Articles de revues sur le sujet « Dat 760f »

A seletividade de herbicidas aplicados em pré-emergência depende das características do solo cultivado. Neste contexto, o objetivo deste trabalho foi avaliar a seletividade do herbicida fomesafen à cultura do algodoeiro em solos de classe textural contrastantes. O experimento foi realizado em casa de vegetação no delineamento experimental inteiramente casualizado, com cinco repetições. Os tratamentos foram arranjados em esquema fatorial 5 x 2, com o primeiro nível correspondente a cinco doses de fomesafen (0, 175, 350, 525 e 700 g ha-1) e o segundo fator de dois solos de textura contrastante: textura franco arenosa (200 g kg-1 de argila, 40 g kg-1 de silte e 760g kg-1 de areia, pH de 4,47 e 1,59% de M.O.) e muito argilosa (760 g kg-1 de argila, 110 g kg-1 de silte e 130g kg-1 de areia, pH 5,7 e 2,30% de M.O.). As avaliações realizadas foram: a contagem de plantas emergidas aos 7 e 14 dias após a emergência (DAE), a fitointoxicação realizada aos sete, 14 e 21 DAE. Aos 35 DAE, foi realizada a coleta de parte aérea e das raízes para determinação da massa seca de raízes e de parte aérea. O fomesafen apresentou potencial de uso para o algodoeiro, cultivar FM 993, todavia, os sintomas de fitointoxicação foram muito intensos nas plantas tratadas com as maiores doses no solo de textura franco arenosa. Desta forma, deve-se ajustar a dose de fomesafen de acordo com a classe textural e o teor de matéria orgânica do solo.

ABSTRAK Serasah mangrove yang jatuh di lantai mangrove akan di dekomposisi oleh mikroorganisme heterotrof yang memiliki kemampuan mendegradasi serasah mangrove. Tujuan dari penelitian ini adalah mengetahui kerapatan mangrove, total bakteri heterotrof perairan pada beberapa kerapatan mangrove, Indeks Kualitas Air (IKA) serta hubungan kerapatan mangrove dengan total bakteri heterotrof dan Indeks Kualitas Air (IKA) perairan di Desa Bedono, Demak. Penelitian ini menggunakan metode deskriptif, dengan mengukur parameter pendukung yaitu parameter fisika dan kimia, dan melakukan perhitungan kelimpahan bakteri heterotrof menggunakan metode TPC (Total Plate Count). Hasil pengamatan menunjukan bahwa kelimpahan bakteri heterotrof tinggi berkisar antara (2.120-5.620) x 10-6 Cfu/ml pada saat pasang dan 760-4.840 x 10-6 Cfu/ml pada saat surut.. Berdasarkan hasil penelitian dapat disimpulkan bahwa kerapatan mangrove pada Desa Bedono berkisar antara 900-2.400 pohon/Ha; kelimpahan bakteri hetrotrof di Desa Bedono berkisar antara 760x 10-6 Cfu/ml – 5.620 x 10-6 Cfu/ml; Indeks Kualitas Air (IKA) yang didapatkan berkisar antara 33,1-46,93; terdapat hubungan yang berbanding lurus antara kelimpahan bakteri heterotrof dengan kerapatan mangrove, dan hubungan berbanding terbalik antara Indeks Kualitas Air (IKA), kelimpahan bakteri heterotrof dan kerapatan mangrove. Kata Kunci: Bakteri Heterotrof, Kerapatan Mangrove, Desa Bedono, Indeks Kualitas Air ABSTRACT The mangrove litter that falls on the mangrove floor will be decomposed by heterotrophic microorganisms that have the ability to degrade the mangrove litter. This research is purposed to know the density of mangrove in Bedono, Demak; Looking for total heterotrophic bacteria in some mangrove densities in Bedono village, Demak; knowing Water Quality Index (IKA) in Bedono Village; know the relationship of mangrove density with total heterotrophic bacteria, and Water Quality Index (IKA) in Bedono Village, Demak. This study uses a descriptive method, by measuring the supporting parameters of physical and chemical parameters, and calculating the abundance of heterotrophic bacteria using TPC (Total Plate Count) method. The observed results show the high abundance of heterotrophic bacteria ranging between (2.120-5.620) x 10-6 Cfu/ml at high tide and 760-4.840 x 10-6 Cfu/ml at low tide. Based on the results of the research can be concluded that the mangrove density in Bedono village ranged between 900-2.400 trees/Ha; The Water Quality Index (IKA) that occurs is between 33,1-46,93; There is a direct relationship between the abundance of heterotrophic bacteria with mangrove density, and the inverse relationship between the Water Quality Index (IKA), abundance of heterotrophic bacteria and mangrove density. Keywords: Heterotrophic Bacteria, Mangrove Density, Bedono Village, Water Quality Index

