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Littérature scientifique sur le sujet « Chemiluminescent immunoassays »

Auteur : Grafiati

Publié le 9 mars 2023

Mis à jour le 10 mars 2023

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Articles de revues sur le sujet "Chemiluminescent immunoassays"

Kricka, L. J., et T. P. Whitehead. « Chemiluminescent and bioluminescent immunoassays ». Journal of Pharmaceutical and Biomedical Analysis 5, n^o 8 (janvier 1987) : 829–33. http://dx.doi.org/10.1016/0731-7085(87)80101-2.

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Kricka, L. J., et G. H. G. Thorpe. « Enhanced chemiluminescent enzyme immunoassays ». Parasitology Today 2, n^o 4 (avril 1986) : 123–25. http://dx.doi.org/10.1016/0169-4758(86)90047-5.

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Tao, Xiaoqi, Song Zhou, Xiameng Yuan et Hongjun Li. « Determination of chloramphenicol in milk by ten chemiluminescent immunoassays : influence of assay format applied ». Analytical Methods 8, n^o 22 (2016) : 4445–51. http://dx.doi.org/10.1039/c6ay00792a.

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Development of 10 chemiluminescent immunoassays for the detection of CAP for a comparison study. First systematic assessment in sensitivity and robustness of different immunoassay formats based on diverse combinations of coating, reverse reaction and antibody source.

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Huang, Xianzhang, David C. Spink, Erasmus Schneider, Helen Ling, Alex J. Rai, Thomas G. Rosano, Baorong Chen et Zhimin (Tim) Cao. « Measurement of Unconjugated Estriol in Serum by Liquid Chromatography–Tandem Mass Spectrometry and Assessment of the Accuracy of Chemiluminescent Immunoassays ». Clinical Chemistry 60, n^o 1 (1 janvier 2014) : 260–68. http://dx.doi.org/10.1373/clinchem.2013.212126.

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Abstract BACKGROUND Unconjugated estriol (uE3) is routinely analyzed in clinical laboratories as risk assessment for Down syndrome. Immunoassays of various types are the most commonly used methods. The accuracies of RIAs and ELISAs for uE3 have been questioned, and to date there have been no independent studies investigating the accuracy of the relatively new chemiluminescent immunoassays. We developed and validated a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for uE3 measurements in serum. METHODS Serum samples from patients in the second trimester of pregnancy were used, and uE3 concentrations were measured by LC-MS/MS and the Beckman Coulter Access® 2 and Siemens IMMULITE 2000 automatic chemiluminescent immunoassay analyzers. RESULTS The LC-MS/MS method was validated and showed limit of detection 0.05 ng/mL; limit of quantification 0.2 ng/mL; linearity of response to 32 ng/mL; total imprecision of 16.2%, 10.4%, and 8.2% for uE3 at 1.10, 4.18, and 8.32 ng/mL, respectively; and analytical recoveries of 95.9%–104.2%. ANOVA of the correlation for LC-MS/MS results vs chemiluminescent immunoassays results showed R2 = 0.9678 (Access 2 = 0.9305 LC-MS/MS + 0.2177, Sy|x = 0.1786, P < 0.0001), and R2 = 0.9663 (IMMULITE 2000 = 0.8849 LC-MS/MS − 0.0403, Sy|x = 0.1738, P < 0.0001). Bland–Altman plots of uE3 results revealed concentration-dependent immunoassay biases. Mock risk analysis for Down syndrome showed no apparent difference in the risk assessment outcomes if the adjusted method-specific multiples of the median were used, and the assay imprecision was <10% CV. CONCLUSIONS Standardization of immunoassay methods for uE3 analysis is needed to improve the accuracy of the measurements.

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Klingler, Wolfgang, Gabriele Wiegand et Rudolf Knuppen. « Chemiluminescent labels for steroid immunoassays ». Journal of Steroid Biochemistry 27, n^o 1-3 (janvier 1987) : 41–45. http://dx.doi.org/10.1016/0022-4731(87)90292-5.

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Boland, J., G. Carey, E. Krodel et M. Kwiatkowski. « The Ciba Corning ACS:180 benchtop immunoassay analyzer ». Clinical Chemistry 36, n^o 9 (1 septembre 1990) : 1598–601. http://dx.doi.org/10.1093/clinchem/36.9.1598.

