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Articles de revues sur le sujet « Cyclomodulin »

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Auteur : Grafiati
Publié le 6 juillet 2024

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1

Markelova, Natalia N., Elena F. Semenova, Olga N. Sineva et Vera S. Sadykova. « The Role of Cyclomodulins and Some Microbial Metabolites in Bacterial Microecology and Macroorganism Carcinogenesis ». International Journal of Molecular Sciences 23, no 19 (3 octobre 2022) : 11706. http://dx.doi.org/10.3390/ijms231911706.

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A number of bacteria that colonize the human body produce toxins and effectors that cause changes in the eukaryotic cell cycle—cyclomodulins and low-molecular-weight compounds such as butyrate, lactic acid, and secondary bile acids. Cyclomodulins and metabolites are necessary for bacteria as adaptation factors—which are influenced by direct selection—to the ecological niches of the host. In the process of establishing two-way communication with the macroorganism, these compounds cause limited damage to the host, despite their ability to disrupt key processes in eukaryotic cells, which can lead to pathological changes. Possible negative consequences of cyclomodulin and metabolite actions include their potential role in carcinogenesis, in particular, with the ability to cause DNA damage, increase genome instability, and interfere with cancer-associated regulatory pathways. In this review, we aim to examine cyclomodulins and bacterial metabolites as important factors in bacterial survival and interaction with the host organism to show their heterogeneous effect on oncogenesis depending on the surrounding microenvironment, pathological conditions, and host genetic background.
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Nouri, Roghayeh, Alka Hasani, Kourosh Masnadi Shirazi, Mohammad Reza Alivand, Bita Sepehri, Simin Sotoudeh, Fatemeh Hemmati, Afshin Fattahzadeh, Babak Abdinia et Mohammad Ahangarzadeh Rezaee. « Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects : Variations in the Prevalence and Attributing Features ». Canadian Journal of Infectious Diseases and Medical Microbiology 2021 (9 novembre 2021) : 1–8. http://dx.doi.org/10.1155/2021/2131787.

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Accumulating evidence indicates that specific strains of mucosa-associated Escherichia coli (E. coli) can influence the development of colorectal carcinoma. This study aimed to investigate the prevalence and characterization of mucosa-associated E. coli obtained from the colorectal cancer (CRC) patients and control group. At two referral university-affiliated hospitals in northwest Iran, 100 patients, 50 with CRC and 50 without, were studied over the course of a year. Fresh biopsy specimens were used to identify mucosa-associated E. coli isolates after dithiothreitol mucolysis. To classify the E. coli strains, ten colonies per sample were typed using enterobacterial repetitive intergenic consensus-based PCR (ERIC-PCR). The strains were classified into phylogroups using the quadruplex PCR method. The PCR method was used to examine for the presence of cyclomodulin, bfp, stx1, stx2, and eae-encoding genes. The strains were tested for biofilm formation using the microtiter plate assay. CRC patients had more mucosa-associated E. coli than the control group ( p < 0.05 ). Enteropathogenic Escherichia coli (EPEC) was also found in 23% of CRC strains and 7.1% of control strains ( p < 0.05 ). Phylogroup A was predominant in control group specimens, while E. coli isolates from CRC patients belonged most frequently to phylogroups D and B2. Furthermore, the frequency of cyclomodulin-encoding genes in the CRC patients was significantly higher than the control group. Around 36.9% of E. coli strains from CRC samples were able to form biofilms, compared to 16.6% E. coli strains from the control group ( p < 0.05 ). Noticeably, cyclomodulin-positive strains were more likely to form biofilm in comparison to cyclomodulin-negative strains ( p < 0.05 ). In conclusion, mucosa-associated E. coli especially cyclomodulin-positive isolates from B2 and D phylogroups possessing biofilm-producing capacity colonize the gut mucosa of CRC patients.
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Mezerová, Kristýna, Lubomír Starý, Pavel Zbořil, Ivo Klementa, Martin Stašek, Petr Špička, Pavel Skalický et Vladislav Raclavský. « Cyclomodulins and Hemolysis in E. coli as Potential Low-Cost Non-Invasive Biomarkers for Colorectal Cancer Screening ». Life 11, no 11 (31 octobre 2021) : 1165. http://dx.doi.org/10.3390/life11111165.

