Thèses : « DAC Report »

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Darpy, Denis. « La procrastination du consommateur : une contribution à l'explication du report d'achat ». Paris 9, 1999. https://portail.bu.dauphine.fr/fileviewer/index.php?doc=1999PA090019.

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Quilice, Thiago Ferreira. « Aspectos positivos e negativos no modelo de reporte proposto pela GRI : a opinião das organizações que reportam ». Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/96/96132/tde-18092014-111311/.

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A prática de divulgar relatórios de sustentabilidade tem se tornando comum em organizações por todo o mundo; em especial no Brasil, há uma forte tendência de crescimento na adoção desta prática. O objetivo deste trabalho foi identificar porque as organizações reportam e os aspectos positivos e negativos no modelo de reporte de sustentabilidade proposto pela Global Reporting Initiative (GRI), na opinião dos funcionários apontados nos relatórios de sustentabilidade como responsáveis por eles; estas informações são importantes porque poucas pesquisas investigam os relatórios de sustentabilidade a partir deste ponto de vista e porque oferece à GRI pontos específicos a serem melhorados. A pesquisa foi realizada por meio da análise de conteúdo, sem a utilização de softwares específicos para o método, das respostas dos funcionários de 27 empresas às questões: Por que elaborar relatório de sustentabilidade? Quais aspectos positivos você identifica no modelo de reporte proposto pela GRI? e quais os negativos? Como resultado, foram obtidas sete categorias sobre por que as organizações emitem relatório de sustentabilidade: apoiar a gestão, acompanhar o mercado, prestar contas, contribuir com outras empresas, abertura do capital, relacionamento com stakeholders e melhorar a imagem da empresa. As categorias de aspectos positivos identificadas foram: melhora a gestão da empresa, proporciona uma ferramenta de marketing, o guia proposto para a elaboração do relatório, possibilidade de legitimação e auxilia no benchmarking. E as categorias de aspectos negativos: dificuldades no processo proposto, dificuldades internas, modelo do relatório final, avaliação dos relatórios, visão externa dos relatórios, indicadores, utilização do relatório como ferramenta de marketing e foco em grandes empresas. Como conclusão, verificou-se que as empresas encontram nas diretrizes da GRI mais do que um modelo de reporte, chegando a representar uma ferramenta de gestão da sustentabilidade; porém, os aspectos negativos identificados colocam em risco a eficácia do projeto de reporte, principalmente devido ao excesso de flexibilidade dos relatórios, o que permite a emissão de diversos formatos de relatórios, muitos dos quais pouco objetivos e com chance de conter informações não confiáveis. Sugerem-se então algumas propostas de melhorias, entre elas a definição de um quadro-resumo que forneça ao leitor uma visão global do desempenho da organização em sustentabilidade, a definição de um modelo específico de relatório final e o desenvolvimento de um novo padrão de classificação dos relatórios.
The practice of disclosing sustainability reporting is becoming common in organizations throughout the world; especially in Brazil, there is a strong upward trend in the adoption of this practice. The objective of this study was to identify positive and negative aspects in the framework for sustainability reporting proposed by the Global Reporting Initiative (GRI), according to the opinion of the staff responsible for the report; this information is important because few studies investigate the sustainability reports from this point of view and because it offers to GRI specific points to be improved. Held data collection by means of open questions that evaluated: why report sustainability reports; positive aspects in the guidelines; and negative aspects of the proposed guidelines. The investigation of responses obtained was through content analysis, using an exploratory qualitative approach. As a result, there are seven categories on why organizations issue a sustainability report: management support, market monitoring, accountability, contribute to other companies, going public, stakeholder relations and improve the company\'s image. The categories of positive aspects are: improved management of the company, provides a marketing tool, the proposed method for preparing the report tab, possibility of legitimation and helps in benchmarking. And the categories of negative aspects: difficulties in the proposed process, internal difficulties, model of the final report, evaluation reports, external view of the reports, indicators, use the report as a marketing tool and focus on large companies. In conclusion, it was found that companies encounter on the GRI guidelines more than one reporting model, came to represent a management tool for sustainability; However, the negative aspects identified jeopardize the effectiveness of the project reporting, mainly due to excessive flexibility of reporting, which allows the issuance of various types of reports, many of which little goals and likely to contain unreliable information. Then are suggested some proposals for improvements, including propose a summary table that provides the reader with an overview of the organization\'s performance in sustainability, propose a specific model of the final report and develop a new standard for classification od reports.

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Avila, Rosa M. « The Getting Ready to Learn Program : An Impact Report ». [Tampa, Fla] : University of South Florida, 2008. http://purl.fcla.edu/usf/dc/et/SFE0002405.

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Hester, Jessica Green. « A report on an internship with the National D-Day Museum, New Orleans, Louisiana ». ScholarWorks@UNO, 2002. http://scholarworks.uno.edu/aa_rpts/19.

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From May 28, 2002 to August 20, 2002, I served as an intern in the collections department at The National D-Day Museum in New Orleans, Louisiana. The National D-Day Museum (NDDM) is a 501(c) (3) history museum that specializes in the amphibious invasions of World War II. The institute is very new, opening in 2000, and has experienced an unpredictable amount of success and acclaim. This rapid success and growth has created unique challenges for the organization as it tries to develop. The following paper is broken into five chapters and a conclusion. Chapter 1 is an introduction to NDDM, including its history, mission, organizational structure, funding and programs. Chapter 2 is a description on my internship that includes tasks and responsibilities. Chapter 3 discusses the major problem that the collections department faces (lack of staff) and Chapter 4 gives a recommendation on how to solve this problem. Chapter S discusses my effect on the organization and the paper ends in a conclusion of the organization and my experiences.

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Blagrove, Mark Thomas. « The narrative of dream reports ». Thesis, Brunel University, 1989. http://bura.brunel.ac.uk/handle/2438/5147.

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Two questions are addressed: 1) whether a dream is meaningful as a whole, or whether the scenes are separate and unconnected, and 2) whether dream images are an epiphenomenon of a functional physiologicaL process of REM sleep, or whether they are akin to waking thought. Theories of REM sleep as a period of information-processing are reviewed. This is Linked with work on the relationship between dreaming and creativity, and between memory and imagery. Because of the persuasive evidence that REM sleep is implicated in the consolidation of memories there is a review of recent work on neural associative network models of memory. Two theories of dreams based on these models are described, and predictions with regard to the above two questions are made. Psychological evidence of relevance to the neural network theories is extensively reviewed. These predictions are compared with those of the recent application of structuralism to the study of dreams, which is an extension from its usual field of mythology and anthropology. The different theories are tested against four nights of dreams recorded in a sleep Lab. The analysis shows that not only do dreams concretise waking concerns as metaphors but that these concerns are depicted in oppositional terms, such as, for example, inside/outside or revolving/static. These oppositions are then permuted from one dream to the next until a resolution of the initial concern is achieved at the end of the night. An account of the use of the single case-study methodology in psychology is given, in addition to a replication of the analysis of one night's dreams by five independent judges. There is an examination of objections to the structuralist methodology, and of objections to the paradigm of multiple dream awakenings. The conclusion is drawn that dreams involve the unconscious dialectical step-by-step resolution of conflicts which to a great extent are consciously known to the subject. The similarity of dreams to day-dreams is explored, with the conclusion that the content of dreams is better explained by an account of metaphors we use when awake and by our daily concerns, than by reference to the physiology of REM sleep. It is emphasised that dreams can be meaningful even if they do not have a function.

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Zinchenko, Yulia. « IBM Cognos Report Studio as an Effective Tool for Human Capital Reporting ». Master's thesis, Vysoká škola ekonomická v Praze, 2013. http://www.nusl.cz/ntk/nusl-199724.

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Main topic discussed in this diploma thesis is corporate reporting in terms of Human Capital using Business Intelligence tools, specifically IBM Cognos Report Studio. One of the objectives is to show step-by-step methodology of creating complex dynamic report, which includes data structure modeling, layout design and quality check. Another objective is to conduct Cost-Benefit Analysis for a real-life project, which is focused on recreating of Excel-based report in Cognos-based environment in order to automate information flows. Essential part of the diploma thesis is theoretical background of Business Intelligence aspects of data quality and visualization as well as purposes of human capital reporting and description of appropriate KPIs. Objectives are addressed by conducting analysis and research of resources related to topics described above as well as using IBM Cognos Report Studio provided by one of the major companies in financial advisory field. This diploma thesis represents relevant reading for those, who are interested in real-life application of data quality improvement and information flow automation using Business Intelligence reporting tools.

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Wolfgeher, Stephane L. « Inflation of USAF officer performance reports analyzing the organizational environment ». Thesis, Monterey, California : Naval Postgraduate School, 2009. http://edocs.nps.edu/npspubs/scholarly/theses/2009/Dec/09Dec%5FWolfgeher.pdf.

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Thesis (M.S. in Information Operations)--Naval Postgraduate School, December 2009.
Thesis Advisor(s): Jansen, Erik. Second Reader: Greenshields, Brian. "December 2009." Description based on title screen as viewed on January 27, 2010. Author(s) subject terms: Evaluation, Inflation, Officer performance report, Officer evaluation system, OPR, OER, Open system, Congruence in organizations, Organizational change, Military reward systems. Includes bibliographical references (p. 83-89). Also available in print.

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Santos, Hélder Guimarães de Oliveira. « Portuguese sell-side analysts' reports : can they deliver ? » Master's thesis, Universidade de Aveiro, 2011. http://hdl.handle.net/10773/7878.

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Mestrado em Gestão
This paper studies the importance of Portuguese financial sell-side analysts’ reports by testing reliability in firms’ Price Target calculation and information aptitude (deliver ability) in the content of sell-side analysts’ reports. The importance of sell-side analysts reports has long been studied, mainly in two different perspectives: the consequences of their work (market price reactions, trading strategies based in analysts’ recommendations) and the externalizations that influence their work (herding and bias behaviors). We believe that before either perspective can explain their value, analysts through their reports should be able to deliver the information users need and offer coherent calculation that justifies the Price Targets. We explore and encode the complete content of 73 reports from PSI20 listed companies, and apply consistency and reliability procedures to test them. Informativeness is tested against an ideal report (built mainly from the Jenkins Report conclusions). To test reliability in the Prices Targets calculations we investigate if the method and the parameters of the evaluation are clearly disclosed and if the calculative procedure is according to the theoretical conventions.
O presente trabalho propõe-se avaliar a importância dos analistas financeiros Portugueses testando para isso a fiabilidade no cálculo dos Price Targets e a capacidade informativa dos relatórios que produzem. A utilidade dos analistas financeiros tem sido há muito estudada, por norma através de duas perspectivas: avaliando as consequências do seu trabalho (reacção dos mercados às suas recomendações e estratégias de investimento baseadas nessas mesmas recomendações) e por outro lado considerando as variáveis exógenas que influenciam o seu trabalho (comportamentos tendenciosos e de “arrebanhamento”). Acreditamos que antes de avaliar a pertinência destas perspectivas, importa averiguar se através dos relatórios que produzem os analistas financeiros fornecem a informação que os seus utilizadores necessitam. Para isso examinamos e codificamos 73 relatórios financeiros de empresas que integram o PSI20, testando-os em termos de informatividade e fiabilidade. A capacidade informativa é testada em confronto com um relatório ideal (baseado nas conclusões do Relatório Jenkins). Para testar a confiabilidade no cálculo dos Price Targets investigamos se o método e os parâmetros utilizados são expressos com clareza e se o processo de cálculo está em conformidade com aquilo que são os princípios teóricos aceites.

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Gentile, Eduardo. « Representations of the human rights of brazilian women in a 1977 OAS Report ». Florianópolis, SC, 2005. http://repositorio.ufsc.br/handle/123456789/102060.

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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão. Programa de Pós-Graduação em Letras/Inglês e Literatura Correspondente.
Made available in DSpace on 2013-07-15T23:56:00Z (GMT). No. of bitstreams: 1 221526.pdf: 836562 bytes, checksum: ca5bd58da5a45846e6451912f0a8f717 (MD5)
Partindo de uma perspectiva lingüística de análise do discurso, esta pesquisa investiga a situação dos direitos humanos no Brasil, focalizando os direitos da mulher, em relação à discriminação de gênero e à violência doméstica. Documentos descritivos e normativos do assunto foram publicados por instituições governamentais internacionais e nacionais e também por instituições não-governamentais como movimentos feministas. Entretanto, poucos estudos foram realizados para investigar esses documentos e desvendar as realidades representadas. Considerando o exposto, analiso um dos capítulos do 'Relatório da Situação dos Direitos Humanos no Brasil', elaborado em 1997 pela OEA, intitulado 'Os Direitos Humanos da Mulher no Brasil'. Desenvolvo esta pesquisa baseando meus objetivos na perspectiva do discurso de Fairclough e de Halliday, na qual as identidades e hierarquias sociais são criadas e mantidas através do discurso entre os membros da sociedade. Utilizo a teoria e método da Lingüística Sistêmica Funcional de Halliday para analisar os processos de transitividade que ocorrem no objeto de estudo, para obter as respostas às perguntas da pesquisa. Com este procedimento, a análise revela que as mulheres são representadas como vítimas das violações aos seus direitos humanos, assim como responsáveis por algumas dessas violações e, ao mesmo tempo, ocupam uma posição de submissão na sociedade. Esta pesquisa representa uma contribuição na busca de soluções que possam ajudar a atenuar o problema.

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Hart, Timothy C. « Respondent fatigue in self-report victim surveys : examining a source of nonsampling error from three perspectives ». [Tampa, Fla] : University of South Florida, 2006. http://purl.fcla.edu/usf/dc/et/SFE0001456.

