Littérature scientifique sur le sujet « DNA pairing statistic »

Understanding of the pairing statistics in solutions populated by a large number of distinct solute species with mutual interactions is a challenging topic, relevant in modeling the complexity of real biological systems. Here we describe, both experimentally and theoretically, the formation of duplexes in a solution of random-sequence DNA (rsDNA) oligomers of length L = 8, 12, 20 nucleotides. rsDNA solutions are formed by 4L distinct molecular species, leading to a variety of pairing motifs that depend on sequence complementarity and range from strongly bound, fully paired defectless helices to weakly interacting mismatched duplexes. Experiments and theory coherently combine revealing a hybridization statistics characterized by a prevalence of partially defected duplexes, with a distribution of type and number of pairing errors that depends on temperature. We find that despite the enormous multitude of inter-strand interactions, defectless duplexes are formed, involving a fraction up to 15% of the rsDNA chains at the lowest temperatures. Experiments and theory are limited here to equilibrium conditions.

Other than being a carrier of the code of life deoxyribonucleic acid (DNA) can also be used as a kind of ideal biomaterial with good biocompatibility. The basis of DNA dynamic nanotechnology is the toehold-mediated strand displacement reactions. Utilizing the specificity and predictability of Watson–Crick base pairing, and the programmability of the base sequence of DNA, researchers can construct the molecular machine and precisely control its operation to realize various complex molecular calculations. In this paper, we reviewed the progress of DNA structure and mechanical properties in recent years, discussed the microcosmic mechanism of DNA strand replacement reaction, and introduced the latest achievements in DNA molecular computing and its application in DNA constant temperature self-assembly.

The problem in this study is that most students lack basic motion in the rounders game. The effort taken is to implement a pairing learning strategy. The purpose of this study was to improve the basic mobility of rounders through pairing strategies in the fifth grade of SD Pacciro Elementary School, Ajangale District, Bone District 2017/2018 Academic Year. The object of the research is an increase in ball catching abilities in Rounders ball games. Research time for 6 months. Data collection techniques using observation, test and documentation methods. The data that has been obtained is then presented using descriptive statistics. From the results of classroom action research carried out that the application of the pairing learning strategy had a positive impact on improving the scholarship throwing ability of the fifth grade of SD Negeri 112 Pacciro Ajangale Subdistrict Bone District 2017/2018 Academic Year, this can be proven to increase student learning outcomes characterized by increased yields and completeness of student learning in each cycle. So it can be concluded that the application of paired learning strategies is proven to be able to improve the basic motion ability of ball catching at rounders game CLASS V SD Negeri 112 Pacciro Ajangale District Bone District 2017/2018 Academic Year.

We review our recent efforts in understanding the elastic properties of double-stranded (ds) and single-stranded (ss) DNA macromolecules. A simple geometric model of dsDNA was constructed and solved by path integral methods. The good agreement with experiments on dsDNA's entropic elasticity, cooperative extensibility, and supercoiling property suggested that the short-ranged base-pair stacking interaction is crucial for the stability and the high deformability of dsDNA. For ssDNA at high ionic conditions, base-pairing and base-pair stacking interactions cause the polymer to fold into compact hairpin configurations. The force-induced hairpin-coil transition was studied with the generating function method. In accordance with experiment, this transition was found to be highly cooperative when the average stacking potential is higher than some critical level. At low ionic conditions, the electrostatic repulsive interaction along the ssDNA becomes dominant, and ssDNA can be regarded as model polyelectrolytes. Our MC simulation results suggested an exponential relation between external force and the total extension. This prediction was confirmed experimentally.

Along with the development of the economy and increasingly advanced technology, companies are required to further increase production yields and product quality. The purpose of this study was to determine the effect of Competitive Advantage, Market Orientation and Entrepreneurship Orientation on Marketing Performance at the shuttlecock industry center in Sukoharjo Regency. This research is a quantitative research. The approach method in this study uses a quantitative survey approach, namely survey research that has the nature of pairing and is generally used to draw conclusions on the population so that it uses hypotheses and statistical tools in data analysis. The population and sample in this study were 37 players in the shuttlecock industry, data taken from the Central Bureau of Statistics of Gadingan village. The sampling technique used is the census method, which is a sampling technique when all members of the population are used as samples. 
 The results in this study are Competitive Advantage, Market Orientation, Entrepreneurship Orientation affect the Marketing Performance of the Shuttlecock Industrial Center in Sukoharjo Regency.
 
