Bibliographies thématiques / Chemosensory gene

Littérature scientifique sur le sujet « Chemosensory gene »

Auteur : Grafiati
Publié le 14 septembre 2024

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Articles de revues sur le sujet "Chemosensory gene"

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Vizueta, Joel, Paula Escuer, Cristina Frías-López, Sara Guirao-Rico, Lars Hering, Georg Mayer, Julio Rozas et Alejandro Sánchez-Gracia. « Evolutionary History of Major Chemosensory Gene Families across Panarthropoda ». Molecular Biology and Evolution 37, no 12 (4 août 2020) : 3601–15. http://dx.doi.org/10.1093/molbev/msaa197.

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Abstract Chemosensory perception is a fundamental biological process of particular relevance in basic and applied arthropod research. However, apart from insects, there is little knowledge of specific molecules involved in this system, which is restricted to a few taxa with uneven phylogenetic sampling across lineages. From an evolutionary perspective, onychophorans (velvet worms) and tardigrades (water bears) are of special interest since they represent the closest living relatives of arthropods, altogether comprising the Panarthropoda. To get insights into the evolutionary origin and diversification of the chemosensory gene repertoire in panarthropods, we sequenced the antenna- and head-specific transcriptomes of the velvet worm Euperipatoides rowelli and analyzed members of all major chemosensory families in representative genomes of onychophorans, tardigrades, and arthropods. Our results suggest that the NPC2 gene family was the only family encoding soluble proteins in the panarthropod ancestor and that onychophorans might have lost many arthropod-like chemoreceptors, including the highly conserved IR25a receptor of protostomes. On the other hand, the eutardigrade genomes lack genes encoding the DEG-ENaC and CD36-sensory neuron membrane proteins, the chemosensory members of which have been retained in arthropods; these losses might be related to lineage-specific adaptive strategies of tardigrades to survive extreme environmental conditions. Although the results of this study need to be further substantiated by an increased taxon sampling, our findings shed light on the diversification of chemosensory gene families in Panarthropoda and contribute to a better understanding of the evolution of animal chemical senses.
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Xu, Ji-Wei, Xiu-Yun Zhu, Qiu-Jie Chao, Yong-Jie Zhang, Yu-Xia Yang, Ran-Ran Wang, Yu Zhang et al. « Chemosensory Gene Families in the Oligophagous Pear Pest Cacopsylla chinensis (Hemiptera : Psyllidae) ». Insects 10, no 6 (17 juin 2019) : 175. http://dx.doi.org/10.3390/insects10060175.

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Chemosensory systems play an important role in insect behavior, and some key associated genes have potential as novel targets for pest control. Cacopsylla chinensis is an oligophagous pest and has become one of the main pests of pear trees, but little is known about the molecular-level means by which it locates its hosts. In this study, we assembled the head transcriptome of C. chinensis using Illumina sequencing, and 63,052 Unigenes were identified. A total of 36 candidate chemosensory genes were identified, including five different families: 12 odorant binding proteins (OBPs), 11 chemosensory proteins (CSPs), 7 odorant receptors (ORs), 4 ionotropic receptors (IRs), and 2 gustatory receptors (GRs). The number of chemosensory gene families is consistent with that found in other Hemipteran species, indicating that our approach successfully obtained the chemosensory genes of C. chinensis. The tissue expression of all genes using quantitative real-time PCR (qRT-PCR) found that some genes displayed male head, female head, or nymph-biased specific/expression. Our results enrich the gene inventory of C. chinensis and provide valuable resources for the analysis of the functions of some key genes. This will help in developing molecular targets for disrupting feeding behavior in C. chinensis.
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Segura-León, Obdulia L., Brenda Torres-Huerta, Alan Rubén Estrada-Pérez, Juan Cibrián-Tovar, Fidel de la Cruz Hernandez-Hernandez, José Luis Cruz-Jaramillo, José Salvador Meza-Hernández et Fabian Sánchez-Galicia. « Identification of Candidate Chemosensory Gene Families by Head Transcriptomes Analysis in the Mexican Fruit Fly, Anastrepha ludens Loew (Diptera : Tephritidae) ». International Journal of Molecular Sciences 23, no 18 (11 septembre 2022) : 10531. http://dx.doi.org/10.3390/ijms231810531.