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Xavier, Alexandre Delfino. « Estudo prosódico das disfluências de reparo ». Universidade Federal de Minas Gerais, 2009. http://hdl.handle.net/1843/ALDR-7R6GLS.

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The aim of this dissertation is to describe the prosodic features present in spontaneous speech containing repair disfluências and compare which of these features can diferentiate from the fluent speech. Repair disfluencies strings of sentences in which the speaker corrects himself just after noticing the production of a wrong information. Although they are often considered only as an error during the linguistic processing, the disfluencies can work as an evidence for the production and comprehension language systems. A major part of studies on disfluencies is concerned to aspects as syntax and semantics. The proposal of this work follows a group of investigations that analise the influence of phonological aspects of the disfluencies for the linguist processing models (Ferreira, 2005; Fodor, 1998, 2002; Levelt, 1989, Shriberg, 1994, 1999; among others). Starting from the self-monitoring model and from the remarks on the prosodic marking in repair disfluencies described by Levelt (1983, 1989), we evaluated acoustic features that may characterize a disfluent speech. We used as a basis for the repair corpus the larger previous corpus from Magalhães (2000) about the mineiro accent from Belo Horizonte. The first feature to be analised was the pitch contour, especially the part correspondent to the wrong information (reparandum) e to the corrected information (repair). Furthermore, we analised the behaviour of the F0 peak during the two parts. After that, we analised the issue on the picht contour interruption as an acoustic evidence for the end of the reparandum. The second feature was the intensity values; as the F0 analysis, we compared the value of intensity during the two parts. The third feature was the speech rate, comparing the elocution rate and the articulation rate of speech. We analised the occurence of silent pauses and filled pauses too. Last, we studied the presence of other features, such as lengthening, laryngelizations and creaky voice. Indeed, the results of the acoustic and the statistical analysis point towards to our original hypothesis that the speaker produces significant acoustic cues that may show the difference between the reparandum and the repair. Considering the F0 and the intensity, there is a contrastive accent marking, as a way of highlighting or emphatizing the repair information. The pitch contour interruption is seen as a result of the self-monitoring error detection. The lengthening and the pauses may occur when the speaker needs to plan more the upcoming message as he speaks. The results from this dissertation corroborate the ideas that prosody is an relevant component in the phrasal processing and that the speaker has a self-monitoring speech mecanism.
Este trabalho tem o objetivo de descrever aspectos prosódicos presentes na fala espontânea contendo disfluências de reparo e comparar quais desses aspectos se diferenciam da fala considerada fluente. Disfluências de reparo são trechos de frases em que o falante se corrige após detectar a produção de uma informação errada. Consideradas, muitas vezes, simplesmente como erros ocorridos durante o processamento lingüístico, as disfluências podem servir como evidência do funcionamento dos sistemas de produção e compreensão da linguagem. A maioria dos estudos sobre disfluência considera apenas aspectos sintáticos e semânticos do processamento. A proposta deste trabalho segue um grupo de estudos que analisam a influência de aspectos fonológicos das disfluências nos modelos de processamento lingüístico (Ferreira, 2005; Fodor, 1998, 2002; Levelt, 1989, Shriberg, 1994, 1999, entre outros). Partindo do modelo de auto-monitoramento e das observações sobre a marcação prosódica de disfluências de reparo descritas por Levelt (1983, 1989), avaliamos parâmetros acústicos que caracterizam a fala disfluente. Utilizamos como base de formação do corpus de disfluências o trabalho de Magalhães (2000) sobre o dialeto mineiro de Belo Horizonte. O primeiro parâmetro analisado foi o contorno melódico, sobretudo no trecho correspondente à informação errada (reparandum) e à informação corrigida (reparo). Analisamos, ainda, o comportamento do pico de F0 nos dois trechos. Em seguida, analisamos a questão da ruptura abrupta do contorno melódico como evidência acústica do fim do reparandum. O segundo parâmetro foi a intensidade: como na análise de F0, comparamos os valores de intensidade nos dois trechos. O terceiro parâmetro foi a velocidade de fala, medida pelas taxas de elocução e de articulação. Analisamos ainda a ocorrência de pausas silenciosas e pausas preenchidas. Por fim, a presença de outras características, como alongamentos, laringealizações e creaky voice foram também analisadas. Os resultados de análise acústica e estatística indicam que, de fato, o falante produz pistas acústicas significativas que possam evidenciar uma diferença entre o reparandum e o reparo. Em relação à F0 e à intensidade, existe uma marcação acentual contrastiva, de forma a destacar ou enfatizar a informação do reparo. A interrupção do contorno melódico é resultante da detecção do erro por parte do sistema de auto-monitoramento de detecção de erros. Os alongamentos e as pausas ocorrem quando o falante necessita planejar mais a mensagem à frente enquanto fala. Os resultados deste trabalho corroboram as idéias de que a prosódia é um importante componente no processamento frasal e de que o falante dispõe de um mecanismo de monitoramento da fala.

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Reed, Kristin Bodenhamer-Davis Eugenia. « Evaluation of the Quick Inventory of Depressive Symptomatology-Self Report (QIDS-SR) in a spinal cord injury population ». [Denton, Tex.] : University of North Texas, 2008. http://digital.library.unt.edu/permalink/meta-dc-9045.

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Gradella, Enrico <1992&gt. « Dal bilancio sociale al report integrato : confronto tra documenti di imprese di dimensioni differenti ». Master's Degree Thesis, Università Ca' Foscari Venezia, 2017. http://hdl.handle.net/10579/11753.

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In tale elaborato si è cercato di colpire l'attenzione del lettore con un tema alquanto recente, ossia la reportistica sostenibile. Iniziando dalla letteratura del bilancio sociale o di sostenibilità si sono determinate le variabili fondanti la fattispecie con i relativi esempi, i quali vengono rappresentati da aziende ed organizzazioni di diversa dimensione. Il viaggio nella sostenibilità continua poi con l'evoluzione del sustainability report: il report integrato. Con tale documento si intende presentare una mole informativa agli stakeholder che preveda la concomitanza in un unico report di informazioni finanziare e informazioni non finanziarie, in modo tale da creare una raffigurazione olistica e dinamica della dimensione aziendale. Anche in tal caso, dopo aver presentato la dottrina e i maggiori attori in tema, si portano a visione esemplificazioni di organizzazioni che adottano tale tipologia di reportistica, in modo tale da creare valore nel lungo termine a braccetto con i propri stakholder.

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Lembo, Sara. « The 1996 UK ARbitration Act and the UNCITRAL Model Law : a contemporary analysis ». Doctoral thesis, Luiss Guido Carli, 2010. http://hdl.handle.net/11385/200848.

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Arbitration as an alternative method of settling dispute. The development of Arbitration Law in England. The Arbitration Act 1996 versus the UNCITRAL Model Law: an objective and comparative analysis. Recent trends in International Commercial Arbitration: an empirical analysis.

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Kaczmarski, Jenna M. « Exploring the effects of BMI health report card letters among 6th grade students and parents : an application of the social cognitive theory ». [Tampa, Fla] : University of South Florida, 2009. http://purl.fcla.edu/usf/dc/et/SFE0003198.

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Herzig, Katharina, et Almut Ketzer-Nöltge. « Unterrichtsplanungsmodelle für DaFZ aus interdisziplinärer Perspektive : (Teil I) ». Erich-Schmidt-Verlag, 2019. https://ul.qucosa.de/id/qucosa%3A72168.

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Unterrichtsplanung gehört zu den zentralen Aufgaben für Lehrende. Insbesondere in den Bereichen DaZ und DaF (sowie DaM) müssen sie sich dabei neuen Herausforderungen stellen, was auch Auswirkungen auf die Lehrerendenausbildung hat. So muss sich einerseits das Fach DaFZ vermehrt mit Unterricht im schulischen Kontext beschäftigen, während andererseits DaM-Lehrende zunehmend Kinder mit DaZ-und DaM-Hintergrund gemeinsam unterrichten müssen(vgl. Geist/Krafft 2017;Siebert-Ott 2010). Die Ausbildung der Lehrenden für einen solchen Deutschunterricht an Regelschulen wird häufig von universitären DaF/DaZ-Abteilungen und -Instituten geleistet. Auch in DaF-Kontexten wird der Blick für die heterogenen Voraussetzungen, Bedürfnisse und Lernkontexte immer mehr geschärft und Zielgruppen-und Standortspezifika spielen eine zunehmend wichtige Rolle (vgl. Martinez 2016; Rösler 2016; Riemer 2015; Herzig u. a.2015). Das wirkt sich unmittelbar auf die Planungsaufgaben Lehrender aus: sie werden komplexer, vielfältiger und erfordern flexible Gestaltungsweisen und Lösungen. Insofern scheint es angebracht, aus dem Fach DaFZ heraus angebotene Planungshilfen und die zugrundeliegenden Planungsmodelle und -theorien auf ihre Passgenauigkeit für solche heterogenen Zielgruppen und unterschiedlichen institutionellen Kontexte zu prüfen.

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Hunt, Megan Elaine Yoder Kevin Allan. « Socioeconomic variables associated with the reports of controlling behaviors in current relationships among abused and non-abused females ». [Denton, Tex.] : University of North Texas, 2007. http://digital.library.unt.edu/permalink/meta-dc-5164.

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Ehren, Rico, Ann-Kathrin Kobelt et Annegret Middeke. « Deutsch für Geflüchtete von Anfang an : DaF-/DaZ-Angebote für Geflüchtete im Spannungsfeld von Theorie und Praxis, Institution und Ehrenamt : Dokumentation des 2. Fachtags ». Technische Universität Dresden, 2019. https://tud.qucosa.de/id/qucosa%3A33671.

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Nach einem ersten Fachtag im Jahr 2016, auf dem ehrenamtliche Deutschvermittlungspraxen sichtbar gemacht und Theorie und Praxis in einen Dialog gebracht wurden, ging der Austausch um die den Spracherwerb und die Vermittlung der Sprache von Anfang an betreffenden Herausforderungen in eine neue Phase. Hierbei galt, miteinander zu sprechen, einander zuzuhören statt übereinander zu urteilen. In gemeinsamer Trägerschaft mit dem Netzwerk Dresden für Alle (DfA), mit Unterstützung des Fachverbandes Deutsch als Fremd- und Zweitsprache (fadaf e.V.) und in Zusammenarbeit mit weiteren Kooperationspartnern fand im Herbst 2017, initiiert, organisiert und dokumentiert vom Zentrum für Integrationsstudien der TU Dresden (ZfI), der zweite Fachtag statt. Die vorliegende Dokumentation lässt den wiederholt aufgenommenen Dialog und Austausch sowie die gemeinsame Themen- und Lösungsfindung Revue passieren, fasst dessen zentrale Ergebnisse zusammen und benennt offene Fragen, die weiterhin in einem Dialog auf Augenhöhe zwischen den Akteuren zu diskutieren sind.:Vorwort ... 5 Grußworte zum Fachtag Petra Köpping, Sächsische Staatsministerin für Gleichstellung und Integration ... 7 Geert Mackenroth, Der Sächsische Ausländerbeauftragte ... 9 Eva-Maria Stange, Sächsische Staatsministerin für Wissenschaft und Kunst ... 11 Kristina Winkler, Integrations- und Ausländerbeauftragte der Landeshauptstadt Dresden ... 13 ANN-KATHRIN KOBELT Einleitung ... 15 ANN-KATHRIN KOBELT UND RICO EHREN Dialoge heterogener Perspektiven auf Augenhöhe ... 17 Das Eröffnungspanel ANN-KATHRIN KOBELT Zum Eröffnungspanel ... 21 MICHAEL DOBSTADT UND ANN-KATHRIN KOBELT Austausch, Unterstützung, Selbst- und Prozessreflexion ... 22 ELISABETH SCHMIDT Systemfehler? ... 27 JENS JANSEN Neue Anforderungen an das Ehrenamt ... 29 OLESIA LUKASHEVICH Mein Deutscherwerb von Anfang an ... 30 MARCUS OERTEL Die Perspektive der Kommune ... 32 ANN-KATHRIN KOBELT Das Plenum kommt zu Wort ... 34 Die Workshopphase Die Workshops des Fachtages im Überblick ... 39 Workshop 1 SOPHIA RÖDER UND REBACCA ZABEL Zuschreibungen, Kategorisierungen und Rollenverständnisse in Angeboten sprachlicher Bildung (auch) für Geflüchtete ... 41 Workshop 2 CLAUDIA-E. OECHEL-METZNER UND AHMAD MUHEBBI Alphabetisierung von Erwachsenen in der Zweitsprache Deutsch ... 45 Workshop 3 JANICE BIEBAS-RICHTER, SOLVEIG BUDER UND MICHAEL KOBEL Herausforderungen und Lösungsansätze auf dem Weg in die Ausbildung und Arbeit ... 48 Workshop 4 STEFANIE STUDNITZ UND DOMINIC BÖCKLING Deutsch-Lernmaterialien in der ehrenamtlichen Sprachbegleitung ... 50 Workshop 5 UTE KATJA ENDERLEIN UND KRISTIN KOSSATZ Regelangebote Spracherwerb für Personen mit Migrationshintergrund – Prozesse, Akteure und Anknüpfungspunkte für Ehrenamtliche ... 53 Workshop 6 ANJA CENTENO GARCÍA UND ROBERT SOBOTTA Fit für ehrenamtliche Sprachlernangebote – Qualifizierungsmaßnahmen in der Diskussion ... 55 Workshop 7 ANGELIKA GÜTTL-STRAHLHOFER UND THOMAS UNTERHOLZER Zielgruppengerecht digitale (Lern-)Angebote für Ehrenamtliche erstellen ... 57 Workshop 8 DOROTHEA REEPS, DOROTHEA SPANIEL-WEISE UND JANETTE UHLMANN Zwischen Erfahrungswissen und Empirie – Wissenschaftliche Arbeiten im Kontext der Spracharbeit mit Geflüchteten und Migrant*innen ... 59 Workshop 9 MARLIS SCHEDLER UND VERONIKA SEIDEL Grammatik schon beim Start? Ja, gut dosiert und visualisiert! ... 62 RICO EHREN UND ANN-KATHRIN KOBELT Fazit, Evaluation und Ausblick ... 67 ANNEGRET MIDDEKE Nachwort: „Einheit und Vielfalt?“ ... 71 Dank ... 75 Anhang ANN-KATHRIN KOBELT, FRAUKE AL MOHAMMAD, SOLVEIG BUDER, MICHAEL DOBSTADT, MARCUS OERTEL UND MICHAEL ROLLBERG Handlungsempfehlungen für Deutsch, (Aus)Bildung und Arbeit für Migrant*innen in Dresden ... 77 Graphic Recording ... 85 Übersicht (ausgewählter) Deutschlehrwerke (Workshop 4) ... 86 Zugang zu Landessprachkursen (Workshop 5) ... 90 Spracherwerb und Verständigung (Workshop 5) ... 91 Personenregister ... 92

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Shellhorn, Wendy Lauran Struchen. « Maternal interaction style, reported experiences of care, and pediatric health care utilization ». [Tampa, Fla] : University of South Florida, 2006. http://purl.fcla.edu/usf/dc/et/SFE0001478.

