Littérature scientifique sur le sujet « Crude Polysaccharide »
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Articles de revues sur le sujet "Crude Polysaccharide"
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Chen, Yong-Feng, Wen-wen Jiang, Shi-qi Zhang, Jian-quan Kan et Yong Liang. « Antioxidant Activity and Characterization of One New Polysaccharide Obtained fromPerigord Truffle(Tuber huidongense) ». Evidence-Based Complementary and Alternative Medicine 2016 (2016) : 1–7. http://dx.doi.org/10.1155/2016/3537193.
Texte intégralRésumé :
As a medicinal and edible fungus parasitizing on the trees, Perigord Truffle (Tuber huidongense) is well known for its delicious taste, unique smell, and high medical value for healthcare. One new water-soluble nonstarch polysaccharide (PST-W with the yield of 0.41%) fromPerigord Truffle(Tuber huidongense) was purified and identified on structural characteristics for the first time. The characterizations of PST-W were studied on physicochemical properties, main components of monosaccharide(s), and molecular structure. The monosaccharide compositions of PST-W were studied and identified as glucan, only containing D-glucoses with the molecular structure of [→6)α-D-Glcp(1→6) α-D-Glcp (1→]nby methylation analysis and NMR. In the determination of total reducing capacity, the reducing abilities of polysaccharide extracts could be listed as vitamin C > PST-W > crude polysaccharides-3 > crude polysaccharides-2 > crude polysaccharides-1. All of PST-W, crude polysaccharides-2, and crude polysaccharides-3 were relatively good scavenger for 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl radicals with IC50of 2.81, 4.17, and 3.44 mg/mL, respectively. However,O2-∙clearing abilities of PST-W and crude polysaccharides were obviously weaker. The activities of total crude extract were the worst, indicating that the impurities might negatively affect the antioxidant activity. Thus, the separation and purification of polysaccharides were significant to increase the antioxidant activity in some degree.
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Olech, Marta, Natalia Nowacka-Jechalke, Maciej Masłyk, Aleksandra Martyna, Wioleta Pietrzak, Konrad Kubiński, Daniel Załuski et Renata Nowak. « Polysaccharide-Rich Fractions from Rosa rugosa Thunb.—Composition and Chemopreventive Potential ». Molecules 24, no 7 (6 avril 2019) : 1354. http://dx.doi.org/10.3390/molecules24071354.
Texte intégralRésumé :
The huge health-beneficial potential of polysaccharides encourages the search for novel sources and applications of these compounds. One poorly explored source of polysaccharides is the rose. The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb. were studied for their composition and the influence on various cellular processes involved in the development of cancer and other civilization diseases. The study revealed the presence of water-soluble and -insoluble polysaccharides (including β-glucans) and protein-polysaccharide conjugates in rose organs. Rose hips were found to be the most abundant source of polysaccharides. Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays. Therefore, rose crude polysaccharides are very promising and can potentially be used as natural chemopreventive agents.
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Wang, Lin, Runyong Fan, Funeng Geng, Yongxiang Gao et Qinwan Huang. « Protective effect of crude polysaccharide from Pao-TianXiong derived from monkshood, against chronic renal failure in mice ». Tropical Journal of Pharmaceutical Research 18, no 6 (27 mai 2021) : 1235–43. http://dx.doi.org/10.4314/tjpr.v18i6.13.
Texte intégralRésumé :
Purpose: To investigate the effect of crude polysaccharide isolated from pao-tian-xiong on chronic renal failure in mice, and its monosaccharide composition.
Methods: Male Kunming mice were orally treated with adenine (211.5 mg/kg/day) for 7 days, followed by either crude polysaccharides (125, 250 or 500 mg/kg), or positive drug solution (jinguishenqi pill, 2000 mg/kg) for another 7 days (each group had 15 mice). Mice in normal and negative control groups were given saline. Mental and physical states, blood urine nitrogen (BUN) and serum creatinine (SCr), kidney morphological changes and organ indices were determined. Histopathological examination of spleen and kidney tissues was also performed. The monosaccharide composition of crude polysaccharide was determined by high-performance liquid chromatography (HPLC) and gas chromatography and mass spectrometry (GC-MS).
Results: Compared with negative control group, serum BUN (6.71 mmol/L vs. 8.61 mmol/L) and Cr (107.74 vs. 113.39 μmol/L) were significantly decreased by the crude polysaccharide isolate (p < 0.05), whereas epididymis index (0.2556 vs. 0.2135 %) and seminal vesicle index (0.5547 vs. 0.3945 %) were increased (p < 0.05). Histopathological examination showed that injuries to kidney, spleen, testis and epididymis decreased significantly. The crude polysaccharides contained mainly glucose, rhamnose, arabinose, galactose, mannose, galacturonic acid, glucuronic acid and xylose, and their contents ranged from 0.7 to 65 %.
Conclusion: These results suggest that the crude polysaccharides of Pao-tian-xiong ameliorates CRF symptoms in mice, thereby providing experimental evidence in support of its use as an anti-CRF drug.
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Al-Wraikat, Majida, Yun Liu, Limei Wu, Zeshan Ali et Jianke Li. « Structural Characterization of Degraded Lycium barbarum L. Leaves’ Polysaccharide Using Ascorbic Acid and Hydrogen Peroxide ». Polymers 14, no 7 (30 mars 2022) : 1404. http://dx.doi.org/10.3390/polym14071404.