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Insect chemosensory systems, such as smell and taste, are mediated by chemosensory receptor and non-receptor protein families. In the last decade, many studies have focused on discovering these families in Tephritidae species of agricultural importance. However, to date, there is no information on the Mexican fruit fly Anastrepha ludens Loew, a priority pest of quarantine importance in Mexico and other countries. This work represents the first effort to identify, classify and characterize the six chemosensory gene families by analyzing two head transcriptomes of sexually immature and mature adults of A. ludens from laboratory-reared and wild populations, respectively. We identified 120 chemosensory genes encoding 31 Odorant-Binding Proteins (OBPs), 5 Chemosensory Proteins (CSPs), 2 Sensory Neuron Membrane Proteins (SNMPs), 42 Odorant Receptors (ORs), 17 Ionotropic Receptors (IRs), and 23 Gustatory Receptors (GRs). The 120 described chemosensory proteins of the Mexican fruit fly significantly contribute to the genetic databases of insects, particularly dipterans. Except for some OBPs, this work reports for the first time the repertoire of olfactory proteins for one species of the genus Anastrepha, which provides a further basis for studying the olfactory system in the family Tephritidae, one of the most important for its economic and social impact worldwide.
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Rondoni, Gabriele, Alessandro Roman, Camille Meslin, Nicolas Montagné, Eric Conti et Emmanuelle Jacquin-Joly. « Antennal Transcriptome Analysis and Identification of Candidate Chemosensory Genes of the Harlequin Ladybird Beetle, Harmonia axyridis (Pallas) (Coleoptera : Coccinellidae) ». Insects 12, no 3 (2 mars 2021) : 209. http://dx.doi.org/10.3390/insects12030209.

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In predatory ladybirds (Coleoptera: Coccinellidae), antennae are important for chemosensory reception used during food and mate location, and for finding a suitable oviposition habitat. Based on NextSeq 550 Illumina sequencing, we assembled the antennal transcriptome of mated Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) males and females and described the first chemosensory gene repertoire expressed in this species. We annotated candidate chemosensory sequences encoding 26 odorant receptors (including the coreceptor, Orco), 17 gustatory receptors, 27 ionotropic receptors, 31 odorant-binding proteins, 12 chemosensory proteins, and 4 sensory neuron membrane proteins. Maximum-likelihood phylogenetic analyses allowed to assign candidate H. axyridis chemosensory genes to previously described groups in each of these families. Differential expression analysis between males and females revealed low variability between sexes, possibly reflecting the known absence of relevant sexual dimorphism in the structure of the antennae and in the distribution and abundance of the sensilla. However, we revealed significant differences in expression of three chemosensory genes, namely two male-biased odorant-binding proteins and one male-biased odorant receptor, suggesting their possible involvement in pheromone detection. Our data pave the way for improving the understanding of the molecular basis of chemosensory reception in Coccinellidae.
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Braun, Thomas, Brigitte Mack et Matthias F. Kramer. « Solitary chemosensory cells in the respiratory and vomeronasal epithelium of the human nose : a pilot study ». Rhinology journal 49, no 5 (1 décembre 2011) : 507–12. http://dx.doi.org/10.4193/rhino11.121.

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Background: Recently, solitary chemosensory cells have been described in the respiratory and vomeronasal epithelium of the rodent nose. Expressing G-protein coupled receptors for sweet, umami and bitter taste transduction, these cells are thought to mediate trigeminal reflexes upon stimulation with chemical irritants. The present study analyzes human nasal mucosa for the presence of solitary chemosensory cells. Methodology: In human tissue samples from respiratory mucosa and the vomeronasal organ, gene expression of taste receptors families was studied in five patients using the Affymetrix Human Gene 1.0 ST Array and immunohistochemistry with specific antibodies. Results: Immunohistochemistry revealed that solitary chemosensory cells expressing G-protein coupled receptors for sweet, umami and bitter taste transduction are present in the human nose. cDNA microarray analysis congruently showed that cells expressing bitter taste receptors accumulate in the vomeronasal organ compared to the respiratory epithelium. Conclusions: Solitary chemosensory cells expressing taste receptors are also present in the human nose. Since they are thought to mediate trigeminal reflexes, their role in the pathogenesis of nasal hyperreagibility should be elucidated in further studies.
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Braun, Thomas, Brigitte Mack et Matthias F. Kramer. « Solitary chemosensory cells in the respiratory and vomeronasal epithelium of the human nose : a pilot study ». Rhinology journal 49, no 5 (1 décembre 2011) : 507–12. http://dx.doi.org/10.4193/rhino.11.121.

