Bibliographies thématiques / DNA hybrid

Sommaire

  1. Articles de revues
  2. Thèses
  3. Livres
  4. Chapitres de livres
  5. Actes de conférences
  6. Rapports d'organisations

Littérature scientifique sur le sujet « DNA hybrid »

Auteur : Grafiati
Publié le 9 mars 2023
Mis à jour le 1 avril 2023

Créez une référence correcte selon les styles APA, MLA, Chicago, Harvard et plusieurs autres

Choisissez une source :

Consultez les listes thématiques d’articles de revues, de livres, de thèses, de rapports de conférences et d’autres sources académiques sur le sujet « DNA hybrid ».

À côté de chaque source dans la liste de références il y a un bouton « Ajouter à la bibliographie ». Cliquez sur ce bouton, et nous générerons automatiquement la référence bibliographique pour la source choisie selon votre style de citation préféré : APA, MLA, Harvard, Vancouver, Chicago, etc.

Vous pouvez aussi télécharger le texte intégral de la publication scolaire au format pdf et consulter son résumé en ligne lorsque ces informations sont inclues dans les métadonnées.

Articles de revues sur le sujet "DNA hybrid"

1

Kawashima, Etsuko, Yusuke Ohba, Yusuke Terui et Kazuo Kamaike. « Design, Synthesis, and Analysis of Minor Groove Binder Pyrrolepolyamide-2′-Deoxyguanosine Hybrids ». Journal of Nucleic Acids 2010 (2010) : 1–13. http://dx.doi.org/10.4061/2010/235240.

Texte intégral
Résumé :
Pyrrolepolyamide-2′-deoxyguanosine hybrids (Hybrid2and Hybrid3) incorporating the 3-aminopropionyl or 3-aminopropyl linker were designed and synthesized on the basis of previously reported results of a pyrrolepolyamide-adenosine hybrid (Hybrid1). Evaluation of the DNA binding sequence selectivity of pyrrolepolyamide-2′-deoxyguanosine hybrids was performed by CD spectral andTmanalyses. It was shown that Hybrid3possessed greater binding specificity than distamycin A, Hybrid1and Hybrid2.
Styles APA, Harvard, Vancouver, ISO, etc.
2

Tseng, Yu-Hsin, et Jer-Ming Hu. « A new hybrid from Taiwan, Elatostema ×hybrida (Urticaceae), is the first confirmed natural hybrid for Urticaceae ». Phytotaxa 161, no 1 (20 février 2014) : 43. http://dx.doi.org/10.11646/phytotaxa.161.1.2.

Texte intégral
Résumé :
Explosive pollen dispersal is common in Urticaceae and they are thought to be wind-pollinated. Despite a lack of obvious mechanism for preventing cross-species pollination, putative hybrid species in Urticaceae are rarely documented. Here we described the first natural hybrid in Urticaceae Elatostema ×hybrida from Taiwan. Morphological characters in E. ×hybrida are intermediate between putative parental species: E. lineolatum var. majus and E. platyphylloides. Six hybrid populations of E. ×hybrida were found in Taiwan that exhibited largely overlapping distribution patterns with its putative parents. Phylogenetic analysis of chloroplast DNA showed that the hybrid species is more closely related to E. lineolatum var. majus suggesting that the latter is the maternal parent and that hybridization is unidirectional. The chromosome number of E. ×hybrida remains the same as its putative parents (2n = 26). We speculate that the examined hybrids are natural first-generation results of independent hybridization events. Based on the morphology, spatial distribution, DNA sequence data, pollen viability and cytological observations, we hypothesize that E. ×hybrida is derived from natural hybridization events between E. lineolatum var. majus (♀) and E. platyphylloides (♂).
Styles APA, Harvard, Vancouver, ISO, etc.
3

KAPER, Thijs, Stan J. J. BROUNS, Ans C. M. GEERLING, Willem M. DE VOS et John VAN der OOST. « DNA family shuffling of hyperthermostable β-glycosidases ». Biochemical Journal 368, no 2 (1 décembre 2002) : 461–70. http://dx.doi.org/10.1042/bj20020726.

Texte intégral
Résumé :
The structural compatibility of two hyperthermostable family 1 glycoside hydrolases, Pyrococcus furiosus CelB and Sulfolobus solfataricus LacS, as well as their kinetic potential were studied by construction of a library of 2048 hybrid β-glycosidases using DNA family shuffling. The hybrids were tested for their thermostability, ability to hydrolyse lactose and sensitivity towards inhibition by glucose. Three screening rounds at 70°C led to the isolation of three high-performance hybrid enzymes (hybrid 11, 18 and 20) that had 1.5—3.5-fold and 3.5—8.6-fold increased lactose hydrolysis rates compared with parental CelB and LacS respectively. The three variants were the result of a single crossover event, which gave rise to hybrids with a LacS N-terminus and a main CelB sequence. Constructed three-dimensional models of the hybrid enzymes revealed that the catalytic (βα)8-barrel was composed of both LacS and CelB elements. In addition, an extra intersubunit hydrogen bond in hybrids 18 and 20 might explain their superior stability over hybrid 11. This study demonstrates that extremely thermostable enzymes with limited homology and different mechanisms of stabilization can be efficiently shuffled to form stable hybrids with improved catalytic features.
Styles APA, Harvard, Vancouver, ISO, etc.
4

Xu, B., et D. A. Clayton. « A persistent RNA-DNA hybrid is formed during transcription at a phylogenetically conserved mitochondrial DNA sequence. » Molecular and Cellular Biology 15, no 1 (janvier 1995) : 580–89. http://dx.doi.org/10.1128/mcb.15.1.580.