Texte intégralRésumé :
Plant-derived polysaccharide’s conformation and chain structure play a key role in their various biological activities. Lycium barbarum L. leaves’ polysaccharide is well renowned for its health functions. However, its functional bioactivities are greatly hindered by its compact globular structure and high molecular weight. To overcome such issue and to improve the functional bioactivities of the polysaccharides, degradation is usually used to modify the polysaccharides conformation. In this study, the ethanol extract containing crude Lycium barbarum L. leaves’ polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves’ polysaccharide. To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography–mass spectrometry (GC–MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM). Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition. However, the molecular weight (Mw) had decreased significantly from 223.5 kDa to 64.3 kDa after degradation, indicating significant changes in the polysaccharides molecular structure caused by degradation.
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Zhang, Qiang, Lianxiang Lu, Yanfei Zheng, Chengrong Qin, Yuexin Chen et Zhongjie Zhou. « Isolation, Purification, and Antioxidant Activities of Polysaccharides from Choerospondias axillaris Leaves ». Molecules 27, no 24 (14 décembre 2022) : 8881. http://dx.doi.org/10.3390/molecules27248881.
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The extraction, characterization and antioxidant activity of polysaccharides from Choerospondias axillaris leaves were investigated in the present study. Two purified polysaccharide fractions, CALP-1 and CALP-2, were isolated from crude Choerospondias axillaris leaf polysaccharides (CALP) by DEAE-52 cellulose chromatography and Sephadex G-100 column chromatography. The characteristics of CAL-1 and CALP-2 were determined by using High-performance Gel Permeation Chromatography (HPGPC), High-Performance Anion-Exchange Chromatography, HPAEC (HPAEC-PAD) and Fourier transform infrared spectroscopy (FTIR). CALP-1 with molecular weight of 11.20 KDa was comprised of Rhamnose, Arabinose, Galactose, Glucose, Xylose, Mannose and galacturonic acid in a molar ratio of 5.16:2.31:5.50:27.18:1.00:0.76:1.07. CAL-2 with molecular weight of 8.03 KDa consisted of Rhamnose, Arabinose, Galactose, Glucose, and galacturonic acid at a ratio of 1.38:3.63:18.84:8.28:1.45. FTIR revealed that CALP-1 and CALP-2 were acidic polysaccharides. The antioxidant activity of crude CALP, CALP-1 and CALP-2 was evaluated in vitro. The fraction CALP-2 was demonstrated to be of polysaccharide nature containing a large percentage of Galactose but no Xylose and Mannose. The antioxidant activity assays showed that CALP-1 and CALP-2 exhibited antioxidant and scavenging activities on hydroxyl and DPPH radicals in vitro. Compared with pure polysaccharide, crude CALP exhibited stronger anti-oxidant activities. These results will provide a better understanding of Choerospondias axillaris leaf polysaccharide and promote the potential applications of Choerospondias axillaris leaf polysaccharide in the pharmacological field and as a natural antioxidant.
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Li, Tingting, Yan Yang, Linjun Chen et Wanling Yang. « Effects of Grinding Methods on Physicochemical Properties of Crude Polysaccharides from Phellinus baumii ». E3S Web of Conferences 78 (2019) : 02004. http://dx.doi.org/10.1051/e3sconf/20197802004.
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The physicochemical properties of Phellinus baumii polysaccharides extracted from the fruiting bodies by different grinding methods were studied in this paper. Compared with ordinary grinding methods, superfine grinding technology exerts significantly higher yields of crude polysaccharides, polysaccharide contents and β-Glucan contents of six samples. HPSEC-MALLS- RI analysis shows the molecular weight distribution of P.baummii crude polysaccharides, which indicates superfine grinding technique generates higher molecular weight fractions and larger polydispersities. The 6 kinds of crude polysaccharides have obvious effects on NO generation of RAW264.7 cells, which indicated that crude polysaccharides from P.baumii may have some immune-enhancing effects. Meanwhile, CW30 and FP30 show the best immune enhancing effects. However, CW50 and CW70 exert weaker immune-enhancing effects compared with FP50 and FP70.
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Cao, Ling, Mi Yu, Chonghui Wang, Yunhui Bao, Minghui Zhang, Ping He, Yan Zhang et al. « Cellulase-Assisted Extraction, Characterization, and Bioactivity against Rheumatoid Arthritis of Astragalus Polysaccharides ». International Journal of Polymer Science 2019 (7 février 2019) : 1–9. http://dx.doi.org/10.1155/2019/8514247.
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This study investigated the effect of cellulase on the isolation of crude Astragalus polysaccharide (APS), analyzed the monosaccharide component of deproteinized APS, detected the molecular weights of purified APS, and examined the biological activities and the preliminary mechanism against rheumatoid arthritis (RA). Compared with water extraction method, cellulase-assisted extraction increased the yield of crude APS to 154% and polysaccharide contents to 121%. Crude APS was then purified by ethanol precipitation, Sevag deproteinization, and high-performance liquid chromatography (HPLC) analysis; monosaccharide contents of APS were different after cellulase-assisted method, especially galacturonic acid content which significantly increased. DEAE-52 cellulose column chromatography isolated three polysaccharide fractions, including a neutral polysaccharide (APS-water) and two acidic polysaccharides (APS-NaCl1 and APS-NaCl2). Using high-performance gel permeation chromatography (HPGPC), the molecular weights of APS-water, APS-NaCl1, and APS-NaCl2 were identified as 67.7 kDa, 234.1 kDa, and 189.4 kDa, respectively. Then their therapeutic effects and possible mechanism against RA were explored using type II collagen-induced arthritis (CIA) rat model. APS could significantly reduce paw swelling, serum concentration of IL-1β and TNF-α, and the expression levels of NF-κB-p65 and IκBα in synovial membranes in CIA rats. Our study indicated that cellulase significantly increases the yield and polysaccharide contents of crude APS, improves the product quality, and preserves the biological features against RA in CIA rats.