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Strejčková, Lucie. « Business Intelligence ». Master's thesis, Vysoká škola ekonomická v Praze, 2011. http://www.nusl.cz/ntk/nusl-124609.

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The diploma project deals with the issue of Business Intelligence, it focuses on tools used for data analysis and presentation. The project is divided into two parts -- the theoretical and practical one. The theoretical one applies to the characterization of the principle, the description of the architecture components and presentation techniques. The aim of this project is to suggest new suitable analytical tools and graphic interface of BI system for small and medium-sized businesses. Using selected tools is illustrated on a specific example.

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Cattermole, Grant. « School reports : university fiction in the masculine tradition of New Zealand literature ». Thesis, University of Canterbury. English, 2011. http://hdl.handle.net/10092/9709.

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This thesis will investigate the fictional discourse that has developed around academia and how this discourse has manifested itself in the New Zealand literary tradition, primarily in the works of M.K. Joseph, Dan Davin and James K. Baxter. These three writers have been selected because of their status within Kai Jensen's conception of “a literary tradition of excitement about masculinity”; in other words, the masculine tradition in New Zealand literature which provides fictional representations of factual events and tensions. This literary approach is also utilised in the tradition of British university fiction, in which the behaviour of students and faculty are often deliberately exaggerated in order to provide a representation of campus life that captures the essence of the reality without being wholly factual. The fact that these three writers attempt, consciously or unconsciously, to combine the two traditions is a matter of great literary interest: Joseph's A Pound of Saffron (1962) appropriates tropes of the British university novel while extending them to include concerns specific to New Zealand; Davin's Cliffs of Fall (1945), Not Here, Not Now (1970) and Brides of Price (1972) attempt to blend traditions of university fiction with the masculine realist tradition in New Zealand literature, though, as we will see, with limited success; Baxter's station as the maternal grandson of a noted professor allows him to criticise the elitist New Zealand university system in Horse (1985) from a unique position, for he was more sympathetic towards what he considered the working class “peasant wisdom” of his father, Archie, than the “professorial knowledge” of Archie's father-in-law. These three authors have been chosen also because of the way they explore attitudes towards universities amongst mainstream New Zealand society in their writing, for while most novels in the British tradition demonstrate little tension between those within the university walls and those without, in New Zealand fiction the tension is palpable. The motivations for this tension will also be explored in due course, but before we can grapple with how the tradition of British university fiction has impacted New Zealand literature, we must first examine the tradition itself.

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Dlouhý, Radim. « Analýza a optimalizace procesu tvorby manažerského reportu v bankovní instituci ». Master's thesis, Vysoká škola ekonomická v Praze, 2015. http://www.nusl.cz/ntk/nusl-203908.

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This masters thesis tackles the issue of process management in a financial institution, spe-cifically the analysis and optimization of the creation of a certain management report. The first part of this masters thesis is dedicated towards explaining basic theoretical concepts, which will help the reader correctly understand the rest of the paper. There you can find explained the principles of reporting and process management, which will introduce the reader to the issues of processes, their analysis and subsequent optimization. The practical portion first introduces the reader to the particular financial institution, then it describes the organization structure and the activities of data governance, thorough analysis of the particular process, the identification of and a suggestion of optimization which should lead to a more effectively working employees as well as the elimination of narrow places of the process. The conclusion of the masters thesis introduces steps of real implementation of the suggested solutions.

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Marone, David Paul. « The U.S. footprint on the Arabian Peninsula can we avoid a repeat of the pullout from Saudi Arabia ? » Thesis, Monterey, Calif. : Naval Postgraduate School, 2009. http://edocs.nps.edu/npspubs/scholarly/theses/2009/Dec/09Dec%5FMarone.pdf.

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Thesis (M.A. in National Security Affairs)--Naval Postgraduate School, December 2009.
Thesis Advisor(s): Kadhim, Abbas ; Russell, James. "December 2009." Author(s) subject terms: Saudi Arabia, Bahrain, Oman, Oil Rentier, Foreign policy, Wahhabism, domestic policy. Description based on title screen as viewed on Jan. 27, 2010. Includes bibliographical references (p. 59-62). Also available in print.

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Moreno, Gerardo Bullock Lyndal M. « Frequency and quality of the implementation of functional behavioral assessments as reported by educators ». [Denton, Tex.] : University of North Texas, 2008. http://digital.library.unt.edu/permalink/meta-dc-9749.

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Garavello, Silvia <1975&gt. « ANALISI DEI REPERTI FAUNISTICI PROVENIENTI DAI RECENTI SCAVI DI COMACCHIO (FE) ». Master's Degree Thesis, Università Ca' Foscari Venezia, 2012. http://hdl.handle.net/10579/1582.

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Il presente elaborato propone i risultati dello studio del campione faunistico recuperato durante le campagne di scavo di Piazza XX Settembre a Comacchio (FE), da contesti afferenti ad un periodo compreso tra la seconda metà del VI e l’inizio del XVI secolo. Lo studio ha previsto una fase di indagine analitica (determinazione anatomica, della specie, stima dell’età di morte, del sesso, misurazioni osteometriche, studio tafonomico) e quantitativa (conteggio del numero dei resti, del numero minimo degli individui, stima della resa carnea). I dati ottenuti sono stati poi confrontati con i risultati provenienti da siti cronologicamente affini per tentare delle valutazioni di tipo economico-alimentare.

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Kamoyo, Memory. « A review of the use of scientific knowledge in environmental impact assessment reports for dam construction ». Thesis, Uppsala universitet, Institutionen för geovetenskaper, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-421468.

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It is estimated that 3,700 major dams are either planned or under construction, notably in developing countries. Dams can pose a threat to fish diversity, as one of the biggest impacts of damming is habitat fragmentation. Damming causes the isolation of small fish habitats, which reduces genetic diversity and expose fish species to vulnerability of local extinction, hence habitat fragmentation is one of the causes of biological loss. There are tools, which are used to balance between development and biodiversity conservation. One of the tools, which is commonly used, is environmental impact assessment (EIA). EIA uses both science and political knowledge. Science knowledge is important in EIA process as it is required to generate reliable baseline information, impact prediction and effective proposed mitigation. However, no studies have investigated the extent of the use of science knowledge in EIA for hydropower dams in Southern Africa. The purpose of this study is to examine the usage of scientific knowledge in environmental impact statements (EIS), focusing on EISs of dam construction on the impact of habitat fragmentation in fish. Ten EISs were collected from Lesotho, Malawi, Mozambique, Zambia, Zimbabwe. These countries were chosen because they are the hotspots for hydropower planning. I conducted EIS quality review to answer the research purpose. I adopted and used EIS quality review package, which was developed by Lee and Colley (1991), to assess the content and quality of EISs. The results show that the use of science knowledge varies from one EIS to the other, and from one area of review to the other. The baseline and mitigation areas of EISs aligned with the scientific consensus the best, while the identification, prediction and evaluation of impacts area is generally unsatisfactory. Overall, six EISs out of ten EISs use science in a satisfactory way, and four EISs are unsatisfactory, meaning they attempted or completely omitted methods for assessing the ecological concept of fragmentation. I recommend a comprehensive study to review the use biodiversity concepts and methods in EISs for all the dams in southern Africa.

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Quitério, Tânia da Conceição. « O impacto da alteração do regime de reporte de prejuízos fiscais na rendibilidade dos investimentos das empresas ». Master's thesis, FEUC, 2015. http://hdl.handle.net/10316/28513.

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Trabalho de projeto do mestrado em Economia (Economia Industrial), apresentada à Faculdade de Economia da Universidade de Coimbra, sob a orientação de António Manuel Ferreira Martins.
Em 2014, Portugal mudou as suas normas relativamente ao reporte de prejuízos em sede de IRC, permitindo que as empresas pudessem reportar esses prejuízos por mais 7 anos do que era possível anteriormente. No entanto, o impacto sobre os investimentos é ainda incerto, dada a limitação imposta em cada ano ao montante de perda a reportar. Este trabalho pretende estudar o impacto da nova lei sobre um investimento incremental, que gera prejuízos nos seus primeiros anos. Foram usados dados históricos de uma empresa e, os mesmos, projetados para os anos seguintes, de forma a poderem ser analisados os cash-flows do projeto. Por fim, serão calculados indicadores de avaliação dos investimentos para o novo regime, para o regime de dedução anterior e para um regime em que não é permitida a dedução dos prejuízos fiscais. Os resultados obtidos apontam para que a rendibilidade do projeto da empresa aumente com o novo regime de dedução. No entanto, com o aumento do número de períodos é esperada uma diminuição relativa do valor destes benefícios, fazendo com que o aumento da rendibilidade seja menos significativo. E, adicionalmente, o incremento na rendibilidade não é, neste caso, de grande impacto quantitativo.

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Freiberg, Alexander. « Das "Leucine-Rich-Repeat" im Invasionsprotein Internalin B : Stabilität und Faltung eines Solenoidproteins ». [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=974876089.

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Freiberg, Alexander. « Das "Leucine-Rich Repeat" im Invasionsprotein Internalin B : Stabilität und Faltung eines Solenoidproteins ». Phd thesis, Universität Potsdam, 2004. http://opus.kobv.de/ubp/volltexte/2005/253/.

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Für das Verständnis der Strukturbildung bei Proteinen ist es wichtig, allgemein geltende Prinzipien der Stabilität und Faltung zu verstehen. Bisher wurde viel Arbeit in die Erörterung von Gesetzmäßigkeiten zu den Faltungseigenschaften von globulären Proteinen investiert. Die große Proteinklasse der solenoiden Proteine, zu denen z. B. Leucine-Rich Repeat- (LRR-) oder Ankyrin-Proteine gehören, wurde dahingegen noch wenig untersucht. Die Proteine dieser Klasse sind durch einen stapelförmigen Aufbau von sich wiederholenden typischen Sequenzeinheiten gekennzeichnet, was in der Ausbildung einer elongierten Tertiärstruktur resultiert. In der vorliegenden Arbeit sollte versucht werden, die Stabilität und Faltung eines LRR-Proteins mittels verschiedener biophysikalischer Methoden zu charakterisieren. Als Untersuchungsobjekt diente die für die Infektion ausreichende zentrale LRR-Domäne des Invasionsproteins Internalin B (InlB₂₄₁) des Bakteriums Listeria monocytogenes. Des weiteren sollten die Integrität und die Stabilitäts- und Faltungseigenschaften der sogenannten Internalin-Domäne (InlB₃₂₁) untersucht werden. Hierbei handelt es sich um die bei allen Mitgliedern der Internalinfamilie vorkommende Domäne, welche aus einer direkten Fusion des C-terminalen Endes der LRR-Domäne mit einer Immunglobulin (Ig)-ähnlichen Domäne besteht.

Von beiden Konstrukten konnte eine vollständige thermodynamische Charakterisierung, mit Hilfe von chemisch- bzw. thermisch-induzierten Faltungs- und Entfaltungsübergängen durchgeführt werden. Sowohl InlB₂₄₁ als auch InlB₃₂₁ zeigen einen reversiblen und kooperativen Verlauf der chemisch-induzierten Gleichgewichtsübergänge, was die Anwendung eines Zweizustandsmodells zur Beschreibung der Daten erlaubte. Die zusätzliche Ig-ähnliche Domäne im InlB₃₂₁ resultierte im Vergleich zum InlB₂₄₁ in einer Erhöhung der freien Enthalpie der Entfaltung (8.8 kcal/mol im Vergleich zu 4.7 kcal/mol). Diese Stabilitätszunahme äußerte sich sowohl in einer Verschiebung des Übergangsmittelpunktes zu höheren Guanidiniumchlorid-Konzentrationen als auch in einer Erhöhung der Kooperativität des Gleichgewichtsübergangs (9.7 kcal/mol/M im Vergleich zu 7.1 kcal/mol/M). Diese Beobachtungen zeigen dass die einzelnen Sequenzeinheiten der LRR-Domäne nicht unabhängig voneinander falten und dass die Ig-ähnliche Domäne, obwohl sie nicht direkt mit dem Wirtszellrezeptor während der Invasion interagiert, eine kritische Rolle für die in vivo Stabilität des Internalin B spielt. Des weiteren spiegelt die Kooperativität des Übergangs die Integrität der Internalin-Domäne wieder und deutet darauf hin, dass bei beiden Proteinen keine Intermediate vorliegen.