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The frequent occurrence of E. coli positive for cyclomodulins such as colibactin (CLB), the cytotoxic necrotizing factor (CNF), and the cytolethal distending factor (CDT) in colorectal cancer (CRC) patients published so far provides the opportunity to use them as CRC screening markers. We examined the practicability and performance of a low-cost detection approach that relied on culture followed by simplified DNA extraction and PCR in E. coli isolates recovered from 130 CRC patients and 111 controls. Our results showed a statistically significant association between CRC and the presence of colibactin genes clbB and clbN, the cnf gene, and newly, the hemolytic phenotype of E. coli isolates. We also observed a significant increase in the mean number of morphologically distinct E. coli isolates per patient in the CRC cohort compared to controls, indicating that the cyclomodulin-producing E. coli strains may represent potentially preventable harmful newcomers in CRC patients. A colibactin gene assay showed the highest detection rate (45.4%), and males would benefit from the screening more than females. However, because of the high number of false positives, practical use of this marker must be explored. In our opinion, it may serve as an auxiliary marker to increase the specificity and/or sensitivity of the well-established fecal immunochemical test (FIT) in CRC screening.
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Samba-Louaka, Ascel, Jean-Philippe Nougayrède, Claude Watrin, Eric Oswald et Frédéric Taieb. « The Enteropathogenic Escherichia coli Effector Cif Induces Delayed Apoptosis in Epithelial Cells ». Infection and Immunity 77, no 12 (28 septembre 2009) : 5471–77. http://dx.doi.org/10.1128/iai.00860-09.

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ABSTRACT The cycle inhibiting factor (Cif) belongs to a family of bacterial toxins, the cyclomodulins, which modulate the host cell cycle. Upon injection into the host cell by the type III secretion system of enteropathogenic Escherichia coli (EPEC), Cif induces both G2 and G1 cell cycle arrests. The cell cycle arrests correlate with the accumulation of p21waf1 and p27kip1 proteins that inhibit CDK-cyclin complexes, whose activation is required for G1/S and G2/M transitions. Increases of p21 and p27 levels are independent of p53 transcriptional induction and result from protein stabilization through inhibition of the ubiquitin/proteasome degradation pathway. In this study, we show that Cif not only induces cell cycle arrest but also eventually provokes a delayed cell death. Indeed, 48 h after infection with EPEC expressing Cif, cultured IEC-6 intestinal cells were positive for extracellular binding of annexin V and exhibited high levels of cleaved caspase-3 and lactate dehydrogenase release, indicating evidence of apoptosis. Cif was necessary and sufficient for inducing this late apoptosis, and the cysteine residue of the catalytic site was required for Cif activity. These results highlight a more complex role of Cif than previously thought, as a cyclomodulin but also as an apoptosis inducer.
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Hsu, Yun, Gregory Jubelin, Frédéric Taieb, Jean-Philippe Nougayrède, Eric Oswald et C. Erec Stebbins. « Structure of the Cyclomodulin Cif from Pathogenic Escherichia coli ». Journal of Molecular Biology 384, no 2 (décembre 2008) : 465–77. http://dx.doi.org/10.1016/j.jmb.2008.09.051.

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Canizalez-Roman, Adrian, Juan E. Reina-Reyes, Uriel A. Angulo-Zamudio, Eloy E. Geminiano-Martínez, Antonio F. Flores-Carrillo, Rolando R. García-Matus, Norma M. Valencia-Mijares et al. « Prevalence of Cyclomodulin-Positive E. coli and Klebsiella spp. Strains in Mexican Patients with Colon Diseases and Antimicrobial Resistance ». Pathogens 11, no 1 (23 décembre 2021) : 14. http://dx.doi.org/10.3390/pathogens11010014.