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Li, Yinfeng, Fei Chen et Xiaozhu Liu. « Effects of Rosa roxburghii Tratt Must on the Growth, Nutrient Composition, and Antioxidant Activity of Pleurotus ostreatus Mycelia ». Molecules 27, no 11 (2 juin 2022) : 3585. http://dx.doi.org/10.3390/molecules27113585.
Texte intégralRésumé :
Rosa roxburghii Tratt, a Rosaceae plant endemic to China, produces fruit with high nutritional and medicinal value. The effects of R. roxburghii must on the growth, nutrient composition, and antioxidant activity of Pleurotus ostreatus mycelia was investigated. We measured the mycelial growth rate, proximate composition, amino acid and crude polysaccharide content, and the antioxidant activity of the crude polysaccharides of P. ostreatus mycelia cultivated under different concentrations of R. roxburghii must (2%, 4%, and 8%, v/v). Low concentrations of R. roxburghii must (2% and 4%) promoted mycelial growth, while a high concentration (8%) inhibited mycelial growth. Low concentrations of R. roxburghii must had no significant effects on the soluble substances, fat, ash, and crude fiber in P. ostreatus mycelia, but significantly increased the crude protein and total amino acid contents (p < 0.05). The addition of R. roxburghii must at low concentrations significantly increased the crude polysaccharide content in mycelia (p < 0.05) but had no impact on the scavenging of hydroxyl radicals and 2,2-diphenyl-1-picrylhydrazyl (DPPH). Therefore, R. roxburghii must at low concentration can be used as a substrate for P. ostreatus cultivation to increase the protein and polysaccharide contents in mycelia.
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Nakamura, Michiko, Sayaka Miura, Akiko Takagaki et Fumio Nanjo. « Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide ». International Journal of Food Sciences and Nutrition 68, no 3 (21 septembre 2016) : 321–30. http://dx.doi.org/10.1080/09637486.2016.1232376.
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Souissi, Nabil, Soumaya Boughriba, Ola Abdelhedi, Marwa Hamdi, Mourad Jridi, Suming Li et Moncef Nasri. « Extraction, structural characterization, and thermal and biomedical properties of sulfated polysaccharides from razor clam Solen marginatus ». RSC Advances 9, no 20 (2019) : 11538–51. http://dx.doi.org/10.1039/c9ra00959k.
Texte intégralRésumé :
In this study, some biological activities of sulfated polysaccharides extracted from Solen marginatus flesh were investigated via physicochemical characterization of the crude polysaccharide SM-CP and its deproteinized fraction SM-DP.
Thèses sur le sujet "Crude Polysaccharide"
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Sarip, Harun. « Optimization of DIC assisted hydrolytic conversion of polysaccharides (starch and cellulose) ». Phd thesis, Université de La Rochelle, 2012. http://tel.archives-ouvertes.fr/tel-00986304.
Texte intégralRésumé :
Present state of art related to biomass conversion technology so far was found to concentrate on an enzymatic process, coupled with thermal pretreatment on biomass rich in cellulose. Biomass that rich in crude starch is also important in terms of strategic and economic point of view. The main objective of this study is to adopt a new strategy for a single step conversion of a crude starch material into oligosaccharide and glucose utilizing DIC technology. In contrast to existing thermal based pretreatment, DIC technology involves two vacuum cycles; first vacuum cycle was to increase steam accessibility on biomass and to reduce generation of steam condensate thus avoid losing of monosaccharide and hemicelluloses, while second vacuum cycle was to reduce potential thermal degradation of glucose. Distributions of products formed were found to be closely associated with severity of treatment on crude starch material. At lower DIC severity, pretreatment favors the formations of high oligosaccharide composition with small fraction of glucose; while at high DIC severity, pretreatment favors formation of high glucose as a major end product. During an exploratory study to establish the relevant reaction factors; vacuum cycle and moisture content were the two main factors influencing the conversion of crude starch into glucose.DIC starch conversion into glucose was found to be moisture dependent. Both factors were combined together to optimize the other three factors: pressure/temperature, treatment times, and acid concentration. High DIC severity treatment alone could convert nearly 50% of crude starch into glucose. During DIC optimization, an experimental design was developed and tested with DIC pretreatment in order to obtain a second order polynomial mathematical model that was then applied for response surface methodology (RSM). The interaction nature of above factors was examined and was found they depend on DIC treatment severity. Two experimental designs with low and high DIC severity were developed; Low DIC severity (acid: 0.01-0.05 molar, time: 0.5-3.0 min) and High DIC severity (acid: 0.05-0.20 molar, time: 3.0-10.0 min) with similar temperature range (144-165oC) were used. Data mining operation was done on RSM model to develop a kinetic model at both treatment severities. Kinetic data, including rate constant and activation energy were calculated from kinetic models of both severities to compare with actual dilute acidhydrolysis kinetic studies on two DIC treated samples. It was found that activation energy (Ea)for glucose generation at High DIC severity (Ea: 59.44 kJ/mol) was lower than at optimum dilute acid hydrolysis (Ea: 91.30 kJ/mol); while for glucose degradation, Ea was higher with High DIC severity (Ea: 144.12 kJ/mol) if compared to dilute acid hydrolysis (Ea: 45.14 kJ/mol).This indicates that glucose generation with DIC requires less energy while its degradation needs high energy. This combination was required to maximize glucose generation and minimize glucose degradation. Further studies with non-isothermal state during DIC and dilute acid hydrolysis support this finding. In normal polysaccharide conversion to low molecular weight (LMW) oligosaccharides and glucose procedures; two process steps were involved, namely the first process involved thermal pretreatment followed by a second process with dilute acid hydrolysis. In the present work, attempt was made to exclude dilute acid hydrolysis stage in order to establish that DIC process alone is sufficient for total polysaccharides conversion into LMW mainly glucose fraction. Information gathered from quantitative and statistical analysis on (i) exploratory studies, (ii) kinetic models from RSM of DIC process and (iii) kinetic data based on experimental works during dilute acid hydrolysis study; support the assumption that DIC treatment alone is sufficient for the total conversion required.