Kinetische Messungen über Tryptophanfluoreszenz und Fern-UV Circulardichroismus deuteten auf die Existenz eines relativ stabilen Intermediates auf dem Faltungsweg der LRR-Domäne hin. Faltungskinetiken aus einem in pH 2 denaturierten Zustand zeigten ein reversibles Verhalten und verliefen über ein Intermediat. Eine Erhöhung der Salzkonzentration des sauer-denaturierten Proteins führte zu einer Kompaktierung der entfalteten Struktur und resultierte im Übergang zu einem alternativ gefalteten Zustand. Bei der Internalin-Domäne deuteten kinetische Messungen des Fluoreszenz- und Fern-UV Circulardichroismus-Signals während der Entfaltung möglicherweise auf die Präsenz von zwei Prozessen hin. Der erste langsame Entfaltungsprozess kurz nach dem Übergangsmittelpunkt zeigte eine starke Abhängigkeit von der Temperatur, während der zweite schnellere Prozess der Entfaltung stärker von der Guanidiniumchlorid-Konzentration abhing. Renaturierungskinetiken zeigten das Auftreten von mindestens einem Faltungsintermediat. Kinetische Daten aus Doppelsprungexperimenten lieferten für die Erklärung der langsamen Faltungsphase zunächst keinen Hinweis auf dass Vorliegen einer Prolinisomerisierungsreaktion. Die vollständige Amplitude während der Renaturierung konnte nicht detektiert werden, weswegen von einer zweiten schnellen Phase im Submillisekundenbereich ausgegangen werden kann.

Die Ergebnisse der Faltungskinetiken zeigen, dass die InlB-Konstrukte als Modelle für die Untersuchung der Faltung von Solenoidproteinen verwendet werden können.

To understand the processes of protein structure formation, it is necessary to investigate protein stability and protein folding kinetics. The focus of many folding studies has been directed at small, globular proteins. The larger class of solenoid proteins, including leucine-rich repeat (LRR) and ankyrin proteins, has not been extensively investigated. These proteins contain tandem repeat motifs, and their tertiary structure consists of a regular linear array of modules that stack to form non-globular elongated or supercoiled structures. In the present work, the folding and stability of the central LRR domain of the invasion protein internalin B (InlB₂₄₁) from the bacterium Listeria monocytogenes was characterized using different biophysical techniques. In addition, the integrity, stability and folding behavior of the so-called internalin-domain (InlB₃₂₁) was investigated. In this single domain, which is found in all members of the internalin-family, an immunoglobulin (Ig)-like domain is directly fused to the C-terminal end of the LRR domain.

A complete thermodynamic characterization of the stability of both constructs was performed, using chemical- and temperature-induced folding and unfolding transitions. The reversible and cooperative equilibrium transition of InlB₂₄₁ and InlB₃₂₁ allowed the use of a two-state model for the description of the data points. The additional Ig-like domain present in InlB₃₂₁ resulted in an increase of the unfolding free energy (8.8 kcal/mol compared to 4.7 kcal/mol). This resulted both, from a shift of the transition midpoint to higher denaturant concentration, and from an increase in the m-value, the denaturant dependence of the unfolding free energy (9.7 kcal/mol/M compared to 7.1 kcal/mol/M). These observations suggest that the unravelling of the individual structural repeats in the LRR region is a cooperative process and that the tight fusion with the Ig-like domain leads to a dramatically increased stability in vivo without interfering with the functionality of the protein. In addition, the cooperativity of the equilibrium transition reflects the integrity of the internalin-domain, and suggests that both InlB fragments unfold without significantly populated equilibrium intermediates.

Kinetic measurements with tryptophan fluorescence and far-UV circular dichroism are indicative for the existence of a relative stable intermediate on the folding pathway of the LRR domain. Refolding kinetics from an acid-denatured state showed a reversible behavior and passes off an intermediate. An increase in the salt concentration of the acid-denatured protein results in a transition of the unfolded structure to a compact and alternatively folded state. Unfolding kinetics of the internalin-domain measured by fluorescence and far-UV circular dichroism are indicative for the possible presence of two processes. The first slow unfolding process after the transition midpoint showed a strong dependence on temperature, whereas the second and faster unfolding process showed a stronger dependence on the denaturant concentration. Renaturation kinetics indicated the existence of at least one folding intermediate. Preliminary double-mixing experiments revealed no evidence for a rate-limiting proline isomerization reaction. It was not possible to detect the complete amplitude of the renaturation reaction, suggesting existence of a second faster phase occuring in the submillisecond range.

The results on folding kinetics prove the InlB constructs to be suitable models for the investigation of solenoid protein folding by techniques of high structural resolution.

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Jara, Pintos Cynthia Mireya. « Influ?ncia do alargamento apical no reparo das periodontites apicais : estudo em ratos ». Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2016. http://tede2.pucrs.br/tede2/handle/tede/6832.

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Introduction: Instrumentation is considered one of the most important steps in endodontic treatment. The literature is controversial with regards to the optimal apical enlargement, especially as to the repair of periapical lesions, proposing different protocols and instrumentation techniques. This study aimed to evaluate the influence of apical enlargement in the process of apical repair in rats. Methods: 24 Wistar rats were divided into 03 experimental groups. Periapical lesion of the first molars on both sides was induced, leaving the root canals exposed to the oral cavity for three weeks. Group 01 consisted of preparation until a #20 file, Group 02 until #25 and Group 03 until #30 file. One molar has been instrumented, while the opposite side was a negative control for apical repair. Root canals were filled 0.5 mm from the apex, with single cone technique of gutta-percha and sealer. After three weeks, the animals were euthanized. Periapical repair was evaluated by digital radiographs and optical microscopy of histological sections (HE) and the data were analyzed using ANOVA test and Kruskal-Wallis test, aiming to establish whether the progressive increase of apical preparation provides an additional benefit in apical repair. Results: Periapical repair was evaluated using digital radiographs. Radiographic limits of the lesions were marked, finding a significant difference (p ? 0.05) in the repair in favor of all groups compared to their respective controls. These results were supported by histological analysis. There was a trend towards improvement in the periapical healing for the larger apical enlargement (group 3). Conclusion: No significant difference was found between the three experimental groups, but it was possible to appreciate radiographically an improving trend for the group of more instrumentation (third group: #30).
Introdu??o: A instrumenta??o ? considerada uma das etapas mais importantes no tratamento endod?ntico. A literatura ? inconclusiva quanto ao alargamento ideal de prepara??o apical, propondo diferentes protocolos e t?cnicas de instrumenta??o, principalmente no que tange ao seu efeito no reparo de les?es periapicais. Portanto, o objetivo deste estudo foi avaliar a influ?ncia do alargamento apical no reparo dos tecidos periapicais de ratos com periodontite apical induzida. M?todos: Foram utilizados 24 ratos Wistar distribu?dos em 03 grupos experimentais. A primeira fase consistiu na indu??o da forma??o das les?es periapicais nos primeiros molares inferiores de ambos os lados, deixando os canais radiculares expostos ao meio oral por tr?s semanas. A seguir, no Grupo 01 o preparo pela t?cnica seriada foi realizado at? o instrumento #20, no grupo 02 at? #25 e no Grupo 03 at? #30. Os canais foram obturados 0,5 mm aqu?m do forame apical, pela t?cnica do cone ?nico de guta-percha e cimento AH Plus e posteriormente selados com cimento de ion?mero vidro. Ap?s tr?s semanas foi realizada a eutan?sia. O reparo periapical foi avaliado atrav?s de radiografias digitais e analises histol?gica e os dados obtidos foram analisados por meio dos testes de ANOVA e Kruskal-Wallis com o objetivo de determinar se o aumento progressivo da prepara??o apical fornece um benef?cio adicional na repara??o apical. Resultados: Foi observada uma diferen?a significativa (p?0,05) no reparo a favor de todos os grupos experimentais quando comparando aos seus respectivos controles. Estes resultados foram respaldados pela analise histol?gica. Radiograficamente, observou-se leve tend?ncia de melhora na cicatriza??o periapical para o maior alargamento apical (grupo 3). Conclus?o: Nenhuma diferen?a significativa foi encontrada entre os tr?s grupos experimentais, mas foi poss?vel apreciar radiograficamente uma tend?ncia de melhoria no grupo de maior instrumenta??o (grupo 3: # 30).

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Carretto, Elena. « Tumori rari pediatrici come spia di sindromi genetiche : un report dal progetto TREP (Tumori Rari in Età pediatrici) su carcinoma renale e feocromocitoma ». Doctoral thesis, Università degli studi di Padova, 2009. http://hdl.handle.net/11577/3426505.

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We consider rare tumors in pediatric age those tumors with an incidence less than 2 cases/1.000.000 children/year, which are not included in treatment protocols of the Associazione Italiana di Emato-Oncologia Pediatrica (AIEOP). These tumors are object of a national project called TREP, aiming to register patients with rare tumors and promote studies of these cancers. We hypothesize that infancy-onset of typically adulthood tumors, extremely rare in pediatric age, could be the expression of an underlying predisposing genetic condition. In fact, the earliest is the age of onset, the least is the exposition to environmental factors. The renal cell carcinoma (RCC) occurs in a sporadic form or as part of genetic diseases, such as Von Hippel-Lindau disease. Likewise, phechromocytoma and paraganglioma occur in a sporadic form or associated with hereditary disorders such as Von Hippel-Lindau disease, Hereditary Paraganglioma-Pheochromocytoma Syndrome, Multiple endocrine neoplasia type 2 (MEN2) syndrome and neurofibromatosis, type 1. AIM We report the preliminary results of a retrospective and prospective study of a TREP project aiming to evaluate the prevalence of main hereditary syndromes associated with renal cell carcinoma and pheochromocytoma in pediatric age. MATERIALS AND METHODS. Patients diagnosed with renal cell carcinoma or pheochromocitoma from January 2000 until May 2009 (age at diagnosis <18 years) and registered in the TREP, alive, with or without disease, were considered for this study. These patients were registered at 18 reference Italian Pediatric Surgery or Oncology centers. Patients were contacted by the Reference Center in order to obtain blood DNA to detect germ-line mutation of the following genes: VHL (Von Hippel-Lindau disease): in renal cell carcinomas and pheochromocytomas RET (MEN2), SDHB, SDHC, SDHD (Hereditary Paraganglioma-Pheochromocytoma Syndrome): in pheochromocytomas only. For patients with renal cell carcinoma, referring clinicians were asked to complete a questionnaire, aimed to enlighten the presence of signs and symptoms suggestive of the possible associated syndromes. Mutation scanning of the VHL gene for identification of point or small size mutations was conducted on the entire coding sequence and intron-exon boundaries, by PCR amplification, DHPLC and direct sequence analysis. Quantitative Real Time PCR for the identification of deletions of part or the entire gene, was performed on genomic DNA fragments representing each VHL exon. Genetic analysis of RET, SDHB, SDHC, SDHD genes was conducted by direct sequencing of coding sequence and intron-exon boundaries. For RET gene, exons 8, 10, 11, 13, 15 and 16 were sequenced. For SDHx genes, all 8 SDHB exons, all 6 SDHC and 4 SDHD exons were entirely sequenced. Rearrangement analysis was conducted by MLPA (Multiple Ligation Probe Assay). Finally we analyzed clinical data in relation to the presence of genetic syndromes. RESULTS Genetic analysis was performed in 13 on 32 eligible patients with renal cell carcinoma. In all cases no mutation were found. In no case the questionnaire, completed in 11 patients, suggested the presence of an underlying genetic syndrome. 14 patients with pheochromocytoma (on 20 eligible) underwent the genetic analysis: we found a germ-line mutation in 7 (2 VHL, 1 SDHD, 4 SDHB) with an overall prevalence of 50%. When possible, genetic analysis was extended to family members: until now we found 9 mutations in SDHB gene and 1 mutation of SDHD. From a clinical point of view our data support the role of a complete surgery of the tumor. Patients with genetic syndrome did not significantly differ from nonsyndromic ones in terms of clinical manifestations and prognosis. CONCLUSIONS As far as renal cell carcinoma is concerned, the limited number of cases analyzed until today does not allow to draw any significant conclusion. Particularly, we could not detect an association with VHL disease. We found an high prevalence (50%) of genetic disorders associated with pheochromocytoma otherwise labeled "sporadics". This prevalence is higher than what reported in adulthood, confirming that the incidence of pheochromocytoma in pediatric age is more frequently associated to an inherited disorder. Our data show that some rare tumors in pediatric age can be the first manifestation of a genetic syndrome. Both renal cell carcinoma and pheochromocytoma clinical outcome strictly depend on completeness of surgery; however these patients need to be considered also from a genetic point of view for the possible implication that a family cancer syndrome can have for the patient and other family members.
Consideriamo "tumori rari in età pediatrica" quei tumori con un incidenza inferiore ai 2 casi/1.000.000 bambini/anno e che non sono inclusi in protocolli di trattamento dell'Associazione Italiana di Emato-Oncologia Pediatrica (AIEOP). Questi Tumori sono l'oggetto di un progetto nazionale denominato Progetto TREP che si propone fra gli obiettivi la registrazione dei pazienti con tumore raro e l'avvio di studi a loro dedicati. Questo studio si basa sull'ipotesi che l'insorgenza in un bambino di un tumore tipico dell'età adulta, ma estremamente raro in età pediatrica possa essere l'espressione di una condizione genetica predisponente. Infatti più è precoce l'età di insorgenza di un tumore meno è facile che sia avvenuta un' esposizione a fattori ambientali. Il carcinoma renale (CR) può presentarsi in forma sporadica o come parte del quadro clinico di alcune malattie genetiche, tra cui la malattia di Von Hippel-Lindau. Analogamente feocromocitoma e paraganglioma possono presentarsi sia in forma sporadica che associati ad alcune malattie geneticamente trasmissibili tra cui la malattia di Von Hippel-Lindau, la sindrome del feocromocitoma/paraganglioma ereditario, la sindrome da neoplasie endocrine multiple tipo 2 (MEN 2) e la neurofibromatosi tipo 1. SCOPO Nella tesi riportiamo i dati preliminari di uno studio retrospettivo e prospettico condotto nell'ambito del Progetto TREP, che ha lo scopo di valutare la prevalenza delle principali sindromi genetiche associate a carcinoma renale e a feocromocitoma in età pediatrica. MATERIALI E METODI Sono stati considerati i pazienti con carcinoma renale e con feocromocitoma registrati nel Progetto TREP di età <18 anni, con diagnosi da gennaio 2000 a maggio 2009 e vivi, con o senza evidenza di malattia, al momento dello svolgimento di questo studio. Questi pazienti sono stati registrati da 18 Centri italiani di Chirurgia o Oncologia Pediatrica. I pazienti, sono stati ricontattati dal medico del centro di riferimento e gli è stato proposto di essere sottoposti a prelievo di sangue periferico per ricerca di alterazioni genetiche. In particolare sono state ricercate: VHL (malattia di Von Hippel-Lindau): sia per i carcinomi renali che per i feocromocitomi RET (MEN2), SDHB, SDHC e SDHD (sindrome del feocromocitoma/paraganglioma familiare): solo per i feocromocitomi. Inoltre per i carcinomi renali è stato compilato dal clinico che aveva in cura il paziente un questionario che cercava di identificare sintomi e segni delle possibili sindromi associate. L'analisi delle mutazioni del gene VHL per l'identificazione di mutazioni puntiformi o di piccola taglia è stata condotta sull'intera sequenza codificante e sulle zone di giunzione introne-esone attraverso amplificazione in PCR, DHPLC e sequenziamento diretto. La PCR Real Time quantitativa è stata utilizzata su frammenti di DNA genomico rappresentanti ogni esone del gene VHL per identificare delezioni di parti o dell'intero gene. L'analisi genetica del gene RET e dei geni SDHB, SDHC ed SDHD è stata condotta mediante sequenziamento diretto delle regioni codificanti e delle regioni introniche fiancheggianti. Per il gene RET sono stati sequenziali gli esoni 8, 10, 11, 13, 15 e 16. Per quanto riguarda i geni SDHx sono stati sequenziati interamente gli 8 esoni di SDHB, i 6 di SDHC e i 4 di SDHD; inoltre è stata condotta l'analisi dei riarrangiamenti mediante MLPA (Multiple Ligation Probe Assay). Abbiamo infine analizzato i risultati del trattamento correlandoli alla presenza di una sindrome genetica. RISULTATI Per quanto riguarda i carcinomi renali sono stati identificati 32 pazienti eligibili al nostro studio. La scheda anamnestica è stata somministrata in 11 pazienti, e non ha posto in nessun paziente il sospetto clinico di una sindrome genetica associata. L'analisi genetica per VHL è stata condotta in 13 pazienti ed in tutti i casi ha dato esito negativo. Clinicamente la prognosi è apparsa correlata con lo stadio e quindi con la chirurgia: i pazienti con tumore localizzato asportato completamente hanno mostrato un' ottima sopravvivenza. Per i feocromocitomi l'analisi genetica è stata condotta in 14 pazienti su 20 eligibili ed ha evidenziato una mutazione germinale in 7 (2 VHL, 1 SDHD, 4 SDHB) con una prevalenza totale del 50%. Ove possibile l'analisi genetica è stata estesa anche ai familiari: finora sono stati identificati 9 familiari con mutazione del gene SDHB e uno con mutazione di SDHD. Dal punto di vista clinico i nostri dati confermano l'importanza di una chirurgia completa del tumore. Non è stata riscontrata una differenza significativa nelle manifestazioni cliniche e nella prognosi dei pazienti sindormici quando confrontati a quelli non sindormici. CONCLUSIONI Per quanto riguarda il carcinoma renale, non siamo riusciti ad evidenziare una associazione con la sindrome di VHL, ma la limitata numerositÃ del campione esaminato ci permette di trarre solo delle conclusioni preliminari. Nei feocromocitomi abbiamo riscontrato un' elevata prevalenza (50%) di sindromi genetiche associate. Tale frequenza è superiore rispetto a quanto riportato in casistiche di pazienti adulti confermando che l'insorgenza di un feocromocitoma in età pediatrica è maggiormente associata ad una sindrome genetica. I nostri dati dimostrano che alcuni tumori rari per l'età pediatrica possono rappresentare la prima manifestazione di una sindrome genetica. Il risultato del trattamento è strettamente correlato all' esito della chirurgia sia per i carcinomi renali che per il feocromocitoma. E' però importante sottolineare che oltre le problematiche inerenti al trattamento vanno considerate quelle di tipo genetico per le implicazioni che una family cancer syndrome può avere per la famiglia e il paziente stesso.