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Colon diseases, such as colorectal cancer (CRC), are multifactor diseases that affect more than one million people per year; recently, the microbiota has been associated with an etiologic factor, specifically bacterial cyclomodulin positivity (CM+). Unfortunately, there are no studies from Mexico that detail the presence of bacterial CM+ in patients with colon diseases. We therefore performed a comprehensive study to investigate the associations and prevalence of cyclomodulin-positive Diarrheagenic E. coli (DEC), non-DEC, and Klebsiella spp. strains isolated from Mexican subjects with colon diseases. In this work, we analyzed 43 biopsies, 87 different bacteria were isolated, and E. coli was the most frequently noted, followed by Klebsiella spp., and Enterococcus spp. E. coli, non-DEC, and EPEC belonging to phylogroup B2 were the most prevalent. More than 80% of E. coli and Klebsiella were CM+. pks, cdt, cnf, and cif were identified. cdt was associated with non-DEC, cif and its combinations with EPEC, as well as cdt and psk with Klebsiella. Lastly, all the CM+ bacteria were resistant to at least one antibiotic (34% were MDR, and 48% XDR). In conclusion, the high prevalence of bacterial CM+ in colon disease patients suggests that these bacteria play an important role in the genesis of these diseases.
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Toro, Tasha B., Julia I. Toth et Matthew D. Petroski. « The Cyclomodulin Cycle Inhibiting Factor (CIF) Alters Cullin Neddylation Dynamics ». Journal of Biological Chemistry 288, no 21 (15 avril 2013) : 14716–26. http://dx.doi.org/10.1074/jbc.m112.448258.

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Buc, Emmanuel, Damien Dubois, Pierre Sauvanet, Jennifer Raisch, Julien Delmas, Arlette Darfeuille-Michaud, Denis Pezet et Richard Bonnet. « High Prevalence of Mucosa-Associated E. coli Producing Cyclomodulin and Genotoxin in Colon Cancer ». PLoS ONE 8, no 2 (14 février 2013) : e56964. http://dx.doi.org/10.1371/journal.pone.0056964.

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Chavez, Carolina Varela, Grégory Jubelin, Gabriel Courties, Aurélie Gomard, Nadège Ginibre, Sylvie Pages, Frédéric Taïeb et al. « The cyclomodulin Cif of Photorhabdus luminescens inhibits insect cell proliferation and triggers host cell death by apoptosis ». Microbes and Infection 12, no 14-15 (décembre 2010) : 1208–18. http://dx.doi.org/10.1016/j.micinf.2010.09.006.

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Samba-Louaka, Ascel, Jean-Philippe Nougayrède, Claude Watrin, Grégory Jubelin, Eric Oswald et Frédéric Taieb. « Bacterial cyclomodulin Cif blocks the host cell cycle by stabilizing the cyclin-dependent kinase inhibitors p21waf1and p27kip1 ». Cellular Microbiology 10, no 12 (décembre 2008) : 2496–508. http://dx.doi.org/10.1111/j.1462-5822.2008.01224.x.

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Herrera-Vázquez, Arturo, Rebeca Arellano-Aranda, Daniel Hernández-Cueto, Esmeralda Rodríguez-Miranda, Sergio López-Briones et Marco Antonio Hernández-Luna. « Detection of Cyclomodulin CNF-1 Toxin-Producing Strains of Escherichia coli in Pig Kidneys at a Slaughterhouse ». Microorganisms 11, no 8 (11 août 2023) : 2065. http://dx.doi.org/10.3390/microorganisms11082065.

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Food is often contaminated with Escherichia coli (E. coli) bacteria strains, which have been associated with different diseases, including urinary tract infections. The consumption of meat by humans is a potential route of transmission of antimicrobial resistance, and food-producing animals have been associated as a major reservoir of resistant bacterial strains. The aim of this study was to determine the presence of the E. coli strains producing the CNF-1 toxin in pig kidneys. Pig kidneys were collected from a Mexican slaughterhouse and classified according to their coloration into reddish kidneys (RK) and yellowish kidneys (YK). A tissue sample from each kidney was processed for histological analysis, the presence of E. coli was determined by conventional PCR assay, and the CNF-1 toxin was detected by both conventional PCR and Western blotting. Herein, an inflammatory cell infiltrate was found in all collected kidneys, regardless of macroscopic differences. Surprisingly, E. coli and the CNF-1 toxin were detected in all kidney samples. We clearly demonstrate contamination by CNF-1 toxin-producing E. coli in pork kidneys from a slaughterhouse, even in those without apparent damage. This suggests that pork may serve as a reservoir for pathogens, representing an important risk to human health.
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Patel, Hiren G., Seme Tabassum et Sohail Shaikh. « E. coli Sepsis : Red Flag for Colon Carcinoma—A Case Report and Review of the Literature ». Case Reports in Gastrointestinal Medicine 2017 (2017) : 1–3. http://dx.doi.org/10.1155/2017/2570524.