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Chu, Yi-Chia, et 朱羿嘉. « Antioxidant, Anti-inflammatory Activity and Polysaccharide Compositions of Crude Extracts of Corchorus Capsularis (L) ». Thesis, 2013. http://ndltd.ncl.edu.tw/handle/99305087623026244910.
Texte intégralRésumé :
碩士靜宜大學
食品營養學系
102
Corchorus capsularis , also known as jute or jews mallow, is atropical and subtropical crop. Not only providing high fiber for rope making, the leaves of jute is rich in mucilage that is good for soup traditionally, one of famous local snack. The purposes of this study are to investigate the antioxidant and anti-inflammatory activity of jute’s extracts, andto compare the physicochemical properties of crude polysaccharides among the extracts. The antioxidant and anti-inflammatory activity of the extracts of jute from different ratios of ethanol were also determined. Moreover, three extracted methods, including cold water (CCP), hot water (CHP) and ultrasonic (CUP) extraction, were usedto determine the yield and chemical compositions of crude polysaccharides. The monosaccharide compositions, functional groups, molecular weight distribution and viscosity of polysaccharides were also analyzed in this study. The results indicated that theprimaryproximate compositions of the leaves of juteare carbohydrate and protein, up to 25.13% in protein. The potassium is the most abundant mineral, up to 623.2 mg %, following by calcium, magnesium, sodium and iron. No significant difference was observed in the yield of crude polysaccharide among three extracts. Antioxidant activity assay revealed that the Ce50 extracts obtained the best ferrous ion chelating ability. The chelating effect reached 91% as the concentration of extracted was at 3 mg/ml. The best ABTS radicals and DPPH free radical scavenging capacity of extracts were obtained in Ce95 method up to 98% and 89 %, respectively, in both 50 μg/mL and 5 mg/mL. The higher concentration of crude extracts of jute the lower cell viability were obtained on the growth of Raw264.7 cells. As the concentration of extracts increased to 500μM/mL, cell viability of Raw264.7 decreased to 20% on the Ce50 and Ce95 extracts except Cw extracts. The inhibition of NO production increases of concentration of jute’s extracts up to 50μM/mL. The highest inhibition with the increased was found in Ce95 extracts, up to 68.3%. There finding indicated that Ce95 extracts may have potential application in the antioxidant and anti-inflammatory activity of health foods development. Comparison of cold water, hot water, and ultrasonic extraction in the yield of extracts, the highest yield of extracts was found in ultrasonic extraction, up to 25%, which more than twice of hot water and cold water in the yield. The same monosaccharide compositions in three crude polysaccharides demonstrated extracted method did not influence the compositions of monosaccharide. Glucose is the primary monosaccharide, following by galactose, arabinose, rhamnose and xylose. Highuronic acid content was identified in crude polysaccharides of jute resulted in an acidic polysaccharides. The CHP extracted method obtained the higher total carbohydrate and uronic acid in the crude polysaccharides, 42% and 61 mg/g, respectively. The Fourier transform infrared spectra revealed and indicated the general characteristicabsorption peaks of three polysaccharides were similar. Non-Newtonian fluidbehavior was observed in three polysaccharides solutions which indicated the viscosity increasing with the increases of concentration of polysaccharides, but decreasing with the increases of temperature. The highest viscosity was located at CHP extracted solution.
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WANG, TING-YIN, et 王亭尹. « To Study the Growth Inhibitory Effect of Crude Polysaccharide from Inonotus sp. in Human Monocytic Leukemia Cells ». Thesis, 2016. http://ndltd.ncl.edu.tw/handle/q6zkfm.
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Hsu, Yo-Ru, et 許攸如. « Preparation and Biofunctional Properties of Water-Soluble Chitosan-Laminaria japonica Crude Polysaccharide Nanoparticles and its Application in Cosmetics ». Thesis, 2008. http://ndltd.ncl.edu.tw/handle/46630483593934464983.