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Araújo, Nadja Maria Souza. « Viver mais e melhor : o programa Globo Reporte e as Redes de Sentido da Saúde ». reponame:Repositório Institucional da FIOCRUZ, 2011. https://www.arca.fiocruz.br/handle/icict/5749.

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Brasil. Ministério da Saúde. Gabinete do Ministro. Brasília, DF, Brasil.
Esta dissertação objetiva entender a relação entre os sentidos da promoção da saúde propostos pelo Sistema Único de Saúde e os sentidos propostos pela mídia e de como se dá a mediação entre esses últimos e a população. O principal eixo teórico que permitiu cumprir os objetivos foi o da produção social dos discursos, particularmente a abordagem de uma teoria dos discursos sociais. A partir de um exame das concepções de Promoção da Saúde, foram analisadas algumas das edições do Programa Globo Repórter com temas da Saúde e elaborados Mapas do Mercado Simbólico (fontes e fluxos da comunicação recebida) e Mapas das Redes de Compartilhamento dos sentidos com grupos de pessoas nas cidades do Rio de Janeiro - RJ e na cidade de Aracaju - SE. A pesquisa apontou que os sentidos da promoção da saúde articulados pela mídia apresentam uma abordagem comportamental e individualista e deixam de levar e conta as condições de vida dos indivíduos e coletivos. A construção dos mapas mostrou, porém, que apesar da centralidade da mídia, outras instituições constroem também sentido de saúde e concorrem com esta por uma melhor posição discursiva, dentre elas as instituições públicas de saúde. A audiência, por sua vez, se apodera desse discurso a partir de seu próprio repertório, de acordo com os contextos e articula e compartilha estes sentidos em seus ambientes de convivência, compondo uma rede de semiose com possibilidades infinitas de desdobramentos.
This dissertation aims to understand the relationship between the senses of health promotion proposed by the Brazilian public health system (SUS) and those proposed by mass media and also the mediation processes between the latter and the audience. The main theoretical axis that allowed us to achieve our goal was the social production of discourses, specially the approach to a theory of social discourses. From an examination of the concepts of Health Promotion, some editions of a TV news program, Globo Repórter - which featured stories about health issues - were analyzed, and Symbolic Market Goods (sources and flows of incoming messages) and Senses Sharing Networks maps were designed by groups of residents of the cities of Rio de Janeiro - RJ and Aracaju – SE. The survey showed that the senses of health promotion presented by the mass media have a behavioral and individualistic approach and fail to take into account the life conditions of individuals and communities. However, the construction of the maps showed that, despite the centrality of the mass media, other institutions also build senses of health and compete with it for a better discursive position, among them public health institutions. The audience, in turn, takes hold of this discourse from its own repertoire, according to the contexts, and articulates and shares these senses in their own environments, creating a network of semiosis with limitless possibilities unfolding.

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Greif, Tomáš. « Vizualizace dat v podnikové praxi ». Master's thesis, Vysoká škola ekonomická v Praze, 2011. http://www.nusl.cz/ntk/nusl-71839.

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Diploma thesis describes basics terms of data visualization elements in the theoretical part. There are the following main topics covered -- importance of data visualization in business management, description and application of main chart types, design and usage of complex visualizations (dashboards), key features of modern data visualization technology. Theoretical grounds are then applied on real examples from different companies in order to show how managers can benefit from good data visualization techniques. Examples from significantly different areas were chosen -- financial management, operations management, analysis of process efficiency.

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Brož, Ondřej. « Vizualizace dat v reportingových nástrojích BI ». Master's thesis, Vysoká škola ekonomická v Praze, 2011. http://www.nusl.cz/ntk/nusl-72549.

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This diploma thesis deals with the Business Intelligence reporting tools and more especially with their data visualization capabilities. There are a lot of different reporting and analytic tools focusing for example on ad-hoc query, tool for creating dashboards or scorecards, visual discovery tools and advanced analytical tools for data mining etc. This work, however, is focused especially on selected enterprise reporting tools and data visualization tools as well as their possibilities of data isualizations, i.e. using different types of graphs and other possible advanced data visualizations. The Business Intelligence fundamentals together with the possible the reporting and analysis visual outputs are described at the beginning of this diploma work. The reporting itself is also putted into the context of the Business Intelligence solution. The next section is focus on the visual possibilities of data visualization in BI reporting tools. It means that I created the list of possible BI data visualizations with the detail specifications and showing examples on each of them. After this part, there are shown proper data visualizations for each of the selected BI domains, like finance, sales, marketing and human resources. This output belong to one of the main contributions of this work and also could be practically useful in the process of report creation and selection for Clever Decision's BI reporting needs. In the next chapter I compare the visual possibilities for each of the selected BI reporting tools according to Gartner. To this purpose the official documentations will be used as well as my personal experience gained from using some of selected reporting tools. In the last section there are detail views for each of two selected advanced data visualizations including a detail specification on each of them as well as his possible usage in practice and possibilities for further extension as well. The source of information in this section will be professional electronic resources focused on data visualization, design and so on.

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Volf, Roman. « Signální monitoring dodavatelů / zákazníků ». Master's thesis, Vysoká škola ekonomická v Praze, 2012. http://www.nusl.cz/ntk/nusl-165150.

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This thesis deals with the possibilities of using methods of Competitive Intelligence in practice. The main objective of thesis is to design and realize system for collecting, analysis and distribution of data to end users at Crystal Glamour, Ins. To achieve this goal was set several sub - objectives. Analyze the company activities, find its strengths and weaknesses, identify opportunities and threats of company. Based on these data are identified goals of CI and information sources that cover the domain of interest. Then, using software applications from Tovek, spol. Ltd., the relevant articles are searched and analyzed, which are then distributed by the well-arranged report to individual users.

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Volf, Roman. « Competitive Intelligence ve vybrané společnosti ». Master's thesis, Vysoká škola ekonomická v Praze, 2012. http://www.nusl.cz/ntk/nusl-203712.

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This thesis deals with the possibilities of using methods of Competitive Intelligence in practice. The main objective of thesis is to design and realize system for collecting, analysis and distribution of data to end users at Crystal Glamour, Ins. To achieve this goal was set several sub - objectives. Analyze the company activities, find its strengths and weaknesses, identify opportunities and threats of company. Based on these data are identified goals of CI and information sources that cover the domain of interest. Then, using software applications from Tovek, spol. Ltd., the relevant articles are searched and analyzed, which are then distributed by the well-arranged report to individual users.

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Král, Jakub. « Zvýšení informační hodnoty dat pomocí moderních přístupů k vizualizaci ». Master's thesis, Vysoká škola ekonomická v Praze, 2015. http://www.nusl.cz/ntk/nusl-204081.

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This thesis solves the area of data visualization in Business Intelligence. We live in a time when the amount of data grows turbulently. These data need to be processed into a form with added value for management and business objectives of the company. The first task is to adjust the data into the required format, the second task is to choose the appropriate visualization so that the data are providing the highest value. This work deals with data visualization and its main aim is to introduce a different approach to data visualization, which has a higher added value than conventional visualization methods used in dashboards and reports. First of all, theoretical part is describing data area (including the trend of Big Data) and reporting (chapter 3). Followed by chapter 4, which is enriching the reader with the information about the need to deal with data visualization and also with the design theory of visualization components. The theoretical part is finished with fifth chapter. It deals with current trends in the field of data visualization and Business Intelligence and also introduces D3.js library as a tool for creating interactive and efficient visualization components. The practical part is offering suggestions for interactive and dynamic visualization of input data (chapter 6). It presents a convenient way to choose a visualization component with greater value than the traditional static component in the form of a graph or table. Proposals contained in D3.js library are presented to the reader. Chosen components according to the author are interesting from the view of design and, if properly used, offer an innovative approach to data visualization. For purposes of practical examples of visualization, author is using D3.js library and open source tool for Business Intelligence from Pentaho. Practical example is part of the final chapter 7.

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Carvalho, Luiz Carlos Augusto de. « O segmento importa ? Estudo comparativo do atendimento aos padrões de relato da global report initiative pelos relatórios de desempenho socioambiental das concessionárias de energia elétrica brasileiras ». reponame:Repositório Institucional da UFPR, 2016. http://hdl.handle.net/1884/44489.