Texte intégral
Résumé :
Critical features of the mitochondrial leading-strand DNA replication origin are conserved from Saccharomyces cerevisiae to humans. These include a promoter and a downstream GC-rich sequence block (CSBII) that encodes rGs within the primer RNA. During in vitro transcription at yeast mitochondrial replication origins, there is stable and persistent RNA-DNA hybrid formation that begins at the 5' end of the rG region. The short rG-dC sequence is the necessary and sufficient nucleic acid element for establishing stable hybrids, and the presence of rGs within the RNA strand of the RNA-DNA hybrid is required. The efficiency of hybrid formation depends on the length of RNA synthesized 5' to CSBII and the type of RNA polymerase employed. Once made, the RNA strand of an RNA-DNA hybrid can serve as an effective primer for mitochondrial DNA polymerase. These results reveal a new mechanism for persistent RNA-DNA hybrid formation and suggest a step in priming mitochondrial DNA replication that requires both mitochondrial RNA polymerase and an rG-dC sequence-specific event to form an extensive RNA-DNA hybrid.
Styles APA, Harvard, Vancouver, ISO, etc.
5

Koroma, Alie Patrick, Raymond Jones et Pawel Michalak. « Snapshot of DNA methylation changes associated with hybridization in Xenopus ». Physiological Genomics 43, no 22 (novembre 2011) : 1276–80. http://dx.doi.org/10.1152/physiolgenomics.00110.2011.

Texte intégral
Résumé :
Hybridization often results in dramatic genome reconfigurations including epigenetic changes that control gene expression. Here we survey methylation patterns of interspecific Xenopus F1 hybrids relative to parental species X. laevis and X. muelleri, using methyl-sensitive amplification polymorphisms (MSAPs). Out of a total of 546 MSAP markers, 364 were effective in elucidating the difference in methylation patterns between the hybrids and the parental species. Principal coordinate analysis of methylated fragments revealed four distinct clusters with the two parental species separate from hybrid males and females. On average, hybrids were characterized by a higher proportion (70.6%) of methylated fragments compared with the parental species (64.5%), and this difference was consistent with previously observed disruptions of hybrid transcriptomes. The proportion of methylated fragments did not correlate with variation in genome size, as measured with flow cytometry. The levels of methylation in sterile hybrid males (73.8%) were higher than in fertile hybrid females (68.6%), but this difference was not statistically significant. A total of 76 methylated fragments (20.9%) were hybrid-unique, presumably originating from methylation alterations in hybrid genomes.
Styles APA, Harvard, Vancouver, ISO, etc.
6

Mangalam, Anand P., John Simonsen et Albert S. Benight. « Cellulose/DNA Hybrid Nanomaterials ». Biomacromolecules 10, no 3 (9 mars 2009) : 497–504. http://dx.doi.org/10.1021/bm800925x.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
7

Cheng, Enjun, Yulin Li, Zhongqiang Yang, Zhaoxiang Deng et Dongsheng Liu. « DNA-SWNT hybrid hydrogel ». Chemical Communications 47, no 19 (2011) : 5545. http://dx.doi.org/10.1039/c1cc11028d.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
8

Rioz-Martínez, Ana, et Gerard Roelfes. « DNA-based hybrid catalysis ». Current Opinion in Chemical Biology 25 (avril 2015) : 80–87. http://dx.doi.org/10.1016/j.cbpa.2014.12.033.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
9

KONSTANTINOVIC, MIROSLAV, VESNA MAKSIMOVIC, GORDANA NIKCEVIC et VLADIMIR GLISIN. « Hybrid PLtl Promoter with Dual Regulation Control ». DNA and Cell Biology 10, no 5 (juin 1991) : 389–95. http://dx.doi.org/10.1089/dna.1991.10.389.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
10

SUYAMA, AKIRA. « DNA Computing. 4. Hardware of Hybrid DNA Computer. » Journal of the Institute of Electrical Engineers of Japan 122, no 3 (2002) : 160–63. http://dx.doi.org/10.1541/ieejjournal.122.160.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
Plus de sources

Thèses sur le sujet "DNA hybrid"

1

Farrow, Paul J. « Development of hybrid mRNA/DNA vectors for gene therapy ». Thesis, University of Oxford, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.426407.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
2

Novoa, Carolina. « RecQ-like helicase SGS1 counteracts DNA : RNA hybrid induced genome instability ». Thesis, University of British Columbia, 2017. http://hdl.handle.net/2429/60964.

Texte intégral
Résumé :
Dividing cells are constantly under threat from both endogenous and exogenous DNA damaging stresses that can lead to mutations and structural variations in DNA. One contributor to genome instability is three-stranded DNA:RNA hybrid structures called R-loops. Though R-loops are known to induce DNA damage and DNA replication stress, it is unclear whether they are recognized and processed by an established DNA repair pathway prior to inducing DNA breaks. Canonically, DNA repair proteins work downstream of R-loop-induced DNA damage to stimulate repair and suppress genome instability. Recently, the possibility that some DNA repair pathways actively destabilize R-loops, thus preventing unscheduled DNA damage has emerged. Here we identify the helicase SGS1 as a suppressor of R-loop stability. Our data reveals that SGS1 depleted cells accumulate R-loops. In addition, we define a role for transcription in genome instability of cells lacking SGS1, which is consistent with an R-loop based mechanism. Hyper-recombination in SGS1 mutants is dependent on transcript length, transcription rate, and active DNA replication. Also, rDNA instability in sgs1Δ can be suppressed by ectopic expression of RNaseH1, a protein that degrades DNA:RNA hybrids. Interestingly, R-loops are known to form at rDNA loci. We favour a model in which SGS1 contributes to the stabilization of stalled replication forks associated with transcription complexes, and unresolved DNA:RNA hybrids. Finally, we showed that knockdown of the human Sgs1 orthologue BLM in HCT116 cells also led to the accumulation of more R-loops than control HCT116 cells. In summary, our data supports the idea that some DNA repair proteins involved in replication fork stabilization might also prevent and process R-loops.
Science, Faculty of
Graduate
Styles APA, Harvard, Vancouver, ISO, etc.
3

Käslin, Edgar. « In vitro hybrid DNA formation by proteins from vegetative Schizosaccharomyces pombe cells / ». [S.l : s.n.], 1993. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
4

Patel, Chandan. « Hybrid molecular simulations of oxidative complex lesions ». Thesis, Lyon, École normale supérieure, 2013. http://www.theses.fr/2013ENSL0835.

