Bibliographies thématiques / Cryo-CLEM

Littérature scientifique sur le sujet « Cryo-CLEM »

Auteur : Grafiati
Publié le 14 septembre 2024

Créez une référence correcte selon les styles APA, MLA, Chicago, Harvard et plusieurs autres

Choisissez une source :

Sommaire

Consultez les listes thématiques d’articles de revues, de livres, de thèses, de rapports de conférences et d’autres sources académiques sur le sujet « Cryo-CLEM ».

À côté de chaque source dans la liste de références il y a un bouton « Ajouter à la bibliographie ». Cliquez sur ce bouton, et nous générerons automatiquement la référence bibliographique pour la source choisie selon votre style de citation préféré : APA, MLA, Harvard, Vancouver, Chicago, etc.

Vous pouvez aussi télécharger le texte intégral de la publication scolaire au format pdf et consulter son résumé en ligne lorsque ces informations sont inclues dans les métadonnées.

Articles de revues sur le sujet "Cryo-CLEM"

1

Metskas, Lauren Ann, et John A. G. Briggs. « Fluorescence-Based Detection of Membrane Fusion State on a Cryo-EM Grid using Correlated Cryo-Fluorescence and Cryo-Electron Microscopy ». Microscopy and Microanalysis 25, no 4 (14 mai 2019) : 942–49. http://dx.doi.org/10.1017/s1431927619000606.

Texte intégral
Résumé :
AbstractCorrelated light and electron microscopy (CLEM) has become a popular technique for combining the protein-specific labeling of fluorescence with electron microscopy, both at room and cryogenic temperatures. Fluorescence applications at cryo-temperatures have typically been limited to localization of tagged protein oligomers due to known issues of extended triplet state duration, spectral shifts, and reduced photon capture through cryo-CLEM objectives. Here, we consider fluorophore characteristics and behaviors that could enable more extended applications. We describe how dialkylcarbocanine DiD, and its autoquenching by resonant energy transfer (RET), can be used to distinguish the fusion state of a lipid bilayer at cryo-temperatures. By adapting an established fusion assay to work under cryo-CLEM conditions, we identified areas of fusion between influenza virus-like particles and fluorescently labeled lipid vesicles on a cryo-EM grid. This result demonstrates that cryo-CLEM can be used to localize functions in addition to tagged proteins, and that fluorescence autoquenching by RET can be incorporated successfully into cryo-CLEM approaches. In the case of membrane fusion applications, this method provides both an orthogonal confirmation of functional state independent of the morphological description from cryo-EM and a way to bridge room-temperature kinetic assays and the cryo-EM images.
Styles APA, Harvard, Vancouver, ISO, etc.
2

Moser, Felipe, Vojtěch Pražák, Valerie Mordhorst, Débora M. Andrade, Lindsay A. Baker, Christoph Hagen, Kay Grünewald et Rainer Kaufmann. « Cryo-SOFI enabling low-dose super-resolution correlative light and electron cryo-microscopy ». Proceedings of the National Academy of Sciences 116, no 11 (26 février 2019) : 4804–9. http://dx.doi.org/10.1073/pnas.1810690116.

Texte intégral
Résumé :
Correlative light and electron cryo-microscopy (cryo-CLEM) combines information from the specific labeling of fluorescence cryo-microscopy (cryo-FM) with the high resolution in environmental context of electron cryo-microscopy (cryo-EM). Exploiting super-resolution methods for cryo-FM is advantageous, as it enables the identification of rare events within the environmental background of cryo-EM at a sensitivity and resolution beyond that of conventional methods. However, due to the need for relatively high laser intensities, current super-resolution cryo-CLEM methods require cryo-protectants or support films which can severely reduce image quality in cryo-EM and are not compatible with many samples, such as mammalian cells. Here, we introduce cryogenic super-resolution optical fluctuation imaging (cryo-SOFI), a low-dose super-resolution imaging scheme based on the SOFI principle. As cryo-SOFI does not require special sample preparation, it is fully compatible with conventional cryo-EM specimens, and importantly, it does not affect the quality of cryo-EM imaging. By applying cryo-SOFI to a variety of biological application examples, we demonstrate resolutions up to ∼135 nm, an improvement of up to three times compared with conventional cryo-FM, while maintaining the specimen in a vitrified state for subsequent cryo-EM. Cryo-SOFI presents a general solution to the problem of specimen devitrification in super-resolution cryo-CLEM. It does not require a complex optical setup and can easily be implemented in any existing cryo-FM system.
Styles APA, Harvard, Vancouver, ISO, etc.
3

Li, Shuoguo, Gang Ji, Xiaojun Huang, Lei Sun, Jianguo Zhang, Wei Xu et Fei Sun. « A New Solution of Non-integrated Correlative Light and Electron Microscopy Based on High-vacuum Optical Platform ». Microscopy and Microanalysis 22, S3 (juillet 2016) : 248–49. http://dx.doi.org/10.1017/s1431927616002099.