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Background: Recently, solitary chemosensory cells have been described in the respiratory and vomeronasal epithelium of the rodent nose. Expressing G-protein coupled receptors for sweet, umami and bitter taste transduction, these cells are thought to mediate trigeminal reflexes upon stimulation with chemical irritants. The present study analyzes human nasal mucosa for the presence of solitary chemosensory cells. Methodology: In human tissue samples from respiratory mucosa and the vomeronasal organ, gene expression of taste receptors families was studied in five patients using the Affymetrix Human Gene 1.0 ST Array and immunohistochemistry with specific antibodies. Results: Immunohistochemistry revealed that solitary chemosensory cells expressing G-protein coupled receptors for sweet, umami and bitter taste transduction are present in the human nose. cDNA microarray analysis congruently showed that cells expressing bitter taste receptors accumulate in the vomeronasal organ compared to the respiratory epithelium. Conclusions: Solitary chemosensory cells expressing taste receptors are also present in the human nose. Since they are thought to mediate trigeminal reflexes, their role in the pathogenesis of nasal hyperreagibility should be elucidated in further studies.
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Chen, N., S. Pai, Z. Zhao, A. Mah, R. Newbury, R. C. Johnsen, Z. Altun, D. G. Moerman, D. L. Baillie et L. D. Stein. « Identification of a nematode chemosensory gene family ». Proceedings of the National Academy of Sciences 102, no 1 (23 décembre 2004) : 146–51. http://dx.doi.org/10.1073/pnas.0408307102.

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Athrey, Giridhar, Zachary R. Popkin-Hall, Willem Takken et Michel A. Slotman. « The Expression of Chemosensory Genes in Male Maxillary Palps of Anopheles coluzzii (Diptera : Culicidae) and An. quadriannulatus ». Journal of Medical Entomology 58, no 3 (12 février 2021) : 1012–20. http://dx.doi.org/10.1093/jme/tjaa290.

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Abstract Because of its importance as a malaria vector, Anopheles coluzzii’s Coetzee & Wilkerson olfactory system has been studied extensively. Among this work is a series of studies comparing the expression of chemosensory genes in olfactory organs in females and/or males of these species. These have identified species- and female-biased chemosensory gene expression patterns. However, many questions remain about the role of chemosensation in male anopheline biology. To pave the way for future work we used RNAseq to compare chemosensory gene expression in the male maxillary palps of An. coluzzii and its sibling species An. quadriannulatus Theobald. As expected, the chemosensory gene repertoire is small in the male maxillary palps. Both species express the tuning receptors Or8 and Or28 at relatively high levels. The CO2 receptor genes Gr22-Gr24 are present in both species as well, although at much lower level than in females. Additionally, several chemoreceptors are species-specific. Gr37 and Gr52 are exclusive to An. coluzzii, whereas Or9 and Gr60 were detected only in An. quadriannulatus. Furthermore, several chemosensory genes show differential expression between the two species. Finally, several Irs, Grs, and Obps that show strong differential expression in the female palps, are absent or lowly expressed in the male palps. While many questions remain about the role of chemosensation in anopheline male biology, these results suggest that the male maxillary palps could have both a sex- and species-specific role in the perception of chemical stimuli. This work may guide future studies on the role of the male maxillary palp in these species.
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Mandiana Diakite, Mory, Juan Wang, Suliman Ali et Man-Qun Wang. « Identification of chemosensory gene families in Rhyzopertha dominica (Coleoptera : Bostrichidae) ». Canadian Entomologist 148, no 1 (7 mai 2015) : 8–21. http://dx.doi.org/10.4039/tce.2015.13.