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We present an atypical case of newly diagnosed colon cancer and provide insight into the infectious predispositions of E. coli bacteremia to the development of colon adenocarcinoma. A 66-year-old female was admitted to the hospital with one-week symptoms of subjective fevers, chills, and lower back pain. Upon initial evaluation, her temperature was 101 degrees Fahrenheit with a white count of 12,000 K/mm3. Initial septic workup was positive for E. coli bacteremia. The patient was started on Aztreonam. Repeat blood culture 48 hours later was negative for any growth. However, later during hospital stay blood culture was repeated due to SIRS, which was positive again for E. coli. CT scan of the chest/abdomen/pelvis with contrast revealed no signs of colitis. Without clear etiology for recurrent E. coli bacteremia ultimately colonoscopy was performed which showed an ulcerated mass in the cecum. Biopsy showed moderately differentiated adenocarcinoma. E. coli strains B2 and D produce cyclomodulin toxins as part of their virulence, which interferes with the cell cycle regulation, promoting chromosomal instability, and increasing susceptibility to cancer. In patients with recurrent E. coli bacteremia with an unknown source, colonoscopy should be done to look for colon cancer.
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Taieb, Frédéric, Jean-Philippe Nougayrède, Claude Watrin, Ascel Samba-Louaka et Eric Oswald. « Escherichia coli cyclomodulin Cif induces G2arrest of the host cell cycle without activation of the DNA-damage checkpoint-signalling pathway ». Cellular Microbiology 8, no 12 (décembre 2006) : 1910–21. http://dx.doi.org/10.1111/j.1462-5822.2006.00757.x.

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Putze, Johannes, Claire Hennequin, Jean-Philippe Nougayrède, Wenlan Zhang, Stefan Homburg, Helge Karch, Marie-Agnés Bringer et al. « Genetic Structure and Distribution of the Colibactin Genomic Island among Members of the Family Enterobacteriaceae ». Infection and Immunity 77, no 11 (31 août 2009) : 4696–703. http://dx.doi.org/10.1128/iai.00522-09.

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ABSTRACT A genomic island encoding the biosynthesis and secretion pathway of putative hybrid nonribosomal peptide-polyketide colibactin has been recently described in Escherichia coli. Colibactin acts as a cyclomodulin and blocks the eukaryotic cell cycle. The origin and prevalence of the colibactin island among enterobacteria are unknown. We therefore screened 1,565 isolates of different genera and species related to the Enterobacteriaceae by PCR for the presence of this DNA element. The island was detected not only in E. coli but also in Klebsiella pneumoniae, Enterobacter aerogenes, and Citrobacter koseri isolates. It was highly conserved among these species and was always associated with the yersiniabactin determinant. Structural variations between individual strains were only observed in an intergenic region containing variable numbers of tandem repeats. In E. coli, the colibactin island was usually restricted to isolates of phylogenetic group B2 and inserted at the asnW tRNA locus. Interestingly, in K. pneumoniae, E. aerogenes, C. koseri, and three E. coli strains of phylogenetic group B1, the functional colibactin determinant was associated with a genetic element similar to the integrative and conjugative elements ICEEc1 and ICEKp1 and to several enterobacterial plasmids. Different asn tRNA genes served as chromosomal insertion sites of the ICE-associated colibactin determinant: asnU in the three E. coli strains of ECOR group B1, and different asn tRNA loci in K. pneumoniae. The detection of the colibactin genes associated with an ICE-like element in several enterobacteria provides new insights into the spread of this gene cluster and its putative mode of transfer. Our results shed light on the mechanisms of genetic exchange between members of the family Enterobacteriaceae.
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Dubois, D., J. Delmas, A. Cady, F. Robin, A. Sivignon, E. Oswald et R. Bonnet. « Cyclomodulins in Urosepsis Strains of Escherichia coli ». Journal of Clinical Microbiology 48, no 6 (7 avril 2010) : 2122–29. http://dx.doi.org/10.1128/jcm.02365-09.

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Nougayrède, Jean-Philippe, Frédéric Taieb, Jean De Rycke et Eric Oswald. « Cyclomodulins : bacterial effectors that modulate the eukaryotic cell cycle ». Trends in Microbiology 13, no 3 (mars 2005) : 103–10. http://dx.doi.org/10.1016/j.tim.2005.01.002.