Abstract The fairway consisted primarily of annual bluegrass (40%), Kentucky bluegrass (40%), and perennial ryegrass (20%). Treatment plots were 9 × 6 ft, arranged in a RCB design, replicated 3 times. Liquid formulations were applied with a CO2 compressed air sprayer with 4 8004VS TeeJet nozzles mounted on a 6 ft boom, operating at 28 psi, and delivering 5 gal/1000 ft2. At treatment time (26 Jul) the following soil and environmental conditions existed: air temperature, 72°F; soil temperature at 1 inch depth, 76CF; soil temperature at 2 inch, 74°F; RH, 85%; amount of thatch, 0.25-0.50 inches; soil type, loam; soil particle size analysis: 31.1% sand, 46.4% silt, 22.5% clay; organic matter, 6.7%; soil moisture (oven baked) 23.1%; water pH, 6-7; soil pH, 4.4; and partly cloudy skies. Immediately after treatment the experimental area was irrigated with 0.25 inches of water. Posttreatment counts were made 42 DAT (6 Sep). Three ft2 sod samples were randomly taken from each replicate, and the total number of Japanese beetle grubs ft2 was recorded.

The content and composition of 1,2-diacyl-sn-glycerol (1,2-DAG) was determined in hepatocytes from saline (0.9% NaCl)- and Escherichia coli endotoxin (ET)-infused rats upon continuous vasopressin (VP) (10(-8) M) stimulation. In both experimental groups the accumulation of 1,2-DAG was detected after a lag period (2-5 min), was sustained up to the last time analysed (10 min), and C18:0- and C20:4-fatty-acid-containing-DAG accumulation preceded that of DAG containing other acyl groups. In hepatocytes from ET-infused rats the VP-induced accumulation of DAG was delayed and was decreased by 50%, showing a C18:0/C20:4 molar ratio of 1.6 as compared with 1.1 for cells from saline-infused rats. A similar lower cellular response to VP stimulation was observed in cells prelabelled with [14C]C20:4 fatty acid. The accumulation of [14C]C20:4-DAG (lower in ET than in saline-infused rats) was paralleled by a decrease in phosphatidylinositol (PI) labelling, whereas phosphatidic acid showed a transient increase by 5 min in saline- but not in ET-infused rats. The present results demonstrate that the previously reported impairment in the early degradation of poly-PI and later in the ‘PI cycle’ during VP stimulation [Rodriguez de Turco & Spitzer (1987) Metab. Clin. Exp. 36, 753-760] is also reflected at the level of their phosphodiesteratic product, DAG. Moreover, the kinetics of the accumulation of DAG acyl groups is consistent with the idea that the initial release of C18:0- and C20:4-DAG (possibly derived from inositol lipids) could regulate the subsequent enlargement of this pool by stimulating a phospholipase C-mediated degradation of other phospholipids (e.g. phosphatidylcholine).