 Keywords: Competitive Advantage, Market Orientation, Entrepreneurship Orientation, Marketing Performance.
 
 ABSTRAK
 Seiring perkembangan perekonomian dan teknologi yang semakin maju, perusahaan dituntut agar lebih meningkatkan hasil produksi dan mutu produk yang dihasilkan. Tujuan penelitian ini adalah untuk mengetahui pengaruh Keunggulan Bersaing, Orientasi Pasar dan Orientasi Kewirausahaan terhadap Kinerja Pemasaran pada sentra industri shuttlecock di Kabupaten Sukoharjo. Penelitian ini merupakan penelitian kuantitatif. Metode pendekatan dalam penelitian ini menggunakan pendekatan kuantitatif survei, yaitu penelitian survei yang memiliki sifat pemairan dan umumnya digunakan untuk menarik kesimpulan terhadap populasi sehingga menggunakan hipotesis dan alat statistik dalam analisis data. Populasi dan sampel dalam penelitian ini adalah 37 pelaku industri shuttlecock, data diambil dari Badan Pusat Statistik desa Gadingan. Teknik pengambilan sampel dengan menggunakan metode sensus, yaitu teknik penentuan sampel bila semua anggota populsi digunakan sebagai sampel. 
 Hasil dalam penelitian ini adalah Keunggulan Bersaing, Orientasi Pasar, Orientasi Kewirausahaan berpengaruh terhadap Kinerja Pemasaran Sentra Industri Shuttlecock di Kabupaten Sukoharjo.
 
 Kata Kunci : Keunggulan Bersaing, Orientasi Pasar, Orientasi Kewirausahaan, Kinerja Pemasaran.

Abstract We report an approach to predict DNA specificity of the tetracycline repressor (TetR) family transcription regulators (TFRs). First, a genome sequence-based method was streamlined with quantitative P-values defined to filter out reliable predictions. Then, a framework was introduced to incorporate structural data and to train a statistical energy function to score the pairing between TFR and TFR binding site (TFBS) based on sequences. The predictions benchmarked against experiments, TFBSs for 29 out of 30 TFRs were correctly predicted by either the genome sequence-based or the statistical energy-based method. Using P-values or Z-scores as indicators, we estimate that 59.6% of TFRs are covered with relatively reliable predictions by at least one of the two methods, while only 28.7% are covered by the genome sequence-based method alone. Our approach predicts a large number of new TFBs which cannot be correctly retrieved from public databases such as FootprintDB. High-throughput experimental assays suggest that the statistical energy can model the TFBSs of a significant number of TFRs reliably. Thus the energy function may be applied to explore for new TFBSs in respective genomes. It is possible to extend our approach to other transcriptional factor families with sufficient structural information.

Penelitian ini berjudul pengaruh latihan beban menggunakan karet terhadap hasil tendangan dalam permainan sepak bola pada siswa putra kelas VIII SMP Negeri 1 Indralaya Selatan. Penelitian ini dilaksanakan pada tanggal 01 September sampai dengan 10 Oktober 2018. Penelitian ini termasuk penelitian eksperimen dengan menggunakan rancangan pretest-posttest control group design dengan variabel bebas latihan beban menggunakan karet, variabel terikat hasil tendangan dalam permainan sepak bola. Subjek dalam penelitian ini adalah siswa putra kelas VIII siswa yang dipilih dengan teknik Sampling. Penelitian ini bertujuan untuk mengetahui seberapa besar pengaruh latihan beban menggunakan karet terhadap hasil tendangan dalam permainan sepak bola pada siswa putra kelas VIII SMP Negeri 1 Indralaya Selatan, untuk menentukan kelompok eksperimen dan kontrol peneliti menggunakan teknik simple random sampling dengan sistem ordinal pairing. Metode pengumpulan data yang digunakan adalah tes, pada setiap kelompok ,tes diberikan di awal sebelum perlakuan dan di akhir setelah diberi perlakuan. Perlakuan di kelompok eksperimen berupa latihan beban menggunakan karet, Setelah diberikan perlakuan selama 6 minggu dengan frekuensi latihan 3 kali seminggu. Berdasarkan analisis data dengan cara statistik uji t dengan taraf signifikan α = 0, 05 di dapat t hitung = 31,92 dan t tabel = 1,67, apabila thitung > ttabel maka Ha diterima dan Ho ditolak. Dengan demikian Ha yang diajukan dapat diterima kebenarannya, bahwa latihan beban menggunakan karet berpengaruh terhadap hasil tendangan dalam permaina sepak bola pada siswa putra kelas VIII SMP Negeri 1 Indralaya Selatan.