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Abstract We describe a new clinical laboratory instrument, the ACS:180, used to automate heterogeneous immunoassay testing. The ACS:180 automates immunoassays in which paramagnetic particles are the solid phase and changes in chemiluminescence are measured. The system can accommodate both competitive and sandwich-type assay configurations. The microprocessor-based instrument fully automates each step of the assay, including sample and reagent addition, separation and wash of paramagnetic particles, and generation and acquisition of the chemiluminescent signal. The instrument has the flexibility to operate in random-access or batch mode. The time from application of sample to first result is less than 15 min; throughput is as much as 180 tests per hour.

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Bronstein, I., J. C. Voyta, G. H. Thorpe, L. J. Kricka et G. Armstrong. « Chemiluminescent assay of alkaline phosphatase applied in an ultrasensitive enzyme immunoassay of thyrotropin. » Clinical Chemistry 35, n^o 7 (1 juillet 1989) : 1441–46. http://dx.doi.org/10.1093/clinchem/35.7.1441.

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Abstract We compared a chemiluminescent assay and a colorimetric endpoint assay for measuring an alkaline phosphatase (EC 3.1.3.1) label in an enzyme immunoassay of thyrotropin (TSH). The substrate in the chemiluminescent assay is a derivative of adamantyl 1,2-dioxetane phosphate. On dephosphorylation, catalyzed by alkaline phosphatase, the 1,2-dioxetane decomposes further and emits a glow of light (lambda max 470 nm). We modified the Hybritech Tandem-E TSH High Sensitivity assay for chemiluminescent detection of bound alkaline phosphatase label by using this substrate (with 20-, 40-, and 60-min incubations). Detection limits (mean +2 SD of zero standard) were 6.0, 5.2, and 4.5 micro-int. units/L for these incubation periods, respectively, vs 20 micro-int. units/L for the conventional colorimetric version of the assay. Comparison of results for 44 clinical specimens assayed by the chemiluminescent (20-min incubation, y) and colorimetric (60-min endpoint, x) TSH immunoassays gave statistical values of: slope = 1.17, intercept = -0.22, and r = 0.98. Hemoglobin, but not bilirubin, lipids, or protein, interfered; but these interferents were removed by the washing steps in the enzyme immunoassay.

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Omi, Kazuya, Tsuyoshi Ando, Takuya Sakyu, Takashi Shirakawa, Yoshiaki Uchida, Asako Oka, Nobuyuki Ise, Katsumi Aoyagi et Katsutoshi Goishi. « Noncompetitive Immunoassay Detection System for Haptens on the Basis of Antimetatype Antibodies ». Clinical Chemistry 61, n^o 4 (1 avril 2015) : 627–35. http://dx.doi.org/10.1373/clinchem.2014.232728.

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Abstract BACKGROUND Small molecules classified as haptens are generally measured by competitive immunoassay, which is theoretically inferior to noncompetitive sandwich immunoassay in terms of sensitivity and specificity. We created a method for developing sandwich immunoassays to measure haptens on the basis of antimetatype antibodies. METHODS We generated antimetatype monoclonal antibodies against a hapten–antibody immunocomplex using an ex vivo antibody development system, the Autonomously Diversifying Library (ADLib) system. We selected 2 haptens, estradiol (E2) and 25-hydroxyvitamin D [25(OH)D], as analytes. Sandwich immunoassays for these 2 haptens were developed by use of a 96-well microtiter plate and a fully automated chemiluminescence analyzer, and the performances of these immunoassays were investigated. RESULTS The developed assays exhibited sensitivity high enough to detect target haptens in serum samples. The limit of detection of the ELISA for E2 was 3.13 pg/mL, and that of the fully automated chemiluminescent enzyme immunoassay (CLEIA) system was 2.1 ng/mL for 25(OH)D. The cross-reactivity with immunoreactive derivatives was effectively improved compared with the competitive assay. The CVs for the sandwich ELISA for E2 were 4.2%–12.6% (intraassay) and 6.2%–21.8% (total imprecision). The CVs for the sandwich CLEIA for 25(OH)D were 1.0%–2.3% (intraassay) and 1.9%–3.5% (total imprecision). In particular, the sandwich CLEIA for 25(OH)D showed correlations of r = 0.99 with both LC-MS/MS and a commercially available 125I RIA. CONCLUSIONS Our method represents a potentially simple and practical approach for routine assays of haptens, including vitamins, hormones, drugs, and toxins.

