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Articles de revues sur le sujet « Crassostrea gigas – Comportement animal »

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Auteur : Grafiati
Publié le 7 juillet 2024
Mis à jour le 7 juillet 2024

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1

Melo, Emílio Mateus Costa, Simone Sühnel, Francisco Carlos da Silva et Claudio Manoel Rodrigues de Melo. « Induction to tetraploidy in Pacific oysters (Crassostrea gigas) ». Acta Scientiarum. Animal Sciences 44 (10 mai 2022) : e55337. http://dx.doi.org/10.4025/actascianimsci.v44i1.55337.

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As an alternative to the use of cytochalasin B (CB), 6-dimethylamino-purine (6-DMAP) and thermal shock (heat shock by increasing the temperature from 25 to 36ºC) could be used to induce tetraploidy in Pacific oyster (Crassostrea gigas) diploids. Induction was performed by applying shocks after elimination of the first polar corpuscle at the end of meiosis I. Ploidy rates were verified using flow cytometry. Tetraploid larvae were obtained using all inductor (6-DMAP, thermal shock and CB) treatments. No difference in the efficiency of tetraploidy induction was noted among 6-DMAP, thermal shock and CB. The number of D-larvae and their yield, determined by calculating the percentage of well-formed D-larvae in relation to the total number of larvae, was similar (p > 0.05) among the evaluated induction methods. We suggest that 6-DMAP and thermal shock should be used in tetraploidy induction protocols, thereby avoiding the use of CB, which is a harmful agent for both humans and the environment.
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Launey, Sophie, et Dennis Hedgecock. « High Genetic Load in the Pacific Oyster Crassostrea gigas ». Genetics 159, no 1 (1 septembre 2001) : 255–65. http://dx.doi.org/10.1093/genetics/159.1.255.

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Abstract The causes of inbreeding depression and the converse phenomenon of heterosis or hybrid vigor remain poorly understood despite their scientific and agricultural importance. In bivalve molluscs, related phenomena, marker-associated heterosis and distortion of marker segregation ratios, have been widely reported over the past 25 years. A large load of deleterious recessive mutations could explain both phenomena, according to the dominance hypothesis of heterosis. Using inbred lines derived from a natural population of Pacific oysters and classical crossbreeding experiments, we compare the segregation ratios of microsatellite DNA markers at 6 hr and 2–3 months postfertilization in F2 or F3 hybrid families. We find evidence for strong and widespread selection against identical-by-descent marker homozygotes. The marker segregation data, when fit to models of selection against linked deleterious recessive mutations and extrapolated to the whole genome, suggest that the wild founders of inbred lines carried a minimum of 8–14 highly deleterious recessive mutations. This evidence for a high genetic load strongly supports the dominance theory of heterosis and inbreeding depression and establishes the oyster as an animal model for understanding the genetic and physiological causes of these economically important phenomena.
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Moal, J., J. Y. Daniel, D. Sellos, A. Van Wormhoudt et J. F. Samain. « Amylase mRNA expression in Crassostrea gigas during feeding cycles ». Journal of Comparative Physiology B : Biochemical, Systemic, and Environmental Physiology 170, no 1 (1 février 2000) : 21–26. http://dx.doi.org/10.1007/s003600050003.

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Leitão, Alexandra, Catherine Thiriot-Quiévreux, Pierre Boudry et Isabel Malheiro. « A 'G' chromosome banding study of three cupped oyster species : Crassostrea gigas, Crassostrea angulata and Crassostrea virginica (Mollusca : Bivalvia) ». Genetics Selection Evolution 31, no 5 (1999) : 519. http://dx.doi.org/10.1186/1297-9686-31-5-519.

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Kamaishi, Takashi, et Tomoyoshi Yoshinaga. « Detection of Haplosporidium nelsoni in Pacific oyster Crassostrea gigas in Japan. » Fish Pathology 37, no 4 (2002) : 193–95. http://dx.doi.org/10.3147/jsfp.37.193.

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LYNCH, S. A., E. ABOLLO, A. RAMILO, A. CAO, S. C. CULLOTY et A. VILLALBA. « Observations raise the question if the Pacific oyster, Crassostrea gigas, can act as either a carrier or a reservoir for Bonamia ostreae or Bonamia exitiosa ». Parasitology 137, no 10 (14 avril 2010) : 1515–26. http://dx.doi.org/10.1017/s0031182010000326.