Texte intégral
Résumé :
L'ADN est en permanence exposé à un grand nombre d'événements dommageables déclenchées par des agents endogènes et exogènes. De nombreux travaux expérimentaux ont fourni des informations cruciales sur les propriétés structurelles et la réparation de certains des lésions de l'ADN. Cependant, il manque une vision mécanistique ou énergétique sur leur formation. La biochimie computationnelle a émergé comme un outil puissant pour comprendre les réactions biochimiques et les propriétés électroniques de systèmes complexes.Dans cette thèse, nous étudions la formation de lésions complexes intra-brin et inter-brin. Ces lésions tandem constituent une puissant menace à l'intégrité du génome, en raison de leur haute fréquence mutagenique. Tout d'abord, nous discutons l'attaque d'une liaison covalente entre un radical pyrimidinique. En comparant avec les bases isolees, nos simulations hybrides Car-Parrinello demontrent que la reactivité de la thymine et de la cytosine radicalaires sont inversees dans l'environnement B-helical. De plus, nos resultats montrent egalement une deformation plus importante pour la lesion G[8-5]C.Nous rationalisons également la plus grande réactivité des cytosines par rapport aux purines vers la formation multi-etapes de lésions complexes inter-brins par condensation avec un site C4' abasique. Ces résultats bases sur des simulations avec solvatation explicite et combines a la théorie de la fonctionnelle de la densité sont en accord avec les données expérimentales
DNA is continuously exposed to a vast number of damaging events triggered by endogenous and exogenous agents. Numerous experimental studies have provided key information regarding structural properties of some of the DNA lesions and their repair. However, they lack in mechanistic or energetic information pertaining to their formation. Computational Biochemistry has emerged as a powerful tool to understand biochemical reactions and electronic properties of large systems.In this thesis we study the formation of inter- and intra-strand cross-links. These tandem lesions pose a potent threat to genome integrity, because of their high mutagenic frequency. First, we discuss the formation of complex defects which arise from the attack of a pyrimidine radical onto guanine. In comparison with the reactivity of isolated nucleobases, our hybrid Car-Parrinello Molecular Dynamics simulations reveal that the reactivity of hydrogen-abstracted thymine and cytosine is reversed within a B-helix environment. Further, our data also suggest a more severe distortion of the B-helix for G[8-5]C.Second, we rationalize the higher reactivity of cytosine vs. purines toward the multistep formation of inter-strand crosslinks with a C4' oxidized a basic site, which is in qualitative agreement with experiments on isolated nucleobases, using explicit solvent simulations combined to density functional theory
Styles APA, Harvard, Vancouver, ISO, etc.
5

Diallo, Amy. « The DNA translocation apparatus involved in Streptococcus Pneumoniae transformation ». Thesis, Paris 6, 2016. http://www.theses.fr/2016PA066334/document.

Texte intégral
Résumé :
La transformation naturelle bactérienne permet aux micro-organismes d'échanger des informations génétiques pour promouvoir leurs réponses adaptatives pour faire face aux changements environnementaux. De l'ADN extracellulaire est incorporé et recombiné au génome de l'hôte. Ce processus augmente la plasticité des bactéries. Chez S. pneumoniae, un pathogène majeur chez l'Homme engendrant des infections pouvant être mortelles, la transformation bactérienne accentue la transmission de gènes de résistance aux antibiotiques. Chez les bactéries à Gram positif, l'opéron comF encode l'expression de deux protéines. L'une est démontrée comme étant essentielle à la transformation, est décrite pour être membranaire. La seconde n'a pas été étudiée. Cependant ces protéines n'ont pas été étudiées d'un point de vue structural ou fonctionnel. Des mutagenèse et le double hybride bactérien ont permis de mettre en évidence que ses protéines sont indispensables pour l'expression de la compétence et interagissent avec de nombreuses protéines du transformasome. De plus, l'expression des deux protéines de manière hétérologue prouve qu'elles sont solubles et forment des oligomères. L'analyse structurale de ComFA, atteste de la conformation atypique de cette helicase trimerique et hexamerique. En outre, l'activité ATPasique simple brin DNA-dépendant de cette protéine est démontrée. Finalement un complexe protéique a été révélé entre ComFA et ComFC dont l'étude microscopique à hautes résolutions prouve l'apparition d'un anneau via l'assemblage de deux hexamères. Ces résultats suggèrent que ComFA est le moteur tirant l'ADN dans la cellule. Quant à ComFC, elle semble aider à la stabilisation de ComFA
Bacterial natural transformation allows microorganisms to exchange genetic information to promote their adaptive responses to cope with environmental changes. The extracellular DNA is incorporated and recombined with the genome of the host. This phenomenon increases the plasticity of Gram positive and negative bacteria. S. pneumoniae is a major pathogen for humans, which is causing infections that can be deadly. In this specie, bacterial transformation increases the transmission of antibiotic resistance.In Gram-positive bacteria, comF operon encodes the expression of two proteins. One of them, shown to be essential for natural transformation, is expected to be a membrane protein. The second is not described. However, up to now neither protein has been studied from a structural or functional point of view. Mutagenesis technique and double hybrid bacterial assay allowed to show that both proteins are essential for the expression of the competence and interact with many proteins of the transformasome. In addition, heterologous expresion of both proteins have shown their solubility and the formation of oligomers. Structural analysis of ComFA demonstrates the unique conformation of this hexameric and trimeric helicase. Furthermore, the ATPase single stranded DNA-dependent activity of this protein could be detected. Finally, a protein complex is formed between ComFA and ComF, and high-resolution microscopic study proves the occurrence of a ring via a two-hexamers. These results suggest that ComFA is the engine pulling the DNA in the cell. As for ComFC, this protein seems to help stabilizing of ComFA
Styles APA, Harvard, Vancouver, ISO, etc.
6