Texte intégralRésumé :
碩士國立臺灣海洋大學
食品科學系
96
Laminaria sp. is the largest throughput with important economical among cultivated macroalgaes in the world, and Taiwan is the main import country. The aims of this study were to prepare a new nanoparticle (Laminariasphere) system via ionic gelation of Laminaria japonica crude polysaccharide hydrolysate and low molecular weight chitosan (LMCS), and to evaluate effect of LJCPH and LMCS-LJCPH nanoparticles on cell viability and collagen secretion capability on cultured human normal skin fibroblast (CCD-966SK) and to finding out the optimal concentration for skincare products. An anti-aging skincare containing the LMCS-LJCPH nanoparticles was prepared and its characteristics including storage stability, safety and effecacy such as the skin elasticity, skin hydration, skin roughness and skin sensitive were measured. The results obtained were that both crude polysaccharide (LJCP) and crude polysaccharide hydrolysate (LJCPH) from Laminaria japonica showed anti-oxidation capabilities. The functional compositions result shows LJCP were lower in total sugar, reducing sugar, polyphenol and flavonid than that of LJCPH, but has a similar sulfate content. Anti-oxidation activities data showed that anti-oxidation capabilies of LJCPH were better than that of LJCP and anti-oxidation capability in general increased by increasing the concentration of the sample. Low molecular weight chitosan (LMCS) were prepared by hydrogen peroxide (H2O2) degradation. The LMCS solution were prepared by varying the volume ratios, stirring time, stirring speeds to prepare nanoparticle of mean diameter of 100-200 nm, zeta potential at＋28 ± 3 (mV) and spherical shape observed by SEM. The ionic interations occurred between LMCS and LJCPH as elucidated by FT-IR spectra. There are no obvious change during storage at pH 5.5-6.6 solutions and 4, 25�aC for 28 day, the results shows that the nanoparticles is stable during storage period. LJCPH and LMCS-LJCPH nanoparticles at 62.5-10000 μg/ml have higher cell viability than control. The collagen secretion capability of the cultured human normal skin fibroblast increased when the concentration of LJCPH and LMCS-LJCPH nanoparticles at 250 μg/ml. The enhancement were more pronounced of LMCS-LJCPH nanoparticles on collagen secretion capability than that of LJCPH. Proliferation effect of LMCS-LJCPH nanoparticles on Keratinocyte (HaCaT) cell viability is higher than that LJCPH. pronoumced The cosmetic products containing either with or without LMCS-LJCPH nanoparticles were stable after sonication (135 W, 42KHz, 30 min), after centrifugation (3000 rpm, 30 min), and after more than four storage months at high temperature (50 �aC). The safety test resulted in no erythema based on the Daize score test. Skin eleasticity (R2) for those whom have applied the cosmetic product containing LMCS-LJCPH nanoparticles increased over time during six weeks test period. Skin roughness decreased over time during six weeks test period and did show a significant difference for skin scaliness, skin wrinkles index, skin smoothnes and skin roughness.
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Lu, Jia Jun, et 呂嘉均. « Extraction of crude polysaccharide extracts from Agricus brasiliensis and its effect on cellular viability in human hepatoma cells ». Thesis, 2009. http://ndltd.ncl.edu.tw/handle/85208563692704013193.
Texte intégralRésumé :
碩士亞洲大學
生物科技學系碩士班
97
Agaricus brasiliensis is a native mushroom of Brazil, South America. It is considered as an culinary-medical and functional food, a rare specie of mushroom rich in nutritious substance and living organism which enhance the immune system, antitumor, antioxidant,antivirus,and antimutagenic, abilities. Lately, scientific researchers have found that the polysaccharide of this mushroom is effective for the control of tumor cells. For this research, the fresh fruiting body of A. brasiliensis was refrigerated, dried, and ground into powder. With various temperatures of hot water or various times of Phosphate buffer saline combined with ultrasonic oscillation, 5.68 mg/ml of crude polysaccharides were extracted from 10g powders of Agaricus brasiliensis fruiting body by 100℃ hot wather, and 8.08 mg/ml polysaccharides by 15 min ultrasonic oscillation. The result also revealed that the quantity of polysaccharide contained in the cap of mushroom was 8.37mg/ml, which was higher than the 7.63mg/ml of polysaccharide contained in the stalk. The effects of crude polysaccharide on human liver cancer cell（HepG2､Hep3B）and normal liver cell（Chang liver cell）were studied using LDH assay and MTT assay to analyze the viability of liver cells. And using ALT assay and AST assay were used to analyze the metabolism of the liver cell function activity. The result showed that the Chang liver cell or liver cancer cell(HepG2, Hep3B) cultured with 0.32 mg/ml crude polysaccharide for 48 hours could increase Chang liver cells growth and reduce to LDH activity.On the other hand, the liver cancer cells (Hep G2，Hep3B) growth was restrained, yet their LDH activity was increased: HepG2 to 40% and Hep3B to 45%. The observation of microscope showed that cells of HepG2 and Hep3B were lysed after treating with A. brasiliensis crude polysaccharides. Some of cells appeared to be shrunk, empty and became smaller. The best concentration of crude polysaccharide among four treatments was 0.32 mg/ml. After 48 hour incubation, the AST and ALT activities of normal Chang liver cell were increased 5% and 60% indiviedually, where the AST and ALT of HepG2 were decreased 38% and 45%, and those of Hep3B were decreased 35% and 55% respectively.