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SUMMARYThis study investigated the ability of the Pacific oyster, Crassostrea gigas, to act as a carrier or reservoir of the protistan Bonamia ostreae. Studies were carried out independently in Ireland and in Spain. Naïve C. gigas were exposed to B. ostreae both in the field and in the laboratory via natural exposure or experimental injection. Naïve flat oysters, Ostrea edulis, were placed in tanks with previously exposed C. gigas. Oysters were screened for B. ostreae by examination of ventricular heart smears and by polymerase chain reaction (PCR) screening of tissue samples (gill and/or heart) and shell cavity fluid. PCR-positive oysters were further screened using histology and in situ hybridization (ISH). B. ostreae DNA was detected in the tissues and/or shell cavity fluid of a small number of C. gigas in the field and in the laboratory. B. ostreae-like cells were visualized in the haemocytes of 1 C. gigas and B. ostreae-like cells were observed extracellularly in the connective tissues of 1 other C. gigas. When C. gigas naturally exposed to B. ostreae were held with naïve O. edulis, B. ostreae DNA was detected in O. edulis; however, B. ostreae cells were not visualized. In Spain, B. exitiosa DNA was also detected in Pacific oyster tissues. The results of this study have important implications for C. gigas transfers from B. ostreae-endemic areas to uninfected areas and highlight B. ostreae and B. exitiosa's ability to survive extracellularly and in other non-typical hosts.
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BATISTA, F. M., P. BOUDRY, A. DOS SANTOS, T. RENAULT et F. RUANO. « Infestation of the cupped oysters Crassostrea angulata, C. gigas and their first-generation hybrids by the copepod Myicola ostreae : differences in susceptibility and host response ». Parasitology 136, no 5 (5 mars 2009) : 537–43. http://dx.doi.org/10.1017/s0031182009005691.

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SUMMARYWe studied the prevalence and intensity of the parasitic copepod Myicola ostreae in 2 closely related oysters Crassostrea angulata and C. gigas and their F1 hybrids. The effects on host and host reaction were also analysed to better understand host-parasite relationships between copepods and bivalve molluscs. Full reciprocal crosses were carried out between C. angulata and C. gigas and the progenies were reared in the wild in Ria Formosa Lagoon (Portugal), allowing natural infestation by M. ostreae. Prevalence and intensity were significantly higher in C. angulata than in C. gigas. The parasite level of F1 hybrids was similar to C. angulata and significantly higher than in C. gigas. The results of our study support a hypothesis of dominantly inherited susceptibility to M. ostreae infestation. Moreover, copepods were observed on the gill surface of C. gigas engulfed by a capsule-like structure. Histological analyses revealed that the copepods were surrounded by a massive agglomerate of haemocyte-like cells encircled by a thin layer of fibroblast-like cells. This encapsulation response was not observed in C. angulata or in F1 hybrids. These results suggest that the differential susceptibility to M. ostreae between C. angulata and C. gigas may be ascribed to host defence factors.
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Grijalva-Chon, José Manuel, Reina Castro-Longoria, Tania Lizbeth Enríquez-Espinoza, Alfonso Nivardo Maeda-Martínez et Fernando Mendoza-Cano. « Molecular evidence of the protozoan parasite Marteilia refringens in Crassostrea gigas and Crassostrea corteziensis from the Gulf of California ». Latin American Journal of Aquatic Research 43, no 4 (28 février 2017) : 776–80. http://dx.doi.org/10.3856/vol43-issue4-fulltext-16.

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The search for exotic pathogens related to the outbreaks and in surveillance samplings of the Mexican oyster farms, is a recent activity achieved by academic institutions and state committees for Aquatic Animal Health, with remarkable results. In samples of Crassostrea gigas collected through December 2009, January 2010 and November 2010, and of C. corteziensis in September 2011, the protozoan Marteilia refringens was detected for the first time in the Gulf of California. The carrier oysters were from cultures without abnormal mortality rates, whereby, the use of histology, in situ hybridization and transmission electron microscopy studies are necessary to determine if M. refringens has become established in the Gulf of California oyster cultures. Detection of M. refringens is of great concern to the global oyster farming industry.
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9

Song, Kai. « Genomic Landscape of Mutational Biases in the Pacific Oyster Crassostrea gigas ». Genome Biology and Evolution 12, no 11 (28 juillet 2020) : 1943–52. http://dx.doi.org/10.1093/gbe/evaa160.

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Abstract Mutation is a driving force of evolution that has been shaped by natural selection and is universally biased. Previous studies determined genome-wide mutational patterns for several species and investigated the heterogeneity of mutational patterns at fine-scale levels. However, little evidence of the heterogeneity of mutation rates over large genomic regions was shown. Hence, the mutational patterns of different large-scale genomic regions and their association with selective pressures still need to be explored. As the second most species-rich animal phylum, little is known about the mutational patterns in Mollusca, especially oysters. In this study, the mutational bias patterns are characterized by using whole-genome resequencing data in the Crassostrea gigas genome. I studied the genome-wide relative rates of the pair mutations and found that the predominant mutation is GC -> AT, irrespective of the genomic regions. This analysis reveals that mutational biases were associated with gene expression levels across the C. gigas genome. Genes with higher expression levels and breadth expression patterns, longer coding length, and more exon numbers had relatively higher GC -> AT rates. I also found that genes with larger dN/dS values had relatively higher GC -> AT rates. This work represents the first comprehensive research on the mutational biases in Mollusca species. Here, I comprehensively investigated the relationships between mutational biases with some intrinsic genetic factors and evolutionary indicators and proposed that selective pressures are important forces shaping the mutational biases across the C. gigas genome.
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10

Takahashi, Keisuke G., et Katsuyoshi Mori. « NADPH Oxidase-like Activity in Hemocytes of the Pacific Oyster Crassostrea gigas. » Fish Pathology 35, no 1 (2000) : 15–19. http://dx.doi.org/10.3147/jsfp.35.15.