Kumar, Deepak. « Analysis and confirmation of the results of a yeast two-hybrid screen carried out to identify proteins that interact with drosophila XRCC2 ». Scholarly Commons, 2005. https://scholarlycommons.pacific.edu/uop_etds/622.

Texte intégral
Résumé :
Repairing DNA damage is brought about by highly specific proteins that partake in a variety of DNA repair. Two of the most common types of damage are double-strand breaks (DSBs) and interstrand crosslinks. A single DSB or crosslink can potentially kill a cell if it is not repaired~ In human and other vertebrate cells, DSBs are repaired by two different mechanisms. The nonhomologous end-joining pathway can bring together the broken ends and join them, usually with the loss of some nucleotide sequence. A second pathway, homologous recombinational repair (HRR), is equally important. This repair process utilizes the information provided by another DNA molecule to restore damaged DNA. This molecule is usually a sister chromatid arising from DNA replication. This process is essentially error-free, unlike the end-joining process. Some HRR activity is required for proliferating cells to remain viable. The central protein player is RAD51, which with the help of other proteins such as XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D, performs the critical initiating steps of homologous pairing and strand transfer. The proteins encoded by the familial breast cancer genes, brcal and brca2, also play an important role in HRR. My project is concerned with studying proteins that interact with Drosophila melanogaster (XRCC2). Proteins interacting with DmXRCC2 were identified by using a yeast two hybrid system. "Bait fusion protein" (DmXRCC2 linked to GAIA BD) was constructed by Dr. Wrischnik. Tanya Dimetrijevich, a graduate student, used this bait to fish for interacting or "target" proteins. About 50 such proteins were found. I began validating these target proteins with the intention of exploring novel interactions and functions of DmXRCC2. The process of validating proteins interacting with DmXRCC2 yielded two very interesting candidate proteins-CaBPl and FAF. · CaBPl, also called protein disulfide isomerase P5, is an endoplasmic-reticulum calciumbinding protein. FAF belongs to a large family of deubiquitinating enzymes that cleave ubiquitin-protein bonds and play diverse roles in the ubiquitin pathway. One of the implications of such discoveries could be to compare and contrast DmXRCC2 and human XRCC2 in terms of their interactions and functions.
Styles APA, Harvard, Vancouver, ISO, etc.
7

Kirkpatrick, Robert Daniel. « Interactions of the DNA repair protein Rad23 in the yeast two-hybrid system ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ45071.pdf.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
8

Joyce, Donna Marie. « The Development of DNA-Based Bio-Polymer Hybrid Thin Films for Capacitor Applications ». University of Dayton / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1389285491.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
9

Morley, Stewart Anthony. « Interactions Between the Organellar Pol1A, Pol1B, and Twinkle DNA Replication Proteins and Their Role in Plant Organelle DNA Replication ». BYU ScholarsArchive, 2019. https://scholarsarchive.byu.edu/etd/8128.

Texte intégral
Résumé :
Plants maintain organelle genomes that are descended from ancient microbes. Ages ago, these ancient microbes were engulfed by larger cells, beginning a process of co-evolution we now call the endo-symbiotic theory. Over time, DNA from the engulfed microbe was transferred to the genome of the larger engulfing cell, eventually losing the ability to be free-living, and establishing a permanent residency in the larger cell. Similarly, the larger cell came to rely so much on the microbe it had engulfed, that it too lost its ability to survive without it. Thus, mitochondria and plastids were born. Nearly all multicellular eukaryotes possess mitochondria; however, different evolutionary pressures have created drastically different genomes in plants versus animals. For one, animals have very compact, efficient mitochondrial genomes, with about 97% of the DNA coding for genes. These genomes are very consistent in size across different animal species. Plants, on the other hand, have mitochondrial genomes 10 to more than 100 times as large as animal mitochondrial genomes. Plants also use a variety of mechanisms to replicate and maintain their DNA. Central to these mechanisms are nuclear-encoded, organelle targeted replication proteins. To date, there are two DNA polymerases that have been identified in plant mitochondria and chloroplasts, Pol1A and Pol1B. There is also a DNA helicase-primase that localizes to mitochondria and chloroplasts called Twinkle, which has similarities to the gp4 protein from T7 phage. In this dissertation, we discuss the roles of the polymerases and the effects of mutating the Pol1A and Pol1B genes respectively. We show that organelle genome copy number decreases slightly and over time but with little effect on plant development. We also detail the interactions between Twinkle and Pol1A or Pol1B. Plants possess the same organellar proteins found in animal mitochondria, which are homologs to T7 phage DNA replication proteins. We show that similar to animals and some phage, plants utilize the same proteins in similar interactions to form the basis of a DNA replisome. However, we also show that plants mutated for Twinkle protein show no discernable growth defects, suggesting there are alternative replication mechanisms available to plant mitochondria that are not accessible in animals.
Styles APA, Harvard, Vancouver, ISO, etc.
10

Islam, Mohammad Kaisarul. « Novel ligands targeting the DNA/RNA hybrid and telomeric quadruplex as potential anticancer agents ». Thesis, King's College London (University of London), 2016. https://kclpure.kcl.ac.uk/portal/en/theses/novel-ligands-targeting-the-dnarna-hybrid-and-telomeric-quadruplex-as-potential-anticancer-agents(ce8f3d0e-317d-4c2e-b64a-e13e283f7b95).html.