Penelitian ini bertujuan untuk mengetahui: (1) pengaruh metode sport massage terhadap denyut nadi pemulihanp asca latihan, (2) pengaruh cold water immersion terhadap denyut nadi pemulihan pasca latihan. (3) perbedaan pengaruh antara metode sport massage dan cold water immersion terhadap denyut nadi pemulihan pasca latihan. Penelitian ini menggunakan metode eksperimen dengan pretest-posttest-control group design. Populasi dalam penelitian ini adalah tim bola voli di SMA Negeri 5 Purwokerto yang berjumlah 30 siswa. Teknik sampling yang digunakan adalah Total Sampling dan pembagian kelompok dengan model Ordinal Pairing, besar sampel yang digunakan adalah sebanyak 30 siswa yang dibagi menjadi 3 kelompok perlakuan . Teknik pengumpulan data dilakukan dengan tes dan pengukuan dalam olahraga menggunakan circuit training test dan pengukuran denyut nadi. Teknik analisis data dilakukan dengan analisis statistik menggunakan uji paired t-test dan uji independent t test, untuk memenuhi asumsi hasil penelitian dilakukan uji persyaratan analisis yaitu dengan uji normalitas dan uji homogenitas. Berdasarkan hasil analisis data, maka penelitian ini menghasilkan kesimpulan sebagai berikut: (1) ada pengaruh metode sport masage terhadap denyut nadi pemulihan pasca latihan (2) ada pengaruh metode cold water immersion terhadap denyut nadi pemulihan pasca latihan. (3) tidak ada perbedaan pengaruh yang signifikan antara metode sport massage dan cold water immersionterhadap denyut nadi pemulihan pasca latihan. EFFECT OF SPORT MASSAGE AND COLD WATER IMMERSION ON POST-EXERCISE PULSE IN VOLLEYBALL TEAMSAbstractThis study aims to determine: (1) The Effect of the Sports Massage Method on Post-Exercise Fatigue Recovery, (2) The Effect of Cold Water Immersion on Post-Exercise Fatigue Recovery. (3) Difference Between Influence Between Sports Massage Method and Cold Water Immersion on Post-Exercise Fatigue Recovery. This study uses an experimental method with a pretest-posttest-control group design. The population in this study was the volleyball team at SMA Negeri 5 Purwokerto, with a total of 30 students. The sampling technique used was total sampling and group division with Ordinal Pairing models, the sample size used was 30 students divided into 3 treatment groups. Data collection techniques were carried out with tests and bookkeeping in sports using a circuit training test and pulse measurement. The data analysis technique was carried out by statistical analysis using the paired t-test and independent t-test, to meet the assumptions of the results of the study the analysis of the test requirements was carried out with the normality and homogeneity tests. Based on the results of data analysis, this study produces the following conclusions: (1) There is an effect of the Sports Massage Method on Post-Exercise Fatigue Recovery. (2) There is an effect of the Cold Water Immersion Method on Post-Exercise Fatigue Recovery. (3) There is no significant difference in effect between the methods of Sports Massage and Cold Water Immersion on Post-Exercise Fatigue Recovery.

Abstract Motivation G-quadruplex is a DNA or RNA form in which four guanine-rich regions are held together by base pairing between guanine nucleotides in coordination with potassium ions. G-quadruplexes are increasingly seen as a biologically important component of genomes. Their detection in vivo is problematic; however, sequencing and spectrometric techniques exist for their in vitro detection. We previously devised the pqsfinder algorithm for PQS identification, implemented it in C++ and published as an R/Bioconductor package. We looked for ways to optimize pqsfinder for faster and user-friendly sequence analysis. Results We identified two weak points where pqsfinder could be optimized. We modified the internals of the recursive algorithm to avoid matching and scoring many sub-optimal PQS conformations that are later discarded. To accommodate the needs of a broader range of users, we created a website for submission of sequence analysis jobs that does not require knowledge of R to use pqsfinder. Availability and implementation https://pqsfinder.fi.muni.cz, https://bioconductor.org/packages/pqsfinder. Supplementary information Supplementary data are available at Bioinformatics online.

