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1
Camus, Emmanuel, et Arnaud Martrenchar. « Infection expérimentale de zébus guyanais avec Trypanosoma vivax ». Revue d’élevage et de médecine vétérinaire des pays tropicaux 43, no 4 (1 avril 1990) : 467–72. http://dx.doi.org/10.19182/remvt.8761.
Texte intégralRésumé :
La souche guyanaise de Trypanosoma vivax est pathogène pour le zébu guyanais de type Brahman : l'infection expérimentale de 19 bovins, agés d'un an, s'est traduite par de la fièvre (peu élevée et transitoire), une chute de l'hématocrite et une perte de poid rapide et marquée (10 à 17 kg en un mois par rapport aux 16 témoins). D'autres symptômes accompagne n t la parasitémie : diarrhée, ganglions hématiques sur le cou et le flanc, larmoiement, asthénie. Malgré un traitement trypanocide instauré un mois après l'infection, un bovin est mort et les autres n'ont pas retrouvé leur poids 3 mois plus tard. La sensibilité particulière des animaux au moment du sevrage est discutée ainsi que ses implications pour la lutte contre la trypanosomose.
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Ezenwosu, C., N. W. Anizoba, C. C. Nwoga, M. O. Onodugo, M. C. Ogwuegbu, F. U. Udeh, A. V. Mmoegbunam et A. E. Onyimonyi. « Carcass and organ characteristics of broiler finisher chickens fed dietary inclusion levels of Telfairia occidentalis leaf meal ». Nigerian Journal of Animal Production 49, no 2 (8 mars 2022) : 149–56. http://dx.doi.org/10.51791/njap.v49i2.3474.
Texte intégralRésumé :
Searching for a non-conventional and less competitive plant feed sources such as Telfairia occidentalis leaf meal to serve as an alternative in poultry nutrition will solve the problem of scarcity and high cost of convectional feed sources facing poultry production today. The objective of this study was to investigate the effect of dietary inclusion levels of Telfairia occidentalis leaf meal (TOLM) on the carcass and organ characteristics of finisher broiler chickens. A total of 120 Anak strain broiler birds at 28 days of age of mixed sexes were used. The birds at the completion of their fourth week were randomly assigned to four dietary treatments, with 30 birds per treatment. Each treatment was replicated thrice with 10 bird per replicate. The TOLM was included in feed and fed ad libitum to the birds in a completely randomized experimental design. The inclusion levels of TOLM in the feed were: T (control 1 diet) = 0 % TOLM. T = 0.5 % TOLM. T = 1 % TOLM and T = 1.5 % TOLM. The following 2 3 4 carcass and organ parameters were measured: dressed carcass, breast muscle, thigh muscle, wing, shank, head, neck, visceral, liver, intact gizzard, and empty gizzard and heart weights. Lengths of small intestines of the birds were also measured. The results obtained showed significant (p<0.05) differences in the mean values for dressed carcass weight, shank weight, breast muscle weight, thigh muscle, weight of head and neck. For organ parameters measured, visceral, liver, intact gizzard and heart weights values were not significant (p>0.05), while values for small intestine length and empty gizzard weights among the treatments were significant (p<0.05). Birds on TOLM diet improved significantly (p<0.05) in carcass and organ parameters measured compared with the control birds, while among the treatment groups, T birds recorded the highest(p<0.05) values in all the carcass and organ 4 parameters measured followed by T . Generally, there was an observed increase in carcass 3 and organ parameters as TOLM level in the diets increased. In conclusion 1.5 % level of Telfairia occidentalis leaf meal was recommended due to its influence on carcass and organ parameters.
La recherche de sources d'alimentation végétales non conventionnelles et moins compétitives telles que la farine de feuilles de Telfairia occidentalis pour servir d'alternative à la nutrition de la volaille résoudra le problème de la rareté et du coût élevé des sources d'alimentation par convection auxquelles la production de volaille est confrontée aujourd'hui. L'objectif de cette étude était d'étudier l'effet des niveaux d'inclusion alimentaire de la farine de feuilles de Telfairia occidentalis (FFTO) sur les caractéristiques de la carcasse et des organes des poulets à griller en finition. Un total de 120 poulets à griller de souche Anak âgés de 28 jours de sexes mixtes ont été utilisés. Les oiseaux à la fin de leurquatrième semaine ont été assignés au hasard à quatre traitements diététiques, avec 30 oiseaux par traitement. Chaque traitement a été répété trois fois avec 10 oiseaux par répétition. Le FFTO a été inclus dans les aliments et distribué ad libitum aux oiseaux dans une conception expérimentale complètement randomisée. Les niveaux d'inclusion de FFTO dans l'aliment étaient : T (régime témoin) = 0 % de FFTO. T = 0,5 % FFTO. T = 1 % FFTO 1 2 3 et T = 1,5 % FFTO. Les paramètres suivants de la carcasse et des organes ont été mesurés : 4 carcasse habillée, muscle de la poitrine, muscle de la cuisse, aile, jarret, tête, cou, viscéral, foie, gésier intact et poids du gésier et du cœur vides. La longueur des intestins grêles des oiseaux a également été mesurée. Les résultats obtenus ont montré des différences significatives (p<0,05) dans les valeurs moyennes du poids de la carcasse habillée, du poids du jarret, du poids du muscle de la poitrine, du muscle de la cuisse, du poids de la tête et du cou. Pour les paramètres d'organes mesurés, les valeurs de poids des viscères, du foie, du gésier intact et du cœur n'étaient pas significatives (p>0,05), tandis que les valeurs de longueur de l'intestin grêle et de poids du gésier vide parmi les traitements étaient significatives (p<0,05). Les oiseaux sous régime FFTO ont amélioré de manière significative (p<0,05) les paramètres de la carcasse et des organes mesurés par rapport aux oiseaux témoins, tandis que parmi les groupes de traitement, les oiseaux T ont enregistré les 4 valeurs les plus élevées (p<0,05) pour tous les paramètres de la carcasse et des organes mesurés, suivis de T . En général, on a observé une augmentation des paramètres de la 3 carcasse et des organes à mesure que le niveau de FFTO dans les régimes augmentait. En conclusion, un niveau de 1,5 % de farine de feuilles de Telfairia occidentalis a été recommandé en raison de son influence sur les paramètres de la carcasse et des organes.
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Ezenwosu, C., N. W. Anizoba, C. C. Nwoga, M. O. Onodugo, M. C. Ogwuegbu, F. U. Udeh, A. V. Mmoegbunam et A. E. Onyimonyi. « Carcass and organ characteristics of broiler finisher chickens fed dietary inclusion levels of Telfairia occidentalis leaf meal ». Nigerian Journal of Animal Production 49, no 3 (9 juin 2022) : 160–67. http://dx.doi.org/10.51791/njap.v49i3.3546.
Texte intégralRésumé :
Searching for a non-conventional and less competitive plant feed sources such as Telfairia occidentalis leaf meal to serve as an alternative in poultry nutrition will solve the problem of scarcity and high cost of convectional feed sources facing poultry production today. The objective of this study was to investigate the effect of dietary inclusion levels of Telfairia occidentalis leaf meal (TOLM) on the carcass and organ characteristics of finisher broiler chickens. A total of 120 Anak strain broiler birds at 28 days of age of mixed sexes were used. The birds at the completion of their fourth week were randomly assigned to four dietary treatments, with 30 birds per treatment. Each treatment was replicated thrice with 10 birds per replicate. The TOLM was included in feed and fed ad libitum to the birds in a completely randomized experimental design. The inclusion levels of TOLM in the feed were: T1 (control diet) = 0 % TOLM. T2 = 0.5 % TOLM. T 3 = 1 % TOLM and T4 = 1.5 % TOLM. The following carcass and organ parameters were measured: dressed carcass, breast muscle, thigh muscle, wing, shank, head, neck, visceral, liver, intact gizzard, and empty gizzard and heart weights. Lengths of small intestines of the birds were also measured. The results obtained showed significant (p<0.05) differences in the mean values for dressed carcass weight, shank weight, breast muscle weight, thigh muscle, weight of head and neck. For organ parameters measured, visceral, liver, intact gizzard and heart weights values were not significant (p>0.05), while values for small intestine length and empty gizzard weights among the treatments were significant (p<0.05). Birds on TOLM diet improved significantly (p<0.05) in carcass and organ parameters measured compared with the control birds, while among bhbbthe treatment groups, T4 birds recorded the highest(p<0.05) values in all the carcass and organ parameters measured followed by T3 . Generally, there was an observed increase in carcass and organ parameters as TOLM level in the diets increased. In conclusion 1.5 % level of Telfairia occidentalis leaf meal was recommended due to its influence on carcass and organ parameters.
La recherche de sources d'alimentation végétales non conventionnelles et moins compétitives telles que la farine de feuilles de Telfairia occidentalis pour servir d'alternative à la nutrition de la volaille résoudra le problème de la rareté et du coût élevé des sources d'alimentation par convection auxquelles la production de volaille est confrontée aujourd'hui. L'objectif de cette étude était d'étudier l'effet des niveaux d'inclusion alimentaire de la farine de feuilles de Telfairia occidentalis (FFTO) sur les caractéristiques de la carcasse et des organes des poulets à griller en finition. Un total de 120 poulets à grillerde souche Anak âgés de 28 jours de sexes mixtes ont été utilisés. Les oiseaux à la fin de leur quatrième semaine ont été assignés au hasard à quatre traitements diététiques, avec 30 oiseaux par traitement. Chaque traitement a été répété trois fois avec 10 oiseaux par répétition. Le FFTO a été inclus dans les aliments et distribué ad libitum aux oiseaux dans une conception expérimentale complètement randomisée. Les niveaux d'inclusion de FFTO dans l'aliment étaient : T (régime témoin) = 0 % de FFTO. T = 0,5 % FFTO. T = 1 % FFTO 1 2 3 et T = 1,5 % FFTO. Les paramètres suivants de la carcasse et des organes ont été mesurés : 4 carcasse habillée, muscle de la poitrine, muscle de la cuisse, aile, jarret, tête, cou, viscéral, foie, gésier intact et poids du gésier et du cœur vides. La longueur des intestins grêles des oiseaux a également été mesurée. Les résultats obtenus ont montré des différences significatives (p<0,05) dans les valeurs moyennes du poids de la carcasse habillée, du poids du jarret, du poids du muscle de la poitrine, du muscle de la cuisse, du poids de la tête et du cou. Pour les paramètres d'organes mesurés, les valeurs de poids des viscères, du foie, du gésier intact et du cœur n'étaient pas significatives (p>0,05), tandis que les valeurs de longueur de l'intestin grêle et de poids du gésier vide parmi les traitements étaient significatives (p<0,05). Les oiseaux sous régime FFTO ont amélioré de manière significative (p<0,05) les paramètres de la carcasse et des organes mesurés par rapport aux oiseaux témoins, tandis que parmi les groupes de traitement, les oiseaux T ont enregistré les 4 valeurs les plus élevées (p<0,05) pour tous les paramètres de la carcasse et des organes mesurés, suivis de T . En général, on a observé une augmentation des paramètres de la 3 carcasse et des organes à mesure que le niveau de FFTO dans les régimes augmentait. En conclusion, un niveau de 1,5 % de farine de feuilles de Telfairia occidentalis a été recommandé en raison de son influence sur les paramètres de la carcasse et des organes.
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Cardoso, Marinice O., Ademar P. de Oliveira, Walter E. Pereira et Adailson P. de Souza. « Growth, nutrition and yield of eggplant as affected by doses of cattle manure and magnesium thermophosphate plus cow urine ». Horticultura Brasileira 27, no 3 (septembre 2009) : 307–13. http://dx.doi.org/10.1590/s0102-05362009000300008.