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Rodríguez-Jaramillo, C., A. M. Ibarra, P. Soudant et E. Palacios. « Comparison of quantitative gonad maturation scales in a temperate oyster (Crassostrea gigas) and a sub-tropical oyster (Crassostrea corteziensis) ». Invertebrate Reproduction & ; Development 61, no 3 (19 avril 2017) : 147–56. http://dx.doi.org/10.1080/07924259.2017.1315341.

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Wang, Xiaotong, Xiaorui Song, Tong Wang, Qihui Zhu, Guoying Miao, Yuanxin Chen, Xiaodong Fang, Huayong Que, Li Li et Guofan Zhang. « Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas ». Current Zoology 59, no 1 (1 février 2013) : 109–15. http://dx.doi.org/10.1093/czoolo/59.1.109.

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Abstract Mollusc shell matrix proteins (SMPs) are important functional components embedded in the shell and play a role in shell formation. A SMP (Pif177) was identified previously from the nacreous layer of the Japanese pearl oyster Pinctada fucata, and its cleavage products (named pfPif97 and pfPif80 proteins) were found to bind to the chitin framework and induce aragonite crystal formation and orient the c axis. In this study, a homologue of pfPif177 was cloned from the mantle of the Pacific oyster Crassostrea gigas, containing the homologue of pfPif97 only and not pfPif80. This finding hints at the large divergence in gene structure between the two species. This homologue (cgPif97) shares characteristics with pfPif97, and suggests that the biological functions of these two proteins may be similar. The expression pattern of cgPif97 in different tissues and development stages indicates that it may play an important role in shell formation of the adult oyster. The morphology of the inner shell surface was affected by injected siRNA of cgPif97 and the calcite laths of the shell became thinner and narrower when the siRNA dose increased, suggesting that the cgPif97 gene plays an important role in calcite shell formation in C. gigas. In conclusion, we found evidence that the Pif177 gene evolved very fast but still retains a similar function among species.
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13

Wang, Y., R. Ren et Z. Yu. « Bioinformatic mining of EST-SSR loci in the Pacific oyster, Crassostrea gigas ». Animal Genetics 39, no 3 (26 février 2008) : 287–89. http://dx.doi.org/10.1111/j.1365-2052.2008.01701.x.

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Dong, Qiaoxiang, Changjiang Huang, Benoit Eudeline, Standish K. Allen et Terrence R. Tiersch. « Systematic factor optimization for sperm cryopreservation of tetraploid Pacific oysters, Crassostrea gigas ». Theriogenology 66, no 2 (juillet 2006) : 387–403. http://dx.doi.org/10.1016/j.theriogenology.2005.12.004.

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15

Aranishi, Futoshi, et Yusuke Iidzuka . « Multiplex PCR Diagnosis for Crassostrea Oyster Discrimination of C. sikamea and C. gigas ». Journal of Fisheries and Aquatic Science 2, no 2 (15 février 2007) : 173–77. http://dx.doi.org/10.3923/jfas.2007.173.177.

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Gomes, Renata Bezerra, Patrick Rafael DYBAS, Francisco Carlos SILVA, Simone SÜHNEL, Marcos Caivano Pedroso ALBUQUERQUE et Claudio Manoel Rodrigues MELO. « Estimates of genetic parameters for growth and survival in pacific oyster (Crassostrea gigas) ». Boletim do Instituto de Pesca 44, no 2 (5 avril 2018) : 337. http://dx.doi.org/10.20950/1678-2305.2018.337.

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Zhang, Enshuo, Zan Li, Bin Li, Jingjing Fu, Yanwei Feng, Guohua Sun, Xiaohui Xu, Cuiju Cui, Weijun Wang et Jianmin Yang. « Investigating the molecular mechanism of sterility in female triploid Pacific oyster (Crassostrea gigas) ». Aquaculture Reports 34 (février 2024) : 101885. http://dx.doi.org/10.1016/j.aqrep.2023.101885.

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Huvet, A., P. Boudry, M. Ohresser, C. Delsert et F. Bonhomme. « Variable microsatellites in the Pacific Oyster Crassostrea gigas and other cupped oyster species ». Animal Genetics 31, no 1 (janvier 2000) : 71–72. http://dx.doi.org/10.1111/j.1365-2052.2000.579-5.x.