Texte intégral
Résumé :
Telomeres are repetitive sequences of DNA at the ends of chromosomes that become progressively shorter during cell division, acting as a form of “biological clock” causing cell death once they have reached a certain length. Almost 90% of cancer cells overexpress the enzyme telomerase which can lengthen telomeres and confer immortality to the tumour cells. Thus, telomerase has become an important target for drug discovery in the oncology area, and there is also interest from researchers investigating the aging process. During the catalytic cycle of telomerase, a unique DNA/RNA hybrid duplex (DRH) forms that is typically between 6-11 base pairs long and is key to extending the telomere. There is interest in discovering small drug-like molecules that can recognize and bind to this hybrid duplex to inhibit selectively telomerase, either by stabilizing the structure and thereby preventing telomerase dissociation (a key step in the catalytic cycle) or by sufficiently distorting the hybrid duplex to cause the misalignment of key catalytic groups. This project began by using oligonucleotides representing DNA/RNA hybrid duplex (DRH), telomeric G-quadruplex and control duplex DNA sequences to screen against the National Cancer Institute compound libraries (i.e., Diversity Set II, Mechanistic Set and Natural Product Set) using a high throughput Fluorescent Resonance Energy Transfer (FRET)-based DNA thermal denaturation assay to determine binding affinity and specificity. Thirteen novel chemical scaffold families were identified in the assay, compounds which showed a >5 °C selective stabilization of the DNA/RNA hybrid duplex at a 1 μM ligand concentration. Chemical modifications were then made to these scaffolds to generate focused libraries of analogues to improve selectivity, potency and drug-likeness, and to provide Structure-Activity Relationship (SAR) information. A total of 49 novel molecules were synthesized and then screened against an expanded range of four different nucleic acid constructs including telomeric and DNA/RNA hybrid duplex sequences. A number of compounds showed selective DNA/RNA hybrid stabilization potential with some compounds also showing notable telomeric G-quadruplex stabilization without significant affinity for promoter G-quadruplexes (i.e., c-Kit1, c-Kit-2 and c-Myc) and control duplex DNA sequences. The compounds from library-1 provided DNA/RNA hybrid duplex stabilization in the 0.5-7.2 C range and telomeric G-quadruplex stabilization in the 0.2-6.5 C range at a 1 μM ligand concentration. Molecular modelling and molecular dynamics studies confirmed that the methylene spacer between the benzimidazole and phenylene moieties of molecules within library-1 is perfectly shaped to fit within the DRH sequence. In addition, it was confirmed that minor-groove binding and simultaneous intercalation between the nucleobases of a DNA/RNA hybrid duplex requires a linker of specific length (i.e., an eight methylene spacer as in compound 3.3). Selected compounds were then studied further using a variety of biological techniques to confirm selective telomerase inhibition and cell-based assays to utilize their potential as antitumour agents.
Styles APA, Harvard, Vancouver, ISO, etc.
Plus de sources

Livres sur le sujet "DNA hybrid"

1

Kingsbury, Noël. Hybrid. Chicago : University of Chicago Press, 2009.

Trouver le texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
2

Za jiao yu mi pin zhong DNA zhi wen tu pu : DNA finger print of maize hybrids. Beijing Shi : Zhongguo nong ye ke xue ji shu chu ban she, 2004.

Trouver le texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
3

Hybrid : The history and science of plant breeding. Chicago : The University of Chicago Press, 2009.

Trouver le texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
4

Banasik, Mirosław. Wojna hybrydowa i jej konsekwencje dla bezpieczeństwa euroatlantyckiego. Warszawa : Wydawnictwo "Difin", 2018.

Trouver le texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
5

Grochocka, Julia Anna. Wojna hybrydowa na Ukrainie : Wnioski i rekomendacje dla Europy i świata. Piotrków Trybunalski : Wydawnictwo Uniwersytetu Jana Kochanowskiego Filia w Piotrkowie Trybunalskim, 2017.

Trouver le texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
6

Arrabito, Giuseppe, et Liqian Wang. DNA Nanotechnology for Bioanalysis : From Hybrid DNA Nanostructures to Functional Devices. World Scientific Publishing Co Pte Ltd, 2017.

Trouver le texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
7

Analisis konsep desain hybrid, studi kasus, Masjid Fakultas Teknik UGM dan Masjid Agung Jawa Tengah : Laporan penelitian dosen muda. [Yogyakarta] : Fakultas Teknik, Universitas Negeri Yogyakarta, 2007.

Trouver le texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
8

Adolph, Kenneth W. Methods in Molecular Genetics : Human Molecular Genetics (Methods in Molecular Genetics). Academic Pr, 1996.

Trouver le texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
9

Adolph, Kenneth W. Methods in Molecular Genetics : Human Molecular Genetics (Methods in Molecular Genetics). Academic Pr, 1996.

Trouver le texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
10

Methods in Molecular Genetics : Gene and Chromosome Analysis, Part A (Methods in Molecular Genetics). Academic Press, 1993.