Abstract Introduction: Bruton’s tyrosine kinase (BTK), a member of the Tec family, plays a crucial role in signaling through B-cell receptor (BCR). BTK inhibition blocks BCR signals and prevents B-cell activation and growth. First-generation BTK inhibitors such as ibrutinib covalently binds to a cysteine residue (C481) of BTK. Their most frequent acquired resistance is the development of a serine mutation in the binding site (C481S). Next generation BTK inhibitors such as LOXO-305 and ARQ 531 are being developed to overcome this resistance to first-generation inhibitors. Methods: HMPL-760 was tested in biochemical assays using recombinant human wild type (WT) and C481S mutant BTKs. Its selectivity was carried out using Eurofins Cerep KinaseProfilerTM panel. Cellular activity of HMPL-760 was evaluated in HEK293 cells stably transfected with BTKWT or BTKC481S, and other tumor cell lines, which are either human diffuse large B cell lymphoma (DLBCL) or mantle cell lymphoma (MCL) cell lines. The in vivo antitumor activity and PKPD correlation of HMPL-760 was studied in HBL-1 xenograft mouse models bearing BTKWT or BTKC481S respectively. Results: In biochemical assays, HMPL-760 strongly inhibits BTK kinase activities towards wild-type BTK (BTKWT) and C481S mutant (BTKC481S), and binds to BTK in a reversible way. HMPL-760 demonstrates high selectivity in a panel containing 413 kinases. In cellular assays, HMPL-760 displays strong anti-proliferative activities in B-cell lymphoma cells (TMD-8, OCI-LY10, REC-1, HBL-1 and HBL-1-BTKC481S) harboring either BTKWT or BTKC481S (GI50: 0.0015-0.046 μM). In human whole blood assay, HMPL-760 inhibits activation of B-cells at nanomolar concentrations measured by inhibition of immunoglobulin-induced CD69 expression in CD19+cells. HMPL-760 shows ≥ 10-fold inhibitory potency than ARQ 531 in both BTKWT and BTKC481S cells, and ~3-fold higher inhibitory potency than that of LOXO-305 in BTKC481S cells. In cellular assay by detecting p-BTK after compound washout, HMPL-760 maintains a longer duration of target inhibition than LOXO-305 in both BTK wild type (HBL-1) and BTK mutant (HBL-1-BTKC481S) cell lines. HMPL-760 displays dose-dependent antitumor efficacy in multiple human B cell lymphoma xenograft models in mice when orally administered at 3~50 mg/kg once daily. Complete tumor regression occurs in most of the tested models at the high dose levels. HMPL-760 shows much stronger antitumor efficacy than LOXO-305 and ARQ 531 at similar dose level, which may be associated with HMPL-760’s higher drug exposures and more sustainable inhibition on BTK phosphorylation in the tumor tissues. Conclusion: HMPL-760 is a reversible, selective, highly potent, BTK inhibitor targeting both BTKWT and BTKC481S. The first-in-human Phase 1 clinical trials of HMPL-760 are under way in patients with r/r B-NHL (NCT05190068, NCT05176691). Citation Format: Linfang Wang, Junqing Liang, Zhihu Gao, Jia Hu, Weigang He, Xianwen Yang, Fangfang Mao, Wei Zhang, Ying Yu, Qihang Zhang, Na Yang, Chun Zhang, Jian Wang, Yu Cai, Xiong Li, Weiguo Qing, Guangxiu Dai, Yongxin Ren, Michael Shi, Weiguo Su. HMPL-760 is a highly potent and selective reversible BTK inhibitor, targeting BTK and BTKC481S in B-cell malignancies. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 4020.

Dam construction provides economic importance to the environs through, water supply and flood control. Post-construction investigation is however necessary for maintenance stability to avoid irreversible environmental changes. In view of this, horizontal resistivity profiling and vertical electrical sounding (VES) were conducted along the embankments and the downstream of Asejire dam to determine its integrity. The methods used were horizontal resistivity profiling and vertical electrical soundings conducted along the embankments and the downstream. Thirty-one VES and Dipole-Dipole Profiling were occupied along the embankments and downstream using Resistivity meter at 20 m intervals for both the VES and Dipole-Dipole. The Dipole-Dipole data were inverted into 2-D Resistivity Images using DIPPRO™ 4.0 Inversion Software while the VES data were quantitatively interpreted using the partial curve matching technique and Winresist 1.0 Version Software. Results of dipole-dipole image and geo-electric section identified three geo-electric layers; comprising topsoil presumably clayey sand and laterites with resistivity and thickness between 59-760m and 0.7-5.2m respectively. The second layer is weathered layer attributed with clay/clayey sand having resistivity and thickness 18-766m and 3.1-36.7 m. The third layer suspected to be fresh bedrock with resistivity range 121-3672m and 18-766 m thick. The Dipole-Dipole results displayed resistivity less than 500m in the first layer with structural evidence of discontinuous observables, but the underlying sequences displayed no indicative of structural weakness. This significant features play a major role in seepage processes from the dam, therefore lithological composition obtained from these results shows the dam has a good integrity.