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Bai, J., Q. Li, L. F. Kong et R. H. Li. « Characterization of 20 single nucleotide polymorphism markers in the Pacific oyster (Crassostrea gigas) ». Animal Genetics 40, no 6 (décembre 2009) : 1004. http://dx.doi.org/10.1111/j.1365-2052.2009.01949.x.

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Itoh, Naoki, Tadashi Oda, Kazuo Ogawa et Hisatsugu Wakabayashi. « Identification and Development of a Paramyxean Ovarian Parasite in the Pacific Oyster Crassostrea gigas. » Fish Pathology 37, no 1 (2002) : 23–28. http://dx.doi.org/10.3147/jsfp.37.23.

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LANGO-REYNOSO, FABIOLA, NICOLE DEVAUCHELLE, MARCEL LE PENNEC et PHILIPPE-JACQUES HATT. « Elements of reproductive strategy in oysters,Crassostrea gigas, from the “Rade de Brest”, France ». Invertebrate Reproduction & ; Development 36, no 1-3 (septembre 1999) : 141–44. http://dx.doi.org/10.1080/07924259.1999.9652690.

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O’ Reilly, A. J., C. Laide, A. Maloy, S. Hutton, B. Bookelaar, K. O’ Sullivan, S. A. Lynch et S. C. Culloty. « The role of the mussel Mytilus spp. in the transmission of ostreid herpesvirus-1 microVar ». Parasitology 145, no 8 (21 décembre 2017) : 1095–104. http://dx.doi.org/10.1017/s0031182017002244.

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AbstractThe Pacific oyster Crassostrea gigas contributes significantly to global aquaculture; however, C. gigas culture has been affected by ostreid herpesvirus-1 (OsHV-1) and variants. The dynamics of how the virus maintains itself at culture sites is unclear and the role of carriers, reservoirs or hosts is unknown. Both wild and cultured mussels Mytilus spp. (Mytilus edulis, Mytilus galloprovincialis and hybrids) are commonly found at C. gigas culture sites. The objective of this study was to investigate if Mytilus spp. can harbour the virus and if viral transmission can occur between mussels and oysters. Mytilus spp. living at oyster trestles, 400–500 m higher up the shore from the trestles and up to 26 km at non-culture sites were screened for OsHV-1 and variants by all the World Organization for Animal Health (OIE) recommended diagnostic methods including polymerase chain reaction (PCR), quantitative PCR (qPCR), histology, in situ hybridization and confirmation using direct sequencing. The particular primers that target OsHV-1 and variants, including OsHV-1 microVar (μVar), were used in the PCR and qPCR. OsHV-1 μVar was detected in wild Mytilus spp. at C. gigas culture sites and more significantly the virus was detected in mussels at non-culture sites. Cohabitation of exposed wild mussels and naïve C. gigas resulted in viral transmission after 14 days, under an elevated temperature regime. These results indicate that mussels can harbour OsHV-1 μVar; however, the impact of OsHV-1 μVar on Mytilus spp. requires further investigation.
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Zhang, Linlin, Li Li, Fei Xu, Haigang Qi, Xiaotong Wang, Huayong Que et Guofan Zhang. « Fosmid library construction and end sequences analysis of the Pacific oyster,Crassostrea gigas ». Molluscan Research 33, no 1 (février 2013) : 65–73. http://dx.doi.org/10.1080/13235818.2012.754149.

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Haddaji, Najla, Ibtissem Chakroun, Kais Fdhila, Hela Smati, Amina Bakhrouf et Ridha Mzoughi. « Pathogenic Impacts of Bacillus cereus Strains on Crassostrea gigas ». Foodborne Pathogens and Disease 19, no 2 (1 février 2022) : 151–58. http://dx.doi.org/10.1089/fpd.2021.0050.

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Du, Mingyang, Zhuxiang Jiang, Chaogang Wang, Chenchen Wei, Qingyuan Li, Rihao Cong, Wei Wang, Guofan Zhang et Li Li. « Genome-Wide Association Analysis of Heat Tolerance in F2 Progeny from the Hybridization between Two Congeneric Oyster Species ». International Journal of Molecular Sciences 25, no 1 (21 décembre 2023) : 125. http://dx.doi.org/10.3390/ijms25010125.

