмов». ИД «Вода : химия и экология», 2019. http://dx.doi.org/10.18334/ibpm2019.111-113.

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Казанцева, О. А., М. О. Нагорных et М. В. Захарова. « Новый конвергентный промотор As3 системы рестрикции-модификации II типа EcoRI играет ключевую роль в регуляции экспрессии гена эндонуклеазы рестрикции ». Dans VI Пущинская школа-конференция «Биохимия, физиология и биосферная роль микроорганизмов». ИД «Вода : химия и экология», 2019. http://dx.doi.org/10.18334/ibpm2019_111-113.

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Spitzer, S. G., P. Usharani, A. D. Roser, C. K. Kasper et S. G. Bajaj. « THE CATALYTIC TRIAD RESIDUES (HIS221, ASP269, SER365) AND THE BINDING POCKET RESIDUE (ASP359) IN FACTOR IXBm ELSINORE (IXBmLE) ARE NOT ALTERED ». Dans XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644071.

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Previous studies suggested that the defect in IXBmLE (a nonfunctional variant of human IX) is either in the catalytic triad or at the site(s) of interaction with the macromolecular substrates (antithrombin III, factor VII or factor X). To distinguish between these possibilities, we isolated a complete IX cDNA clone from a human liver cDNA library. We also constructed a genomic library (in phage EMBL3) using DNA of the BmLE patient. The library was screened with normal IX cDNA and with synthetic oligonucleotides. The positive clones containing the exons coding for IX were plaque purified. Two clones which contained the coding sequence of the catalytic domain, i.e., His221 (exon VII), and Asp269, Asp359, and Ser365 (exon VIII) were selected for further studies. The phage containing exon VIII was first digested with Sail and EcoRI and a 2-Kb fragment, which hybridized with the segment of cDNA containing exon VIII, was gel purified. The 2-Kb fragment was further digested and the subfragments were cloned into M13; the length and direction of the fragments used in sequencing are shown below:The phage containing exon VII was digested with PstI and SalI, and a 1-Kb fragment that hybridized with the 19-mer His221 probe was subcloned into M13 phage for sequencing. The sequence starting with residue Vall96 through residue Arg403 was found to be normal. Thus, none of the residues in the catalytic domain of IXBmLE are different from that of normal IX. These data provide strong indirect evidence that the noncatalytic aminoterminal portion of IX plays a significant role in the structural recognition of the macromolecular substrates. The sequence of this region of IXBmLE should provide information about the putative residue(s) essential for this recognition.
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Bailey, Everton. « ECOLI on Mote ». Dans SoutheastCon 2018. IEEE, 2018. http://dx.doi.org/10.1109/secon.2018.8478974.

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Perera Aracil, Jesús M., et Diego Sevilla Ruiz. « Towards Distributed Ecore Models ». Dans 4th International Conference on Model-Driven Engineering and Software Development. SCITEPRESS - Science and and Technology Publications, 2016. http://dx.doi.org/10.5220/0005685002090216.

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Rodri´guez, Marti´n A., Ricardo M. Carranza, Marshall L. Stuart et Raul B. Rebak. « Long-Term Corrosion Potential Behavior of Alloy 22 in Hot 5 m CaCl2 + 5 m Ca(NO3)2 Brines ». Dans ASME 2007 Pressure Vessels and Piping Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/pvp2007-26162.

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Alloy 22 is a nickel base alloy highly resistant to all forms of corrosion. In very aggressive conditions (e.g. hot concentrated chloride containing brines) Alloy 22 could suffer localized attack, namely pitting and crevice corrosion. The occurrence of localized corrosion in a given environment is governed by the values of the critical potential (Ecrit) for crevice corrosion and the corrosion potential (Ecorr) that the alloy may establish in the studied environment. If Ecorr is equal or higher than Ecrit, localized corrosion may be expected. This paper discusses the evolution of Ecorr of Alloy 22 specimens in 5 m CaCl2 + 5 m Ca(NO3)2 brines at 100°C and 120°C. Two types of specimens were used, polished as-welded (ASW) creviced and non-creviced specimens and as-welded plus solution heat-treated (ASW+SHT) creviced specimens. The latter contained the black annealing oxide film on the surface. Results show that, for all types of Alloy 22 specimens the Ecorr was higher at 120°C than at 100°C, probably because a more protective film formed at the higher temperature. Specimens with the black oxide film on the surface showed more oscillations in the potential. None of the tested specimens suffered crevice corrosion probably because of the relatively high concentration of nitrate in the electrolyte, R = [NO3]/[Cl] = 1.
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Babur, Önder. « Clone Detection for Ecore Metamodels using N-grams ». Dans 6th International Conference on Model-Driven Engineering and Software Development. SCITEPRESS - Science and Technology Publications, 2018. http://dx.doi.org/10.5220/0006604604110419.

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Debaque, Freedman, Goutal, Keller, Levy et Saba. « The ECORP approach to Petri net tool evaluation ». Dans Proceedings of Canadian Conference on Electrical and Computer Engineering CCECE-94. IEEE, 1994. http://dx.doi.org/10.1109/ccece.1994.405876.

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Rapports d'organisations sur le sujet "EcoRI"

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Feigon, Juli. Recognition of DNA by EcoRI Restriction Endonuclease and Methylase. Fort Belvoir, VA : Defense Technical Information Center, janvier 1992. http://dx.doi.org/10.21236/ada247626.

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Cook, David R. Eddy Correlation Flux Measurement System (ECOR) Instrument Handbook. Office of Scientific and Technical Information (OSTI), août 2018. http://dx.doi.org/10.2172/1467448.

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