Texte intégralRésumé :
The stem growth, nutrition, and fruit yield of eggplant grown with different doses of cattle manure (CM) and magnesium thermophosphate (MT) were studied, according to the Central Composite Matrix of Box (8.3-518; 8.3-3,018; 48.3-518; 48.3-3,018; 0.0-1,768; 56.6-1,768; 28.3-0.0; 28.3-3,536; 28.3-1,768). Potassium sulfate (KS) was also used (24 g plant-1), as well as a cow urine (COU) solution (10 L 100 L-1 H2O, 500 mL plant-1), applied five times in topdressing. Two additional treatments were used: MT without COU and triple superphosphate + urea (TS plus Ur), both with nutrient levels (CM, P2O5, and KS) corresponding to the combination 28.3-1,768. The experimental design was blocks at random, with three replications. CM doses improved plant height (PH) (29.1 t ha-1 of CM; 69.84 cm) and stem diameter (37.8 t ha-1; 19.8 mm), fitting into a quadratic model. However, due to the positive interaction between CM and MT, PH increased up to 86.87 cm when the same CM dose was combined with 3,536 kg ha-1 of MT. CM doses had also a quadratic effect over number of fruits, fruit production per plant, average fruit weight (AFW), and yield, with respective maximum values corresponding to 53.4 t ha-1 (13 fruit), 55.8 t ha-1 (2,822 g plant-1), 47.8 t ha-1 (210 g fruit-1), and 48.8 t ha-1 (34.3 t ha-1), without significant effects of MT. N and P foliar contents did not change with treatments. CM doses increased K, Ca, and S, and decreased Mg foliar contents. But, concerning Ca, was observed negative interaction of MT. COU showed no effect over the characteristics analyzed. The effect of TS + Ur was larger than that of MT + COU on PH and production indexes, except for AFW, but did not differ from them as to macronutrient foliar contents.
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Roma Rejeki Nami Saragih, Christian Neni Purba et Asima Rohana Sinaga. « The Effect Of Jigsaw Teachnique On Students’ Writing Skill At Eeventh Grade Of SMA Negeri 5 Pematang Siantar ». Jurnal Ilmu Pendidikan dan Sosial 2, no 4 (22 novembre 2023) : 547–55. http://dx.doi.org/10.58540/jipsi.v2i4.461.
Texte intégralRésumé :
This research was carried out to find whether or not the effect of Jigsaw technique on students’ writing skill in Analytical Exposition Text at eeventh grade of SMA Negeri 5 Pematang Siantar. The research methodoogy used in this research was a quasi-experimental research. There were two classes taken as the samples of this research, class XI IPS 2 as the experimental class and class XI IPS 4 as the contro class. The mean score in experimental class from pre-test to post-test was 51,66 and 72,22. The mean score in contro class from pre-test to post-test was 54,44 and 61,66. The improvement as the experimental class got in post-test was 20,56 and as the contro class got in post-test was 7,22. The sum of standard deviation in experimental class was 2037,50 and in contro class was 3271,88. The data were colected by administering pre-test and post-test. The research findings showed that there was significant effect by using Jigsaw technique on students’ writing skill. It can be seen from the testing hypothesis, it showed that the t-test value (6,51) was higher than the t-table value (1,98) at leve of significance 5% with degree of freedom (df) was 70. This are by the score of post-test in both classes. The average post-test score in experimental class was 72,22 was higher than the average post-test score in contro class which was 51,66. It can be concluded that there is a significant difference in the writing skill between the students who are taught by using Jigsaw technique on Students’ writing skill in Analytical Exposition Texts and those who are taught without using it.
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Kaur, Surinder, Stephen C. Alley, Matt Szapacs, Amanda Wilson, Eugene Ciccimaro, Dian Su, Neil Henderson et al. « 2021 White Paper on Recent Issues in Bioanalysis : Mass Spec of Proteins, Extracellular Vesicles, CRISPR, Chiral Assays, Oligos ; Nanomedicines Bioanalysis ; ICH M10 Section 7.1 ; Non-Liquid & ; Rare Matrices ; Regulatory Inputs (Part 1A – Recommendations on Endogenous Compounds, Small Molecules, Complex Methods, Regulated Mass Spec of Large Molecules, Small Molecule, PoC & ; Part 1B - Regulatory Agencies' Inputs on Bioanalysis, Biomarkers, Immunogenicity, Gene & ; Cell Therapy and Vaccine) ». Bioanalysis 14, no 9 (mai 2022) : 505–80. http://dx.doi.org/10.4155/bio-2022-0078.
Texte intégralRésumé :
The 15th edition of the Workshop on Recent Issues in Bioanalysis (15th WRIB) was held on 27 September to 1 October 2021. Even with a last-minute move from in-person to virtual, an overwhelmingly high number of nearly 900 professionals representing pharma and biotech companies, contract research organizations (CROs), and multiple regulatory agencies still eagerly convened to actively discuss the most current topics of interest in bioanalysis. The 15th WRIB included 3 Main Workshops and 7 Specialized Workshops that together spanned 1 week in order to allow exhaustive and thorough coverage of all major issues in bioanalysis, biomarkers, immunogenicity, gene therapy, cell therapy and vaccines. Moreover, in-depth workshops on biomarker assay development and validation (BAV) (focused on clarifying the confusion created by the increased use of the term “Context of Use – COU”); mass spectrometry of proteins (therapeutic, biomarker and transgene); state-of-the-art cytometry innovation and validation; and, critical reagent and positive control generation were the special features of the 15th edition. This 2021 White Paper encompasses recommendations emerging from the extensive discussions held during the workshop, and is aimed to provide the bioanalytical community with key information and practical solutions on topics and issues addressed, in an effort to enable advances in scientific excellence, improved quality and better regulatory compliance. Due to its length, the 2021 edition of this comprehensive White Paper has been divided into three parts for editorial reasons. This publication (Part 1A) covers the recommendations on Endogenous Compounds, Small Molecules, Complex Methods, Regulated Mass Spec of Large Molecules, Small Molecule, PoC. Part 1B covers the Regulatory Agencies' Inputs on Bioanalysis, Biomarkers, Immunogenicity, Gene & Cell Therapy and Vaccine. Part 2 (ISR for Biomarkers, Liquid Biopsies, Spectral Cytometry, Inhalation/Oral & Multispecific Biotherapeutics, Accuracy/LLOQ for Flow Cytometry) and Part 3 (TAb/NAb, Viral Vector CDx, Shedding Assays; CRISPR/Cas9 & CAR-T Immunogenicity; PCR & Vaccine Assay Performance; ADA Assay Comparabil ity & Cut Point Appropriateness) are published in volume 14 of Bioanalysis, issues 10 and 11 (2022), respectively.
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Garcia, Dalia L., et Tamar H. Gollan. « 15 Different Languages, Different Linguistic Markers : Predicting Which Bilinguals will Develop Alzheimer's Disease with Spontaneous Spoken Language ». Journal of the International Neuropsychological Society 29, s1 (novembre 2023) : 226–27. http://dx.doi.org/10.1017/s135561772300334x.
Texte intégralRésumé :
Objective:Spontaneous speech undergoes subtle but significant changes years before the onset of Alzheimer's dementia (AD). In monolinguals, these changes, or linguistic markers of AD, include the use of syntactically simpler structures, reduced lexical diversity, reduced semantic detail/specificity, and increased disfluencies (Ostrand & Gunstad, 2020; Slegers et al., 2018; Venneri et al., 2018). No studies have examined if bilinguals exhibit similar changes in their language output prior to diagnosis of AD though this question has important clinical relevance and can also shed light on which cognitive abilities decline first with AD pathology. Of particular interest, changes in semantic representations might affect both languages (because semantics are shared between the two), but changes in executive control might be more prominent in the nondominant language (because of interference from the dominant language).Participants and Methods:Seventeen older Spanish-English bilinguals completed an interview in which they described a picture in each language and answered a series of questions beginning with "warm-up" questions and progressing to questions that elicited higher level language (e.g., defending an opinion). All participants were considered cognitively healthy at the time of testing, but 8 participants later developed Alzheimer's Disease (i.e., converters) on average after 4.1 (SD=2.5) years, while 9 matched controls remained cognitively healthy on average for 5.7 (SD=3.6) years (for as long as they were followed). Converters and controls were matched for age, education, language proficiency, and cognitive status at the time of testing. Language samples were transcribed word for word and analyzed using the Systematic Analysis of Language (Miller & Iglesias, 2012).Results:Converters and controls were compared on measures of syntactic complexity, lexical diversity, abandoned utterances, errors, and disfluencies. In the dominant language, the number of different words (using a moving window average; a measure of lexical diversity), showed promise for classifying who would eventually convert (Area Under the Curve = 77), though the difference between converters and controls was significant only in a 1-tailed test (t(15)=-1.96, p=.034). In the nondominant language, converters showed a higher percent of Maze words compared to controls (2-tailed t (15) = 2.27, p = 0.039). Mazes combine repetitions, filled pauses, and revisions. Further exploration of Maze subcomponents revealed that filled pauses and revisions produced no differences between groups in either language (all ps3.18), but converters produced more repetitions (e.g., "the the boy" or "the cou-counter") than controls, (2-tailed t-tests in both languages were significant; ps <.03). However, variability in repetitions was high, making it less sensitive in the ROC analysis.Conclusions:Changes in bilinguals' spoken language output occur years before diagnosis, in agreement with literature on monolinguals. However, in bilinguals, the two languages may be differentially affected by cognitive changes. The dominant language may be more sensitive for discriminating groups possibly reflecting semantic decline and decreased ability to quickly access a variety of words. But changes in the nondominant language reveal a broader nature of cognitive deficits in prodromal AD, including decreased circumlocution abilities to avoid disfluencies when faced with word-finding difficulties.
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Polovyi, V. M., L. A. Yashchenko, H. F. Rovna et B. V. Huk. « Biological CO2 cycle and organic carbon balance in maize (Zea mays) – soybean (Glycine hispida (Moench) Maxim.) agrocenosis in sod-podzolic soil ». Scientific Journal Grain Crops 7, no 1 (2023) : 162–69. http://dx.doi.org/10.31867/2523-4544/0272.
Texte intégralRésumé :
Topicality. Today, the search for ways to accumulate organic carbon and restore soil fertility, as well as increase crop yield, is a topical issue for the sod-podzolic soils of Western Polissia. Purpose. To determine the intensity of CO2 emission and the organic carbon balance on sod-podzolic soil at different fertilizer rates in the maize-soybean link against the background of chemical amelioration with incorporation of by-products. Methods. We used such methods as a stationary field trial, comparative and calculation method to determine the accumulated and emitted CO2 by plants, analytical and calculation methods to determine the organic carbon balance. Results. The CO2 emissions from organic matter mineralization amounted to 5.01–5.45 t/ha by the recommended fertilizer rate and calculated by the normative method on the background of dolomite and limestone powder, which was 23.4–34.2 % higher than the control (without fertilizers). The CO2 emission into the atmosphere through plant mass mineralization, depending on fertilization and chemical amelioration, was in the range of 18.6–24.7 t/ha and exceeded the control (without fertilizers) and the background of 1.0 Hh (hydrolytic acidity) CaMg(CO3)2 by 1.2–2.0 times, which is associated with improved soil conditions, higher by-products mass and CO2 accumulation by plants. The highest amount of CO2 was accumulated by maize (64.8–65.0 t/ha) and soybean (15.0–15.8 t/ha) at combination of the fertilizer rate calculated by the normative method with microfertilizers on the background of dolomite flour. The application of calculated fertilizer rates on the background of 1.0 Hh CaMg(CO3)2 provided an advantage in the formation of organic carbon in the soil, which formed a positive balance of 0.12 and 0.15 t/ha. In the variant without fertilizers and chemical amelioration, the ratio of total CO2 emissions into the atmosphere per 1 t of grain yield in the maize-soybean link was 4.65 and 4.62 units, while the application of the fertilizer rate calculated by the normative method against the background of 1.0 Hh CaMg(CO3)2 decreased to 3.78 and 3.89 units, respectively. Conclusions. For increasing the maize and soybean productivity on sod-podzolic loamy sandy soil, incorporation of plant mass into the soil with applying the fertiliser rates calculated by the standard method against the background of 1.0 Nh CaMg(CO3)2 is an effective method to control soil degradation that ensures the inclusion of additional organic carbon into the cycle, which is aimed at its fixation by forming a deficit-free balance of 0.12 and 0.15 t/ha. Key words: emission, organic carbon, productivity, fertilization, land amelioration, plant mass
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Odetola, O. M., et O. O. Adetola. « Growth response, carcass analysis and sensory evaluation of meat from broiler chicken served acidified water ». Nigerian Journal of Animal Production 49, no 1 (25 février 2022) : 284–92. http://dx.doi.org/10.51791/njap.v49i1.3427.