The aim of the study is to reconstruct the forest vegetation of the Czech Republic in the period between agricultural prehistory (Neolithic, LnK, c. 7600 BP) and the Early Middle Ages on an elevation gradient from 140 to c. 500 m above sea level using anthracological data from archaeological sites – ARV (anthracologically reconstructed vegetation). A large set of data from 601 archaeological sites (990 components) containing 289,489 identified charcoal fragments was analysed. The analysed set of data contains both a selection of previously published results and, especially, a large amount of as yet unpublished data from the authors of the article. A basic comparison of the ARV with the composition of the potential natural vegetation (PNV after Neuhäuslová et al. 1998) was then conducted. The chronological development of the anthracologically reconstructed vegetation is compared with information from pollen analyses (PRV after Abraham et al. 2016) from the Czech Republic. The results of our study clearly show that anthracological data are an important source of information concerning the forest vegetation on land mostly deforested today, at lowland forest sites heavily affected by erosion and forests at medium elevations. The text outlines the basic ecological characteristics of selected anthracologically distinguishable tree taxa and methodological challenges in anthracological analysis.

Date fruit (Phoenix dactylifera) is rich source of nutrition and is consumed as the healthiest alternative food content from fruit source in Saudi Arabia. Due to its popularity date palm is grown extensively in Ha’il region and many oth-er parts of the country. The study was aimed to evaluate phenolic and flavo-noid contents of selected varieties of date palm (Phoenix dactylifera L.) fruits collected from, Ha’il Saudi Arabia. Total phenolic content of each extract was determined by the Folin-Ciocalteu reagent method and its absorbance was measured at 760 nm. Total flavonoid was extracted in a Soxhlet extractor with ethanol and then absorbance was measured against a blank at 510 nm. Total phenolic content of the investigated varieties ranged from 154 to 39 mg GAE/100g DM. The highest phenolic content was found in Ajwah, Umm-Hamam, Romaithyyah and Berhi respectively, while the lowest content was observed in Shaishi variety. On the other hand, total flavonoid content of the investigated varieties ranged from 28.85 to 95.31mg GAE/100g DM. The highest flavonoid content was found in Umm-Hamam, while the lowest con-tent was observed in Menefi variety. Such variation in phenolic and flavonoid values could be attributed to several parameters, including date variety, stage of fruit picking, storage, postharvest processing, geographical origin of tree and soil condition. However, regardless this variation in phenolic and flavonoid contents, the current study confirms richness of Ha’il date fruits;Ajwah, Umm-Hamam, Romaithyyah and Berhi of these phytoconstitu-ents which are well-documented to possess important medicinal values.

Abstract A study was conducted at the Louisiana State University Agricultural Center’s H. Rouse Caffey Rice Research Station in 2017 and 2018 to evaluate the interaction between a prepackage mixture of clomazone plus pendimethalin applied at 0, 760, 1,145, or 1,540 g ai ha−1 mixed with propanil at 0, 1,120, 2,240, or 4,485 g ai ha−1. A synergistic response occurred when barnyardgrass was treated with all rates of clomazone plus pendimethalin mixed with either rate of propanil evaluated at 56 d after treatment (DAT). Unlike barnyardgrass, an antagonistic response occurred in yellow nutsedge used as a control when treated with 760 and 1,540 g ha−1 of clomazone plus pendimethalin mixed with 1,120 or 22,40 g ha−1 of propanil at 28 DAT; however, 1,145 g ha−1 of clomazone plus pendimethalin mixed with 4,485 g ha−1 of propanil resulted in a neutral interaction. At 28 DAT, rice flatsedge treated with all herbicide mixtures resulted in neutral interactions. The synergism of clomazone plus pendimethalin applied at 1,540 g ha−1 mixed with propanil applied at 2,240 or 4,485 g ha−1 to control barnyardgrass resulted in an increased rough rice yield compared with 760 or 1,145 g ha−1 of clomazone plus pendimethalin mixed with propanil applied at 1,120 or 2,240 g ha−1. These results indicate that if barnyardgrass and rice flatsedge are present in a rice field the prepackage mixture of clomazone plus pendimethalin mixed with propanil can be an option for growers. However, if yellow nutsedge infest the area other herbicides may be needed.