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As the world’s largest farmed marine animal, oysters have enormous economic and ecological value. However, mass summer mortality caused by high temperature poses a significant threat to the oyster industry. To investigate the molecular mechanisms underlying heat adaptation and improve the heat tolerance ability in the oyster, we conducted genome-wide association analysis (GWAS) analysis on the F2 generation derived from the hybridization of relatively heat-tolerant Crassostrea angulata ♀ and heat-sensitive Crassostrea gigas ♂, which are the dominant cultured species in southern and northern China, respectively. Acute heat stress experiment (semi-lethal temperature 42 °C) demonstrated that the F2 population showed differentiation in heat tolerance, leading to extremely differentiated individuals (approximately 20% of individuals die within the first four days with 10% survival after 14 days). Genome resequencing and GWAS of the two divergent groups had identified 18 significant SNPs associated with heat tolerance, with 26 candidate genes located near these SNPs. Eleven candidate genes that may associate with the thermal resistance were identified, which were classified into five categories: temperature sensor (Trpm2), transcriptional factor (Gata3), protein ubiquitination (Ube2h, Usp50, Uchl3), heat shock subfamily (Dnajc17, Dnaja1), and transporters (Slc16a9, Slc16a14, Slc16a9, Slc16a2). The expressional differentiation of the above genes between C. gigas and C. angulata under sublethal temperature (37 °C) further supports their crucial role in coping with high temperature. Our results will contribute to understanding the molecular mechanisms underlying heat tolerance, and provide genetic markers for heat-resistance breeding in the oyster industry.
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Ramesh, Kirti, Marian Y. Hu, Frank Melzner, Markus Bleich et Nina Himmerkus. « Intracellular pH regulation in mantle epithelial cells of the Pacific oyster, Crassostrea gigas ». Journal of Comparative Physiology B 190, no 6 (20 août 2020) : 691–700. http://dx.doi.org/10.1007/s00360-020-01303-3.

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Abstract Shell formation and repair occurs under the control of mantle epithelial cells in bivalve molluscs. However, limited information is available on the precise acid–base regulatory machinery present within these cells, which are fundamental to calcification. Here, we isolate mantle epithelial cells from the Pacific oyster, Crassostrea gigas and utilise live cell imaging in combination with the fluorescent dye, BCECF-AM to study intracellular pH (pHi) regulation. To elucidate the involvement of various ion transport mechanisms, modified seawater solutions (low sodium, low bicarbonate) and specific inhibitors for acid–base proteins were used. Diminished pH recovery in the absence of Na+ and under inhibition of sodium/hydrogen exchangers (NHEs) implicate the involvement of a sodium dependent cellular proton extrusion mechanism. In addition, pH recovery was reduced under inhibition of carbonic anhydrases. These data provide the foundation for a better understanding of acid–base regulation underlying the physiology of calcification in bivalves.
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Itoh, Naoki, Tadashi Oda, Tomoyoshi Yoshinaga et Kazuo Ogawa. « DNA Probes for Detection of Marteilioides chungmuensis from the Ovary of Pacific Oyster Crassostrea gigas ». Fish Pathology 38, no 4 (2003) : 163–69. http://dx.doi.org/10.3147/jsfp.38.163.

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Aguirre-Macedo, M. L., et C. R. Kennedy. « Patterns in metazoan parasite communities of some oyster species ». Journal of Helminthology 73, no 4 (avril 1999) : 283–88. http://dx.doi.org/10.1017/s0022149x00700599.

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Metazoan parasite communities of Crassostrea gigas and Ostrea edulis from Great Britain, Crassostrea virginica from Mexico, and Saccostrea commercialis from Australia are described and summarized in terms of species composition, species richness, total number of individuals and dominance. Metazoan parasite communities in all host species were composed of turbellarians and the metacercarial stage of digeneans, with the exception of S. commercialis where only metacercariae were found. Arthropods, including one copepod and one mite species, were present only in British oyster species. All metazoan parasite communities of oysters had few species and low density of individuals. Richest communities were found in C. virginica at both component and infracommunity level. The least diverse component community occurred in S. commercialis. Infracommunities in O. edulis and S. commercialis never exceeded one species per host. The host response against parasites is suggested as the principal factor responsible for depauperate parasite communities of oysters. Environmental factors characteristic of tropical latitudes are likely to have enhanced both the number of species and the densities of parasites per host in the infracommunities of C. virginica.
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Meng, Jie, Linlin Zhang, Baoyu Huang, Li Li et Guofan Zhang. « Comparative analysis of oyster (Crassostrea gigas) immune responses under challenge by differentVibriostrains and conditions ». Molluscan Research 35, no 1 (16 octobre 2014) : 1–11. http://dx.doi.org/10.1080/13235818.2014.919696.

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Meyer, E., et D. T. Manahan. « Gene expression profiling of genetically determined growth variation in bivalve larvae (Crassostrea gigas) ». Journal of Experimental Biology 213, no 5 (12 février 2010) : 749–58. http://dx.doi.org/10.1242/jeb.037242.