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AbstractChemoreception is a key process for insects. Odorant messages diffuse through the air and are translated into physiological signals by chemosensory receptor neurons in sensilla that are mainly located on insect antennae. We sequenced the antenna transcriptome of Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae), which is a serious pest of stored grains throughout regions with warm climates, and performed transcriptome analysis on R. dominica antennae. We obtained 57 million 90-base pair-long reads that we assembled into 37 877 unigenes with a mean size of 1007 base pairs. Predicted protein sequences were matched with Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) (79.1%), Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae) (1.7%), Megachile rotundata (Fabricius) (Hymenoptera: Megachilidae) (1.3%), Acyrthosiphon pisum Harris (Hemiptera: Aphididae) (1.2%), and other (16.7%) homologues. In chemosensory gene families, we identified transcripts that encoded the following putative genes: 12 odorant-binding proteins (OBPs), four pheromone-binding proteins (PBPs), eight chemosensory proteins (CSPs), five sensory neuron membrane proteins (SNMPs), six odorant receptors, and eight ionotropic receptors. The diversity of the predicted OBPs, PBPs, and CSPs are also discussed. These findings will advance our understanding of olfaction process by this pest.
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Du, Hai-Tao, Jia-Qi Lu, Kun Ji, Chu-Chu Wang, Zhi-Chao Yao, Fang Liu et Yao Li. « Comparative Transcriptomic Assessment of Chemosensory Genes in Adult and Larval Olfactory Organs of Cnaphalocrocis medinalis ». Genes 14, no 12 (30 novembre 2023) : 2165. http://dx.doi.org/10.3390/genes14122165.

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The rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae), is a notorious pest of rice in Asia. The larvae and adults of C. medinalis utilize specialized chemosensory systems to adapt to different environmental odors and physiological behaviors. However, the differences in chemosensory genes between the olfactory organs of these two different developmental stages remain unclear. Here, we conducted a transcriptome analysis of larvae heads, male antennae, and female antennae in C. medinalis and identified 131 putative chemosensory genes, including 32 OBPs (8 novel OBPs), 23 CSPs (2 novel CSPs), 55 ORs (17 novel ORs), 19 IRs (5 novel IRs) and 2 SNMPs. Comparisons between larvae and adults of C. medinalis by transcriptome and RT-qPCR analysis revealed that the number and expression of chemosensory genes in larval heads were less than that of adult antennae. Only 17 chemosensory genes (7 OBPs and 10 CSPs) were specifically or preferentially expressed in the larval heads, while a total of 101 chemosensory genes (21 OBPs, 9 CSPs, 51 ORs, 18 IRs, and 2 SNMPs) were specifically or preferentially expressed in adult antennae. Our study found differences in chemosensory gene expression between larvae and adults, suggesting their specialized functions at different developmental stages of C. medinalis. These results provide a theoretical basis for screening chemosensory genes as potential molecular targets and developing novel management strategies to control C. medinalis.
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Thèses sur le sujet "Chemosensory gene"

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Thompson, Stephen Richard. « A study of multiple chemosensory gene homologues in Rhodobacter sphaeroides ». Thesis, University of Oxford, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.436968.

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VALERIO, FEDERICA. « Comparative approaches to study the evolution of chemosensory gene families in Bactrocera ». Doctoral thesis, Università degli studi di Pavia, 2021. http://hdl.handle.net/11571/1392537.

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Li, Zibo. « La chimioréception chez les papillons de nuit : approches évolutives et transcriptomiques ». Electronic Thesis or Diss., université Paris-Saclay, 2024. http://www.theses.fr/2024UPASB031.