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KHATIB, MOHAMAD. « BIOACTIVE COMPOUNDS INTO EDIBLE SYRIAN PLANTS : POMEGRANATE AND CAPPER ». Doctoral thesis, 2015. http://hdl.handle.net/2158/1045230.
Texte intégralRésumé :
The research developed within this PhD thesis has been developed according to different specific objectives depending on the selected plant. Overall the choice of these two matrices, the fruit of pomegranate and the root of capper, is related to the importance of these plant in Syria.
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Huang, jianin, et 黃建霖. « Study of Physicochemical Properties on Crude Polysaccharides from Ulva lactuca ». Thesis, 2013. http://ndltd.ncl.edu.tw/handle/83716824306646465571.
Texte intégralRésumé :
碩士國立宜蘭大學
食品科學系碩士班
101
Ulva lactuca belongs to Chlorophyceae class, Ulvales order, Ulvaceae family, and Ulva genus. It is commonly seen in the northeastern coast of Taiwan, and peak product in February to April. In recent years, Deep-sea water has been applied on Ulva lactuca farming, which has some characteristics such as stable quality and easy processing. Ulva lactuca have known to possess an ingredient of sulfated polysaccharides that have been widely used as curative agents for anti-virus, anti-tumor, anti-oxidation, anti-coagulant, and control of normal cholesterol levels. However, the physicochemical properties is unclear. The purpose of this study was investigated the physicochemical properties of crude polysaccharides including hydration and rheological and gelation. The results showed that solubility were affected by the solution of temperature and pH, meantime had was the highest solubility at 65℃ and 80℃, the solubility is 97.08% and 96.32% respectively. When pH value is 2 to 5, the solubility is 95.80% to 96.18% respectively. The results showed that rheological properties were affected by the concentration and salts and pH value. When concentration increased to 1.5%, the viscoelasticity significantly rised. With increasing concentrations of calcium chloride. the viscoelasticity will be upgraded significantly. The results showed that gel strength affected by the addition of salts, with sodium chloride and calcium chloride concentration increases, the gel strength would be gradually increased and decreased the water loss rate. The gel structure due to different pH values affect gel strength, when pH10 the gel the strongest intensity, and as the pH decreased, the gel strength will gradually weaken, water loss increases.
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Huang, Chien-Feng, et 黃健峰. « Glucose Homeostatic Activities of Okra Crude Polysaccharides in Murine Insulin Resistance Model ». Thesis, 2015. http://ndltd.ncl.edu.tw/handle/87769295372961361570.
Texte intégralRésumé :
碩士東海大學
食品科學系
103
Okra (Abelmoschus esculents fruits) are rich in functional ingredients, including polysaccharides and flavonoids. Recent studies have demonstrated that okra may provide antioxidative, antibacterial, hepatorprotecetive, hypolipidemic and hypocholesterolemic effects. Previous studies have showed that insulin resistance of FL83B cells may be improved by okra flavonoid derivatives. Nevertheless, hypoglycemic mechanisms of okra polysaccharides have not yet been fully documented. In this study, glucose homeostatic activities of okra polysaccharides were investigated in an insulin reisistance murine model. The okra polysaccharide (70.65 g) was extracted from 500 g lyophilized powder after being soaked in hot water, precipitatied by 75% ethanol, and dried, giving 14.13% yield. The contents of total polysaccharides, total phenols, total proteins, of this crude extract were 91.30%, 7.48%, 2.31 %, respectively, and the MW was estimated to be 2.35×104 kDa. The mice were induced to be insulin resistant and hyperglycemic by high fructose, then were administered with okra’s polysaccharide. After sixteen weeks, okra feeding group showed significant improvement in oral glucose tolerance, serum hepatic function parameters, sTG, sTC, fructosamine, serum resistin, serum adiponectin, and hepatic TNF- production. Insulin signaling in hepatocytes was concordantly enhanced, as revealed by the upregulating the test group of protein or mRNA relative performance were enhanced, IR-β, PI3K, Akt, pAkt, AMPK, pAMPK and GLUT2,and the down regulation of PEPCK, GSK3β, FoxO1, in a dose-dependent manner. Moreover, okra’s polysaccharide seemed to ameliorate the pathological conditions in hepatic panlobular microvesicular and tissue arrangement as demonstrated by histological evaluation by H&E and oil-red-O stains. In conlusion, okra polysaccharide may improve glucose homeostasis by increasing hepatic isnulin sensitivity, glucose uptake, glycogen synthesis, while suppressing gluconeogenesis.
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Fan, Liling, et 范俐伶. « The study of crude polysaccharides of Sterculia lychnophora on the antioxidant and anti-inflammation activity ». Thesis, 2012. http://ndltd.ncl.edu.tw/handle/19728764022794958266.