Texte intégralRésumé :
Due to the emergence of drugs resistance microorganisms, side effects of antimicrobials and harmful residual toxicity effects of drugs observed in food chain, there is an increasing trend towards the use of alternatives for the general health maintenance. This study was carried out using 135 day old arbor acre chicks to evaluate the growth response, carcass and sensory evaluation of meat obtained from broiler chickens served two different organic acids. The chicks after brooded for a period of seven days were randomly distributed into five treatments group of 27 chicks per treatment replicated thrice with 9 birds per replicate in a completely randomized design (CRD). Treatment 1 served as the control without oral inclusion of organic acid, T had 2% citric acid, T had 4% citric acid, T had 2% acetic acid 2 3 4 and T had 4% acetic acid respectively while all administration was done two weeks at 5 starter phase and two weeks at finisher phase. parameter measured includes feed intake, weight gain, feed conversion ratio and mortality percentage while two birds per replicate making 6 per treatment were randomly selected, slaughtered and dissected for carcass, meat quality and sensory evaluation respectively. Result obtained showed no significant (P >0.05) difference in all growth parameters measured. Significant (P< 0.05) differences were obtained on the mean value recorded for drumstick, wing, neck, head and gizzard among all carcass parameters measured. Higher (P< 0.05) values for drumstick was obtained on T2 (11.17) followed by T and T (10.65 and 10.67) while the least values was recorded for T and 1 4 3 T (9.54 and 10.10). The same trend was observed for gizzard. Significant (P< 0.05) 5 differences were obtained in cooking loss recorded on breast muscle and on chilling loss recorded on thigh muscle and drumstick across the treatment for all parameters measured for meat quality attributes. Sensory evaluation results revealed similarities in the values obtained for colour, appearance, flavor, texture, taste and overall acceptance. Conclusively, the inclusion of citric and acetic acid orally for broilers had no detrimental effect on growth performance but enhance the quality of the carcass.
En raison de l'émergence de micro-organismes résistants aux médicaments, des effets secondaires des antimicrobiens et des effets toxiques résiduels nocifs des médicaments observés dans la chaîne alimentaire, il existe une tendance croissante à l'utilisation d'alternatives pour le maintien de la santé générale. Cette étude a été réalisée en utilisant des poussins âgés de 135 jours pour évaluer la réponse de croissance, la carcasse et l'évaluation sensorielle de la viande obtenue à partir de poulets à griller servis avec deux acides organiques différents. Les poussins après avoir été couvés pendant une période de sept jours ont été répartis au hasard dans cinq groupes de traitements de 27 poussins par traitement répétés trois fois avec 9 oiseaux par répétition dans une conception complètement randomisée (CCR). Le traitement 1 a servi de contrôle sans inclusion orale d'acide organique, T avait 2 % d'acide citrique, T avait 4 % d'acide citrique, T avait 2 % d'acide 2 3 4 acétique et T avait 4 % d'acide acétique, respectivement, tandis que toute l'administration 5 était effectuée deux semaines à la phase de démarrage et deux semaines en phase finisher. Le paramètre mesuré comprend l'apport alimentaire, le gain de poids, l'indice de conversion alimentaire et le pourcentage de mortalité, tandis que deux oiseaux par répétition, soit 6 par traitement, ont été sélectionnés au hasard, abattus et disséqués pour la carcasse, la qualité de la viande et l'évaluation sensorielle, respectivement. Le résultat obtenu n'a montré aucune différence significative (P > 0,05) dans tous les paramètres de croissance mesurés. Des différences significatives (P < 0,05) ont été obtenues sur la valeur moyenne enregistrée pour le pilon, l'aile, le cou, la tête et le gésier parmi tous les paramètres de carcasse mesurés. Les valeurs les plus élevées (P < 0,05) pour le pilon ont été obtenues sur T2 (11,17) suivi de T1 et T4 (10,65 et 10,67) tandis que les valeurs les plus faibles ont été enregistrées pour T3 et T5 (9,54 et 10,10). La même tendance a été observée pour le gésier. Des différences significatives (P< 0,05) ont été obtenues dans la perte à la cuisson enregistrée sur le muscle de la poitrine et dans la perte au froid enregistrée sur le muscle de la cuisse et le pilon tout au long du traitement pour tous les paramètres mesurés pour les attributs de qualité de la viande. Les résultats de l'évaluation sensorielle ont révélé des similitudes dans les valeurs obtenues pour la couleur, l'apparence, la saveur, la texture, le goût et l'acceptation globale. En conclusion, l'inclusion d'acide citrique et acétique par voie orale pour les poulets de chair n'a eu aucun effet néfaste sur les performances de croissance mais a amélioré la qualité de la carcasse.
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Kumar Das, Dibash. « CAR-T Cells to Control Multiple Myeloma Growth ». Oncology Times 46, no 3 (mars 2024) : 36. http://dx.doi.org/10.1097/01.cot.0001009944.34160.61.
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PASTER, NACHMAN, RIVKA BARKAI-GOLAN et MOSHE CALDERON. « Control of T-2 Toxin Production Using Atmospheric Gases1 ». Journal of Food Protection 49, no 8 (1 août 1986) : 615–17. http://dx.doi.org/10.4315/0362-028x-49.8.615.
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When the fungus Fusarium tricinctum NRRL 3299 was grown under atmospheres enriched with 50% CO2 and above (given in combination with 20% O2), T-2 production was reduced to 4.0 μg/45 ml of medium, as compared with 21.2 μg/45 ml of medium produced in an atmosphere of air. At 60% CO2/20% O2 and 80% CO2/20% O2, a significant reduction in fungal growth was also observed. The possibility of using controlled atmospheres as a means for mycotoxin control is discussed.
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Beau, Aurélien, Denis Bossard et Sarah Gebeile-Chauty. « Les appareils orthodontiques collés doivent-ils être déposés systématiquement avant un examen d’imagerie par résonance magnétique (IRM) ? » L'Orthodontie Française 88, no 2 (juin 2017) : 131–38. http://dx.doi.org/10.1051/orthodfr/2017004.
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Objectifs : Les appareils orthodontiques sont souvent déposés avant les examens d’imagerie par résonance magnétique (IRM) car ils sont réputés pour produire des artefacts. Le but de cette étude était de trouver les indications exactes de dépose de différents types d’appareils fixes lors de la visualisation de quatre zones spécifiques de la tête et du cou. Matériel et méthode : 60 patients nécessitant un examen IRM de la tête pour raisons médicales se sont portés volontaires pour cette étude. Un appareil fixe parmi quatre types d’appareils (boîtiers en acier inoxydable, boîtiers en titane, boîtiers en céramique à gorge métallique et fils de contention en acier inoxydable) a été assigné à chaque patient. Chaque patient a subi deux examens à 1,5 T : avec un «échantillon de cire vide» et avec un échantillon de cire contenant l’appareil. Les arcs n’ont pas été étudiés car leur dépose avant un examen est aisée. Deux radiologues ont évalué les images de chaque patient pour chacune des zones anatomiques étudiées : sinus maxillaire, cavité buccale, articulations temporo-mandibulaires et fosse cérébrale postérieure. Résultats : Les boîtiers en acier inoxydable ont rendu la totalité des images ininterprétables (100 %). Les boîtiers en titane, les boîtiers en céramique à gorge métallique et les fils de contention en acier inoxydable ont causé des artefacts seulement au niveau de la cavité buccale (pour 20 %, 16,65 % et 86,65 % des sujets). Conclusion : Ces résultats montrent que les boîtiers en céramique à gorge métallique et les boîtiers en titane ne doivent pas toujours être déposés avant un examen d’IRM de la tête et du cou, selon la zone anatomique étudiée. Les fils de contention métalliques ne devraient être déposés que si la cavité buccale est étudiée. Les boîtiers en acier inoxydable devraient être systématiquement déposés avant un examen d’IRM de la tête et du cou.
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Menger, Dirk Jan. « The T‐graft to control nasal length and nasal tip position ». Clinical Otolaryngology 46, no 5 (3 juin 2021) : 1127–30. http://dx.doi.org/10.1111/coa.13787.
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Porichis, Filippos, et Daniel E. Kaufmann. « HIV-specific CD4 T cells and immune control of viral replication ». Current Opinion in HIV and AIDS 6, no 3 (mai 2011) : 174–80. http://dx.doi.org/10.1097/coh.0b013e3283454058.
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Veloso, A. C. R., H. F. Lopes, L. F. X. Santos, V. S. Martins Júnior, S. A. Fonseca, T. A. X. Santos, A. D. Matias, R. T. Careli, E. R. Duarte et T. G. S. Braz. « Elephant grass silage inoculated with cellulolytic fungi isolated from rumen ». Arquivo Brasileiro de Medicina Veterinária e Zootecnia 75, no 5 (octobre 2023) : 981–92. http://dx.doi.org/10.1590/1678-4162-12984.
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ABSTRACT The objective was to evaluate the inoculation with Aspergillus terreus and/or Trichoderma longibrachiatum on fermentation, chemical and microbiological composition of elephant grass ‘Cameroon’ silage (Cenchrus purpureus). Treatments were A. terreus at 105 colony forming units (CFU)/g (AT15), T. longibrachiatum at 105 CFU/g (TL20), a mixture of both at 105 CFU/g (MIX), and a control group without inoculation (CONTR). The design was completely randomized with seven replicates. The MIX silage was most stable, while CONTR, AT15, and TL20, had lower dry matter losses. There was no effect of inoculation in the chemical composition of silages. Only MIX silage (4.40) had pH above the minimum of 4.2 for humid grass silage and above the control (4.05). Bacteria from Diplococcus genus was identified at the opening of TL20 and CONTR silages. After air exposure, the population of rods, Lactobacillus, and total lactic acid bacteria was higher in theTL20 and MIX. The inclusion of a T. longibrachiatum and A. terreus mixture increases dry mater loss and silage pH. T. longibrachiatum was more efficient in maintaining populations of total lactic acid bacteria after opening; therefore, this strain has potential as an additive for elephant grass ‘Cameroon’ silage.
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Appay, Victor, et Maria C. Iglesias. « Antigen sensitivity and T-cell receptor avidity as critical determinants of HIV control ». Current Opinion in HIV and AIDS 6, no 3 (mai 2011) : 157–62. http://dx.doi.org/10.1097/coh.0b013e3283453dfd.