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Wang, Hui, Xiaohui Bi, Lei Xu et Yirong Li. « Negative interference by rheumatoid factor in alpha-fetoprotein chemiluminescent microparticle immunoassay ». Annals of Clinical Biochemistry : International Journal of Laboratory Medicine 54, n^o 1 (28 septembre 2016) : 55–59. http://dx.doi.org/10.1177/0004563216636646.

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Background Rheumatoid factor causes positive interference in multiple immunoassays. Recently, negative interference has also been found in immunoassays in the presence of rheumatoid factor. The chemiluminescent microparticle immunoassay is widely used to determine serum alpha-fetoprotein. However, it is not clear whether the presence of rheumatoid factor in the serum causes interference in the chemiluminescent microparticle immunoassay of alpha-fetoprotein. Methods Serum alpha-fetoprotein was determined using the ARCHITECT alpha-fetoprotein assay. The estimation of alpha-fetoprotein recovery was carried out in samples prepared by diluting high-concentration alpha-fetoprotein serum with rheumatoid factor-positive or rheumatoid factor-negative serum. Paramagnetic microparticles coated with hepatitis B surface antigen–anti-HBs complexes were used to remove rheumatoid factor from the serum. Results The average recovery of alpha-fetoprotein was 88.4% and 93.8% in the rheumatoid factor-positive and rheumatoid factor-negative serum samples, respectively. The recovery of alpha-fetoprotein was significantly lower in the rheumatoid factor-positive serum samples than in the rheumatoid factor-negative serum samples. In two of five rheumatoid factor-positive samples, a large difference was found (9.8%) between the average alpha-fetoprotein recoveries in the serially diluted and initial recoveries. Fourteen rheumatoid factor-positive serum samples were pretreated with hepatitis B surface antigen-anti-HBs complex-coated paramagnetic microparticles. The alpha-fetoprotein concentrations measured in the pretreated samples increased significantly. Conclusions It was concluded that the alpha-fetoprotein chemiluminescent microparticle immunoassay is susceptible to interference by rheumatoid factor, leading to significantly lower results. Eliminating the incidence of negative interference from rheumatoid factor should be an important goal for immunoassay providers. In the meantime, laboratorians must remain alert to the negative interference by rheumatoid factor, and in some cases, pretreat rheumatoid factor-positive samples with blocking or absorbing reagents.

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Mičková, B., P. Rauch, A. Montoya, E. Ferri, F. Fini et S. Girotti. « The determination of N-methylcarbamate pesticides using enzyme immunoassays with chemiluminescent detection ». Czech Journal of Food Sciences 22, SI - Chem. Reactions in Foods V (1 janvier 2004) : S280—S282. http://dx.doi.org/10.17221/10681-cjfs.

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In the present work, enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection for the determination of carbofuran, carbaryl and methiocarb were developed and the analytical parameters of these assays were compared with those of ELISAs with colorimetric detection. The sensitivity of immunochemical methods was expressed as detection limit, linear working range, and I<sub>50</sub> value. In comparison with colorimetric ELISA, the ability of the chemiluminescent reagents to detect lower concentrations of HRP allowed to decrease the optimal antibody and conjugate concentrations and to reach better analytical parameters. The experimental comparison of the analytical performance of the ELISAs was carried out by analysing simply diluted fruit juices, spiked at different concentration levels with the above mentioned pesticides. Recovery values for both ELISAs were around 100% and no matrix effects were observed when fruit juices were diluted 1:20 or more.

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Thèses sur le sujet "Chemiluminescent immunoassays"

ROSSINI, CLARA. « Development of high - throughput chemiluminescent immunoassay for the detection of clostridium difficile and chlamydia trachomatis ». Doctoral thesis, Università degli Studi di Milano-Bicocca, 2012. http://hdl.handle.net/10281/28034.