Abstract TBR-760 is a chimeric dopamine (DA)-somatostatin (SST) compound with potent agonist activity at both DA type 2 (D2R; EC50 0.064nM) and SST type 2 (SSTR2; EC50 1.2nM) receptors. Prior studies have demonstrated that the chimeric DA-SST compounds are more potent and effective than either individual or combinations of individual DA and/or SST analogs in inhibiting secretion from pituitary adenomas. Non-functioning pituitary adenomas (NFPA) express high levels of D2R as well as lower levels of SSTRs, including the type 2 receptor (1), and thus have an appropriate receptor profile to respond to TBR-760. The present study examines the ability of TBR-760 to inhibit tumor growth in a mouse model of aggressive NFPA. Heterozygous and null mutant mice lacking one or both copies, respectively, of the pro-opiomelanocortin (POMC) gene (POMC-KO mice)(2) spontaneously develop aggressive, non-secreting pituitary adenomas (3). The POMC-KO mouse tumors have been shown to express D2R and SSTR2 at a similar level as human NFPAs (4). In addition, merging of microarray data (Affymetrix, U133 plus_2.0 and Mouse Genome 430 2.0 arrays), reveals 154 common gene signatures between human NFPAs and the POMC-KO mouse tumors. In an initial study, heterozygous POMC-KO mice with an established pituitary tumor of approx. 10mm3 (mean volume 8.9±0.3), as determined by MRI, were treated with a range of TBR-760 doses (0.125 to 12.5mg/kg, sc, QD) for 60 days. During that time, tumors in vehicle-treated mice increased in size by 890±0.7%, whereas all doses of TBR-760 tested resulted in a nearly complete inhibition of tumor growth from treatment initiation. We then compared the effect of the TBR-760 chimera with that of its individual SST agonist (SSTA) and DA agonist (DAA) components on tumor growth in the POMC-KO mice. As in the earlier study, TBR-760 treatment (1mg/kg, sc, QD), initiated when the mice had an established tumor of approx. 10mm3, completely arrested tumor growth during the 8 weeks of treatment (final mean tumor volume of 8.5±1.3mm3 vs. 54.61±10.6mm3 in vehicle-treated mice). Treatment with equimolar or 10x-higher doses of the individual SSTA or DAA, either alone or in combination, had no significant effect on tumor growth, except in the lower dose DAA group where a modest suppression of tumor growth was observed. These data demonstrate that only the dual DA-SST chimeric compound, TBR-760, completely arrested tumor growth in the POMC-KO mouse model of NFPA. Further, despite the highly aggressive nature of the POMC-KO tumors, significant tumor shrinkage was observed in 20% of the mice treated with TBR-760. These results support the development of TBR-760 as a medical therapy to prevent or arrest the growth of NFPAs and, potentially, to induce NFPA shrinkage. References: (1) Florio et al., 2008 Endocr Relat Cancer; 15: 583-596. (2) Yaswen et al., 1999 Nat Med; 9:1066-70 (3) Karpac J et al., 2006 Cell Mol Biol; 52: 47-52.

Leipzig, 08. Oktober 2020 Das diesjährige Motto der 36., aufgrund der Corona-Situation erstmals „hybriden“ Jahrestagung der Deutschen Adipositas-Gesellschaft (DAG) spannt einen weiten inhaltlichen Bogen von der Prävention über die ursächlichen Mechanismen auf zellulärer und Stoffwechselebene bis hin zu einer effektiven Therapie und bezieht auch Stigmatisierung sowie weitere soziokulturelle, psychologische, gesellschaftliche und globale Aspekte der Adipositas (schweres Übergewicht) mit ein. Ein aktuelles Thema ist die Auswirkung der Covid-19-Epidemie auf Menschen mit Adipositas 1, 2. Zur Kongresseröffnung fordert die DAG in einem offenen Brief an die Ständige Impfkommission am Robert Koch-Institut (STIKO) eine prioritäre Covid-19-Impfung für Menschen mit Adipositas 3.