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À l'interface entre l'insecte et son environnement, l'olfaction et le goût sont deux modalités sensorielles cruciales dans le comportement reproducteur et la sélection de la plante hôte, jouant un rôle essentiel dans l'adaptation des espèces à leur environnement ainsi que dans le processus de spéciation. Chez les insectes, les substances odorantes sont principalement détectées par les récepteurs olfactifs (OR), tandis que les substances sapides sont principalement détectées par les récepteurs gustatifs (GR). Les deux types de récepteurs sont des récepteurs à sept domaines transmembranaires et forment de grandes familles multigéniques. L'objectif de la thèse est d'étudier comment les informations olfactives et gustatives sont détectées par le système chimiosensoriel des insectes, notamment par leurs ORs et GRs, et comment ce système a évolué. En utilisant comme modèles les papillons de nuit Spodoptera littoralis et Agrotis ipsilon, les deux familles de gènes ont été étudiées d'un point de vue évolutif et fonctionnel par une combinaison de séquençage de l'ARN et d'études fonctionnelles. Le premier chapitre décrypte la trajectoire évolutive d'un récepteur de phéromone crucial chez S. littoralis, issu d'une duplication puis ayant acquis une spécificité de réponse à un composé phéromonal, contrairement au récepteur ancestral qui possédait un spectre de réponse large. Cet exemple de sous-fonctionnalisation pourrait être impliqué dans le processus de spéciation. Le deuxième chapitre présente la déorphanisation d'un OR d'A. ípsilon situé dans un neurone olfactif détectant le composé principal de la phéromone de cette espèce et également activé par des volatils de plantes. À l'aide de la technologie de séquençage de noyaux uniques, des OR candidats responsables de la reconnaissance des volatils de plantes résultant de l'activation des neurones phéromonaux sont proposés. Dans le troisième chapitre, nous avons acquis une meilleure compréhension de l'assemblage du complexe que forme un OR avec son co-récepteur Orco. Le quatrième chapitre décrit la carte d'expression des gènes chimiosensoriels chez S. littoralis dans les tissus sensoriels de chenilles et d'adultes et met en évidence les hétéromères OR ou GR dans les neurones individuels des papillons modèles, ce qui permet de mieux comprendre l'assemblage des sous-unités conduisant à la formation des récepteurs chimiosensoriels. Nos études permettent de mieux comprendre l'expression, la fonction, l'évolution et l'adaptation des gènes chimiosensoriels chez les papillons de nuit
At the interface between the insect and its environment, olfaction and taste are two sensory modalities that are crucial in reproductive behavior and host-plant selection, playing an essential role in the adaptation of species to their environment as well as in the speciation process. In insects, odorants are mainly detected by odorant receptors (ORs), while tastants are mainly detected by gustatory receptors (GRs). Both types of receptors are seven transmembrane domain receptors and form large gene families. The aim of the thesis is to investigate how odorant and tastant information is received by the moth chemosensory system and how this system has evolved. Using the two moth species, Spodoptera littoralis and Agrotis ipsilon, the OR and GR gene families were studied from an evolutionary and functional point of view through a combination of RNA sequencing and functional studies
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Obiero, George Fredrick Opondo. « Genome-wide annotation of chemosensory and glutamate-gated receptors, and related genes in Glossina morsitans morsitans tsetse fly ». University of the Western Cape, 2014. http://hdl.handle.net/11394/4347.

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Philosophiae Doctor - PhD
Tsetse flies are the sole vectors of trypanosomes that cause nagana and sleeping sickness in animals and humans respectively in tropical Africa. Tsetse are unique: both sexes adults are exclusive blood-feeders, females are mated young and give birth to a single mature larva in sheltered habitats per pregnancy. Tsetse use chemoreception to detect and respond to chemical stimuli, helping them to locate hosts, mates, larviposition and resting sites. The detection is facilitated by chemoreceptors expressed on sensory neurons to cause specific responses. Specific molecular factors that mediate these responses are poorly understood in tsetse flies. This study aimed to identify and characterize genes that potentially mediate chemoreception in Glossina morsitans morsitans tsetse flies. These genes included sensory odorant (OR), gustatory (GR), ionotropic (IR), and related genes for odorant-binding (OBP), chemosensory (CSP) and sensory neuron membrane (SNMP) proteins. Synaptic transmission in higher brain sites may involve ionotropic glutamate-gated (iGluR) and metabotropic glutamate-gated (mGluR) receptors. The genes were annotated in G. m. morsitans genome scaffold assembly GMOY1.1 Yale strain using orthologs from D. melanogaster as query via TBLASTX algorithm at e-value below 1e-03. Positive blast hits were seeded as gene constructs in their respective scaffolds, and used as genomic reference onto which female fly-derived RNA sequence reads were mapped using CLC Genomics workbench suite. Seeded gene models were modified using RNA-Seq reads then viewed and re-edited using Artemis genome viewer tool. The genome was iteratively searched using the G. m. morsitans gene model sequences to recover additional similar hit sequences. The gene models were confirmed through comparisons against the NCBI conserved domains database (CDD) and non-redundant Swiss-Prot database. Trans-membrane domains and secretory peptides were predicted using TMHMM and SignalP tools respectively. Putative functions of the genes were confirmed via Blast2GO searches against gene ontology database. Evolutionary relationships amongst and between the genes were established using maximum likelihood estimates using best fitting amino acid model test in MEGA5 suite and PhyML tool. Expression profiles of genes were estimated using the RNA-seq data via CLCGenomics RNA-sequences analysis pipeline. Overall, 46 ORs, 14 GRs, and 19 IRs were identified, of which 21, 6 and 4 were manually identified for ORs, GRs, and IRs respectively. Additionally, 15 iGluRs, 6 mGluRs, 5 CSPs, 15 CD36-like, and 32 OBPs were identified. Six copies of OR genes (GmmOR41-46) were homologous to DmelOr67d, a single copy cis vacenyl acetate (cVA) receptor . Genes whose receptor homologs are associated with responses to CO2, GmmGR1-4, had higher expression profiles from amongst glossina GR genes. Known core-receptor homologs OR1, IR8a, IR25a and IR64a were conserved, and three species-specific divergent IRs (IR10a, IR56b and IR56d) were identified. Homologs of GluRIID, IR93a, and sweet taste receptors (Gr5a and Gr64a) were not identified in the genome. Homolog for LUSH protein, GmmOBP26, and sensory neuron membrane receptors SNMP1 and SNMP2 were conserved in the genome. Results indicate reduced repertoire of the chemosensory genes, and suggest reduced host range of the tsetse flies compared to other Diptera. Genes in multiple copies suggest their prioritization in chemoreception, which in turn may be tied to high specificity in host selection. Genes with high sequence conservation and expression profiles probably relate to their broad expression and utility within the fly nervous system. These results lay foundation for future comparative studies with other insects, provide opportunities for functional studies, and form the basis for re-examining new approaches for improving tsetse control tools and possible drug targets based on chemoreception.
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Lo, Bai-Wei, et 羅百尉. « The evolution of chemosensory gene families in fig wasps (Agaonidae) ». Thesis, 2019. http://ndltd.ncl.edu.tw/handle/ef767d.