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Orientador : Prof. Dr. Luiz Panhoca
Dissertação (mestrado) - Universidade Federal do Paraná, Setor de Ciências Sociais Aplicadas, Programa de Pós-Graduação em Contabilidade. Defesa: Curitiba, 2016
Inclui referências : f. 113-124
Área de concentração : Contabilidade e finanças
Resumo: O crescimento da importância de questões socioambientais aumentou a necessidade de as organizações comunicarem aos seus stakeholders, de forma consistente e confiável, suas ações relacionadas ao comprometimento destas com aquelas questões. Quando relacionadas às questões socioambientais, essas necessidades podem ser supridas pelas informações da contabilidade por meio de Relatórios de Desempenho Socioambiental (RDS). No entanto, existem algumas dificuldades relacionadas à elaboração desses relatórios, podendo-se destacar a não obrigatoriedade de apresentação e a falta de padronização. Uma tentativa para o equacionamento dessas dificuldades tem sido proposta pela Global Report Initiative (GRI), por meio do seu modelo padronizado de relatório de sustentabilidade, o qual é o mais aceito atualmente. Entretanto, pesquisas anteriores identificaram que as organizações que adotam o relatório proposto pela entidade não o cumprem na íntegra. Dessa forma, pode-se concluir que a Teoria da Contabilidade não está sendo atendida quanto ao seu Enfoque Ético, o qual preconiza que as informações produzidas devem ser fidedignas e isentas de vieses. Com base nisso, e considerando que no Setor Elétrico Brasileiro (SEB) existe a obrigatoriedade de apresentação de um relatório padronizado baseado nos padrões da Global Report Initiative, a presente investigação teve o propósito de descrever como as concessionárias do setor elétrico em seus RDS, referentes ao ano de 2014, atenderam aos padrões da GRI. A análise de dados consistiu da coleta e verificação de 80 relatórios publicados em 2014 pelas concessionárias. Utilizou-se a técnica de análise do conteúdo de 33 indicadores referentes aos Conteúdos Padrão Gerais propostos pela GRI, tendo sido aplicado o teste Kruskal-Wallis para identificar diferenças entre os segmentos. Foi verificado que o segmento de geração (84,5%) foi o que mais atendeu às Diretrizes GRI, seguido pela distribuição (74,7%) e transmissão (63,9%). Adicionalmente, o teste Kruskal-Wallis revelou que as diferenças encontradas entre os índices são significantes sob o ponto de vista estatístico. Constatou-se que o fator segmento da indústria pode influenciar a qualidade da divulgação socioambiental. Também foi verificado que a ANEEL, a agência que regula o setor, falha em fiscalizar as concessionárias e que a Teoria da Contabilidade não está sendo atendida quanto ao seu Enfoque Ético devido às deficiências encontradas quanto ao conteúdo dos relatórios. Palavras-chave: Setor elétrico. Sustentabilidade. Gestão Ambiental. GRI.
Abstract: The growing importance of environmental issues has also increased the need for organizations to communicate consistently and reliably their actions to stakeholders, given that these somehow contribute to the success of these. When related to environmental issues, these needs can be met by accounting information through sustainability report. However, there are some difficulties related to the preparation of reports, being able to highlight not obligatory presentation and lack of standardization. An attempt for solving these problems has been proposed by the Global Reporting Initiative (GRI), and its report template the most currently accepted. However, previous research has found that companies that adopt the report proposed by the entity did not comply in full. Thus, it can be concluded that the Accounting Theory is not being met as to its Ethical Approach, which holds that the information produced should be reliable and free from bias. In this basis, and considering that the Brazilian Electric Sector there is a requirement to present this document, the present study aimed to describe how the power sector utilities in their report, for the year 2014 met the standards GRI. Data analysis is the collection and analysis of 80 reports published in 2014 by the companies. It was used the technique of analyzing the content of 33 indicators for the proposed by the GRI and applied the Kruskal-Wallis test to identify differences between segments. It was found that the generators (84.5%) was the most met the guidelines GRI, followed by distribution (74.7%) and transmission (63.9%). In addition, the Kruskal-Wallis test showed that the differences between the indices are significant from a statistical point of view. It was found that the industry segment factor can influence the quality of environmental disclosure. It was also found that ANEEL failure to supervise utilities and the Accounting Theory is not being met as to its Ethical Approach due to deficiencies in the contents of the reports. Keywords: Electric Sector. Sustainability. Environmental management. GRI.

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Melo, Julliane Tamara Ara?jo de. « Papel da prote?na de reparo XPC na regula??o das prote?nas de reparo APE1, OGG1 e PARP-1 em c?lulas humanas e de camundongos ». Universidade Federal do Rio Grande do Norte, 2014. http://repositorio.ufrn.br:8080/jspui/handle/123456789/12573.

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Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico
studies using UV as a source of DNA damage. However, even though unrepaired UV-induced DNA damages are related to mutagenesis, cell death and tumorigenesis, they do not explain phenotypes such as neurodegeneration and internal tumors observed in patients with syndromes like Xeroderma Pigmentosum (XP) and Cockayne Syndrome (CS) that are associated with NER deficiency. Recent evidences point to a role of NER in the repair of 8-oxodG, a typical substrate of Base Excision Repair (BER). Since deficiencies in BER result in genomic instability, neurodegenerative diseases and cancer, it was investigated in this research the impact of XPC deficiency on BER functions in human cells. It was analyzed both the expression and the cellular localization of APE1, OGG1 e PARP-1, the mainly BER enzymes, in different NER-deficient human fibroblasts. The endogenous levels of these enzymes are reduced in XPC deficient cells. Surprisingly, XP-C fibroblasts were more resistant to oxidative agents than the other NER deficient fibroblasts, despite presenting the highest of 8-oxodG. Furthermore, subtle changes in the nuclear and mitochondrial localization of APE1 were detected in XP-C fibroblasts. To confirm the impact of XPC deficiency in the regulation of APE1 and OGG1 expression and activity, we constructed a XPC-complemented cell line. Although the XPC complementation was only partial, we found that XPC-complemented cells presented increased levels of OGG1 than XPC-deficient cells. The extracts from XPC-complemented cells also presented an elevated OGG1 enzimatic activity. However, it was not observed changes in APE1 expression and activity in the XPCcomplemented cells. In addition, we found that full-length APE1 (37 kDa) and OGG1- ? are in the mitochondria of XPC-deficient fibroblasts and XPC-complemented fibroblasts before and after induction of oxidative stress. On the other hand, the expression of APE1 and PARP-1 are not altered in brain and liver of XPC knockout mice. However, XPC deficiency changed the APE1 localization in hypoccampus and hypothalamus. We also observed a physical interaction between XPC and APE1 proteins in human cells. In conclusion, the data suggest that XPC protein has a role in the regulation of OGG1 expression and activity in human cells and is involved mainly in the regulation of APE1 localization in mice. Aditionally, the response of NER deficient cells under oxidative stress may not be only associated to the NER deficiency per se, but it may include the new functions of NER enzymes in regulation of expression and cell localization of BER proteins
A maior parte do nosso conhecimento sobre a via de Reparo de Excis?o Nucleot?deos (NER) vem de estudos usando a luz ultravioleta (UV) como fonte de danos no DNA. Contudo, embora os danos no DNA causados pela luz UV sejam relacionados ? ocorr?ncia de mutag?nese, morte celular e tumorig?nese, eles n?o justificam fen?tipos como neurodegenera??o e tumorig?nese observados em pacientes com s?ndromes como Xeroderma Pigmentosum (XP) e S?ndrome de Cockayne (CS), as quais s?o associadas ? defici?ncia na via NER. Adicionalmente, evid?ncias mais recentes indicam o envolvimento da via NER no reparo de 8-oxodG, um substrato t?pico da via de Reparo por Excis?o de Bases (BER). Uma vez que a defici?ncia na via BER resulta em instabilidade gen?mica, doen?as neurodegenerativas e c?ncer, foi investigado neste trabalho o impacto da defici?ncia em XPC nas fun??es da via BER em c?lulas humanas. Foram realizadas an?lises da express?o e da localiza??o celular de APE1, OGG1 e PARP-1, principais enzimas da via BER, em fibroblastos humanos deficientes na via NER. Os resultados demonstraram que os n?veis end?genos de APE1, PARP-1 e OGG1 s?o reduzidos nos fibroblastos deficientes em XPC, os quais foram mais resistentes a diferentes tipos de agentes oxidantes e apresentaram n?veis elevados de 8-oxodG quando comparados aos demais fibroblastos deficientes na via NER. Adicionalmente, altera??es sutis na localiza??o nuclear e mitocondrial de APE1 foram observadas nos fibroblastos deficientes em XPC. Para confirmar o impacto da defici?ncia de XPC na regula??o da express?o e atividade de APE1 e OGG1, foi constru?da uma linhagem complementada com XPC. Embora a complementa??o tenha sido parcial, foi poss?vel observar que os fibroblastos parcialmente complementados com XPC apresentaram n?veis maiores de express?o de OGG1 quando comparados aos fibroblastos deficientes em XPC. Os extratos dos fibroblastos parcialmente complementados com XPC tamb?m apresentaram uma elevada atividade enzim?tica de OGG1. Contudo, n?o foram observadas mudan?as na express?o e atividade de APE1 nos fibroblastos parcialmente complementados com XPC. Adicionalmente, foi poss?vel verificar a presen?a da forma completa de APE1 (37 kDa) e de OGG1-? na mitoc?ndria dos fibroblastos deficientes em XPC e parcialmente complementados com XPC. Por outro lado, observou-se que a express?o de APE1 e PARP-1 n?o ? alterada no c?rebro e f?gado de camundongos knockouts para XPC. Contudo, a defici?ncia em XPC resultou em mudan?as na localiza??o celular de APE1 no hipocampo e hipot?lamo. Ainda, foi observada a ocorr?ncia de uma intera??o f?sica entre as prote?nas XPC e APE1 em c?lulas humanas. Em conclus?o, os dados sugerem que a prote?na XPC possui um papel na regula??o da express?o e da atividade de OGG1 em c?lulas humanas e est? envolvida na regula??o da localiza??o celular de APE1 principalmente em camundongos. Adicionalmente, as respostas celulares dos fibroblastos deficientes na via NER ao estresse oxidativo podem n?o estar associadas ? defici?ncia na via NER per se, mas podem incluir novas fun??es das enzimas da via NER na regula??o da express?o e localiza??o celular das prote?nas da via BER
2020-01-01

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Sathler, Conrado Neves. « Escrita disciplinar e psicologia : laudos como estrategia de controle das populações ». [s.n.], 2008. http://repositorio.unicamp.br/jspui/handle/REPOSIP/269481.

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Orientador: Maria Jose Rodrigues Faria Coracini
Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Estudos da Linguagem
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Resumo: Este trabalho apresenta uma análise de Laudos Psicológicos como textos construídos num imbricamento de discursos científicos, técnicos e administrativos e que são, hoje, na sociedade brasileira, motivo de tensões dentro da categoria profissional dos psicólogos e, também, nas instituições onde são exigidos, requeridos, discutidos, ensinados, regulamentados, rejeitados, refutados e transformados em elementos de disputas jurídicas para reconhecer ou não sua validade. Filiada às teorias da subjetividade, esta tese parte do pressuposto de que o sujeito é um efeito do discurso, construído, portanto, pela linguagem; logo, essa escrita constrói um sujeito em linguagem psicológica; no entanto, o sujeito hegemônico no Laudo Psicológico é o sujeito disciplinar - posto em circulação em linguagem psi -, segundo a lógica dicotômica moderna e, conseqüentemente, sujeito adequado ou inadequado, normal ou anormal, produtivo ou improdutivo ou, em última análise, sujeito disciplinado ou indisciplinado, apropriado ou não, segundo critérios desse dispositivo de poder. Por se tratar de um dos agentes do Biopoder, o Laudo Psicológico se insere nas estratégias de controle da população e nos arquivos do Estado. Porém, estando na ordem do discurso, o próprio Laudo é objeto de controle e o Manual de Elaboração de Documentos Escritos por Psicólogos - corpus da presente pesquisa - se inscreve como instrumento de controle discursivo, enquanto modelo de escrita, e disciplinar, enquanto norma regimental. Ao afirmar que o sujeito é construído em linguagem psi, pode-se ter a impressão de que esse sujeito é definido segundo uma linguagem técnica ou profissional; contudo, na análise de 5 laudos psicológicos, observa-se uma panacéia em discurso já disseminado e naturalizado. As conseqüências éticas e sociais da disseminação do discurso psi vão da banalização dos laudos até o enfraquecimento dos processos jurídicos, educacionais e administrativos neles baseados.
Abstract: This project presents an analysis of Psychological Findings such as texts built in imbricated scientific, technical and administrative discourses, and that today, in the Brazilian society, are reason of tension within the psychologists professional category and that also in the institutions where they are demanded, required, discussed, taught, regulated, rejected, refuted and transformed in elements of juridical disputes to recognize or not its validity. Affiliated to the subjectivity's theories, this thesis understands that the subject is an effect of the constructed discourse, therefore, by language; ergo, this writing builds a subject in psychological language; however, the hegemonic subject in the Psychological Finding is the disciplinary subject - put in psi language, according to the modern dichotomic logic, and consequently, adequate or inadequate subject, disciplined or undisciplined subject, appropriated or not, in accordance to the criteria of this power device. For being one of the Biopower agents, the Psychological Finding is inserted in the population control strategies and in the State archives. However, being in the discourse order, the Finding itself is object of control and the Documents Written by Psychologists Elaboration Manual - corpus of the present research - can be considered an instrument of discursive control, as a writing model, and disciplinary, as a regimental model. When affirming that the subject is built in psi language one can have the impression that this subject is defined according to a technical or professional language; nevertheless, in the analysis of the five psychological findings it is observed a panacea in a already disseminated and naturalized discourse. The ethical and social consequences of the psi discourse dissemination go from the banalization of the findings to the enfeeblement of the juridical, educational and administrative processes based on them.
Doutorado
Lingua Materna
Doutor em Linguística Aplicada

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Biguetti, Claudia Cristina. « Papel das células CCR2+ no processo de reparo ósseo alveolar em camundongos : caracterização histomorfométrica e molecular ». Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/25/25149/tde-17072014-085242/.