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The aim of my work was to develop high-throughput chemiluminescent immunoassays, working on the automated LIAISON® system. Two semi-quantitative immunoassays were created to diagnose bacterial infections caused by: 1- Clostridium difficile (detection of Toxin A&B in human stool specimens); 2- Chlamydia trachomatis (detection of anti C.trachomatis human IgG in human serum specimens). Clostridium difficile is a gram-positive, spore-forming anaerobic bacillus. It is a major cause of antibiotic-associated diarrhea and colitis. Antibiotic-associated colitis is an infection of the colon commonly found in individuals who have been using antibiotics. It is the most common acquired infection in hospitalized or extended care patients. Clostridium difficile manifests itself by the production of Toxins A&B. The LIAISON® Clostridium difficile Toxin A&B kit could be used as a screening assay in combination with other more specific assay. For the development of this assay 2 MoMAbs anti Toxin A and Toxin B were produced. These were used as solid phase (PMPs) and conjugate, in a 2 steps “sandwich” chemiluminescent immunoassay, in order to detect the 2 C.difficile Toxins. Some parameters of this new assay were analyzed in this work with the following result: high specificity (99.1%), sensitivity (97.7%) and Limit of Detection (200pg/ml). These values show the high performance of the kit, the Limit of Detection in particular is better than the reference kit of Meridian (PTAB). Nine different strains of toxigenic C. difficile (A-/B+ and A+/B+) were tested by spiking a negative stool with the bacteria. All these microorganisms react in the LIAISON® Clostridium difficile Toxins A&B assay, reflecting a positive result. The ability of stool components to cause interference in general was evaluated. Both the 39 organisms (spiked in negative and low positive stools) and 17 compounds normally present in the stool does not exhibited cross reactivity or interference problems. Finally the short time to results (30 minutes), and the high throughput (> 90 tests / h), together with all parameters see above met the necessary criteria for the marketing of this LIAISON® Clostridium difficile Toxin A&B kit. Chlamydia trachomatis is the most prevalent sexually transmitted bacteria worldwide. It is an obligate intracellular pathogen that can cause numerous disease states in both men and women. Both sexes can display urethritis, proctitis, trachoma, and infertility. The bacterium can cause prostatitis and epididymitis in men. In women, cervicitis, pelvic inflammatory disease (PID), ectopic pregnancy, and acute or chronic pelvic pain are frequent complications. C. trachomatis is also an important neonatal pathogen, where it can lead to infections of the eye (trachoma) and pulmonary complications. The human-pathogenic strains of C. trachomatis have been subdivided into 15 serovars. The 4 peptides mixture (Ct A, Ct B, Ct C and Ct D), used in this thesis, derived from the C.trachomatis major outer membrane protein (MOMP) and are currently used by the C. trachomatis ELISA Savyion Kit. These peptides together are capable to specifically reacting with antibodies specific to any one of the 15 serovars. Several attempts have been tried in the aim to develop this assay: 4 coating concentrations of peptides on solid phase were evaluated with 2 different sample volumes, then 2 modified peptides (with cysteines alkylated) were used in order to improve the recognition by the antibodies (present in the C.trachomatis positive samples). The strategy that allowed the correct functioning of the C. trachomatis LIAISON® kit, has involved the use of biotinylated peptides. These were made to react simultaneously with the solid phase (streptavidin PMPs) and human anti C. trachomatis antibodies in the samples (sera). With the following step the IgG anti human IgG are added as a conjugate. The specificity calculated with this assay is 97.7% and the sensitivity is 99.1%; both these parameters largely satisfy the criteria that were indicated necessary for the marketing of this kit, so the goals were successfully achieved.

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Bashirians, George Mamberi. « Metalloporphyrin : catalysed chemiluminescence immunoassay ». Thesis, City University London, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358939.

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Mangru, Shakuntala Devi. « Chemiluminescence detection in microchip capillary electrophoresis and its application to immunoassays ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/mq22633.pdf.

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RIGAMONTI, VALERIA. « Development of a quantitative chemiluminescent immunoassay for the hepatitis B. antigen detection ». Doctoral thesis, Università degli Studi di Milano-Bicocca, 2011. http://hdl.handle.net/10281/19390.

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The surface antigen of the hepatitis B (HBsAg) is the first detectable serological marker in infected patients. It enables to detect the infection in the first stages of the disease allowing a timely therapeutic intervention with a higher chance of success. A quantitative immunoassay will allow the customers to carry out the quantitative determination of the HBsAg at the start of treatment and during the follow up. Mutations that occur in a conformational surface region, called “a” loop, of HBsAg can cause an epitope loss impairing the antibody interaction deriving from host immune response as well as from vaccination and present in commercial immunoassays. DiaSorin new quantitative immunoassay has high sensibility and specificity and the ability to detect HBsAg mutants.