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碩士
國立臺灣大學
生態學與演化生物學研究所
107
Pollinating fig wasps (Agaonidae) have one of the most reduced chemosensory genes in insects, which is probably associated with specialized life cycle in obligate mutualism. On the other hand, olfaction plays a crucial role in maintaining host specificity in the fig-fig wasp coevolution. In this thesis, I sequenced genomic and transcriptomic data from two fig wasp species to understand how reduced chemosensory genes maintain host-specificity during species divergence. The first chapter describes the evolutionary relationships of the two studied fig wasps (Wiebesia pumilae and W. sp3), their close species (W. sp1), and their associated hosts (Ficus pumila var. pumila and Ficus pumila var. awkeotsang), which revealed that while originally an endemic species, recent human intervention had resulted in introduced populations along with recurrent host-shifting in W. sp3. Possible mechanism for distinct co-pollinator pattern seen in different fig sexual systems was also proposed. The second chapter provides bioinformatics pipelines to generate high quality nuclear genomes and mitochondrial genomes of both species using next generation sequencing, and assess the evolutionary rates in protein-coding genes between them. The final chapter characterizes chemosensory gene evolution of fig wasp from multiple evolutionary perspectives. For fine scale evolution, utilizing the genome and transcriptome of W. sp3 and W. pumilae, both of which codiverged recently with their host, I discovered that regulatory changes at copy-number conservative chemosensory genes are associated with local coadaptations. For large scale evolution, by comparing the two Wiebesia species with Ceratosolen solmsi, I found that lineage-specific adaptive tandem gene duplications in olfactory receptors (OR) family may drive phenotypic coevolution with figs. For olfactory evolution in wasps belonging to different sexual systems of hosts, larger expanded gene families were found in the ancestrally contracted gene families: OR, gustatory receptor (GR) and odorant-binding protein (OBP) in the monoecious fig wasp Elisabethiella stueckenbergi, possibly reflecting differences in host-shifting frequency.
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Nokes, Eva B. « Cis-regulatory mechanisms regulating gene expression in C. elegans chemosensory neurons / ». 2010.

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Wanner, Kevin W. « Characterization of the chemosensory protein gene family from the Eastern spruce budworm, Choristoneura fumiferana ». Thesis, 2004. http://hdl.handle.net/2429/17326.