Texte intégral
Résumé :
Abstract Correlative light and electron microscopy (CLEM) offers a means of guiding the search for the unique or rare events by fluorescence microscopy (FM) and allows electron microscopy (EM) to zoom in on them for subsequent EM examination in three-dimensions (3D) and with nanometer-scale resolution. FM visualizes the localization of specific antigens by using fluorescent tags or proteins in a large field-of-view to study their cellular function, whereas EM provides the high level of resolution for complex structures. And cryo CLEM combines the advantages of maintaining structural preservation in a near-native state throughout the entire imaging process and by avoiding potentially harmful pre-treatments, such as chemical fixation, dehydration and staining with heavy metals. Besides for frozen-hydrated biological samples, CLEM combines the advantages of a close-to-life preservation of biological materials by keeping them embedded in vitreous ice throughout the entire imaging process and the frozen-hydrated condition is very suitable to maintain fluorescent signals. In recent years, many new instruments and software which intended to optimize the workflow and to obtain better experimental results of CLEM have been presented or even commoditized. While, the specimen damage during transfer from FM to EM and the resolution of CLEM were still need to be improved. Here we set up a High-vacuum Optical Platform to develop CLEM imaging technology (HOPE), which was designed to realize high-vacuum optical ( fluorescent) imaging for cryo-sample on EM cryo-holder (e.g. Gatan 626). A non-integrated high-vacuum cryo-optical stage, which adapted to the EM cryo holder, was fixed on epi-fluorescence microscope (or super-resolution microscope) to obtain fluorescent images. And then the EM cryo holder would be transferred to EM for collection of EM data. This protocol was aimed to minimize the specimen damage during transfer from FM to EM and it was versatile to expend to different types of light microscopy or electron microscopy. Our HOPE had already passed correlative imaging test, and the results showed that it was convenient and effective.
Styles APA, Harvard, Vancouver, ISO, etc.
4

Hampton, Cheri M. « Practical Strategies for cryo-CLEM Experiments ». Microscopy and Microanalysis 23, S1 (juillet 2017) : 1400–1401. http://dx.doi.org/10.1017/s1431927617007668.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
5

Thomas, Connon I., Nicolai T. Urban, Ye Sun, Lesley A. Colgan, Xun Tu, Ryohei Yasuda et Naomi Kamasawa. « Cryo-Confocal Imaging for CLEM Mapping in Brain Tissues ». Microscopy Today 29, no 5 (septembre 2021) : 34–39. http://dx.doi.org/10.1017/s1551929521001073.

Texte intégral
Résumé :
Abstract:In correlative light and electron microscopy (CLEM) workflows, identifying the same sub-cellular features in tissue by both light (LM) and electron microscopy (EM) remains a challenge. Furthermore, use of cryo-fixation for EM is desirable to capture rapid biological phenomena. Here, we describe a workflow that incorporates cryo-confocal laser scanning microscopy into the CLEM process, mapping cells in brain slices to re-image them with serial section scanning electron microscopy (ssSEM) array tomography. The addition of Airyscan detection increased the signal-to-noise ratio (SNR), allowing individual spines in thick frozen tissue to be visualized at a sufficient spatial resolution, providing a new tool for a CLEM approach to capture biological dynamics.
Styles APA, Harvard, Vancouver, ISO, etc.
6

Kamp, Arnold, Martijn van Nugteren, Hildo Vader, Michael Schwertner, Duncan Stacey, Roman Koning et Bram Koster. « Automated Cryo-plunging Robot to Prepare Samples for Single Particle Analysis (SPA), Cryo-EM, Cryo-ET, Cryo-fluorescence and Cryo-CLEM ». Microscopy and Microanalysis 26, S2 (30 juillet 2020) : 2732–33. http://dx.doi.org/10.1017/s1431927620022606.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
7

Paraan, Reza, Victoria Hewitt, Yusuke Hirabayashi, Franck Polleux, Clint Potter et Bridget Carragher. « Characterization of ER-mitochondria contact sites using cryo-CLEM ». Microscopy and Microanalysis 27, S1 (30 juillet 2021) : 1712–13. http://dx.doi.org/10.1017/s1431927621006255.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
8

Schwertner, Michael, et Duncan Stacey. « Cryo-Correlative Light and Electron Microscopy (Cryo-CLEM) : Specimen Workflow Paths and Recent Instrument Developments ». Microscopy and Microanalysis 21, S3 (août 2015) : 1565–66. http://dx.doi.org/10.1017/s1431927615008600.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
9

Sexton, Danielle L., Steffen Burgold, Andreas Schertel et Elitza I. Tocheva. « Super-resolution confocal cryo-CLEM with cryo-FIB milling for in situ imaging of Deinococcus radiodurans ». Current Research in Structural Biology 4 (2022) : 1–9. http://dx.doi.org/10.1016/j.crstbi.2021.12.001.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
10

Shahmoradian, Sarah, Jim Monistrol, Hung Tri Tran, Valerie Perez, Jenny Jiou, Jaime Vaquer-Alicea et Marc Diamond. « Abstract 2445 Elucidating Tau Fibril Formation using Correlative Cryo-CLEM in situ ». Journal of Biological Chemistry 300, no 3 (mars 2024) : 107098. http://dx.doi.org/10.1016/j.jbc.2024.107098.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
Plus de sources

Thèses sur le sujet "Cryo-CLEM"

1

Patra, Gurudatt. « Structure of mitotic chromosome and the role of condensin protein in the structural organization of chromosomes ». Electronic Thesis or Diss., Strasbourg, 2024. http://www.theses.fr/2024STRAJ020.