Trouver le texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.

Chapitres de livres sur le sujet "DNA hybrid"

1

Margenstern, Maurice, Victor Mitrana et Mario J. Pérez-Jiménez. « Accepting Hybrid Networks of Evolutionary Processors ». Dans DNA Computing, 235–46. Berlin, Heidelberg : Springer Berlin Heidelberg, 2005. http://dx.doi.org/10.1007/11493785_21.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
2

Blum, Christian, et Mateu Yábar Vallès. « Multi-level Ant Colony Optimization for DNA Sequencing by Hybridization ». Dans Hybrid Metaheuristics, 94–109. Berlin, Heidelberg : Springer Berlin Heidelberg, 2006. http://dx.doi.org/10.1007/11890584_8.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
3

Bock, Christoph, Luca Bortolussi, Thilo Krüger, Linar Mikeev et Verena Wolf. « Model-Based Whole-Genome Analysis of DNA Methylation Fidelity ». Dans Hybrid Systems Biology, 141–55. Cham : Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-26916-0_8.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
4

Kyriakopoulos, Charalampos, Pascal Giehr, Alexander Lück, Jörn Walter et Verena Wolf. « A Hybrid HMM Approach for the Dynamics of DNA Methylation ». Dans Hybrid Systems Biology, 117–31. Cham : Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-28042-0_8.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
5

Cao, Yuanyuan, et Shunai Che. « DNA Condensed Phase and DNA-Inorganic Hybrid Mesostructured Materials ». Dans ACS Symposium Series, 49–79. Washington, DC : American Chemical Society, 2017. http://dx.doi.org/10.1021/bk-2017-1252.ch004.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
6

Najaf Torkaman, Mohammad Reza, Pourya Nikfard, Nazanin Sadat Kazazi, Mohammad Reza Abbasy et S. Farzaneh Tabatabaiee. « Improving Hybrid Cryptosystems with DNA Steganography ». Dans Communications in Computer and Information Science, 42–52. Berlin, Heidelberg : Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-22603-8_4.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
7

Wheelhouse, Richard T., et Jonathan B. Chaires. « Drug Binding to DNA⋅RNA Hybrid Structures ». Dans Methods in Molecular Biology, 55–70. Totowa, NJ : Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-418-0_4.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
8

Kumar Saha, Sumit, et Md Rafiqul Islam. « DNA Motif Discovery Using a Hybrid Algorithm ». Dans Proceedings of International Joint Conference on Computational Intelligence, 275–85. Singapore : Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-3607-6_22.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
9

Liu, Sharong, Jianzhong Zhang, Hongji Ren, Jianbiao Zheng et Hanghui Liu. « A Microfabricated Hybrid Device for DNA Sequencing ». Dans Micro Total Analysis Systems 2001, 99–100. Dordrecht : Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-010-1015-3_37.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
10

Tao, Jili, Ridong Zhang et Yong Zhu. « DNA Double-Helix and SQP Hybrid Genetic Algorithm ». Dans DNA Computing Based Genetic Algorithm, 57–79. Singapore : Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-5403-2_3.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.

Actes de conférences sur le sujet "DNA hybrid"

1

Norwood, R. A., J. Thomas, N. Peyghambarian, J. Wang, L. Li, F. Ouchen et J. E. Grote. « Hybrid DNA materials for energy storage ». Dans SPIE NanoScience + Engineering, sous la direction de Norihisa Kobayashi, Fahima Ouchen et Ileana Rau. SPIE, 2010. http://dx.doi.org/10.1117/12.862412.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
2

Ogata, Naoya, Yoshiharu Kagami, Masahiro Wada et Junichi Yoshida. « DNA-hybrid materials for photonic applications ». Dans NanoScience + Engineering, sous la direction de Emily M. Heckman, Thokchom B. Singh et Junichi Yoshida. SPIE, 2007. http://dx.doi.org/10.1117/12.742131.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
3

Patel, Rashmit, et Rutu Parekh. « DNA Based Hybrid Circuit Design Approaches ». Dans 2019 IEEE 5th International Conference for Convergence in Technology (I2CT). IEEE, 2019. http://dx.doi.org/10.1109/i2ct45611.2019.9034066.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
4

Lin, Yueh-Cheng, Chi-Hsien Cheng et Yu-Chueh Hung. « Stimulus pulse-dependent responses in natural DNA biopolymer devices ». Dans Organic and Hybrid Sensors and Bioelectronics XIV, sous la direction de Ruth Shinar, Ioannis Kymissis et Emil J. List-Kratochvil. SPIE, 2021. http://dx.doi.org/10.1117/12.2593450.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
5

Kawabe, Yutaka. « Incorporation of photo-controllable molecules in tunable DNA dye laser system ». Dans Organic and Hybrid Sensors and Bioelectronics XI, sous la direction de Ruth Shinar, Ioannis Kymissis, Luisa Torsi et Emil J. List-Kratochvil. SPIE, 2018. http://dx.doi.org/10.1117/12.2320947.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
6

Han, Aili. « RLM : A New Method of Encoding Weights in DNA Strands ». Dans 2006 Sixth International Conference on Hybrid Intelligent Systems. IEEE, 2006. http://dx.doi.org/10.1109/his.2006.264900.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
7

Pike, Andrew R. « Integrating DNA With Semiconductor Materials : Bio-inorganic Hybrid Devices ». Dans DNA-BASED MOLECULAR CONSTRUCTION : International Workshop on DNA-Based Molecular Construction. AIP, 2002. http://dx.doi.org/10.1063/1.1520073.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
8

Han, Aili. « DNA Computing Model for the 0/1 Knapsack Problem ». Dans 2006 Sixth International Conference on Hybrid Intelligent Systems (HIS'06). IEEE, 2006. http://dx.doi.org/10.1109/his.2006.264901.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
9

Qinru Qiu, D. Burns, Qing Wu et P. Mukre. « Hybrid Architecture for Accelerating DNA Codeword Library Searching ». Dans 2007 4th Symposium on Computational Intelligence in Bioinformatics and Computational Biology. IEEE, 2007. http://dx.doi.org/10.1109/cibcb.2007.4221240.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
10

Perera, Kokila K., et C. Thusangi Wannige. « A hybrid algorithm for multiple DNA sequence alignment ». Dans 2016 Sixteenth International Conference on Advances in ICT for Emerging Regions (ICTer). IEEE, 2016. http://dx.doi.org/10.1109/icter.2016.7829939.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.