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Hartigan-OʼConnor, Dennis J., Lauren A. Hirao, Joseph M. McCune et Satya Dandekar. « Th17 cells and regulatory T cells in elite control over HIV and SIV ». Current Opinion in HIV and AIDS 6, no 3 (mai 2011) : 221–27. http://dx.doi.org/10.1097/coh.0b013e32834577b3.
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Roth, Z., et D. Kalo. « 177 EXPOSURE OF BOVINE OOCYTES TO A RESIDUAL CONCENTRATION OF MONO-(2-ETHYLHEXYL) PHTHALATE FURTHER IMPAIRS BLASTOCYST GENE EXPRESSION ». Reproduction, Fertility and Development 29, no 1 (2017) : 197. http://dx.doi.org/10.1071/rdv29n1ab177.
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Di-(2-ethylhexyl) phthalate and its metabolite, mono-(2-ethylhexyl) phthalate (MEHP), are reproductive toxicants. We recently documented a residual concentration of MEHP (~20 nM) and a 2-fold lower level of oestradiol in preovulatory-follicular fluid aspirated from cows gavaged with di-(2-ethylhexyl) phthalate (100 mg/kg/day; 3 days). To examine the effects of these changes on oocyte developmental competence, cumulus-oocyte complexes (COC; n = 3290; 14 replicates) were in vitro matured (22 h) in standard oocyte maturation media at 38.5°C and 5% CO2. Oocyte maturation media was supplemented with MEHP (0, 20, and 1000 nM) dissolved in dimethyl sulfoxide (0.01% vol/vol), with or without oestradiol (0 or 2000 ng mL−1). The COC were then fertilized (18 h) and further cultured in KSOM at 38.5°C, 5% CO2 and 5% O2, for 7 days. Matured oocytes (n = 20/sample; 4 replicates/group) and blastocysts (n = 4 embryos/sample; 4 replicates/group) were collected. Samples were then underwent mRNA isolation, followed by real-time PCR to analyse the expression of selected genes (Cyc-1, Mt-co1, Atp5b, Pou5f1, Sox2, and Dnmt3b) associated with early embryonic development. Gene expression was quantified and analysed by LightCycler®96 software; the ΔΔCt method was used to calculate the relative expression; 2 genes (Ywhaz and Sdha) were used as internal reference. Statistical analysis was performed by one-way ANOVA followed by Student’s t-test. Findings revealed that maturation with oestradiol and 20 nM MEHP did not affect cleavage or blastocyst formation rate (80.3 ± 1.6 and 16.3 ± 2.2, respectively); however, maturation with 1000 nM decreased the proportion of oocytes that cleaved (70.6 ± 5.8 v. 83.2 ± 2.2, respectively; P < 0.01) and those developed to blastocysts, relative to the control (10.3 ± 1.2 v. 17.2 ± 1.2, respectively; P < 0.04). On the other hand, maturation with 20 or 1000 nM MEHP without oestradiol did not affect cleavage rate, but reduced proportion of developing blastocyst relative to control (15.9 ± 1.6, P < 0.09; 13.9 ± 1.5, P < 0.02; v. 20.6 ± 1.9; respectively). In addition, co-culture of COC with oestradiol and 1000 nM MEHP increased the expression of Cyc-1 and Mt-co1 in matured oocytes (P < 0.05); co-culture of COC with 20 or 1000 nM MEHP increased the expression of Cyc-1, Atp5b, and Dnmt3b in blastocysts (P < 0.05). However, culture with 1000 nM MEHP and without oestradiol increased the expression of Dnmt3b in matured oocytes but reduced the expression of Cyc-1, Mt-co1, Atp5b, Pou5f1, and Dnmt3b in blastocysts (P < 0.05). Although not significant, a similar pattern of gene expression was recorded for blastocysts developed from oocytes matured with 20 nM MEHP, and without oestradiol. The findings emphasise the potential risk associated with phthalate exposure: maturation with low MEHP concentrations deleteriously affected oocyte developmental competence, impaired gene expression in blastocysts developed from MEHP-treated oocytes indicated a carryover effect, and impaired expression of genes associated with early embryonic development suggested that these embryos are of inferior quality.
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Hersperger, Adam R., Stephen A. Migueles, Michael R. Betts et Mark Connors. « Qualitative features of the HIV-specific CD8+ T-cell response associated with immunologic control ». Current Opinion in HIV and AIDS 6, no 3 (mai 2011) : 169–73. http://dx.doi.org/10.1097/coh.0b013e3283454c39.
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Flaig, Thomas W., Matthew R. Smith, Fred Saad, Dana E. Rathkopf, Peter F. A. Mulders, Eric Jay Small, Neal D. Shore et al. « Treatment patterns after abiraterone acetate in patients (pts) with metastatic castration-resistant prostate cancer (mCRPC) : Post hoc analysis of COU-AA-302. » Journal of Clinical Oncology 34, no 2_suppl (10 janvier 2016) : 168. http://dx.doi.org/10.1200/jco.2016.34.2_suppl.168.
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168 Background: Treatment patterns of mCRPC have changed substantially in the last few years. In COU-AA-302 (chemotherapy-naïve men with mCRPC), abiraterone acetate plus prednisone (AA) significantly improved radiographic progression-free survival and overall survival vs placebo plus prednisone (P). There is limited information about treatment patterns after AA. In this post hoc analysis of pts in the AA treatment arm who progressed, we characterized subsequent therapy after pts discontinued study drug. Methods: In COU-AA-302, 546 pts were randomized and received AA. Treatment patterns of pts receiving ≥ 1 subsequent therapy for mCRPC after progressing on AA were collected retrospectively and source verified. Results: As of March 2014, 8% (44/546) of pts continued on AA; 67% (365/546) received ≥ 1 subsequent therapy for mCRPC. 36% (194/546) and 15% (83/546) of pts had ≥ 2 and ≥ 3 subsequent therapies, respectively. 80% (435/543) of pts in the P arm had ≥ 1 subsequent therapy. Most frequent subsequent therapy in AA pts was taxane chemotherapy (docetaxel [DOC], cabazitaxel [CBZ]), androgen signaling–directed therapy (AA, enzalutamide [ENZ], ketoconazole [KETO]), and immunotherapy (sipuleucel-T [SIP-T]) (Table). Among AA pts who received DOC as first subsequent therapy, median age was 69 years; median duration of DOC post-AA (n = 261) was 3 months (IQR: 0.95-5.7); and PSA progression was the most common reason for discontinuation. Among AA pts with baseline PSA and ≥ 1 post-baseline PSA value, 40% (40/100) had ≥ 50% PSA decline (27/100 confirmed responses) with first subsequent DOC chemotherapy. Conclusions: This post hoc analysis indicates that treatment with subsequent therapy was common (67% and 80%) in pts with chemotherapy-naïve mCRPC who progressed with AA or P, respectively. PSA decline suggests antitumor activity in pts who progressed with AA and received subsequent DOC. Despite limitations of a retrospective analysis, these data support further assessment of subsequent therapy following AA treatment for mCRPC. Clinical trial information: NCT00887198. [Table: see text]
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Abukari, Ammal, et Prince Cobbinah. « Can Biochar Made from Rice Husk Affect Savanna Soils’ pH, Electrical Conductivity, and Soil Respiration ? » Turkish Journal of Agriculture - Food Science and Technology 12, no 6 (10 juin 2024) : 978–83. http://dx.doi.org/10.24925/turjaf.v12i6.978-983.6741.
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Biochar is now gaining awareness as a sustainable tool for soil health improvement, boosting carbon (C) storage and the enhancement of nutrient cycling in agricultural soils. This study assesses the effects of biochar on soil respiration, pH, and electrical conductivity (EC) in savanna soils over a 45-day incubation trail in the laboratory. Four different biochar treatments (0, 2, 4, and 6 t/ha) were used in the study. The treatments were established at 26°C, and after 2, 5, and 10 days, the CO2 levels were recorded. After incubation for 0, 5, 10, and 45 days, the EC and pH were assessed. As the rate of application of biochar increased, the rate of CO2 evolution increased as well. During the first two days of incubation, the CO2 evolution rate rose by a value of 129 at 2 t/ha biochar, 146 at 4 t/ha biochar, and 168 ug CO2/g soil/d at 6 t/ha biochar above the 0 t/ha biochar. Following five days of incubation, the amounts of CO2 evolution that were higher than the control were 99 with 2 t/ha, 116 with 4 t/ha, and 120 ug CO2/g soil/d with 6 t/ha of biochar. The increase in CO2 evolution above the control treatment at 10 days of incubation was 61 with 2 t/ha, 79 with 4 t/ha, and 87 ug CO2/g soil/d with 6 t/ha of biochar. Analogously, rising patterns in CO2 emissions were noted. Throughout the whole incubation period, the biochar treatments' soil EC and pH were greater than those of the control treatment. After applying biochar, there were increases in the evolution of CO2, however after 10 days of incubation, the percentage of C evolved from the addition of biochar decreased as the rates of biochar increased. At two t/ha, four t/ha, and six t/ha, the percentage C developed was 1.74 %, 1.66%, and 0.82% of the applied biochar C, respectively. Although the CO2 evolved ratio to the total amount of biochar C typically reduced with increasing biochar rates, this study shows that the addition of biochar increases soil respiration, EC, and pH.
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Simons, Brenna C., et Spyros A. Kalams. « The potential role of epitope-specific T-cell receptor diversity in the control of HIV replication ». Current Opinion in HIV and AIDS 2, no 3 (mai 2007) : 177–82. http://dx.doi.org/10.1097/coh.0b013e3280ef692f.
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Crispe, I. Nicholas. « Frequency of T-Cell Progenitors in Nude Mice ». Developmental Immunology 4, no 4 (1995) : 257–61. http://dx.doi.org/10.1155/1995/89597.
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The hypothesis that prothymocytes are distinct from and regulated independently of multilineage hemopoietic progenitors was tested by enumeration of these two cell populations in normal versus congenitally athymic (nude) mice. The absence of a thymus and of peripheral T cells in nude mice had no effect on the frequency of either multilineage progenitors (day 12 CFU-S) or prothymocytes (CFU-T), suggesting that there is no feedback regulation of CFU-T frequency. Thymus seeding from the bone marrow is therefore likely to be regulated by the availability of niches for prothymocyte maturation, rather than by feedback control of prothymocyte production.
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Zhou, Min, Xinyu Zhang, Biying Pan, Jiaqi Zhu, Xiaoxiao Qian, Xian Li, Kangkang Xu, Bin Tang et Can Li. « The Endogenous Metabolic Response of Tribolium castaneum under a High Concentration of CO2 ». Agriculture 12, no 7 (7 juillet 2022) : 979. http://dx.doi.org/10.3390/agriculture12070979.
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High carbon dioxide concentrations can effectively control most storage pests. To estimate the toxicity effect of high concentrations of CO2, four different concentrations of CO2 (25% CO2, 50% CO2, 75% CO2, and 95% CO2) were used to treat Tribolium castaneum, and the biochemical (carbohydrate content and gene expression level) and physiological (mortality, pupation, eclosion rate, and weight) features of insects submitted to different treatments with CO2 were evaluated. The T. castaneum mortality rate was 50% in approximately 2 days when exposed to a treatment with 95% CO2. When the CO2 concentration exceeded 75%, the pupation rate and eclosion rate of T. castaneum seriously declined. Higher than 25% CO2 concentrations resulted in a lower weight and shrunken body size of T. castaneum. It was further found that different CO2 concentration treatments all influenced the levels of the three carbohydrate contents in T. castaneum. In addition, according to the detection of trehalose metabolism pathway-related genes, T. castaneum mainly responds to stress factors via high expression of TPS, TRE1-2, and TRE1-3. Our results enrich the evaluation of the toxicity effect of CO2 treatment on grain storage pests, providing a basis for further improving the method of regulating grain storage to control insect pests.