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The peripheral sensory system of insects is the first to detect chemical stimuli; it is composed of specialized sensory neurons located within hollow, hair-like sensilla. Chemosensory proteins (CSPs) and odorant binding proteins (OBPs) are small, soluble proteins that transport hydrophobic stimuli across the hydrophilic lymph that separates the sensory receptors from the external environment. Incidental results from various studies indicate that most CSPs, and some OBPs, are expressed broadly in many different tissues, raising the question 'what is their non-sensory function?' In this thesis I explored the nonsensory function of CSPs using three different scopes of investigation: 1) an in silico analysis of all known CSP sequences, 2) a characterization of the expression pattern of four CSP genes from a representative lepidopteran species, and 3) a functional characterization of an individual CSP. I identified 15 new CSP sequences; four from cDNA clones described herein and 11 from sequence databases. Several protein similarity classes, representing CSPs from six insect orders, were identified, and each was characterized by highly conserved sequence motifs, including (A) N-terminal YTTKYDN(V/I)(WD)(L/V)DEIL, (B) central DGKELKXX(I/L)PDAL, and, (C) C-terminal KYDP. Three similarity classes were identified that diverged from these conserved motifs, presumably because they are under new functional and selective pressures. A detailed analysis of the expression pattern of four CSP genes from the Eastern spruce budworm, Choristoneura fumiferana, revealed that one (characterized by the retention of the conserved motifs) was expressed in the adult stage, while two that diverged from the conserved motifs were expressed in the immature stages (larvae and pupae). Furthermore, two of the divergent CSP genes were up-regulated during a natural molt, or during an ecdysteroid agonist induced molt. The ligand binding specificity of CfumAY624538, a divergent CSP, was characterized using the fluorescent reporter 1-NPN. Some CSPs bind to medium chain-length fatty acids; this was not the case for CfumAY624538, rather, a short chain-length alcohol was the only ligand tested that displaced 1-NPN in competition. Collectively, my results indicate that divergent CSPs from the Eastern spruce budworm function in development, including larval molting.
Land and Food Systems, Faculty of
Graduate
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Thorne, Natasha. « The Drosophila Gustatory Receptor Genes the Molecular Basis of Taste Perception and Coding ». Diss., 2007. http://hdl.handle.net/10161/392.

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Chapitres de livres sur le sujet "Chemosensory gene"

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Getchell, Thomas V., et Frank L. Margolis. « The Xenopus Oocyte as an in Vitro Translation and Expression System for Chemosensory — Specific Gene Products ». Dans Chemosensory Information Processing, 87–95. Berlin, Heidelberg : Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-75127-1_6.

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Shi, P., et J. Zhang. « Extraordinary Diversity of Chemosensory Receptor Gene Repertoires Among Vertebrates ». Dans Results and Problems in Cell Differentiation, 57–75. Berlin, Heidelberg : Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/400_2008_4.

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Tsien, Roger Y. « New Fluorescent Readouts for Protein Interactions, Gene Expression, and Membrane Potential ». Dans Chemosensors of Ion and Molecule Recognition, 17–21. Dordrecht : Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-3973-1_2.

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Niimura, Yoshihito. « Identification of Chemosensory Receptor Genes from Vertebrate Genomes ». Dans Pheromone Signaling, 95–105. Totowa, NJ : Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-619-1_7.

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Niimura, Yoshihito. « Evolution of Chemosensory Receptor Genes in Primates and Other Mammals ». Dans Post-Genome Biology of Primates, 43–62. Tokyo : Springer Tokyo, 2011. http://dx.doi.org/10.1007/978-4-431-54011-3_4.

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Picimbon, Jean-François. « Bioinformatic, genomic and evolutionary analysis of genes : A case study in dipteran CSPs ». Dans Odorant Binding and Chemosensory Proteins, 35–79. Elsevier, 2020. http://dx.doi.org/10.1016/bs.mie.2020.05.012.

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Shiota, Yusuke, et Takeshi Sakurai. « Silencing of OBP genes : Generation of loss-of-function mutants of PBP by genome editing ». Dans Odorant Binding and Chemosensory Proteins, 325–44. Elsevier, 2020. http://dx.doi.org/10.1016/bs.mie.2020.05.009.

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Actes de conférences sur le sujet "Chemosensory gene"

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Macharia, Rosaline Wanjiru. « Comparative analysis of chemosensory gene families in five tsetse fly species ». Dans 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.114906.

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Snyder, Julia L. « A survey of chemosensory gene expression patterns within the vampire moth genusCalyptraOchsenheimer (Lepidoptera : Erebidae : Calpinae) ». Dans 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.112580.

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Wang, Ran. « Candidate chemosensory protein genes in whiteflyBemisiatabaci by transcriptome analysis ». Dans 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.114182.

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Paulo, Daniel F. « De novotranscriptome assembly of the screwworm flyCochliomyia hominivorax(Diptera : Calliphoridae) : Perspectives for the identification of chemosensory genes and potential microRNAs targets ». Dans 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.112853.

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