Diffuse alveolar hemorrhage (DAH) is a life-threatening complication of hematopoietic cellular therapy (HCT). This study aimed to evaluate the effect of DAH treatments on outcomes using data from consecutive HCT patients clinically diagnosed with DAH from 3 institutions between January 2018-August 2022. Endpoints included sustained complete response (sCR) defined as bleeding cessation without recurrent bleeding, and non-relapse mortality (NRM). Forty children developed DAH at a median of 56.5 days post-HCT (range 1-760). Thirty-five (88%) had at least one concurrent endothelial disorder, including transplant-associated thrombotic microangiopathy (n=30), sinusoidal obstructive syndrome (n=19), or acute graft versus host disease (n=10). Fifty percent had a concurrent pulmonary infection at the time of DAH. Common treatments included steroids (n=17, 25% sCR), inhaled tranexamic acid (INH TXA,n=26, 48% sCR), and inhaled recombinant activated factor VII (INH fVIIa, n=10, 73% sCR). NRM was 56% 100 days after first pulmonary bleed and 70% at 1 year. Steroid treatment was associated with increased risk of NRM (HR 2.25 95% CI 1.07-4.71, p=0.03), while treatment with INH TXA (HR 0.43, 95% CI 0.19- 0.96, p=0.04) and INH fVIIa (HR 0.22, 95% CI 0.07-0.62, p=0.005) were associated with decreased risk of NRM. Prospective studies are warranted to validate these findings.

In this study, the concentration, type, size, and color of MPs in multiple environmental compartments was investigated in Sürgü Dam Reservoir. The MP concentrations in surface water were between 106.63 and 200 par.m-3. The MP concentrations in sediment were between 760 and 1.440 par.m-2. A total of 44 MPs, ranging from 0 to 3 samples per fish with averaging 0.41 MPs/individual, were extracted from gastrointestinal tracts of fish. Fibers were the predominant type of MPs in surface water, sediment and fish. The most common MP sizes were 1-2 mm in surface water, 0.2-1 mm in sediment and in fish. The dominant color of detected MPs was black in surface water and transparent in sediment and fish. Polyethylene terephthalate and polypropylene were the major polymer types of the selected particles. Of the two stations, station 1 showed a higher MP concentration level. The results of this study showed the MP concentration in SDR is relatively moderate in sediment although it is lower in fish and surface water samples. This data may assist in extending our knowledge regarding MPs pollution in freshwater systems and provides a baseline for future monitoring and assessment MPs of SDR.

Abstract Background Australia is committed to eliminating the hepatitis C virus (HCV) by 2030. Despite regulations in Australia that enable the prescription of subsidised direct acting antiviral (DAA) by primary health care providers, the number of providers who treat patients for HCV remains low and this limits the prospect of HCV elimination. The Prince Charles Hospital, Brisbane, Australia, implemented an innovative program called Cure-It aimed at engaging primary care providers in community-based HCV treatment. This paper aims to describe initial experiences and short-term patient outcomes of this program. Methods A formative evaluation was conducted using program data for the period March 2016 to April 2018. Descriptive statistics were used to report the number of engaged primary care providers, patients’ baseline characteristics, treatment plans, and treatment outcomes. Results Thirty primary care providers from different settings were engaged in HCV treatment. Among 331 patients eligible for community-based treatment, 315 (95.2%) commenced treatment, the completion rate was 92.4 and 66.5% achieved sustained virological response at 12 weeks (SVR12). The SVR12 had not been documented for 26.8% of patients. Among patients whose SVR12 was documented, 98.2% achieved SVR12. Only 1.3% of patients experienced treatment failure. Conclusion A flexible tertiary-led model can improve primary care providers and patients’ engagement with provision of HCV treatment. Tertiary centres need to play their role to improve the accessibility of HCV treatment through providing training and on-going support for primary care providers while enabling those providers to become more confident in providing treatment independently.