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Migueles, Stephen A., John C. Tilton et Mark Connors. « Qualitative host factors associated with immunological control of HIV infection by CD8 T cells ». Current Opinion in HIV and AIDS 1, no 1 (janvier 2006) : 28–33. http://dx.doi.org/10.1097/01.coh.0000194108.14601.69.
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Sutthisa, Waraporn, Aranya Popranom, Atchara Taddeetrakool et Surasak Khankhum. « Development of Trichoderma Formulation and Application to Control Durian Anthracnose Disease ». Trends in Sciences 21, no 1 (1 novembre 2023) : 7276. http://dx.doi.org/10.48048/tis.2024.7276.
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Anthracnose is a plant fungal disease that damages both quantitative and qualitative crop yields. Biological control of plant disease is an alternative method enabling reduced use of chemicals. The objective of this research was to develop a formulation of Trichoderma for controlling plant anthracnose disease. The efficacy of T. asperellum MSU007 in controlling Colletotrichum sp. by dual culture technique showed that T. asperellum MSU007 was able to inhibit the mycelium growth of Colletotrichum sp. with 50.76 - 74.85 % inhibition. Three formulations of T. asperellum MSU007 were developed. The viability of T. asperellum MSU007 in the formulation after drying at 45 °C showed that the viabilities of formulation 2 and 3 were not significantly different at 5.26×108 and 5.30×108 cfu/g, respectively, while that of formulation 1, the viability of T. asperellum MSU007 was reduced to 3.73×106 cfu/g. After storage at room temperature for 30 days, formulations 2 and 3 did not result in significantly different viability of T. asperellum MSU007 at 4.40×108 and 4.90×108 cfu/g, respectively, and after 90 days of storage at room temperature, it was found that only formulation 3 did not decrease T. asperellum MSU007 viability at 4.33×108 cfu/g. The efficacy of Trichoderma bioproduct in inhibiting anthracnose pathogens on durian leaves was tested. It was found that spraying formulation 3 after inoculation of Colletotrichum sp. resulted in the least anthracnose severity at 15.67 ± 4.04 %, which was significantly different from other treatments including the use of carbendazim. However, it is necessary to further study the efficacy of Trichoderma formulation in the inhibition of durian anthracnose pathogens in field conditions in order to be able to use this bioproduct in future disease control. HIGHLIGHTS This research developed an effective Trichoderma bioproduct to control the durian anthracnose causative agent Colletotrichum spp. which has sufficient potential to be developed and applied in the control of this disease, replacing the use of chemicals in the future. GRAPHICAL ABSTRACT
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Bhattacharyya, Shaown, Charles R. Crain, Benjamin Goldberg et Gaurav D. Gaiha. « Features of functional and dysfunctional CD8+ T cells to guide HIV vaccine development ». Current Opinion in HIV and AIDS 18, no 5 (18 juillet 2023) : 257–63. http://dx.doi.org/10.1097/coh.0000000000000812.
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Purpose of review CD8+ T cell responses are a key component of the host immune response to human immunodeficiency virus (HIV) but vary significantly across individuals with distinct clinical outcomes. These differences help inform the qualitative features of HIV-specific CD8+ T cells that we should aim to induce by vaccination. Recent findings We review previous and more recent findings on the features of dysfunctional and functional CD8+ T cell responses that develop in individuals with uncontrolled and controlled HIV infection, with particular emphasis on proliferation, cytotoxic effector function, epitope specificity, and responses in lymph nodes. We also discuss the implications of these findings for both prophylactic and therapeutic T cell vaccine development within the context of T cell vaccine trials. Summary The induction of HIV specific CD8+ T cell responses is an important goal of ongoing vaccine efforts. Emerging data on the key features of CD8+ T cell responses that distinguish individuals who spontaneously control from those with progressive disease continues to provide key guidance.
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Woodworth, Joshua S., Dennis Christensen et Peter Andersen. « Parenteral-Mucosal prime-boost with a TB subunit vaccine enhances early lung T cells against aerosol M. tuberculosis infection with transient improved protection. » Journal of Immunology 198, no 1_Supplement (1 mai 2017) : 73.5. http://dx.doi.org/10.4049/jimmunol.198.supp.73.5.
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Abstract Background T cell mediated immunity is critical to control M.tuberculosis (Mtb) infection and tuberculosis (TB) disease. However, Mtb actively delays the adaptive T cell response after initial infection. Thus, a cornerstone of TB vaccine development is to overcome this delay and recruit T cells for direct contact with infected lung phagocytes to control early bacterial growth. Given parenterally, the H56:CAF01 subunit vaccine results in an early CD4 T cell response in Mtb-infected lungs and significant reduction in CFU. Here, we investigate mucosal prime-boost delivery of H56:CAF01 to elicit a lung-localized/homing memory T cell population for further accelerated lung T cell recruitment and Mtb control. Methods CB6F1 mice vaccinated with H56 (Ag85B-ESAT6-Rv2660) adjuvanted with CAF01 (Th1/Th17-skewing liposomes) either 3× s.c., or 2× s.c. + i.n. boost(s) were rested to memory phase and then challenged with aerosol Mtb. Tissue-localized Mtb-specific T cells were enumerated by flow cytometry, and bacterial CFU determined. In vivo intravascular staining identified lung-localized from vasculature-localized T cells. Results Prime-boost with H56 resulted in a 10-fold increase in recruited/expanded Mtb-specific T cells in the lung parenchyma early after Mtb challenge over s.c.-only vaccination and a reduction in lung CFU at peak infection. However, no CFU difference was observed the chronic stage. Inhibition of S1P1 did not enhance the early protective effect, suggesting against local Trm as driving protection. Overall, the limited increased protection despite significant lung T cell enhancement suggests a constrained ability of vaccine T cells to control early Mtb replication.
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Islam, M. M. « Potentiality of native Trichoderma harzianum in controlling damping off and foot rot of chilli and its viability in different storage conditions ». Pakistan Journal of Agricultural Sciences 59, no 01 (1 janvier 2022) : 29–34. http://dx.doi.org/10.21162/pakjas/21.1558.
Texte intégralRésumé :
The potentiality of native Trichoderma harzianum against damping off and foot rot of chilli caused by Sclerotium rolfsii and Fusarium oxysporum, respectively as well as its shelf life as formulated product in different storage conditions was evaluated. In dual culture, native T. harzianum inhibited the mycelial growth of S. rolfsii by 60.56% and F. oxysporum by 81.67% over control. In green house conditions, soil received both formulated T. harzianum (20 g/kg of soil) and T. harzianum suspension (2.45×107 CFU/mL) resulted reduced severity of damping off by 81.80% and foot rot by 84.61% over control; however, the combined application of both the treatments was also resulted the highest seed germination (70.28%). The formulated T. harzianum (@ 50 g/polyethylene bags) was stored in table top, wooden shelve, tin container, wooden locker at room temperature, and in refrigerator at 4 °C. The spore density was constantly increased from the initial amount (22×106 CFU/g) in all the treatments and reached the peak after 45 days in wooden locker (73×106 CFU/g) and tin container (66×106 CFU/g) where, it reached the peak at 60 days in table top (126.3×106 CFU/g), wooden shelve (109×106 CFU/g) and refrigerator (89.67×106 CFU/g). However, the maximum population density (126.3×106 CFU/g) was observed in table top storage at 60 days which also remained highest even after 120 days (51.67×106 CFU/g) in comparison to other treatments. The findings of the study revealed that the native T. harzianum can be exploited as an effective bio-control agent for the successful disease control program in chilli as it showed extended shelf life in common storage facility.
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Babadoost, M., et D. E. Mathre. « A Method for Extraction and Enumeration of Teliospores of Tilletia indica, T. controversa, and T. barclayana in Soil ». Plant Disease 82, no 12 (décembre 1998) : 1357–61. http://dx.doi.org/10.1094/pdis.1998.82.12.1357.
Texte intégralRésumé :
A sucrose-centrifugation method was developed to extract teliospores of Tilletia indica, T. con-troversa, and T. barclayana from soil. Six soil types were artificially infested with teliospores of each of the three fungi separately to produce 102, 103, 104, or 105 teliospores per 10 g. Each 10 g of infested soil was suspended in 200 ml of water with one drop of Tween 20 and shaken for 30 s. The soil suspension was first passed through a 117-μm sieve and then through a 53-μm mesh filter, and the filtrate was collected. The filtrate was then passed through a 20-μm mesh filter, and materials caught on the mesh were washed into two 50-ml centrifuge tubes and spun for 3 min (1,200 × g). The pellet was suspended in 1.6 M sucrose solution and centrifuged for 40 s (200 × g). The supernatant was passed through a 20-μm mesh filter. The materials caught on the 20-μm mesh were collected, and the number of teliospores was determined. This procedure was initially used to extract teliospores of T. indica in soil. For extraction of teliospores of T. contro-versa and T. barclayana, 1.0 M and 1.3 M sucrose solutions, respectively, were used, and the 20-μm mesh was replaced with a 13-μm mesh filter. Teliospores of T. indica, T. controversa, T. barclayana, and T. indica-like fungus on rye grass were successfully extracted from naturally infested soils. The relationships between number of teliospores recovered from the soil and number of teliospores incorporated into the soil were Ŷ= -0.60 + 1.28(X) - 0.04(X2),Ŷ = -1.25 + 1.56(X) - 0.07(X 2), and Ŷ = -0.71 + 1.33( X) - 0.04(X2) for T. indica, T. controversa, and T. barclayana, respectively, where Ŷ = log10 of the number of teliospores recovered from soil and X = log10 of the actual number of teliospores in soil.
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Levitt, L., TJ Kipps, EG Engleman et PL Greenberg. « Human bone marrow and peripheral blood T lymphocyte depletion : efficacy and effects of both T cells and monocytes on growth of hematopoietic progenitors ». Blood 65, no 3 (1 mars 1985) : 663–79. http://dx.doi.org/10.1182/blood.v65.3.663.663.
Texte intégralRésumé :
Abstract The efficacy of four separate methods of human bone marrow T lymphocyte depletion was assessed, and the effect of T cells and monocytes on in vitro growth of marrow (CFU-GEMM, BFU-E, and CFU-GM) and peripheral blood (BFU-E) hematopoietic progenitors was determined. Extent of T cell depletion was assessed by multiparameter fluorescent cell sorter (FACS) analysis and by functional studies. Cells staining positively by FACS analysis for one or more of three separate fluorescent pan-T cell monoclonal antibodies (MCAbs) comprised 8.4% to 9.5% of control marrow mononuclear cells (MNCs). T cells constituted 3.2% to 5.1% of marrow following single, sequential, or combination treatment with two different pan-T cell MCAbs (Leu 1 and TM1) plus complement, 1.5% to 2.2% of marrow following solid-phase immunoabsorption (“panning”), 0.2% of marrow after sheep cell rosetting, and only 0.05% of marrow after FACS selective cell sorting and gated separation. T cells made up 59% to 73% of control peripheral blood MNCs and 0.8% to 2.8% of peripheral MNCs following sheep cell rosetting plus treatment with Leu 1 MCAb and complement. Mitogen (PHA, Con A) and allogeneic MLC-induced blastogenic responses (stimulation indices, experimental/control or E/C) revealed a concordant decrement in marrow T cell function after MCAb plus complement (E/C of 3.9 to 9.0), after panning (E/C of 1.6 to 3.5) and after sheep cell rosetting (E/C of 0.7 to 1.3), compared with control marrow (E/C of 5.3 to 15.7). After T cell depletion, marrow BFU-E growth was 95% to 120% of control, CFU-GM growth was 90% to 108% of control, and CFU-GEMM growth was 89% to 111% of control. Marrow T cell and/or monocyte depletion did not alter erythropoietin-dependent BFU-E growth in the absence of Mo-conditioned medium (81% to 95% of control), and the addition of as many as 50 to 100 X 10(3) purified marrow monocytes or T cells to 10(5) autologous nonadherent T cell-depleted marrow target cells had a negligible (P greater than .1) effect on marrow BFU-E growth in vitro. Peripheral blood (PB) BFU-E/10(5) T- depleted target cells were 106% +/- 19% of expected; PB BFU-E growth was significantly diminished after monocyte depletion alone (7% +/- 6% of expected) or after monocyte plus T cell depletion (8% +/- 4% of expected).(ABSTRACT TRUNCATED AT 400 WORDS)
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Levitt, L., TJ Kipps, EG Engleman et PL Greenberg. « Human bone marrow and peripheral blood T lymphocyte depletion : efficacy and effects of both T cells and monocytes on growth of hematopoietic progenitors ». Blood 65, no 3 (1 mars 1985) : 663–79. http://dx.doi.org/10.1182/blood.v65.3.663.bloodjournal653663.