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CHIAPPA, CARLOTTA. « Design and production of a chemiluminescent immunoassay for the early detection of HIV infection ». Doctoral thesis, Università degli Studi di Milano-Bicocca, 2010. http://hdl.handle.net/10281/7486.

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Combined, simultaneous detection of anti-human immunodeficiency virus (anti-HIV) immunoglobulin and HIV core protein distinguishes fourth-generation (combination assay) HIV screening and diagnostic immunoassays from third-generation (double-antigen sandwich) antibody detection immunoassays. Prior to the introduction of fourth-generation assays, commercial immunoassays for blood screening and diagnosis of HIV infection were based either on detection of HIV core (p24) protein or on detection of HIV-specific antibodies, notably those antibodies directed against HIV transmembrane proteins (tmp). Antibodies against these proteins consistently appear during seroconversion of HIV-infected individuals and remain throughout the course of infection. Fourth-generation immunoassays have targeted reduction of the seronegative window period to achieve a continued decrease in the residual risk of transfusion-transmitted HIV infection. Combining antibody and antigen detection in a single immunoassay format achieves a reduction in the seroconversion window because HIV core protein (p24) appears transiently in the blood and has been used as a marker of antigenemia prior to a detectable humoral immune response to HIV infection. Antigen (p24) testing in a combined (fourth-generation) format has been estimated to reduce the seroconversion window by a few days to as much as about 2 weeks in comparison with third-generation single-format antibody detection assays. Despite enhanced seroconversion sensitivity, fourth-generation assays will be most valuable only if the specificity and sensitivity of individual antibody and antigen detection formats are not compromised when they are combined into a single immunoassay. Sensitive combination assays will need to detect antigen at levels equivalent to those of single-format antigen assays in order to be recommended as replacements for current antigen tests. This prerequisite, however, presents a considerable technical challenge not met by all combination assays. In addition, not all combined formats have achieved the low (non confirmed) repeat-reactive rates expected of blood donor screening and diagnostic assays that result in high specificities such as those typically displayed by third-generation single-format antibody assays. With this work we produced a fourth generation chemiluminescent immunoassay for the detection of HIV-1 and HIV-2 infections, achieving an antigen assay sensitivity approaching that of single-format antigen tests, a specificity equivalent to that of a third-generation single-format antibody assay, and a high degree of total precision.

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REBUZZINI, GABRIELE. « Study of the hepatitis C virus NS3 helicase domain for application in a chemiluminescent immunoassay ». Doctoral thesis, Università degli Studi di Milano-Bicocca, 2009. http://hdl.handle.net/10281/7477.

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The helicase domain of the hepatitis C virus (HCV) protein NS3 is a major immunodominant region that elicits a strong and specific immune response in the majority of HCV infections. Use of NS3 helicase-derived antigens in immunometric diagnostic assays is a mainstay in detection of HCV infections. However, thermal instability of NS3 helicase-related polypeptides is a common and severe issue hampering reproducibility, performances and shelf-life of HCV diagnostic immunoassays. In this work, a novel NS3 helicase-derived antigen is presented, with a sensible improvement in thermal stability and diagnostic performances over the currently used NS3 helicase-related antigens.

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Sentic, Milica. « Electrogenerated chemiluminescence : from mechanistic insights to bioanalytical applications ». Thesis, Bordeaux, 2015. http://www.theses.fr/2015BORD0307/document.