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Andreyeva, Aleksandra Yu, Ekaterina S. Kladchenko, Oksana Y. Vyalova et Tatiana A. Kukhareva. « Functional Characterization of the Pacific Oyster, Crassostrea gigas (Bivalvia : Ostreidae), Hemocytes Under Normoxia and Short-Term Hypoxia ». Turkish Journal of Fisheries and Aquatic Sciences 21, no 03 (28 décembre 2020) : 125–33. http://dx.doi.org/10.4194/1303-2712-v21_3_03.

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Hemolymph cellular composition, morphology and functional properties of the Pacific oyster (Crassostrea gigas) hemocytes were studied. Three hemocyte types (agranulocytes, hyalinocytes and granulocytes) were described in hemolymph. The morphology of each type was characterized by light microscopy and flow cytometry. Agranular cells (agranulocytes and hyalinocytes) were the dominant type of cells in hemolymph; their number was 86.7±2.7% of total cell count. Under hypoxia the number of agranulocytes increased (37.4% for control group versus 95.3% for hypoxic probes), whereas granulocyte and hyalinocyte number decreased up to 3.9% and 0.7% in hypoxic specimens respectively. The spontaneous ROS production decreased in each hemocyte type after exposure to hypoxia. Low dissolved oxygen did not influence hemocyte proliferation and mortality level.
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Appleyard, S. A., P. D. Kube et R. D. Ward. « Estimation of genetic parameters in cultured Pacific oysters (Crassostrea gigas) grown in Tasmania, based on animal models ». Aquaculture 272 (2007) : S240. http://dx.doi.org/10.1016/j.aquaculture.2007.07.021.

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Prasetiya, Fiddy S., Priscilla Decottignies, Laurent Barillé, Romain Gastineau, Boris Jacquette, Amandine Figiel, Michèle Morançais, Réjean Tremblay, Jean-Luc Mouget et Bruno Cognie. « Cell size-based, passive selection of the blue diatom Haslea ostrearia by the oyster Crassostrea gigas ». Journal of Molluscan Studies 83, no 2 (9 mars 2017) : 145–52. http://dx.doi.org/10.1093/mollus/eyx012.

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Prudence, M., J. Moal, P. Boudry, J. Y. Daniel, C. Quere, F. Jeffroy, C. Mingant et al. « An amylase gene polymorphism is associated with growth differences in the Pacific cupped oyster Crassostrea gigas ». Animal Genetics 37, no 4 (août 2006) : 348–51. http://dx.doi.org/10.1111/j.1365-2052.2006.01481.x.

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Taris, N., R. P. Lang, P. W. Reno et M. D. Camara. « Transcriptome response of the Pacific oyster (Crassostrea gigas) to infection withVibrio tubiashiiusing cDNA AFLP differential display ». Animal Genetics 40, no 5 (octobre 2009) : 663–77. http://dx.doi.org/10.1111/j.1365-2052.2009.01894.x.

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Gutierrez, A. P., J. Symonds, N. King, K. Steiner, T. P. Bean et R. D. Houston. « Potential of genomic selection for improvement of resistance to ostreid herpesvirus in Pacific oyster ( Crassostrea gigas ) ». Animal Genetics 51, no 2 (30 janvier 2020) : 249–57. http://dx.doi.org/10.1111/age.12909.

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Lacoste, A., S. K. Malham, A. Cueff, F. Jalabert, F. Gelebart et S. A. Poulet. « Evidence for a form of adrenergic response to stress in the mollusc Crassostrea gigas ». Journal of Experimental Biology 204, no 7 (1 avril 2001) : 1247–55. http://dx.doi.org/10.1242/jeb.204.7.1247.

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Catecholamines and pro-opiomelanocortin (POMC)-derived peptides, some of the central regulators of the stress-response systems of vertebrates, are also present in invertebrates. However, studies are needed to determine how these hormones participate in the organisation of neuroendocrine stress-response axes in invertebrates. Our present work provides evidence for the presence of an adrenergic stress-response system in the oyster Crassostrea gigas. Noradrenaline and dopamine are released into the circulation in response to stress. Storage and release of these hormones take place in neurosecretory cells presenting morphological and biochemical similarities with vertebrate chromaffin cells. Both in vivo and in vitro experiments showed that applications of the neurotransmitters acetylcholine or carbachol caused no significant release of noradrenaline or dopamine. Moreover, the nicotinic antagonists hexamethonium and α -bungarotoxin and the muscarinic antagonist atropine caused no significant inhibition of catecholamine release in stressed oysters. Adrenocorticotropic hormone (ACTH) induced a significant release of noradrenaline, but the release of dopamine in response to ACTH was not significant. These results suggest that, unlike that of vertebrates, the adrenergic stress-response system of oysters is not under the control of acetylcholine and that other factors, such as the neuropeptide ACTH, might control this system.
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Lee, R. J., et R. Silk. « Sources of variation of Escherichia coli concentrations in bivalve molluscs ». Journal of Water and Health 11, no 1 (17 décembre 2012) : 78–83. http://dx.doi.org/10.2166/wh.2012.114.