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O processo de reparo ósseo depende de uma resposta inflamatória inicial e transitória, a qual envolve a participação de diversos leucócitos, como células da linhagem monócito/macrófago. O receptor CCR2 é importante para o recrutamento de macrófagos durante as respostas imunes, além de ter um papel na regulação da osteoclastogênese. Assim, o objetivo do presente estudo foi investigar papel de células CCR2+ no processo de reparo ósseo alveolar pós-exodontia em camundongos, por meio de análises microscópicas (MicroCT, histomorfometria, análise de birrefringência e imuno-histoquímica) e moleculares (PCRArray) comparativas entre as linhagens C57Bl/6 (WT) e CCR2KO, ao longo dos períodos de 0 hora, 7, 14 e 21 dias pós-exodontia do incisivo superior direito. Como resultado geral das análises microscópicas, constatamos que a ausência de células CCR2+ não afetou o resultado final do reparo ósseo alveolar em camundongos CCR2KO, mas levou a alterações transitórias e estatisticamente significantes (p<0,05) para quantificação de infiltrado inflamatório, vasos sanguíneos, fibroblastos, fibras colágenas, osteoblastos e osteoclastos. Além disso, a ausência de células CCR2+ resultou em diminuição (p<0,05) de células F4/80+ e CCR5+ no infiltrado inflamatório ao longo do processo de reparo ósseo alveolar de camundongos CCR2KO, demonstrando o papel do receptor CCR2 no recrutamento de macrófagos (células F4/80+), bem como sugerindo que as células F4/80+ apresentam dupla positividade para os receptores CCR2 e CCR5. Neste contexto, o receptor CCR5 seria o responsável pela migração remanescente, ainda que reduzida, de células F4/80+ nos animais CCR2KO. Considerando os resultados moleculares, a ausência de CCR2 resultou na alteração da expressão de diferentes marcadores em camundongos CCR2KO, tais como: o fator de crescimento TGF1, marcadores de matriz COL1, MMP1a, MMP2 e MMP9, marcadores ósseos RUNX2, DMP1, RANKL, RANK e CTSK, e marcadores de MSCs CD106, COT-4, NANOG, CD146 e CD105, bem como de marcadores imunológicos como as citocinas IL-6 e TNF-a, receptores de quimiocinas CCR1, CCR5 e CXCR1,e as quimiocinas CCL12, CCL20, CCL25 e CXCL12. Em conclusão, estes resultados indicam que células CCR2+ desempenham diferentes funções no reparo ósseo alveolar em camundongos, influenciando tanto a resposta inflamatória, como os eventos teciduais observados ao longo deste processo.
The bone repair process depends of an initial and transitory inflammatory response, which involves the participation of various leukocytes subsets, as of the monocyte/macrophage lineage. The CCR2 receptor is important to macrophage recruitment during immune responses, and play an active role in the regulation of osteoclastogenesis. Thereby, the purpose of this study was to investigate the role of CCR2+ cells in the alveolar bone repair process in mice, by means of microscopic (MicroCT, histomorphometry, birefringence analysis and immunohistochemistry) and molecular (PCRArray) comparative analysis between C57BL / 6 (WT) and CCR2KO mice during periods of 0 hour, 7, 14 and 21 days post-extraction of the right upper incisor. As a result of the microscopic analysis, we noted that the absence of CCR2+ cells did not affect in the overall outcome of alveolar bone repair in CCR2KO mice, but resulted in transient and statistically significant (p<0.05) alterations of inflammatory infiltrate, blood vessels, fibroblasts, collagen fibers, osteoblasts and osteoclasts counts. Furthermore, the absence of CCR2+cells resulted in a decrease (p<0.05) of CCR5+ and F4/80+ cells in the inflammatory infiltrate along the alveolar bone repair process in CCR2KO mice, demonstrating the role of CCR2 receptor in macrophages migration (F4/80+ cells), as well as suggesting that the F4/80+ cells are double positive for CCR2 and CCR5. In this context, CCR5 receptor could be responsible for the remaining (but reduced) migration, of the F4/80 + cells in CCR2KO mice. According to molecular results, the absence of CCR2 resulted in an altered expression of different markers in CCR2KO mice, such as: growth factor TGF1, the matrix markers COL1, MMP1a, MMP2 and MMP9, the bone markers RUNX2, DMP1 RANKL, RANK and CTSK, and MSCs markers CD106, OCT-4, NANOG, CD146 and CD105, as well as immunological markers as IL-6 and TNF-, chemokine receptors CCR1, CXCR1 and CCR5, and the chemokines CCL12, CCL20, CCL25 and CXCL12. In conclusion, these results indicate that CCR2+ cells have different functions in alveolar bone repair in mice, influencing the inflammatory response and also tissue events observed throughout the process events.

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Júnior, Antonio Carlos Tavares da Silva. « Estudo do reparo das lesões induzidas no DNA de Escherichia coli pela radiação ultravioleta C (UVC) ». Universidade do Estado do Rio de Janeiro, 2011. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=5907.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Didaticamente, podemos dividir o espectro da radiação ultravioleta (UV) em três faixas: UVA (400 a 320 nm), UVB (320 a 290 nm) e UVC (290 a 100 nm). Apesar do UVC ou UV-curto ser eficientemente filtrado pela camada de ozônio da Terra e sua atmosfera, este é uma das faixas do espectro de UV mais usadas para explorar as consequências de danos causados ao DNA, já que a letalidade induzida por este agente está relacionada aos danos diretos no genoma celular, como as lesões dímero de pirimidina, que são letais se não reparadas. Contudo, demonstrou-se que a radiação UVC pode gerar espécies reativas de oxigênio (ERO), como o oxigênio singleto (1O2). Embora, o radical hidroxil (OH) cause modificações oxidativas nas bases de DNA, alguns trabalhos indicam que o 1O2 também está envolvido nos danos oxidativos no DNA. Esta ERO é produzida por vários sistemas biológicos e reações fotossensibilização, quando cromóforos são expostos à luz visível ou são excitados pela luz UV, permitindo que essa energia possa ser transferida para o oxigênio sendo convertido em 1O2, que é conhecido por modificar resíduos de guanina, gerando 8-oxoG, que caso não seja reparada pode gerar uma transversão GC-TA. O objetivo deste trabalho foi o de elucidar a participação de ERO nos efeitos genotóxicos e mutagênicos gerados pela radiação UVC, assim como as enzimas envolvidas no processo de reparação destas lesões em células de Escherichia coli. Nos ensaios as culturas foram irradiadas com o UVC (254 nm; 15W General Electric G15T8 germicidal lamp, USA). Nossos resultados mostram que o uso de quelantes de ferro não alterou a letalidade induzida pelo UVC. A azida sódica, um captador de 1O2, protegeu as cepas contra os danos genotóxicos gerados pelo UVC e também diminuiu a frequência de mutações induzidas no teste com rifampicina. A reversão específica GC-TA foi induzida mais de 2,5 vezes no ensaio de mutagênese. A cepa deficiente na proteína de reparo Fpg, enzima que corrige a lesão 8-oxoG, apresentou menos quebras no DNA do que a cepa selvagem no ensaio de eletroforese alcalina. A letalidade induzida pelo UVC foi aumentada nos mutantes transformados com o plasmídeo pFPG, ao mesmo tempo que representou uma redução na indução mutagênica. Houve dimuição na eficiência de transformação com plasmídeo pUC 9.1 na cepa fpg quando comparado a cepa selvagem. Assim como, um aumento da sensibilidade ao UVC na associação entre mutantes fpg e uvrA. Estes resultados mostram que o 1O2 participa dos danos induzidos pelo UVC, através da geração da lesão 8-oxoG, uma lesão mutagênica, que é reparada pela proteína Fpg
Didactically, we can divide the ultraviolet radiation (UV) spectrum into three bands: UVA (400 to 320 nm), UVB (320-290 nm) and UVC (290-100 nm). Despite the UVC or far-UV be efficiently filtered by Earths ozone layer and its atmosphere, this is one of bands of UV spectrum used to explore the consequences of DNA damages, since the UVC-induced lethality is related to direct damage in genome cells, such as pyrimidine dimers, which are lethal if not repaired. However, it was shown that UVC radiation can generate reactive oxygen species (ROS) such as singlet oxygen (1O2). Although hydroxyl radical (OH) cause oxidative modifications in DNA bases, some works suggests that 1O2 is also involved in oxidative DNA damage. This ROS is produced by several biological systems and photosensitivity reactions when chromophores are exposed to visible light or excited by UV light, allowing that energy can be transferred to the oxygen being converted to 1O2, which is known to modify guanine residues, generating 8-oxoG, if not repaired can lead to a GC-TA transversion. The objective of this work was to elucidate the ROS involvement in the genotoxic and mutagenic effects generated by UVC radiation, as well as the enzymes involved in the repair process of these lesions in Escherichia coli cells. In the assays, cultures were irradiated with UVC (254 nm, 15 W General Electric germicidal lamp G15T8, USA). Our results show that the use of iron chelators did not affect the UVC-induced lethality. The sodium azide, a 1O2 quencher, protected strains against the genotoxic damage produced by UVC and also decreased the frequency of mutations induced in rifampicin assay. Reversal specific GC-TA was induced more than 2.5 fold in the mutagenesis assay. The deficient strain in the repair protein Fpg, an enzyme that corrects 8-oxoG lesions, had less DNA breakage than the wild strain in electrophoresis alkaline assay. The UVC-induced lethality was increased in mutants transformed with the pFPG plasmid, while representing a decrease in mutagenic induction. There was a reduction in the transformation efficiency with plasmid pUC 9.1 on Fpg-strain compared to wild-type strain. Also there was a lethality increase in the association between fpg and uvrA mutants. These results shows that 1O2 participates in UVC-induced damages through the generation of 8-oxoG lesion, a mutagenic lesion that is repaired by the Fpg protein.

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Pospíšil, David. « Aspekty vizualizace dat v Business Intelligence ». Master's thesis, Vysoká škola ekonomická v Praze, 2015. http://www.nusl.cz/ntk/nusl-201728.

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The thesis's primary focus is the visualization of data in Business Intelligence with broader ramifications because the framework defined here will be useful for making any design layout. It describes the cognitive aspects of perception for visualization and with this understanding the thesis will be able to summarize the rules which are necessary for right and functional design. The goal of design is to provide the information in the most understandable way. The author has surveyed individuals to find out how people are familiar with the rules for designing and the style of visualization that people prefer. The last part of this thesis is focused on the implementation of the findings with the ultimate goal to suggest a new BI reporting system. Being able to list and verify the rules of visualization on the path to implementation is the main benefit of this work.

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Hendricks, Peter Schuyler. « The early time course of smoking withdrawal symptoms ». [Tampa, Fla] : University of South Florida, 2006. http://purl.fcla.edu/usf/dc/et/SFE0001663.

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Almeida, Liliana Andreia Pereira. « Impacto das privatizações na divulgação de informação das empresas portuguesas do setor financeiro ». Master's thesis, Instituto Superior de Economia e Gestão, 2017. http://hdl.handle.net/10400.5/15183.

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Mestrado em Contabilidade, Fiscalidade e Finanças Empresariais
Este trabalho tem como objetivo analisar a evolução da divulgação de informação das empresas financeiras, em Portugal, após os processos de privatizações e fusões que ocorreram no setor durante os anos 90 do século XX. Para alcançar este objetivo foram criados índices de divulgação de informação a partir do Quadro Resumo de Indicadores e da Mensagem do Presidente contidos nos Relatórios de Gestão dos principais bancos entre 1987 e 2003. Os dados obtidos foram analisados através de métodos estatísticos recorrendo-se a estatística descritiva, a testes de associação bivariada e a modelos de regressão linear múltipla. Os resultados obtidos permitiram concluir que fatores como a natureza da propriedade, a concentração de propriedade e a presença em bolsa têm influência no grau de divulgação de informação por parte das empresas financeiras. Neste caso concreto, a alteração na natureza de propriedade com os processos de privatização ocorridos e com o aumento do número de acionistas, levou a um aumento de divulgação voluntária nos Relatórios de Gestão por parte das empresas financeiras portuguesas.
The aim of this paper is to analyze the evolution of the information disclosure of financial companies in Portugal after the privatization and merger processes that occurred in the sector in the 1990s. To achieve this objective, we created indices of information disclosure from the summary table of indicators and the message from the Chairman contained in the Management Reports of the major banks between 1987 and 2003. The data were analyzed through statistical methods using descriptive statistics, bivariate association tests and multiple linear regression models. The results allowed us to conclude that factors such as the nature of the property, the concentration of ownership and stock market presence have influence in the degree of information disclosure by financial companies. In this particular case, the change in the nature of the property with privatization processes that occurred and with the increase of the number of shareholders in the financial structures allowed an increase in voluntary disclosure in Management Reports by Portuguese financial companies.
info:eu-repo/semantics/publishedVersion

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Ricci, Tatiana Gama. « A utilização das orientações do International Integrated Reporting Council (IIRC) para relato integrado em uma empresa de saneamento municipal ». Universidade Presbiteriana Mackenzie, 2015. http://tede.mackenzie.br/jspui/handle/tede/959.

