Littérature scientifique sur le sujet « CrisprCas9 »
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Articles de revues sur le sujet "CrisprCas9"
Zúñiga Orozco, Andrés. « Tecnología CRISPR-Cas9 : una herramienta aplicable en la agricultura de Costa Rica ». Repertorio Científico 20, no 2 (15 novembre 2018) : 131–38. http://dx.doi.org/10.22458/rc.v20i2.2396.
Texte intégralBarrangou, Rodolphe, et David Bikard. « Guest editorial : CRISPRcas9 : CRISPR-Cas systems : at the cutting edge of microbiology ». Current Opinion in Microbiology 37 (juin 2017) : vii—viii. http://dx.doi.org/10.1016/j.mib.2017.09.015.
Texte intégralInam, Rafia. « Review Article on Anti-Microbial Resistance : Global Approach ». Global Immunological & ; Infectious Diseases Review I, no I (30 décembre 2016) : 36–50. http://dx.doi.org/10.31703/giidr.2016(i-i).04.
Texte intégralOphinni, Youdiil. « CRISPR-Cas9 as a curative drug for chronic viral infection ». BIO Web of Conferences 41 (2021) : 02010. http://dx.doi.org/10.1051/bioconf/20214102010.
Texte intégralJohnson, James, Franziska Linke, Cathy Merry et Beth Coyle. « MEDB-24. Tumour secreted extracellular matrix predicts survival and influences migration and cell death in SHH medulloblastoma 3D models ». Neuro-Oncology 24, Supplement_1 (1 juin 2022) : i110. http://dx.doi.org/10.1093/neuonc/noac079.398.
Texte intégralDontenwill, M., M. Mercier, G. Gillmann, D. Reita, I. Lelong-Rebel, F. Noulet, A. Idbaih et al. « P11.59 Integrin a5 heterogeneous expression in glioblastoma is related to glioma stem cell subpopulations ». Neuro-Oncology 21, Supplement_3 (août 2019) : iii57. http://dx.doi.org/10.1093/neuonc/noz126.205.
Texte intégralBlatov, I. A., A. S. Shchurova, D. Yu Guschin, S. D. Zvereva et A. V. Popova. « CRISPR/Cas-Systems : characteristics and possibilities of use for editing bacterial genomes ». Bacteriology 5, no 2 (2020) : 38–48. http://dx.doi.org/10.20953/2500-1027-2020-2-38-48.
Texte intégralLezon-Geyda, Kimberly, Vincent P. Schulz, Yelena Maksimova et Patrick G. Gallagher. « Altered Splicing from a Mutated Alternate Branch Point Is Common in Severe Alpha-Spectrin Linked Inherited Anemia ». Blood 132, Supplement 1 (29 novembre 2018) : 503. http://dx.doi.org/10.1182/blood-2018-99-117752.
Texte intégralIordache, Dumitrana, Gabriela-Maria Baci, Oana Căpriță, Anca Farkas, Andreea Lup et Anca Butiuc-Keul. « Correlation between CRISPR Loci Diversity in Three Enterobacterial Taxa ». International Journal of Molecular Sciences 23, no 21 (23 octobre 2022) : 12766. http://dx.doi.org/10.3390/ijms232112766.
Texte intégralWu, Mingming, Lixin Du, Ruizao Liu, Caihong Wei, Yayu Wang, Li Yang, Jiafan Liu, Yuqin Wang, Chuduan Wang et Xiaogang Wang. « Double-Muscled Phenotype in Mutant Sheep Directed by the CRISPRCas9 System ». Cloning & ; Transgenesis 07, no 01 (2018). http://dx.doi.org/10.4172/2168-9849.1000161.
Texte intégralThèses sur le sujet "CrisprCas9"
Figueredo, Everthon Fernandes. « Nocaute do gene ipdC no Bacillus sp. (RZ2MS9) com a técnica de CRISPRCas9 e influência sobre a biossíntese do AIA dependente do L-triptofano ». Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-22012019-175701/.