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Taieb, Frédéric, Jean-Philippe Nougayrède et Eric Oswald. « Cycle Inhibiting Factors (Cifs) : Cyclomodulins That Usurp the Ubiquitin-Dependent Degradation Pathway of Host Cells ». Toxins 3, no 4 (29 mars 2011) : 356–68. http://dx.doi.org/10.3390/toxins3040356.

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Taieb, Frederic, Domonkos Sváb, Claude Watrin, Eric Oswald et István Tóth. « Cytolethal distending toxin A, B and C subunit proteins are necessary for the genotoxic effect of Escherichia coli CDT-V ». Acta Veterinaria Hungarica 63, no 1 (1 mars 2015) : 1–10. http://dx.doi.org/10.1556/avet.2015.001.

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Cytolethal distending toxins (CDT) are considered the prototype of inhibitory cyclomodulins, and are produced by a wide range of Gram-negative pathogenic bacteria, includingEscherichia colistrains of various sero- and pathotypes. CDT is a heterotripartite toxin consisting of three protein subunits, CdtA, CdtB and CdtC. The active subunit, CdtB has DNase activity and causes DNA damage and cell cycle arrest in the target cell. However, several studies have highlighted different roles for CdtA and CdtC subunits. In order to reveal the necessity of CdtA and CdtC subunit proteins in the CDT-specific phenotype, expression clones containing thecdt-Vsubunit genes were constructed. Using cell culture assays, we demonstrated that clones expressing only the CdtB subunit or in combination with only CdtA or CdtC were unable to trigger the specific cell cycle arrest and changes in cell morphology in HeLa cells. At the same time, the recombinant clone harbouring the wholecdt-Voperon caused all the CDT-associated characteristic phenotypes. All these results verify that all the three CDT subunit proteins are necessary for the genotoxic effect caused by CDT-V.
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Jubelin, Grégory, Carolina Varela Chavez, Frédéric Taieb, Mark J. Banfield, Ascel Samba-Louaka, Rika Nobe, Jean-Philippe Nougayrède et al. « Cycle Inhibiting Factors (CIFs) Are a Growing Family of Functional Cyclomodulins Present in Invertebrate and Mammal Bacterial Pathogens ». PLoS ONE 4, no 3 (24 mars 2009) : e4855. http://dx.doi.org/10.1371/journal.pone.0004855.

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Shima, Ayaka, Atsushi Hinenoya, Masahiro Asakura, Norihiko Sugimoto, Teizo Tsukamoto, Hideaki Ito, Akira Nagita, Shah M. Faruque et Shinji Yamasaki. « Molecular Characterizations of Cytolethal Distending Toxin Produced by Providencia alcalifaciens Strains Isolated from Patients with Diarrhea ». Infection and Immunity 80, no 4 (17 janvier 2012) : 1323–32. http://dx.doi.org/10.1128/iai.05831-11.

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ABSTRACTCytolethal distending toxins (CDTs), which block eukaryotic cell proliferation by acting as inhibitory cyclomodulins, are produced by diverse groups of Gram-negative bacteria. Active CDT is composed of three polypeptides—CdtA, CdtB, and CdtC—encoded by the genescdtA,cdtB, andcdtC, respectively. We developed a PCR-restriction fragment length polymorphism assay for the detection and differentiation of five alleles ofcdtB(Cdt-I through Cdt-V) inEscherichia coliand used the assay to investigate the prevalence and characteristic of CDT-producingE. coliin children with diarrhea (A. Hinenoya et al., Microbiol. Immunol. 53:206–215, 2009). In these assays, two untypablecdtBgenes were detected and the organisms harboring thecdtBgene were identified asProvidencia alcalifaciens(strains AH-31 and AS-1). Nucleotide sequence analysis of thecdtgene cluster revealed that thecdtA,cdtB, andcdtCgenes ofP. alcalifaciensare of 750, 810, and 549 bp, respectively. To understand the possible horizontal transfer of thecdtgenes among closely related species, the presence ofcdtgenes was screened in variousProvidenciaspp. by colony hybridization assay, and thecdtgene cluster was found in only limited strains ofP. alcalifaciens. Genome walking revealed that thecdtgene cluster ofP. alcalifaciensis located adjacent to a putative transposase gene, suggesting the locus might be horizontally transferable. Interestingly, the CDT ofP. alcalifaciens(PaCDT) showed some homology with the CDT ofShigella boydii. Whereas filter-sterilized lysates of strains AH-31 and AS-1 showed distention of CHO but not of HeLa cells,E. coliCDT-I exhibited distention of both cells. This activity of PaCDT was confirmed by generating recombinant PaCDT protein, which could also be neutralized by rabbit anti-PaCdtB antibody. Furthermore, recombinant PaCDT was found to induce G2/M cell cycle arrest and phosphorylation of host histone H2AX, a sensitive marker of DNA double-strand breaks. To our knowledge, this is the first report showing that certain clinicalP. alcalifaciensstrains could produce variants of the CDTs compared.
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McCoy, Colleen S., Anthony J. Mannion, Yan Feng, Carolyn M. Madden, Stephen C. Artim, Gina G. Au, Mikayla Dolan et al. « Cytotoxic Escherichia coli strains encoding colibactin, cytotoxic necrotizing factor, and cytolethal distending toxin colonize laboratory common marmosets (Callithrix jacchus) ». Scientific Reports 11, no 1 (27 janvier 2021). http://dx.doi.org/10.1038/s41598-020-80000-1.