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La chimiluminescence électrogénérée (ECL) est une technique analytique puissante exploitée pour la détection autant au niveau industriel que dans le domaine de la recherche scientifique ou du diagnostic clinique. La sensibilité élevée et la bonne sélectivité de cette technique font de l'ECL une méthode analytique de choix pour un large éventail d'applications, dont la plus importante est son utilisation commerciale dans un grand nombre de tests immunologiques à base de billes fonctionnalisées. Dans cette thèse, nous avons cherché à étudier le phénomène ECL et son application pour le développement de nouvelles techniques analytiques.Dans la première partie de ce travail, nous utilisons les techniques d'imagerie pour étudier les mécanismes ECL se produisant sur les billes utilisées pour les tests immunologiques. La cartographie de la réactivité au niveau d'une seule microparticule fonctionnalisée avec un complexe de ruthénium fournit une nouvelle stratégie visant à tester l'efficacité du co-réactif et montre des effets optiques associés de focalisation.Dans la deuxième partie, la conception d'un test immunologique pour la détection de l'anti-transglutaminase pour le diagnostic de la maladie coeliaque est présentée en utilisant des ensembles de nanoélectrodes comme plates-formes bioélectroanalytiques. Nous avons également étudié les caractéristiques de l'ECL générée par des réseaux de nanoélectrodes dopées au bore-diamant en tant que matériaux prometteurs pour des applications biologiques ainsi que l'efficacité ECL de deux co-réactifs sur ces réseaux.L'électrochimie bipolaire est un processus sans contact que nous avons exploité pour contrôler le mouvement d'objets conducteurs exposés à un champ électrique en l'absence de contact ohmique direct. Dans la troisième partie de ma thèse, nous présentons l'ECL couplée à l'électrochimie bipolaire pour le suivi d’objets autonomes luminescents. Nous avons élargi ce concept à la détection enzymatique dynamique de glucose en utilisant l'émission de lumière ECL comme signal analytique
Electrogenerated chemiluminescence (ECL) is a powerful analytical technique exploited for clinical, industrial and research applications. The high sensitivity and good selectivity, makes ECL a tool-of-choice analytical method for a broad range of assays, most importantly for a large number of commercialized bead-based immunoassays. In the present thesis, we aimed to study the ECL phenomenon and its application in development of new analytical methods.In the first part of this work, we used an imaging technique to investigate the ECL mechanisms operating in bead-based assays. Spatial reactivity mapping at the level of a single functionalised bead provides a new strategy to test the co-reactant efficiency and shows associated optical focusing effects.In the second part, the design of a novel anti-transglutaminase ECL immunoassay for celiac disease diagnostic is shown using nanoelectrode ensembles as bioelectroanalytical platforms. We also studied the characteristics of ECL generated by arrays of boron-doped-diamond nanoelectrodes (BDD NEAs) as a promising materials for bioapplications. The ECL efficiency of two co-reactants at BDD NEAs was investigated.Finally, bipolar electrochemistry is a ‘‘wireless’’ process that was exploited for the controlled motion of conductive objects exposed to an electric field in the absence of direct ohmic contact. In the third part of the thesis, we report ECL coupled to bipolar electrochemistry for tracking the autonomous trajectories of swimmers by light emission. We further expanded this concept for dynamic enzymatic sensing of glucose concentration gradient using ECL light emission as an analytical readout

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Ghosh, Sthitodhi. « A Mobile Healthcare (mHEALTH) System Using Polymer Lab-On-A-Chip With Chemiluminescence Based High-Sensitive Immunoassay For Clinical Diagnostics ». University of Cincinnati / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1592170905649462.

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Kai, Junhai. « Protein Lab-on-a-Chips on Polyer Substrates for Point-of-Care Testing (POCT) of Cardiac Biomarkers ». University of Cincinnati / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1155818041.

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Anderson, Brenda Carol. « Non-Lethal Methods for Assessing Reproductive Status in Bonnethead Sharks (Sphyrna tiburo) ». UNF Digital Commons, 2015. http://digitalcommons.unf.edu/etd/605.

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Reproductive biology is a necessary element for the management of elasmobranch fisheries. Traditionally, characterization of elasmobranch reproduction has involved lethal sampling to examine gross reproductive structures and development of embryos. However, this method is counterproductive to the conservation of shark populations. One non-lethal alternative is the measurement of serum hormones, which often vary according to reproductive events. Radioimmunoassay (RIA) has been used to measure hormone concentrations in reproductive endocrinology, but can be problematic for researchers. Alternatively, chemiluminescence immunoassays (CLIA) are routinely used for measuring circulating hormone concentrations in low-volume, non-extracted human serum samples. However these assays have not been previously examined for use with elasmobranch blood. In the first component of this study, I examined whether CLIA was a suitable alternative for detecting seasonal profiles of these hormones in the bonnethead, Sphyrna tiburo. This was accomplished by collecting serum from sexually mature male (n = 35) and female (n = 32) bonnetheads , measuring reproductive organs for maturity and reproductive stage, and measuring concentrations of testosterone (T) in males, and 17β-estradiol (E2) and progesterone (P4) in females using RIA and CLIA. CLIA was successfully validated for use with shark serum by assessing parallelism and spike recovery. CLIA-derived measurements were significantly correlated with those obtained with RIA (r = 0.809, 0.773, and 0.908 for T, E2, and P4, respectively; p

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Livres sur le sujet "Chemiluminescent immunoassays"

Gary Harold Gregory Henry Thorpe. Enhanced chemiluminescent assays for horseradish peroxidase and their application in immunoassays. Birmingham : University of Birmingham, 1986.