Texte intégralAmong the mechanisms related to the bacterium-plant interaction, the bacterial biosynthesis of indole acetic acid (AIA) plays a fundamental role in the promotion of plant growth, since it is capable of influencing innumerable physiological processes in plants. Different metabolic pathways are used by bacteria for the biosynthesis of IAA, with the indole-3-pyruvic acid (IPyA) pathway being the most commonly described. In this pathway, the indole-3-pyruvate decarboxylase (ipdC) gene has a vital role in the production of IAA using the amino acid L-tryptophan as a precursor. In this context, molecular studies about the metabolic pathways and the genes involved in this process are preponderant for the understanding of the interrelationship of the regulatory pathways with the phytormonium synthesis. The rhizobacterium Bacillus sp. (RZ2MS9) has been showing satisfactory activity in promoting plant growth. The sequencing of its genome pointed to the presence of a wide range of genes related to growth promotion, especially genes encoding auxins. Thus, the objective of the present study was to verify the function of the ipdC gene in the IAA biosynthesis L-tryptophan dependent through the knockout of the ipdC in the plant growth-promoting rhizobateria (PGPR) Bacillus sp. (RZ2MS9). Therefore, the knockout was realized using the CRISPR-Cas9. The knockout of the ipdC gene was efficient, generating disruptive mutants for the said gene. IAA biosynthesis by the ΔipdC strain showed reductions in phytormonium concentrations, according to the growth time, being 87.96% in 24 hours, 88.25% in 48 hours and 58.27% in 72 hours of growth compared to the Wild Type (WT). In addition, the biosynthesis of IAA in the absence of the amino acid L-tryptophan was also evaluated, with no phytormonium synthesis being observed at any growth time, both in the wild type and ΔipdC strain. The present study pioneered the knockout of the ipdC gene in a Bacillus strain using the CRISPR-Cas9. The results obtained contribute to a better understanding of the influence of the ipdC gene and the IPyA pathway in the IAA biosynthesis through the RZ2MS9 strain, and its role in plant growth promoting will be demonstrated in the future.
Inchauspe, Aurore. « De la détection de l'ADNccc par de nouvelles technologies à la preuve de concept de sa dégradation à visée thérapeutique dans des modèles d'infection par le virus de l'hépatite B ». Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1218/document.
Texte intégralHepatitis B virus {HBV) is a major health problem with 250 million chronic carriers, despite the existence of a preventive vaccine. Currently the treatments used are nucleos{t)ide analogues and / or interferon a. Although they efficiently reach a decrease of the viral load, they do not allow the eradication the disease due to the persistence of the cccDNA, the minichromosome of the hepatitis B. This DNA serves as a template for the viral transcription and only a single copy suffice for the infection rebound. However, the techniques used routinely for the quantification of the cccDNA are not sensitive enough to be able to detect low concentrations of this DNA, in particular in biopsies of patients chronically infected and long term treated. ln addition, it is necessary to develop new strategies to target the cccDNA in order to clear the infection. Thus, my thesis work is based on the development of a new technology: the Droplet Digital PCR {ddPCR) to allow the quantification of cccDNA in patient biopsies. This technique allows a gain of 2 log in sensitivity compared to the qPCR technique currently used in routine. lt allowed us to see the presence of this DNA in long-term treated patients even in the presence of interferon. The presence of pgRNA and ChlP experiments also confirmed that the cccDNA was still transcriptionally active.These results confirm the requirement to develop new therapeutics to allow the inactivation or even the elimination of the cccDNA. One of the strategies envisaged is the CRlSPR / Case 9 system. Thus, the following part of this thesis was to develop this system in hepatitis B virus infection models. To reduce off-target effect we tested 8 different guide RNAs incorporated via ribonucleoproteins into HepG2- NTCP cells. Preliminary results have shown that this system can reduce the pool of cccDNA in these cells and open up the possibilities to test this model on PHH and opens interesting perspectives for the development of new treatments
Antunes, Catia S. R. « Malaria parasites : oral and nasal inoculation of mice with iRBC's & ; development of P. falciparum mutants with the CRISPR/cas9 system ». Thesis, Bangor University, 2017. https://research.bangor.ac.uk/portal/en/theses/malaria-parasites-oral-and-nasal-inoculation-of-mice-with-irbcs--development-of-p-falciparum-mutants-with-the-crisprcas9-system(bb845996-c41e-4fe7-9d24-17584b9dade8).html.
Texte intégralSantos, Rafael Miyashiro Nunes dos. « Substituição gênica ortotópica de porco para humano baseada em CRISPR/Cas9 e recombinases para xenotransplante ». Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/5/5168/tde-14112017-153947/.
Texte intégralHumanized pig models are very important for biomedical research, and drugs and treatment development. Not only it is a better model for diseases than smaller animals because of its closer physiology, anatomy, metabolism and life span, it also may provide unlimited organs for transplantation. In spite of all this advantages, inconsistent gene expression in transgenic animals make its generation and evaluation expensive, unpredictable and do not allow proper outcome comparison between different animals. In this report we describe a reproducible technique utilizing the endogenous promoter for generation of a clonal pattern gene replacement protocol (clonal gene transplant) without cell cloning, maintaining the normal gene expression and its regulation. This protocol is reproducible and applicable to more than one gene target, allowing fast generation of transgenic animals cell lines (as low as 14-20 days) and could become the new standard for transgenic large animal generation
MUNAGALA, UDAY. « Deaminases and beyond : pathology, physiology and biotechnology ». Doctoral thesis, 2018. http://hdl.handle.net/2158/1125039.
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