Texte intégralRésumé :
The efficacy of four separate methods of human bone marrow T lymphocyte depletion was assessed, and the effect of T cells and monocytes on in vitro growth of marrow (CFU-GEMM, BFU-E, and CFU-GM) and peripheral blood (BFU-E) hematopoietic progenitors was determined. Extent of T cell depletion was assessed by multiparameter fluorescent cell sorter (FACS) analysis and by functional studies. Cells staining positively by FACS analysis for one or more of three separate fluorescent pan-T cell monoclonal antibodies (MCAbs) comprised 8.4% to 9.5% of control marrow mononuclear cells (MNCs). T cells constituted 3.2% to 5.1% of marrow following single, sequential, or combination treatment with two different pan-T cell MCAbs (Leu 1 and TM1) plus complement, 1.5% to 2.2% of marrow following solid-phase immunoabsorption (“panning”), 0.2% of marrow after sheep cell rosetting, and only 0.05% of marrow after FACS selective cell sorting and gated separation. T cells made up 59% to 73% of control peripheral blood MNCs and 0.8% to 2.8% of peripheral MNCs following sheep cell rosetting plus treatment with Leu 1 MCAb and complement. Mitogen (PHA, Con A) and allogeneic MLC-induced blastogenic responses (stimulation indices, experimental/control or E/C) revealed a concordant decrement in marrow T cell function after MCAb plus complement (E/C of 3.9 to 9.0), after panning (E/C of 1.6 to 3.5) and after sheep cell rosetting (E/C of 0.7 to 1.3), compared with control marrow (E/C of 5.3 to 15.7). After T cell depletion, marrow BFU-E growth was 95% to 120% of control, CFU-GM growth was 90% to 108% of control, and CFU-GEMM growth was 89% to 111% of control. Marrow T cell and/or monocyte depletion did not alter erythropoietin-dependent BFU-E growth in the absence of Mo-conditioned medium (81% to 95% of control), and the addition of as many as 50 to 100 X 10(3) purified marrow monocytes or T cells to 10(5) autologous nonadherent T cell-depleted marrow target cells had a negligible (P greater than .1) effect on marrow BFU-E growth in vitro. Peripheral blood (PB) BFU-E/10(5) T- depleted target cells were 106% +/- 19% of expected; PB BFU-E growth was significantly diminished after monocyte depletion alone (7% +/- 6% of expected) or after monocyte plus T cell depletion (8% +/- 4% of expected).(ABSTRACT TRUNCATED AT 400 WORDS)
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Abaturov, O. E., et A. O. Tovarnytska. « Transcription factor T-bet is a diagnostic marker for necrotizing enterocolitis development in premature newborns ». Modern pediatrics. Ukraine, no 8(128) (28 décembre 2022) : 6–11. http://dx.doi.org/10.15574/sp.2022.128.6.
Texte intégralRésumé :
Necrotizing enterocolitis (NEC) is a severe gastrointestinal disease that mainly affects preterm infants. The non-specificity of the clinical picture and the high percentage of complications determine the need for early diagnosis and prevention of the disease. Purpose - to establish a relationship between the T-bet expression level in the cells of the buccal mucosa, the development of HEC and the method of feeding in premature newborn infants. Materials and methods. We examined 74 newborns, who were treated in neonatal departments. We selected three groups for comparison: the Group 1 consisted of 32 newborns up to 37 weeks of gestation on breastfeeding (BF); the Group 2 - 30 preterm newborns on artificial feeding (AF); the control group - 12 full-term newborns on BF. We determined the expression level of T-bet transcription gene factor in the cells of the buccal mucosa scrapings of the newborns. Thereafter, we have calculated the T-bet normalized expression levels in the cells of the mucous membranes of infants of the Group 1 and the Group 2 in relation to the control group, where the expression level of the specified factors was taken as 1 c.u. Results. The gestational age median of the Group 1 children was 33 (31; 34) weeks; the Group 2 - 32.5 (32; 35) weeks; and it was comparatively higher versus in the Group 1 (p<0.001) - 40 (39; 41) weeks. NEC has significantly more often developed in premature children on AF than on BF (p<0.05) - 30.0±8.4% versus 9.4±5.2%. The T-bet expression in the cells of the buccal mucosa scrapings was higher in preterm children, and the highest level was in the Group 2 - 2.4 (1.98; 3.84) c.u. versus 1.48 (1.13; 2.22) c.u. in the Group 1. We have proved a direct relationship between AF and T-bet level (r=0.370; p<0.004). Pairwise comparison revealed a higher level of T-bet in children with NEC (p=0.002): 2.36 (1.94; 3.17) c.u versus 1.74 (1.01; 3.27) c.u. without signs of NEC. We found a direct correlation between the T-bet level and the development of HEC (r=0,271; p=0.021). Conclusions. The T-bet expression level in the cells of the buccal mucosa increases in newborns with the appearance of the clinical signs of NEC. The level of T-bet expression in the buccal mucosa cells can be a marker of the inflammatory process development in intestine of infants. The expression of T-bet in the buccal mucosa cells of newborns with clinical signs of NEC is higher than in full-term newborns that reflects the tendency to NEC development. We have demonstrated that the T-bet expression level is higher in preterm neonates on AF. Therefore, AF can be considered as a risk factor for the development of NEC in preterm infants. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of all participating institutions. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.
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Quinn, David I., Stephen J. Freedland, Elisabeth I. Heath, Ronald F. Tutrone, David G. McLeod, Nadeem Anwar Sheikh, Nancy N. Chang et A. Oliver Sartor. « Survival outcomes for African-American (AA) vs matched Caucasian (CAU) patients (pts) with metastatic castration-resistant prostate cancer (mCRPC) treated with sipuleucel-T (sip-T). » Journal of Clinical Oncology 35, no 6_suppl (20 février 2017) : 192. http://dx.doi.org/10.1200/jco.2017.35.6_suppl.192.
Texte intégralRésumé :
192 Background: Prostate cancer risk and mortality is higher in AA vs CAU men. In an analysis of three phase 3 mCRPC trials, AA pts appear to derive greater survival benefit with sip-T, an autologous cellular immunotherapy for asymptomatic/minimally symptomatic mCRPC. AA pts on sip-T had a 30.7-month (mo) median overall survival (OS) benefit vs control (AUA 2012 P953), in contrast to a 4.1-mo OS benefit with sip-T vs control for all pts in IMPACT (NEJM 2010;363:411). As the prior analysis did not adjust for baseline differences between races, we matched AA to CAU pts on sip-T to better assess racial differences in sip-T benefit and cumulative antigen presenting cell (APC) activation, which correlates with OS (CII 2013;62:137). Methods: Data were from phase 3 mCRPC trials that randomized pts 2:1 to sip-T or control (D9901 [NCT00005947]; D9902A [NCT01133704]; IMPACT [NCT00065442]). Thirty-three AA sip-T pts were matched with 66 CAU sip-T pts based on predicted Halabi survival. Data were evaluated by the Kaplan-Meier method (event rates) and ANOVA (APC activation). Results: Median follow-up was 23.2 mos. AA pts on sip-T had a 20.6-mo longer median OS (45.3 mos; 95% CI 23.4–NE) vs CAU pts (24.7 mos; 95% CI 18.1–29.4) (HR = 0.49; 95% CI 0.26–0.91; p = 0.02). Median event-free survival (time to death or anticancer intervention [ACI]) was 10.7 mos for AA pts (95% CI 8.5–21.5) vs 8.7 mos for CAU pts (95% CI 6.6–11.5) (HR = 0.74; 95% CI 0.47–1.18; p = 0.20). Median time to next ACI was 23.5 mos in AA pts (95% CI 9.4–NE) vs 16.3 mos in CAU pts (95% CI 9.7–25.6) (HR = 0.78; 95% CI 0.42–1.43; p = 0.42). In AA pts, median APC activation was higher with sip-T infusion 1 vs CAU pts (7.0 vs 5.5, p = 0.004). Median cumulative APC activation over the three infusions was 27.7 (22.6–42.4, AA) vs 25.7 (21.5–33.9, CAU) (p = 0.083). Conclusions: Prior studies found sip-T provides OS benefit to both AA and CAU mCRPC pts. Herein, for men treated with sip-T, AA had longer survival, suggesting sip-T may provide greater OS benefit in AA. The basis for this may be biologic (greater APC activation). Further studies with larger sample sizes are needed to confirm if AA pts derive greater OS benefit from sip-T. Clinical trial information: NCT00005947; NCT01133704; NCT00065442.
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Qi Junfeng, 祁俊峰, 牛振 Niu Zhen, 张冬云 Zhang Dongyun et 左铁钏 Zuo Tiechuan. « Control of Shaping of Welds for CO2 Laser Welding Shipbuilding T-Section Aluminum Alloy ». Chinese Journal of Lasers 35, no 2 (2008) : 297–302. http://dx.doi.org/10.3788/cjl20083502.0297.
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Raj, C. Retna, et R. Ramaraj. « Emission of Thioflavin T and its Off-On Control in Polymer Membranes‡¶ ». Photochemistry and Photobiology 74, no 6 (1 mai 2007) : 752–59. http://dx.doi.org/10.1562/0031-8655(2001)0740752eottai2.0.co2.
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Jiang, Shang, Zhi Lan Lin, Cui Wang, Jin Long Jiang et Jian Ping Hou. « Zoning Compliance Control Method Applied in Regional Planning of the Marine Environment Capacity Calculation ». Advanced Materials Research 726-731 (août 2013) : 1199–203. http://dx.doi.org/10.4028/www.scientific.net/amr.726-731.1199.
Texte intégralRésumé :
This paper uses control points quality compliance method to calculate the COD, inorganic nitrogen, active phosphate marine environment capacity of Luoyuan Bay. Control point quality compliance method to calculate the environmental capacity of the location of the emission of pollutants, the distribution density and control point location is closely related to its value with the changes in the number of outfall and sewage position changes and change. Conditions of existing sources of pollution distribution, COD, inorganic nitrogen (Din), active phosphate environment capacity (PO4-P) for 602207t/a2576 t/a724 t/a.
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Śliwińska, Anna, Aleksandra Strugała-Wilczek, Piotr Krawczyk, Agnieszka Leśniak, Tomasz Urych, Jarosław Chećko et Krzysztof Stańczyk. « Carbon Capture Utilisation and Storage Technology Development in a Region with High CO2 Emissions and Low Storage Potential—A Case Study of Upper Silesia in Poland ». Energies 15, no 12 (20 juin 2022) : 4495. http://dx.doi.org/10.3390/en15124495.