Texte intégralRésumé :
碩士靜宜大學
食品營養學系
100
Sterculia lychnophora is one of the popular Chinese herb medicine which is used to relieve sore throat and cough traditionally. In this study, we extracted the polysaccharides from Sterculia lychnophora and investagated the chemical compositions, antioxidant activity and anti-inflammatory of the crude polysaccharides. The Ames test was used to evaluate the toxicity and mutagenic potential of the crude polysaccharides. Three extracted methods including water (SLW), 50% methanol (SLM) and 50% ethanol (SLE) extractions were used to extract polysaccharides from the seeds of Sterculia lychnophora. The results indicated that the yield of crude polysaccharides were similar among the three extracts, which were 19.95%, 21.54% and 19.09% for SLW, SLM and SLE respectively. The monosaccharide compositopns of crude among three crude polysaccharides were the same, including rhamnose, arabinose, galactose and glucose. The antioxidant activity of crude polysaccherides increased with the increase of concentration of SLW, SLM and SLE. The EC50 of DPPH radical scavenging, ABTS radical scavenging, and Ferrous metal ion chelating activity of crude polysaccharides ranged in 0.71-1.35 mg/mL, 1.88-46.13 g/mL, and 2.22-3.61 mg/mL, respectively. In vitro of anti-inflammatory of crude polysaccharides showed the added dose of 500 g/mL could promote the growth of RAW264.7 cells. As the dose of crude polysaccharides decreased to 62.5 g/mL, the best inhibitory of NO production were 50.75%, 56.17% and 39.51% for SLW, SLM and SLE, respectively. In the assessement of food safety, the Ames test was used to analyze the mutagenic potential of crude polysaccharides of Sterculia lychnophora. The results indicated the negative tests in toxicity and in mutagenic potential from S. tyhimurium TA were obtained according to the biological assay of Ames test.
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Shih, Yafang, et 施雅芳. « Chemical Proximate Analysis Antioxidant and Immunomodulation Activity of Crude Polysaccharides from Blanching Water of Flammulina Velutipes ». Thesis, 2011. http://ndltd.ncl.edu.tw/handle/54554524274647743595.
Texte intégralRésumé :
碩士大葉大學
生物產業科技學系
99
Flammulina velutipes is one of the common edible mushrooms on the market. The mainly edible mushrooms not only have many nutrient but also have high functional and medicinal values. Studies showed that the protein binding polysaccharide of F. velutipes has antioxidant, anti-tumor and immune regulation activities. In this study, investigation of the concentration of polysaccharide in the blaching water prodceeded, nevertheless, antioxidant activity, anti-tumor activity, cytotoxicity and immune activity of F. velutipes polysaccharide were also analyzed. In this study, crude polysaccharide of F. velutipes was extracted from blanching water by ethanolic extraction. It contains many nutrients including carbohydrate (45.95％), crude protein (17.52％), crude fat (1.47％), crude fiber (0.99％) and ash (21.36％). The antioxidant activity on DPPH scavenging ability of blanching water is 33％; crude polysaccharide showed a 46％; As for the ferrous ion chelating ability, blanching water showed a 44％ and crude polysaccharide has a ability of 98％. On reducing ability, blanching water has a 0.30, where crude polysaccharide showed a 1.73 at 1mg/mL. FTIR analysis of crude polysaccharide showed it contains β-1,3-glycosidic groups. In cellular toxicity test showed that crude polysaccharide can promote the HPBMC proliferation for the first 24 hours, however, the HPBMC of proliferation was inhibited after 72 hours incubation. The anti-tumor activity with human cutaneous malignant melanoma showed no inhibition of cellular proliferation after 24 hours incubation; crude polysaccharide from multiple numbers of blanching can increase the ablility of suppression of tumor cell proliferation after 72 hours incubation, however, the crude polysaccharide showed no inhibition on human oral cancer cell proliferation. Investigation of crude polysaccharide with HPBMC on the cytokine profile showed that after 24 hours incubation with 0.01～10 μg/mL concentration of crude polysaccharide, highest TNF-α secretion was found (1144 pg/mL), as for 72 hours incubation, TNF-α secretion was dropped to 312～645 pg/mL. Mononuclear cells can be effectively stimulated by crude polysaccharide, the effect is not lower else fungi. The results can improve value of Flammulina velutipes, and the crude polysaccharide can replace synthetic antioxidants. Delop value-added waste water recycling plants and environmental.
Chapitres de livres sur le sujet "Crude Polysaccharide"
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Monobe, Manami, Kaori Ema, Keiko Azuma et Mari Maeda-Yamamoto. « Enhancement of Phagocytic Activity by a Crude Polysaccharide from Tea (Camellia sinensis) Extract ». Dans Basic and Applied Aspects, 333–38. Dordrecht : Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3892-0_55.
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Yaniv, Oren, Sadanari Jindou, Felix Frolow, Raphael Lamed et Edward A. Bayer. « A Simple Method for Determining Specificity of Carbohydrate-Binding Modules for Purified and Crude Insoluble Polysaccharide Substrates ». Dans Biomass Conversion, 101–7. Totowa, NJ : Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-956-3_10.
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Zhang, Kaihua, Liming Zhang, Na Liu, Jianheng Song et Shuang Zhang. « Optimization of Crude Polysaccharides Extraction from Dioscorea esculenta by Response Surface Methodology ». Dans Lecture Notes in Electrical Engineering, 509–20. Berlin, Heidelberg : Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-45657-6_53.
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4
« Extraction technology of crude polysaccharide from ». Dans Advanced Engineering and Technology, 655–60. CRC Press, 2014. http://dx.doi.org/10.1201/b16699-101.
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5
Khatua, Somanjana, et Krishnendu Acharya. « Crude polysaccharides from two Russuloid myco-food potentiates murine macrophage by tuning TLR/NF-κB pathway ». Dans Biotechnology and Biological Sciences, 281–86. CRC Press, 2019. http://dx.doi.org/10.1201/9781003001614-47.