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The disclosure of information that is more than just financial by a company has become a necessity of the 21st century, and has driven the creation of several tools for communicating the social and environmental impact of companies, such as Social Balance, or Sustainability Reports. However, it has been observed that, often, the information contained within these reports is disconnected from the financial statements of the companies, which has led to discussions of integrated reporting. A group of studies was set up (an initiative of the Prince of Wales) in order to develop and provide some guidance on the integration of financial and non-financial information for the purposes of corporate reporting. Given the need for companies to integrate financial and non-financial information, to understand their impacts in other capitals, beyond the financial, and to show long-term value creation to their various stakeholders, especially capital investors, this study aimed, through an interventionist approach, to help SA Water Supply Company (SANASA) apply the guidelines presented by the International Integrated Reporting Council (IIRC) for integrated reporting, by suggesting the consolidation of the IIRC guidelines with those of the Global Reporting Initiative (GRI) in a series of phases, to be applied in the preparation of its Sustainability Report. Three of these phases were executed in this study, which serve as models for the company studied (SANASA) and can be used by other companies wishing to implement integrated reporting of financial and non-financial information according to both the IIRC guidelines and GRI guidelines. During the execution of these phases it was observed that the IIRC guidelines are broad, calling for meetings and training investments so that the company can incorporate integrated thinking into its actions, and thus result in a medium- to long-term process. The study also elaborates on the contributions, opportunities and challenges that occurred throughout the process of incorporating IIRC guidelines as they relate to the practical implementation of the integrated report and also as they differentiate the IIRC guidelines from others, facilitating the discussion about this new form of communication, which has been created to meet the specific needs of this century.
O disclosure de informações que não sejam apenas financeiras se apresenta como uma necessidade do século 21, que faz surgir algumas ferramentas de comunicação para as informações sociais e ambientais, tais como o Balanço Social, também chamado de Relatórios de Sustentabilidade. Porém foi observado que, muitas vezes, as informações constantes nesses relatórios, não se relacionavam com as demonstrações financeiras das empresas, o que fomentou a discussão a respeito do relato integrado. Assim montou-se um grupo de estudos, uma iniciativa do Príncipe de Gales, com o objetivo de fornecer orientações quanto à integração de informações financeiras e não financeiras. Nesse sentido, diante da necessidade da integração de suas informações financeiras e não financeiras, de conhecer os impactos em outros capitais, além do financeiro, e de demonstrar a criação de valor a longo prazo a seus diversos stakeholders, principalmente a seus fornecedores de capital, este trabalho buscou, através de uma abordagem intervencionista, auxiliar a empresa estudada Sociedade de Abastecimento de Água S.A. (SANASA), a utilizar as orientações do International Integrated Reporting Council (IIRC) para relato integrado, sugerindo em forma de etapas, uma consolidação das orientações do IIRC com as diretrizes da Global Reporting Initiative (GRI) para ser aplicada na elaboração do seu Relatório de Sustentabilidade. Foram aplicadas, portanto, neste trabalho três dessas etapas, que servem de modelo para a empresa estudada e que podem ser utilizados por outras empresas que pretendem iniciar o relato de suas informações financeiras e não financeiras conforme as orientações do IIRC e diretrizes da GRI. Durante a aplicação dessas etapas foi observado que as orientações do IIRC são amplas e que são necessários investimentos em treinamentos e reuniões para que a empresa consiga incorporar o pensamento integrado em suas ações, além de ser um processo de médio a longo prazo. O estudo também apresenta as contribuições, oportunidades e desafios ocorridos durante todo o processo de incorporação das orientações do IIRC o que colabora com os práticos na implantação do relato integrado e também difunde as orientações do IIRC, fomentando uma discussão quanto a essa nova forma de comunicação que tende a suprir as necessidades deste século.

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Coutinho, Carolina Chiantelli Cláudio [UNESP]. « Expressão das proteínas osteoprotegerina, RANK e RANKL durante o processo de reparo alveolar em ratos : estudo imunoistoquímico ». Universidade Estadual Paulista (UNESP), 2005. http://hdl.handle.net/11449/88947.

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Made available in DSpace on 2014-06-11T19:23:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-12-19Bitstream added on 2014-06-13T18:19:49Z : No. of bitstreams: 1 coutinho_ccc_me_araca.pdf: 114919 bytes, checksum: 49f558e8cba145ffdabb2e45dc62ada3 (MD5)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Na dinâmica da reparação óssea os fenômenos de reabsorção e neoformação são dependentes e acoplados. Proteínas efetivamente envolvidas na diferenciação celular determinam ativação ou inibição das atividades que regulam o ganho ou perda de massa óssea. Dentre as proteínas ósseas identificadas e envolvidas na dinâmica óssea podemos destacar a OPG, a RANK e RANKL como marcadores de atividades celulares. O presente trabalho tem como objetivo identificar, nos diferentes períodos da cronologia do processo de reparo alveolar através de técnica imunoistoquímica, a presença das proteínas OPG, RANK e RANKL. Para tanto foram utilizados 60 ratos machos submetidos à exodontia do incisivo superior direito e perfundidos aos 14, 21 e 28 dias pós-operatórios. As hemi-maxilas contendo o alvéolo dental em reparação foram removidas, pósfixadas, descalcificadas em EDTA, crioprotegidas e obtidos cortes longitudinais com 14æm em criostato. Os cortes foram submetidos à reação imunoistoquímica mediante a utilização de anticorpos primários para OPG, RANK e RANKL, como amplificador foi utilizado o sistema avidina-biotina e a diaminobenzidina (DAB) como cromógeno. Os resultados mostram que qualitativamente ocorre um balanço na expressão das proteínas que caracterizam reabsorção e neoformação óssea nos diferentes períodos estudados, onde aos 14 e 21 dias ocorre maior expressão de RANK. Com relação às proteínas OPG e RANKL, observa-se que elas apresentam-se expressas nas células da linhagem osteoblástica de forma similar, sendo que 28 dias é o período de maior expressão destas proteínas.
In the bone healing dynamics, the resorption and neoformation processes are dependent. Proteins involved in the cellular differentiation determinate the activation or inhibition of the activities that regulate the gain or loss of bone mass. From all of the identified bone proteins, it may be distinguished the OPG, RANK and RANKL. The present study has the aim to identify, in the different periods of alveolar bone healing chronology, the expression of OPG, RANK and RANKL proteins using the immunohistochemistry methodology. To perform this study, 60 male rats had the right upper incisive extracted and they were perfused at 14, 21 and 28 pos-operative days. The hemimaxilla with the rat extraction socket was removed, pos fixed and decalcified in EDTA. Then, they were cryoprotected and longitudinal slices with 14 ìm thickness were obtained in cryostat. The slices were submitted to immunohistochemistry reaction and the primary antibodies used were against OPG, RANK and RANKL proteins. It was used the avidinbiotin system to amplify the sign and diaminobenzidine was the cromogen. The results show that there is a balance in the expression of the proteins, showing that there is an increase in the expression of RANK at 14 and 21 pos-operative periods. In relation to OPG and RANKL, these proteins presents a similar expression in all of the pos-extraction periods analysed in this study and at 28 days after the extraction there is the greater expression of both proteins.

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Coutinho, Carolina Chiantelli Cláudio. « Expressão das proteínas osteoprotegerina, RANK e RANKL durante o processo de reparo alveolar em ratos : estudo imunoistoquímico / ». Araçatuba, 2005. http://hdl.handle.net/11449/88947.

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Resumo: Na dinâmica da reparação óssea os fenômenos de reabsorção e neoformação são dependentes e acoplados. Proteínas efetivamente envolvidas na diferenciação celular determinam ativação ou inibição das atividades que regulam o ganho ou perda de massa óssea. Dentre as proteínas ósseas identificadas e envolvidas na dinâmica óssea podemos destacar a OPG, a RANK e RANKL como marcadores de atividades celulares. O presente trabalho tem como objetivo identificar, nos diferentes períodos da cronologia do processo de reparo alveolar através de técnica imunoistoquímica, a presença das proteínas OPG, RANK e RANKL. Para tanto foram utilizados 60 ratos machos submetidos à exodontia do incisivo superior direito e perfundidos aos 14, 21 e 28 dias pós-operatórios. As hemi-maxilas contendo o alvéolo dental em reparação foram removidas, pósfixadas, descalcificadas em EDTA, crioprotegidas e obtidos cortes longitudinais com 14æm em criostato. Os cortes foram submetidos à reação imunoistoquímica mediante a utilização de anticorpos primários para OPG, RANK e RANKL, como amplificador foi utilizado o sistema avidina-biotina e a diaminobenzidina (DAB) como cromógeno. Os resultados mostram que qualitativamente ocorre um balanço na expressão das proteínas que caracterizam reabsorção e neoformação óssea nos diferentes períodos estudados, onde aos 14 e 21 dias ocorre maior expressão de RANK. Com relação às proteínas OPG e RANKL, observa-se que elas apresentam-se expressas nas células da linhagem osteoblástica de forma similar, sendo que 28 dias é o período de maior expressão destas proteínas.
Abstract: In the bone healing dynamics, the resorption and neoformation processes are dependent. Proteins involved in the cellular differentiation determinate the activation or inhibition of the activities that regulate the gain or loss of bone mass. From all of the identified bone proteins, it may be distinguished the OPG, RANK and RANKL. The present study has the aim to identify, in the different periods of alveolar bone healing chronology, the expression of OPG, RANK and RANKL proteins using the immunohistochemistry methodology. To perform this study, 60 male rats had the right upper incisive extracted and they were perfused at 14, 21 and 28 pos-operative days. The hemimaxilla with the rat extraction socket was removed, pos fixed and decalcified in EDTA. Then, they were cryoprotected and longitudinal slices with 14 ìm thickness were obtained in cryostat. The slices were submitted to immunohistochemistry reaction and the primary antibodies used were against OPG, RANK and RANKL proteins. It was used the avidinbiotin system to amplify the sign and diaminobenzidine was the cromogen. The results show that there is a balance in the expression of the proteins, showing that there is an increase in the expression of RANK at 14 and 21 pos-operative periods. In relation to OPG and RANKL, these proteins presents a similar expression in all of the pos-extraction periods analysed in this study and at 28 days after the extraction there is the greater expression of both proteins.
Orientador: Roberta Okamoto
Coorientador: Roelf Justino Cruz Rizollo
Banca: Idelmo Rangel Garcia Júnior
Banca: Victor Elias Arana-Chavez
Mestre

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Almeida, Terezinha Aparecida de. « Efeito dos lasers vermelho e infravermelho sobre a ativação das células responsáveis pelo direcionamento do reparo muscular ». Universidade Nove de Julho, 2015. http://bibliotecatede.uninove.br/handle/tede/1819.

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Interactions between the muscle and macrophages which invade it after an injury occurs are crucial to the evolution of repair in this tissue. On the other hand, low intensity laser (LLLT) has long been used in the treatment of such lesions, both in clinical and experimental settings; however, little is known about its effects on macrophages. The objective of this project was to evaluate the effect of irradiation with red and infrared laser on the activation state of M1 and M2a phenotypes of macrophages. For this purpose, J774 macrophage cultures were treated with polarizing agents (LPS and IFN-y or IL-4) for 24 hours and were then irradiated with 2 wavelengths of LLLT – 780 nm and 660 nm – in the same dosimetric parameters (70mW; 17.5J/cm2; 0.8J). After 24 hours of incubation, the activation state was checked by MTT technique. In each experimental situation, non-irradiated cells served as controls, and three independent experiments were performed. Laser irradiation with 780 nm proved able to decrease the activation of macrophages polarized for M1 phenotype and increase activation of M2a profile macrophages. Laser irradiation with 660 nm slightly intensified the state and activation of M1 macrophages, while significantly increasing the activation state of M2a profile macrophages. Although it is not possible to establish a relationship between the results of in vitro studies and future clinical outcomes, and the effects of laser irradiation on other macrophage phenotypes still need to be assessed, data from this study suggest that red and infrared laser could have different results when used in the different stages of muscle repair.
As interações entre o músculo e os macrófagos que o invadem após a ocorrência da lesão são determinantes para a evolução do reparo deste tecido. Por outro lado, o laser em baixa intensidade (LBI) tem sido muito utilizado no tratamento destas lesões no âmbito clínico e no experimental, mas pouco se conhece a respeito dos seus efeitos sobre os macrófagos. O objetivo deste projeto foi avaliar o efeito da irradiação com laser vermelho e infravermelho sobre o estado de ativação de macrófagos de fenótipo M1 e M2a. Para tanto, culturas de macrófagos J774 foram tratadas com agentes polarizadores (LPS e IFN-y ou IL-4) por 24h e então irradiadas com LBI em 2 comprimentos de onda 780nm e 660nm nos mesmos parâmetros dosimétricos (70mW; 17,5J/cm2; 0,8J). Após 24h de incubação, o estado de ativação foi verificado por meio da técnica MTT. Em cada situação experimental, células não irradiadas serviram como controle, sendo realizados três experimentos independentes. A irradiação com laser de 780 nm mostrou-se capaz de diminuir a ativação de macrófagos polarizados para fenótipo M1 e aumentar a ativação de macrófagos de perfil M2a. Já a irradiação com laser de 660 nm intensificou ligeiramente o estado e ativação dos macrófagos M1 e aumentou de maneira significante o estado de ativação de macrófagos de perfil M2a. Embora não se possa estabelecer uma relação entre resultados de estudos in vitro e desfechos clínicos futuros e ainda exista necessidade de avaliar os efeitos da irradiação laser nos outros fenótipos de macrófagos, os dados do presente estudo sugerem que os lasers vermelho e infravermelho pode trazer resultados diferentes quando utilizados nas diferentes fases do reparo muscular.

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Manda, David. « Forenzní analýza v operačních systémech Windows ». Master's thesis, Vysoké učení technické v Brně. Ústav soudního inženýrství, 2016. http://www.nusl.cz/ntk/nusl-241258.

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The thesis is focused on digital forensic analysis in operating system Windows. The purpose of this thesis is to provide a preview to the techniques and procedures forensics analysis. There are disclosed available software tools used during forensic analysis. The part of this thesis is also practical process of forensic analysis and developing forensic report

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