Texte intégralRésumé :
The region of Upper Silesia, located in southern Poland, is characterised by very high emissions of carbon dioxide into the air—the annual emission exceeds 33 Mt CO2 and the emission ‘per capita’ is 7.2 t/y in comparison to the EU average emission per capita 6.4 t/y and 8.4 t/y for Poland in 2019. Although in the region there are over 100 carbon dioxide emitters covered by the EU ETS, over 90% of emissions come from approximately 15 large hard coal power plants and from the coke and metallurgical complex. The CCUS scenario for Upper Silesia, which encompasses emitters, capture plants, transport routes, as well as utilisation and storage sites until 2050, was developed. The baseline scenario assumes capture of carbon dioxide in seven installations, use in two methanol plants and transport and injection into two deep saline aquifers (DSA). The share of captured CO2 from flue gas was assumed at the level of 0.25–0.9, depending mainly on the limited capacity of storage. To recognise the views of society on development of the CCUS technologies in Upper Silesia, thirteen interviews with different types of stakeholders (industry, research and education, policy makers) were conducted. The respondents evaluated CCU much better than CCS. The techno-economic assessment of CCUS carried out on a scenario basis showed that the economic outcome of the scenario with CCUS is EUR 3807.19 million more favourable compared to the scenario without CO2 capture and storage.
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Belly T. Dionio, Larry V. Aceres, Darryl Kynth B. Pontillo et Mary Amor G. Figueroa. « Delivery System for Enhanced Efficacy of Trichoderma harzianum against Fusarium Wilt of Lakatan Banana ». Journal of Science, Engineering and Technology (JSET) 8, no 1 (5 décembre 2020) : 27–40. http://dx.doi.org/10.61569/mvwgt618.
Texte intégralRésumé :
Biological control is a potential approach in the management of Fusarium wilt (Foc) of banana. To be effective, however, biological disease control depends not only on suitable biocontrol organisms but also on methods and strategies of introducing and maintaining these organisms in association with plants. This paper presents the influence of delivery systems on the efficacy of Trichoderma harzianum for Fusarium wilt control in ‘Lakatan’ banana. T. harzianum was applied prior to transplanting and during transplanting in 30 cm X 30 cm polypropylene bags with follow-up application of T. harzianum in the forms of conidial suspension every two weeks or in substrate at monthly intervals. Negative and positive controls were provided. Among the delivery systems, disease incidence and severity and Foc colony-forming units (CFU) per gram of substrate were significantly (p <0.001) low in meriplants grown in T. harzianum-inoculated planting medium with basal and monthly follow-up application of T. harzianum in substrate or composted coco coir dust (67% incidence; disease severity index (DSI)=2.0; cfu/g medium=2,050). The effects were comparable to incorporation of T. harzianum in the potting medium of three-week-old plants with basal application of T. harzianum in substrate at transplanting and follow-up application of T. harzianum suspension every two weeks (60% incidence; DSI=2.1; cfu/g medium=5,790). Likewise, comparable results were obtained with pre-treated three-week-old potted plants with basal application of T. harzianum in substrate at transplanting but no follow-up application of T. harzianum (67% incidence; DSI=2.1; cfu/g medium=6,240). Costs incurred per plant in the application of T. harzianum were P2.41, P3.03, and P1.28, respectively.
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Kanae, M., K. Hisaichi, S. Jaswant et D. Osamu. « 214 EFFECT OF LONG-DISTANCE TRANSPORTATION OF OVARIES ON THE DEVELOPMENT OF IN VITRO-MATURED - IN VITRO-FERTILIZED - IN VITRO-CULTURED BOVINE EMBRYOS ». Reproduction, Fertility and Development 23, no 1 (2011) : 206. http://dx.doi.org/10.1071/rdv23n1ab214.
Texte intégralRésumé :
Keeping ovaries for a long time before oocyte recovery affects the maturation rate and blastocyst rate (BL rate), but a change in the temperature of the ovary at the time of transportation does not affect the BL rate (Ribeiro et al. 2008 Anim. Reprod. Sci. 108, 171–179). The objective of this study was to investigate the effect of long-distance transportation of ovaries on the in vitro development of bovine oocytes into blastocysts. Ovaries in the transportation group (Group T) were collected from a slaughterhouse, placed inside a Thermos flask, and transported to the laboratory within 17 to 21 h. The Thermos flask was covered with a freezer pack in a foam polystyrene box (Nakatate et al. 2006 Reprod. Fertil. Dev. 18, 193). The temperature of the Thermos flask was measured with a temperature recorder. Cumulus–oocyte complexes (COC) were collected by aspirating 2- to 6-mm follicles from the ovaries. The COC were matured in TCM-199 supplemented with 5% calf serum and 0.02 AU mL–1 of FSH. Groups of 20 COC were incubated in 100-μL drops of IVM media at 38.5°C under an atmosphere of 5% CO2 in air for 20 h. After 18 h of gamete co-culture (3 × 106 sperm mL–1), the presumptive zygotes were cultured in CR1aa medium supplemented with 5% calf serum for 9 days at 38.5°C under an atmosphere of 5% CO2, 5% O2, and 90% N2. Embryonic development was evaluated at 48 h after IVF (total cleavage rates, CL rate) and on Days 7 to 9 (BL rate). The ovaries in the control group (Group C) were collected from a slaughterhouse different from that of the Group T ovaries and transported to the laboratory in saline (23°C) in a Thermos flask within 3 h. These ovaries were then used for the collection of COC in the same way as for Group T. Data were analysed by chi-square test. A total of 25 experiments were performed using the ovaries from Groups C and T (n = 3945 v. n = 7218). The CL rate and the BL rate for Group T were significantly lower than those for Group C (41.8 v. 62.0% and 16.1 v. 29.7%; P < 0.01). In Group T, the duration of ovary transportation did not affect the CL rate (within 19 h, between 19 and 20 h, and more than 20 h), but the BL rate was significantly decreased with an increase in transport time (18.7 v. 16.1 v. 14.8%). The temperature change in the Thermos flask during transportation ranged from 12.0 to 25.1°C; the temperature was maintained virtually constant or decreased. The CL rates of the groups that underwent a temperature (t) change (t ≤ 5.0 and 5.0 < t ≤ 10.0) were not significantly different, but the BL rate in the group with 5.0 < t ≤ 10.0 was significantly lower than that of the other group (16.7 v. 13.5%; P < 0.01). These results suggest that the long-distance transportation of ovaries affects in vitro embryo development. Moreover, a decrease in temperature during transportation may have an adverse effect on blastocyst formation.
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De Macedo, Ana Carolina Brandt. « Avaliação da qualidade de vida de trabalhadores de um setor de limpeza hospitalar submetidos à aplicação do método isostretching ». Fisioterapia Brasil 11, no 5 (9 décembre 2017) : 340. http://dx.doi.org/10.33233/fb.v11i5.1419.
Texte intégralRésumé :
Objetivo: Avaliar a qualidade de vida dos trabalhadores do setor de limpeza hospitalar submetidos à aplicação do método isostretching. Material e métodos: Foram selecionadas 14 participantes, sexo feminino, faixa etária de 30 a 50 anos de idade, trabalhadores de setor de limpeza do Hospital Geral de Curitiba, as quais foram submetidas a aplicação do método isostretching por 3 sessões semanais de 15 minutos por 4 meses. Como instrumento de avaliação foi aplicado o questionário de qualidade de vida (SF-36). Resultados: Obteve-se a melhora da qualidade de vida nessas trabalhadoras, destacando-se o domínio dor com melhora de 86%. Após a realização da análise estatística feita pelo teste t-pareado, verifi cou-se um valor de signifi cância (p < 0,05) em todos os domínios do questionário. Conclusão: O método isostretching possibilitou uma melhora da qualidade de vida nas trabalhadoras do setor de limpeza podendo ser útil como tratamento ou prevenção de doenças laborais. Palavras-chave: qualidade de vida, terapia por exercício, saúde do trabalhador, isostretching.
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Majado, Maria Juliana, María I. Macizo, Consuelo González-García, Eduardo Salido, José A. Molina, Jorge Monserrat et Alfonso Morales. « Megakaryocytic Progenitors in Long Term Cultures from MDS Patients. » Blood 106, no 11 (16 novembre 2005) : 4924. http://dx.doi.org/10.1182/blood.v106.11.4924.4924.
Texte intégralRésumé :
Abstract Introduction: bone marrow long-term cultures (LTC) have been reported to produce very poor adherent stromal layer in MDS, but there are not many studies about the behaviour of the progenitor cells in supernatant culture medium. In this study we report the results of megakaryocytic progenitors (CFU-MK) in the supernatant of LTC of patients with refractory anemia (RA). Material and method: LTCs were performed to 11 RA patients and to 13 normal multiorgan donors as control. Bone marrow red cells were sedimented with 1% methyl-cellulose and seeded for LTC in 10ml flasks (Nalge Nunc International), with Iscover medium supplemented with horse serum, foetal calf serum, hydrocortisone and carbonic air, at a final concentration of 1x106 per ml. Flasks were placed in an incubator for 8 weeks. Half culture medium volume was renovated weekly, cell counts and assays of CFU-MK (Megacult C, Stem Cell Technologies), were performed. CFU-MK colonies were separated in three groups, containing 3–20 cells, 21–50 cells and more than 50 cells. Student t-test was used for statistical comparisons. Results: Are in the following table, expressed as mean + standard desviation. After the third week no colony growth was observed in normal such as in MDS. Growth of CFU-MK colonies containing more than 50 cells was higher in basal control bone marrow, no statistical differences were found in the rest of the cultures. Results CFU-MK(3–30 Cells) CFU-MK(20–50 Cells) CFU-MK (>50 Cells) CFU-MK total w: week Control basal 15.75±15.79 3.33±5.42 4.88±7.44 22.42±26.43 RA basal 7.85±8.66 0.80±1.01 0.70±0.98 9.45±9.46 t-St ns ns 0.047 ns Control w 1 3.54±4.42 0.7±1.44 0.74±1.48 4.98±6.62 RA w 1 3.29±4.52 0.29±0.93 0.25±0.67 3.82±5.65 t-St ns ns ns ns Control w 2 0.55±1.14 0.07±0.24 0.05±0.25 0.67±1.43 RA w 2 0.18±0.32 0.00±0.00 0.04±0.13 0.21±0.38 t-St ns ns ns ns Control w 3 0.18±0.53 0.00±0.00 0.00±0.00 0.18±0.53 RA w 3 0.00±0.21 0.00±0.00 0.00±0.00 0.00±0.21 t-St ns ns Discussion: No important difference was found in LTC supernatant CFU-MK in our RA patients, and this supports the idea that the stromal damage is more important, in the pathophysyology of MDS, than that of the stem cells.
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Azzolin, M., E. Zanetti, R. Conte et D. Del Col. « Control of CO2 evaporation in an integrated photovoltaic module : experiments and modelling ». Journal of Physics : Conference Series 2685, no 1 (1 janvier 2024) : 012048. http://dx.doi.org/10.1088/1742-6596/2685/1/012048.
Texte intégralRésumé :
Abstract The use of environmentally friendly refrigerants and the improvement of the system performance are two main topics in the research on heat pump technology. Given the recent limitations imposed on the usage of high global warming potential refrigerants, there is a growing demand in the market for the adoption of natural refrigerants, like CO2. Nevertheless, CO2 heat pumps operate under transcritical conditions, which can adversely affect its efficiency. Therefore, incorporating a solar heat source into a CO2 heat pump can offer a solution to mitigate low system performance issues. In the present study, a photovoltaic module integrated with the evaporator of a CO2 heat pump is experimentally studied. The PV-T evaporator exploits solar radiation to both generate electricity in the PV cells and ensure the evaporation of CO2 in the collector’s tubes. Simultaneously, the PV conversion efficiency is improved by the cooling effect of the evaporation. The present evaporator works in dry expansion mode, thus the refrigerant flow after the expansion device is sent to the solar collectors, where it evaporates before returning to the compressor. When using a PV-T evaporator, it is necessary to prevent superheating in the evaporator to keep a uniform and efficient cooling of the PV cells. The current heat pump design prevents the occurrence of superheated vapor at the PV-T evaporator’s outlet. Beside the experimental activity a dynamic numerical model of the system has been developed and validated.