Texte intégralActes de conférences sur le sujet "Crude Polysaccharide"
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Fedonenko, Yu P., I. M. Ibrahim, E. N. Sigida, V. I. Safronova, M. S. Kokoulin, A. Yu Muratova et S. A. Konnova. « Bioremediation potential of a halophilic bacterium Chromohalobacter salexigens EG1QL3 : exopolysaccharide production, crude oil degradation, and heavy metal tolerance ». Dans 2nd International Scientific Conference "Plants and Microbes : the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.070.
Texte intégralRésumé :
Based on biochemical and phylogenetic analyses, isolated from a salt sample from Lake Qarun (Egypt) a halophilic strain EG1QL3 was identified as Chromohalobacter salexigens. The abilities of EG1QL3 to produce an extracellular polysaccharide, degrade oil, and resist to heavy metals were revealed.
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Lasker, S. E., B. Y. Lee et R. E. Madden. « LOW MOLECULAR WEIGHT HEPARINS s ORAL ABSORPTION IN MONKEYS ». Dans XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644855.
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An orally administered low molecular weight heparin-like derivative of the commercial polydisperse polysaccharide is desirable clinically. The dissociation of antithrombotic properties and the induction of bleeding as well as minimal effect on platelet function are characteristics of some low-molecular weight heparins; however the circulating level of the anti Xa activity associated with demonstrable theraputic efficacy is not yet defined.The availability of a variety of low molecular weight heparins provided us with the opportunity to evaluate the gastrointestinal absorption characteristics of the preparations in the primate.Average molecular weight is only one of a spectrum of variables associated with absorbability, while Xa/APTT ratio differences and non-equivalent structural alterations may be responsible for functional differences in a living test system. Nevertheless, because of the clinical potential it is instructive to evaluate the GI absorbability of several preparations for which we have precise molecular weight data.Preparations: Low molecular weight heparins were prepared by a variety of methods including isolationby alcohol fractionation from broadly polydisperse commercial or crude heparins, depolymerization of commercial or crude heparin and franctionation of depolymerization products.Methods:Molecular weights were established by equilibrium ultracentrifugation and anti Xa activity was assayed by the Yin-Wessler coagulation method. Faste rhesus monkeys weighing 8-13 kg. were anesthestized and intubated with a radio opaque catheter. One cubic centimeter of a heparin preparation in saline was instilled directly into the duodenum. Blood samples assayed for anti Xa activity and thromboelasticity were drawn at periodic intervals from an indwelling femoral catheter.Results:Standard unfractionated heparin was detectable in blood only after one-half hour. The maximum activity for low molecular weight preparations was achieved after one-half to one hour.One fraction demonstrated activity in the plasma after four hours. Thedose response curve for one fraction at half-hour wascuyvilineal between 7 and 16 Mg/Kg.
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Sakdasri, Winatta, Panisara Arnutpongchai, Supasuta Phonsavat et Ruengwit Sawangkeaw. « Response Surface Optimization of Crude Polysaccharides from Grey Oyster Mushroom (Pleurotus sajor-caju (Fr.) Singer) Using Pressurized Hot Water Extraction ». Dans Foods 2021. Basel Switzerland : MDPI, 2021. http://dx.doi.org/10.3390/foods2021-11054.
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Kreismane, Dzidra, Elita Aplocina, Kaspars Naglis-Liepa, Laima Berzina, Olga Frolova et Arnis Lenerts. « Diet optimization for dairy cows to reduce ammonia emissions ». Dans Research for Rural Development 2021 : annual 27th International scientific conference proceedings. Latvia University of Life Sciences and Technologies, 2021. http://dx.doi.org/10.22616/rrd.27.2021.005.
Texte intégralRésumé :
Feeding livestock a balanced diet with a differentiated crude protein (CP) content, depending on the lactation phase can reduce nitrogen emissions from livestock excrement and urine. A higher content of non-starch polysaccharides in livestock diets improves feed absorption in the livestock body and, consequently, nitrogen is emitted more from protein present in livestock manure than from urea acid present in livestock urine. The aim of the study is to calculate the ammonia emission reduction potential in Latvia by optimizing the feeding of dairy cows and ensuring life longevity, as well as provide justification for ammonia emission reduction in dairy farms. Calculations made by using the NorFor Model for optimization of dairy cow (Bos primigenius f. taurus) diets revealed that compared with lowyielding cows, a higher CP content diet fed to high-yielding cows at the beginning of lactation increased the amount of nitrogen (N) in their excrement and urine by 90–180 g d-1. Reducing the CP content in the cow diet by an average of 10 g kg-1 dry matter (DM) during mid-lactation resulted in the same trend. Reducing the CP content in the cow diet during late lactation and the dry period by another 20–30 g kg-1 of DM, N emissions from excrement and urine significantly decreased. Increasing the lifespan of dairy cows also means reducing ammonia emissions from the farm. By increasing the number of lactations per cow on dairy farm, it is possible to reduce the number of heifers per cow. The total reduction of ammonia emissions in Latvia was calculated based on a long-term projection of a decrease of 0.1 heifer per dairy cow. Ammonia emissions could be reduced by 0.051 kt by decreasing the number of heifers by 12.54 thou. at the planned increase in the lifespan of dairy cows by 2030.