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Weeks, Ian. Chemiluminescence immunoassay. Amsterdam : Elsevier, 1992.

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G, Svehla, et Weeks Ian, dir. Wilson and Wilson's comprehensive analytical chemistry. Amsterdam : Elsevier, 1992.

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Stott, Richard Andrew Whitfield. The use of catalytic labels in chemiluminescence immunoassay. Birmingham : University of Birmingham, 1987.

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International Symposium on Bioluminescence and Chemiluminescence (6th 1990 Cambridge, England). Bioluminescence and chemiluminescence : Current status : proceedings of the VIth International Symposium on Bioluminescence and Chemiluminescence, Cambridge, September 1990. Chichester : Wiley, 1991.

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Weeks, I. Chemiluminescence Immunoassay. Elsevier Science & Technology Books, 1991.

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Howard, Simon B. The development of a chemiluminescence immunoassay for plasma aldosterone. 1996.

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Chapitres de livres sur le sujet "Chemiluminescent immunoassays"

Weeks, Ian, et J. Stuart Woodhead. « Monoclonal Antibodies in Chemiluminescent Immunoassays ». Dans Clinical Applications of Monoclonal Antibodies, 69–79. Boston, MA : Springer US, 1988. http://dx.doi.org/10.1007/978-1-4613-1573-5_7.

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Stott, Richard A. W. « Enhanced Chemiluminescence Immunoassay ». Dans Springer Protocols Handbooks, 1835–43. Totowa, NJ : Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-198-7_195.

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Stott, Richard A. W. « Enhanced Chemiluminescence Immunoassay ». Dans Immunochemical Protocols, 197–205. Totowa, NJ : Humana Press, 1998. http://dx.doi.org/10.1007/978-1-59259-257-9_19.

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Messeri, Gianni, Claudio Orlando, Anna L. Caldini et Mario Pazzagli. « Separation Free Chemiluminescence Immunoassay ». Dans Nonisotopic Immunoassay, 287–300. Boston, MA : Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5466-6_17.

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Kohen, F., Y. Ausher, S. Gilad, J. De Boever, G. J. R. Barnard et J. B. Kim. « Separation-Required Chemiluminescence Immunoassays for Steroids ». Dans Nonisotopic Immunoassay, 271–86. Boston, MA : Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5466-6_16.

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Faatz, E., A. Finke, H. P. Josel, G. Prencipe, S. Quint et M. Windfuhr. « Chapter 15. Automated Immunoassays for the Detection of Biomarkers in Body Fluids ». Dans Analytical Electrogenerated Chemiluminescence, 443–70. Cambridge : Royal Society of Chemistry, 2019. http://dx.doi.org/10.1039/9781788015776-00443.

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Weeks, Ian, et J. Stuart Woodhead. « Chemiluminescence Immunoassay : A New Diagnostic Technology ». Dans Chronobiotechnology and Chronobiological Engineering, 373–79. Dordrecht : Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3547-1_31.

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Tsuji, Akio, Masako Maeda et Hidetoshi Arakawa. « Chemiluminescence Enzyme Immunoassay and Its Use in Diagnostics ». Dans Diagnostics in the Year 2000, 65–95. Dordrecht : Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-6976-9_6.

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Meyer, Verena Katharina, Daniela Meloni, Fabio Olivo, Erwin Märtlbauer, Richard Dietrich, Reinhard Niessner et Michael Seidel. « Validation Procedure for Multiplex Antibiotic Immunoassays Using Flow-Based Chemiluminescence Microarrays ». Dans Methods in Molecular Biology, 195–212. New York, NY : Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6584-7_13.

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Zhao, Shulin, et Yi-Ming Liu. « A Homogeneous Immunoassay of Thyroxine Based on Microchip Electrophoresis and Chemiluminescence Detection ». Dans Methods in Molecular Biology, 79–85. Totowa, NJ : Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-029-8_8.