Fifty-five isolates of Eutypa lata were collected in 1994 from a single vineyard, each from a different vine that showed either shoot and foliar symptoms of Eutypa dieback or only abnormalities during the period of 1990 to 1994. These isolates showed a large variation in pathogenicity after inoculation on cuttings in the greenhouse. Variability also was observed for cultural traits and radial growth rate on potato dextrose agar (PDA) medium, but no relation was found between these characteristics and pathogenicity. The isolates were paired on PDA medium, and a barrage reaction was observed for all pairings, indicating the isolates were vegetatively incompatible. Thirty-two random amplified polymorphic DNA (RAPD) markers were used to study the genetic relatedness of the isolates and the genetic structure of the population. Fifty-five different RAPD patterns were observed, confirming the genetic uniqueness of each isolate. Gametic disequilibrium values were calculated among all pairs of the 32 putative RAPD loci, and only one value was significant. Unweighted pair-group method with arithmetic averages analysis of distance data, as well as a heterogeneity statistic (Fst), did not indicate any population substructure. The results strongly suggest that isolates originated from a random-mating population and that the disease was propagated in this vineyard by ascospores produced from diverse outside sources. Southern hybridization performed for five markers indicated that the two-allele assumption made to interpret RAPD data may be violated for markers that are similar in size. However, the exclusion of such markers did not change the conclusions of the study.

In the last few years it has been shown that the spontaneous self-assembly process of short DNA and RNA duplexes into liquid crystal ordering is a likely potential route that led to the formation of first nucleic acids able to support biological activities. In particular, it has been experimentally demonstrated that liquid crystal domains behave as suitable micro-reactors to trigger polymerization between the stacked and not initially chemically linked short nucleic acids. Even paired mononucleotides at high enough concentration exhibit liquid crystal ordering, unveiling the crucial role of Watson-Crick selectivity and stacking attractive interactions among base pairs. In such a possible prebiotic context, DNA sequences with both random nucleobases sequence and length are likely to be formed. Surprisingly, it has been shown that even random DNA sequence of fixed length can support liquid crystal ordering at high concentration. The aim of this PhD thesis is to extend the knowledge of DNA liquid crystals self-assembly in the following four directions. First, I explored the selectivity of interaction in nucleic acids solutions of random-sequence DNA oligomers of different length L. The combination of experimental results and a suitable developed theoretical model revealed a not negligible percentage of perfect duplexes. Second, I investigated the process that leads to the onset of the nematic liquid crystal phase in aqueous solutions of DNA duplexes. The combination of static light scattering experiments and computer simulations made possible the study of both aggregation and local ordering of DNA duplexes in the isotropic phase, where no positional order is developed, and in proximity of the isotropic-nematic phase boundary. This study gives an insight of the role on the development of local orientational order among DNA duplexes both far and in proximity of the isotropic-nematic phase boundary. Third, I studied the diffusion of short DNA duplexes with attractive and repulsive interactions in the isotropic phase as a function of temperature. I found that the temperature dependence of diffusion coefficients reflects via an Arrhenius law the interduplex attractive interactions, whereas diffusion of repulsive duplexes is partially well described in terms of repulsive hard spheres. Fourth, I investigated phase diagrams of mixtures of DNA single strands and duplexes with various polycations that show liquid-liquid phase separations. This phenomena leads to the onset of a concentrated but still liquid phase of polyelectrolytes, called coacervate, in a bulk phase where polyelectrolytes are diluted. The most surprising result I found, it is the insurgence of liquid crystals in coacervates with 12 nucleobases long random DNA oligomers and polylysine at different ionic strengths. I believe that this PhD thesis adds important pieces to the self-assembly of nucleic acids puzzle, and in particular it shows how randomness of nucleic acids is not an impasse to both hybridization of defectless duplexes and liquid crystal ordering.

DNA is a deformable molecule. The term “deformable” implies phenomena rooted in the collective behavior of many atoms, and a description based on concepts of continuum and statistical mechanics. Long DNA molecules are an excellent experimental system to study the equilibrium conformations and dynamics of long, flexible molecules. This chapter discusses the following: DNA melting, the zipper model, experimental melting curves, base pairing and base stacking as separate, Hamiltonian formulation of the zipper model, 2 × 2 model, nearest neighbor model, connection to nonlinear dynamics, linear and nonlinear elasticity of DNA, bending modulus and persistence length, measurements of DNA elasticity (short and long molecules), the Euler instability, and the DNA yield transition.