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Bivalve molluscs can concentrate contaminants, including pathogenic microorganisms, from the water column during their normal filter-feeding activity. In the European Union, the risk of human and animal faecal contamination in bivalves is estimated by determining the concentration of Escherichia coli in time-series samples from production areas. A structured field study was undertaken to determine the extent to which such concentrations varied between sites, sampling occasions and shellfish species and to determine the residual variability of the method. E. coli was enumerated in three species of bivalve mollusc (Crassostrea gigas, Mytilus spp. and Pecten maximus) co-located in each of three geographically separate commercial shellfisheries. The data were subjected to analysis of variance (ANOVA). This showed that the effects of site, sampling occasion, species and site/sampling occasion interaction were all significant. The proportion of variation due to site was markedly greater than that due to other factors. Post-ANOVA analysis showed that the concentration of E. coli in P. maximus was significantly higher than in the other two species. Mytilus spp. and C. gigas exhibited comparable levels of E. coli. The observed standard deviation of the most probable number method in the study was 0.33 log10.
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Lynch, S. A., S. Lepée-Rivero, R. Kelly, E. Quinn, A. Coghlan, B. Bookelaar, E. Morgan, J. A. Finarelli, J. Carlsson et S. C. Culloty. « Detection of haplosporidian protistan parasites supports an increase to their known diversity, geographic range and bivalve host specificity ». Parasitology 147, no 5 (15 novembre 2019) : 584–92. http://dx.doi.org/10.1017/s0031182019001628.

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AbstractHaplosporidian protist parasites are a major concern for aquatic animal health, as they have been responsible for some of the most significant marine epizootics on record. Despite their impact on food security, aquaculture and ecosystem health, characterizing haplosporidian diversity, distributions and host range remains challenging. In this study, water filtering bivalve species, cockles Cerastoderma edule, mussels Mytilus spp. and Pacific oysters Crassostrea gigas, were screened using molecular genetic assays using deoxyribonucleic acid (DNA) markers for the Haplosporidia small subunit ribosomal deoxyribonucleic acid region. Two Haplosporidia species, both belonging to the Minchinia clade, were detected in C. edule and in the blue mussel Mytilus edulis in a new geographic range for the first time. No haplosporidians were detected in the C. gigas, Mediterranean mussel Mytilus galloprovincialis or Mytilus hybrids. These findings indicate that host selection and partitioning are occurring amongst cohabiting bivalve species. The detection of these Haplosporidia spp. raises questions as to whether they were always present, were introduced unintentionally via aquaculture and or shipping or were naturally introduced via water currents. These findings support an increase in the known diversity of a significant parasite group and highlight that parasite species may be present in marine environments but remain undetected, even in well-studied host species.
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Wang, Hebing, Ben Yang, Xin Li, Qi Li et Shikai Liu. « Screening of bacterial pathogens associated with mass summer mortality of the Pacific oyster, Crassostrea gigas, in China ». Aquaculture Reports 20 (juillet 2021) : 100672. http://dx.doi.org/10.1016/j.aqrep.2021.100672.

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Ong, M. C., N. A. M. Shazili, D. Menier et A. W. M. Effendy. « Levels of Trace Elements in Tissue of Ostrea edulis and Crassostrea gigas from Quiberon Bay, Brittany, France ». Journal of Fisheries and Aquatic Science 8, no 2 (15 février 2013) : 378–87. http://dx.doi.org/10.3923/jfas.2013.378.387.

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Collin, H., A. L. Meistertzheim, E. David, D. Moraga et I. Boutet. « Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions ». Journal of Experimental Biology 213, no 23 (12 novembre 2010) : 4010–17. http://dx.doi.org/10.1242/jeb.048033.

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MACINTYRE, E. A., C. G. EARNHART et S. L. KAATTARI. « Host oyster tissue extracts modulate in vitro protease expression and cellular differentiation in the protozoan parasite, Perkinsus marinus ». Parasitology 126, no 4 (avril 2003) : 293–302. http://dx.doi.org/10.1017/s003118200200286x.

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Perkinsus marinus is responsible for a chronic disease (Dermo) of the Eastern oyster, Crassostrea virginica. In order to simulate the in vivo environment more closely, a chemically defined medium (JL-ODRP-3) was supplemented with tissue homogenate extracts or plasma from oysters possessing varying degrees of susceptibility to P. marinus infection. In media supplemented with extracts from highly susceptible oysters (C. virginica), P. marinus cells secreted elevated amounts of a set of low molecular weight serine proteases (LMP: 30–45 kDa) as assessed by enhanced digestion within gelatin-substrate SDS–PAGE gels. Oyster species of low susceptibility (C. gigas and C. ariakensis) did not exhibit this ability to upregulate P. marinus LMP expression. Oyster extract supplementation also led to pronounced changes in P. marinus cellular morphology, such that the cells were comparable to those observed within naturally infected oysters.
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Yurchenko, O. V., V. I. Radashevsky et A. A. Reunov. « Ultrastructural study of spermatogenesis in the Pacific oyster Crassostrea gigas (Bivalvia : Ostreidae) from the Sea of Japan ». Invertebrate Zoology 7, no 1 (décembre 2010) : 55–69. http://dx.doi.org/10.15298/invertzool.07.1.04.