Rapports d'organisations sur le sujet "DNA hybrid"

1

Schikora, B., S. Hietala, L. Shi, L. Lee, E. Skowronski et A. Ardans. Hybrid Pathogen DNA Detector:Users ? Manual v1.5. Office of Scientific and Technical Information (OSTI), janvier 2004. http://dx.doi.org/10.2172/15014078.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
2

West, Abby L., Mark H. Griep, Dan P. Cole et Shashi P. Karna. Gold Nanocluster-DNase 1 Hybrid Materials for DNA Contamination Sensing. Fort Belvoir, VA : Defense Technical Information Center, janvier 2014. http://dx.doi.org/10.21236/ada610452.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
3

Humphries, David, Martin Pollard, Chris Elkin, Karl Petermann, Charles Reiter et Mario Cepeda. New high performance hybrid magnet plates for DNA separation andbio-technology applications. Office of Scientific and Technical Information (OSTI), août 2004. http://dx.doi.org/10.2172/861015.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
4

Dugan, L. Elucidation of the Mechanism of Gene Silencing using Small Interferin RNA : DNA Hybrid Molecules. Office of Scientific and Technical Information (OSTI), février 2006. http://dx.doi.org/10.2172/900164.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
5

Lin, Kuan-Jiuh, Watson Kuo et Ching-Hsiu Tsai. Hybrid Semiconductor Nanostructures as Unique Capabilities in the Direct Detection of Proteins, Viruses, and DNA. Fort Belvoir, VA : Defense Technical Information Center, février 2008. http://dx.doi.org/10.21236/ada476323.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
6

Sink, Ken, Shamay Izhar et Abraham Nachmias. Asymmetric Somatic Hybridization : Developing a Gene Transfer System for Solanaceous Vegetable Crops. United States Department of Agriculture, février 1996. http://dx.doi.org/10.32747/1996.7613010.bard.

Texte intégral
Résumé :
Highly asymmetric somatic hybrid plants were obtained by PEG/DMSO fusion of gamma irradiated (100, 250, 7500 and 1000 Gy) protoplasts of a (KmR-) interspecific hybrid Lycopersicon esculentum x L. pennellii (EP) with protoplasts of eggplant (E). Somatic hybrid calli were selected based on kanamycin resistance and verified by PCR of the NptII gene, RAPD's and Southern's using potato rDNA pTHG2 probes. Flow cytometry indicated all hybrid calli that did not regenerate shoots were 5-9n. Three asymmetric plants regenerated only from callus close to 4n and such calli oly occurred when EP received 100 Gy. The asymmetric plants had eggplant morphology and regenerated from one hybrid callus with 6.29 average size tomato chromosomes. Limited amounts of EP DNA were found in the three somatic hybrid plants H18-1 to -3 by dot-blot hybridization with probe pTHG2, to be equivalent to 6.23, 5.41, and 5.95 % EP, respectively. RFLP analysis of Lycopersicon esculentum and L. pennellii specific chromosomes revealed that only fragments of 8 to 10 out of the 24 EP chromosomes are present in the asymmetric plants. Transgenic plants 2-3, 2-4 and 10-3 were found resistant to verticillium; suggesting successful transfer of the Ve complex from S. torvum to eggplant.
Styles APA, Harvard, Vancouver, ISO, etc.
7

Reisch, Bruce, Pinhas Spiegel-Roy, Norman Weeden, Gozal Ben-Hayyim et Jacques Beckmann. Genetic Analysis in vitis Using Molecular Markers. United States Department of Agriculture, avril 1995. http://dx.doi.org/10.32747/1995.7613014.bard.

Texte intégral
Résumé :
Genetic analysis and mapping in grapes has been difficult because of the long generation period and paucity of genetic markers. In the present work, chromosome linkage maps were developed with RAPD, RFLP and isozyme loci in interspecific hybrid cultivars, and RAPD markers were produced in a V. vinifera population. In three cultivars, there were 19 linkage groups as expected for a species with 38 somatic chromosomes. These maps were used to locate chromosome regions with linkages to important genes, including those influencing powdery mildew and botrytis bunch rot resistance; flower sex; and berry shape. In V. vinifera, the occurrence of specific markers was correlated with seedlessness, muscat flavor and fruit color. Polymorphic RAPD bands included single copy as well as repetitive DNA. Mapping procedures were improved by optimizing PCR parameters with grape DNA; by the development of an efficient DNA extraction protocol; and with the use of long (17- to 24-mer) primers which amplify more polymorphic loci per primer. DNA fingerprint analysis with RAPD markers indicated that vinifera cultivars could be separated readily with RAPD profiles. Pinot gris, thought to be a sort of Pinot noir, differed by 12 bands from Pinot noir. This suggests that while Pinot gris may be related to Pinot noir, it is not likely to be a clone. The techniques developed in this project are now being further refined to use marker-assisted selection in breeding programs for the early selection of elite seedlings. Furthermore, the stage has been set for future attempts to clone genes from grapes based upon map locations.
Styles APA, Harvard, Vancouver, ISO, etc.
8

Tel-Zur, Neomi, et Jeffrey J. Doyle. Role of Polyploidy in Vine Cacti Speciation and Crop Domestication. United States Department of Agriculture, janvier 2012. http://dx.doi.org/10.32747/2012.7697110.bard.