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AbstractCyclomodulins are virulence factors that modulate cellular differentiation, apoptosis, and proliferation. These include colibactin (pks), cytotoxic necrotizing factor (cnf), and cytolethal distending toxin (cdt). Pathogenic pks+, cnf+, and cdt+ E. coli strains are associated with inflammatory bowel disease (IBD) and colorectal cancer in humans and animals. Captive marmosets are frequently afflicted with IBD-like disease, and its association with cyclomodulins is unknown. Cyclomodulin-encoding E. coli rectal isolates were characterized using PCR-based assays in healthy and clinically affected marmosets originating from three different captive sources. 139 E. coli isolates were cultured from 122 of 143 marmosets. The pks gene was detected in 56 isolates (40%), cnf in 47 isolates (34%), and cdt in 1 isolate (0.7%). The prevalences of pks+ and cnf+ E. coli isolates were significantly different between the three marmoset colonies. 98% of cyclomodulin-positive E. coli belonged to phylogenetic group B2. Representative isolates demonstrated cyclomodulin cytotoxicity, and serotyping and whole genome sequencing were consistent with pathogenic E. coli strains. However, the presence of pks+, cnf+, or cdt+ E. coli did not correlate with clinical gastrointestinal disease in marmosets. Cyclomodulin-encoding E. coli colonize laboratory common marmosets in a manner dependent on the source, potentially impacting reproducibility in marmoset models.
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Kamali Dolatabadi, Razie, Hossein Fazeli, Mohammad Hassan Emami, Vajihe Karbasizade, Fatemeh Maghool, Alireza Fahim et Hojatollah Rahimi. « Phenotypicand Genotypic Characterization of Clinical Isolates of Intracellular Adherent–Invasive Escherichia coli Among Different Stages, Family History, and Treated Colorectal Cancer Patients in Iran ». Frontiers in Cellular and Infection Microbiology 12 (11 juillet 2022). http://dx.doi.org/10.3389/fcimb.2022.938477.

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There is increasing evidence showing that microbial dysbiosis impacts the health and cancer risk of the host. An association between adherent–invasive Escherichia coli (AIEC) and colorectal cancer (CRC) has been revealed. Cyclomodulins (CMs) have been receiving increasing attention for carcinogenic changes. In this study, the incidence and features of intracellular AIEC and cyclomodulin-encoding genes were investigated and the phylogenetic grouping and genetic relatedness were evaluated. E. coli strains were isolated from the colorectal biopsies. Adhesion and invasion assays and intramacrophage cell survival test were performed to separate the AIEC isolates. Virulence genotyping for the genes htrA, dsbA, chuA, and lpfA and the cyclomodulin toxins was also conducted. In addition, phylogenetic grouping of the isolates was determined. Subsequently, repetitive element sequence-based PCR (rep-PCR) fingerprinting was performed. A total of 24 AIEC pathovars were isolated from 150 patients. The prevalence rates of htr, dsbA, and lpfA were 70.83% and that of chuA was 91.66%. The frequencies of the cyclomodulin toxins were as follows: cnf1, 29.2%; cnf2, 25%; colibactin, 29.2%; and cdt, 4.2%; cif was not found. Among the AIEC isolates, 4.2%, 4.2%, 54.2%, 29.2%, and 8.3% with phylotypes A or C, B1, B2, D, and E were identified, respectively. Left-sided colon carcinoma and adenocarcinoma T≥1 stage (CRC2) were colonized by B2 phylogroup AIEC-producing CMs more often than the samples from the other groups. Close genetic relatedness was observed in AIEC isolates with rep-PCR.
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Mambu, Julien, Emilie Barilleau, Laetitia Fragnet-Trapp, Yves Le Vern, Michel Olivier, Guillaume Sadrin, Olivier Grépinet, Frédéric Taieb, Philippe Velge et Agnès Wiedemann. « Rck of Salmonella Typhimurium Delays the Host Cell Cycle to Facilitate Bacterial Invasion ». Frontiers in Cellular and Infection Microbiology 10 (2 novembre 2020). http://dx.doi.org/10.3389/fcimb.2020.586934.