We aimed to demonstrate that breath analysis can be used as a method for the detection of potentially harmful compounds in food after their ingestion. Development of such a method could be adapted as a tool for detection of food intoxication. To achieve this, we compared the levels of pyridine (Py) and furfuryl alcohol (FFA) found in breath with the quantity of these compounds ingested when drinking coffee. Coffee drink beverages were prepared in the laboratory and consumed by volunteers (n = 5). An aliquot of coffee was analysed using high performance liquid chromatography with diode-array detection (HPLC-DAD) to quantify Py and FFA in the beverage. Breath samples were collected several times over a 45 min period after ingestion of coffee and analysed by thermal desorption coupled to gas chromatography/mass spectrometry (TD/GC-MS). The levels of Py and FFA found in coffee ranged from 0.2 to 3 mg/cup of coffee, and from 7 to 30 mg/cup of coffee, respectively. The levels of these compounds detected in breath ranged from 7 to 1200 ng/l breath for Py and from 1 to 760 ng/l breath for FFA. Several parameters can influence the levels of these chemicals in breath, especially the collection time of the breath sample.

We aimed to demonstrate that breath analysis can be used as a method for the detection of potentially harmful compounds in food after their ingestion. Development of such a method could be adapted as a tool for detection of food intoxication. To achieve this, we compared the levels of pyridine (Py) and furfuryl alcohol (FFA) found in breath with the quantity of these compounds ingested when drinking coffee. Coffee drink beverages were prepared in the laboratory and consumed by volunteers (n = 5). An aliquot of coffee was analysed using high performance liquid chromatography with diode-array detection (HPLC-DAD) to quantify Py and FFA in the beverage. Breath samples were collected several times over a 45 min period after ingestion of coffee and analysed by thermal desorption coupled to gas chromatography/mass spectrometry (TD/GC-MS). The levels of Py and FFA found in coffee ranged from 0.2 to 3 mg/cup of coffee, and from 7 to 30 mg/cup of coffee, respectively. The levels of these compounds detected in breath ranged from 7 to 1200 ng/l breath for Py and from 1 to 760 ng/l breath for FFA. Several parameters can influence the levels of these chemicals in breath, especially the collection time of the breath sample.

Introdução: As plantas medicinais são capazes de fornecer fármacos de grande importância clínica, a exemplo dos monoterpenos. Estes são conhecidos por apresentarem uma variedade de efeitos em diferentes sistemas biológicos, justificando a necessidade de pesquisas para investigação do potencial terapêutico e tóxico. Com o avanço tecnológico, modelos in silico vêm sendo amplamente aplicados para a avaliação de potenciais atividades farmacológicas e de toxicidade de compostos em ambientes metabólicos de mamíferos. Objetivo: Avaliar os efeitos farmacológicos e toxicológicos do monoterpeno Neral com finalidade odontológica, utilizando uma metodologia in silico. Metodologia: Inicialmente utilizou-se o software Pubchem® para o desenho da molécula, em seguida a análise da probabilidade da atividade da molécula foi realizada com o software Pass Online®. Na análise dos parâmetros farmacológicos, foi avaliado a biodisponibilidade oral teórica do Neral, pela “Regra dos Cinco” de Lipinski com o software Molinspiration Cheminformatics. Finalmente, os parâmetros toxicológicos bem como o estudo teórico sobre o efeito carcinogênico, o teste de AMES e a toxicidade oral aguda foi efetuada no programa admetSAR. Resultados e Conclusão: No Pass Online a molécula do Neral possui 14 possíveis atividades farmacológicas relacionadas à Odontologia, dentre elas potencial antifúngico, anti-inflamatória e antibacteriana; no Molinspiration a molécula do Neral demonstrou estar de acordo com as cinco regras propostas por Lipinsk, logo, apresentando boa biodisponibilidade oral teórica e, pelo teste de toxicidade do admetSAR, apesar de apresentar baixo risco de toxicidade teórica oral, revelou leve potencial carcinogênico.Descritores: Disponibilidade Biológica; Toxicidade; Plantas Medicinais; Odontologia.ReferênciasLima GR. 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