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Mangan, KF, et L. D'Alessandro. « Hypoplastic anemia in B cell chronic lymphocytic leukemia : evolution of T cell-mediated suppression of erythropoiesis in early-stage and late- stage disease ». Blood 66, no 3 (1 septembre 1985) : 533–41. http://dx.doi.org/10.1182/blood.v66.3.533.533.
Texte intégralRésumé :
Abstract To define further the role of marrow T suppressor lymphocytes in the pathogenesis of the hypoproliferative anemia in all Rai clinical stages of B cell chronic lymphocytic leukemia (CLL), marrow erythroid progenitor cell (CFU-E and BFU-E) frequency, marrow T gamma lymphocyte frequency per 1,000 nucleated marrow cells, and T cell-erythroid progenitor cell interactions were examined in 30 CLL patients and normal control subjects. As compared with control subjects, decreased numbers of CFU-E and BFU-E were found in patient marrow depleted of neoplastic B cells in all Rai stages of the disease. As a group, Rai stage III through IV patients with or without aplasia (CLL-aplasia) had significantly fewer CFU-E and BFU-E than did Rai O through II stage patients. The numbers of T gamma cells infiltrating CLL marrows were increased 3, 9, and 20 times normal in Rai O through II, Rai III through IV, and CLL-aplasia groups, respectively. Removal of T cells from marrow increased growth of CFU-E and BFU-E in all Rai O through IV patients, but the increase was significant in the CLL-aplasia group only (P less than .05). However, autologous coculture of marrow T cells or T gamma cells but not B cells with marrow B + T-depleted null cells at ratios of 0.2:1 to 1:1 suppressed CFU-E and BFU-E growth in all three patient groups. We conclude that the hypoproliferative anemia occurring in the course of B cell CLL is due to gradual accumulation in the marrow of T gamma lymphocytes which suppress erythroid progenitor cell growth. T gamma cell suppression of erythropoiesis and marrow T gamma cell expansion is detectable in the earliest Rai stages of the disease.
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Mangan, KF, et L. D'Alessandro. « Hypoplastic anemia in B cell chronic lymphocytic leukemia : evolution of T cell-mediated suppression of erythropoiesis in early-stage and late- stage disease ». Blood 66, no 3 (1 septembre 1985) : 533–41. http://dx.doi.org/10.1182/blood.v66.3.533.bloodjournal663533.
Texte intégralRésumé :
To define further the role of marrow T suppressor lymphocytes in the pathogenesis of the hypoproliferative anemia in all Rai clinical stages of B cell chronic lymphocytic leukemia (CLL), marrow erythroid progenitor cell (CFU-E and BFU-E) frequency, marrow T gamma lymphocyte frequency per 1,000 nucleated marrow cells, and T cell-erythroid progenitor cell interactions were examined in 30 CLL patients and normal control subjects. As compared with control subjects, decreased numbers of CFU-E and BFU-E were found in patient marrow depleted of neoplastic B cells in all Rai stages of the disease. As a group, Rai stage III through IV patients with or without aplasia (CLL-aplasia) had significantly fewer CFU-E and BFU-E than did Rai O through II stage patients. The numbers of T gamma cells infiltrating CLL marrows were increased 3, 9, and 20 times normal in Rai O through II, Rai III through IV, and CLL-aplasia groups, respectively. Removal of T cells from marrow increased growth of CFU-E and BFU-E in all Rai O through IV patients, but the increase was significant in the CLL-aplasia group only (P less than .05). However, autologous coculture of marrow T cells or T gamma cells but not B cells with marrow B + T-depleted null cells at ratios of 0.2:1 to 1:1 suppressed CFU-E and BFU-E growth in all three patient groups. We conclude that the hypoproliferative anemia occurring in the course of B cell CLL is due to gradual accumulation in the marrow of T gamma lymphocytes which suppress erythroid progenitor cell growth. T gamma cell suppression of erythropoiesis and marrow T gamma cell expansion is detectable in the earliest Rai stages of the disease.
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Navarrete, Wilfredo, Pilar Durruty, Eileen Andrade, Rodrigo Orellana, María Georgina Ortiz et María Angélica Budini. « Metabolic control in type 2 diabetic patients treated with empagliflozin : A case series ». Medwave 24, no 03 (17 avril 2024) : e2758-e2758. http://dx.doi.org/10.5867/medwave.2024.03.2758.
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Background Type 2 diabetes mellitus is a highly prevalent disease and is associated with increased morbidity and mortality. Due to the low percentage of adequate glycemic control, new strategies for the treatment of type 2 diabetes mellitus have been sought, including sodium-glucose cotransporter type 2 inhibitorss. Objective To describe the evolution of patients with type 2 diabetes mellitus with insulin requirements treated with empagliflozin at the Peñaflor Hospital. The primary objective was to evaluate the efficacy of the medication regarding glycosylated hemoglobin A1c (HbA1c). The secondary objectives were: 1) achievement of HbA1c equal to or less than 7.5% according to survival analysis. 2) Change in glomerular filtration rate and urinary albumin excretion post treatment. Methods Review of clinical records of all patients treated with empagliflozin from November 2019 to June 2023. Average follow-up of 19 (16.3 to 40) months. To compare HbA1c values according to follow-up ranges, the paired T test or Wilcoxon test was used. Results We included 58 patients, 15 men and 43 women (74.1%), with an average age of 58.5 ± 9.2 years, ranging from 35 to 75 years. Baseline HbA1c of 10.3 ± 1.6% and 8.98% ± 2.2 in a follow-up of 18 to 24 months post-treatment, resulted in a decrease of 1.27% (p = 0.002; confidence interval 95%: 0.5 to 2.03). The most common adverse effect was urinary tract infection. Conclusions Patients with type 2 diabetes mellitus with insulin requirements treated with empagliflozin at the Peñaflor Hospital achieved better glycemic control with few adverse effects.
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Sari, Desi Ambar, et Rusnilawati Rusnilawati. « Pengaruh Penerapan Model PBL dengan Media Takalintar terhadap Hasil Belajar Matematika Siswa ». Jurnal Obsesi : Jurnal Pendidikan Anak Usia Dini 6, no 6 (4 octobre 2022) : 6017–26. http://dx.doi.org/10.31004/obsesi.v6i6.3143.
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Rendahnya keterampilan dalam memecahkan masalah dan terbatasnya media yang guru gunakan dalam menyampaikan materi, sangat berdampak pada hasil belajar matematika. Tujuan penelitian ini untuk menganalisis tentang pengaruh penerapan Problem Based Learning dengan media Takalintar terhadap hasil belajar pada materi matematika. Jenis penelitian ini adalah kuantitatif dengan metode Nonequivalent Contro Group Design. Dalam penelitian ini peneliti menggunakan 2 kelas yaitu kelas eksperimen yang diberikan perlakuan dan kelas kontrol tanpa perlakuan. Populasi dalam penelitian ini adalah seluruh siswa kelas III. Sampel yang digunakan pada penelitian ini siswa kelas IIIA dan IIIB berjumlah 44 siswa. Teknik pengumpulan data dengan tes, observasi dan dokumentasi. Instrumen yang digunakan ialah soal tes yang berbentuk soal uraian untuk membandingkan hasil belajar. Analisis data menggunakan uji normalitas dan uji hipotesis T-test dengan bantuan SPSS 22. Hasil pengujian menunjukkan kelas eksperimen pretest dan postest memiliki nilai sig kurang dari a, (0,000 kurang dari 0,05) yang artinya H0 ditolak dan Ha diterima.
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Sankari, Abdulghani, Amy T. Bascom, Susmita Chowdhuri et M. Safwan Badr. « Tetraplegia is a risk factor for central sleep apnea ». Journal of Applied Physiology 116, no 3 (1 février 2014) : 345–53. http://dx.doi.org/10.1152/japplphysiol.00731.2013.
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Sleep-disordered breathing (SDB) is highly prevalent in patients with spinal cord injury (SCI); the exact mechanism(s) or the predictors of disease are unknown. We hypothesized that patients with cervical SCI (C-SCI) are more susceptible to central apnea than patients with thoracic SCI (T-SCI) or able-bodied controls. Sixteen patients with chronic SCI, level T6 or above (8 C-SCI, 8 T-SCI; age 42.5 ± 15.5 years; body mass index 25.9 ± 4.9 kg/m2) and 16 matched controls were studied. The hypocapnic apneic threshold and CO2 reserve were determined using noninvasive ventilation. For participants with spontaneous central apnea, CO2 was administered until central apnea was abolished, and CO2 reserve was measured as the difference in end-tidal CO2 (PetCO2) before and after. Steady-state plant gain (PG) was calculated from PetCO2 and VE ratio during stable sleep. Controller gain (CG) was defined as the ratio of change in VE between control and hypopnea or apnea to the ΔPetCO2. Central SDB was more common in C-SCI than T-SCI (63% vs. 13%, respectively; P < 0.05). Mean CO2 reserve for all participants was narrower in C-SCI than in T-SCI or control group (−0.4 ± 2.9 vs.−2.9 ± 3.3 vs. −3.0 ± 1.2 l·min−1·mmHg−1, respectively; P < 0.05). PG was higher in C-SCI than in T-SCI or control groups (10.5 ± 2.4 vs. 5.9 ± 2.4 vs. 6.3 ± 1.6 mmHg·l−1·min−1, respectively; P < 0.05) and CG was not significantly different. The CO2 reserve was an independent predictor of apnea-hypopnea index. In conclusion, C-SCI had higher rates of central SDB, indicating that tetraplegia is a risk factor for central sleep apnea. Sleep-related hypoventilation may play a significant role in the mechanism of SDB in higher SCI levels.
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Herbert, D. A. « Corn Earworm Control in 'Nc T Peanut in Virginia, 1990 ». Insecticide and Acaricide Tests 16, no 1 (1 janvier 1991) : 209. http://dx.doi.org/10.1093/iat/16.1.209a.
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Abstract Efficacy of selected insecticides was evaluated against CEW in cv. NC 7 virginia-type peanut in Suffolk, Va. Peanuts were planted 2 May using a 36-inch row spacing. Temik 15 G at 1 lb (AI)/acre was applied in-furrow at planting. Test treatments were applied on 20 Aug using a CO2-pressurized backpack sprayer calibrated to deliver 14.5 gal formulation/acre at 50 psi through three D2-13 disc-core cone spray nozzles per row, 1 over the top and 1 on each side. A randomized complete block experimental design was used with 4 replicates; plots were 4 rows by 30 ft. Treatments were evaluated at 2, 7 and 14 DAT by comparing numbers of small, medium and large-sized CEW larvae, corresponding to lst-2nd, 3rd-4th and 5th-6th instar groupings, respectively, per 3 row-ft. Pretreatment means were determined from four 3 row-ft samples per replicate taken before treatments were applied on 20 Aug. Yield was determined by digging, combining (28 Sep), drying and weighing peanuts from the two center rows of each plot.
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Schneider, M. « La chimiothérapie d’induction a-t-elle encore une place dans le traitement des carcinomes épidermoïdes de la tête et du cou ? » Oncologie 7, no 4 (juin 2005) : 271–75. http://dx.doi.org/10.1007/s10269-005-0212-2.
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