Texte intégral
Résumé :
1. Abstract: Over the past 25 years, vine cacti of the genera Hylocereus and Selenicereus have been introduced into Israel and southern California as new exotic fruit crops. The importance of these crops lies in their high water use efficiency and horticultural potential as exotic fruit crops. Our collaboration focused on the cytological, molecular and evolutionary aspects of vine cacti polyploidization to confront the agricultural challenge of genetic improvement, ultimately to improve success of vine cacti as commercial fruit crop plants. More specifically, we worked on the: 1- Identification of the putative ancestor(s) of the tetraploid H. megalanthus; 2- Determination of the number of origins of H. megalanthus (single vs. multiple origins of polyploidy); 3- Cytogenetic analysis of BC1 and F1 hybrids; 4- Determination of important agricultural traits and the selection of superior hybrids for cultivation. The plant material used in this study comprised interspecific Hylocereus F1 and first backcross (BC1) hybrids, nine Hylocereus species (58 genotypes), nine Selenicereus species (14 genotypes), and four Epiphyllum genotypes. Two BC1 hexaploids (BC-023 and BC-031) were obtained, a high ploidy level that can be explained only by a fertilization event between one unreduced female gamete from the triploid hybrid and a balanced gamete from the pollen donor, the diploid H. monacanthus. These findings are scientific evidence that support the possibility that “hybridization followed by chromosome doubling” could also occur in nature. Cytomixis, the migration of chromatin between adjacent cells through connecting cytoplasmatic channels, was observed in vine cacti hybrids and may thus imply selective DNA elimination in response to the allopolyploidization process. Evidence from plastid and nrDNA internal transcribed spacers (ITS) sequences support the placement of H. megalanthus within a monophyletic Hylocereus group. Furthermore, both plastid and ITS datasets are most consistent with a conclusion that this tetraploid species is an autopolyploid, despite observations that the species appears to be morphologically intermediate between Hylocereus and Selenicereus. Although the possibility of very narrow allopolyploidly (i.e., derivation from parents that are barely diverged from each other such as closely related species in the same genus) cannot be ruled out entirely based on our data (in part due to the unavailability of Hylocereus species considered to be morphologically the closest relatives of H. megalanthus), the possibility of H. megalanthus representing an intergeneric cross (i.e., Hylocereus × Selenicereus) seems extremely unlikely. Interestingly, the process of homogenization of ITS sequences (concerted evolution) is either incomplete or lacking in both Hylocereus and Selenicereus, and the inclusion of several artificial hybrids in the molecular study revealed the potential for biparental plastid inheritance in Hylocereus. The most important agricultural implication of this research project was the information collected for F1 and BC1 hybrids. Specifically, this project concluded with the selection of four superior hybrids in terms of fruit quality and potential yields under extreme high temperatures. These selected hybrids are self-compatible, avoiding the need for hand cross pollination to set fruits, thus reducing manpower costs. We recently offered these hybrids to growers in Israel for prioritized rapid evaluation and characterization.
Styles APA, Harvard, Vancouver, ISO, etc.
9

Weller, Joel, Harris Lewin, Micha Ron, George Wiggans et Paul VanRaden. A Systematic Genome Search for Genes Affecting Economic Traits Dairy Cattle with the Aid of Genetic Markers. United States Department of Agriculture, avril 1999. http://dx.doi.org/10.32747/1999.7695836.bard.

Texte intégral
Résumé :
The objectives were to continue collection of semen for the US dairy bull DNA repository, to conduct a systematic search of the Holstein genome for economically significant economic trait loci (ETL), to develop and refine statistical techniques for the analysis of the data generated, and to confirm significant effects by genotyping daughters i Israel and additional US sons. One-thousand-seventy-six sons of eight US grandsires were genotyped for 174 microsatellites located on all 29 autosomes. ETL were detected for milk production traits on seven chromosomes. ETL for milk and fat yield and fat and protein percentage on BTA3 was mapped to between the markers BL41 and TGLA263. The 95% confidence interval for the ETL affecting fat percentage on BTA14 localized this ETL between the contromere and chromosome position 11 cM. This ETL was verified in the Israeli cattle population by genotyping an independent sample of cows from seven families. The radiation hybrid data for the centromeric region of BTA14 is defined by a single linkage group. Order of Type I genes within this region, CYC-FADK-TG-SQLE, is conserved between human and cattle. Thus, HSA8, the human homologue of BTA14, can be used to identify candidate genes for the ETL.
Styles APA, Harvard, Vancouver, ISO, etc.
10

Womack, James E., Moshe (Morris) Soller et Jacques S. Beckmann. Mapping the Dairy Cattle Genome Using Somatic Cell Hybrids and Recombinant DNA Technology. United States Department of Agriculture, septembre 1986. http://dx.doi.org/10.32747/1986.7566850.bard.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
Vous pouvez également être intéressé par les bibliographies sur le sujet « DNA hybrid » pour d’autres types de sources :
Articles de revues Thèses
Nous offrons des réductions sur tous les plans premium pour les auteurs dont les œuvres sont incluses dans des sélections littéraires thématiques. Contactez-nous pour obtenir un code promo unique!

Vers la bibliographie