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Cardona-Costa, J., F. García-Ximénez et F. J. Espinós. « Definition of fusion medium and electric parameters for efficient zygote electrofusion in the Pacific oyster (Crassostrea gigas) ». Theriogenology 74, no 5 (septembre 2010) : 828–34. http://dx.doi.org/10.1016/j.theriogenology.2010.04.007.

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Suquet, M., A. Le Mercier, F. Rimond, C. Mingant, P. Haffray et C. Labbe. « Setting tools for the early assessment of the quality of thawed Pacific oyster (Crassostrea gigas) D-larvae ». Theriogenology 78, no 2 (juillet 2012) : 462–67. http://dx.doi.org/10.1016/j.theriogenology.2012.02.014.

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Favrel, P., M. P. Dubos, B. Bernay, J. Pasquier, J. Schwartz, B. Lefranc, L. Mouret, G. Rivière, J. Leprince et A. Bondon. « Structural and functional characterization of an egg-laying hormone signaling system in a lophotrochozoan – The pacific oyster (Crassostrea gigas) ». General and Comparative Endocrinology 346 (janvier 2024) : 114417. http://dx.doi.org/10.1016/j.ygcen.2023.114417.

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Kang, Jung-Ha, Hyun Jeong Lim, Hyun-Soek Kang, Jung-Mee Lee, Sumy Baby et Jong-Joo Kim. « Development of Genetic Markers for Triploid Verification of the Pacific Oyster, <italic>Crassostrea gigas</italic> ; ». Asian-Australasian Journal of Animal Sciences 26, no 7 (1 juillet 2013) : 916–20. http://dx.doi.org/10.5713/ajas.2013.13108.

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Lynch, Sharon A., Rachel Breslin, Babette Bookelaar, Tawut Rudtanatip, Kanokpan Wongprasert et Sarah C. Culloty. « Immunomodulatory and Antiviral Effects of Macroalgae Sulphated Polysaccharides : Case Studies Extend Knowledge on Their Importance in Enhancing Shellfish Health, and the Control of a Global Viral Pathogen Ostreid Herpesvirus-1 microVar ». Polysaccharides 2, no 2 (1 avril 2021) : 202–17. http://dx.doi.org/10.3390/polysaccharides2020014.

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Macroalgae are the primary source of non-animal sulphated polysaccharides (SPs) in the marine environment with fucoidans derived from brown algae (Phaeophyta) and carrageenans from red algae (Rhodophyta). Much research has been carried out on SP effects on Asian shrimp species (genera Penaeus and Metapenaeus) but their effect on commercially important bivalve mollusc species is limited and in Pacific oyster Crassostrea gigas is unknown. Knowledge of their impact on bivalve pathogens and Palaemon shrimp is unknown. The objectives of this study were to assess the effects of Fucus vesiculosus (Phaeophyta), Mastocarpus stellatus (Rhodophyta) and algal derivatives (fucoidan and κ-carrageenan) on C. gigas performance, and on ostreid herpesvirus-1 microvar (OsHV-1 μVar) and bacteria Vibrio spp. development. Both pathogens have been associated with significant oyster mortalities and economic losses globally. The effects of sulphated galactan from Gracilaria fisheri (Rhodophyta) on European common prawn Palaemon serratus, an important fishery species, was also assessed. Findings indicate a rapid and prolonged increase in total blood cell count, lysozyme (enzyme that destroys pathogens), and a difference in the ratio of blood cell types in treated individuals compared to their control counterparts. A significantly lower OsHV-1 μVar prevalence was observed in treated oysters and κ-carrageenan was found to suppress viral replication (loads), while OsHV-1 μVar was not detected in the fucoidan treated oysters from Day 8 of the 26-day trial. No antibacterial effect was observed however, the oysters did not succumb to vibriosis. These findings contribute further knowledge to macroalgae sulphated polysaccharide biotherapeutic properties, their twofold effect on animal health and viral suppression.
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Bigot, L., I. Beets, M. P. Dubos, P. Boudry, L. Schoofs et P. Favrel. « Functional characterization of a short neuropeptide F-related receptor in a lophotrochozoan, the mollusk Crassostrea gigas ». Journal of Experimental Biology 217, no 16 (19 juin 2014) : 2974–82. http://dx.doi.org/10.1242/jeb.104067.

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