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Actes de conférences sur le sujet "Chemiluminescent immunoassays"

GIROTTI, S., F. FINI, P. RAUCH, B. MICKOVA, L. KARASOVA, L. FUKAL, A. ABAD, J. J. MANCLUS, J. V. MERCADER et A. MONTOYA. « CHEMILUMINESCENT IMMUNOASSAYS FOR THE DETECTION OF ORGANOCHLORINE PESTICIDES ». Dans Proceedings of the 11th International Symposium. WORLD SCIENTIFIC, 2001. http://dx.doi.org/10.1142/9789812811158_0085.

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Pires, Nuno M. M., et Tao Dong. « Polycarbazole-based organic photodiodes for highly sensitive chemiluminescent immunoassays ». Dans 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2013. http://dx.doi.org/10.1109/embc.2013.6609846.

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Khalil, Omar S., G. P. Mattingly, K. Genger, J. Mackowiak, J. Butler, C. Pepe, T. F. Zurek et N. Abunimeh. « Automated chemiluminescence immunoassay measurements ». Dans OE/LASE'93 : Optics, Electro-Optics, & Laser Applications in Science& Engineering, sous la direction de Gerald E. Cohn. SPIE, 1993. http://dx.doi.org/10.1117/12.146728.

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Yufeng, Yao, Lu Shizhou, Huang Bo et Zhao Jianwen. « Automated Chemiluminescence Immunoassay Analyzer ». Dans 2010 International Conference on Intelligent Computation Technology and Automation (ICICTA). IEEE, 2010. http://dx.doi.org/10.1109/icicta.2010.180.

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YANG, XIAOLIN. « AN ENHANCED CHEMILUMINESCENT IMMUNOASSAY TO DETECT INSULIN ». Dans Bioluminescence and Chemiluminescence - Progress and Current Applications - 12th International Symposium on Bioluminescence (BL) and Chemiluminescence (CL). WORLD SCIENTIFIC, 2002. http://dx.doi.org/10.1142/9789812776624_0097.

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RUBTSOVA, M. Y. U., J. V. SAMSONOVA, A. A. EZHOV et A. M. EGOROV. « CHEMILUMINESCENT DIPSTICK IMMUNOASSAY FOR MULTIRESIDUE ANALYSIS OF PESTICIDES IN WATER ». Dans Bioluminescence and Chemiluminescence - Progress and Current Applications - 12th International Symposium on Bioluminescence (BL) and Chemiluminescence (CL). WORLD SCIENTIFIC, 2002. http://dx.doi.org/10.1142/9789812776624_0091.

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PAZZAGLI, M., P. PINZANI, B. SALVADORI, S. SALTI, L. PASSERI et A. MANZONI. « DEVELOPMENT OF ALLERGOLOGY TEST IN MICROPLATE IMMUNOASSAY FORMAT BASED ON CHEMILUMINESCENCE ». Dans Bioluminescence and Chemiluminescence - Progress and Current Applications - 12th International Symposium on Bioluminescence (BL) and Chemiluminescence (CL). WORLD SCIENTIFIC, 2002. http://dx.doi.org/10.1142/9789812776624_0094.

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YAMADA, M., M. MATSUMOTO et N. WATANABE. « DEVELOPMENT OF THE ENZYME IMMUNOASSAY USING NEW CHEMILUMINESCENCE SUBSTRATE ». Dans Proceedings of the 13th International Symposium. WORLD SCIENTIFIC, 2005. http://dx.doi.org/10.1142/9789812702203_0115.

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YAMADA, M., K. KITAOKA, M. MATSUMOTO et N. WATANABE. « DEVELOPMENT OF A NEW CHEMILUMINESCENCE SUBSTRATE FOR THE ENZYME IMMUNOASSAY ». Dans Proceedings of the 13th International Symposium. WORLD SCIENTIFIC, 2005. http://dx.doi.org/10.1142/9789812702203_0116.

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Chen, Zhenzhen, Lixin Zhu, Renrong Liu, Wei Meng, Na Wu, Fanfan Yang, Kaihong Li et Yifan Lang. « Determination of deoxynivalenol in grain sorghum by chemiluminescence enzyme immunoassay ». Dans 2016 3rd International Conference on Materials Engineering, Manufacturing Technology and Control. Paris, France : Atlantis Press, 2016. http://dx.doi.org/10.2991/icmemtc-16.2016.19.

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