Texte intégral
Résumé :
Au cours de la mitose, la chromatine interphasique subit une compaction massive en structures en forme de bâtonnets. Les condensines sont des complexes protéiques dont on sait qu'ils jouent un rôle majeur dans l'organisation des chromosomes mitotiques. Les eucaryotes possèdent deux complexes de condensines conservés, à savoir les condensines 1 et 2. Des études in vitro sur des modèles d'ADN nus montrent que les condensines ont une activité d'extrusion de boucles dans l'organisation des chromosomes. Cependant, il reste encore beaucoup à explorer en ce qui concerne l'étude de la fonction des condensines dans l'environnement encombré de la chromatine. Nous avons utilisé la technologie halo tag où le domaine SMC2 des condensines est marqué par fluorescence à l'aide d'un ligand halo TMR. Cette approche nous aide à localiser les régions riches en condensines dans les chromosomes mitotiques partiellement décondensés en utilisant la cryo-microscopie en lumière à l'intérieur des chromosomes vitrifiés pour les études de cryo-tomographie électronique. Nos tomographies montrent les complexes de condensine dans l'environnement chromatinien. Cela ouvre une fenêtre sur l'étude de l'activité de liaison à l'ADN de la condensine, l'oligomérisation ou le regroupement de la condensine et son interaction avec d'autres composants non histoniques des chromosomes mitotiques
During mitosis, the interphase chromatin undergoes a massive round of compaction into rod-shaped structures. Condensins are protein complexes that have been known to play a major role in mitotic chromosome organization. Eukaryotes have two conserved condensin complexes, namely condensin 1 and 2. In vitro studies on naked DNA templates show evidence for loop extrusion activity of condensins in chromosome organization. However, there is still a lot to explore regarding the study of condensin function inside the crowded chromatin environment. We have used halo tag technology where the SMC2 domain of condensins is tagged to fluorescently label using a halo TMR ligand. This approach helps us to locate condensin-rich regions in partially decondensed mitotic chromosomes using cryo-light microscopy inside the vitrified chromosomes for cryo-electron tomography studies. Our tomograms show condensin complexes inside the chromatin environment. This opens up a window into the study of DNA binding activity of condensin, the oligomerization or clustering of condensin and its interaction with other non-histone components of mitotic chromosomes
Styles APA, Harvard, Vancouver, ISO, etc.
2

Nocera, Giovanni Marco. « Microfluidic cryofixation for time-correlated live-imaging cryo-fluorescence microscopy and electron microscopy of Caenorhabditis elegans ». Doctoral thesis, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E4EA-8.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.

Chapitres de livres sur le sujet "Cryo-CLEM"

1

Zanetti-Domingues, Laura C., Michael Hirsch, Lin Wang, Tara A. Eastwood, Karen Baker, Daniel P. Mulvihill, Sheena Radford, Jim Horne, Paul White et Benji Bateman. « Toward quantitative super-resolution methods for cryo-CLEM ». Dans Methods in Cell Biology. Elsevier, 2024. http://dx.doi.org/10.1016/bs.mcb.2024.02.028.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.

Actes de conférences sur le sujet "Cryo-CLEM"

1

Smeets, Marit. « METEOR : an integrated top down cryo-CLEM imaging system ». Dans Microscience Microscopy Congress 2021 incorporating EMAG 2021. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.mmc2021.59.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
2

Schwertner, Michael. « Automated cryo-plunger for the preparation of vitrified cryo-samples for Single Particle Analysis (SPA), cryo-EM, cryo-ET and cryo-CLEM, based on a novel procedure replacing conventional blotting ». Dans European Microscopy Congress 2020. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.emc2020.1112.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
3

Schilling, Nicolas. « A cryo-preparation approach for immuno-fluorescence labelling of cell cultures and CLEM ». Dans European Microscopy Congress 2020. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.emc2020.867.

Texte intégral
Styles APA, Harvard, Vancouver, ISO, etc.
Vous pouvez également être intéressé par les bibliographies sur le sujet « Cryo-CLEM » pour d’autres types de sources :
Articles de revues
Nous offrons des réductions sur tous les plans premium pour les auteurs dont les œuvres sont incluses dans des sélections littéraires thématiques. Contactez-nous pour obtenir un code promo unique!

Vers la bibliographie