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Salmonella Typhimurium expresses on its outer membrane the protein Rck which interacts with the epidermal growth factor receptor (EGFR) of the plasma membrane of the targeted host cells. This interaction activates signaling pathways, leading to the internalization of Salmonella. Since EGFR plays a key role in cell proliferation, we sought to determine the influence of Rck mediated infection on the host cell cycle. By analyzing the DNA content of uninfected and infected cells using flow cytometry, we showed that the Rck-mediated infection induced a delay in the S-phase (DNA replication phase) of the host cell cycle, independently of bacterial internalization. We also established that this Rck-dependent delay in cell cycle progression was accompanied by an increased level of host DNA double strand breaks and activation of the DNA damage response. Finally, we demonstrated that the S-phase environment facilitated Rck-mediated bacterial internalization. Consequently, our results suggest that Rck can be considered as a cyclomodulin with a genotoxic activity.
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Zhang, Zhen, Kyaw Min Aung, Bernt Eric Uhlin et Sun Nyunt Wai. « Reversible senescence of human colon cancer cells after blockage of mitosis/cytokinesis caused by the CNF1 cyclomodulin from Escherichia coli ». Scientific Reports 8, no 1 (décembre 2018). http://dx.doi.org/10.1038/s41598-018-36036-5.

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Morgan, Radwa N., Sarra E. Saleh, Hala A. Farrag et Mohammad M. Aboulwafa. « Bacterial cyclomodulins : types and roles in carcinogenesis ». Critical Reviews in Microbiology, 15 juillet 2021, 1–25. http://dx.doi.org/10.1080/1040841x.2021.1944052.

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El-Aouar Filho, Rachid A., Aurélie Nicolas, Thiago L. De Paula Castro, Martine Deplanche, Vasco A. De Carvalho Azevedo, Pierre L. Goossens, Frédéric Taieb, Gerard Lina, Yves Le Loir et Nadia Berkova. « Heterogeneous Family of Cyclomodulins : Smart Weapons That Allow Bacteria to Hijack the Eukaryotic Cell Cycle and Promote Infections ». Frontiers in Cellular and Infection Microbiology 7 (23 mai 2017). http://dx.doi.org/10.3389/fcimb.2017.00208.

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DeLira-Bustillos, Nora, Uriel A. Angulo-Zamudio, Nidia Leon-Sicairos, Hector Flores-Villaseñor, Jorge Velazquez-Roman, Gabriela Tapia-Pastrana, Francisco A. Martínez-Villa et al. « Distribution and virulence of Escherichia coli harboring cyclomodulins and supplementary virulence genes isolates from clinical and environmental samples ». Microbial Pathogenesis, mars 2024, 106634. http://dx.doi.org/10.1016/j.micpath.2024.106634.

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El-Aouar Filho, Rachid A., Aurélie Nicolas, Thiago L. De Paula Castro, Martine Deplanche, Vasco A. De Carvalho Azevedo, Pierre L. Goossens, Frédéric Taieb, Gerard Lina, Yves Le Loir et Nadia Berkova. « Corrigendum : Heterogeneous Family of Cyclomodulins : Smart Weapons That Allow Bacteria to Hijack the Eukaryotic Cell Cycle and Promote Infections ». Frontiers in Cellular and Infection Microbiology 7 (14 août 2017). http://dx.doi.org/10.3389/fcimb.2017.00364.

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